LOCUS       NC_022376               8275 bp    DNA     circular CON 04-MAY-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Derby str. T12 plasmid
            pST12, complete sequence.
ACCESSION   NC_022376
VERSION     NC_022376.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14229286
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Derby str. T12
  ORGANISM  Salmonella enterica subsp. enterica serovar Derby str. T12
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1
  AUTHORS   Vignoli,R., Cordeiro,N.F., Garcia,V., Mota,M.I., Betancor,L.,
            Power,P., Chabalgoity,J.A., Schelotto,F., Gutkind,G. and Ayala,J.A.
  TITLE     New TEM-derived extended-spectrum beta-lactamase and its genomic
            context in plasmids from Salmonella enterica serovar derby isolates
            from Uruguay
  JOURNAL   Antimicrob Agents Chemother 50 (2), 781-784 (2006)
   PUBMED   16436745
REFERENCE   2  (bases 1 to 8275)
  AUTHORS   Cordeiro,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2013) Bacteriologia y Virologia, Instituto de
            Higiene, Facultad de Medicina, Alfredo Navarro 3051, 3er piso,
            Montevideo, 11600, URUGUAY
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009955995.2-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 06:25:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9
            CDSs (total)                      :: 9
            Genes (coding)                    :: 8
            CDSs (with protein)               :: 8
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 1 of 1
            Pseudo Genes (incomplete)         :: 0 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            HG428760.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Salmonella enterica subsp. enterica serovar
                     Derby str. T12"
                     /mol_type="genomic DNA"
                     /strain="T12"
                     /serovar="Derby"
                     /sub_species="enterica"
                     /db_xref="taxon:1401650"
                     /plasmid="pST12"
     gene            complement(205..318)
                     /locus_tag="HXG55_RS00045"
     CDS             complement(205..318)
                     /locus_tag="HXG55_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001459398.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAAIGN"
                     /name="hypothetical protein"
     gene            522..677
                     /locus_tag="HXG55_RS00005"
     CDS             522..677
                     /locus_tag="HXG55_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001490772.1"
                     /translation="MSVDFCDARQGGGAYGKTPAALASSGALLFAHMFFPALSPDSVDN
                     RITAFE"
                     /name="hypothetical protein"
     gene            complement(897..3902)
                     /locus_tag="HXG55_RS00010"
     CDS             complement(897..3902)
                     /locus_tag="HXG55_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143760.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            4066..4623
                     /locus_tag="HXG55_RS00015"
     CDS             4066..4623
                     /locus_tag="HXG55_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            4806..5666
                     /locus_tag="HXG55_RS00020"
     CDS             4806..5666
                     /locus_tag="HXG55_RS00020"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase TEM-144"
                     /protein_id="WP_021598056.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDCW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGKRGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="extended-spectrum class A beta-lactamase TEM-144"
     gene            complement(5805..5954)
                     /locus_tag="HXG55_RS00025"
     CDS             complement(5805..5954)
                     /locus_tag="HXG55_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001677812.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EEGV"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6239..6562
