LOCUS       NC_021522               4268 bp    DNA     circular CON 03-JAN-2024
DEFINITION  Citrobacter freundii plasmid pCGF41, complete sequence.
ACCESSION   NC_021522
VERSION     NC_021522.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226288
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Zhang,S., Sun,J., Liao,X.P., Hu,Q.J., Liu,B.T., Fang,L.X., Deng,H.,
            Ma,J., Xiao,X., Zhu,H.Q. and Liu,Y.H.
  TITLE     Prevalence and plasmid characterization of the qnrD determinant in
            Enterobacteriaceae isolated from animals, retail meat products, and
            humans
  JOURNAL   Microb Drug Resist 19 (4), 331-335 (2013)
   PUBMED   23557071
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Zhang,S., Sun,J. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2012) South China Agricultural University, College
            of Veterinary Medicine, Wushan, Guangzhou, Guangdong 510642, China
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 01/03/2024 06:27:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JQ776505.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="CGF41"
                     /isolation_source="pork"
                     /db_xref="taxon:546"
                     /plasmid="pCGF41"
                     /geo_loc_name="China"
                     /collection_date="2010-07-13"
                     /collected_by="Jian Sun"
                     /PCR_primers="fwd_name: invdr/f, fwd_seq:
                     caggcgcttcagcttgtt, fwd_name: qnrd-f, fwd_seq:
                     cgagatcaatttacggggaata, rev_name: invdf/r, rev_seq:
                     tattccccgtaaattgatctcg, rev_name: qnrd-r, rev_seq:
                     aacaagctgaagcgcctg"
     gene            554..1198
                     /locus_tag="HUF28_RS00005"
                     /old_locus_tag="D726_p8001"
     CDS             554..1198
                     /locus_tag="HUF28_RS00005"
                     /old_locus_tag="D726_p8001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrD1"
                     /protein_id="WP_012634451.1"
                     /translation="MEKHFINEKFSRDQFTGNRVKNIAFSNCDFSGVDLTDTEFVDCSF
                     YDRNSLEGCDFNRAKLKNASFKSCDLSMSNFKNISALGLEISECLAQGADFRGANFMNM
                     ITTRSWFCSAYITKTNLSYANFSRVILEKCELWENRWNGTVITGAVFRGSDLSCGEFSS
                     FDWSLADFTGCDLTGGALGELDARRINLDGVKLDGEQALQLVESLGVIVHR"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrD1"
     gene            complement(1818..2204)
                     /locus_tag="HUF28_RS00010"
     CDS             complement(1818..2204)
                     /locus_tag="HUF28_RS00010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058671329.1"
                     /translation="MRISNVEWLKKRIGFIRKLGEKTTRQRQIIDLLDDEASLSETDRR
                     LLHVLATAEKNELQEQDAAQKKAVQARISGQKNRRERNHKLFLAAGLMIDAGLVDTKTG
                     ELNFDSEKLLTALKRVRCDLETSV"
                     /name="hypothetical protein"
     gene            2372..3307
                     /locus_tag="HUF28_RS00015"
     CDS             2372..3307
                     /locus_tag="HUF28_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_072206570.1"
                     /translation="MATYHLSVKFGGKGKALAHANYITREDKFSQRKDLEHTEHGNMPE
