LOCUS       NC_022649               5167 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Escherichia coli JJ1886 plasmid pJJ1886_2, complete sequence.
ACCESSION   NC_022649
VERSION     NC_022649.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02603887
            Assembly: GCF_000493755.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli JJ1886
  ORGANISM  Escherichia coli JJ1886
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Andersen,P.S., Stegger,M., Aziz,M., Contente-Cuomo,T., Gibbons,H.S.,
            Keim,P., Sokurenko,E.V., Johnson,J.R. and Price,L.B.
  TITLE     Complete Genome Sequence of the Epidemic and Highly Virulent
            CTX-M-15-Producing H30-Rx Subclone of Escherichia coli ST131
  JOURNAL   Genome Announc 1 (6), e00988-13 (2013)
   PUBMED   24309736
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Andersen,P.S., Price,L.B., Sokurenko,E.V., Stegger,M., Azziz,M.,
            Contente-Cuomo,T., Gibbons,H.S., Keim,P. and Johnson,J.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2013) Microbiology and Infection Control, Statens
            Serum Institut, 5 Artillerivej, Copenhagen S DK-2300, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: MIRA v. 3.9.9; ABySS v. 1.3.4; PBJelly v.
            12.9.14; CLC-bio v. 6.0.4; PAGIT v. 1; Celera v. 7.0
            Genome Coverage                   :: 50x
            Sequencing Technology             :: 454; Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000493755.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 02:00:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,283
            CDSs (total)                      :: 5,168
            Genes (coding)                    :: 4,961
            CDSs (with protein)               :: 4,961
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 76 of 207
            Pseudo Genes (incomplete)         :: 144 of 207
            Pseudo Genes (internal stop)      :: 43 of 207
            Pseudo Genes (multiple problems)  :: 48 of 207
            Pseudo Genes (short protein)      :: 1 of 207
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP006786.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Escherichia coli JJ1886"
                     /mol_type="genomic DNA"
                     /strain="JJ1886"
                     /db_xref="taxon:1355100"
                     /plasmid="pJJ1886_2"
     gene            complement(17..931)
                     /locus_tag="P423_RS25865"
                     /old_locus_tag="P423_25135"
     CDS             complement(17..931)
                     /locus_tag="P423_RS25865"
                     /old_locus_tag="P423_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            2668..2877
                     /locus_tag="P423_RS25870"
                     /old_locus_tag="P423_25145"
     CDS             2668..2877
                     /locus_tag="P423_RS25870"
                     /old_locus_tag="P423_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(2943..3476)
                     /locus_tag="P423_RS25875"
                     /old_locus_tag="P423_25150"
     CDS             complement(2943..3476)
                     /locus_tag="P423_RS25875"
                     /old_locus_tag="P423_25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3479..4405)
                     /locus_tag="P423_RS25880"
                     /old_locus_tag="P423_25155"
     CDS             complement(3479..4405)
                     /locus_tag="P423_RS25880"
