LOCUS       NC_021489               8970 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Acinetobacter baumannii plasmid pAB-NCGM253, complete sequence.
ACCESSION   NC_021489
VERSION     NC_021489.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1
  AUTHORS   Tada,T., Miyoshi-Akiyama,T., Shimada,K., Shimojima,M. and Kirikae,T.
  TITLE     Dissemination of 16S rRNA methylase ArmA-producing acinetobacter
            baumannii and emergence of OXA-72 carbapenemase coproducers in Japan
  JOURNAL   Antimicrob Agents Chemother 58 (5), 2916-2920 (2014)
   PUBMED   24550340
REFERENCE   2  (bases 1 to 8970)
  AUTHORS   Tada,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2013) Contact:Tatsuya Tada National Center for
            Global Health and Medicine, Department of Infectious Diseases;
            1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009941255.2-RS_2024_05_27
            Annotation Date              :: 05/27/2024 02:32:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 10
            CDSs (total)                 :: 10
            Genes (coding)               :: 10
            CDSs (with protein)          :: 10
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AB823544.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="NCGM 253"
                     /db_xref="taxon:470"
                     /plasmid="pAB-NCGM253"
     gene            199..570
                     /locus_tag="HXD21_RS00005"
                     /old_locus_tag="D721_p5001"
     CDS             199..570
                     /locus_tag="HXD21_RS00005"
                     /old_locus_tag="D721_p5001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(1066..1527)
                     /locus_tag="HXD21_RS00010"
                     /old_locus_tag="D721_p5002"
     CDS             complement(1066..1527)
                     /locus_tag="HXD21_RS00010"
                     /old_locus_tag="D721_p5002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(1832..4243)
                     /gene="znuD2"
                     /locus_tag="HXD21_RS00015"
                     /old_locus_tag="D721_p5003"
     CDS             complement(1832..4243)
                     /gene="znuD2"
                     /locus_tag="HXD21_RS00015"
                     /old_locus_tag="D721_p5003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            complement(4371..4685)
                     /locus_tag="HXD21_RS00020"
                     /old_locus_tag="D721_p5004"
     CDS             complement(4371..4685)
                     /locus_tag="HXD21_RS00020"
                     /old_locus_tag="D721_p5004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(4672..4959)
                     /locus_tag="HXD21_RS00025"
                     /old_locus_tag="D721_p5005"
     CDS             complement(4672..4959)
                     /locus_tag="HXD21_RS00025"
                     /old_locus_tag="D721_p5005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            5268..6095
                     /locus_tag="HXD21_RS00030"
                     /old_locus_tag="D721_p5006"
     CDS             5268..6095
                     /locus_tag="HXD21_RS00030"
