LOCUS       NC_021657               2908 bp    DNA     circular CON 14-DEC-2023
DEFINITION  Staphylococcus aureus plasmid pBmb9393, complete sequence.
ACCESSION   NC_021657
VERSION     NC_021657.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02603524
            Assembly: GCF_000418345.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Costa,M.O., Beltrame,C.O., Ferreira,F.A., Botelho,A.M., Lima,N.C.,
            Souza,R.C., de Almeida,L.G., Vasconcelos,A.T., Nicolas,M.F. and
            Figueiredo,A.M.
  TITLE     Complete Genome Sequence of a Variant of the Methicillin-Resistant
            Staphylococcus aureus ST239 Lineage, Strain BMB9393, Displaying
            Superior Ability To Accumulate ica-Independent Biofilm
  JOURNAL   Genome Announc 1 (4), e00576-13 (2013)
   PUBMED   23929475
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Vasconcelos,A.T.R., Motta,C. and Almeida,L.G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-AUG-2016) Labinfo, LNCC - Laboratorio Nacional de
            Computacao Cientifica, Rua Getulio Vargas 333, Petropolis, RJ
            25651070, Brazil
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 2908)
  AUTHORS   Costa,M.O.C., Beltrame,C.O., Lima,N.C.B., Almeida,L.G.P.,
            Vasconcelos,A.T.R., Ferreira,F.A., Nicolas,M.F. and
            Figueiredo,A.M.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2013) Labinfo, LNCC - Laboratorio Nacional de
            Computacao Cientifica, Rua Getulio Vargas 333, Petropolis, RJ
            25651070, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Newbler v. 2.5.3; Celera software v.7
            Genome Coverage                   :: 25x
            Sequencing Technology             :: 454 GS FLX Titanium
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/14/2023 01:50:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,014
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,825
            CDSs (with protein)               :: 2,825
            Genes (RNA)                       :: 80
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 69 of 109
            Pseudo Genes (incomplete)         :: 35 of 109
            Pseudo Genes (internal stop)      :: 23 of 109
            Pseudo Genes (multiple problems)  :: 17 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP005289.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Bmb9393"
                     /db_xref="taxon:1280"
                     /plasmid="pBmb9393"
     gene            join(2846..2908,1..657)
                     /gene="catA"
                     /locus_tag="SABB_RS15360"
                     /old_locus_tag="SABB_06321"
                     /pseudo=""
     ARG             2..585
                     /name="catA"
                     /gene="catA"
                     /gene_name="type A-9 chloramphenicol O-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="100.0"
                     /coverage="90.28"
     gene            complement(1453..2376)
                     /locus_tag="SABB_RS15365"
                     /old_locus_tag="SABB_04884"
                     /old_locus_tag="SABB_06322"
     CDS             complement(1453..2376)
                     /locus_tag="SABB_RS15365"
                     /old_locus_tag="SABB_04884"
                     /old_locus_tag="SABB_06322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499696.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_000417606.1"
                     /translation="MEKYTEKKQRNQVFQKFIKRHIGENQMDLVEDCNTFLSFVADKTL
                     EKQKLYKANSCKNRFCPVCAWRKARKDALGLSLMMQYIKQQEKKEFIFLTLTTPNVMSD
                     ELENEIKRYNNSFRKLIKRKKVGSVIKGYVRKLEITYNKKRDDYNPHFHVLIAVNKSYF
                     TDKRYYISQQEWLDLWRDVTGISEITQVQVQKIRQNNNKELYEMAKYSGKDSDYLINQK
                     VFDAFYKSLKGKQVLVYSGLFKEAKKKLKNGDLDYLKEIDPTEYIYQIFYIWKQKEYLA
                     SELYDLTEQEKREINHKMIDEIEEEQ"
                     /name="protein rep"
     ARG             2..588
                     /name="Streptococcus suis chloramphenicol
                     acetyltransferase"
                     /gene="Streptococcus suis chloramphenicol
                     acetyltransferase"
                     /gene_name="chloramphenicol acetyltransferase (CAT)"
                     /tool="rgi"
                     /drug_class="phenicol antibiotic"
