LOCUS       NZ_AP014871            11769 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Bacillus thuringiensis serovar tolworthi strain Pasteur Institute
            Standard strain plasmid pKK7, complete sequence.
ACCESSION   NZ_AP014871
VERSION     NZ_AP014871.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00030057
            Sequence Read Archive: DRR034927, DRR034928, DRR034929, DRR034930
            Assembly: GCF_001548175.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis serovar tolworthi
  ORGANISM  Bacillus thuringiensis serovar tolworthi
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Kanda,K., Nakashima,K. and Nagano,Y.
  TITLE     Complete Genome Sequence of Bacillus thuringiensis Serovar Tolworthi
            Strain Pasteur Institute Standard
  JOURNAL   Genome Announc 3 (4), e00710-15 (2015)
   PUBMED   26139717
  REMARK    DOI:10.1128/genomeA.00710-15 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11769)
  AUTHORS   Nagano,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2015) Contact:Yukio Nagano Saga Univesity;
            Honjo-machi 1, Saga 840-8502, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: sprai v. 0.9.9.2; Celera Assembler v. 8.3rc1;
            SPAdes v. 3.5.0
            Genome Coverage                   :: 1100x
            Sequencing Technology :: HiSeq 2000; PacBio RS ll; ABI 3130
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001548175.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 02:41:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,077
            CDSs (total)                      :: 6,926
            Genes (coding)                    :: 6,583
            CDSs (with protein)               :: 6,583
            Genes (RNA)                       :: 151
            rRNAs                             :: 12, 12, 12 (5S, 16S, 23S)
            complete rRNAs                    :: 12, 12, 12 (5S, 16S, 23S)
            tRNAs                             :: 110
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 343
            CDSs (without protein)            :: 343
            Pseudo Genes (ambiguous residues) :: 0 of 343
            Pseudo Genes (frameshifted)       :: 137 of 343
            Pseudo Genes (incomplete)         :: 226 of 343
            Pseudo Genes (internal stop)      :: 63 of 343
            Pseudo Genes (multiple problems)  :: 69 of 343
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP014871.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11769
                     /organism="Bacillus thuringiensis serovar tolworthi"
                     /mol_type="genomic DNA"
                     /strain="Pasteur Institute Standard strain"
                     /serovar="tolworthi"
                     /db_xref="taxon:1442"
                     /plasmid="pKK7"
     gene            80..1486
                     /locus_tag="KNN_RS34405"
                     /old_locus_tag="KNN_07166"
     CDS             80..1486
                     /locus_tag="KNN_RS34405"
                     /old_locus_tag="KNN_07166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015386301.1"
                     /translation="MKKLAIIFLCAFCIVSHTIPVFADSPSFQLVGSKNVTIENNGIVN
                     GKYNLIIKVNNMKDVYSFQLSELMLVNDCSFHVGKSKWTRPEQEKNEFPINWNQNGTYA
                     FYIFHNGKVLGYARFKLKGFKENGGGGGETVYWGKGAKYYDLPEIEKPNPEDYADYDNK
                     NDGVCTNEKIPDDKPQGSGDIDESKEDNKPPVDGGGGSDKAVKEVLEKIVSAVNEISKN
                     TGETAKNTGETAKNTGEIKKNTDEIAKNTKEIADAVKDIRDNLKIPDDMDLSIKPVLPE
                     DIKPDKPKQPDKPFEDKQEHFKEGEQEKPPDKLPVAPEPAECWGDVCKDKDNQKEEDMK
                     KEKDMDKEEPMKPDKPMEKEDMKPDKPMTPDKPMEKEDMKPDKPMTPDKPMEKEDMKPD
                     KPMTPDKPMEKEDMKPDKPMTPDKPMDREGEMKKDNDMKQDKPMTPWEEMKPSDEGSEL
