LOCUS       NC_025137              11465 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter pittii plasmid pMS32-3, complete sequence.
ACCESSION   NC_025137
VERSION     NC_025137.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226815
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11465)
  AUTHORS   Huang,T.-W., Liao,T.-L., Lai,J.-F., Lin,A.-C., Chen,Y.-T.,
            Tsai,S.-F. and Lauderdale,T.-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2014) National Health Research Institutes,
            Institute of Molecular and Genomic Medicine, 35 Keyan Road, Zhunan
            Town, Miaoli 35053, Taiwan
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.6
            Coverage                          :: 254
            Sequencing Technology             :: 454
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 04:06:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 12
            CDSs (total)                      :: 12
            Genes (coding)                    :: 11
            CDSs (with protein)               :: 11
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KJ616406.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11465
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="MS32"
                     /db_xref="taxon:48296"
                     /plasmid="pMS32-3"
                     /geo_loc_name="Taiwan"
     gene            join(11464..11465,1..286)
                     /locus_tag="HPF19_RS00005"
                     /old_locus_tag="D722_p1001"
     CDS             join(11464..11465,1..286)
                     /locus_tag="HPF19_RS00005"
                     /old_locus_tag="D722_p1001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_171264639.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDVSFETASLVFENPLRISIQDRHTDG
                     EERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERDKYEHG"
                     /name="BrnT family toxin"
     gene            273..587
                     /locus_tag="HPF19_RS00010"
                     /old_locus_tag="D722_p1002"
     CDS             273..587
                     /locus_tag="HPF19_RS00010"
                     /old_locus_tag="D722_p1002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_057095489.1"
                     /translation="MSMVKYSRKELNEKFSDKQDAEIERLLAKGTVSDDHLDLSDMPEI
                     TDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            774..2948
                     /locus_tag="HPF19_RS00015"
                     /old_locus_tag="D722_p1003"
     CDS             774..2948
                     /locus_tag="HPF19_RS00015"
                     /old_locus_tag="D722_p1003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="WP_032072114.1"
                     /translation="MLDFKILNKGIAVIKVIWGTNKEKPIASRQLAETLSSNMSLEGFL
                     YIGYPIVGSPLGPTKFDALLLSESKGIIAFDLVEGTDLTNYINNQDEMASMLEVKLKPY
                     PKLKKGRHLKFDIKTVTFCPAKKNNLPQVEDNLYNIANISNLNEVINSFEWESDEDTFK
                     ELKAAIQVVTNIRSGAIRRQTKKENSLGSRLQKLEDSIANLDQHQSEAVIETVDGVQRI
                     RGLAGSGKTIVLALKVAYLHAQNQSWKIAVTFHTRALKEQFKRLINNFTIEQLGSEPDW
