LOCUS       NC_022741               1549 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Escherichia coli plasmid pHUSEC2011-3, complete sequence.
ACCESSION   NC_022741
VERSION     NC_022741.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3865278
            Assembly: GCF_000967155.2
KEYWORDS    RefSeq; complete replicon.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Kuenne,C., Billion,A., Mshana,S.E., Schmiedel,J., Witte,W.,
            Domann,E., Hossain,H., Hain,T., Imirzalioglu,C. and Chakraborty,T.
  TITLE     Impact and evolution of plasmids of german Escherichia coli
            HUSEC/EHEC epidemic isolate HUSEC2011 in relation to putative
            ancestor HUSEC41
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Kuenne,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2011) Medical Microbiology, Justus-Liebig
            University, Frankfurterstr. 107, Hessen D-35392, Germany
REFERENCE   3  (bases 1 to 1549)
  AUTHORS   Kuenne,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2011) Justus-Liebig University, Medical
            Microbiology, Frankfurterstr. 107, Hessen, D-35392, GERMANY
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000967155.2-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 04:04:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,385
            CDSs (total)                      :: 5,263
            Genes (coding)                    :: 4,980
            CDSs (with protein)               :: 4,980
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 283
            CDSs (without protein)            :: 283
            Pseudo Genes (ambiguous residues) :: 0 of 283
            Pseudo Genes (frameshifted)       :: 109 of 283
            Pseudo Genes (incomplete)         :: 187 of 283
            Pseudo Genes (internal stop)      :: 60 of 283
            Pseudo Genes (multiple problems)  :: 64 of 283
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            HE610902.1.
            On Dec 11, 2015 this sequence version replaced NZ_HE610902.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="HUSEC2011"
                     /serovar="O104:H4"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pHUSEC2011-3"
                     /geo_loc_name="Germany:Frankfurt am Main"
     gene            complement(286..411)
                     /locus_tag="HUS2011_RS28830"
     CDS             complement(286..411)
                     /locus_tag="HUS2011_RS28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001431667.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
                     /name="hypothetical protein"
     gene            914..1390
                     /locus_tag="HUS2011_RS28450"
                     /old_locus_tag="_p106002"
                     /old_locus_tag="HUS2011_pIII0001"
     CDS             914..1390
                     /locus_tag="HUS2011_RS28450"
                     /old_locus_tag="_p106002"
                     /old_locus_tag="HUS2011_pIII0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          433..531
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          complement(727..835)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.091"
                     /coverage="100.0"
                     /evalue="9.09e-49"
     Typing          complement(73..333)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.985"
                     /coverage="100.0"
                     /evalue="1.85e-99"
ORIGIN
        1 ctaccggaaa ccgtctgggg agaaccccag acccccatcg ctgcaacacc gacggtctac
       61 gaccacctac caaccgcatg gctgcctgtt ccgtgcaaat gggctttgca gggggctggc
      121 gcggttcctg gcgacctgaa agcccctggt taggggtatc ggcgcgcttg acctcctgag
      181 agcctctgta agcgtttttc gcgccttcca tgccctggcg ggcatctaaa acccgtttcg
      241 tgcgtctgag agcttttgag cgcgtttgag gggctatctg gcgactcatc cccaaaagaa
      301 caacaccggg atcaccgaca acatgcgaga ggtgatcccg ctcgccgcaa gtcgatgacc
      361 gagcgtagcg agcgaatcga cgaggaagcg gaagagaact ggaagccaca ttgagcactt
      421 acgcactgat gcggggtgtc ggggtgaagc cctgaccaag tggtaatcgt atcggcgtgc
      481 atgcgcggtt atacgattac acatcctgtc ccgatttctg aggcgtttta acggtgaacg
      541 gacggcaaaa aaacgatgga gctgttgaac gtgaaaacca cgaaatctgg caaaaatcac
      601 ggctggcgag gcttgcatag gagtaagcca gtatacactc cgctatcgct acgtgactgg
      661 gtcagggctt cgccccgaca cccccaaagg gcgttgcgtt gcacgcaaca cccttgccta
      721 gaatagatct tttacgccga tttgtaagtg gttgtttttt tagtgtgttt tttatttacc
      781 tgtcgcatcc aggatgacac aacgttgtcg catcctggat gcgacaacac gcaacaaggt
      841 gttgcataga tgtgtcatga tggcttagat ttgaaccaac gttagtgcat gggattttca
      901 gagggaaaaa atcatgttta ttgattcaga aaaacgactg aaacaacttt cagatgaggc
      961 aaagaaaaac accgaggatc tcgaagaagc aaagaaaaat tcaaggttta cacaggtatc
     1021 cccaaaaggt tgggaacgtg ttcgagagct gctgaaggat agccaaggca tatcagcact
     1081 gaagctgtac tcatttttag cggagcatat cgatcctacg tgtggcgctg tcgttgcgga
     1141 tcagcaattt ctagctgaaa aacttggagt tagcagaagc acaattattc ggtggctcaa
     1201 ttacttagaa tcaaaaaatg cattagttag aatccccgtt gctggtaagg tttgtgcgta
     1261 tgccctcgat ccacatgaag tctggaaggg atacaacact acgaaaaacc atgcagcgtt
     1321 tgtcactaaa acactggtca acaaagacgg tgatattcag cgccgaatca tggccatgtt
     1381 ttcaaattga gctagcggca ggcggacaat caggggctac gtgttaacgt tctgaccatg
     1441 attgtctatc ctgcattgct cttttgccgc ctcaaaatcc tttgcgtgtt tttgctcccc
     1501 gttctccaaa aaaaaccgag ccgccacggt tccggccgcg ccttgagcg
//
