LOCUS       NC_021293               4938 bp    DNA     circular CON 18-FEB-2024
DEFINITION  Cronobacter sakazakii plasmid pCSA2, complete sequence.
ACCESSION   NC_021293
VERSION     NC_021293.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225906
KEYWORDS    RefSeq.
SOURCE      Cronobacter sakazakii
  ORGANISM  Cronobacter sakazakii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Cronobacter.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Choi,Y., Kim,S., Hwang,H., Kim,K.P., Kang,D.H. and Ryu,S.
  TITLE     Plasmid-encoded MCP is involved in virulence, motility, and biofilm
            formation of Cronobacter sakazakii ATCC 29544
  JOURNAL   Infect Immun 83 (1), 197-204 (2015)
   PUBMED   25332122
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Kim,S., Choi,Y., Hwang,H. and Ryu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-DEC-2014) Department of Agricultural Biotechnology,
            Department of Food and Animal Biotechnology, Gwanak-ro, Gwanak-gu,
            Seoul 151-921, Korea
  REMARK    Nucleotide and amino acid sequences updated by submitter
REFERENCE   3  (bases 1 to 4938)
  AUTHORS   Choi,Y., Hwang,H. and Ryu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2013) Department of Agricultural Biotechnology,
            Department of Food and Animal Biotechnology, Gwanak-ro, Gwanak-gu,
            Seoul 151-921, Korea
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: SeqMan v. 8.1.3 (4) Intel
            Sequencing Technology             :: ABI 3730xl DNA analyzer
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/18/2024 02:33:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6
            CDSs (total)                      :: 6
            Genes (coding)                    :: 5
            CDSs (with protein)               :: 5
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KC663407.2.
            On Dec 10, 2020 this sequence version replaced NC_021293.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Cronobacter sakazakii"
                     /mol_type="genomic DNA"
                     /strain="ATCC 29544"
                     /culture_collection="ATCC:29544"
                     /type_material="type strain of Cronobacter sakazakii"
                     /db_xref="taxon:28141"
                     /plasmid="pCSA2"
     gene            175..402
                     /locus_tag="HTB25_RS00005"
     CDS             175..402
                     /locus_tag="HTB25_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039697198.1"
                     /translation="MPVNACGAALVLPRLIDASFSAGAGGFAVNMNIFLLFSPLKMAGF
                     LRVKSGAGHHAGMAEERAFSRPVAVIYNAT"
                     /name="hypothetical protein"
     gene            474..1760
                     /locus_tag="HTB25_RS00010"
                     /old_locus_tag="PCSA2_001"
     CDS             474..1760
                     /locus_tag="HTB25_RS00010"
                     /old_locus_tag="PCSA2_001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437803.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_306170312.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            2769..3092
                     /locus_tag="HTB25_RS00015"
                     /old_locus_tag="PCSA2_003"
     CDS             2769..3092
                     /locus_tag="HTB25_RS00015"
                     /old_locus_tag="PCSA2_003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007899679.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTAKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGVGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3301..>3735
                     /locus_tag="HTB25_RS00035"
                     /pseudo=""
     CDS             3301..>3735