                     CWHVVNGKCG"
                     /name="hypothetical protein"
     gene            1453..2880
                     /locus_tag="KNN_RS34410"
                     /old_locus_tag="KNN_07167"
     CDS             1453..2880
                     /locus_tag="KNN_RS34410"
                     /old_locus_tag="KNN_07167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090439.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="WP_228176732.1"
                     /translation="MLACSEWKMRIKKFFIVVPVLLGILFVGDISAFAQGKDVLSGRVG
                     KAQYGSSYITSLTDGNLQTSDTLSNGEVIIDLASPVRVSGFRFTKVGGSSNRMDVYFYT
                     EGATLKREYRNTDSGYLQAIDIKGVKKIGLKNMSNGDYFTLAEVNLYEYESVPPSDITD
                     LKENIKHDSVEFTYKNPKSNFSHLRIYRDGQVLASDVKEEKFTDKGLTPETEHIYKFVS
                     VSPDGLESKGIEKKIKTAKEPLPDEIKDLQEDEITHTYVKFSYKFPKNNFDHVKIYRDD
                     KVIADNVKVEEFVDKGLKGSTVYTYKFVSVSSTGLESNGIVKKVQTDEEPNLNKPSKPS
                     NFDITPKDGSLVVNLLSQNLGVPIKGYHIFIDGVKVNDSLVTSRSYAVKGLQNGQTYGV
                     QIKAVSKWNIESDLSNVVNGIPKVPVIPEVTFSFGLGALVDGITNWFLGIWPIVAFSIA
                     IPLAFIVAFNTKKLFMR"
                     /name="fibronectin type III domain-containing protein"
     gene            2890..3045
                     /locus_tag="KNN_RS38625"
     CDS             2890..3045
                     /locus_tag="KNN_RS38625"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015386302.1"
                     /translation="MIQPVTTKDLIQFLQYAFHAGEDVLFLAFVLMITFSVAVSIRRIM
                     VGYKQS"
                     /name="hypothetical protein"
     gene            3045..3467
                     /locus_tag="KNN_RS34415"
                     /old_locus_tag="KNN_07168"
     CDS             3045..3467
                     /locus_tag="KNN_RS34415"
                     /old_locus_tag="KNN_07168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223697961.1"
                     /translation="MEKFVKFLKGGFEAVIKSLKTFIDFLATPFQYLLDFLKGIFYFIE
                     KLFQVVVAIIKLFVALFQFLGAVIAGFFKTLFLWVQPNFDGGNVSFPSASMKGFKVATD
                     ILLPTGLLTVIPAVVTCFIWYLFVKKLMDLFRGGHH"
                     /name="hypothetical protein"
     gene            3467..3727
                     /locus_tag="KNN_RS34420"
                     /old_locus_tag="KNN_07169"
     CDS             3467..3727
                     /locus_tag="KNN_RS34420"
                     /old_locus_tag="KNN_07169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000992628.1"
                     /translation="MNALIDTIFNPVLGWLAQMAKYLSDLSVPASRPLRISDYLGVFAY
                     LYGWTTVITTLFALVFVYFVCYIIMTYSSLFIRFKDTVKWW"
                     /name="hypothetical protein"
     gene            4148..4510
                     /locus_tag="KNN_RS34425"
                     /old_locus_tag="KNN_07170"
     CDS             4148..4510
                     /locus_tag="KNN_RS34425"
                     /old_locus_tag="KNN_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000880242.1"
                     /translation="MLAKTKRKVQPQFEGHRALIIKEEGTCVVEQVISFDSEILETSTA
                     MLPRENELRLLDDNHGYVYHIYNLEKPARIEAENLKMLRRSSALKGIFDFDTGKKPFDI
                     IGFMPWVIIVLLAIFK"
                     /name="hypothetical protein"
     gene            4524..4886
                     /locus_tag="KNN_RS34430"
                     /old_locus_tag="KNN_07171"
     CDS             4524..4886
                     /locus_tag="KNN_RS34430"
                     /old_locus_tag="KNN_07171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000116007.1"
                     /translation="MSVQEMLTKDILEEEGSNETIETVQLFQFMKKHAVPLTKKQQMAM