                     WAKDDPAHFWQAADAFERANGSTYREIEIALPRELNEAQRLELVRDFVKQETGGQHTYS
                     FAIHNPQASIDGGEQPHAHIMMSQRVDDGIKRSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKAQGIDRKPEQHLGFEAAGR
                     LDDSQRQDIKDSRTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQY
                     GREMAARVTKQGNVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     gene            3310..3840
                     /locus_tag="HUF28_RS00020"
     CDS             3310..3840
                     /locus_tag="HUF28_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     ARG             555..1195
                     /name="qnrD1"
                     /gene="qnrD1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrD1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          305..460
                     /name="Col3M"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.089"
                     /coverage="100.0"
                     /evalue="2.29e-71"
     Typing          2373..3304
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.678"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcactcggt tcgcggggct acagcgtttc tgtgtccgaa accgaacgat tacgtacagc
       61 atgtagacaa catgtccgaa agtaagcaaa accattaacg gacagccgga tcatttcgta
      121 catcccttac ggtcaggggt tcgcgctcaa tggaacgaaa tcacccgtta gcttaaaacg
      181 cttaacgtag gataatttcc tttttccaag cggacccgta ctcggttggt caaatcgctt
      241 cttgccacaa aacaagtttg tgtctttcag cgatttcgca tcgttttact tgtcagtttg
      301 aatgatgtta aaaacgcact cagaagctcg caaaaccgtt ctaaactttc agcgtagatt
      361 tgggtgtttt atagggtgat agtgtcttaa aatcgttttg aggggcttta gtggggatga
      421 acgggcggtt ttcgctaact aactcgcccg tttaacataa aagggattgt atgaatcaat
      481 tttagctaga gtttaaggtt gttcaaatta atgtacaatg attgcactgt ataaataacc
      541 aggtgtagca tgtatggaaa agcactttat caatgaaaag ttttcacgag atcaatttac
      601 ggggaataga gttaaaaata ttgccttttc aaattgtgat ttttcagggg ttgatttaac
      661 tgatactgaa tttgttgatt gtagttttta cgacaggaat agcttggaag ggtgtgattt
      721 taatagagcc aaactaaaaa acgctagctt taaaagctgc gatttatcaa tgagtaattt
      781 taaaaacatt agcgccttag gtcttgaaat tagtgagtgt ttagctcaag gagctgattt
      841 tcgaggggct aattttatga atatgataac tacaaggtca tggttttgta gtgcttatat
      901 aaccaagaca aatcttagtt acgctaattt ttctagagtc atattagaaa agtgcgaact
      961 gtgggaaaat cgctggaatg gcactgtgat aactggcgcc gtgtttcgtg gctccgatct
     1021 ttcttgtggg gagttttcat cgtttgattg gtctttggct gattttactg gttgtgattt
     1081 aacgggtggg gcgcttggcg agcttgatgc aaggcgaatt aatttagatg gagtgaagtt
     1141 ggatggagag caggcgcttc agcttgttga gagtttaggt gttattgttc accgataaaa
     1201 tctaggtaaa aaacgcctaa tgccccaatg tggtactaat caaaacggag cgaagcgaca
     1261 tcttcatctt gtctttgctt ttctccatga tcagttattg ggtcatggtc tcttagctcg
     1321 ttaatctggc taagtttggt taggttgggc ttctttcgac agtgcaatcc aagaaatccc
     1381 tagtccgttg attggtgctc ttatcaggta cgcactgtaa tctttcgata ccgcagcgtg
     1441 attcggaggc agagccggaa tttcgttttc cggtacgttt tactaccaca atttgcctgt
     1501 ttatcctttc ggagaccggg ctaagtccga gtcaatcaag tggtgcattt gagttaatgt
     1561 cgttgaatgg ttttaaggaa gtagtgacaa gctagcttcc cattgctaag attggattgt
     1621 ctaggtctga tcttagcaac gaatgcaccg aatcagatta gcagagccgg agtgatgtca
