LOCUS       NC_025003               5933 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Enterobacter cloacae plasmid pCY-MdT, complete sequence.
ACCESSION   NC_025003
VERSION     NC_025003.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226756
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Yang,L., Wu,A.W., Su,D.H., Lin,Y.P., Chen,D.Q. and Qiu,Y.R.
  TITLE     resistome analysis of Enterobacter cloacae CY01, an extensively
            drug-resistant strain producing VIM-1 metallo-beta-lactamase from
            China
  JOURNAL   Antimicrob Agents Chemother 58 (10), 6328-6330 (2014)
   PUBMED   25114139
  REMARK    Erratum:[Antimicrob Agents Chemother. 2016 Jan 29;60(2):1176. doi:
            10.1128/AAC.03100-15. PMID: 26826195]
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Yang,L., Wu,A.-W., Lin,Y.-P., Chen,D.-Q. and Qiu,Y.-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2013) Department of Laboratory Medicine, Nanfang
            Hospital, Southern Medical University, Guangzhou, Guangdong 510515,
            China
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009962415.2-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 01:08:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8
            CDSs (total)                      :: 8
            Genes (coding)                    :: 7
            CDSs (with protein)               :: 7
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KF998105.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="CY01"
                     /db_xref="taxon:550"
                     /plasmid="pCY-MdT"
                     /geo_loc_name="China"
     gene            complement(160..375)
                     /locus_tag="HTC04_RS00005"
                     /old_locus_tag="D727_p5002"
     CDS             complement(160..375)
                     /locus_tag="HTC04_RS00005"
                     /old_locus_tag="D727_p5002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137421.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(379..864)
                     /locus_tag="HTC04_RS00010"
                     /old_locus_tag="D727_p5003"
     CDS             complement(379..864)
                     /locus_tag="HTC04_RS00010"
                     /old_locus_tag="D727_p5003"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015059929.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSTLLTASGASILWWQGQQIL
                     DNYTIIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<899..1603)
                     /locus_tag="HTC04_RS00050"
                     /pseudo=""
     CDS             complement(<899..1603)
                     /locus_tag="HTC04_RS00050"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1542..1865)
                     /locus_tag="HTC04_RS00020"
                     /old_locus_tag="D727_p5004"
     CDS             complement(1542..1865)
                     /locus_tag="HTC04_RS00020"
                     /old_locus_tag="D727_p5004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3254..3547
                     /locus_tag="HTC04_RS00025"
     CDS             3254..3547
                     /locus_tag="HTC04_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052905524.1"
