LOCUS       NC_021730               8721 bp    DNA     circular CON 21-APR-2024
DEFINITION  Acinetobacter baumannii BJAB0868 plasmid p1BJAB0868, complete
            sequence.
ACCESSION   NC_021730
VERSION     NC_021730.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02604246
            Assembly: GCF_000419425.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii BJAB0868
  ORGANISM  Acinetobacter baumannii BJAB0868
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Zhu,L., Yan,Z., Zhang,Z., Zhou,Q., Zhou,J., Wakeland,E.K., Fang,X.,
            Xuan,Z., Shen,D. and Li,Q.Z.
  TITLE     Complete genome analysis of three Acinetobacter baumannii clinical
            isolates in China for insight into the diversification of drug
            resistance elements
  JOURNAL   PLoS One 8 (6), e66584 (2013)
   PUBMED   23826102
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  AUTHORS   Xuan,Z., Zhu,L., Shen,D., Yan,Z., Zhang,Z., Fang,X., Zhou,J. and
            Li,Q.Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2012) Molecular and Cell Biology, University of
            Texas at Dallas, 800 W Campbell Road, Richardson, TX 75080, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: VELVET v. 1.0.18
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000419425.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 01:12:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,843
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,647
            CDSs (with protein)               :: 3,647
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 51 of 99
            Pseudo Genes (incomplete)         :: 37 of 99
            Pseudo Genes (internal stop)      :: 17 of 99
            Pseudo Genes (multiple problems)  :: 6 of 99
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003850.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Acinetobacter baumannii BJAB0868"
                     /mol_type="genomic DNA"
                     /strain="BJAB0868"
                     /db_xref="taxon:1096997"
                     /plasmid="p1BJAB0868"
     gene            join(8680..8721,1..421)
                     /locus_tag="BJAB0868_RS18505"
                     /old_locus_tag="BJAB0868_p0001"
                     /pseudo=""
     CDS             join(8680..8721,1..421)
                     /locus_tag="BJAB0868_RS18505"
                     /old_locus_tag="BJAB0868_p0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /name="DIP1984 family protein"
     gene            complement(744..917)
                     /locus_tag="BJAB0868_RS19985"
     CDS             complement(744..917)
                     /locus_tag="BJAB0868_RS19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(917..1288)
                     /locus_tag="BJAB0868_RS18510"
                     /old_locus_tag="BJAB0868_p0002"
     CDS             complement(917..1288)
                     /locus_tag="BJAB0868_RS18510"
                     /old_locus_tag="BJAB0868_p0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(1560..1814)
                     /locus_tag="BJAB0868_RS20245"
                     /old_locus_tag="BJAB0868_p0003"
     CDS             complement(1560..1814)
                     /locus_tag="BJAB0868_RS20245"