                     /gene="mobA"
                     /locus_tag="HXG55_RS00030"
     CDS             6239..6562
                     /gene="mobA"
                     /locus_tag="HXG55_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(6797..7177)
                     /gene="lsoB"
                     /locus_tag="HXG55_RS00035"
     CDS             complement(6797..7177)
                     /gene="lsoB"
                     /locus_tag="HXG55_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin LsoB"
                     /protein_id="WP_000710826.1"
                     /translation="MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIESDLKKKK
                     SKGDVFFDLIIPNGGKKDRYVYTSFNGEKFSSYTLNKVTKTDEYNDLSELSASFFKKNF
                     DKINVNLLSKATSFALKKGIPI"
                     /name="lsoB"
     gene            complement(7174..8215)
                     /gene="lsoA"
                     /locus_tag="HXG55_RS00040"
                     /pseudo=""
     CDS             complement(7174..8215)
                     /gene="lsoA"
                     /locus_tag="HXG55_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA
                     endoribonuclease LsoA"
                     /name="lsoA"
     ARG             4807..5663
                     /name="blaTEM-144"
                     /gene="blaTEM-144"
                     /gene_name="extended-spectrum class A beta-lactamase
                     TEM-144"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          5990..6230
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.12e-123"
     Typing          complement(247..776)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.972"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          6240..6559
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.19e-62"
ORIGIN
        1 gctgttcgtt gacttgatag accgattgat tcatcatctc ataaataaag aaaaaccacc
       61 gctaccaacg gtggttttct caaggttcgc tgagctacca actctttgaa ccaaggtaac
      121 tggcttggag gagcgcagtc accaaaatct gttctttcag tttagcctta acaggtgcat
      181 aacttcaaga caaactcctc taaatcagtt accaatggct gctgccagtg gcgataagtc
      241 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
      301 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
      361 ccaacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
      421 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      481 ctggtatctt tatagtcctg tcgggtttcg ccacctctgg cttgagcgtc gatttttgtg
      541 atgctcgtca ggggggcgga gcctatggaa aaacgcctgc ggcgttggct tcttccggtg
      601 ctttgctttt tgctcacatg ttctttccgg ctttatcccc tgattctgtg gataaccgta
      661 ttaccgcttt tgagtgagct gacaccgctc gccgcagtcg aacgaccgag cgtagcgagt
      721 cagtgagcga ggaagcggaa gagcgcctta tgtgacattt tctccttacg ctctgttgtg
      781 ccgttcggca tcctgtcctg agagttatct ctctgtgcta ttttctactt caaagcgtgt
      841 ctggatgctg ttctggagtt cttggggtct gacgctcagt ggaacgaaaa ctcacgttaa
      901 gcaacgtttt ctgcctctga cgcctctttt aatggtctca gatgtccttt ggtcaccagt
      961 tctgccagcg tgaaggaata atggccgagc atattgatat gtccgtggca aagcggggag
     1021 aggcgtgcga tatcttcatc attcagtgtt tcaccctgcg cccggagatg atccagggct
     1081 gcctgcatat aaatagtgtt ccataacacg acggcgttag tgaccagccc cagtgtgccc
     1141 agttgatctt cctgaccgtc ggtatatcgt tttcttatct cacctttttg accgtgacag
     1201 atggctctgg caacggcatg gcgactttct ccccgattaa gctgggtcag aatgcgccgg
     1261 cggtaatctt catcatcaat ataattaagc agatacagcg ttttgttgat gcgccccact
     1321 tcaatgattg cctgagtcag tccggaagga cgttcacttt tcagcaatga acggaccagc
     1381 actgaaacct gtactttgcc cagcttcagg gagccagcgg tccggatcat ttcgtcccac
     1441 tgaaggacta tttttcgggg atctgattgc cctctggcaa tatcattcag cacgccatag
     1501 tcggcatcat ggtccattcg ccagaaaacc gaagcaccgg catcagccag gcgtggagaa
     1561 aactggtatc ccagcagcca gaaaaggcca aagacaagtt cgctggcacc tgctgtatcg
     1621 gtcataattt cggttggatt cagcccggtc tcctgttcca gaagaccttc cagcacaaag
     1681 atagagtccc tcagcgtccc cggtataacg atgccatgaa agccggaata ctgatcggac