                     /translation="MTIVISKEIEDKLTNKHKVTIKEVHESLANFKGNLLIDNREQHRT
                     NPPTNWFIAETNQRRELKVCFMIIDGDIHIKTAFEPNELERKIYAKHGKKTA"
                     /name="hypothetical protein"
     gene            3745..4113
                     /locus_tag="HTC04_RS00030"
                     /old_locus_tag="D727_p5008"
     CDS             3745..4113
                     /locus_tag="HTC04_RS00030"
                     /old_locus_tag="D727_p5008"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059931.1"
                     /translation="MKTTSEFARDAQKWDDRELGASEDHVEVASMAEMSAFNDALSLQA
                     ISIRLQKNLVSDLKSIAESYGIGYQPMVRDLLQRFVIAEKKQALRRELDKLNEREEMHQ
                     QEDTVPVDQFIESIRKQA"
                     /name="hypothetical protein"
     gene            4295..4849
                     /gene="aac(6')-Ib"
                     /locus_tag="HTC04_RS00035"
                     /old_locus_tag="D727_p5009"
     ARG             4296..4846
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            5343..5894
                     /locus_tag="HTC04_RS00040"
                     /old_locus_tag="D727_p5010"
     CDS             5343..5894
                     /locus_tag="HTC04_RS00040"
                     /old_locus_tag="D727_p5010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000654246.1"
                     /translation="MKDMKAVVVFTGKDLNIMRTEGGSGYWHARTDRLNDADYLIAVRN
                     RRETWAVKDLEHGTAFLIAKITGCFKSPDYDDRNVITFDEYAEIHTPKAWKMLTDGQRY
                     PVAYLSAQEAFLRIGVTPEQLEWKKFHPSSPSVPNTVIPGLAEEKTEKLSLNEAIERAK
                     KDISNATGIDSSAITISIKI"
                     /name="hypothetical protein"
     ARG             4173..4849
                     /name="AAC(6')-Ib8"
                     /gene="AAC(6')-Ib8"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(1967..2153)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.34"
                     /coverage="87.0"
                     /evalue="2.7100000000000003e-47"
     Typing          complement(57..1552)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.385"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actgaacctg tacgccagaa acagcaagcc catgcttttc cggcgcacag attcaaagtg
       61 acggaccgtt gtacgttcgc tgtcgctgag atcgttccag ctccagcgct ttctgatgct
      121 gcgccgcccg ttcctgctca cgatgtgcct ctatcacgtt cagccttgtg acaaccggcg
      181 cagctgctcg ctcaaactct gcacctgcct gctcaagccc gtgacgcgct cgctcagcgc
      241 cgcgttctcc cgttgcataa gaccagacat cgtccgccat tccgcgaagg cgttctccca
      301 ctcgtccagc cttttcgagt agtcctgctg tagctgctct aatgcgctca gcaactgttt
      361 ttccagctcc gtcatgtgct atttccccgc cagtatcatc cagctcgaat cctctccgaa
      421 ccgtcccgct tccgggttca ccctcacgca gcggcgtctc tgctctccgc aggtcgagag
      481 ctgcacgccg ctgttcctct cggacagcat ggcctgcgtg ctcttctgct cccggatgat
      541 cgtgtaattg tcgagtatct gctgcccctg ccaccataga atgcttgcgc ccgatgctgt
      601 cagcagtgta gacaccagga cgatggtcag ccacgtctgg ctgaccatac gcaccatgcc
      661 tttcgtgcgc tggctcatgg ctgaggacag cttccggtcg tggtcgagga tggcggcgct
      721 gattctcttc tcgctttcgc tcagttccgc tctgacagac ttctcgtgct cgctgaatgc
      781 ggctttcagc atctcgccgg tactctgctg ctgcgctttc gatttctgct ctaagtcctt
