LOCUS       NZ_AP017615             6078 bp    DNA     circular CON 08-MAY-2024
DEFINITION  Escherichia coli strain 20Ec-P-124 plasmid pMRY16-002_5, complete
            sequence.
ACCESSION   NZ_AP017615
VERSION     NZ_AP017615.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00056130
            Sequence Read Archive: DRR065946, DRR065949
            Assembly: GCF_002357915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Sekizuka,T., Kawanishi,M., Ohnishi,M., Shima,A., Kato,K.,
            Yamashita,A., Matsui,M., Suzuki,S. and Kuroda,M.
  TITLE     Elucidation of quantitative structural diversity of remarkable
            rearrangement regions, shufflons, in IncI2 plasmids
  JOURNAL   Sci Rep 7 (1), 928 (2017)
   PUBMED   28424528
  REMARK    DOI:10.1038/s41598-017-01082-y Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Sekizuka,T., Kawanishi,M., Ohnishi,M., Shima,A., Kato,K.,
            Yamashita,A., Matsui,M., Suzuki,S. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2016) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP Assembly v. 3.0; Celera Assembler v. 8.2
            Genome Coverage                   :: 200x
            Sequencing Technology             :: MiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002357915.1-RS_2024_05_08
            Annotation Date                   :: 05/08/2024 03:26:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,320
            CDSs (total)                      :: 5,193
            Genes (coding)                    :: 4,966
            CDSs (with protein)               :: 4,966
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 99 of 227
            Pseudo Genes (incomplete)         :: 141 of 227
            Pseudo Genes (internal stop)      :: 49 of 227
            Pseudo Genes (multiple problems)  :: 59 of 227
            Pseudo Genes (short protein)      :: 5 of 227
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP017615.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="20Ec-P-124"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="pMRY16-002_5"
                     /geo_loc_name="Japan"
                     /collection_date="2008"
                     /note="Inc type: Col156; strain coidentity: 20Ec-P-124 =
                     MRY16-002"
     gene            10..936
                     /locus_tag="CPZ02_RS28255"
                     /old_locus_tag="MRY16002_p50010"
     CDS             10..936
                     /locus_tag="CPZ02_RS28255"
                     /old_locus_tag="MRY16002_p50010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001314449.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            939..1472
                     /locus_tag="CPZ02_RS28260"
                     /old_locus_tag="MRY16002_p50020"
     CDS             939..1472
                     /locus_tag="CPZ02_RS28260"
                     /old_locus_tag="MRY16002_p50020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168772.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            1694..3421
                     /locus_tag="CPZ02_RS28265"
                     /old_locus_tag="MRY16002_p50030"
     CDS             1694..3421
                     /locus_tag="CPZ02_RS28265"
                     /old_locus_tag="MRY16002_p50030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032490394.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGHGNGGGNGNSGGGSNSSVAAVAFGFPALAAPGAGTLGIAVSGE