                     LLLNENGLSDISLYIAGMKEEVLPLSEYHKTLEKLTLSDRIKGNAKLSHLLKANANPSL
                     SLSANSAKVVKGNELA"
                     /name="hypothetical protein"
     gene            4883..5485
                     /locus_tag="KNN_RS34435"
                     /old_locus_tag="KNN_07172"
     CDS             4883..5485
                     /locus_tag="KNN_RS34435"
                     /old_locus_tag="KNN_07172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024420.1"
                     /translation="MNIMVFEGFLGSGKTFGMSLFAKHYEEKSGCVLYSNYGLIGSKPF
                     VTLDTFHDIAKEKSSILNLDEAHIDLDARSFSSNSVKFFSQLSYYLRKLRCTLFITSPS
                     FDDLDSRIRGITNVLVRVSNDKNYFYYKMYDVQSKRYLKTMRIQKKKAFAIGSKVYDTT
                     AMVSPVQVPDKRQDFMEFLEALKSTAEEYGRQYKHSA"
                     /name="hypothetical protein"
     gene            5556..5747
                     /locus_tag="KNN_RS34440"
                     /old_locus_tag="KNN_07173"
     CDS             5556..5747
                     /locus_tag="KNN_RS34440"
                     /old_locus_tag="KNN_07173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000511841.1"
                     /translation="MGILKKKKFREEVKRINKAHGEMREFLDLLMDRYGLDEEEVKNCE
                     VIKHHFDNLDLMFSQMAK"
                     /name="hypothetical protein"
     gene            6365..6571
                     /locus_tag="KNN_RS34445"
                     /old_locus_tag="KNN_07174"
     CDS             6365..6571
                     /locus_tag="KNN_RS34445"
                     /old_locus_tag="KNN_07174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000365510.1"
                     /translation="MDLPVCNEKEVTVLSKTLKYLRSDQSNQVKVHKEYLEDRGWIFDD
                     LTYIDSNIYVVYKKEVVKRNKAF"
                     /name="hypothetical protein"
     gene            6583..7002
                     /locus_tag="KNN_RS34450"
                     /old_locus_tag="KNN_07175"
     CDS             6583..7002
                     /locus_tag="KNN_RS34450"
                     /old_locus_tag="KNN_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000473093.1"
                     /translation="MFKKFYREYIHVLFNIVAMFFYFSWCLVGAEFNLLTLIAIGIALF
                     IFVKYVSMDKKFVNENIDKLKDKKMIFRKYAISGEDIGYAVFTNFLLFILYLGISVYFK
                     PLSVMALLFAFIVNVSFLMLTLRIMSRLSLKNSKV"
                     /name="hypothetical protein"
     gene            7014..7349
                     /locus_tag="KNN_RS34455"
                     /old_locus_tag="KNN_07176"
     CDS             7014..7349
                     /locus_tag="KNN_RS34455"
                     /old_locus_tag="KNN_07176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000457469.1"
                     /translation="MFAKYYSQDDEFMSPLQFIALGGIMSFLIKFGCISPSVNMLTLFV
                     TFVTCIGLCYFFLLLITKYFYRFLAKHRDNLHVYLCNKYNEKIANRIGLCIGLVYIVGV
                     LVICLLI"
                     /name="hypothetical protein"
     gene            7648..7860
                     /locus_tag="KNN_RS34465"
                     /old_locus_tag="KNN_07178"
     CDS             7648..7860
                     /locus_tag="KNN_RS34465"
                     /old_locus_tag="KNN_07178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000216293.1"
                     /translation="MTVKEKKKTWAFTFTESEIQIIDEIVDIENDRRHSIAREHNLPFK
                     KYNRSTFVLAMIEEKKRKYQEQGEI"
                     /name="hypothetical protein"
     gene            8063..8284
                     /locus_tag="KNN_RS34470"
                     /old_locus_tag="KNN_07179"
     CDS             8063..8284
                     /locus_tag="KNN_RS34470"
                     /old_locus_tag="KNN_07179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000391857.1"