                     DSIDIFNSWGAPGDKENDGMYHRYCVENSIDYYDFQTAKNEFGGDDAFKNVCKLALEQS
                     SSNKIEKYDLILIDEAQDFPPEFIKLCYKFLKEPKRLVYAYDELQSLNGLSVLPPEQLF
                     GKDSKGVPLVTLEEEDPTKPKKDIILEKCYRNSRPLLATAHTLGFGLTRPKGLIQFFDN
                     DSLWEDVGYSVKKGKIEGGKEVELIRTSESSPLFLEEHTPLEELIKFEVFDSVRDMNQM
                     LFDSIVKNIHEDELKPEDILVINPDPFTTQKNVGIVRKALQQNEIDSEIAGVTTSRDIF
                     RKNGSVTFTGIFRAKGNEAAMVYIIHAQDCADSYDPNHLALIRNRLFTAITRSKAWVRV
                     LGIGPSMQALKEEWETLKNNDYALKFVYPTEKQKNTLKLINKDMSVQERNRRRKLTHNI
                     QEIIHAINSGELPTELIPEELINILKKSGH"
                     /name="ATP-binding domain-containing protein"
     gene            2950..3237
                     /locus_tag="HPF19_RS00070"
     CDS             2950..3237
                     /locus_tag="HPF19_RS00070"
                     /inference="COORDINATES: protein motif:HMM:NF021564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2290 domain-containing protein"
                     /protein_id="WP_032072115.1"
                     /translation="MDSDAISKQCTEIIDRLILSGLSIEQSYPSTRMNASGDYEIGFKK
                     GIEISQTLKNIPYEDIYKSVLDEKSYHIHLIDGGLVSFNYIFEKNDDVFL"
                     /name="DUF2290 domain-containing protein"
     gene            3218..3619
                     /locus_tag="HPF19_RS00075"
     CDS             3218..3619
                     /locus_tag="HPF19_RS00075"
                     /inference="COORDINATES: protein motif:HMM:NF021564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2290 domain-containing protein"
                     /protein_id="WP_171264640.1"
                     /translation="MMMFFYKKHRLSFYPSPSLPSFEDCPELYSNDEIFLECTKKNLVK
                     FPIRFDFDPKNHVNVTHPASHVTFGQFLNCRIPVSMPVTPRKFILFLLRNFYYSGYTKS
                     KNIFDKKMVNVIPFHTITSEERKIGHFIL"
                     /name="DUF2290 domain-containing protein"
     gene            complement(3740..4903)
                     /locus_tag="HPF19_RS00030"
                     /old_locus_tag="D722_p1006"
     CDS             complement(3740..4903)
                     /locus_tag="HPF19_RS00030"
                     /old_locus_tag="D722_p1006"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004994878.1"
                     /GO_function="GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]; GO:0004024 - alcohol dehydrogenase
                     activity, zinc-dependent [Evidence IEA]; GO:0008270 - zinc
                     ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="WP_005237355.1"
                     /translation="MRALTYHGARDVRVESVPDPVIQEPDDVILRVTATAICGSDLHLY
                     RGKIPATEDGDIFGHEFMGIVEEVGPEVTEVKKGDRVIIPFVIACGHCFFCEHELMAAC
                     ENTNTGRGAILNKKQIPPGAALFGFSHLYGGIPGGQAEYVRIPKGNVGPFKVPGSLPDE
                     KVLFLTDILPTAWQAVNNAQVGRGSSVAIYGAGPVGLLAAACARMLGAEQIFMVDHHPY
                     RLSFAHQTYGVIPVNFDEVDAAEFIIQNTTGYRGVDAVIDAVGFEAKGSMLETVMTNLK
                     LEGSSGSALRQCIAAVRRGGVVSVPGIYAGPIHGFLFGDAFDKGLTFKMGQTHVHNYLP
                     QLLEHIENGDLSPDLIITHRMKLEDAAEGYRIFDKKEEDCRKVILTP"
