LOCUS       NC_021523               4269 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli plasmid pCGB40, complete sequence.
ACCESSION   NC_021523
VERSION     NC_021523.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226289
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Zhang,S., Sun,J., Liao,X.P., Hu,Q.J., Liu,B.T., Fang,L.X., Deng,H.,
            Ma,J., Xiao,X., Zhu,H.Q. and Liu,Y.H.
  TITLE     Prevalence and plasmid characterization of the qnrD determinant in
            Enterobacteriaceae isolated from animals, retail meat products, and
            humans
  JOURNAL   Microb Drug Resist 19 (4), 331-335 (2013)
   PUBMED   23557071
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Zhang,S., Sun,J. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2012) South China Agricultural University, College
            of Veterinary Medicine, Wushan, Guangzhou, Guangdong 510642, China
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009959255.2-RS_2024_06_25
            Annotation Date              :: 06/25/2024 01:53:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JQ776504.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CGB40"
                     /isolation_source="pigeon feces"
                     /db_xref="taxon:562"
                     /plasmid="pCGB40"
                     /geo_loc_name="China"
                     /collection_date="2010-06-13"
                     /collected_by="Yang Wang"
                     /PCR_primers="fwd_name: invdr/f, fwd_seq:
                     caggcgcttcagcttgtt, fwd_name: qnrd-f, fwd_seq:
                     cgagatcaatttacggggaata, rev_name: invdf/r, rev_seq:
                     tattccccgtaaattgatctcg, rev_name: qnrd-r, rev_seq:
                     aacaagctgaagcgcctg"
     gene            554..1198
                     /locus_tag="HUF31_RS00005"
                     /old_locus_tag="D616_p102001"
     CDS             554..1198
                     /locus_tag="HUF31_RS00005"
                     /old_locus_tag="D616_p102001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrD1"
                     /protein_id="WP_012634451.1"
                     /translation="MEKHFINEKFSRDQFTGNRVKNIAFSNCDFSGVDLTDTEFVDCSF
                     YDRNSLEGCDFNRAKLKNASFKSCDLSMSNFKNISALGLEISECLAQGADFRGANFMNM
                     ITTRSWFCSAYITKTNLSYANFSRVILEKCELWENRWNGTVITGAVFRGSDLSCGEFSS
                     FDWSLADFTGCDLTGGALGELDARRINLDGVKLDGEQALQLVESLGVIVHR"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrD1"
     gene            complement(1819..2205)
                     /locus_tag="HUF31_RS00010"
     CDS             complement(1819..2205)
                     /locus_tag="HUF31_RS00010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058671329.1"
                     /translation="MRISNVEWLKKRIGFIRKLGEKTTRQRQIIDLLDDEASLSETDRR
                     LLHVLATAEKNELQEQDAAQKKAVQARISGQKNRRERNHKLFLAAGLMIDAGLVDTKTG
                     ELNFDSEKLLTALKRVRCDLETSV"
                     /name="hypothetical protein"
     gene            2373..3308
                     /locus_tag="HUF31_RS00015"
     CDS             2373..3308
                     /locus_tag="HUF31_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_173479971.1"