                     /translation="MEELLHKIIDKLDKIDKDIQEIKTDIKAHRIETDDNFIKLYHLVE
                     EKDIEIDVLNKRLFKNEATVERIKREKH"
                     /name="hypothetical protein"
     gene            8298..8576
                     /locus_tag="KNN_RS34475"
                     /old_locus_tag="KNN_07180"
     CDS             8298..8576
                     /locus_tag="KNN_RS34475"
                     /old_locus_tag="KNN_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000331888.1"
                     /translation="MAYSYCVDCERVYNCEDEKCCCGSKKRSVDWEEFAFIMGYPLYPV
                     KKKYGVFHLDLSKRLDADALCDLKADNPELFYYEFKGWKERELKKRN"
                     /name="hypothetical protein"
     gene            8903..9094
                     /locus_tag="KNN_RS34480"
                     /old_locus_tag="KNN_07181"
     CDS             8903..9094
                     /locus_tag="KNN_RS34480"
                     /old_locus_tag="KNN_07181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000048809.1"
                     /translation="MSKVKGIRLNLRVSEDEYNKLQYVKECLLMEGLDLKDSAVIRYAL
                     LQTYKDYKEMNDLLKSRN"
                     /name="hypothetical protein"
     gene            9281..10351
                     /locus_tag="KNN_RS34485"
                     /old_locus_tag="KNN_07182"
     CDS             9281..10351
                     /locus_tag="KNN_RS34485"
                     /old_locus_tag="KNN_07182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410863.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000410863.1"
                     /translation="MEKHIEKLSLSVCVDWLEFTFVYGEEFESICSFLGLDPTVFSKEI
                     DGFHKSYGYLSRYSFEEIHILMHGADNRSRIIMSGSGCRWFETLSSVGWYGLFDRIAFA
                     DEHGFSWIKVDRLDIAIDDFVGYFSVKKLKSKIKRRECLSRWKTSFVVETFDLEQGRLQ
                     GSTIYFGSASSKLRWVVYDKLEEQKKKNKDSDSIQGLEFWTRHELRLKKERADRAVKQL
                     VVNDFSIEEMYFGIINNYISFRVVDKEDSNRNRWNIAPFWRQYIGQASKLRLAEALPVA
                     NVERTFKWLSHSVSKKLYLLSEIFSQDDDTKKEFASYLENKGKQKITKGDLDLIENFPI
                     RDFKEFLKIYNEKCLK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            10512..10817
                     /locus_tag="KNN_RS34490"
                     /old_locus_tag="KNN_07183"
     CDS             10512..10817
                     /locus_tag="KNN_RS34490"
                     /old_locus_tag="KNN_07183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001176491.1"
                     /translation="MQLMKVMLTGCKKVDFTNDNNERIEGVNIHFLTTTDNSEQSGFGY
                     LPSKVFFRKEFIEKFSSLNYPIVAEAIIAFNMSPRGIQTKISDFNILEEFPFTALK"
                     /name="hypothetical protein"
     gene            11411..11563
                     /locus_tag="KNN_RS38630"
     CDS             11411..11563
                     /locus_tag="KNN_RS38630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001113828.1"
                     /translation="MPEFGFRPSMSQLFGNFHMNWSTMMPLLVILFGILFGFFVAHLVK
                     DKFDD"
                     /name="hypothetical protein"
     gene            11605..11742
                     /locus_tag="KNN_RS38635"
                     /old_locus_tag="KNN_07185"
     CDS             11605..11742
                     /locus_tag="KNN_RS38635"
                     /old_locus_tag="KNN_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_171840975.1"
                     /translation="MTYNLADVANGLTNWLGAYWPIIAFSVAIPLTFLVAFNTKDLFTR
                     "
                     /name="hypothetical protein"
     Typing          9282..10351
                     /name="rep_cluster_814"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9282..10348