                     /name="zinc-dependent alcohol dehydrogenase"
     gene            complement(5147..5479)
                     /locus_tag="HPF19_RS00035"
                     /old_locus_tag="D722_p1007"
     CDS             complement(5147..5479)
                     /locus_tag="HPF19_RS00035"
                     /old_locus_tag="D722_p1007"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032049750.1"
                     /translation="MPKPFLGSADDLAKFLDKHFRLGVKENGSSSFKFLNKGEAYSVLK
                     YVARDVINEEVERLKVEDPKAVARYNPGPSKIRLLDPLKPSTTGYIHIVLKRRYGSGNY
                     CIRPIR"
                     /name="hypothetical protein"
     gene            complement(5564..6559)
                     /locus_tag="HPF19_RS00040"
                     /old_locus_tag="D722_p1008"
     CDS             complement(5564..6559)
                     /locus_tag="HPF19_RS00040"
                     /old_locus_tag="D722_p1008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032049752.1"
                     /translation="MIHYSPMSRYTAQKIVDKVGHGAYFYSHFSVEGEDNLFFPKIDKL
                     IKKLTDKYHLDLTSRQRSYRLNTKKEPIADLIVQKRVNSTIFDFWLLITTPNTHKFNTQ
                     LSQINLKPRLSGQRVAEAENVVWNRENEQQEISVIQDYFRDQEKFKFVLQKPYLKLNFG
                     NGKYVELVRLSHSTKNSKKYASNRKKSEKNYTWTWRYDEPTVHLIEKKYKEIINDLISN
                     PNKSVGIGKWQQLNADLQHYTVFKGNRHQVGRLFTQAVGYHYKKGQSNLRNAEYYQPLT
                     LSYLPRQENYAEDFIQFVILRRLFEETGREFGKENVHEENYNQLINQYLI"
                     /name="hypothetical protein"
     gene            8894..9823
                     /gene="repM"
                     /locus_tag="HPF19_RS00045"
                     /old_locus_tag="D722_p1010"
     CDS             8894..9823
                     /gene="repM"
                     /locus_tag="HPF19_RS00045"
                     /old_locus_tag="D722_p1010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017401951.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005237331.1"
                     /translation="MSKELVVKTNRLNQAFQTLSLSEFHIVQLAIVDARHTGTGLSTDT
                     PLRIDALRYAEVFGTTRQNAYQRMKEAEDSLFNRRFSFFDEDGKLVKSRWIQQVKYLDD
                     EGAIELVFTLAVVQGISKIDGIKDFFTQYLLSQTAQLNSTYSARLYELLIQWRAIGKTP
                     VFELATFREQLGIGVNEYKRMDHFKTRVLDLAISEISEKTDIEATYQQHKKGRSISGFS
                     FSFKQKKSKTKSLENQTISGNLDLFSKKMTDSQRHLFSNKLSELPEMSKYSQGTESYPQ
                     FAVRIAEMLKDSEKLKEFAPMLEKVGYR"
                     /name="repM"
     gene            9892..10437
                     /locus_tag="HPF19_RS00050"
                     /old_locus_tag="D722_p1011"
     CDS             9892..10437
                     /locus_tag="HPF19_RS00050"
                     /old_locus_tag="D722_p1011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_171054282.1"
                     /translation="MKALSVIELAQLYGMNRQSIYKRINKGDLSKNSDGKIDFSEALRV
                     FGEPSHNNKDVTTLQSTSVQKETEVDMLKQQVDILKKQLELAHEREVFQREQLEAKDNQ
                     IEAIQRLLEAPKTNMTTFTDQKPSQDIATDPRSEPEPKHDGLTTPEQTENKRIPVPDHV
                     EPEQPKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(10632..>10766)