                     /old_locus_tag="D721_p5006"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            complement(6278..6421)
                     /locus_tag="HXD21_RS00035"
     CDS             complement(6278..6421)
                     /locus_tag="HXD21_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(6441..7016)
                     /locus_tag="HXD21_RS00040"
                     /old_locus_tag="D721_p5007"
     CDS             complement(6441..7016)
                     /locus_tag="HXD21_RS00040"
                     /old_locus_tag="D721_p5007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(7009..7959)
                     /gene="repM"
                     /locus_tag="HXD21_RS00045"
                     /old_locus_tag="D721_p5008"
     CDS             complement(7009..7959)
                     /gene="repM"
                     /locus_tag="HXD21_RS00045"
                     /old_locus_tag="D721_p5008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            8643..8897
                     /locus_tag="HXD21_RS00050"
                     /old_locus_tag="D721_p5010"
     CDS             8643..8897
                     /locus_tag="HXD21_RS00050"
                     /old_locus_tag="D721_p5010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     ARG             5269..6092
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(7010..7959)
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgataaatt taagaaaaaa tatgtactca tgattgtaca taaaaaaagc attatattta
       61 aataagtttt tgttttagaa taaattaatt ctgtacaaat aagtttgtac gtaatctaat
      121 tattattttt tatttaatta gttgaattat atatatttaa ttttgtacaa aatctaattt
      181 taataagagg tgtaatttat gggcgaaaaa gtcaaaactt atgcagtcag gttagatcca
      241 caagtagcag aattctacga tcaattggct aattcagaag gaatcagaac cagtaaatta
      301 ttcagtaaaa ttttgaccaa tgattttcaa tctattgctg ttaaacagca agccgatcgt
      361 attgaggatc tggtgaatcg attagaaaag cgaatagatg attttgagga agataacaat
      421 ttttctgaaa aatactatga agacttttct ggactatatt tcatgatgtt gtggctctta
      481 atgaaaaatg gcgcttctaa agatgaggtt agaatgatac aagctaaagg ttattcatat
      541 gcagatgata actataagag ggtaaagtag tgtcagataa tattattcag ctttcaaagc
      601 catgtacgct ttgtgaaaat agacaaaatg tacaactttt tgcaggattg atgctgtgtg
      661 gaagttgtca ggaaaatatc aggcttacga atccaagtat gttctcggcc aatgatgaga
      721 ttgaacaaaa agcccagaat taacctgggc ttcgtataag agattttatg ttaaatgccg
      781 aactgaatta aaaatagaaa tgtagttgag ctgatgcttg ttcatttctt tatttttagt
      841 aattgaggtg aacatactgt cagtacatgg tagttataag taacgtgtaa ctttgtttgc
      901 acaaagattt gagttatgcc aagtgatgga ttgatttgta cttaaaattt taagcttatc
      961 cacttgcttt gcaagccact acactttatt tacaatccta tgttgtggaa gtaagggatc
     1021 ttgtactcgc ccagtatgtt caacttgatt atatctaaag gactattagg cttcaatcaa
     1081 atcaattttc caattagtcg actgaattaa cacgtttaaa tcacgtaatt taagactaat
     1141 atcatcagct tgtttttgta atgatgaaac tggaataact ttatgccact ttatttcccg
     1201 cgtactataa cgatcagatt cacgagatgt attacttatt gctgcagtta gggttttgtg
     1261 ctgttctaca aattcatcac gcaaagtcaa taaagcaagt agagaacggc catcatttaa
     1321 ttttgttaaa gcattagttt catgaattgc ataacttaat ttttgaaatt cttgaattaa
