LOCUS       NC_020391              11331 bp    DNA     circular CON 07-APR-2024
DEFINITION  Bacillus thuringiensis serovar thuringiensis str. IS5056 plasmid
            pIS56-11, complete sequence.
ACCESSION   NC_020391
VERSION     NC_020391.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02604143
            Assembly: GCF_000341665.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis serovar thuringiensis str. IS5056
  ORGANISM  Bacillus thuringiensis serovar thuringiensis str. IS5056
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 11331)
  AUTHORS   Murawska,E., Fiedoruk,K., Bideshi,D.K. and Swiecicka,I.
  TITLE     Complete genome sequence of Bacillus thuringiensis subsp.
            thuringiensis strain IS5056, an isolate highly toxic to Trichoplusia
            ni
  JOURNAL   Genome Announc 1 (2), e0010813 (2013)
   PUBMED   23516221
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11331)
  AUTHORS   Murawska,E., Fiedoruk,K., Bideshi,D.K. and Swiecicka,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2013) Department of Microbiology, University of
            Bialystok, 20B Swierkowa Street, Bialystok PL 15-950, Poland
COMMENT     ##Assembly-Data-START##
            Assembly Method :: GS Data Analysis Software v. 2.8
            Sequencing Technology             :: 454; Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000341665.1-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 03:47:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,892
            CDSs (total)                      :: 6,762
            Genes (coding)                    :: 6,518
            CDSs (with protein)               :: 6,518
            Genes (RNA)                       :: 130
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 121 of 244
            Pseudo Genes (incomplete)         :: 144 of 244
            Pseudo Genes (internal stop)      :: 62 of 244
            Pseudo Genes (multiple problems)  :: 66 of 244
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP004127.1.
            Bacteria available from Izabela Swiecicka (izabelas@uwb.edu.pl).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11331
                     /organism="Bacillus thuringiensis serovar thuringiensis
                     str. IS5056"
                     /mol_type="genomic DNA"
                     /strain="IS5056"
                     /serovar="thuringiensis"
                     /db_xref="taxon:1286404"
                     /plasmid="pIS56-11"
     gene            complement(19..177)
                     /locus_tag="H175_RS37395"
                     /old_locus_tag="H175_11p01"
     CDS             complement(19..177)
                     /locus_tag="H175_RS37395"
                     /old_locus_tag="H175_11p01"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425805.1"
                     /translation="MEMPKISLTYNLADVANGLTNWLGAYWPIIAFSVAIPLTFLVAFN
                     TKDLFTR"
                     /name="hypothetical protein"
     gene            complement(198..350)
                     /locus_tag="H175_RS37400"
                     /old_locus_tag="H175_11p02"
     CDS             complement(198..350)
                     /locus_tag="H175_RS37400"
                     /old_locus_tag="H175_11p02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001113828.1"
                     /translation="MPEFGFRPSMSQLFGNFHMNWSTMMPLLVILFGILFGFFVAHLVK
                     DKFDD"
                     /name="hypothetical protein"
     gene            complement(944..1249)
                     /locus_tag="H175_RS28010"
                     /old_locus_tag="H175_11p04"