                     /old_locus_tag="P423_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            4582..4980
                     /locus_tag="P423_RS25885"
                     /old_locus_tag="P423_25160"
     CDS             4582..4980
                     /locus_tag="P423_RS25885"
                     /old_locus_tag="P423_25160"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     Typing          complement(157..931)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(3483..4405)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tataggctta tttggtctac tctacgagac ctcgtttttt cagttggtaa taccaggcgc
       61 gactgatccc taatgcctcc cacggtttca gcgtgcgtgc agatgtagct actgtgctgc
      121 gcttagactt agaaccgccc ttgcgaccgc gtgcagcctg aatttctggc gtgtgcgtat
      181 cagccacata ctgtgcgaaa gtttccggcg tgaagttcct gtgcgtgtat ttcgcaatgc
      241 tcttgccaat agcccgacat tcagccagtg aaagcggaac gggaagcgat gcattgtaca
      301 tttcgacacg ctggatcacc gcttcaagcc actgtgagaa cgctggccag ccctgacgaa
      361 tcgccctgta agcccatttg cgcgtcattt cgaacaggtg gcagtttcgc cccatcccgt
      421 aatgtttatc gatgctacga cgctctgagg cgctcaaatc gagataatca gccagttcat
      481 cgagggtata ggcttcctcg cgccactcca tcaccagcca ttccaggtgg aacggatttt
      541 tgcaaatcag gccgctgtag ttcacatccg cacaaagttt ctcacacagc gaacgttcaa
      601 ccgctgcggc atatttcagc gccttaaccg aggcatcagg cgcggttctc acggcgatgt
      661 tgagtgcgta gagcaggtga gcgtgtccgt tcacaggatt tttaacggtg atgttcggcg
      721 cgggagcgtt ccggtcgctc cagtcaatcg cagccccctc gcggtcaacg tcaaaaacca
      781 gccagaactg cgcatgaggc tggttctgct gtatgtaccg cgcaagaagc gcacgccctt
      841 tgccggagat gcgaagacca aaagcgagat catccgtgtg atagggacga tggggtaaat
      901 tttcgtcgaa gtattgaagc gcggcgctca tgtgttacga ttctctccgg gttatgcgac
      961 ctacacgccc tctgtatttc atgttgaatc ttgccggatc tggacatgat ttcaggggcg
     1021 tttgtttttc agtcgcttac actacaccca acggcaaatc ctgtcgaacc ttcccgaaaa
     1081 cccgatccat ttaccataac tcccgttgta cgcccccctt aaaacgccct cagtcgcttt
     1141 ctggcgcaaa aaacgcaaaa agacaagaaa acaccaggcc aaaccagaaa aacgcgtcag
     1201 aatcgttttc agggcgttgc ataactatgc acgaaagcga tgtgcgatcg gtttcgtgcg
     1261 ttctgaacac cgaacggctc aaaacgacaa gttttctccc ctggtgcgtg gcgctctgac
     1321 ggggtttcct gtttccaaac ttcacatctc gccaggacaa aaaaaaaccg acctggggtc
     1381 ggtggcaaga atcagagtct atgggaaata ttttagttat cggatgctca atattcacaa
     1441 acgcaaaaaa tgcctcagtg atgccctaaa acaacctttc cagaaggagt ccgcgcttta
     1501 cgatgcacgg aagagataaa aaaccccaca aaagtgggga accaagagaa acaacaatta
     1561 caggttgttg gttgtgttcg aacaacgatg aagtagctac gctaaataat aattaccgta
     1621 caggccctac atattcaaca ctaaatttaa gatcctgacg aaggatttat aaatgcacga
     1681 aaacacatag acagacaaat tcactaaatt catacacaaa aacaagtgta gttttctaag
     1741 taaattagat tcgacaacca attagtttat ccacatcgca ttccactctt gctacaccaa
     1801 tcacatttct tgatatgtaa tagcagaaaa aataaaacca gacaccaata gaccaaaaaa
     1861 caaacattca acaagaaaca ttttgaacaa tatcacaaaa ctgtcacaac catcatcaga
     1921 ctattgaaaa aaactggttg ctgttttata aaagcgactg taacaacctc cactctatag
     1981 ttccattagt ttaaagtgtc acacaaactg agcatccatt tgttttcctt acttttgccc
     2041 ctgtattcca aggggttttt ttataactta aagattagca caccacacaa aaacaataaa
     2101 aaaaaacgac aagttataca ataaaaccag tactatccat acagctaatt acatataaat
     2161 actattaaac catctggaca ttaaaaataa actcttataa cataaaatta atgcggttcc
     2221 ggtaccatat gtcagggata atgtgcaaat ggaggtatcg gaactgcatt tcagttcatt
     2281 attaattaat gacaaaaaaa acctctgcga gcgcagaggg ggtaaggaat gttcccggtc
     2341 ataatatgtc gatagtgttt atatacatac agaacaagaa caacaaccag ccaactaata