     1381 agaaaatact tgttttagta gttcatttgg atcttcagat ggttgatcac catcttgtac
     1441 caatacattt gcattgatac gttgttttag tgaaataatt tttttttgtt gatcacttcg
     1501 aagtaagaga gcttctgcta atttcatttt tcacctatta accaattaga attaagtttt
     1561 aaacagctta tatcctcatt ctttaaagat caaaaaaatt gctttttatc tagctaaaag
     1621 gtgtctacaa aatcggtggc tattcagtgc tgaaaaggct gccgacacat aaagtgaccc
     1681 agattgaaga gttactgcca cactgctgga aacctaaatc gaattaaaaa ataggtatgg
     1741 gattcagcgg acgcttacct taattattaa tctaatcatt ttataagaaa aatcttctaa
     1801 gaaattttct tagaagatta cagtatctta tctagaaatt aagatttaag cctaaactaa
     1861 aatttcttcc aatttgggga atatatggca aaaatgtttc atgggcatag actttctgat
     1921 caagtaaatt attcgcttta aagaataaat catattctgt gtgagatagc ttatttttat
     1981 atgctaaagt catattaagc atattataac ctgatgtaac ctgctcaaat ttagaaatat
     2041 tatcttgttt aaagactcga taatattcta tttcaccttt aagcgattca tcaaaatcag
     2101 cttttagtcg tgtacccaaa cgagcaggag gtaatctagg agtgtaacga tccttttggg
     2161 gagctaaagt cggttctcta ttccagatat cgtatgcaat tacagcatca ggtaaatttg
     2221 ttaaacggcc tttcacataa tctccaaaaa gagagccgtg ataaacagaa ttaaattgat
     2281 accctatgtt gccctctaat ccataaaaat tagcagcaga ctggctataa tgattaatac
     2341 gtagtgtatg ctgatctcta acttttgtag ttcctaaaac ttcattgagt gtctgtaaat
     2401 atatatagtt atcaaaatca taatagtaag tactgatttg ataatcaagt aaatcatcct
     2461 tataggccag actgatttct aaattattgg atttctcttt tcttagaaag cggttaccta
     2521 tttcaaaaga attagtagcg agatgcatac catgagtata catttcttgt gcattaggta
     2581 atctttcttg atgtgtacct gttaatgata agatataatt tggagcaaat ctccagtgga
     2641 ctgtacctgc ataagaaaaa gctgtttctt tgttaggttg aacagcttct aaaatagatt
     2701 ttaaattttg agccctgatt ttattatttt tttctacata aggactgtta tatttattag
     2761 gccaaggttt cattgaatct ttaatttttt ctatatcata atccatacta actttctgct
     2821 tttctatacg agcacctagt tcgaaagtaa agtcgttcca gttatatttt tctaggccaa
     2881 atacactaaa gtttttagtg acatttcgat ttaatagctg ctgattatct agataggtgg
     2941 tatggccttc ttgagaatgg actggtgata aagcactatt atcctgttcc aaatattgta
     3001 gccccaagat accggttaat tcccctaatg gctgatgtga taactctatg cggcctactt
     3061 ttcctgtatt tttaaaaaag ttagtaatct tatctccctc gagttcattg tgaaaataat
     3121 ctatataact caatgaggta cgaattttgt caataccggt aaatggttga gtgaattctc
     3181 cacgcacatc ataacgtcga gtatttaaat caatataagg agcattatga gagaaattag
     3241 tttcatgaat atgattatgc tgatgacaat caggacgtgg atttatgtaa ttaatatctt
     3301 gctcatccat caattgagga taatataaaa gataattttt taatccacta attcgtgaat
     3361 cgataccgat tgcagaggca ccacaacctt catataaatg gctatgggct ggtaaaccat
     3421 attcatcatg acgatgtgta taagaaactc ctaaataagt attatcgtct atccaggata
     3481 atccaagcgt acctactctc gactctgccc agctttctgg taaatatttt aaatttttat
     3541 aaatactgtt ttggttttca agatcttcta aagaattata agagctagac tggtaatgag
     3601 gagttttata gttaccagca ttacgatata acccttctgc ccgtaaagct acatgtgggc
     3661 ttaagccaac tgttacacct gctgtggtca acttctcatt actgccagta ttaaaacgta
     3721 atgtagtatt accctctaat ccgtcttgag gcatttgggt tggaatttta tagtcaatga
     3781 cattgactac ccctgccgca ctatttgaac tatacaaaag ggtacttgca ccacgtataa
     3841 tttcaataga cttagctaaa cttggttcaa ctgtgacagc gtgatctggg gacatattag
     3901 acatatccaa cacgtcagca ttattctgta aaactttaat acgttttcct tcctgcccac
