LOCUS       NC_023328               6822 bp    DNA     circular CON 26-APR-2024
DEFINITION  Escherichia coli ACN001 plasmid pACN001-E, complete sequence.
ACCESSION   NC_023328
VERSION     NC_023328.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225949
KEYWORDS    RefSeq.
SOURCE      Escherichia coli ACN001
  ORGANISM  Escherichia coli ACN001
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Wang,X., Hao,H., Xu,Z., Zheng,H., Liu,C., Wei,L., Zhang,R., Bi,D.,
            Chen,H. and Tan,C.
  TITLE     Plasmid-mediated multidrug resistance and virulence in an avian
            pathogenic Escherichia coli strain isolated in China
  JOURNAL   J Glob Antimicrob Resist 2 (1), 57-58 (2014)
   PUBMED   27873640
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   Wang,X., Hao,H., Xu,Z., Zheng,H., Liu,C., Wei,L., Zhang,R., Bi,D.,
            Chen,H. and Tan,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2013) College of Veterinary Medicine, State Key
            Laboratory of Agricultural Microbiology, Huazhong Agricultural
            University, No.1 Shizishan Str. Hongshan District, Wuhan, Hubei
            430070, China
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Newbler v. V2.3
            Sequencing Technology             :: 454
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009961225.2-RS_2024_04_26
            Annotation Date                   :: 04/26/2024 03:16:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 13
            CDSs (total)                      :: 13
            Genes (coding)                    :: 12
            CDSs (with protein)               :: 12
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KC853438.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Escherichia coli ACN001"
                     /mol_type="genomic DNA"
                     /strain="ACN001"
                     /db_xref="taxon:1311757"
                     /plasmid="pACN001-E"
     gene            85..273
                     /locus_tag="G7C80_RS00005"
                     /old_locus_tag="J444_pE1"
     CDS             85..273
                     /locus_tag="G7C80_RS00005"
                     /old_locus_tag="J444_pE1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225393024.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTHLCG
                     ISHPAWRTFHTILTDAK"
                     /name="hypothetical protein"
     gene            complement(425..616)
                     /locus_tag="G7C80_RS00010"
     CDS             complement(425..616)
                     /locus_tag="G7C80_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_050863702.1"
                     /translation="MTKQEKTALNMARFIKSQTLTLLEKLNELDADDQADICEALHDRA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            684..1007
                     /locus_tag="G7C80_RS00015"
     CDS             684..1007
                     /locus_tag="G7C80_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1216..>1650
                     /locus_tag="G7C80_RS00065"
                     /pseudo=""
     CDS             1216..>1650
                     /locus_tag="G7C80_RS00065"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1685..2245
                     /locus_tag="G7C80_RS00025"
     CDS             1685..2245
                     /locus_tag="G7C80_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            2252..2485
                     /locus_tag="G7C80_RS00030"
     CDS             2252..2485
                     /locus_tag="G7C80_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2969..3391)
                     /locus_tag="G7C80_RS00035"
     CDS             complement(2969..3391)
                     /locus_tag="G7C80_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_021539380.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTT
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            3513..3791