      841 tgccagcgtt aaaagactgt tcatagatgg ctcccttcag tcggatgttt cgccccccgt
      901 ccgggtcggc aatgctgatg ctgctttttg tggtgcgtac cacctcaaaa cctgcgcttt
      961 ccagcgcctc agtgacgtcc tggcgggttt tcagctcccc ggacgaggct aaggccagta
     1021 aaccccgtgt aattgcctga gcggcttcct gcttcgtttt cggcagcgcg gacggcgtga
     1081 ccagcgcccg ccggttttca ggcgcgttcg ggtcatgcag ccccagcctg ccgttcacga
     1141 tggtctgcca ggcatcaatg cgcggacggt cggcgcggtc gtaatacggc tggagacgcc
     1201 tgccagtcag cagctccatg ttcgggatca ggaagttcag ctccagccgc cccttatcct
     1261 ggtgctcaac ccacagcacg ctgtactggt ctttatcgag tccgggcatc agaacccgtt
     1321 caaagctcgc catcagcttt tcgcgctgcc cgggcggtaa atccttttcg gcaaaggaca
     1381 ggacgccgga tgtgtacttt tttgcgtagg gcgaggcgtc gataagctcc ctgacttctt
     1441 ccggcttccc ctgcaaaacg gtggcgccgt cgcgctgtcg gtctttcccc agcagataat
     1501 ccaccggacc ggcgccaccg ccgcgccctc gcgggtgaaa cttaacgatc atcgtctgca
     1561 cccttttcca gcacggcatg ccgcagccgc tcgagtccgg catctatggc catcagcgcg
     1621 gcgacgacct gcacgcggtc gtgcccgctg ccaccaccgg cgttaacctg ccgggcaatc
     1681 tggttgaggt tgttccccat gccggcaagc tgacgaagca gcgccggcga gatcgaggga
     1741 agcttgccgg cgcgggcggg tttctcgtcc aagcacgtct ggcgcatcca ggctgcaagc
     1801 tgtttaccgt cgcagcgttc caacagacgg cggtgctcat cttcggtcac ccacatcgtg
     1861 agcattttac tgcgtttgtc tgccaatcca gcctcctgat aacgccggga cgggcgggaa
     1921 atcttttccc ggtcggcgcg gtgttggaca agccgcaggc gcgtcagtgg ttttagcagg
     1981 tttcggggcg cagccctgaa ccagtcacct agcgctagcg gagtgtatac tggcttagta
     2041 tgttacggcg agatggagat tcaggaaagt gcttcatgtg agagggggaa aatgctgctg
     2101 cagggagtag gtgcgaaatg ccggtgtcgg tgacatgacg cctcctcgct cactcggtcg
     2161 ctacgctcct gccgtgagac tgcggcgggc gttacgggct tacagataac gggatagcaa
     2221 gaagtcagga aaggcggaaa aaggatgcgg cgttgccgtt tttccatagg cttcgccctc
     2281 ctgacgagct tcacggaaat cagacgatgg ccggtgctca aatcagaagg ggcgaaacct
     2341 gacaggactt aaagatcccc accgtttccg atgggtcgct ccctcgtgcg ctctcctgtt
     2401 cccaccgtga ggtttacccg ttgcagaccg ccgtaaggtg gcgttttctc attccctccc
     2461 atgcaactgg gttcctgtta ggtggttcgc tccaagctgg actatatgca cgaactcccc
     2521 gtttagtccg actaccacgt ccgatcaggt aactgttctc ttgattccaa ccctgacgga
     2581 cacgacaaat cgccactggc ggtagccaac ggtaactgaa ttacggagcg ggaaagtaca
     2641 caatagaaaa atcagagttc ttgaagtgtc accataacta cggctacact ggaaggacag
     2701 ttttggttac tgcgctccgt taaagccagt taccacggtt aaaagatctg ccaggatcat
     2761 ttaaccttcg aaaaaccacc tgccagggtg gttttttcgt tttggataga agagattacg
     2821 acgactaaca tatgatctca agaagatcct caacttaaga ctgtgccttt ttccttttgc
     2881 tatctgcagc gcctttagcg cgacagcctc tacaccacgt ctgaggggct acgttaccgc
     2941 gtaaatttct aaagccaaat agagtttcaa cttcttgata ggtcgaagcc acgactgggt
     3001 ttaatcgaca atcatagtca gcacaatgac atggatgagg caacgctatt gtattgctca
     3061 taatttctcc tatatctatc tttcataacc aacagcaata ggccaaaagc agagaaaacg
     3121 cagtttcctc tcagtcgctg tatatacaaa aaaacagaca ataaccctac caacgctgga
     3181 caaaattgac aagtgtaaat tttgtatata caataaatcc aatttcacaa caaaggccgg
     3241 ggcatcatgg aatatgacaa ttgtaatcag taaagaaatt gaagataaac taaccaataa
     3301 acacaaggta acgataaaag aagtacatga aagccttgcg aattttaaag gcaacttgtt
     3361 gatagataac agggagcagc accggactaa tcctccaact aactggttta tcgctgaaac