                     /locus_tag="HPF19_RS00065"
                     /pseudo=""
     CDS             complement(10632..>10766)
                     /locus_tag="HPF19_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004733157.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /name="cysteine hydrolase"
     gene            10885..11214
                     /locus_tag="HPF19_RS00055"
                     /old_locus_tag="D722_p1012"
     CDS             10885..11214
                     /locus_tag="HPF19_RS00055"
                     /old_locus_tag="D722_p1012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005237346.1"
                     /translation="MKNLQIVIAQALHSYEFEHMEQSISKSISDTILEILVNDGPFEKI
                     DCYLYSKKDSKKILKSSIHYVIAVLERAKYRVQKAQQDNLSPLYICLYVIKKIEGFIEY
                     LNTKF"
                     /name="hypothetical protein"
     Typing          8895..9823
                     /name="rep_cluster_1261"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggaacagtat tttgaatggg atgaggctaa gaatcgaaag aatcagaaaa agcacgatgt
       61 ctctttcgaa actgcaagcc ttgtttttga aaatccgttg aggatctcaa tccaagacag
      121 acataccgat ggcgaagaac gttggcagac cattgggaaa gtaaaaggcg tactaatgct
      181 cttagtagct cacaccatct ttgatgaaga tgactgtgaa atcatacgaa tcattagtgc
      241 aagacaagta actaaagcag agcgagataa atatgagcat ggttaaatac tcacgcaaag
      301 aactgaatga aaaattcagc gacaagcaag atgctgaaat tgaacgcttg cttgctaagg
      361 gaacagtttc tgacgatcat ctagatctat cagatatgcc tgaaattacc gactggagta
      421 atgctgtacg ccacaatcaa ttctatcgtc cagtgaagca acaaacatct attcgtttag
      481 atgctgacgt acttgcatgg ttcaaagcac aaggcaaagg ttatcagaca cgtatgaatg
      541 aaatcttacg ggatgctatg ctaaaagaat taaaaaatca tcaataaaaa tgggagctta
      601 ggctcccatt tttgactgcg tataacgtgt attatattaa ttttagatat ttttaataat
      661 aagcaatctc taaattgaga tgtttaagtt tatttgattt cgaactaacc ataagagtta
      721 ttcatatcaa tgcttaactt gataagacta aggcttataa atatagtaga gcattgcttg
      781 atttcaagat tttaaataaa ggtattgctg tgattaaagt tatttggggc actaataaag
      841 aaaaaccaat tgcttcaaga caacttgcgg aaacattatc ttctaacatg agtttagaag
      901 gctttcttta tataggttat ccaattgtag gctctcctct aggaccgaca aaatttgatg
      961 cactactttt aagcgagtca aaagggatca ttgcttttga tttagtggaa ggaacggacc
     1021 ttacaaacta tataaataat caagatgaaa tggcctccat gttggaggtg aaattaaaac
     1081 cttatcccaa gttaaaaaaa ggtcgtcatc ttaagtttga tattaaaacc gtaactttct
     1141 gtcccgctaa aaaaaataat ttgccacaag tcgaggataa tctatacaac atcgctaata
     1201 tttccaatct aaatgaagtt atcaattcat ttgagtggga atcagatgaa gatacgttta
     1261 aagaattaaa agcagcaatt caagttgtta ctaatattcg atctggtgct attagaagac
     1321 aaactaaaaa ggaaaattct ttaggaagtc gcctacaaaa attagaagat tctatagcga
     1381 acctagatca gcaccagtct gaagcagtca ttgaaacagt ggatggtgta caacggatca
     1441 gagggttagc aggatcagga aaaactatcg tattagcgtt aaaagttgct tatttacatg
     1501 cacagaacca aagttggaaa atagcagtaa ctttccatac acgtgcgtta aaagaacagt
     1561 ttaaaaggct aatcaacaac tttactattg aacaattagg tagcgaacct gattgggatt