                     /old_locus_tag="BJAB0868_p0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            2487..3437
                     /gene="repM"
                     /locus_tag="BJAB0868_RS18520"
                     /old_locus_tag="BJAB0868_p0005"
     CDS             2487..3437
                     /gene="repM"
                     /locus_tag="BJAB0868_RS18520"
                     /old_locus_tag="BJAB0868_p0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            3430..4005
                     /locus_tag="BJAB0868_RS18525"
                     /old_locus_tag="BJAB0868_p0006"
     CDS             3430..4005
                     /locus_tag="BJAB0868_RS18525"
                     /old_locus_tag="BJAB0868_p0006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(4357..4875)
                     /locus_tag="BJAB0868_RS18530"
                     /old_locus_tag="BJAB0868_p0007"
     CDS             complement(4357..4875)
                     /locus_tag="BJAB0868_RS18530"
                     /old_locus_tag="BJAB0868_p0007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            5248..5535
                     /locus_tag="BJAB0868_RS18535"
                     /old_locus_tag="BJAB0868_p0008"
     CDS             5248..5535
                     /locus_tag="BJAB0868_RS18535"
                     /old_locus_tag="BJAB0868_p0008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            5522..5836
                     /locus_tag="BJAB0868_RS18540"
                     /old_locus_tag="BJAB0868_p0009"
     CDS             5522..5836
                     /locus_tag="BJAB0868_RS18540"
                     /old_locus_tag="BJAB0868_p0009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            5964..8375
                     /gene="znuD2"
                     /locus_tag="BJAB0868_RS18545"
                     /old_locus_tag="BJAB0868_p0010"
     CDS             5964..8375
                     /gene="znuD2"
                     /locus_tag="BJAB0868_RS18545"
                     /old_locus_tag="BJAB0868_p0010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     Typing          2488..3437
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaaaaatt atttcactaa aacaacgtat caatgcaaat gtattggtac aagatggtga
       61 tcaaccatct gaagatccaa atgaactact aaaacaagta ttttctttaa ttcaagaatt
      121 tcaaaaatta agttatgcaa ttcatgaaac taatgcttta acaaaattaa atgatggccg
      181 ttctctactt gctttattga ctttgcgtga tgaatttgta gaacagcaca aaaccctaac
      241 tgcagcaata agtaatacat ctcgtgaatc tgatcgttat agtacgcggg aaataaagtg
      301 gcataaagtt attccagttt catcattaca aaaacaagct gatgatatta gtcttaaatt
      361 acgtgattta aacgtgttaa ttcagtcgac taattggaaa attgatttga ttgaagccta
      421 atagtccttt agatataatc aagttgaaca tactgggcga gtacaagatc ccttacttcc
      481 acaacatagg attgtaaata aagtgtagtg gcttgcaaag caagtggata agcttaaaat
      541 tttaagtaca aatcaatcca tcacttggca taactcaaat ctttgtgcaa acaaagttac
      601 acgttactta taactaccat gtactgacag tatgttcacc tcaattacta aaaataaaga
      661 aatgaacaag catcagctca actacatttc tatttttaat tcagttcggc atttaacata
      721 aaatctctta tacgaagccc aggttaattc tgggcttttt gttcaatctc atcattggcc
      781 gagaacatac ttggattcgt aagcctgata ttttcctgac aacttccaca cagcatcaat
      841 cctgcaaaaa gttgtacatt ttgtctattt tcacaaagcg tacatggctt tgaaagctga