     CDS             complement(944..1249)
                     /locus_tag="H175_RS28010"
                     /old_locus_tag="H175_11p04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001176491.1"
                     /translation="MQLMKVMLTGCKKVDFTNDNNERIEGVNIHFLTTTDNSEQSGFGY
                     LPSKVFFRKEFIEKFSSLNYPIVAEAIIAFNMSPRGIQTKISDFNILEEFPFTALK"
                     /name="hypothetical protein"
     gene            complement(1411..1893)
                     /locus_tag="H175_RS37870"
                     /old_locus_tag="H175_11p05"
     CDS             complement(1411..1893)
                     /locus_tag="H175_RS37870"
                     /old_locus_tag="H175_11p05"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_236635927.1"
                     /translation="MQGLEFWTRHELRLKKERADRAVKQLVVNDFSIEEMYFGIINNYI
                     SFRVVDKEDSNRNRWNIAPFWRQYIGQASKLRLAEALPVANVERTFKWLSHSVSKKLYL
                     LSEIFSQDDDTKKEFASYLENKGKQKITKGDLDLIENFPIRDFKEFLKIYNEKCLK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(1881..2480)
                     /locus_tag="H175_RS37875"
                     /old_locus_tag="H175_11p06"
     CDS             complement(1881..2480)
                     /locus_tag="H175_RS37875"
                     /old_locus_tag="H175_11p06"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_015413258.1"
                     /translation="MEKHIEKLSLSVCVDWLEFTFVYGEEFESICSFLGLDPTVFSKEI
                     DGFHKSYGYLSRYSFEEIHILMHGADNRSRIIMSGSGCRWFETLSSVGWYGLFDRIAFA
                     DEHGFSWIKVDRLDIAIDDFVGYFSVKKLKSKIKRRECLSRWKTSFVVETFDLEQGRLQ
                     GSTIYFGSASSKLRWVVYDKLEEQKKRIKIVIRFKV"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(2668..2859)
                     /locus_tag="H175_RS28020"
                     /old_locus_tag="H175_11p07"
     CDS             complement(2668..2859)
                     /locus_tag="H175_RS28020"
                     /old_locus_tag="H175_11p07"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000048809.1"
                     /translation="MSKVKGIRLNLRVSEDEYNKLQYVKECLLMEGLDLKDSAVIRYAL
                     LQTYKDYKEMNDLLKSRN"
                     /name="hypothetical protein"
     gene            complement(3186..3464)
                     /locus_tag="H175_RS28025"
                     /old_locus_tag="H175_11p08"
     CDS             complement(3186..3464)
                     /locus_tag="H175_RS28025"
                     /old_locus_tag="H175_11p08"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000263095.1"
                     /translation="MVYSYCVDCESVYNCEDEKCCCGSKKRSVDWEEFAFIMGYPLYPV
                     KKKYGVFHLDLSKRLDADALCDLKADNPELFYDEFKGWKERELKKRN"
                     /name="hypothetical protein"
     gene            complement(3478..3699)
                     /locus_tag="H175_RS28030"
                     /old_locus_tag="H175_11p09"
     CDS             complement(3478..3699)
                     /locus_tag="H175_RS28030"
                     /old_locus_tag="H175_11p09"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000391859.1"
                     /translation="MEELLHKIIDKLDKIDRDIQEIKTDLKAHRIETDDNFIKLYHLVE
                     EKDVEIDVLNKRLFKNEATVERIKREKH"
                     /name="hypothetical protein"
     gene            complement(3778..3993)
                     /locus_tag="H175_RS28035"
                     /old_locus_tag="H175_11p10"
     CDS             complement(3778..3993)
                     /locus_tag="H175_RS28035"
                     /old_locus_tag="H175_11p10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001169628.1"