                     /locus_tag="G7C80_RS00040"
                     /old_locus_tag="J444_pE6"
     CDS             3513..3791
                     /locus_tag="G7C80_RS00040"
                     /old_locus_tag="J444_pE6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(3783..4073)
                     /locus_tag="G7C80_RS00045"
                     /old_locus_tag="J444_pE7"
     CDS             complement(3783..4073)
                     /locus_tag="G7C80_RS00045"
                     /old_locus_tag="J444_pE7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            4318..5790
                     /locus_tag="G7C80_RS00050"
                     /old_locus_tag="J444_pE9"
     CDS             4318..5790
                     /locus_tag="G7C80_RS00050"
                     /old_locus_tag="J444_pE9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000362089.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVGG
                     GGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQRLK
                     DIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALREAE
                     RQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELAKAES
                     DVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSVTNALN
                     SAVSIRDAKKTDVQNAEIKLKEAKDALEKSQVKDSVDTMVGFYQYITEQYGEKYSRIAQ
                     DLAEKAKGSKFSSVDEALAAFEKYKNVLDKKISKVDRDAIFNALESVNYDELSKNLTKI
                     SKSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGV
                     PLGFWGIAIVTGIVSSYIGDDELNKLNELLGI"
                     /name="colicin-like pore-forming protein"
     gene            complement(5787..6077)
                     /gene="cki"
                     /locus_tag="G7C80_RS00055"
                     /old_locus_tag="J444_pE10"
     CDS             complement(5787..6077)
                     /gene="cki"
                     /locus_tag="G7C80_RS00055"
                     /old_locus_tag="J444_pE10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            6107..6238
                     /locus_tag="G7C80_RS00060"
     CDS             6107..6238
                     /locus_tag="G7C80_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_113918746.1"
                     /translation="MKIIVFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6433..6576)
                     /locus_tag="G7C80_RS00070"
     CDS             complement(6433..6576)
                     /locus_tag="G7C80_RS00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     Typing          253..340
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2..242)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.256"
                     /coverage="100.0"
                     /evalue="4.33e-79"
     Typing          complement(6468..6822)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.596"
                     /coverage="87.0"
                     /evalue="1.25e-177"
     Typing          998..2592
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggaaaaag cgcctcctgc gcggcctttt tcggggtttt cgtttcttgc tcacatgttc
       61 tttccggcat tatcccctga ttctgtggat aacactatca ccgcttttga gtgggcagat
      121 accgctcgcc gcagccgaac gaccgagcgt agcgagtcag tgagcgagga agcggaaaag
      181 cgcctggacg agcattttct ccttacgcat ctgtgcggca tttcacaccc ggcatggcgc
      241 acttttcata caattctcac tgatgccaaa tgattaagcc agtatacact ccgctatcgc
      301 tacgtgactg ggtcatggct gcgccccgac acccgctaaa acccgctgac gcgccctgac
      361 gggcttgtct gctcccggca tccgctcaca ggccagctgt gaccgtctcc gggagctgca
      421 tgtgtcagag gttttcaccg tcatccccga agcgtgcgag gcagctgcgg taaagctcgt
      481 cagcgcggtc gtgaagcgct tcgcagatgt cagcctggtc gtcggcgtcc agttcattca
      541 gtttttccag cagggtcagc gtctggcttt tgatgaagcg ggccatgtta agggcggttt
      601 tttcctgttt agtcacagtt ctgtttctcc atgtgtgggg agtctgtcgg aggtttttaa