                     /locus_tag="HTB25_RS00035"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3770..4261
                     /locus_tag="HTB25_RS00025"
                     /old_locus_tag="PCSA2_005"
     CDS             3770..4261
                     /locus_tag="HTB25_RS00025"
                     /old_locus_tag="PCSA2_005"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_016355729.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGRQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESNDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            4268..4483
                     /locus_tag="HTB25_RS00030"
                     /old_locus_tag="PCSA2_006"
     CDS             4268..4483
                     /locus_tag="HTB25_RS00030"
                     /old_locus_tag="PCSA2_006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2482..2654
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.14e-86"
     Typing          complement(2011..2557)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.545"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          2770..3086
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="3.03e-59"
     Typing          3083..4584
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.692"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggggtgttat gttaaaagcc gaggtctggc ggatacggaa aaaattatgc cggccgggct
       61 tatgcccgcg gcaatccggc cgatacttaa attatccttc acaaaggatg tgctgcgatc
      121 caggcagttg agtgttttcc ggctcccgga tgtgagccat ttttttgcct tctgatgcct
      181 gttaatgcat gcggagctgc gctggttctt ccaagattga tagacgccag cttctctgct
      241 ggagccggcg gatttgccgt aaatatgaac attttcttat tgttcagccc gctaaagatg
      301 gcgggttttt tacgcgtgaa atccggagcc ggacatcatg ccggcatggc tgaagagcgg
      361 gctttttccc gccctgtcgc agtaatatat aacgctacat aaggagtagc cgataaccat
      421 ggcagtactt acattatccg ggcaatctga ttcatatgtt ttcacttaaa aatatgttct
      481 ccctgaacct ccagcgatca gccatttcgt atgacgcaat ttgtcagaat aagcctgtca
      541 ttgagttcag tccggagggg gtgataaata aagccagccc gctttttctc tccactatgg
      601 ggtacagggc tgatgaaata atcggtcacc accatcgtat attctgcccg ccctcgctgg
      661 taagttcacc ggaatacagc cagttctggc agcgccttgc caggggagaa agctttagtg
      721 gcaagtacct gcggctggca aagggaaatc gttccgtatg gcttgaagcc agttatatcc
      781 ccgtttctga caggcgcggc cgtgttatca gagtcattaa aattgctgcc gatatttctg
      841 agcgcgtgca ttctgctctt gagcaggaag cagtcgtaaa tgccataaac cgttctatgg
      901 ccatcatcac cttcaatcct gaagggatcg tgcttgaagc aaatgaaaat ttcgtcaatg
      961 ccactggcta taagcgggat gaaatcatag gtaagcatca tcgtctgttc tgtgccgaga
     1021 cgctttacaa aagtgatgaa taccgacatt tctgggagag tctgaatcaa ggtgagtttt
     1081 tttctggtct gtttccacgc cttaaccgtc agggagaccc tctgtggttc cgtgcaactt
     1141 ataaccctgt ctttaacagt gatgggcagc tttataaaat tgtgaaattc gctacagatg
     1201 ttacggctga tgtcctgcga aaccagagag agcaagaggc tgctgtgcat gcatgggata
     1261 tggccgtgca gacccgagaa agtgcgcaga acggtgccaa tgttattgaa aatagcatcc
     1321 ttatgattga cagaattgcg caggggatgg gggctgtctc caccgatatc tcacggctca
     1381 acaatcagtc tgaaagtatt gacgatatgg tggaaaccat ccggaagttt gccatgcaga
     1441 caagacttat tgcactgaat gccgcgattg aagcagcaag ggctggcgca tccggaagaa
     1501 gttttgcggt tgttgccgca gaagtgcgta atcttgcggc gagcgtcagt agcgcgactg
     1561 aagaaattga gcaagtcgtg gccagcaata gtcaactggc caaggatgtt ctctgtggca
     1621 ttgaaaacag cctgatgaac actcgggaag gggttacact catgcgcgaa gcgggtgaag
     1681 ttatgaccag cattcagagg aatgctgcag gggttgagac tgcggttaag gatgtcgcca
     1741 tttcagttaa agccggatag gccatggtgc atatccccat atctgtcgaa catcacagat
     1801 gatcttcttg agatcctttc cgttgcgtcg taatctcttg ctctgaaaac gaaaaaacca
     1861 cccggcaagg tggctttttc gaaggttcgc aagagttgac gctcttttga accgcggtaa
     1921 ctggcttgga ggagcacagt caccaaaact gtcctttcag tgtagcctta tcgcacccga