                     /translation="MQKISEERKHRSFRFTDSELSLIDELVDIENDRRMTIAREMNLKY
                     KALNRTSFILYMAEERRRKYEEQGEI"
                     /name="hypothetical protein"
     gene            complement(4314..4649)
                     /locus_tag="H175_RS28045"
                     /old_locus_tag="H175_11p12"
     CDS             complement(4314..4649)
                     /locus_tag="H175_RS28045"
                     /old_locus_tag="H175_11p12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000457468.1"
                     /translation="MFAKYYSQDDEFMSPLQFIALGGIMSFLIKFGCISPSVNMLTLFV
                     IFVTCIGLCYFFLLLITKYFYRFLAKHRDNLHVYLCNKYNEKIANRIGLCIGLVYIVGV
                     LVICLLI"
                     /name="hypothetical protein"
     gene            complement(4661..5080)
                     /locus_tag="H175_RS28050"
                     /old_locus_tag="H175_11p13"
     CDS             complement(4661..5080)
                     /locus_tag="H175_RS28050"
                     /old_locus_tag="H175_11p13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000461785.1"
                     /translation="MFEKFYREYIHVLFNIVAMFFYFSWCLVGAEFNLLTLIAIGIALF
                     IFVKYVSMDKKFVNENIDKLKDKKMIFRKYAISGENIGYAVFTNFLLFLLYLGISVYFK
                     DLSVISLLFAFILNVSLLMLTLRLMSKFSLKNSKV"
                     /name="hypothetical protein"
     gene            complement(5092..5298)
                     /locus_tag="H175_RS28055"
                     /old_locus_tag="H175_11p14"
     CDS             complement(5092..5298)
                     /locus_tag="H175_RS28055"
                     /old_locus_tag="H175_11p14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000365466.1"
                     /translation="MDLPICNEKEVTVLSKTLKYLRSDQSNQVKVHKEYLEYRGWIFDD
                     LSYIDSHIYVVYKKEVVKRNKTF"
                     /name="hypothetical protein"
     gene            complement(5939..6130)
                     /locus_tag="H175_RS28060"
                     /old_locus_tag="H175_11p15"
     CDS             complement(5939..6130)
                     /locus_tag="H175_RS28060"
                     /old_locus_tag="H175_11p15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000511845.1"
                     /translation="MGILKKKRFREEVKRINKAHGEMREFLDLLMDRYGLDEEEINNCE
                     VIKHHFDNLDVMFSQMAK"
                     /name="hypothetical protein"
     gene            complement(6201..6803)
                     /locus_tag="H175_RS28065"
                     /old_locus_tag="H175_11p16"
     CDS             complement(6201..6803)
                     /locus_tag="H175_RS28065"
                     /old_locus_tag="H175_11p16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001102046.1"
                     /translation="MNVMVFEGPLGSGKTLGMTLFAHHFKQKSNCVLYSNYGVVGSKPF
                     TEIEHFKNIAQEKSTILNLDEAHIDLDARSFSSNSVKFFSQVSYYLRKLRCTLFIASPS
                     FDDLDARIRGITNVLVKVSSDKKYFYYTMYDIQSKRYLKRMRISKKKAFVVGSKIYDTS
                     AMVSPVKVPEKRQDFMEFLEALKTTAEEYGRQYKHSA"
                     /name="hypothetical protein"
     gene            complement(6800..7207)
                     /locus_tag="H175_RS28070"
                     /old_locus_tag="H175_11p17"
     CDS             complement(6800..7207)
                     /locus_tag="H175_RS28070"
                     /old_locus_tag="H175_11p17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504993.1"
                     /translation="MGEVTLEKTVVEEVLPDVQDIMKEEILEEDTSSESIETVQLFQYM
                     KENSVPLNASQMKGILLLNENGCKDIADYVISVKKQSLPLAFYEKVLKILTMFDRIKGN
                     AKLSHLLKANANPSLSLSANSAKVVKGNELA"