                     ALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPKMMSKIVTSLPAETVTNVQV
                     STLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVP
                     SLTVSTVKGLPVSTNLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPIYVSV
                     TDVLTPAQVKQRQDEENRLQQEWNDTHPVEVAERNYEQARAELDQANKDVARNQERQAK
                     AVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVN
                     NKKAAFDAAAKDKSDADAALGAALERRKQKENKEKDSKDKLDKESKRNKPGKATGKGKP
                     VGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFKDSNK
                     TNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMDNIRVTTPKRHIDIHRGK"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3424..3684
                     /locus_tag="CPZ02_RS28270"
                     /old_locus_tag="MRY16002_p50040"
     CDS             3424..3684
                     /locus_tag="CPZ02_RS28270"
                     /old_locus_tag="MRY16002_p50040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000420692.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            3746..3889
                     /locus_tag="CPZ02_RS28275"
                     /old_locus_tag="MRY16002_p50050"
     CDS             3746..3889
                     /locus_tag="CPZ02_RS28275"
                     /old_locus_tag="MRY16002_p50050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            4384..5319
                     /locus_tag="CPZ02_RS28290"
                     /old_locus_tag="MRY16002_p50060"
     CDS             4384..5319
                     /locus_tag="CPZ02_RS28290"
                     /old_locus_tag="MRY16002_p50060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271369.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDLNAPAPNITIKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSTSA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTAETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            complement(5513..5911)
                     /locus_tag="CPZ02_RS28295"
                     /old_locus_tag="MRY16002_p50070"
     CDS             complement(5513..5911)
                     /locus_tag="CPZ02_RS28295"
                     /old_locus_tag="MRY16002_p50070"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222916.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     BGC             1..6078
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4385..5319
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.551"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          11..933
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctgaaaccg tggcaaccta tcacctcagc gttaaatttg gtggcaaggg gcaggctgca
       61 aaccacgccg attacatcga gcgcaaagag aaataccgcg accgtcagga tttggaatat
      121 tccgcacacg gaaacatgcc ggaatgggcg cgagataatc cctcacattt ctggcaggcg
      181 gctgaccagt ttgaacgtgc gaacggttcg acctatcggg aacttgaaat tgcattaccc
      241 cgtgaactga cagcggaaca gcgccttgaa ctggtgcagg attttgtacg tcaggaagct
      301 ggcgaacggc acgcctggag ttttgccatt cacaatccga aagcgagcat tgacggcggc
      361 gaacagcctc atgcgcatat catgatgagc cagcgtgtga atgatggcat tgaacgtaca
      421 ccggaacagt atttccggcg ctataacgcc aggtatcccg agcggggagg tgcgaaaaag
      481 gattccggaa gcctgactct cactcagcaa aaagagcagt tgcgggaatt gcgaaaacgc
      541 tgggaggtga agcacaacga acatatgaga aagcatggct ttgaaagggg cttcattgac
      601 tgccggacgc tgaaagaaca gggcattgaa cgacgtcccg aagtgcatct gggttttgaa