     1621 ctattgatat atttaactca tggggagcac ccggagataa agaaaacgat gggatgtatc
     1681 accgatattg tgttgaaaac tcaatagatt actatgattt ccaaacggct aaaaatgagt
     1741 tcggagggga tgatgctttc aaaaatgttt gtaaactagc tttagaacag tcctcatcta
     1801 ataaaataga aaaatatgat ttaattttga ttgatgaagc tcaagacttc cctcctgagt
     1861 tcattaagtt atgttataaa tttttaaagg aacctaagcg attagtttat gcatatgatg
     1921 aacttcagtc tttaaatggt ctgtccgttt tacctcctga acagttattt gggaaagatt
     1981 ccaaaggagt gccattagta accttagaag aagaagatcc aacaaaacct aaaaaagata
     2041 ttattctcga aaaatgttac cgcaattcac gtccactact tgcaaccgct catacactag
     2101 ggtttggatt aactagacca aaaggattaa ttcaattttt tgataatgat tctctatggg
     2161 aagatgttgg ttattcggtt aaaaaaggta aaattgaagg tggaaaagaa gtagagttaa
     2221 ttagaactag tgaatcaagc cctctatttt tggaagaaca tacacctctt gaagaactta
     2281 taaaatttga ggtatttgat tcagttcgag atatgaatca aatgcttttc gattctattg
     2341 tcaaaaatat ccatgaagat gagcttaagc ctgaagatat cttagttatt aatcctgacc
     2401 ctttcacaac acagaaaaac gtagggattg taagaaaagc attacaacaa aatgagatag
     2461 attctgaaat tgcaggtgtc acaacctcca gagatatctt cagaaaaaat ggatctgtta
     2521 cttttacagg tatcttcaga gcaaaaggta atgaggctgc aatggtctac attatccacg
     2581 ctcaagattg tgcagattcc tatgatccaa atcacttagc actaatcaga aatagattat
     2641 ttactgctat tactcggagt aaagcttggg ttcgtgtctt gggaattggg ccatctatgc
     2701 aggctctaaa agaagaatgg gaaactctaa aaaacaacga ttatgcttta aagtttgttt
     2761 atccgacaga aaagcagaaa aatacattaa agttaattaa taaagatatg tctgttcaag
     2821 agaggaatag acgaagaaaa ctgacacata atattcaaga aattatacat gctatcaatt
     2881 caggagaatt accaactgaa ttaattcctg aagaattaat taatatttta aaaaaatcag
     2941 ggcattaaga tggattctga tgctatttct aaacaatgta ctgaaatcat tgaccgcctt
     3001 attttatctg ggttatcaat agaacaaagc tatccatcca cacgtatgaa tgcgagtgga
     3061 gattatgaaa ttggatttaa aaaaggtatt gaaatttctc aaacgctaaa aaatatccct
     3121 tatgaagata tatataagtc tgttttagat gaaaaaagct atcatattca cctgatagat
     3181 ggaggcttag tttcttttaa ttatattttt gagaaaaatg atgatgtttt tttataaaaa
     3241 acatagattg tcattctatc cttcaccatc attgccatcc tttgaagatt gcccagaact
     3301 ttatagtaat gatgaaattt tcttagagtg tactaaaaaa aatttagtta aatttcccat
     3361 tagattcgac tttgatccca aaaatcatgt gaatgttact catcctgcat cgcatgtaac
     3421 atttgggcaa tttctcaatt gtagaatacc tgtttcaatg ccagttactc caagaaaatt
     3481 tattctattt ttacttcgta atttttatta ttctggctat acaaaaagta agaatatttt
     3541 tgataaaaaa atggttaatg tcattccatt tcatactatt actagtgaag agagaaaaat
     3601 tggtcacttc attttatagt tcacctatac tttatttaac ataaaatctc ttatatgcaa
     3661 ttcagttgta agcccaaatg gaaatgtccg atttctccaa agtaaaaaag cgcctgatgg
     3721 cgctttcctt taatcatatt tatggcgtca gaataacttt acgacagtct tcttcttttt
     3781 tatcaaaaat acggtaacct tccgcagcat cttccagttt catgcgatgg gtaataatca
     3841 gatctggaga cagatctcca ttttcaatat gttctagcaa ctgcggcaag taattatgca
     3901 catgagtttg tcccatttta aaggttaaac ctttgtcaaa ggcatcacca aacaggaagc
     3961 cgtgaattgg accggcataa attccaggta cactcaccac accgccgcgg cggaccgctg
     4021 caatacattg tcttaacgct gaaccactcg aaccttctaa tttcaggtta gtcattacgg