      901 ataatattat ctgacactac tttaccctct tatagttatc atctgcatat gaataacctt
      961 tagcttgtat cattctaacc tcatctttag aagcgccatt tttcattaag agccacaaca
     1021 tcatgaaata tagtccagaa aagtcttcat agtatttttc agaaaaattg ttatcttcct
     1081 caaaatcatc tattcgcttt tctaatcgat tcaccagatc ctcaatacga tcggcttgct
     1141 gtttaacagc aatagattga aaatcattgg tcaaaatttt actgaataat ttactggttc
     1201 tgattccttc tgaattagcc aattgatcgt agaattctgc tacttgtgga tctaacctga
     1261 ctgcataagt tttgactttt tcgcccataa attacacctc ttattaaaat tagattttgt
     1321 acaaaattaa atatatataa ttcaactaat taaataaaaa ataataatta gattacgtac
     1381 aaacttattt gtacagaatt aatttattct aaaacaaaaa cttatttaaa tataatgctt
     1441 tttttatgta caatcatgag tacatatttt ttcttaaatt tatcaatatc ttatctaaaa
     1501 aaagtgttgt tttgatgtac aaccatgagc acagcataaa aaatagataa ttcaatcact
     1561 tattctaaaa aaagaccatt tttctgtaca cgtttctgag accctaaaaa atcgctgaaa
     1621 tccttatcct gtaaggcttt gaggggtgtc atcgttagat gcgtggtgtg tgacaaaaaa
     1681 atcccggcat gtgccggatc ttggattgga aaattggcta aagtgacgta gggccggtgc
     1741 ttggttttgc atggaaaaaa gtatttattt ccttgtttag aaaaacgtaa aaaatcaatt
     1801 ttttgtccat tcatccaggt taaaaatttc aacccaaaac tttaattatg aaaagcttca
     1861 tagaacgcgt tcaaatgcga tttaagagcc tttacctaga aaacatagat ctcatagtga
     1921 aaaacacaaa acagctcaaa aaacgcaaat gagagcgaaa taaagaggta ttttgacttt
     1981 agatagatgc atagagcgag tgtctacgag cgaactatca aaatttgcgc ctagaccctc
     2041 tgaaaaacat ttttttgtcc tctttagcct aagaaagcgt aagtttcgtg cagaaatttg
     2101 ctcctggatc gagcgtagcg agtaaaaaag ttttatgagc gaagcgaatt ccaagtttct
     2161 tttgcttttt tttaaagtca cgccagtatt aaccaaaaaa ttgccccaac gaactgagcg
     2221 aaagcgaagt tcaatagagt ttgagcgaag cgaaaaccaa gggcaatttt tcattccctg
     2281 ggcttttaat tattttaaag tttttaaata cttttaaatg gcttgaaagc cttatgaata
     2341 aagggtttta gctcttatat gtccacgttt accttgcaat atgtccacgt ttaccttgca
     2401 atatgtccac gtttaccttg caattacctt gcattagtac acaaataata ttaacgtgta
     2461 cttatacaca ataaaaaata gtggctatga gagatttagt tgtaaaggac aatgccttaa
     2521 tcaacgcaag ctataactta gacttagtag aacaacgttt aattttattg gctattgttg
     2581 aagcaaggga aagtgggaaa gggattaatg caaatgatcc attagaagtt catgcagata
     2641 gttatatcaa tcaatttggt gtacaccgta atacagctta tcaagcctta aaagatgctt
     2701 gtaaggattt attcgcgcgt caatttagct atcaagagaa aaaagctaat gggaatatcc
     2761 gaaatgttat gagtcgttgg gtatctcaaa ttgcttataa cgacaatgaa gcaactgtag
     2821 acttaatatt tgcacctgct gttgttccct tcataacccg actggaagaa caatttacta
     2881 aatatgaatt acagcaagtt agtagtctta gtagcgctta tgccattcgc ttatatgagc
     2941 ttctaattca gtggcgaagc actggtaaaa ccccaaccat agaactacaa gaatttagaa
     3001 agaagttagg cgttcttgat aatgaatatt tacggatggc tcatttaaaa gagcgtgttt
     3061 tagagctttc aattaaacaa ataaatgagc atacggatat aactgtaaaa tatgaacagc
     3121 ataaaagagg acgttctatt tcaggatttt cttttacctt taaacagaag aagaaggata
     3181 gcccatcaat agaaagagat ccgaacactt tggagctttt ttcaaagatg accgatgctc
     3241 aacggcatat gtttgcaaat aaactttcag aactccctga aatgggtcgc tattcacaag
     3301 gaacagaaag ctaccaacag tttgctgtac gtattgctga gatgctacaa gatcccgctc
     3361 aattcaaaga gctataccca tacctaaaaa aagtgggata catgccatca aataaaaagg
     3421 acaccgtaaa tggctaagtt atcactaagt gaagtatcta aaaaatttca tgtggataga
     3481 tcaaccattt acagagctgt acgtaatgga cgtttatcac gctccagtga tggacaattc