                     /name="MOBT"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagaagttaa ctataaattt aataaaacaa ttaaatagct gggctaataa cctagctatt
       61 tttgttagga aggattgata tgaaaaagct agccataatt tttttatgtg cattttgtat
      121 tgttagccat acgatccctg tgtttgctga ttcacctagt tttcaattgg taggtagtaa
      181 aaatgtaaca atcgaaaata atgggatagt taatggtaaa tacaatttaa ttattaaagt
      241 taacaatatg aaagatgtat atagttttca gttatcggaa ttgatgcttg ttaatgattg
      301 ctcgttccat gttggtaaat caaagtggac tcgtccagaa caagaaaaaa acgagttccc
      361 tattaactgg aatcagaatg gtacatacgc attttacatt tttcataatg gtaaagtttt
      421 aggctatgca cgttttaagc ttaaaggatt taaagaaaac ggtggcggtg gaggagaaac
      481 tgtatattgg ggcaaaggtg ctaaatacta cgatttacct gaaattgaaa agcctaatcc
      541 tgaggactat gcggattatg ataataagaa tgacggagtt tgtacaaatg aaaaaattcc
      601 agatgataaa ccacaaggca gcggtgatat agatgaatca aaagaagata ataaaccacc
      661 tgtagatggt ggcggtggtt ctgataaagc tgttaaagaa gttttagaaa agattgttag
      721 tgcagtaaat gaaatttcaa agaatacagg tgaaacggct aaaaatacag gtgagacagc
      781 aaagaataca ggtgagatta aaaagaacac cgatgaaata gcaaagaaca caaaagaaat
      841 tgcggatgct gttaaggata ttagggataa tttgaagatt cctgacgata tggatttgtc
      901 gattaaacct gttctacctg aggacattaa gcctgataaa cctaaacaac ctgataagcc
      961 ttttgaagac aaacaagagc actttaaaga gggtgaacaa gagaaaccac ctgacaaact
     1021 tcctgttgct cctgagcccg ctgaatgttg gggggatgtt tgtaaagata aagataatca
     1081 gaaagaagaa gatatgaaga aagaaaaaga tatggataaa gaagaaccaa tgaagcctga
     1141 taaaccaatg gaaaaggaag acatgaaacc agataagcca atgactcctg ataagccaat
     1201 ggaaaaggaa gacatgaagc ctgataagcc aatgactcct gataagccaa tggaaaagga
     1261 agacatgaag cctgataaac caatgactcc tgataaacca atggaaaagg aagacatgaa
     1321 acctgataaa ccaatgactc ctgataagcc tatggatcgg gagggagaaa tgaaaaaaga
     1381 taatgatatg aaacaagata agccaatgac tccttgggaa gaaatgaaac caagtgacga
     1441 ggggagtgaa ctttgttggc atgtagtgaa tggaaaatgc ggataaagaa attttttata
     1501 gtagtacctg tattacttgg gattttgttt gtaggtgata taagtgcatt tgctcaaggt
     1561 aaagatgtat taagtggaag agtaggtaaa gctcaatatg gtagttcata tataacttca
     1621 ctaactgatg gaaatttaca aactagtgat actctttcta atggtgaggt cataattgat
     1681 ttagcttctc ctgttagagt ttcagggttt aggtttacta aagttggtgg ctctagtaat
     1741 aggatggatg tttattttta tacagagggt gctactttaa agcgggaata tagaaatact
     1801 gatagtggtt atctacaagc cattgatatt aaaggtgtta aaaaaattgg attgaaaaat
     1861 atgagcaatg gagattattt tacacttgct gaagttaatt tatatgagta tgaatctgtt
     1921 cctccatctg atattacgga tttaaaagaa aatattaaac atgattcagt tgaatttact
     1981 tataaaaatc cgaagtcgaa ttttagtcat ttaagaattt atcgtgatgg acaagtgctt
     2041 gctagtgatg tgaaagaaga aaaattcact gataaaggtt taactcctga aacggagcat
     2101 atttataaat ttgtttcagt ttctcctgac ggtcttgaat caaaaggtat agagaaaaag
     2161 ataaagacag cgaaagagcc tttaccagat gaaataaaag atttacagga agatgaaata
     2221 actcatactt atgtgaaatt tagttataag tttcctaaaa ataattttga tcatgtgaaa
     2281 atttatcgtg atgataaggt gattgcggat aatgtgaagg ttgaggaatt tgttgataaa
     2341 ggtctaaaag gaagtactgt atatacttat aaatttgttt cagtttcctc tactggtctt
     2401 gaatcaaatg gaattgtgaa gaaagtacag actgatgaag aacctaatct caataagcct
     2461 tctaaacctt cgaattttga tattacgcca aaagatggct cgttagttgt aaatctatta
     2521 agtcaaaatt taggcgttcc aattaagggt tatcacattt ttattgatgg tgtaaaagtt
     2581 aatgattcac ttgttacaag tcgttcttat gctgttaaag gtttacagaa tggtcaaact
     2641 tacggtgtac agattaaggc ggtttctaag tggaatattg aaagtgattt aagcaatgtt
     2701 gttaatggaa ttcctaaagt accagttatt cctgaggtta cctttagttt tggtttaggt
     2761 gctttagttg atgggataac aaattggttc ctggggatct ggccgattgt ggcatttagt
     2821 attgctattc cattagcgtt tattgttgcg tttaatacga aaaagttgtt tatgaggtag
     2881 gtgcttagtt tgatacaacc agttacaact aaggacttaa tacaatttct ccagtatgcg