     4081 tttccagcat gctgcctttg gcttcaaaac cgaccgcatc aatgacggca tcgactccac
     4141 gataacccgt ggtattttga atgataaatt cagcggcatc gacttcatca aaattgaccg
     4201 gaatcacccc ataggtttga tgtgcgaaac tcagacgata aggatggtga tcgaccataa
     4261 aaatctgctc ggcaccgagc atccgcgcac aggcagcagc cagcagacca actgggccag
     4321 caccataaat cgccacactg gaacctcgac caacttgggc attgttcact gcttgccagg
     4381 cggttggtag aatatcggtc aggaataata ctttttcatc aggcagggag cctggcactt
     4441 taaatggtcc gacattgccc ttggggatcc gcacatattc agcttgacca cccggtatac
     4501 caccatatag atggctaaag ccaaataaag ctgcccctgg tggaatctgt tttttattga
     4561 gaattgcacc acggccggta ttggtatttt cacaggcagc catgagttca tgttcacaga
     4621 aaaaacagtg accgcatgca atcacaaagg gaataatcac ccggtcacct tttttgactt
     4681 cggttacttc agggccgact tcttctacaa ttcccataaa ttcatggccg aaaatatcac
     4741 cgtcttcagt ggccgggatt ttcccccggt ataaatgcag atctgagcca cagatggcgg
     4801 tggctgtcac ccttaaaata acgtcatctg gttcctgaat cactggatct ggaacggatt
     4861 caactcgtac atcccgagca ccatgatagg taagagcacg catgcggatt tcctctttct
     4921 agttcaacat taaacagact cgtatcaagg ctgccaaaaa taaaaaacac ttttattaga
     4981 gacttatgcg ccaagaaatc gctgtaaatt aatgtatgaa ataagacatc cgagtgttta
     5041 aaaaataaat ttatataggg tttacgtaat tcgtgatctt atgtgtcgaa tgaatagaaa
     5101 agaacagaat taaagaaaca cctagattgt tagaacccat ttctagctat cttataggcc
     5161 taatgcagta gtttccgctg ccatatcttc gcttaagtac tatatgtata taccctgttg
     5221 tagaaggttt aagaggatcc aataatctaa ttttagaagg tccgggattg taacgagcta
     5281 ctgctttcgg atcttcaact tttaaacgct ctacttcttc attgataaca tcacgggcca
     5341 catattttaa aacgctataa gcctctcctt tattcagaaa tttgaaagag gaacttccat
     5401 tttcctttac tcctaaacgg aagtgcttat ctaaaaattt agctaaatca tcagcagaac
     5461 ctaaaaaagg ttttggcata atcttctcct taaactaatc cttatattat tcagcattag
     5521 aactgcaaac tttattaagt atcaatccat taacagtttt aatttaaata agatattgat
     5581 taatcaattg attataattt tcttcatgca cattttcctt accaaactct cgtcctgttt
     5641 cttcaaaaag acgtcttaaa atcacgaatt gaataaaatc ttccgcataa ttttcttgtc
     5701 taggcaaata cgataacgtc aacggctgat aatattcagc gttcctcaaa ttcgattgtc
     5761 ctttcttgta gtgatagccg acagcttgcg tgaacagtcg cccgacttga tgacgattac
     5821 ctttgaacac agtgtaatgt tgtaaatcgg catttaactg ctgccatttc ccgatgccta
     5881 ctgatttatt agggttagaa atcaaatcat taataatttc tttgtatttc ttctcgatta
     5941 aatgaacggt tggttcgtca taccgccatg tccaagtgta gtttttctca ctctttttac
     6001 gatttgaagc atattttttt gagtttttgg tcgagtgtga aagtcgaact agctcgacat
     6061 acttaccatt gccaaaattg agctttaaat agggtttttg aagtacaaat ttgaactttt
     6121 cctgatctcg aaagtagtcc tggatgacgc ttatttcttg ttgttcattc tcacgattcc
     6181 aaacaacatt ttcagcctca gctactcgtt gtccagatag acgtggtttc aagtttattt
     6241 ggctgagctg cgtgttaaat ttatgagtat taggcgtagt aataagaagc caaaaatcaa
     6301 aaatcgtact gtttacacgt ttttgcacaa tgagatcggc aataggctct tttttagtgt
     6361 tcagtcggta tgatctctgc ctcgaagtta ggtctaagtg gtatttatcg gttaatttct
     6421 taatcagctt atctatttta gggaaaaaaa ggttgtcttc tccctcaaca gaaaaatgag
     6481 aataaaaata agcgccatgc ccgaccttat ctacgatttt ctgagctgta taacgactca