     3541 gatctagcag aggtcatacg atgctttgga gaacctgagc aaacatctca aaaaattgaa
     3601 tcatctaagc aagaaggtga tgaatctaca aaaaaactta ttgcccattt agaaaatgaa
     3661 gtcaaaaaat accaagaacg tgaagaacgg ttaatgcaac aaattgaccg tatgcaaaca
     3721 ctcattgagc tgaaaagtgt tgcacctgcc acagcagcac cacaccaaga tgctacggca
     3781 tgcgacacca agatgccaca gcatgcgaca acacaacaag acactgataa caaaaagaat
     3841 aatgaattaa atattgcaga aaatgtagca gtgccacagc aagaaactac ggcataccac
     3901 acccaaacgc tacagcatgc cacgttgcaa agtgtggcag tgccacaaca caaaaaacgt
     3961 ggtttatttg gccgtgtgct gaatgccgtc tttgataatg actgagcaat aagggtgaaa
     4021 gatcatgccg aaactgaaag acattgccct gggaattatt gtagctccgc tgctgatccc
     4081 gatcatgctg atttgcatcg taccaggata aaaaggcgct aagaaagagc tggatgaatg
     4141 ccaaaaagaa aaagaccagg catcctgatt attcaattga gctaatcaaa agtgtggaac
     4201 attgatgaaa taatagcgtg gaacgtgaaa agtcataaaa aaagcccgaa cagggatgct
     4261 cgggctataa actagaaact acagcgttga attataagga taattataca gcatttcgta
     4321 taaggtgtat tatgttaatt ttaggaaata ttaaaactaa ttttcagact tatcccacca
     4381 actttgagct aaatcagtat ttttaggaat acctatacca tcacgataca taacggaaag
     4441 acttcgcatt gctttcttat tacctaactt tgcagccttt tcacgaagtt caaaagcttt
     4501 attaatatta acttctattc cgctatctgc acgagtatat agatcagcta actcaaccat
     4561 tgattcagaa tggccatttt ctgaggcttt ctgaaaccac ttaaaagctt tttggaaatg
     4621 ttctaaagaa tcgtttttgt catcatcttc cattaatcta gcataagtaa catatccatc
     4681 agctaaccaa aacattgctg ctacatttcc attttctgct gatttaagag cccaaacttc
     4741 agcttgattt tcatctgaat caacaccatg accatataga taaatctcgg ctaattctaa
     4801 ctgagcagaa gtatctccat tttctgcttt cgctaataaa tcattagaaa aaacaggttc
     4861 tgcaaatgtt tccattgaaa ggcaaactcc taaagccata aaaatatttg ttaatttatt
     4921 caatttttaa cctttaaact aaactgttag gcttgtatat ttttatatca tacctaaaag
     4981 aaaactctaa aattagagta tgtcttatct ctatttaaca taatggcgat tatacgaagt
     5041 acacttgtat attaatataa atatcaacta cttaataaaa atagaaaaat cccaaaatgc
     5101 ccaaaaagcc gactttgaaa caagtcggct tttttatgag cagttttgat acttcttgcc
     5161 aataattttg actaaaaatt acacgaatct ggttgagtga tacaattttg tgatacatta
     5221 ttgtatatac aaatttgaat gttttttatg gaacagtatt ttgaatggga tgaggctaag
     5281 aatcgaaaga atcagaaaaa gcatgatatc tctttcgaaa ctgcaagcct tgtttttgaa
     5341 gatccattac ggatctcaat ccaagacaga cataccaatg gtgaagaacg ttggcaaacc
     5401 attggacgag ttaaaggcgt actaatgtta ttggtggctc acaccatttt tgatgaagat
     5461 gactgtgaaa tcatccgaat cattagtgca aggcaagtca ctaaagcgga gcgaaacaaa
     5521 tatgagcatg gttagatact cacacaaaga gctgaatgag aaatttggtg agaagcaaga
     5581 tgctgaaatt caacgcttgc ttgctaaagg cacggttcct gatgatcagc tagatctatc
     5641 agatattcct gaaattacag actggagcaa tgctgtacgc caaaatcaat tttaccgccc
     5701 agtaaagcaa caaacttcaa ttcggttaga tgctgatgta cttgcttggt tcaaagcaca
     5761 aggcaaaggt tatcaaacac gtatgaatga aattttacga gatgctatgc taaaagaatt
     5821 aaaaaatcat caataagaat gggagcttag gctcccattt ttgacttcat ttttaggggg
     5881 gattaccatt caaaaattta aatagtagat tttataaatt tttaagtgtt atattataat
     5941 ataacattaa atttggttgt tttatgctaa ataaaagtaa actattttta gctttaatta
     6001 ctttaggggc ctcgaaaatt ttattggcag ctgaagggcc tgttactacc ttaaatacaa
     6061 tagtattaac tgcacaatct gatgagttag ggtctgagct gttaggcaaa agtctaaatg
