LOCUS       NC_021511               2687 bp    DNA     circular CON 02-JUL-2023
DEFINITION  Escherichia coli plasmid pCGS13, complete sequence.
ACCESSION   NC_021511
VERSION     NC_021511.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226287
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Zhang,S., Sun,J., Liao,X.P., Hu,Q.J., Liu,B.T., Fang,L.X., Deng,H.,
            Ma,J., Xiao,X., Zhu,H.Q. and Liu,Y.H.
  TITLE     Prevalence and plasmid characterization of the qnrD determinant in
            Enterobacteriaceae isolated from animals, retail meat products, and
            humans
  JOURNAL   Microb Drug Resist 19 (4), 331-335 (2013)
   PUBMED   23557071
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Zhang,S., Sun,J. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2012) South China Agricultural University, College
            of Veterinary Medicine, Wushan, Guangzhou, Guangdong 510642, China
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 07/02/2023 04:21:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JQ776506.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CGS13"
                     /isolation_source="pig liver"
                     /db_xref="taxon:562"
                     /plasmid="pCGS13"
                     /geo_loc_name="China"
                     /collection_date="2010-08-26"
                     /collected_by="Yang Wang"
                     /PCR_primers="fwd_name: invdr/f, fwd_seq:
                     caggcgcttcagcttgtt, fwd_name: qnrd-f, fwd_seq:
                     cgagatcaatttacggggaata, rev_name: invdf/r, rev_seq:
                     tattccccgtaaattgatctcg, rev_name: qnrd-r, rev_seq:
                     aacaagctgaagcgcctg"
     gene            38..682
                     /locus_tag="HUF32_RS00005"
                     /old_locus_tag="D616_p101001"
     CDS             38..682
                     /locus_tag="HUF32_RS00005"
                     /old_locus_tag="D616_p101001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrD1"
                     /protein_id="WP_012634451.1"
                     /translation="MEKHFINEKFSRDQFTGNRVKNIAFSNCDFSGVDLTDTEFVDCSF
                     YDRNSLEGCDFNRAKLKNASFKSCDLSMSNFKNISALGLEISECLAQGADFRGANFMNM
                     ITTRSWFCSAYITKTNLSYANFSRVILEKCELWENRWNGTVITGAVFRGSDLSCGEFSS
                     FDWSLADFTGCDLTGGALGELDARRINLDGVKLDGEQALQLVESLGVIVHR"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrD1"
     gene            1320..2036
                     /locus_tag="HUF32_RS00010"
     CDS             1320..2036
                     /locus_tag="HUF32_RS00010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_173479974.1"
                     /translation="MSQLFRLASPEDYEYIEELNLWELSFDKRPVQGVRCEDPVIGAEK
                     YNQGREKFKALASRHKKHWKPNGLTFSELIRWAIECGTPEQCQLVLALYHCTNDDDYQA
                     MGIQLSRSIDNANVSPIIFFTGKNQRLLGDISMLKQARKKKDALTLGLPIILCIILSII
                     LSLILSISSVIGRIIHRIRYRIIDRHKMTLCYFLPINPPVILPITLPVIAPNLGITGRI
                     TGEIMGRIIVLLTCTP"
                     /name="hypothetical protein"
     ARG             39..679
                     /name="qnrD1"
                     /gene="qnrD1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrD1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2476..2631
                     /name="Col3M"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.089"
                     /coverage="100.0"