     6541 ttgggctgta gtgaatcatt ttattgtcac caagcaatat ttataaaatt gtcatcaaac
     6601 aatattacaa aaacttgatt gtaatcaagc aatatttata aaaagttaaa gacttatttt
     6661 ataagatttt tataaaaatt tagtggcgaa gcgacttttg ccaatgccga aggcattggc
     6721 cggacttaat tgacacaatt tgtcattgtg caattaagtg gttcctaagt gacaatattt
     6781 acacctgttt ttacctgcta tatgatggaa ttattcattg actgaagcag tctgaccaac
     6841 tgcctgacca tttcgttttt acgattttga gcaggcgtca gcctgcttaa agccatgttt
     6901 ttttagatag cgcacgttcc tctactagcc attttgggat gaccgattga gcgtactacc
     6961 tctgaagggg ggttcggtac gatctgatgg gcgctggagt ttactctttc gatccaatcc
     7021 aagctgtgat ttttctttat gacttttcac ggggttgggc gacaaggaca cgggagccta
     7081 taagcaatcg aaagattgtg aaatggtcgt taggttagat atagcctagc tagatggttg
     7141 tttacatcga aaaccctgtt ctagcaatta ccgtgagaac ctgacaggac gttaaggttc
     7201 gttaataacg gttatgtatt gcggctcatg cacgttggaa acaacggata ggcgcagttc
     7261 ataggcacaa ctgtaggcgt ttgaggtaac agcgatgtta tcggctgcgg agaggaaact
     7321 tggaagcagc ggcaccactg agtgagatag tctcaagcaa ggtgggtcaa cggatagctc
     7381 aagcagtata catcgggatg catgaaataa ttccataatt gcccgagata cgtgtagatc
     7441 aagagccata cgctcagatt ttttctgatt tgccatatcc ccattttttt aaggaaccgc
     7501 gttgggggga aactggaagt gcaggtcagg agagagagca ttggcggtct ggtcgttaag
     7561 attgctagaa ataacttggg aatctcttag ctgctgctgt catggcactg gtaggttaag
     7621 agagggagag acgatgagta gggcgtataa ggcatataac ttgttatatg ttgaagtcgt
     7681 ggcaaatatt gctaaaacaa taggcgaact caattgcgac tggcagtcgt aattggtaaa
     7741 gggagtctaa actctatgtg atgtttacgt ggagccgttt ccgaaagttt gagtcggttt
     7801 tatgttgtta tctaaaaaag cgaaaaactc gttaaagatg gtttggtcgc catcgttgtt
     7861 ttgcttgggt caggattggt ctcctgactt atgcaagact aaacaacagt ctaatgaaag
     7921 aatgtgatgt gatatttagc cacttttaga gtgcaacagt cttgttaatc accgctctgt
     7981 tcaatagtgc caggtgctat ttctctggat agttttaaag tttagaaaaa ctgatgttag
     8041 cagacattcc aatctgtgag tttttactct tttttagtaa agaaagggcg tactgtgatg
     8101 attcttaggc tctgccgacc taagttattc gataaacagc gtggtagtta cgtcagtgca
     8161 ctatagcccc ctcggttttc ttcggcaaag aaagcgaaac gaggtgggtg tctttaattt
     8221 ctaaaatgcg atattttgcg attttaggag ctttctaact taggacatag aatctatgct
     8281 taaacgtagt aaatgagcta aaacccccat tttttgcgtt taaaagccat tctcagaacg
     8341 agtgtctacg agtgaacatt gaaaagttgc gcctagactt cctgaaaaac agttttttca
     8401 gtatataaat ttctaaaaac agaaaattat gtctccaaag tctacttcaa atcgagcgta
     8461 gcgagcaaaa aaagctcatg agcgaagcga attccgagtt gcttttgctt tttctaaaat
     8521 tcacgaaaat attaactaaa aaattgcccc gacgaatcga gcgagagcga gattcaatag
     8581 agtttgagcg tagcgaaaac caagggcaat ttttcattcc ctgtgttttt aattatttta
     8641 aggtttttaa atgtttttaa cttattttta attattaaat ttcaataact aatctttgta
     8701 ttcccccacg tttagttacc cattccccca cgtttagtta cccattcccc cacgtttagt
     8761 tacccattcc cccacgttta gttacccatt cccccacgtt tagttaccct cctaattaca
     8821 cccttaacta cacagttgta gttataccca cattgttata atctgtgggt ataaaaatat
     8881 ttcaatcgag tctatgtcaa aagaattagt tgtaaaaacc aatcggttaa accaagcttt
     8941 tcaaactcta tctttatcag agtttcatat tgttcaatta gccattgttg atgctagaca