     2941 ttccatgctg gggaagacgt attatttcta gcgtttgtat taatgattac attctctgtt
     3001 gctgtaagca ttagaaggat tatggtgggt tataagcaat catgatggag aagtttgtta
     3061 agttcttaaa aggtggattt gaagcggtta taaagtcgtt aaaaacgttt attgattttt
     3121 tagctacacc atttcaatat ctactggatt ttttgaaagg aatcttttat ttcatagaaa
     3181 agctgtttca ggttgtagta gcaattataa aattattcgt tgccttgttt cagtttttag
     3241 gtgctgtaat agcgggattc tttaaaactt tatttctatg ggtacaacct aattttgatg
     3301 gtgggaatgt atcgtttcct agtgcatcaa tgaaaggttt taaagtagct actgatatat
     3361 tacttcccac tggattactt acagttattc cggctgtggt cacatgtttt atttggtact
     3421 tgtttgttaa aaagctcatg gatctattca gaggtggtca tcattaatga atgcgttaat
     3481 tgatacaata tttaatcctg ttcttggttg gcttgcacaa atggcaaagt atttaagtga
     3541 tttaagcgta ccggcaagcc gtcctttgcg aattagtgat taccttggag tatttgctta
     3601 tttatacggt tggacaaccg ttataacgac gttattcgct ttagttttcg tatattttgt
     3661 ttgttacatc atcatgactt acagtagttt atttattcga tttaaagata cagtgaaatg
     3721 gtggtaatga tattgaatat tgtaggacaa gcaattttat taatattttt tacgacagtt
     3781 gtttcttgga tggtaataga tacattgaat ttctttagag gtatagaaga atgatttatg
     3841 caacgttggg gtttattagt tggattttga ttttgatttg tgaagtaaca tcgagaaaag
     3901 gagatttaaa ataatgcagg cttttaatgc gatgtttgag ttatctagtg cggtttgttt
     3961 atctgtattt ctttttatag ttttaccata tatgtttttc aaaaataaaa tgtcaaagtg
     4021 ataaaaacga atgttaaata tcaattagtt tatatagttc gttaaaaaga ttcaaaatgt
     4081 catggcagcg ggactagatc caagagattt tgaaatgaaa aagcgaacgt tttggagggt
     4141 tttataaatg ttagctaaaa caaaacggaa agtacagcca caatttgaag ggcatagagc
     4201 attgattatt aaagaagaag gaacatgtgt agttgaacag gttatatcgt ttgattcgga
     4261 gattttagaa acgagtacag ccatgttacc acgtgagaat gaattgagat tacttgatga
     4321 taatcatggt tatgtgtatc acatttacaa cttagaaaag ccagcaagaa ttgaagcgga
     4381 aaatttgaaa atgttacgtc gttctagtgc attaaaggga atttttgatt ttgatacagg
     4441 gaagaaacct tttgacatta ttggttttat gccttgggta attattgtat tgctagctat
     4501 ttttaaataa tggggtggag taaatgagtg ttcaggaaat gttaacgaaa gatattttag
     4561 aagaagaagg ttcaaacgaa acgattgaga ctgttcaact gtttcaattc atgaaaaaac
     4621 atgctgtgcc attaacaaag aaacaacaaa tggctatgtt attgcttaat gaaaatggat
     4681 tatcggacat ttctttatat attgctggaa tgaaagaaga agtattgcca ttgagtgaat
     4741 accacaagac acttgaaaaa ttaacgcttt ctgaccgaat taaaggtaat gctaaattaa
     4801 gtcatttact gaaagcaaat gcgaatcctt cattatcatt gtcagcaaat agcgcaaaag
     4861 ttgttaaagg gaatgagtta gcatgaacat aatggttttt gagggctttc tcggtagcgg
     4921 taaaacgttt ggtatgagtt tgtttgcaaa acactatgaa gaaaagagtg gctgtgtatt
     4981 gtacagtaat tacggtttaa ttgggtctaa accatttgta actttagata cgtttcatga
     5041 tattgcaaaa gagaaatcta gtatcttaaa tctcgatgaa gcgcatattg atcttgatgc
     5101 tagaagtttt tcaagtaact cggtaaagtt cttttctcaa ttgtcttatt accttagaaa
     5161 actacgttgt acgttgttta ttacctcacc atcatttgat gatttagatt cacgtataag
     5221 aggtattaca aacgttctcg ttagggtatc gaatgataaa aactattttt attacaaaat
     5281 gtatgatgtt caatctaaac gatatttaaa aacaatgcgt attcaaaaga agaaagcttt
     5341 tgctattggt tcaaaagttt atgatacgac agcaatggtt tctccagtac aagttccaga
     5401 taaacgacaa gacttcatgg aatttttaga agcgttgaag tcgaccgccg aagagtacgg
     5461 acgccagtac aagcattcag cgtaagctgt atgcgcggtg caggcgggag gacttcataa
     5521 gattatataa attaaataag gaaggtacgt tatagatggg aattttaaag aagaaaaaat
     5581 ttagagaaga agtgaaaaga ataaataaag cacatggaga aatgagagaa tttctagatt
     5641 tgctcatgga tcgctacgga ttagatgaag aagaagtaaa aaattgtgaa gtaattaaac
     5701 atcattttga taatttagat ttgatgttta gtcaaatggc aaaataatgt tcgtgattta
     5761 ggtgtgaaat gtaacggcag ccgtgcatat tttccagggg cttttaatga aaatacgaac
     5821 attaagtatg aggtgaagag atttgataca tgtagattgg ttgaaaacta tggcgagaga