                     /name="hypothetical protein"
     gene            complement(7221..7583)
                     /locus_tag="H175_RS28075"
                     /old_locus_tag="H175_11p18"
     CDS             complement(7221..7583)
                     /locus_tag="H175_RS28075"
                     /old_locus_tag="H175_11p18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000880240.1"
                     /translation="MLAKTKRKVQPQFEGHRALIIKEEGTCVVEQVISFDSEILETSTA
                     MLPRDNELRLLDDNHGYVYHIYNLEKPARIEAENIKRLRRSSALKGIFEFETGKKQFDL
                     IGFMPWVIIVLLAIFK"
                     /name="hypothetical protein"
     gene            complement(7890..8150)
                     /locus_tag="H175_RS28080"
                     /old_locus_tag="H175_11p20"
     CDS             complement(7890..8150)
                     /locus_tag="H175_RS28080"
                     /old_locus_tag="H175_11p20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000992627.1"
                     /translation="MNALIDTIFNPVLGWLAQMAKYLNDLSVPASRPLRISDYLGVFAY
                     LYGWTTVITTLFALVFVYFVCYIIMTYSSLFIRFKDTVKWW"
                     /name="hypothetical protein"
     gene            complement(8150..8575)
                     /locus_tag="H175_RS28085"
                     /old_locus_tag="H175_11p21"
     CDS             complement(8150..8575)
                     /locus_tag="H175_RS28085"
                     /old_locus_tag="H175_11p21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000970223.1"
                     /translation="MMEKFVKFLKGGFESVIKSLKTFIDFLATPFQYLLDFLKGIFYFI
                     EKLFQVVVAIIKLFVALFQFLGAVIAGFFKTLFLWVQPNFDGGNVFFPSASMKGFKTAT
                     DILLPTGLLTVVPMVVTCFIWYLFVKKLMDLFRGGHH"
                     /name="hypothetical protein"
     gene            complement(8572..8727)
                     /locus_tag="H175_RS37405"
     CDS             complement(8572..8727)
                     /locus_tag="H175_RS37405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015386302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_169309952.1"
                     /translation="MIQPVTSKDLIQFLQYAFHAGENVLFLAFVLMITFSVAVSIRRIM
                     VGYKQS"
                     /name="hypothetical protein"
     gene            complement(8737..9846)
                     /locus_tag="H175_RS28090"
                     /old_locus_tag="H175_11p22"
     CDS             complement(8737..9846)
                     /locus_tag="H175_RS28090"
                     /old_locus_tag="H175_11p22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_003275181.1"
                     /translation="MPVLFGILFVSNTSVFAEELPEYVGDKIVGKVYYRNDPTGFPLRL
                     VNEGRNGRLEFSKPVDIAGFSYSISNMKSSFLVRVYYQGNIYSKDFLFKQGETSKSFVE
                     GVLKGVSKVEFQAYSYDGLTLNYLNFFEYRQPPPNDVSDIKIENVTHDSVKLTYKFPTE
                     NFSNVKVFRDGKVIANDVKTEYFTDKGLSPETEYTYKFVSVSPSGNQESKGIEYKVKTE
                     QAPDLTKPAKPSSPIVTPKDGSLIVNLTNYNAGVKIKGYHIIVDGKQVNDSLVTGRSYA
                     IKGLKNGQSYQVQIKSVSAWNVESDLSNSVPGIPQVQVIPDIAFNFGLTDLIVSIKNWF
                     GGIWPIVAFSIAIPLAFIVAFNTKKLFLR"
                     /name="hypothetical protein"
     gene            complement(9864..11243)
                     /locus_tag="H175_RS28095"
                     /old_locus_tag="H175_11p23"
     CDS             complement(9864..11243)
                     /locus_tag="H175_RS28095"
                     /old_locus_tag="H175_11p23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000708825.1"
                     /translation="MKKAAIIFLCTFCIIFRTVPVFADTPSFQMVGSKGVSIENKGLVG