     9001 tacgggtact ggactaagta cagatactcc tttacgaata gatgctttga gatatgctga
     9061 agtttttggg acaacaaggc aaaatgctta tcagagaatg aaagaagcag aggattcttt
     9121 atttaacaga agatttagct tctttgatga agatgggaaa ttagtcaaaa gtagatggat
     9181 tcagcaagta aaatatctag atgatgaagg agctattgaa cttgttttca ctttagctgt
     9241 tgtacaaggc ataagtaaaa ttgatggtat taaagacttt tttactcaat atttactaag
     9301 ccaaacagcc cagcttaaca gtacatattc agcaagatta tacgagttat taattcaatg
     9361 gagagctatt ggaaaaacgc cagtatttga attagcaact tttagagaac aacttggaat
     9421 tggtgttaat gaatacaagc gtatggatca ctttaaaact agagttcttg acttagccat
     9481 ttctgaaatt agcgaaaaaa cagatataga ggcaacctac caacagcata aaaaaggccg
     9541 ttcaatatct ggtttttctt tttcttttaa acaaaaaaaa tctaaaacta aatcattaga
     9601 aaatcagaca atatctggga atctagatct tttttcaaaa aaaatgactg actctcaacg
     9661 ccacttattt tccaataagc tatctgaact tcctgaaatg agtaaatact ctcaaggaac
     9721 tgaaagctat cctcaattcg cagtaagaat tgctgaaatg cttaaagatt ctgagaagtt
     9781 aaaagagttt gctccaatgc ttgaaaaggt tggctatcgt taaatcattg atgtaacgtt
     9841 acatcactaa atactgtcta ctgacataca gacttttact tgggttacat gatgaaagca
     9901 ctatctgtta ttgaattagc tcaactctat ggaatgaatc gtcaaagcat ctataaacgt
     9961 ataaataaag gagatttatc aaaaaatagt gatggaaaaa tagatttttc agaagctcta
    10021 cgtgtatttg gtgaaccatc ccacaataat aaggatgtaa ccacgttaca gtctacttcg
    10081 gtacaaaaag agacagaagt agacatgctt aaacaacaag tagacatact aaaaaaacag
    10141 ttagaacttg cacacgaacg agaagttttt caacgagaac aactagaagc aaaagacaat
    10201 caaatagaag caatacaacg actgttagaa gctccaaaaa ccaatatgac tacgtttacc
    10261 gatcagaaac ctagccaaga tatagcaacg gatcctcggt cagaacctga gccgaaacat
    10321 gacggattga ctactccgga acaaacagaa aataagcgga ttccggtgcc ggatcacgtt
    10381 gaaccggagc agccgaaaag aggcttttgg agccgttttt ttagacccta tgattaacca
    10441 cgattgttta actaatatga ataaagtcgt gaaatattga tcaaataata ggcgtggaac
    10501 gtgaaaaatc ataaaaaaaa agcccacctt ggggaggttg ggcaaaaaga aactacagcg
    10561 ttgaattttc aagagaatta taacgcattt cgtataaggt gtattatgtt aattttagga
    10621 aatcttaagc tttagccaac ataatcttca gttgatatta cttgagcata tgcaaattca
    10681 aaagcggcca ttaatgttgc atggacatga ccagcaggta ctttaacgcc attaaattcc
    10741 aagtcagctc tggccctaga atcatttttt taacttttca taacgaagaa taaaaataac
    10801 tcaatgttta ctttaggttt tatacgcttt ttcttttcag gagataatgc taggatcaaa
    10861 attggctaac attaaataat cataatgaag aatcttcaaa tagttatagc acaagctcta
    10921 cattcatatg aatttgaaca tatggagcaa tcaataagta aatccattag cgacactata
    10981 ttggaaatat tggtcaatga tgggcctttt gaaaaaattg attgttatct gtattcaaaa
    11041 aaagattcta aaaaaattct aaaaagctct atacactatg taattgctgt cttagaaagg
    11101 gcgaagtata gagtacagaa agctcagcaa gataatctga gtcctttgta tatctgctta
    11161 tacgtgatta agaaaattga gggctttatc gaatacttaa atactaaatt ttaactgtga
    11221 aagcctccat ttaacataat ggcgattata cgaagtacac ttgtatatta atataaatat
    11281 cagatactta aaattttggc tctagccaaa aaatgcacaa aaagccgatt ttgaaacaag
    11341 tcggcttttt tatgagcagt tttgaaatat tttgccaata aatttgacta aaaattacgc
    11401 ttttcagctt gagtgataca attttgcgat acattattgt atatacaaat ttgaatgttt
    11461 tttat
//