                     /evalue="2.29e-71"
ORIGIN
        1 aatgattgca ctgtataaat aaccaggtgt agcatgtatg gaaaagcact ttatcaatga
       61 aaagttttca cgagatcaat ttacggggaa tagagttaaa aatattgcct tttcaaattg
      121 tgatttttca ggggttgatt taactgatac tgaatttgtt gattgtagtt tttacgacag
      181 gaatagcttg gaagggtgtg attttaatag agccaaacta aaaaacgcta gctttaaaag
      241 ctgcgattta tcaatgagta attttaaaaa cattagcgcc ttaggtcttg aaattagtga
      301 gtgtttagct caaggagctg attttcgagg ggctaatttt atgaatatga taactacaag
      361 gtcatggttt tgtagtgctt atataaccaa gacaaatctt agttacgcta atttttctag
      421 agtcatatta gaaaagtgcg aactgtggga aaatcgctgg aatggcactg tgataactgg
      481 cgccgtgttt cgtggctccg atctttcttg tggggagttt tcatcgtttg attggtcttt
      541 ggctgatttt actggttgtg atttaacggg tggggcgctt ggcgagcttg atgcaaggcg
      601 aattaattta gatggagtga agttggatgg agagcaggcg cttcagcttg ttgagagttt
      661 aggtgttatt gttcaccgat aaaatctagg taaaaaacgc ctaatgcccc aatgtggtac
      721 taatcaaaac ggagcgaagc gacatcttca tcttgtcttt gcttttctcc atgatcagtt
      781 attgggtcat ggtctcttag ctcgttaatc tggctaagtt tggttaggtt gggcttcttt
      841 cgacacttcg caatccaaga aatccctagt ccgttgattg gtgctcttat caggtacgca
      901 ctgtaatctt tcgataccgc agcgtgattc ggaggtagag ccggaatttc gttttccggt
      961 acgttttact accacaattt gcctgtttat cctttcggag accgggctaa gtccgagtca
     1021 atcaagtggt gcatttgggt taatgtcgtt ggctggtatt taatgagaac gtgacaagcg
     1081 cctctcacgt tgctaagatt ggattgcgac gtctaatctg agcaacgaat gcacaagatt
     1141 tagagtacca gagccctgtg atgctgtcac ggggctttgt tacatctgga gcttggtaaa
     1201 cctcataaat tcaattcgca acgctcgccg caaggcgtga acttgtttca cgtcgaggaa
     1261 gcggaatttc tcgaaagcct tatactgtct ccgttcacac atgatcggag ggtgtctcta
     1321 tgtctcaatt atttcgtttg gcatcgccag aagattacga atacatcgaa gaattaaatt
     1381 tatgggaatt gtcatttgat aagcgaccgg ttcaaggggt tcgttgtgaa gatccggtga
     1441 tcggggcaga aaaatataat caagggcgtg aaaaatttaa ggcgttagcg tcacggcata
     1501 aaaaacactg gaaaccgaac ggattgacct tttcggagtt gatccgctgg gcaatagaat
     1561 gcggaactcc agaacaatgt cagcttgttt tagcgttata ccattgcaca aacgatgatg
     1621 attatcaggc gatgggaatt cagttaagcc gaagtattga taatgcaaat gtcagtccga
     1681 tcatcttttt cacaggtaaa aatcaacggt tattaggtga tatttcgatg ctaaaacaag
     1741 ctcgaaagaa aaaagatgcg ctgacgttag gtttacctat tattttatgt attatcctat
     1801 ctattattct atctcttatt ttatctattt cgtctgtaat aggtagaata atacatagaa
     1861 taagatacag aataatagat agacacaaga tgacgttgtg ttatttctta cccattaatc
     1921 cacctgttat tctacctatt actttacccg ttattgcacc caatttaggt ataacgggca
     1981 gaataacggg tgaaataatg ggtagaatca tagttttact aacttgcacc ccatgatact
     2041 ttcagtatca atgccactca ttttctggac gaaaatgaat gacataaggc tccgccctct
     2101 ggctattttc ttcggctgga cgcctgcgaa aaaaaccatt cacccaagcc cgtcaaactc
     2161 actgcgttcg tctgaccacc cccttttttc gcccctgtat ttggtcgctc aaaaatgtgg
     2221 tggtcagacg cttcgcttga cggggggctt tgcccccaaa acgaaacacg ttcactgggg
     2281 ttcacttgcc atcgctgggg cgtgtcattt cgtttcggct ccccctgttt ttccctttct
     2341 tcgctggacg ctcatcagga taaaaacaaa aagcctaccg ccctcgtttt actcggttgg
     2401 tcaaatcgct tcttgccaca aaacaagttt gtgactttca gcgatttcgc atcgttttac
     2461 ttgtcagttt gcacgatgtt aaaaacgcac tcagaagccc gcaaaaccgt tctaaacttt
     2521 cagcgtagat ttgggtgttt tatagggtga tcgtgtctta aaatcgtttc gaggggcttt
     2581 aatggggatg aacgggaggt ttttgctaac taactcgccc gtttaacata aaagggattg
     2641 tatgaatcaa ttttagctag agtttaaggt tgttcaaatt aatgtac
//