                     /translation="MATYHLSVKFGGKGKALAHANYITREDKFSQRKDLEHTEHGNMPE
                     WAKDDPAHFWQAADAFERANGSTYREVEIALPRELNEAQRLELVRDFVKQETGGQHTYS
                     FAIHNPQASIDGGEQPHAHIMMSQRVDDGIKRSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKAQGIDRKPEQHLGFEAAGR
                     LDDSQRQDIKDSRTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQY
                     GREMAARVTKQGNVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     gene            3311..3841
                     /locus_tag="HUF31_RS00020"
     CDS             3311..3841
                     /locus_tag="HUF31_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     ARG             555..1195
                     /name="qnrD1"
                     /gene="qnrD1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrD1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          305..460
                     /name="Col3M"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.089"
                     /coverage="100.0"
                     /evalue="2.29e-71"
     Typing          2374..3305
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcactcggt tcgcggggct acagcgtttc tgtgtccgaa accgaacgat tacgtacagc
       61 atgtagacaa catgtccgaa agtaagcaaa accattaacg gacagccgga tcatttcgta
      121 catcccttac ggtcaggggt tcgcgctcaa tggaacgaaa tcacccgtta gcttaaaacg
      181 cttaacgtag gataatttcc tttttccaag cggacccgta ctcggttggt caaatcgctt
      241 cttgccacaa aacaagtttg tgtctttcag cgatttcgca tcgttttact tgtcagtttg
      301 aatgatgtta aaaacgcact cagaagctcg caaaaccgtt ctaaactttc agcgtagatt
      361 tgggtgtttt atagggtgat agtgtcttaa aatcgttttg aggggcttta gtggggatga
      421 acgggcggtt ttcgctaact aactcgcccg tttaacataa aagggattgt atgaatcaat
      481 tttagctaga gtttaaggtt gttcaaatta atgtacaatg attgcactgt ataaataacc
      541 aggtgtagca tgtatggaaa agcactttat caatgaaaag ttttcacgag atcaatttac
      601 ggggaataga gttaaaaata ttgccttttc aaattgtgat ttttcagggg ttgatttaac
      661 tgatactgaa tttgttgatt gtagttttta cgacaggaat agcttggaag ggtgtgattt
      721 taatagagcc aaactaaaaa acgctagctt taaaagctgc gatttatcaa tgagtaattt
      781 taaaaacatt agcgccttag gtcttgaaat tagtgagtgt ttagctcaag gagctgattt
      841 tcgaggggct aattttatga atatgataac tacaaggtca tggttttgta gtgcttatat
      901 aaccaagaca aatcttagtt acgctaattt ttctagagtc atattagaaa agtgcgaact
      961 gtgggaaaat cgctggaatg gcactgtgat aactggcgcc gtgtttcgtg gctccgatct
     1021 ttcttgtggg gagttttcat cgtttgattg gtctttggct gattttactg gttgtgattt
     1081 aacgggtggg gcgcttggcg agcttgatgc aaggcgaatt aatttagatg gagtgaagtt
     1141 ggatggagag caggcgcttc agcttgttga gagtttaggt gttattgttc accgataaaa
     1201 tctaggtaaa aaacgcctaa tgccccaatg tggtactaat caaaacggag cgaagcgaca
     1261 tcttcatctt gtctttgctt ttctccatga tcagttattg ggtcatggtc tcttagctcg
     1321 ttaatctggc taagtttggt taggttgggc ttctttcgac agttgcaatc caagaaatcc
     1381 ctagtccgtt gattggtgct cttatcaggt acgcactgta atctttcgat accgcagcgt
     1441 gattcggagg cagagccgga atttcgtttt ccggtacgtt ttactaccac aatttgcctg
     1501 tttatccttt cggagaccgg gctaagtccg agtcaatcaa gtggtgcatt tgagttaatg
     1561 tcgttgaatg gttttaagga agtagtgaca agctagcttc ccattgctaa gattggattg
     1621 tctaggtctg atcttagcaa cgaatgcacc gaatcagatt agcagagccg gagtgatgtc