      661 gctgccggac ggcttgatga tgcccagcgt catgacatca tgcaaaaacg gtctgaaccg
      721 gactattccc gccatcttga acaggcgcga acggtcgggc atgacatcag ggaatggctg
      781 gacactccgg ttaagcaaca tgaactggtc agaaggctgc aacatgatgt tgcgcgggag
      841 catggctttg acatgagaga cagagtgagc agacagaacc gaactgaacc tgaacgggtt
      901 aaatcaccgt cacgtggatt ttctatggga ttctgaacat ggccagcaca gaagaaaaat
      961 tactcacgct cctgttatcg gtggagcatc tgcaacatac cgcaatggag caacagaaag
     1021 ctgttgctga ttctctggct gtggcaaggg aaacacttcg cggatatcgg gatatttctg
     1081 acggtgtggc aaaacaggtc agggatgaag tcagaaacga ggtgcagaag ctggatgttg
     1141 cagggctgat aggtgagcgc atcagccagg attttcagcg gctggaagag tcagtcgcaa
     1201 acatgaaggc aaacgcggat gccctgaaca gggatattgc gacaattcac agcaatatcg
     1261 tgcaggaata caacaccctt aaagggtata gctggaaatg gctgggtggg gtgtcagtgg
     1321 cttttctggt tatgttctgt gtgcttggat ggggaatgaa aacaatgctc gacaggaact
     1381 acaacgccac agcggcggtg tatcagaaag ttgtgacact ggaagataaa ctggcggaga
     1441 aacctgtcac gggcaagagc cggaaaaact gacgcgcttc gcttgtccaa cgcctcacga
     1501 cccgcgaaag gatttcgcgt tccgttcacc gttttcatga gtgattcctc cgaactgcca
     1561 aaaaatatcc tccccctgac ttgacagaaa aaataatggc gagtactttt tgatctgtac
     1621 ataaaaccag tggttttatg tacagtatta actatgtaat taattgtttt aacgcttaaa
     1681 agagggaatt tttatgagcg gtggagatgg acgcgaccac aacagtggcg cacataacac
     1741 aggtggtaac attaatggcg gccctacggg gcttggtgga aatggtgggg cttctgacgg
     1801 ctccggatgg agttcggaaa ataacccatg gggtggcggt tctggtagtg gtgttcactg
     1861 gggaggtggc tccggccatg gcaatggcgg ggggaatggt aattccggtg gtggcagcaa
     1921 ttcatccgta gcagcagtgg catttggttt tcctgctttg gcagctcctg gtgccggaac
     1981 actgggtatc gccgtctctg gtgaggcttt atctgcggca atagcggata tctttgcagc
     2041 cctgaaaggt ccgtttaaat tcagtgcatg gggtattgcg ctttacggca ttctgccatc
     2101 tgaaatagca aaagatgacc cgaaaatgat gtcaaagatt gtgacgtcat taccggcaga
     2161 aacagtaaca aatgttcagg tcagtacact tccgctggac caggcaactg ttagcgtgac
     2221 aaaacgtgtg accgatgttg tgaaggacac acgacagcat attgccgtag ttgcaggtgt
     2281 acctatgagt gtgcctgttg tgaatgctaa accaacacgt accccaggtg tatttcacgc
     2341 atcatttcct ggtgtccctt ctctgacagt cagcacggtc aaaggtcttc ctgtgtcaac
     2401 aaaccttccc cgtggtatta cggaggataa gggccggact gccgttcctg caggatttac
     2461 ctttggtggt ggttcacatg aagcggtgat ccgtttcccg aaagaaagtg ggcagaagcc
     2521 gatttatgta tcagtgacag atgttcttac ccctgcacag gtaaaacaac gtcaggatga
     2581 ggaaaatcgc ctccagcagg aatggaatga cacacatcct gtagaagtgg ctgaacgcaa
     2641 ttatgaacag gcccgcgcag aactggatca ggctaataaa gatgttgcca gaaaccagga
     2701 acgacaggct aaagctgttc aggtttataa ttctcgtaaa agtgaacttg atgcagcgaa
     2761 taaaactctt gctgatgcaa aggctgaaat aaaacaattc gagcgatttg cccgagaacc
     2821 aatggctgct ggtcaccgaa tgtggcaaat ggcagggctt aaggcccagc gggcacagac
     2881 ggatgtaaat aataagaagg ctgcatttga tgctgctgca aaagataagt ctgatgctga
     2941 tgctgcgtta ggtgctgcgc tggaacgccg taagcagaag gaaaataaag aaaaggactc
     3001 taaggataaa ttagataagg agagtaaacg gaataagcca gggaaggcga cgggtaaagg
     3061 aaaaccagtt ggtgataaat ggcttgatga tgcgggtaaa gattcaggag ctccaattcc
     3121 tgatcgcatt gctgataagt tgcgtgataa agaatttaaa agttttgacg atttccggaa
     3181 gaaattctgg gaagaagtgt caaaagatcc cgagcttagt aagcaattta aagacagtaa
     3241 taagacgaac attcaaaagg gaaaagcacc ttttgcaagg aagaaagacc aagtaggtgg
     3301 tagggaacgc tttgaattac atcatgataa accaatcagt caggatggtg gtgtctatga