     2401 aatattttat tttttagagc tgaaacctta aagctcactt ccattccata cattgttata
     2461 aaccttatgt tgaaaactga aagtaagtac ggcagactta ttaagttgcg tgcagtcttt
     2521 tcacgttata caaacaagaa cacctgcgaa gttatttctc cttacatatc cccctatgcc
     2581 aacgggggat tttttctggt tcactaacgc ataaataata gtgacaccct atcattcaca
     2641 aaccgtgata atataaggat tatcatcatg gcaaaattag gtgaaaacac cccacatatt
     2701 atcgatgcta ctgtagattt tatggcatca agccaggcat ttcgtgagta tctgaaaaaa
     2761 cttccccctg ataacacaat accgccagct cttactgatg agcacgtacc gttatatata
     2821 cgaaggctgg aatactatcg tctgctttat cgaccaaagc agacagaatc atattgaaaa
     2881 aaacggtgaa cggttcgcga aattctttcg cgggtcgtga ggcgttggac aagcgaagcg
     2941 cgtcagtttt tccggctctt ccccgtgaca ggtttctcca ccagtttatc ttccagtgtc
     3001 acaactttct gatacaccgc cgctgtggcg ttgtagttcc tgtcgagtat tgttttcatt
     3061 ccccatccga gcacacagaa cataaccaga aaagccactg acaccccacc cagccatttc
     3121 cagctatacc ctttaagggt gttgtattcc tgcacgatat tgctgtgaat tgtcgcaata
     3181 tccctgttca gggcatccgc gtttgccttc atgtttgcga ctgactcttc cagccgctga
     3241 aaatcctggc tgatgcgctc acctatcagt cctgccacgt ccagcttctg cacctcattt
     3301 ctgacttcat ccctgacctg ttttgccaca ccgtcagaaa tatcccgata tccgcgaaga
     3361 gtttcccttg ccacagccag agaatcagca acagctttct gttgctccat tgcggtatgt
     3421 tgcagatgct ccaccgataa caggagcgtg agtaattttt cttctgtgct ggccatgttc
     3481 agaatcccat agaaaatcca cgtgacggtg atttaacccg ttcaggtgca gttcggttct
     3541 gtctgctcac tctgtctctc atgtcaaagc catgctcccg cgcaacatca tgttgcagcc
     3601 ttctgaccag ttcatgttgc ttaaccggag tgtccagcca ttccctgatg tcatgcccga
     3661 ccgttcgcgc ctgttcaaga tggcgggaat agtccggttc agaccgtttt tgcatgatgt
     3721 catgacgctg ggcatcatca agccgtccgg cagcttcaaa acccagatgc acttcgggac
     3781 gtcgttcaat gccctgttct ttcagcgtcc ggcagtcaat gaagcccctt tcaaagccat
     3841 gctttctcat atgttcgttg tgcttcacct cccagcgttt tcgcaattcc cgcaactgct
     3901 ctttttgctg agtgagagtc aggcttccgg aatccttttt cgcacctccc cgctcgggat
     3961 acctggcgtt atagcgccgg aaatactgtt ccggtgtacg ttcaatgcca tcattcacac
     4021 gctgactcat catgatatgc gcatgaggct gttcgccgcc gtcaatgctc gctttcggat
     4081 tgtgaatggc aaaactccag gcgtgccgtt cgccagcttc ctgacgtaca aaatcctgca
     4141 ccagttcaag gcgctgttcc ggtgtcagtt cacggggtaa tgcaatttca agttccctat
     4201 aggtcgaacc gttcgcacgt tcaaactggt cagccgcctg ccagaaatgg gaaggattat
     4261 ccctggccca ttccggcatg tttccgtgtg cggaatattc caaatcctga cggtcgcggt
     4321 atttctcttt gcgctcgatg taatcggcgt ggtttgcagc ctgccccttg ccaccaaatt
     4381 taacgctgag gtgataggtt gccacggttt cagccctctg caggagcgca ctgtttctcg
     4441 acgaaggaga gaaacatata agtgcgcttc cttctaattt tacgaattag cacggaagat
     4501 accccctgca cccgccgacc gcaagcgata tactgcacga ccacaacaca ccgcaaaaaa
     4561 acaccaggga gcatcacgaa catgcgcatc agcaacatcg aatggctgaa gaaacggatc
     4621 ggattcatca ggaagctcgg agaacagacc gcacgacaga gacagattat tgacctgatc
     4681 gacaacgaag ccggactcac agaacaggaa cgcaaacttc tccacgtact ggcaacagcg
     4741 gaaaaaaacg acttacaggc acaggagagc gaacgaaaac aggcggtcca aaaacgaatc
     4801 gagggaaaaa aacaacgacg ggaacgcaat caccggctct ttctcgcggc tgggctactg
     4861 atcgaagcag gactggtaga cacaaaaaca ggcgagctat gctataaaaa agacaggata
     4921 ttacaagcac ttaaggaact taagtacgat ctggagacat ccccaaaccc agacgcgtaa
     4981 gtgcctccat ccgtgttccg attccctggt gcttcctcgc tcattcgctc gctacgctcg
     5041 gtcatgagac tgcggcgagc actaccacca caatcacgcg gaaagccgcg ccattgcagg
     5101 gctgaacggg gaaaaacggc aaaaaacctg ctgctcaaaa agacgccaga aaggcgctgt
     5161 tatctga
//