     1681 acaatcattt cggctctgcg ctttctgaac ttaagcaaaa aatcccctcg tatttcaatc
     1741 tgcaacgctc gccgcaagat gcaaactggt tttgcatcga ggaagcgtca tatcactgaa
     1801 tccttgcaga atgcacggtc acaccgatgt ttccagatca cagcgtaccc gtttcagcgc
     1861 ggtcagtaat ttttcagagt caaaattcaa ctcacccgtt tttgtatcaa ccagtcccgc
     1921 gtcaatcatc agtccggcag ccaagaacag tttgtgatta cgttccctgc ggtttttctg
     1981 acctgaaatc cgtgcctgaa ccgccttttt ctgcgcggca tcctgttcct gcaactcgtt
     2041 tttttcagcg gttgccagaa cgtggagcag tcgcctgtca gtctcactga gcgaagcctc
     2101 atcatccagc aggtcgataa tctgccgttg ccgtgttgtt ttctccccca gttttctgat
     2161 gaagcctatg cgctttttaa gccattcgac gttactgatc cgcatcgttt aacttcccca
     2221 tcccgatatt tccgatggtg aaagtatata acttgcggtc agaagtcgca tgacttatct
     2281 ttactcataa ttcttcaaat tagagtaaag cgcacttata tgtttctccc tgcgggcaga
     2341 aacacgtgcg taaaaccaag ggcaaaaaca tcatggcaac ctatcatctc agcgtgaaat
     2401 ttggcggcaa gggtaaagcc cttgctcatg ccaattacat cacgcgggaa gataagttca
     2461 gtcagcgaaa agatctcgaa cacaccgagc acggcaatat gccggaatgg gcgaaagatg
     2521 accccgccca tttctggcag gctgcggatg cgttcgaacg tgccaacggt tcaacgtacc
     2581 gggaagtcga gatagcacta ccccgtgaac tgaacgaagc gcagcggctg gagcttgtca
     2641 gggattttgt gaagcaggaa acgggcggtc aacatacgta ttcgtttgcg attcacaatc
     2701 cgcaagccag cattgacggc ggcgaacagc cgcacgccca tatcatgatg agccagcgtg
     2761 ttgatgacgg tattaagcgc agcccagagc agtatttcag gcgctataac gccaaatttc
     2821 cggcgcgtgg cggcgcgcgc aaggactcag ggcataattt aacccctaca gaacaaaaac
     2881 aggcactgaa agccctgcgg gagcgctggg agcgtacgca caacgcgcat atgaaacgat
     2941 atggctttga gcagggtttt attgatagcc ggtcactgaa agcacagggt atagaccgga
     3001 aaccggaaca gcatctcggt tttgaagcag ccggacgact ggacgacagc cagaggcagg
     3061 atattaaaga cagccggacg ttttcagtcg tggatgtgcc tgaacaggcg aagcatatcg
     3121 accacagcga ccgtagctgg atgaatgcca gcggtatcga ggtaaaccgg aaggaactgg
     3181 tcagatctct gacgcatgat atctcgcagc agtacgggcg ggagatggct gcacgggtca
     3241 caaaacaggg aaatgttgaa atagagcgcg tgaaatccct gtcacgcgga ttttccataa
     3301 gcaggtaact atggcgagca ccgaagaaaa acttctcaca ctcctgctgt cggcggagca
     3361 tctgaaagag gccgcaaaag agcagcagaa agccgtggcg gattcgctgg cggtggcaag
     3421 ggaaaccctg aaaggctacc gggaaatatc agacagtgtg gcacagcagg tacgggatga
     3481 agtccggcag gaagtgcaga aaatggatgt tgcggggctg attggcgagc gtatcagcgc
     3541 tgattttcag cgtctggaaa aatccgtcgg gaacatgaaa atcaatgcgg acgcgctgga
     3601 caaggaaatt tccattatcc gcagcaatat catcgcggag tacaacacgc tgcggggtta
     3661 cagctggaag tggtttgcag gggttgcggt tgcctttgtc gtactgatat ttgccacaag
     3721 ctggtttgtg aaaacggaac tacaggacaa ttacaacgcg tctgctgccg tctatcagaa
     3781 agtggcagac cttgaaaagc tgctgacaac ggaacagaaa ccggaaaaga aaaagaactg
     3841 acgcgctgcg cttgtccaac gcctcacgtt cgcacaataa attgcgcacc cgttcaccgt
     3901 tttcagccgg gtttggggcg tcttttgaga cttccggcct gaattcaggc cggaagttct
     3961 cgccagtcag tcaggtgcat ggttatgcat ggggctgaaa atcacggtat gcgattctga
     4021 gcggttttcg gtgagaagac gggggttttt atggctcagg acgtaaaacc gccctgagcg
     4081 cgtttcagcg gtgcgcgtaa tgacgcgtta tggtaaatcg gtgccacagg cgtaagtatc
     4141 tgctaaggat ctttttgttg attctttttt aagagcataa tgagattata taaattgatt
     4201 ttggggtccg cttggaaaag ggaaaataac ctacgctaag actacttttt aaccaatcgg
     4261 ctattggat
//
