LOCUS       NC_023324               5784 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli ACN001 plasmid pACN001-C, complete sequence.
ACCESSION   NC_023324
VERSION     NC_023324.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225951
KEYWORDS    RefSeq.
SOURCE      Escherichia coli ACN001
  ORGANISM  Escherichia coli ACN001
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Wang,X., Hao,H., Xu,Z., Zheng,H., Liu,C., Wei,L., Zhang,R., Bi,D.,
            Chen,H. and Tan,C.
  TITLE     Plasmid-mediated multidrug resistance and virulence in an avian
            pathogenic Escherichia coli strain isolated in China
  JOURNAL   J Glob Antimicrob Resist 2 (1), 57-58 (2014)
   PUBMED   27873640
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Wang,X., Hao,H., Xu,Z., Zheng,H., Liu,C., Wei,L., Zhang,R., Bi,D.,
            Chen,H. and Tan,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2013) College of Veterinary Medicine, State Key
            Laboratory of Agricultural Microbiology, Huazhong Agricultural
            University, No.1 Shizishan Str. Hongshan District, Wuhan, Hubei
            430070, China
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Newbler v. V2.3
            Sequencing Technology             :: 454
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009961205.2-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 03:13:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10
            CDSs (total)                      :: 9
            Genes (coding)                    :: 8
            CDSs (with protein)               :: 8
            Genes (RNA)                       :: 1
            tRNAs                             :: 1
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KC853436.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Escherichia coli ACN001"
                     /mol_type="genomic DNA"
                     /strain="ACN001"
                     /db_xref="taxon:1311757"
                     /plasmid="pACN001-C"
     gene            complement(join(5725..5784,1..96))
                     /locus_tag="G7C82_RS00005"
     CDS             complement(join(5725..5784,1..96))
                     /locus_tag="G7C82_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176703393.1"
                     /translation="MSVDFCDARQGGGAYGKTASALACSGALLFAHMFFPALSPDSVDN
                     RIIAFE"
                     /name="hypothetical protein"
     gene            300..458
                     /locus_tag="G7C82_RS00055"
     CDS             300..458
                     /locus_tag="G7C82_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072672540.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAAIGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(695..783)
                     /locus_tag="G7C82_RS00010"
     tRNA            complement(695..783)
                     /locus_tag="G7C82_RS00010"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(747..749),aa:Lys,seq:ctt)
                     /name="tRNA-Lys"
     gene            960..1850
                     /locus_tag="G7C82_RS00015"
                     /old_locus_tag="J444_pC2"
     CDS             960..1850
                     /locus_tag="G7C82_RS00015"
                     /old_locus_tag="J444_pC2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015376142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021512263.1"
                     /translation="MPDLEIMPLQSPDFYKKNKRAIYEGYKCNCTKDWKKEDRFVVYKA