     6121 ttagtaatca gtttattgat actagtaaac ttaaacagcg ttcgacaact ctaggggatg
     6181 ccttgggtac ggaattagga attcattcta atcaatatgg aggtggagca tctactccta
     6241 ttattcgtgg gcaggaagga aaacgtatta aagttttaca gaataatgct gacgtgttgg
     6301 atatgtctaa tatgtcccca gatcacgctg tcacagttga accaagttta gctaagtcta
     6361 ttgaaattat acgtggtgca agtacccttt tgtatagttc aaatagtgcg gcaggggtag
     6421 tcaatgtcat tgactataaa attccaaccc aaatgcctca agacggatta gagggtaata
     6481 ctacattacg ttttaatact ggcagtaatg agaagttgac cacagcaggt gtaacagttg
     6541 gcttaagccc acatgtagct ttacgggcag aagggttata tcgtaatgct ggtaactata
     6601 aaactcctca ttaccagtct agctcttata attctttaga agatcttgaa aaccaaaaca
     6661 gtatttataa aaatttaaaa tatttaccag aaagctgggc agagtcgaga gtaggtacgc
     6721 ttggattatc ctggatagac gataatactt atttaggagt ttcttataca catcgtcatg
     6781 atgaatatgg tttaccagcc catagccatt tatatgaagg ttgtggtgcc tctgcaatcg
     6841 gtatcgattc acgaattagt ggattaaaaa attatctttt atattatcct caattgatgg
     6901 atgagcaaga tattaattac ataaatccac gtcctgattg tcatcagcat aatcatattc
     6961 atgaaactaa tttctctcat aatgctcctt atattgattt aaatactcga cgttatgatg
     7021 tgcgtggaga attcactcaa ccatttaccg gtattgacaa aattcgtacc tcattgagtt
     7081 atatagatta ttttcacaat gaactcgagg gagataagat tactaacttt tttaaaaata
     7141 caggaaaagt aggccgcata gagttatcac atcagccatt aggggaatta accggtatct
     7201 tggggctaca atatttggaa caggataata gtgctttatc accagtccat tctcaagaag
     7261 gccataccac ctatctagat aatcagcagc tattaaatcg aaatgtcact aaaaacttta
     7321 gtgtatttgg cctagaaaaa tataactgga acgactttac tttcgaacta ggtgctcgta
     7381 tagaaaagca gaaagttagt atggattatg atatagaaaa aattaaagat tcaatgaaac
     7441 cttggcctaa taaatataac agtccttatg tagaaaaaaa taataaaatc agggctcaaa
     7501 atttaaaatc tattttagaa gctgttcaac ctaacaaaga aacagctttt tcttatgcag
     7561 gtacagtcca ctggagattt gctccaaatt atatcttatc attaacaggt acacatcaag
     7621 aaagattacc taatgcacaa gaaatgtata ctcatggtat gcatctcgct actaattctt
     7681 ttgaaatagg taaccgcttt ctaagaaaag agaaatccaa taatttagaa atcagtctgg
     7741 cctataagga tgatttactt gattatcaaa tcagtactta ctattatgat tttgataact
     7801 atatatattt acagacactc aatgaagttt taggaactac aaaagttaga gatcagcata
     7861 cactacgtat taatcattat agccagtctg ctgctaattt ttatggatta gagggcaaca
     7921 tagggtatca atttaattct gtttatcacg gctctctttt tggagattat gtgaaaggcc
     7981 gtttaacaaa tttacctgat gctgtaattg catacgatat ctggaataga gaaccgactt
     8041 tagctcccca aaaggatcgt tacactccta gattacctcc tgctcgtttg ggtacacgac
     8101 taaaagctga ttttgatgaa tcgcttaaag gtgaaataga atattatcga gtctttaaac
     8161 aagataatat ttctaaattt gagcaggtta catcaggtta taatatgctt aatatgactt
     8221 tagcatataa aaataagcta tctcacacag aatatgattt attctttaaa gcgaataatt
     8281 tacttgatca gaaagtctat gcccatgaaa catttttgcc atatattccc caaattggaa
     8341 gaaattttag tttaggctta aatcttaatt tctagataag atactgtaat cttctaagaa
     8401 aatttcttag aagatttttc ttataaaatg attagattaa taattaaggt aagcgtccgc
     8461 tgaatcccat acctattttt taattcgatt taggtttcca gcagtgtggc agtaactctt
     8521 caatctgggt cactttatgt gtcggcagcc ttttcagcac tgaatagcca ccgattttgt
     8581 agacaccttt tagctagata aaaagcaatt tttttgatct ttaaagaatg aggatataag
     8641 ctgtttaaaa cttaattcta attggttaat aggtgaaaaa tgaaattagc agaagctctc
     8701 ttacttcgaa gtgatcaaca a
//