     5881 cggattattt tatggttcat tcattgctgt aattgttttc ttgctaaatt ggtttacggg
     5941 ttaggtttta aaggtcttcc ttcgcttttg aggttggaga ttggctgggg tgtggggcta
     6001 gccccgcata gcgtaatggg taaatagaat aaaagaaaat ataaaggtcg gtagtacaag
     6061 cgtttaggct cgtataatgg ataggcaagt tgatagttaa atatattcac ttttgagttt
     6121 atcgacttgg cgatgaagac aaacgagcct aaaaaagtta tgcaagaatg tagcgtagcg
     6181 gaaaaggtgg agattttatg aagcagagcg taagaaaata ctacctgttt cttgtttaac
     6241 cttttagcaa atacagatag agggttgttt tgatggagaa tgttattgtt acgttgtttg
     6301 gtactctagg gatttgtttc ttgtttaaag gcatgctaga tactttaaaa gaagggtgat
     6361 atttgtggat ttgcctgttt gtaatgaaaa agaggtaact gttttatcta agacattgaa
     6421 atatttgaga agtgatcaat ctaatcaagt taaggtacat aaagagtatt tagaggacag
     6481 aggttggatt tttgatgatt taacttatat agatagcaat atctatgtag tttataaaaa
     6541 agaggttgta aagcgaaata aggcgtttta aggggtgtat atatgtttaa aaagttttat
     6601 cgtgaatata tccatgtatt gtttaatatt gttgcgatgt ttttttattt tagttggtgt
     6661 ttagttggtg ctgaatttaa tttattaact ctaatagcaa ttggaattgc cctttttatt
     6721 tttgttaagt atgtatctat ggataagaaa tttgttaatg aaaatataga taaattaaaa
     6781 gacaaaaaga tgatttttag gaaatatgct ataagtggtg aagatattgg atatgcagtt
     6841 tttacaaatt ttttattgtt tattttgtat ttaggaatat ccgtatattt taagccttta
     6901 agtgtaatgg ctttattatt tgcttttatt gttaatgtta gttttcttat gttaactctt
     6961 agaataatga gtagattaag tttaaagaat agtaaagttt aagaggtgta tatatgtttg
     7021 ctaagtatta tagccaagat gatgaattta tgtcgccact tcaatttata gctttgggtg
     7081 gtattatgag ttttcttatt aaatttggtt gtatttcgcc tagtgtgaat atgttaacgt
     7141 tatttgttac tttcgttact tgtattggat tatgttattt ttttctactt ttaataacaa
     7201 agtattttta tagatttctt gctaaacatc gtgataattt acatgtttat ttatgtaata
     7261 agtacaatga aaaaatagca aatagaatcg gtttatgtat tggtttagtt tatattgtag
     7321 gggtgttagt tatatgtttg ctgatttaaa gttgaatttt aaattttggt taactgtagt
     7381 ttttgatata tgtatgcttg caatgatctg tgttgttaca acaaagtatg tatctgatat
     7441 atatagtgat ttgtttattt tcacgacagg atttttaata ctattaattt ggtattctgt
     7501 tagagtgtat ttgatagatt taatcagaaa aaaacctatc aataattaaa atttaatatt
     7561 tttttattga aaaaccatat gtttttattt acaaaccata tggtttttgt tatattaaat
     7621 ttagttaatt gataggaggt tgtttttatg acagttaaag aaaagaaaaa aacttgggca
     7681 tttacattta ctgaaagtga aattcagata atagatgaaa ttgtagatat agagaatgat
     7741 agaagacatt caattgctag agaacataat ttaccgttta aaaaatataa tcgttctaca
     7801 tttgtgcttg caatgattga agaaaagaaa cgtaagtatc aagagcaagg agaaatttaa
     7861 tggaaattgt tttaggtgtt agtgcgtttg gtttgtatat ttcgttttta atatttttat
     7921 attggatggc attagatttt ttagcttggt tatataaaaa gatttaataa aatcgttatt
     7981 ttattggttt tctcttaact tgttaatatt aataggggat agagtctaac ttttttaaaa
     8041 ttatattttg gaggtcgtaa ctatggaaga attgttacat aagattattg ataaattaga
     8101 caagattgat aaggatattc aggagattaa aactgatatt aaggctcacc gaattgaaac
     8161 tgatgataat tttattaagc tatatcattt agttgaagaa aaagatattg agattgatgt
     8221 gttgaataag cgtttgttta agaacgaggc gacagttgaa cgtattaaga gggaaaagca
     8281 ttaggaggaa attgtcgatg gcatatagtt attgtgtgga ttgtgagcgt gtgtataact
     8341 gtgaagatga aaagtgttgt tgtggaagta aaaaacgttc tgttgattgg gaagagtttg
     8401 cttttattat gggttatccg ttatatcctg ttaagaaaaa atacggtgtt tttcatttag
     8461 atttatctaa acgtttagat gctgatgctc tatgtgatct taaagcagat aatccagaac
     8521 ttttttatta tgagtttaaa ggttggaaag aaagagaatt gaaaaagaga aattaataat
     8581 aaatattttg gagttagtgg gtgttttgtt tgtctaattc gaaggaaaat ttccagttgc
     8641 gaaatgagga acccccgtcc agtaacacgg gggttcccgg ctgttttgat atctacacta
     8701 tcccttgcct agactgggtt caagtcactt ttaaatctgt acagaattca caaaaaaatg
     8761 cacggaaaat tattagtaaa atttccagtt tttaatgaag cttaaaagat tcttatattg