                     DCTGIDEIINTEISDDNIDTVIKLAEKYTSDKIIISGGHTVVNLNDRFSVSNEVEKSAK
                     FCIDYIIKSTHELNIKPDFLMEINDFYMEKSNGEDIDGGNIYRKLATSPYIIPEVINNY
                     IIEKQNQHNIKINCFYVSEKNMADRFKRHIKRKEKEKPFFKENNSVFMNVDGSSFEVIK
                     NNKPTCAAGNAATFRSIRYKISSNKTFDNYTSHIGVFPLCSMANVINGYKAAASFYSNF
                     NLPCLLIFFGTSCFK"
                     /name="hypothetical protein"
     gene            1838..2995
                     /locus_tag="G7C82_RS00020"
                     /old_locus_tag="J444_pC3"
     CDS             1838..2995
                     /locus_tag="G7C82_RS00020"
                     /old_locus_tag="J444_pC3"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_073028479.1"
                     /translation="MLQMKGSKMKINSIQWPVTFFATSFGAGIFFLPQTVGPKVIGTHG
                     TITILILSSLASLLGQFTFYRFILITDKKEYVSSAILFIGKKAANAISIAFLLSMLLIA
                     IINITTMINIICFSLSLNGSERIFVSLIISATLGSSFIFFNSKIEHLASGIAFPSLLIV
                     LFLSIYFYSHPAHPHSTEIQNISTSNLLTLFPVVLFSFNFSPCIQRFAQNKKDTAGDLR
                     FFMFGILLILIFISFIVFSLSNIFSPSDIKNMREINSDALSYSANVTKNSFVAYISILT
                     MILITSGAYTGTLTGVVDTIVSLGFKNKTLIVFVVSVITFIAGEANFEIIKVIAALSTP
                     IIAITVFIIPSAFFLKTNCNSIFRKIMMTLNLLIGIGICFLAIIL"
                     /name="hypothetical protein"
     gene            complement(3389..3622)
                     /locus_tag="G7C82_RS00025"
     CDS             complement(3389..3622)
                     /locus_tag="G7C82_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_004026237.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3629..4147)
                     /locus_tag="G7C82_RS00030"
     CDS             complement(3629..4147)
                     /locus_tag="G7C82_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_072665564.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTTSG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4185..4883)
                     /locus_tag="G7C82_RS00060"
                     /pseudo=""
     CDS             complement(<4185..4883)
                     /locus_tag="G7C82_RS00060"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4825..5148)
                     /locus_tag="G7C82_RS00040"
     CDS             complement(4825..5148)
                     /locus_tag="G7C82_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001430281.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5212..5403
                     /locus_tag="G7C82_RS00045"
     CDS             5212..5403
                     /locus_tag="G7C82_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_100783622.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGETL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(5489..5574)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          2..409
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.377"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5619..5784
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.018"
                     /coverage="96.0"
                     /evalue="9.64e-56"
     Typing          complement(3286..4835)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.374"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgagcaaaa agcaaagcac cggaacaagc cagcgccgat gccgtttttc cataggctcc
       61 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      121 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      181 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      241 atagctcacg ctgttggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      301 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      361 ccaacccggt aagacacgcc ttaacgccac tggcagcagc cattggtaac tggacaaggt