     3421 taaccagcgt agagaattaa aagtgtgttt catgattatc gatggcgaca ttcatatcaa
     3481 aacagctttc gagccaaacg aattagaacg taaaatctat gctaagcatg gaaagaagac
     3541 ggcataacag cgctaaataa gttattattt tgcaagggct tacatgataa gcccttgcaa
     3601 aaagcttacc tcagcgaaaa aaaacaaaac taaaatgaag ctgaacctga tttctcaaca
     3661 tagaagttcc tttcggaact agatcaagca taaaaattgc agctaatcct gatctgcagg
     3721 aaacagcacc tagaggtcta tacaatgaaa actaccagtg aattcgcacg tgatgcacag
     3781 aaatgggatg atcgagagct cggcgcctct gaggatcacg ttgaagtagc atcaatggct
     3841 gaaatgtcag ctttcaacga tgcattatca ctgcaagcca tctcaataag attacagaaa
     3901 aatcttgtaa gcgatttaaa atcgatagct gaaagctatg gtatcggtta tcaaccaatg
     3961 gttagagatc ttctgcaaag atttgttatt gcagaaaaaa aacaagcatt acgtagagag
     4021 ttagacaagc ttaatgaacg agaagaaatg catcagcagg aggacacggt tcctgttgat
     4081 cagttcattg aaagcataag aaaacaagca taagaaaact taccatctag atacaaagaa
     4141 tctcatcatc acgtttaaaa ggaggattac catgagcctt aaacccggac caaaaagaat
     4201 tgccgaatcg acggggcaac ctgatcaacg ccaacgcgac aataaaaaga cgcctggaaa
     4261 tactgacaag ttaggcatca caaagtacag catcgtgacc aacagcaccg attccgtcac
     4321 actgcgcctc atgactgagc atgaccttgc gatgctctat gagtggctaa atcgatctca
     4381 tatcgtcgag tggtggggcg gagaagaagc acgcccgaca cttgctgacg tacaggaaca
     4441 gtacttgcca agcgttttag cgcaagagtc cgtcactcca tacattgcaa tgctgaatgg
     4501 agagccgatt gggtatgccc agtcgtacgt tgctcttgga agcggggacg gatggtggga
     4561 agaagaaacc gatccaggag tacgcggaat agaccagtca ctggcgaatg catcacaact
     4621 gggcaaaggc ttgggaacca agctggttcg agctctggtt gagttgctgt tcaatgatcc
     4681 cgaggtcacc aagatccaaa cggacccgtc gccgagcaac ttgcgagcga tccgatgcta
     4741 cgagaaagcg gggtttgaga ggcaaggtac cgtaaccacc ccagatggtc cagccgtgta
     4801 catggttcaa acacgccagg cattcgagcg aacacgcagt gatgcctaac ccttccatcg
     4861 agggggacgt ccaagggctg gcgcccttgg ccgcccctca tgtcaaacgt tgaaacccag
     4921 taaatcttca ggaaagtaag ttgtcagcaa agcccttcgg ggcttttttt atacctgcag
     4981 gtcggcatga tttaccataa caccccttgc ccgcaccgct ccggacgggt tcagcgtgaa
     5041 agcggcgtta gcccggtaaa accggcagta aactccccgc taaaccgcgc attctgcgcc
     5101 tgataaattt cacgcgactg catagccatg cagccacgtg aatggctcat gctttcaatg
     5161 tatatcctcg tccaagcccc tgttttaacc ggtttatcaa aaaatcaaac agaaagcctt
     5221 accactaaca gtaaaagagg tagggggctg tattttatcg cataaaaaac atttacaaca
     5281 cgaattctat cgtatagatt tcataaaaaa atgaaattta ctcacctatt aagggtttct
     5341 atatgaaaga tatgaaagct gttgtggttt ttaccgggaa ggatttgaac ataatgagaa
     5401 ctgagggggg gtcaggctac tggcatgcca gaacagacag gctcaacgat gccgattacc
     5461 tcatagcagt aagaaacaga agggaaacgt gggcagtaaa agacctagaa cacggcacag
     5521 cattcctgat cgccaaaatt accgggtgtt ttaaaagccc tgattacgac gacaggaacg
     5581 tcataacctt tgatgaatac gcagaaattc acacgccaaa agcatggaaa atgctaaccg
     5641 atggtcagcg ctatccggtt gcttacctga gcgcacagga agctttttta cgtattgggg
     5701 taacaccgga acaactggaa tggaagaaat tccatccttc atccccatcg gttcctaata
     5761 cagtgattcc tggtctggct gaagagaaaa cagaaaaact ctcccttaac gaggcaattg
     5821 aaagagccaa aaaggatatt agcaacgcaa cagggattga tagctcagcc ataacaataa
     5881 gcatcaagat ctgatgaagc gtgaacaaca agatagcagc ctcatgttct aga
//