     3361 tatggataat atcagagtga ccacacctaa gcgacatatt gatattcata gaggtaagta
     3421 aaaatggaac tgaaacatag tattagtgat tataccgagg ctgaatttct ggagtttgta
     3481 aaaaaaatat gtagagctga aggtgctact gaagaggatg acaataaatt agtgagagag
     3541 tttgagcgat taactgagca cccagatggt tcagatctga tttattatcc tcgcgatgac
     3601 agggaagata gtcctgaagg gattgttaaa gaaattaaag aatggcgagc tgctaacggt
     3661 aagccaggat ttaaacaggg ctaaaatatg aatgccggtt gtttatggat gaatggctgg
     3721 cattctttca caacaaggag tcgttatgaa aaaaataaca gggattattt tattgcttct
     3781 tgcagtcatt attctggctg catgtcaggc aaactatatc cgggatgttc agggcgggac
     3841 tgtatccccg tcatcaacag ctgaagtgac cggattagca acgcagtaac ccgaaatcct
     3901 ctttgacaaa aacaaagcgt gtcaggctga ttctgatgcg cttttttttg aaatgtcaca
     3961 aaaattccat gcgggagatg agagctaaaa tccccgtgca gaactttcca cccaggggga
     4021 gaaaacttgt cattttgacc tgttcgccct tcggaacagt cgaaatcgat cgcgcatcgc
     4081 tttcgtgcat agttatgcag ccctctaaaa acgattctgg cgcgtttttc tggtttagcc
     4141 tggtgtttcc ttgtcttttt gcgttttttg cgccagaaag cgattgaggg cgttttacag
     4201 ggtacgtaca acgggagtta tggtaaatgg atcgggtttt cgggaaggat cgacaggatt
     4261 tgccgttggg tgtagtgtaa gcgactgaaa aacaaacgcc cctgaaattc gtgtctctcc
     4321 gccaagattg tcacgagaat acaggggcga ggtcggtcga taacctagaa ggaatcgtaa
     4381 cacatgagcg ccgcgcttca atacttcgaa gaaaatttac ccctccgccc ctatcacacg
     4441 gatgatctcg ctttcggcct tcgtatctcc ggtaaagggc gcgcgcttct tgcacggtat
     4501 atccagcaga accaacctca tgcgcagttc tggctggttt ttgacgttga ccgcgagggg
     4561 gctgcgattg actggagcga cctgaacgcc cccgcaccga acatcaccat aaaaaatccc
     4621 gtgaacggtc acgcccacct gctctacgca ctcaatatcg ccgtgagaac cgcgcctgat
     4681 gcttcggtta aggcgctgaa atacgccgcc gcgattgaac gtgcgttgtg tgaaaaactg
     4741 ggcgcagatg taaactacag cggactgatt tgcaaaaatc cgttccatct ggaatggcag
     4801 gtgatggagt ggcgtgagga agcctatacc ctcgatgaac tggctgatta tctcgatttg
     4861 agcacttcag cgcgccgtag catcgataaa cattacggga tggggcgaaa ctgccatctg
     4921 ttcgaaatga cgcgcaaatg ggcttacagg gcgattcgtc agggctggcc agcattctca
     4981 cagtggcttg atgccgtgat tcagcgtgtc gaaatgtaca acgcatcgct tcccgttccg
     5041 ctttcacctc ctgaatgtcg ggctattggc aagagtattg cgaaatacac gcacaggaac
     5101 ttcacggcgg aaactttcgc acagtatgtg gctgatacgc acacgccaga aatacaggcc
     5161 aagagaggca ggaaaggtgg catcgctaaa ggcgaagcct acgatgacaa gcgtttcatg
     5221 gcgctatgta tgctggagaa tggatattct cagaaagcta ttgcggcgat gttggaggtt
     5281 tctactcgaa ccattcgaaa ctggaaaagc ggaaaatagc ctatatcaga taacagcgcc
     5341 tttctggcgt ttttttgagc agtaggtctt ttgccgtttt tccccgttca gccctgcaat
     5401 ggtgctgttt tccgcatgat tgtgctggta gtgctcgccg cagtctcatg accgagcgta
     5461 gcgagcgaat gagcgaggaa gcaccaggga accggaacac ggatggaggc acttacgcgt
     5521 ctgggtttgg ggatgtctcc agatcgtact taatttcctt aagtgcctgt aatatcctgt
     5581 cttttttata gcatagctcg cctgtttttg tgtctaccag tcctgcttcg atcagtagcc
     5641 cagccgcgag aaagagtcgg tgattgcgtt ctcgtcgttg tttttttccc tcgattcgtt
     5701 tttggaccgc ctgttttcgt tcgctctcct gtgcctgtaa gtcgtttttt tccgctgttg
     5761 ccagtacgtg gaggagtttg cgttcctgtt ctgtgagtcc tgcttcgttg tcgagcaggt
     5821 caataatctg tctctgtcgt gcggtctgtt ctccgagctt cctgatgaat ccgatccgtt
     5881 tcttcagcca ttcgatgttg ctgatgcgca tgttcgcggt gctccctggt gttttttgcg
     5941 gtgtgttgtg gtcgtgcagt atatcgcttg cggtcggtgg gtgcaggggg gtatcttccg
     6001 tgctaattcg taaaattaga aggaagcgca cttatatgtt tctctccttc gtcgagaaac
     6061 agtgcgctcc tgcggagg
//