     1981 aacttcaaga ctctcctcct ctaaacctgt taccagtggc tgctgccagt ggcgctttgt
     2041 cgtgtttttc cggattggac tcaagttgat agttaccgga taaggcgcag cggtcggact
     2101 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgccttcccg gaactgagtg
     2161 tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag
     2221 gcaggaacag gagagcgcac gagggagcca ccagggggaa acgcctggta tctttatagt
     2281 cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg
     2341 cggagcctat ggaaaaacgg cttcgccgcg gccttatcgc ttccctgtta agcttctttc
     2401 tggcatctcc caggaaattt ccgccccgcc tgtaagccgg taccgctcgc cgcagccgaa
     2461 cgaccgagcg cagcgagtca gtgagcgagg aagcggaata tatcctgtat cgcatattct
     2521 gctgacgctc cgctgctgca tttttctcct gccacatgaa gcacttcact gatattcgca
     2581 tccgtgccaa catagtaagc cagtatacac tccgctagcg ctacgtgact ggttcagggc
     2641 tgcgccccga aacccgctaa cagccactga cgcgcctgcg gcttgtccaa ccccgcgccg
     2701 accgggaaag gttttcccgc ccgtcccggg gttatcagga gactgttttg tcagagaaac
     2761 ggaacaagat gctcaccatg tgggtgacgg aggatgagca caggcggcta cttgagcgct
     2821 gcgacggcag gcagctggcg gcgtggatgc ggcagacctg cctggacgaa aagccggctc
     2881 gtaccgcaaa acttccttcg atctcgccgg cgctgctgcg ccagcttgcc ggtatgggca
     2941 acaacctgaa ccagatagcc cgccgtgtta acgccggcgt cggtaccggt catgaccggg
     3001 tgcagattgt ggcggcgctg atggccatcg atgccgggct tgagcggctg cggcacgccg
     3061 ttcttgagaa aggaacggac gatgatcgtt aaatttcatc cacgcggtcg cggcggtggc
     3121 gccgggccgg tggactatct gctgggtaaa gaccggcagc gtgaaggcgc cagcgtcctg
     3181 caggggaagc cggaagaagt ccgggagctg atcgacgcgt cgccttacgt gaagaaatac
     3241 acctccggcg tcctgtcgtt tgcggaggcc gatctgcctc ccggccagcg ggaaaaactg
     3301 atggccagct tcgagcgggt tctgatgccc ggacttgata aagatcagta cagcattctg
     3361 tgggtggagc acgaggacaa ggggcgtctg gagctgaact ttctgatccc caataccgag
     3421 ctgctgaccg gcaggcggct ccagccgtac tacgaccgcg ccgaccggcc gcgtatcgat
     3481 gcctggcaga ccatcgtgaa cggcagactg gggctgcacg atccgaacgc gccggaaaac
     3541 cgccgggcgc tggtcacgcc ggccggactg ccgaaaacga agcaggaagc cgcagaagcc
     3601 atcacccgtg gactgctgag cttagcctcg tccggggagc tgaaaagccg tcaggacgtc
     3661 actgaggctc ttgaaggtgc aggttttgag gtggtgcgta caacgaaaag cagtatcagc
     3721 attgccgacc ctgacggggg gcgaaacatc cgactgaagg gagccatcta tgagcagtct
     3781 tttaacgctg gcgaaggact tagagcagca atcgaaagcg cagcagaaga gtaccggcga
     3841 gatgctgaaa gccgcattca gcgagcacga gcagtctgtc agagcggaac tgaacgcaag
     3901 cgcgaggaga atcagcgacg ccatcagcgc ccacgagcag agtatgagcg aggcgatgga
     3961 gaaaaaccgg cggagcgtgt tgctgacggc cgggaggaca tggctcacca tcctgatggt
     4021 gtcagcgctg ctgatcgcca cgagcgggag cattctgtgg tggcagggac ggcagataac
     4081 cgacaattac acacatcttc gtcagcagga agataccctg gcgaaaatga ccgcccggac
     4141 gtggggagta cgctatcagg agagcaacga cggccggagg ttccttatcc tgccgccggg
     4201 gatgcaagcc gaagccatcc cctacgacgg gacgacctgg atccgcctga aacaggagta
     4261 gcggaaaatg acagagctgg aaaagcagtt gctgagcgca ttagagcagc tacagcagga
     4321 ctactcgcaa aggctggacg aatgggagag cgccttcgcg gaatggcgga gcatgtgtgg
     4381 acttatgcaa cgggagaacg cggtgctgaa cgagcgcgtg tcggacttga gcacgcaggt
     4441 gctgagttta agcgagcagc tgcgccgctt gtccgggaac tgaatgccat cgaggagaag
     4501 gctgcactgg agtatcagcg tcagaacgag ctggagctga cgctccgaca gaagcagaag
     4561 agtttcgatg gtccgaagat gaggtgacag cccgcacgaa gaagggctca tcgcgttcct
     4621 gctaaatagg ctgactaccg gaatggtagt gacttatccc ggaggaagtg atgaaaattc
     4681 tcaacgttat tgaagccctg ctgaactgtt tttacctggt gatggagctg tgtgagttgc
     4741 cggagaggca tttgccggtt tttaaggcgt tcgcgctgat tttagtgtga ctttacgctg
     4801 catgcatctg gctgcatgac tatgcatcag tgtaatatcc agcggctgcg tttttggtgt
     4861 tttttcgtca tgtctgtcgc cactttctcg cgtctggcac cggaaacgca gccaaccccg
     4921 tttctgcggt gcgggcaa
//