     8821 gtataatatt tacaaggagg aaaatggaaa ctctttacat atggtgtatg acattgtatg
     8881 cttcaattga ggaggttata taatgtcaaa agttaaaggt attcgtttga atttaagagt
     8941 ttctgaagat gagtataaca agttgcagta tgttaaagaa tgtttactta tggaaggtct
     9001 tgatcttaaa gattcagctg ttattcgtta tgctttatta cagacttata aggattataa
     9061 agaaatgaat gatttgttga aatcaaggaa ttgaatagct gatttcatgt ttctactggg
     9121 ggctagtaac accccagtag aaaatacagg tcaatcacct ccgaacgctt gtggctctag
     9181 ggttcgtcct tctttgtttt tttcacggtt cttttaaaaa ataggcaaaa aattcgcggt
     9241 ttttattttg cttttattgt attgttttag aggtgtttgt atggaaaaac atattgaaaa
     9301 attgtctctt tcggtttgtg tggactggtt ggagtttact tttgtttatg gtgaagagtt
     9361 tgaatctatt tgtagttttt taggtctaga tcctactgtt ttttctaagg aaatagatgg
     9421 ttttcacaag tcttatggtt atttgtctag atattctttt gaagaaatac acattcttat
     9481 gcatggtgct gataatcgtt ctagaattat tatgtctggt tctggttgca gatggtttga
     9541 aacattatcc tctgttggct ggtatggttt gtttgatagg attgcatttg cagacgaaca
     9601 tggtttttct tggattaaag ttgaccgttt agatattgct attgatgatt ttgtaggtta
     9661 tttttctgtt aaaaaattaa agtctaaaat aaaaagaaga gaatgtttga gtcgttggaa
     9721 aacaagtttt gttgttgaaa cttttgattt agaacaggga cgattgcagg gttctacgat
     9781 ttattttgga agtgcttcta gtaaattgag atgggttgtt tatgataagt tagaagagca
     9841 gaaaaaaaag aataaagata gtgattcgat tcaaggttta gaattttgga ctaggcatga
     9901 gttgcgtttg aaaaaggagc gtgctgatcg tgctgttaaa caacttgtag ttaatgattt
     9961 tagtattgaa gaaatgtatt ttggtattat aaataattat atttcgtttc gtgttgtcga
    10021 taaggaagat tctaatagaa ataggtggaa tattgcacct ttttggaggc aatatatagg
    10081 acaagcaagt aaattaagat tagcagaggc tttacctgtt gcaaatgttg agcgtacgtt
    10141 taaatggttg tctcatagtg tttctaaaaa attatatttg ttgtctgaaa ttttttcgca
    10201 agatgatgat acaaagaaag agtttgctag ttatttagaa aataagggca aacaaaaaat
    10261 cacgaaaggg gatttagact taatagaaaa tttcccaatt cgtgacttca aagaattttt
    10321 aaagatttat aatgaaaaat gtctaaaata attttactct attttataat ggaagtctaa
    10381 tacattaaaa aatccatata tttttaaaaa aagactagaa tttttttcta gtttttctta
    10441 taattaaatg tatgacaaag gtgtacatag aacgacattg tatgactaaa aagattctaa
    10501 ggagtgtaaa tatgcaatta atgaaagtaa tgttaacagg ttgtaagaaa gttgatttca
    10561 caaatgataa taatgaaaga atcgaaggtg ttaatattca ctttttaact acaactgata
    10621 atagtgagca atctggtttt ggatatcttc cttctaaggt cttttttaga aaagagttta
    10681 tagaaaaatt tagttctttg aattatccaa ttgttgctga agctattatt gcttttaata
    10741 tgtctcctcg tggtattcaa actaaaattt ctgattttaa tattttagag gaatttcctt
    10801 ttacagcttt aaagtgattt tatgatagat aaaatcgatt taaatgaatt gtacaaaact
    10861 atttcggaaa ttatgcctaa ttacttagtg cagactaaga atggtagttt tgcagttttc
    10921 cagacactta cctatggtga tatgttaatt agtttcttgt tattgctaat cttgttggtg
    10981 ttgacattca actggatatg gagtgcttta cgatgatcga gtttaattta gaaaacttgt
    11041 tgaatcaata ttccttgttt ggattaccat ttttattaat tatcgtgttc acattgctga
    11101 aagattggcg gtttgataga taatgaatga tttagcgttt ctagatatgt tgtattggtt
    11161 cttcatgaac aattcagttg ttttatactc gttaggtttt ataacctttc tagtattcgt
    11221 aataatgatg ttcaccaccg tcaaagcttg gggtgatggc tattagtttt agcatggacg
    11281 tgggaagaat cttacagtat gcctttaaca tgtttagttc ggtgttacca gtgatttatt
    11341 tattcattgg tggggcgttt gctatctact gtatcggtaa agtgtacaac acctttagga
    11401 gttgatttca atgcctgaat ttggattcag accatcgatg tctcagttat tcggtaactt
    11461 tcatatgaac tggtcaacaa tgatgccatt gttagtcatt ttgtttggga tactgtttgg
    11521 atttttcgta gcgcatttag taaaagacaa atttgatgat tgattaatta ggaggaattt
    11581 attttggaaa tgccaaaaat tacattgact tataatcttg cagacgttgc taacggtttg
    11641 actaactggt taggagcata ttggcctatt atcgcattct cggttgcgat tccattaacg
    11701 ttcttagtgg cgtttaacac taaagaccta tttactcgtt aatcatcaaa taatcactcc
    11761 ttaaatagc
//