     1681 caatcatttc ggctctgcgc tttctgaact taagcaaaaa atcccctcgt atttcaatct
     1741 gcaacgctcg ccgcaagatg caaactggtt ttgcatcgag gaagcgtcat atcactgaat
     1801 ccttgcagaa tgcacggtca caccgatgtt tccagatcac agcgtacccg tttcagcgcg
     1861 gtcagtaatt tttcagagtc aaaattcaac tcacccgttt ttgtatcaac cagtcccgcg
     1921 tcaatcatca gtccggcagc caagaacagt ttgtgattac gttccctgcg gtttttctga
     1981 cctgaaatcc gtgcctgaac cgcctttttc tgcgcggcat cctgttcctg caactcgttt
     2041 ttttcagcgg ttgccagaac gtggagcagt cgcctgtcag tctcactgag cgaagcctca
     2101 tcatccagca ggtcgataat ctgccgttgc cgtgttgttt tctcccccag ttttctgatg
     2161 aagcctatgc gctttttaag ccattcgacg ttactgatcc gcatcgttta acttccccat
     2221 cccgatattt ccgatggtga aagtatataa cttgcggtca gaagtcgcat gacttatctt
     2281 tactcataat tcttcaaatt agagtaaagc gcacttatat gtttctccct gcgggcagaa
     2341 acacgtgcgt aaaaccaagg gcaaaaacat catggcaacc tatcatctca gcgtgaaatt
     2401 tggcggcaag ggtaaagccc ttgctcatgc caattacatc acgcgggaag ataagttcag
     2461 tcagcgaaaa gatctcgaac acaccgagca cggcaatatg ccggaatggg cgaaagatga
     2521 ccccgcccat ttctggcagg ctgcggatgc gttcgaacgt gccaacggtt caacgtaccg
     2581 ggaaatcgag atagcactac cccgtgaact gaacgaagcg cagcggctgg agcttgtcag
     2641 ggattttgtg aagcaggaaa cgggcggtca acatacgtat tcgtttgcga ttcacaatcc
     2701 gcaagccagc attgacggcg gcgaacagcc gcacgcccat atcatgatga gccagcgtgt
     2761 tgatgacggt attaagcgca gcccagagca gtatttcagg cgctataacg ccaaatttcc
     2821 ggcgcgtggc ggcgcgcgca aggactcagg gcataattta acccctacag aacaaaaaca
     2881 ggcactgaaa gccctgcggg agcgctggga gcgtacgcac aacgcgcata tgaaacgata
     2941 tggctttgag cagggtttta ttgatagccg gtcactgaaa gcacagggta tagaccggaa
     3001 accggaacag catctcggtt ttgaagcagc cggacgactg gacgacagcc agaggcagga
     3061 tattaaagac agccggacgt tttcagtcgt ggatgtgcct gaacaggcga agcatatcga
     3121 ccacagcgac cgtagctgga tgaatgccag cggtatcgag gtaaaccgga aggaactggt
     3181 cagatctctg acgcatgata tctcgcagca gtacgggcgg gagatggctg cacgggtcac
     3241 aaaacaggga aatgttgaaa tagagcgcgt gaaatccctg tcacgcggat tttccataag
     3301 caggtaacta tggcgagcac cgaagaaaaa cttctcacac tcctgctgtc ggcggagcat
     3361 ctgaaagagg ccgcaaaaga gcagcagaaa gccgtggcgg attcgctggc ggtggcaagg
     3421 gaaaccctga aaggctaccg ggaaatatca gacagtgtgg cacagcaggt acgggatgaa
     3481 gtccggcagg aagtgcagaa aatggatgtt gcggggctga ttggcgagcg tatcagcgct
     3541 gattttcagc gtctggaaaa atccgtcggg aacatgaaaa tcaatgcgga cgcgctggac
     3601 aaggaaattt ccattatccg cagcaatatc atcgcggagt acaacacgct gcggggttac
     3661 agctggaagt ggtttgcagg ggttgcggtt gcctttgtcg tactgatatt tgccacaagc
     3721 tggtttgtga aaacggaact acaggacaat tacaacgcgt ctgctgccgt ctatcagaaa
     3781 gtggcagacc ttgaaaagct gctgacaacg gaacagaaac cggaaaagaa aaagaactga
     3841 cgcgctgcgc ttgtccaacg cctcacgttc gcacaataaa ttgcgcaccc gttcaccgtt
     3901 ttcagccggg tttggggcgt cttttgagac ttccggcctg aattcaggcc ggaagttctc
     3961 gccagtcagt caggtgcatg gttatgcatg gggctgaaaa tcacggtatg cgattctgag
     4021 cggttttcgg tgagaagacg ggggttttta tggctcagga cgtaaaaccg ccctgagcgc
     4081 gtttcagcgg tgcgcgtaat gacgcgttat ggtaaatcgg tgccacaggc gtaagtatct
     4141 gctaaggatc tttttgttga ttctttttta agagcataat gagattatat aaattgattt
     4201 tggggtccgc ttggaaaagg gaaaataacc tacgctaaga ctacttttta accaatcggc
     4261 tattggat
//