     1741 acaaagttgt accaggtgat ccctctgtta ttaccaaagt atttgcggtt cggtccggca
     1801 ttgattgttc tgactggcgt aacaaagcgc attccatctg cagatgccac ttctcctcca
     1861 ccccatatct gtgccagtgg cagcgttgcc tgaaaatcaa ccagtctggc attagcgctg
     1921 gtgatagttt cagcccgcag atagttcgct tttgtccagt tcagccggtg tcgggtcagt
     1981 gcaggaacat ttgatctgat cagtggttcc agaccgatat tgcaggcttc agccatcagc
     2041 acggcgctga tgctgacggg cagatcatca actctggcac tggcttcact agcatggaaa
     2101 aactcatcag caaatccggt atgggcgtta atttcgagca gcaactccgt taaatccacc
     2161 ggagggagta gatcactgat cattttgctc agtcgtttca gactgtccgg ctcatcaaga
     2221 ctggcgaggg gagaaattgt caaccggggc ttcgggccag aaacatcgag ttcgacagcc
     2281 tcattttcgc aaagacgtgc agcaacctgt ctgtaacgac tatcaagctg atgacccaga
     2341 gattttattg cttcctgcgg gtctgtcggg tgccccaaag aacgataaac cttaatccgg
     2401 tttgcctgcc agtcagcacc ctgtagtaat cttgcacgag gatctcccca ccggttactg
     2461 ccggtaacgt agacatccct ccgcctcaga ctatcctgca gtttactgag aaagcagagc
     2521 gtgtatcccc tgcgggtgat atgtttttcc ttgttaatca ccagccgttt ccatgaccga
     2581 ctgataattt ccgttggtgc gtcgtcaaaa aactgccgcc gtgagctgaa ctcccggctg
     2641 aggtagtcac aggcattcag agtggtaacc ccggcaggtg cggatgaaaa tttaacggta
     2701 ttcagcagat ggggcaggaa acgacgaacg cgcccgtact gctccaccat ttcttcatga
     2761 aaattatcgt ctgagggccg ggcaatttca cggacaagcg tgatgatttc agccagcttt
     2821 tgccttggga tgtagctgaa cacctcagca cgaatcgatt cgtccggtgt ttcttctttc
     2881 agcaggtacg aacatgcgct ggcgagcgcc aatgcagatt tatccagatc cttcagcgag
     2941 cggagccgtt ttttctgccc aatctttctg gcgtcacgga tgataacggc cagcatggcg
     3001 tccagaacgt ccaatgcatc atccagcgcc agcgtttccc atgcaaggac aaaggcaacc
     3061 agaaccgcca tccttttctg cggtgacatc ctggcaatat tgaacaccga agtcatacca
     3121 gcataacgtg cgagattttt caggcgcaca gccgggagtg tactcaggtt ttcagcatgc
     3181 aggccaaaat cgttcagagt tttccagcgt tcaattgctt cattaaacgc cggaccactg
     3241 atggtcacag ggcccttttt cagtgattcc agtaaagaca ggcggctgca atcagttggc
     3301 cccagcagca tctccagctg tgaacgctgt tcggctgacg gtatcagtgc cagtttgttc
     3361 cacaggcgca acgtcgcctt ttcccttacc tctgaaatca accgggtcag cgtagtggct
     3421 ccggggagaa taatacgatg ttgcataagc caccctgtcg ccagatcgaa aagcaggcca
     3481 ggacgttcgt tgcttatcca gctccgggta tataaaagac gggtaaggcg aaatgtccag
     3541 ggccaggcaa attcacgata ctgatagtgc tgacgtatca gcgctgcatg ctcacggcgg
     3601 gtattttccc tctgaccgta ttctgcaaga acggtgatat cacgaatccc gagctgtctg
     3661 gcggtaaaat gccggacgcc ggaaggaata tgattcatat cggtgaggaa ggtgcccaga
     3721 aaacggacac atccaatttg cagggcaatg cccagacggt tgtgatcacc tctgcttttt
     3781 ccgataaatt ccttgtctgc ttcatcaagg tgaaaatatc gtgccagctg aagctcatcc
     3841 ggttcaccgg tgaatctgcc atagctttca gtctgctcag tggtcagaaa gtcaacgggc
     3901 atatcggcct ccctgcctga cgggcattta gtaacatttt tccaaccgta cgaaatgtta
     3961 taaattatcg gacatcgtaa aactgttaca ttaatatgtc tattaaatcg taaatttgta
     4021 ataatagaca tgagttgtcc gatattcgat ttaaggtaca tttttatgcg actttttggt
     4081 tacgctcggg tctcaaccag tcagcagtct cttgatcttc aggtcagagc actcaaagac
     4141 gcaggtgtga aagcaaaccg tatatttacc gataaggcat ccggcagttc aacagaccgg
     4201 gaagggctgg atttgctgag gatgaaggtg gaggaaggtg atgtcattct ggttaagaag
     4261 ctcgaccgtc ttggccgcga cactgccgat atgatccaac tgataaagga atttgacgct
     4321 cagggcgtgg cagtccggtt cattgatgac gggatcagta ccgacggtga tatggggcaa
     4381 atggtggtca ccatcctgtc ggctgtggca caggctgaac gccggaggat cctagaacgc
     4441 acgaatgagg gccgacagga agcaaagctg aaaggaatca aatttggccg caggcgtacc
     4501 gtggacagga acgtcgtgct gacgcttcat cagaagggca ctggtgcaac ggaaattgct
     4561 catcagctca gtattgcccg ctccacggtt tataaaattc ttgaagacga aagggcctcg
     4621 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     4681 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4741 atatgtatcc gctcatgaga caataaccct ggtaaatgct tcaataatat tgaaaaagga
     4801 agagtatgag tattcaacat tttcgtgtcg cccttattcc cttttttgcg gcattttgcc