      661 tgataccggt gaaacgcgaa aggatgctca cgatacgggt cacggatgag gaacatgcgc
      721 ggctgctggc acgttgtgag ggaaaacaac ttgcgtcatg gatgcgtaag gtctgcctgg
      781 gggcaccgcc atcgaaaacg tcaggtcttc cgacactggc accgccgctg ttgcgccagt
      841 tcgcttcggt cggcaataac ctgaatcaga tagcccggaa gataaacagc ggacagtggt
      901 cggggcatga ccgggttcat gtggtggctg cactgatggc catcggacgt gaactgagtg
      961 agctgaggga tgaggtccgt aaacagggag aacgggatga tagttaagtt tcatgccagg
     1021 ggaaaaggcg gtggcagtgg tccggttgat tacctgctgg gccgggaacg tagccgggaa
     1081 ggggcgagag ttctgcgggg tgctcccgaa gaggtgcggg aactcatcga tgccacgccg
     1141 tttgcgaaaa aatacacctc gggggttctg tcttttgcag agcagaccct gccaccggga
     1201 gagcgtgaaa gggtgatgga gagctttgag cgggtactga tgcccggtct tgaaaaaaat
     1261 cagtacagca tcctgtgggt ggagcaccag gacaaggggc gtcttgagct gaattttgtc
     1321 atcccgaata tggagctggc gagcggaaaa cggctccagc cctattacga ccgggcggac
     1381 cgtccgcgaa tcaatgcctg gcagacactg gtgaatcacc attacgggct gcacgacccg
     1441 aacgcaccgg agaacaggcg gacacttgtc accccgaata atctgccgaa agcgaaacag
     1501 gaggccgcag aagcgattac gagggggctg gaggctcttt accatgccgg agcgctaaaa
     1561 acgcgtcagg atgtcactga ggcgcttaca gcggcaggtt ttgaggtggt gaggacaacc
     1621 aggagcagta tcagtatcgc tgatccggag ggcgggcgta atctcaggct gaaaggggcg
     1681 ctgtatgagc aatcttttac aaatggggac agacttcgag aagaaactga aagagcgtgc
     1741 cgtctctaca gagaacatgc tgaacagcga gttcaggaag ctcgaagcgt ctgtagacag
     1801 ggaactgaac ttaaacgggc agaaaatcag agacgctatc agcgcacaca cagcagcggt
     1861 gaaagaacaa ctggagacgc tgaacagtgc cgtgaacgcg cagttcagcg ccacagagaa
     1921 cgaactggcc cggcggcagg aagagctgct ctggaagctg gtcaaagggc ggatactgta
     1981 cccgtcgctg acggcgcttt gcgtcacggg cgggattttt ctggcgtcat gggggctgat
     2041 tcagtggcag gaaagccgga ttgcggcgaa catcctggct atcagggacc aggaagagac
     2101 gctggcgaaa ctccgggaaa aaacatgggg tgtgacgtat cacgagggca ggaacgggaa
     2161 atttctggtg ttgccgtccg gggtgaaggg ggaaaacaac tggacggtgg agaagaagaa
     2221 cgcagtaaga ctggtcaggg agtaagtgag tatgacagag ctggaaatgc acttactgaa
     2281 cgccttagag caattgcagc aggactatac gcagcggctg aaagactggg agagcgcctt
     2341 cacggatttg cagcatatgt tcgagctgac acgacaggag aacgggcaac tgagggaaca
     2401 gtgcgacgac ttgagtcagc aggtgcagag attagccggg caggtggacc gcttgagtcg
     2461 gttatttatc acgaacagca gataagggat gaacaggaaa ggcaggcacg cctggaacgg
     2521 gagcgtgcgc ggtcctggca tgaagagcag gaacgggaag cacgggtatg gcgtggcccg
     2581 tcaatgggga tgtgacggtg agttgttccg ggaggttgcc agatggtacc ggaacgcgtt
     2641 aaaaacgcat ccgggcgcga ttctggcgtg tcgtttttgt tgctgtgttt tgcctctttg
     2701 ccagatcgcg ctcattcctc agcctcatcc cgcgatgtct gcgtatgtca tcaactgaaa
     2761 agatggaaaa aaaacagtgt catgaggttt acgcaggcaa aaccagcgtt attcacatgg
     2821 ctgaatagct atgcatacgg ttaaaattta tcaggtgcga tcgcggcagc ttttcgggtg
     2881 gtttgttgcc atttttacct gtcagcagcc gtgatcgcgc tgaacgcatt tcagcggtgc
     2941 gtacaattaa gggattatgg taaatccctt actgtctgcc ctcgtatcca tcgagataaa
     3001 ccgcagaaat ggtgtctgcc attagtttca tttttttcgg cgaaggcata tcgctgtttg
     3061 tgtattttct gccagcaaag gttgttgtgc tccggagtga gtgaaaaaat ttttctgagt
     3121 ggaattcttt tacacgttgc tgtgccgttt ctgctgatac tccggctgct ttgtctttca
     3181 tccccatatc ccggaactgc tgaaagaacg ggagcatcat acgtatggtg tctgctttct
     3241 gggtgtagaa gttaggatca aatccatcat ctgaagcata aacataatgc cgggtatgtc
     3301 gttccaggct ggtctttggt gtgcatcctg taagagctat tgttgctgtt atcagaggta
     3361 tcagaagcag cctggcagcg gcagatctca taaacatctc cttgcgtttt taatcttgat
     3421 actcagggag ttatatctcc gaagagtatc tcttatcata cgtcggttta ctcccaatgt
     3481 gatttgatgg tcaatgagtc aggaataatc ccgtgaaaaa atgtgtgcca gtcctgcttt
     3541 ttagtgttct ttctgcttcg gttgccagtc atgcggacag caatgttttt caacaaaagg
     3601 ccgagaagac aaaaaacggc attgttgcgg atgttaatgc cagacagaaa atgttcagta
     3661 acagactgaa gatacaactt cagatcgatg agttgagagc agagctggct ggcaccagag