                     GYYSLSLKISDAKDVVTFQLSELMLVNDCSFNFGLVKWTRPSQEKNEFDIKVKENGTYA
                     FYVFVNGKVKGYVRFKIKGFPKNAEGTGVTQYWGQGAKYYELPEEHVNPEDFRDKDGEN
                     NGICVNEKIPDDKPGGSGDIEEPKEDNKPPVEGGGSDKAIKEALDKITSAVNEISKNTG
                     ETAKNTGEIKKNTDEIAKNTKEIADAVKDIRDNLKIPDDMDLSIKPVLPEDIKPDKPKQ
                     PDKPFEDKQEHFKEGEQEKPPDKLPVAPEPAECWGDVCKDKDNQKEEDMKKEKDMDKEE
                     PMKPDKPMEKEDMKPDKPMTPDKPMEKEDMKPDKPMTPDKPMEKEDMKPDKPMTPDKPM
                     EKEDMKPDKPMTPDKPMDREGEMKKDNDMKQDKPMAPWEEMKPSDEGSELCWHVVNGKC
                     G"
                     /name="hypothetical protein"
     Typing          complement(1412..2480)
                     /name="rep_cluster_814"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.907"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1906..2480)
                     /name="MOBT"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.958"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aggagtgatt tttgatgatt aacgagtaaa taaatcttta gtgttaaacg ctactaagaa
       61 cgttaacgga atagcaacag agaatgcgat aataggccag taagcgccta accagttagt
      121 taaaccattt gcaacatctg caagattata agttaatgaa atttttggca tttccaaaat
      181 aaattcctcc tagttaatca atcatcaaat ttgtctttta ctaaatgcgc tacgaaaaat
      241 ccgaacagta tcccaaacaa aatgactaac aatggcatca ttgttgacca attcatatga
      301 aagttaccga ataattgaga catcgatggt ctgaatccaa attcaggcat tgaaatcaac
      361 tcctaaaggt gttgtacact ttaccaatac agtagatagc aaacgcccca ccaatgaata
      421 aatagatcac tggtaacact gaactaaaca tgttgaaggc atactgcaag attcttccca
      481 cgtccatgct aaaactaata gccatcaccc caagctttga cggtggtgaa catcattatt
      541 acgaacacaa gaaaggttat aaaacctaac gaatataata caactgaatt attcatgaag
      601 aaccaataca acatatctag aaacgctaaa tcattcatta tctatcaaac cgccaatctt
      661 tcagcaatgt gaacacgata attaataaaa atggtaatcc aaacaaggaa tattgattca
      721 acaagttttc taaattaaac tcgatcatcg taaagcactc catatccagt tgaatgtcaa
      781 caccaacaag attaacaata acaagaaact aattaacata tcaccatagg taagtgtctg
      841 gaaaactgca aaactaccat tcttagtctg cactaagtaa ttaggcataa tttccgaaat
      901 agttttgtac aattcattta aatcgatttt atctatcata aaatcacttt aaagctgtaa
      961 aaggaaattc ctctaaaata ttaaaatcag aaattttagt ttgaatacca cgaggagaca
     1021 tattaaaagc aataatagct tcagcaacaa ttggataatt caaagaacta aacttttcta
     1081 taaactcttt tctaaaaaag accttagaag gaagatatcc aaaaccagat tgctcactat
     1141 tatcagttgt agttaaaaag tgaatattaa caccttcgat tctttcatta ttatcatttg
     1201 tgaaatcaac tttcttacaa cctgttaaca ttactttcat taattgcata tttacactcc
     1261 ttagaatctt ttttagtcat acaatgtcgt tttatgtaca cctttgtcat acatttaatt
     1321 ataagaaaaa ctagaaaaaa attctagtct ttttttaaaa atatatggat tttttaatgt
     1381 attagacttc cattataaaa tagagtaaaa ttattttaga catttttcat tataaatctt
     1441 taaaaattct ttgaagtcac gaattgggaa attttctatt aagtctaaat cccctttcgt
     1501 gattttttgt ttgcccttat tttctaagta actagcaaac tctttctttg tatcatcatc
     1561 ttgcgaaaaa atttcagaca acaaatataa ttttttagaa acactatgag acaaccattt
     1621 aaacgtacgc tcaacatttg caacaggtaa agcctctgct aatcttaatt tacttgcttg
     1681 tcctatatat tgcctccaaa aaggtgcaat attccaccta tttctattag aatcttcctt
     1741 atcgacaaca cgaaacgaaa tataattatt tataatacca aaatacattt cttcaatact
     1801 aaaatcatta actacaagtt gtttaacagc acgatcagca cgctcctttt tcaaacgcaa
     1861 ctcatgccta gtccaaaatt ctaaaccttg aatcgaatca ctatctttat tctttttttc
     1921 tgctcttcta acttatcata aacaacccat ctcaatttac tagaagcact tccaaaataa
     1981 atcgtagaac cctgcaatcg tccctgttct aaatcaaaag tttcaacaac aaaacttgtt
     2041 ttccaacgac tcaaacattc tcttcttttt attttagact ttaatttttt aacagaaaaa