     4861 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4921 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4981 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggtgcggtat
     5041 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5101 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5161 aattatgcag tgctgccata accatgagtg ataacactgc tgccaactta cttctgacaa
     5221 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5281 gccttgattg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5341 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5401 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5461 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtaagcgtg
     5521 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5581 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5641 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5701 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5761 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccttcttct
     5821 gcgagttcgt gcagcttctc acacatggca gcctgttcgt cagcatcgag tgcgtccagc
     5881 ttttcgagca gcgtcaggct ctggcttttt atgaatcctg ccatgttgag tacagcttgc
     5941 tgctgcttgt tcatcttttc gttttctccg ttctgtctgt catctgcgtt gtgtgattat
     6001 atcgcgtacc acttttcgac tgttttaccg ccggtattct gccgtctgac gctttgacgg
     6061 gtatttctgc ctgacaatac tgtcactgcc aaaaaactgc cgtgcctttg tcggtaattc
     6121 gagcttgctg acaggacagg atgtgcaatt gttataccgc gcatacatgc acgctattac
     6181 aattaccctg gtcagggctt cgccccgaca ccccatgtca gatacggagc catgttttat
     6241 gacaaaacga agtggaagta atacgcgcag gcgggctatc agtcgccctg ttcgtctgac
     6301 ggcagaagaa gaccaggaaa tcagaaaaag ggctgctgaa tgcggcaaga ccgtttctgg
     6361 ttttttacgg gcggcagctc tcggtaagaa agttaactca ctgactgatg accgggtgct
     6421 gaaagaagtt atgcgactgg gggcgttgca gaaaaaactc tttatcgacg gcaagcgtgt
     6481 cggggacaga gagtatgcgg aggtgctgat cgctattacg gagtatcacc gtgccctgtt
     6541 atccaggctt atggcagatt aacttcccgg agagaaactg tcgaaaacag acggtatgaa
     6601 cgccgtaagc ccccaaaccg atcgccattc actttcatgc atagctatgc agtgagctga
     6661 aagcgatcct gacgcatttt tccggtttac cccggggaaa acatctcttt ttgcggtgtc
     6721 tgcgtcagaa tcgcgttcag cgcgttttgg cggtgcgcgt aatgagacgt tatggtaaat
     6781 gtcttctggc ttgatattat attggaatgc cttttttcaa agcaaatgat gtggctttgg
     6841 atagaaggtt tacgttgatc ttatcaaagt tttttttaaa gaacgaagcc gagagctcag
     6901 ataaatcatt atattcatca gttttcgtaa ctttgtttaa tgtgtaactt gaaaacttct
     6961 cgccattaaa tgacgtatag acgtaacgat ctttttttcc accgttagga attattaaat
     7021 caaaaaaaac atcacccttg cttttctttt tcttcaagtc ggattcgatt tttgagaaaa
     7081 attcgctcgg gctataaata tcagtagcat agacaataaa taaagtttta tctttatttt
     7141 ttattgcttc tatttgatat tttttatctt ttttcataat ttcaacctag ctacgccacc
     7201 atctattaat tggcaaacgg tatcgatgat tgcgattgat tctaatttgt taattgtctt
     7261 cgtgtcagct attcctggtt tcatatgaaa caaaccatgc ctgttctcat gccagtaagt
     7321 gtagcattca cacaaaactt ccgctatttc accatttata gtttcttggt gtatttctct
     7381 gattatatat ttgggtttgt tttcagtgaa gtattcgcca aggttctttg atgatgatgg
     7441 attgcaaaca tcattaagta tttgatatat aaagccttct atggctctta atgcagagaa
     7501 gcagtatgtt gagtaatctt ccatttcgac atctattttt ttcattatta gcgagcatga
     7561 tagctgtttt ttgatatctt catggatttt atcgatgctt tttggtagtt tgctatgcaa
     7621 ctcggactca atagtttctt tttttatgtc aacattaaat tctttatttt tttgttcgac
     7681 aatctctttc atgtttagta ttgagcacat gaaatcgtta atcaaactcg cgatttgaaa
     7741 ggtattttcc ttggaattga atagagccgc gcttgtaaat ttttgccctg accctgtcac
     7801 cattgctggt ggtcataata tattggtgtt tacaattagg atcgttatta ttatcttctg
     7861 ttattgttat cccctcttca gaaagaaatt caaatagatt tgccctgtca tcatcactga
     7921 attttggaat ggtgtattca aagttctttg tgtctgaata caaacagttt tcttttataa
     7981 tcaaggcgat ttcatcaaag taagtgttat tttgcccaga cgctcttccg atagtggtat
     8041 ttccgcctga tggcattagt tttattaaga agtctattcc tttatatgtg ccagatatgt
     8101 gagtttctct ttcgtttttt acattagagg aatagtttgt gacgccattc tgcgtcagag
     8161 cagactcaat cttgtcaata ttgatattta gtgctttaaa cgggttctgt gccattgggt
     8221 caatccgttg ttttttttga atatgtacag atcttgtttt tttgtcaacg gaata
//