     3721 atgcccaaaa aagagaggat ttgcgtaaaa aactggagtc tctggagcgg caaaagaaat
     3781 ccttacgctg atgggggcgt attttccccg gtttcttcca taaccttttt accgttcaga
     3841 tatttacggg cgaggcgaat gcgtcttgcc tggtataccg ccgagtaagc ctgggtggga
     3901 ttcacaccat tactgatttc actgaccaca tgacgctgat ctgccagtgt cagcgtgtaa
     3961 acctgtttct ggatgaggtc acgggctttg tcatgctcat atccggcagc aacaagggtg
     4021 tcgagaagtc gtttgtctgt tttgttcagt ttatggcggg gtgatttgct cacatcgttc
     4081 tccgtttttt catgatccaa cagttttata tctcatcctg atttgctccg gtgttctgtt
     4141 tcgacgttct caatgcctgc ctccccatag tgtgatcctt attgcaaaaa tgaaaatcca
     4201 tgctcttgac atggacaatg ctgagtagta ggttttcact gtacataaaa ccagtggtta
     4261 tatgtacagt atgttgtttt taatttattg ttttaattat caaagaggaa tttctttatg
     4321 gataaagtca ctgataattc tccagatgtg gagagcacag aatctactga ggggtcattc
     4381 ccaactgttg gggttgatac tggcgatacg attacagcga cgcttgcaac tggaactgaa
     4441 aatgttggtg gaggcggtgg agcatttggt ggggccagtg aaagttctgc tgcgatacat
     4501 gcaaccgcta aatggtctac cgcgcagttg aaaaaacatc aggctgaaca ggctgcccgt
     4561 gctgctgcgg ctgaggcagc attggcaaaa gcgaaatctc agcgtgatgc cctgactcaa
     4621 cgtctcaagg atattgttaa tgacgcttta cgtgctaatg ccgctcgtag tccatcagta
     4681 actgaccttg ctcatgccaa taatatggca atgcaggcag aggctgagcg tttgcgcctt
     4741 gcgaaggcag agcaaaaagc ccgtgaagaa gctgaagcag cagaaaaagc gctccgggaa
     4801 gcagaacgcc aacgtgatga gattgcccgc caacaggctg aaaccgcgca tttgttagca
     4861 atggcggagg cagcagaggc tgagaaaaat cgacaggatt ctcttgatga agagcatcgg
     4921 gctgtggaag tggcagagaa gaagctggct gaggctaaag ctgaactggc gaaggccgaa
     4981 agcgatgtac agagtaagca agcgattgtt tccagagttg caggggagct tgaaaacgct
     5041 caaaaaagtg ttgatgtgaa ggttactgga tttcctggat ggcgtgatgt tcagaaaaaa
     5101 ctggagagac aattgcagga taagaagaat gaatattcgt cagtgacgaa tgctcttaat
     5161 tctgcggtta gcattagaga tgctaaaaaa acagatgttc agaatgctga gataaaatta
     5221 aaagaagcta aggatgctct tgagaagagt caggtaaaag actctgttga tactatggtt
     5281 gggttttatc aatatataac cgaacaatat ggggaaaaat attccagaat agctcaggat
     5341 ttagctgaaa aggcgaaggg tagtaaattt agtagtgttg atgaagctct tgctgcattt
     5401 gaaaagtata aaaatgtact ggataagaaa atcagtaagg ttgatcggga tgctattttt
     5461 aatgcgttag agtctgttaa ttatgatgag ttatctaaga atttaacgaa gatatcaaag
     5521 tcgcttaaaa tcactagtcg agtatctttt ctatatgatg ttggttctga ttttaaaaat
     5581 gcaatagaaa ctggtaactg gcgacctttg tttgtcacgc tggagaaaag tgcggtcgat
     5641 gtaggtgtgg caaaaattgt agcattaatg tttagtttta ttgttggtgt gcctcttggt
     5701 ttctggggga ttgcaattgt cacaggtatt gtttcttctt acatagggga tgatgagttg
     5761 aacaaactta atgagttgtt agggatttaa tttctcttta gagtgtaata tatgaaactt
     5821 ggcaatccta ctgggattgc cattaataaa taaaacaatg cagataactt gcataagtac
     5881 cagggttctt tttttaactt tgtattttct ttgatatatc tagatatggt taatttagag
     5941 tatggatata gcaatacatg tattgatgat ataaatataa ataacaaagg agtgttatca
     6001 ttgtcgttaa atattaaaat aagaatccat ggtataagtg attcaggtaa attatgtagg
     6061 tagtatttta aatgcataag tatttttact tttgttggaa agaaatatga aaataatagt
     6121 ttttctaatt acaatattat tcttgtcagg atgtcaagca aactatattc gtgatgtgca
     6181 aggaggaacc gttgcaccat cttcgtcttc tgaactgacg gggatcgcgg ttcagtagaa
     6241 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cctgtaaacg
     6301 aaaaaaccgc ctggggaggc ggtttagtcg aaggttaagt cagttgggga ctgcttaacc
     6361 gggtaactgg ctttagtgga gcacagatac caaatgctgt ccttccagtg tagccgctat
     6421 aaggcttcaa cttcaagagt tctgcgtatc taaatccact atccagttac caatggctgc
     6481 tgccagtggc gttaagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6541 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6601 ctacaccgaa ctgagatacc aacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6661 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6721 gcttccgggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6781 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ct
//