      421 ggattgagat attaagagtt cttgaagtgg tggcctaact gaggctacac tagaaggaca
      481 gtatttggta tctgcgctcc accaagccag ttaccttcgg gaaaaagagt tggtagctct
      541 tgattccggc aaacgaacca ccgttggtag cggtggtttt tttgtttaca ggcagcagat
      601 tacgcgcaga aaaaaaggat ctcaagagga tcctttgatc ttttttcaaa acccctctca
      661 attcaacaaa tggtggggga aacgatcaac aaaatggcgg agagggtagg attcgaacct
      721 acgatacgct tgtcgcatat ggcctcaaga gagccctgac ttagaccact cgcccacctc
      781 tccacaaaaa acaatacata tttaacaaat aacatccaag acactataag agaaaaaata
      841 gagacgcatc acataaataa aggagacatc aatgatcaaa cactaacatg atctctatca
      901 ttgatatgta taagtcataa ttagcaaaat attttctttg tgacaaagga agcataacta
      961 tgccagacct agaaataatg cccttacaaa gtccagattt ttataaaaaa aacaaaagag
     1021 caatatacga gggctataaa tgtaattgta caaaagactg gaaaaaagaa gacagatttg
     1081 ttgtatacaa agcagattgc acaggaatcg atgagataat aaacacagaa atatcagatg
     1141 acaacataga tactgtaata aaactagccg aaaaatacac atcagacaag atcattatat
     1201 ctggtggaca tactgttgtg aatttgaatg accgtttttc agttagtaat gaagttgaaa
     1261 aatcggctaa attttgcatt gactacataa taaaatcaac acatgagcta aatataaaac
     1321 ctgatttcct catggaaata aatgatttct acatggaaaa atcaaatggc gaagatatcg
     1381 atggtggtaa tatttataga aaactagcca catcacccta tataatacca gaagtaataa
     1441 ataactatat tatagagaaa cagaatcagc ataatataaa aataaattgt ttctatgtta
     1501 gtgaaaaaaa catggcagat cgttttaaac gacacattaa aagaaaagaa aaagaaaagc
     1561 cattttttaa agaaaacaac tctgttttta tgaatgtcga tggttcatca tttgaagtga
     1621 taaaaaataa caaaccgact tgtgcagcag gtaacgcagc tacatttcgc tcgattcgat
     1681 ataaaatttc atcgaacaag acatttgaca attacacatc acacataggt gtattcccat
     1741 tgtgctcaat ggctaatgta attaatggct ataaagcagc agcatctttt tatagtaatt
     1801 ttaatcttcc atgtttactt attttctttg gaacatcatg cttcaaatga aaggttcaaa
     1861 aatgaagata aatagcatcc aatggccagt aacattcttt gcaacatcat ttggggcagg
     1921 gatttttttc ctaccccaga ctgtaggccc caaagtcata ggaactcacg gaacaataac
     1981 aatactgatt ttatcatctt tggcatcttt attagggcaa tttacatttt atagattcat
     2041 tttaattaca gacaaaaaag aatacgttag ttctgccata ttatttatcg gaaaaaaagc
     2101 cgcaaatgct atcagtattg cattcctatt atcaatgctg ttaatagcaa ttataaacat
     2161 aacaacaatg ataaatatta tttgctttag cctaagtcta aatggttcag agcgaatatt
     2221 tgtgtcactg attatttctg caacccttgg ttcttctttt atctttttta attcaaagat
     2281 agagcatctc gcatcaggaa tagcatttcc atctttgctt attgtactgt tcttatctat
     2341 atatttctat tcacatccag cacatccaca ttctacagaa atacaaaata ttagtacaag
     2401 taatttactt actctatttc cagttgtttt atttagtttt aatttttcac catgtataca
     2461 acgctttgct caaaacaaaa aagatacagc cggtgattta cgttttttca tgtttgggat
     2521 actattaatt ttaatattta tctcattcat cgtgttctca ttaagcaata tattctcacc
     2581 ttcagacata aaaaacatgc gcgagattaa cagcgatgca ttatcatata gcgccaacgt
     2641 aacaaaaaat agtttcgttg cttacatttc catattaacc atgattttaa ttaccagcgg
     2701 tgcatataca ggtacactaa caggcgttgt tgatacaata gtatcccttg gatttaaaaa
     2761 caaaacatta atcgtttttg tcgtatctgt tatcacattt atagcaggcg aagccaattt
     2821 cgaaattata aaagttattg ctgcattatc aaccccaatt atagcaataa cagtattcat
     2881 tatcccatca gcattctttt taaaaacaaa ttgtaacagt atttttagaa aaataatgat
     2941 gacgttaaac ctgctcatag gtattggaat atgcttccta gccatcatat tatgattatt
     3001 cctcacccaa atattctgac gagtttatct taatcgtcac aaccaaatgt ggaaaatggg