     2101 taacctacaa aatcatcaat agcaatatct aaacggtcaa ctttaatcca agaaaaacca
     2161 tgctcgtctg caaatgcaat cctatcaaac aaaccatacc agccaacaga ggataatgtt
     2221 tcaaaccatc tgcaaccaga accagacata ataattctag aacgattatc agcaccatgc
     2281 ataagaatgt gtatttcttc aaaagaatat ctagacaaat aaccataaga cttgtgaaaa
     2341 ccatctatct ccttagaaaa aacagtagga tctagaccta aaaaactaca aatagattca
     2401 aactcttcac cataaacaaa agtaaactcc aaccagtcca cacaaaccga aagagacaat
     2461 ttttcaatat gtttttccat acaaacacct ctaaaacaat acaataaaag caaaataaaa
     2521 accgcgaatt ttttgcctaa ttttttaaaa gaaccgtgaa aaaaacaaag agggacgaac
     2581 cctagagcca caagcgtttg ggggcgattg acctgtattt tctactgggg tgttactagc
     2641 ccccagtaga aacatgaaat cagccattca attccttgat ttcaacaaat cattcatttc
     2701 tttataatcc ttataagtct gtaataaagc ataacgaata acagctgaat ctttaagatc
     2761 aagaccttcc ataagtaaac attctttaac atactgcaac ttgttatact catcttcaga
     2821 aactcttaaa ttcaaacgaa tacctttaac ttttgacatt atataacctc ctcaattgaa
     2881 gcatacaatg tcatacacca tatgtaaaga gtttccattt tcctccttgt aaatattata
     2941 ccaatatata aatcttttaa ccttcattaa aaaatggaaa ttttatcaat gattttccgt
     3001 gcattttttt gtgaattctg tacagattta aaagtgacct gaacccagtc tagggaaggg
     3061 atagtgtaaa tatcagatgg cccgggaacc cccgtgttac tggacgggag ttcctcattt
     3121 cgcaactgga aattttcctt cgaattagac aaacaaaaca cccactaact ccaaaatatt
     3181 tattattaat ttctcttttt caattctctt tctttccaac ctttaaactc atcataaaaa
     3241 agttctggat tatctgcttt aagatcacat agagcatcag catctaaacg tttagataaa
     3301 tctaaatgaa aaacaccgta ttttttctta acaggatata acggataacc cataataaaa
     3361 gcaaactctt cccaatcaac agaacgtttt ttacttccac aacaacactt ttcatcttca
     3421 cagttataca cactctcaca atccacacaa taactatata ccatcgacaa tttcctccta
     3481 atgcttttcc ctcttaatac gttcaactgt cgcctcattc ttaaatagac gtttattcaa
     3541 tacatcaatc tcaacatctt tttcctcaac taaatgatat aacttaataa aattatcatc
     3601 agtttcaata cgatgagcct tcaaatctgt tttaatctcc tgaatatccc tatcaatctt
     3661 atctaattta tcaataattt tatgtaacaa ttcttccata attaaaacct cccaaattta
     3721 atcttaaaaa agtgcgactc tatccctatt attattaact agttaagaga aaaccactta
     3781 aatctcccct tgttcttcat atttacgtct tctttcttca gccatatata aaataaaact
     3841 tgtacgattt aatgctttat attttaaatt catttctctt gcaattgtca ttcgtctatc
     3901 attttcaata tcaactaatt catcaattaa tgacaactca ctatcagtaa aacgaaatga
     3961 cctatgtttt ctttcttcac ttattttttg cataattaaa acctcctata aaactatcgt
     4021 atacacaata taacacatta aaattacaaa aacaaccttt gtatacacaa aaaaataaaa
     4081 gtgtacacaa aggcgtgtga tcaaatcatt tttttaaact tttttaatac atttcaaaac
     4141 aatattttta aaataaactc taacaaaata ccaaattaac aatatcaaaa atcctgtcgt
     4201 aaaaataaac aaatcactat atatatcaga tacatacttt gttgtaacaa cacagatcat
     4261 tgcaagcata catatatcaa aaactacagt taaccaaaat ttaaaattca actttaaatc
     4321 agcaaacata taactaacac ccctacaata taaactaaac caatacataa accgattcta
     4381 tttgctattt tttcattgta cttattacat aaataaacat gtaaattatc acgatgttta
     4441 gcaagaaatc tataaaaata ctttgttatt aaaagtagaa aaaaataaca taatccaata
     4501 caagtaacaa aaataacaaa taacgttaac atattcacac taggcgaaat acaaccaaat
     4561 ttaataagaa aactcataat accacccaaa gctataaatt gaagtggcga cataaattca
     4621 tcatcttggc tataatactt agcaaacata tatacacctc ttaaacttta ctattcttta
     4681 aactaaattt actcattaat ctaagagtca acataagcaa actaacatta agaataaaag
     4741 caaataaaag cgatattaca cttaaatcct taaaatatac cgatatccct aaatacaaaa
     4801 gaaacaataa aaaatttgta aaaactgcat atccaatatt ttcaccactt atagcatatt
     4861 tcctaaaaat catctttttg tcttttaatt tatctatatt ttcattaaca aatttcttat
     4921 ccatagatac atacttaaca aaaataaaaa gagcaattcc aattgctatt agagttaata