                     /identity="94.36"
                     /coverage="90.28"
     ARG             2847..2908
                     /name="Limosilactobacillus reuteri cat-TC"
                     /gene="Limosilactobacillus reuteri cat-TC"
                     /gene_name="chloramphenicol acetyltransferase (CAT)"
                     /tool="rgi"
                     /drug_class="phenicol antibiotic"
                     /identity="100.0"
                     /coverage="8.82"
     Typing          complement(1454..2388)
                     /name="rep_cluster_1230"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaccaacaaa cgacttttag tataaccaca gaaattgata ttagtgtttt ataccgaaac
       61 ataaaacaag aaggatataa attttaccct gcatttattt tcttagtgac aagggtgata
      121 aactcaaata cagcttttag aactggttac aatagcgacg gagagttagg ttattgggat
      181 aagttagagc cactttatac aatttttgat ggtgtatcta aaacattctc tggtatttgg
      241 actcctgtaa agaatgactt caaagagttt tatgatttat acctttctga tgtagagaaa
      301 tataatggtt cggggaaatt gtttcccaaa acacctatac ctgaaaatgc tttttctctt
      361 tctattattc catggacttc atttactggg tttaacttaa atatcaataa taatagtaat
      421 taccttctac ccattattac agcaggaaaa ttcattaata aaggtaattc aatatattta
      481 ccgctatctt tacaggtaca tcattctgtt tgtgatggtt atcatgcagg attgtttatg
      541 aactctattc aggaattgtc agataggcct aatgactggc ttttataata tgagataatg
      601 ccgactgtac tttttacagt cggttttcta atgtcactaa cctgccccgt tagttgaaga
      661 aggtttttat attacagctc cagatccata tccttctttt tctgaaccga cttctccttt
      721 ttcgcttctt tattccaatt gctttattga cgttgagcct cggaaccctt aacaatccca
      781 aaacttgtcg aatggtcggc ttaatagctc acgctatgcc gacattcgtc tgcaagttta
      841 gttaagggtt cttctcaacg cacaataaat tttctcggca taaatgcgtg gtctaatttt
      901 tatttttaat aaccttgata gcaaaaaatg ccattccaat acaaaaccac atacctataa
      961 tcgataacca cataacagtc ataaaaccac tcctttttaa caaactttat cacaagaaat
     1021 atttaaattt taaatgcctt tattttgaat tttaaggggc attttaaaga tttaggggta
     1081 aatcatatag ttttatgcct aaaaacctac agaagctttt aaaaagcaaa tatgagccaa
     1141 ataaatatat tctaattcta caaacaaaaa tttgagcaaa ttcagtgtcg attttttaag
     1201 acactgccca gttacatgca aattaaaatt ttcatgattt tttatagttc ctaacagggt
     1261 taaaatttgt ataacgaaag tataatgttt atataacgtt agtataataa agcattttaa
     1321 cattatactt ttgataatcg tttatcgtcg tcatcacaat aacttttaaa atactcgtgc
     1381 ataattcaac agctgacctc ccaataacta catggtgtta tcgggaggtc agctgttagc
     1441 acttatattt tgttattgtt cttcctcgat ttcgtctatc attttgtgat taatttctct
     1501 tttttcttgt tctgttaagt cataaagttc actagctaaa tactcttttt gtttccaaat
     1561 ataaaaaatt tgatagatat attcggttgg atcaatttct tttaagtaat ctaaatcccc
     1621 attttttaat ttctttttag cctctttaaa taatcctgaa taaactaata cctgtttacc
     1681 tttaagtgat ttataaaatg catcaaagac tttttgattt attaaataat cactatcttt
     1741 accagaatac ttagccattt catataattc tttattatta ttttgtctta ttttttgaac
     1801 ttgaacttgt gttatttctg aaatgcccgt tacatcacgc cataaatcta accattcttg
     1861 ttggctaata taatatcttt tatctgtgaa atacgattta tttactgcaa ttaacacatg
     1921 aaaatgagga ttataatcat ctcttttttt attatatgta atctctaact tacgaacata
     1981 tccctttata acactaccta ctttttttct ctttataagt tttctaaaag aattattata
     2041 acgttttatt tcattttcta attcatcact cattacatta ggtgtagtca aagttaaaaa
     2101 gataaactcc tttttctctt gctgcttaat atattgcatc atcaaagata aacccaatgc
     2161 atcttttcta gcttttctcc aagcacagac aggacaaaat cgatttttac aagaattagc
     2221 tttatataat ttctgttttt ctaaagtttt atcagctaca aaagacagaa atgtattgca
     2281 atcttcaact aaatccattt gattctctcc aatatgacgt ttaataaatt tctgaaatac
     2341 ttgatttctt tgttttttct cagtatactt ttccatgtta taacacataa aaacaactta
     2401 gttttcacaa actatgacaa taaaaaaagt tgctttttcc cctttctatg tatgtttttt
     2461 actagtcatt taaaacgata cattaatagg tacgaaaaag caactttttt tgcgcttaaa
     2521 accagtcata ccaataactt aagggtaact agcctcgccg gcaatagtta cccttattat
     2581 caagataaga aagaaaagga tttttcgcta cgctcaaatc ctttaaaaaa acacaaaaga
     2641 ccacattttt taatgtggtc ttttattctt caactaaagc acccattagt tcaacaaacg
     2701 aaaattggat aaagtgggat atttttaaaa tatatattta tgttacagta atattgactt
     2761 ttaaaaaagg attgattcta atgaagaaag cagacaagta agcctcctaa attcacttta
     2821 gataaaaatt taggaggcat atcaaatgaa ctttaataaa attgatttag acaattggaa
     2881 gagaaaagag atatttaatc attatttg
//