     3061 gggatcatcc aaaccattta acataacaac aatattgtac gcaccgctaa aacgcgttca
     3121 gcgcgatcac ggcagcagac aggtaaaaat ggcaacaaac cacccgaaaa aatgccgcga
     3181 tcgcgcctga taaattttaa ccgtatgaat acctatgcaa ccagatggta caggccacat
     3241 tccccccact taatccactg aagctgccat ttttcatggt ttcaccatcc cagcgaaggg
     3301 ccatccagcg tgcgttcctg tatttccggc tgacgctccc gttccaggga taacacatgt
     3361 tcgcgctcct gtatcaaccg ttcctctctt atctccagtt ctcgctgtat aactggctca
     3421 agcgttctgt ctgctcgctc aagtgctgca cctgctgact caactgcatg acccgctcgt
     3481 tcagcatcgc gttgtcccgt tgcgtaagcg aaaacatctt ctgcaattcc acgaaggcgc
     3541 tctcccattc gctcagccgc tgcatatagt cctgttgcag ctgctctaag gcgttcagca
     3601 aatgtatttc cagctctgtc actctgtgtc actccttcag atgtacccac tctttcccct
     3661 gaaagggaat cacctccgct gatttcccgt acggaaggac aaggaatttc ctgttcccgt
     3721 cctgcacaaa ctccacgccc catgtcttcg cgttcagttt ctgcaatgtc tcttcctgct
     3781 tcctgatttc ttccaggttc gtctgtatcc gtccgccaag ataccagagc gtcccgccac
     3841 tcgtggtaaa caggagaaag actatcccca gtaacatcat gcccgtattc cctgccagct
     3901 ttaacacgtc cttcctgtgc tgcatcatcg cctctttcac cccttcccgg tgtttttcca
     3961 gcgatttctc tgtcgaggct gtgaacaggg ctatagcgtc tctgattttc gtctcgtttg
     4021 aggtcacagc ctcgctgaca gactcgccga gcttcctgaa ctcgtcgttc agcattttct
     4081 ctgtagattc ggctctctct ttcagctttt tctcgaactc cgcgcccgtc tgtaaaagat
     4141 tgctcataaa atgctccttt cagcctgata ttcctcccgc cgttcgggtc tgcaatgctg
     4201 atactgcttc gcgtcaccct gaccacttca agccccgcct ctgtgagcgc ctgaatcaca
     4261 tcctgacggc cttttatctc tccggcatgg taaagtgcgt ctataccgcg tgtaacgctc
     4321 tcagcaagtg cctgtttcgt ttccggcagg ttatcaggaa gggtcagcgt ccggcggttc
     4381 tccggggcgt tggggtcatg cagtccgtaa tggtgattca ccagcgtctg ccaggcatca
     4441 attctcggcc tgtctgcgcg gtcgtagtac ggctggaggc gttttccggt ctgtagctcc
     4501 atgttcggaa tgacaaaatt cagctcaagc cgtcccttgt cctggtgctc cacccacagg
     4561 atgctgtact gatttttttc gagaccgggc atcagtacac gctcaaagct cgccatcact
     4621 ttttcacgtc ctcccggcgg cagttccttc tccgcgaacg acagaacacc ggacgtgtat
     4681 ttcttcgcaa atggcgtggc atcgatgagt tcccggactt cttccggatt accctgaagc
     4741 accgttgcgc cttcgcgatt acgctccctc cccagcaggt aatcaaccgg accactgcca
     4801 ccaccttttc ccctggcatg aaatttaact atcatcccgc gccccctgtt ccctgacggc
     4861 cagacgcagc cggcgcagct catccccgat ggccatcagt gcggccacca cctgaacccg
     4921 gtcaccggaa gaccactgcc cgctgttcac cttacgggct gtctgattca ggttatttcc
     4981 gatggcggcc agctgacgca gtaacggcgg tgccagtgtc ggcagcctcc cggaacgggc
     5041 aaccggctcc cccaggcaga cccgccgcat ccataccgcc agttgtttac cctcacagcg
     5101 ttcaagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctcgcgttt
     5161 catcggtatc attaccccat gaacagaaat cccccttaca cggaggcatc agtgactaaa
     5221 caggaaaaaa ccgcccttaa catggcgcgc ttcatcagag gccagacatt aacgctgctg
     5281 gagaagctca acgaactgga cgcggatgaa caggcagata tctgtgaatc gcttcacgac
     5341 cacgccgatg agctttaccg cagctgcctt gcacgcttcg gggatgacgg tgaaaccctc
     5401 tgacacaagc agcttccgga gacgctcaca gcttgcctgt gagtggatgc cgggagcaga
     5461 caagccctcc agggcgcgtc agcaggtttt agcgggtgtc ggggcgcagc catgacccag
     5521 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagtg cagattgtat
     5581 gaaaagtgcc tcatgccggg tgtgaaattc cgcacagatg cgtcacagat gcgtaaggag
     5641 aaaatgcgcg tccaggcgct tttccgcttc ctcgctcact gactcgctac gctcggtcgt
     5701 tcgactgcgg cgagcggtgt cagctcactc aaaggcgata atacgattat ccacagaatc
     5761 aggggataac gccggaaaga acat
//