     3961 gaataatagg agtagatgct ccacctccat attgattaga atgaattcct aattccgtac
     4021 ccaaggcatc ccctagagtt gtcgaacgct gtttaagttt actagtatca ataaactgat
     4081 tactaacatt tagacttttg cctaacagct cagaccctaa ctcatcagat tgtgcagtta
     4141 atactattgt atttaaggta gtaacaggcc cttcagctgc caataaaatt ttcgaggccc
     4201 ctaaagtaat taaagctaaa aatagtttac ttttatttag cataaaacaa ccaaatttaa
     4261 tgttatatta taatataaca cttaaaaatt tataaaatct actatttaaa tttttgaatg
     4321 gtaatccccc ctaaaaatga agtcaaaaat gggagcctaa gctcccattc ttattgatga
     4381 ttttttaatt cttttagcat agcatctcgt aaaatttcat tcatacgtgt ttgataacct
     4441 ttgccttgtg ctttgaacca agcaagtaca tcagcatcta accgaattga agtttgttgc
     4501 tttactgggc ggtaaaattg attttggcgt acagcattgc tccagtctgt aatttcagga
     4561 atatctgata gatctagctg atcatcagga accgtgcctt tagcaagcaa gcgttgaatt
     4621 tcagcatctt gcttctcacc aaatttctca ttcagctctt tgtgtgagta tctaaccatg
     4681 ctcatatttg tttcgctccg ctttagtgac ttgccttgca ctaatgattc ggatgatttc
     4741 acagtcatct tcatcaaaaa tggtgtgagc caccaataac attagtacgc ctttaactcg
     4801 tccaatggtt tgccaacgtt cttcaccatt ggtatgtctg tcttggattg agatccgtaa
     4861 tggatcttca aaaacaaggc ttgcagtttc gaaagagata tcatgctttt tctgattctt
     4921 tcgattctta gcctcatccc attcaaaata ctgttccata aaaaacattc aaatttgtat
     4981 atacaataat gtatcacaaa attgtatcac tcaaccagat tcgtgtaatt tttagtcaaa
     5041 attattggca agaagtatca aaactgctca taaaaaagcc gacttgtttc aaagtcggct
     5101 ttttgggcat tttgggattt ttctattttt attaagtagt tgatatttat attaatatac
     5161 aagtgtactt cgcataacgt ccattatgtt aaattaaaag agttttaact tttttaataa
     5221 aaatattata ctctaagccc caaaatttcc cctaacatga atttgtaatg aaaaaattta
     5281 tacttcctat attcagcatt tctattctag tttctctcag tgcatgttca tctattaaaa
     5341 ctaaatctga agataatttt catatttctt ctcagcaaca tgaaaaagct attaaaagct
     5401 attttgatga agctcaaaca cagggtgtaa ttattattaa agagggtaaa aatcttagca
     5461 cctatggtaa tgctcttgca cgagcaaata aagaatatgt ccctgcatca acatttaaga
     5521 tgctaaatgc tttaatcggg ctagaaaatc ataaagcaac aacaaatgag attttcaaat
     5581 gggatggtaa aaaaagaact tatcctatgt gggagaaaga tatgacttta ggtgaggcaa
     5641 tggcattgtc agcagttcca gtatatcaag agcttgcaag acggactggc ctagagctaa
     5701 tgcagaaaga agtaaagcgg gttaattttg gaaatacaaa tattggaaca caggtcgata
     5761 atttttggtt agttggcccc cttaaaatta caccagtaca agaagttaat tttgccgatg
     5821 accttgcaca taaccgatta ccttttaaat tagaaactca agaagaagtt aaaaaaatgc
     5881 ttctaattaa agaagtaaat ggtagtaaga tttatgcaaa aagtggatgg ggaatggatg
     5941 ttactccaca ggtaggttgg ttgactggtt gggtggagca agctaatgga aaaaaaatcc
     6001 ccttttcgct caacttagaa atgaaagaag gaatgtctgg ttctattcgt aatgaaatta
     6061 cttataagtc gctagaaaat cttggaatca tttaataatt tctaaaatta acataatacg
     6121 ccttatacga aatgctgtat aattatcctt ataattcaac gctgtagttt ctagtttata
     6181 gcccgagcat ccctgttcgg gcttttttta tgacttttca cgttccacgc tattatttca
     6241 tcaatgttcc acacttttga ttagctcaat tgaataatca ggatgcctgg tctttttctt
     6301 tttggcattc atccagctct ttcttcagcg cctttttatc ctggtacgat gcaatcagca
     6361 tgatcgggat cagcagcgga gctacaataa ttcccagggc aatgtctttc agtttcggca
     6421 tgatctttca cccttattgc tcagtcatta tcaaagacgg cattcagcac acggccaaat
     6481 aaaccacgtt ttttgtgttg tggcactgcc acactttgca acgtggcatg ctgtagcgtt
     6541 tgggtgtggt atgccgtagt ttcttgctgt ggcactgcta cattttctgc aatatttaat
     6601 tcattattct ttttgttatc agtgtcttgt tgtgttgtcg catgctgtgg catcttggtg
     6661 tcgcatgccg tagcatcttg gtgtggtgct gctgtggcag gtgcaacact tttcagctca
     6721 atgagtgttt gcatacggtc aatttgttgc attaaccgtt cttcacgttc ttggtatttt
     6781 ttgacttcat tttctaaatg ggcaataagt ttttttgtag attcatcacc ttcttgctta
     6841 gatgattcaa ttttttgaga tgtttgctca ggttctccaa agcatcgtat gacctctgct
     6901 agatcgaatt gtccatcact ggagcgtgat aaacgtccat tacgtacagc tctgtaaatg
     6961 gttgatctat ccacatgaaa ttttttagat acttcactta gtgataactt agccatttac
     7021 ggtgtccttt ttatttgatg gcatgtatcc cacttttttt aggtatgggt atagctcttt
     7081 gaattgagcg ggatcttgta gcatctcagc aatacgtaca gcaaactgtt ggtagctttc
     7141 tgttccttgt gaatagcgac ccatttcagg gagttctgaa agtttatttg caaacatatg
     7201 ccgttgagca tcggtcatct ttgaaaaaag ctccaaagtg ttcggatctc tttctattga
     7261 tgggctatcc ttcttcttct gtttaaaggt aaaagaaaat cctgaaatag aacgtcctct
     7321 tttatgctgt tcatatttta cagttatatc cgtatgctca tttatttgtt taattgaaag
     7381 ctctaaaaca cgctctttta aatgagccat ccgtaaatat tcattatcaa gaacgcctaa
     7441 cttctttcta aattcttgta gttctatggt tggggtttta ccagtgcttc gccactgaat
     7501 tagaagctca tataagcgaa tggcataagc gctactaaga ctactaactt gctgtaattc
     7561 atatttagta aattgttctt ccagtcgggt tatgaaggga acaacagcag gtgcaaatat
     7621 taagtctaca gttgcttcat tgtcgttata agcaatttga gatacccaac gactcataac
     7681 atttcggata ttcccattag cttttttctc ttgatagcta aattgacgcg cgaataaatc
     7741 cttacaagca tcttttaagg cttgataagc tgtattacgg tgtacaccaa attgattgat
     7801 ataactatct gcatgaactt ctaatggatc atttgcatta atccctttcc cactttccct
     7861 tgcttcaaca atagccaata aaattaaacg ttgttctact aagtctaagt tatagcttgc
     7921 gttgattaag gcattgtcct ttacaactaa atctctcata gccactattt tttattgtgt
     7981 ataagtacac gttaatatta tttgtgtact aatgcaaggt aaacgtggac atattgcaag
     8041 gtaaacgtgg acatattgca aggtaaacgt ggacatattg caaggtaaac gtggacatat
     8101 aagagctaaa accctttatt cataaggctt tcaagccatt taaaagtatt taaaaacttt
     8161 aaaataatta aaagcccagg gaatgaaaaa ttgcccttgg ttttcgcttc gctcaaactc
     8221 tattgaactt cgctttcgct cagttcgttg gggcaatttt ttggttaata ctggcgtgac
     8281 tttaaaaaaa agcaaaagaa acttggaatt cgcttcgctc ataaaacttt tttactcgct
     8341 acgctcgatc caggagcaaa tttctgcacg aaacttacgc tttcttaggc taaagaggac
     8401 aaaaaaatgt ttttcagagg gtctaggcgc aaattttgat agttcgctcg tagacactcg
     8461 ctctatgcat ctatctaaag tcaaaatacc tctttatttc gctctcattt gcgttttttg
     8521 agctgttttg tgtttttcac tatgagatct atgttttcta ggtaaaggct cttaaatcgc
     8581 atttgaacgc gttctatgaa gcttttcata attaaagttt tgggttgaaa tttttaacct
     8641 ggatgaatgg acaaaaaatt gattttttac gtttttctaa acaaggaaat aaatactttt
     8701 ttccatgcaa aaccaagcac cggccctacg tcactttagc caattttcca atccaagatc
     8761 cggcacatgc cgggattttt ttgtcacaca ccacgcatct aacgatgaca cccctcaaag
     8821 ccttacagga taaggatttc agcgattttt tagggtctca gaaacgtgta cagaaaaatg
     8881 gtcttttttt agaataagtg attgaattat ctatttttta tgctgtgctc atggttgtac
     8941 atcaaaacaa cacttttttt agataagata
//