     4981 aattaaattc agcaccaact aaacaccaac taaaataaaa aaacatcgca acaatattaa
     5041 acaatacatg aatatattca cgataaaact tttcaaacat atatatcccc cttaaaacgt
     5101 cttatttcgc ttcacaacct cttttttata aactacatag atatgactat ctatataact
     5161 taaatcgtca aatatccaac ctctgtattc taaatactcc ttatgtacct taacttggtt
     5221 agattgatca cttctcaaat atttcaacgt tttagataaa acagttacct ctttttcatt
     5281 acaaataggc aaatccatta catatcaccc ttcttttaac gtgtctaaca tgcctttaaa
     5341 caagaaacaa atccctagag taccaaacaa cgtaacaata acattttcca tcaaaacaac
     5401 cctctatctg catttgctaa aaggttaaac aagaaacagg tagtattttc ttacgctctg
     5461 cttcataaaa tctccacctt tttccgctgc gctacattct tgcataactt ttttaggctc
     5521 gtttgtcttc atcgccaagt cgataaactc aaaagtgaat atatttaact atcaacttgc
     5581 ctatccatta tacgagccta aacgcttgta ctaccgacct ttatattttc ttttattcta
     5641 tttatccatt acgctatgtg gggctagccc cacaccccag ccaatctcca acctcaaaag
     5701 cgaaggaaga cctttaaaac ctaacccgta aaccaattta acaagaaaac aattacaaca
     5761 ataaatgaac cataaaataa tccatctctc gccatacttt tcaaccaatc tacatgtatc
     5821 atttcaactt tttatcctct ctaataaata tgcatatcac taactaataa ataagcactc
     5881 ccactcacaa ctaaccctaa gacagtacca aatacaatac acgcaaacat ttccatacct
     5941 attttgccat ttgactaaac atcacatcta aattgtcaaa atgatgtttg attacttcac
     6001 aattatttat ttcttcttcg tctaatccat agcgatccat aagcaaatct agaaattctc
     6061 tcatttctcc atgtgcttta tttattcttt tcacttcttc acgaaatctt ttcttcttta
     6121 aaatccccat aacaacgttc cttccttaat ataattttta taatcttatg aagtcctccc
     6181 gcctgtaccg cgcatacagc ttacgctgaa tgcttgtact ggcgtccgta ctcttcggcg
     6241 gtcgtcttca acgcttctaa aaattccata aagtcttggc gtttttctgg aacttttact
     6301 ggcgaaacca ttgcacttgt atcataaatt tttgaaccaa caacaaaagc tttcttttta
     6361 cttatacgca ttctctttaa ataacgttta gattgaatat catacatcgt atagtagaaa
     6421 tattttttat cgcttgaaac tttaacaaga acgttcgtaa tacctcgtat acgtgcatct
     6481 aaatcatcaa aactaggcga agcaataaac aacgtacaac gtaattttcg aagataatac
     6541 gatacttgag aaaagaattt aaccgaatta ctcgaaaaac tccgagcatc taaatcaatg
     6601 tgagcttcat ctaaatttaa aattgtagat ttttcttgag caatattttt aaaatgttct
     6661 atttcagtaa atggctttga accaactacg ccataattgc tatacaatac gcaattactt
     6721 ttctgtttaa agtggtgtgc aaacaatgtc attcctaatg tcttaccgct tccaagagga
     6781 ccttcaaaaa ccattacgtt catgcaagtt catttccttt cactactttt gcactattag
     6841 ctgaaagtga taatgatgga ttcgcattcg ctttcagtaa atgacttaat ttcgcattac
     6901 ctttaattct gtcaaacata gttaatattt ttagaacctt ttcatagaag gcaagtggta
     6961 aactctgttt tttcacagag ataacataat cagctatatc tttacatcca ttctcattaa
     7021 gcaataagat acctttcatt tgagatgcat tcaaaggcac tgaattttcc ttcatgtatt
     7081 gaaataattg aaccgtttca atactttcac ttgaagtatc ttcttctaaa atttcttctt
     7141 tcattatgtc ttgaacgtct ggtaacactt cttctacaac tgttttttct aaagtcactt
     7201 cacccattca tttcacccca ttatttaaaa atagctagta aaacaataat tacccatggc
     7261 ataaaaccaa ttaaatcaaa ttgttttttt cctgtttcaa attcaaaaat cccttttaac
     7321 gcactagatc gtcgtaatct tttaatattt tccgcttcaa ttcttgccgg tttttctaag
     7381 ttataaatgt gatacacata accatgatta tcatcaagta atctcaattc attatcacgt
     7441 ggtaacatgg ctgtactcgt ttctaaaatc tccgaatcaa acgatataac ttgttcaact
     7501 acacatgtac cctcttcctt aataattaac gctctatgtc cttcaaattg tggctgtact
     7561 tttcgtttcg ttttagctaa catttcaatc actctctctt tttaattcca ttttcttttt
     7621 aaccacatac aaggtaacac tataaataga aatacagata aacaaattgc acttgataac
     7681 tcaaacattg cattaaaagc ctgcattttt agctctcctt tttcgaagtt gcatcacaaa
     7741 ctaaaattaa taaacaacta acgactccca atattaaata aatcattctt ctatacctct
     7801 aaagaatttc aatgtatcta ttaccatcca agaaacaact gttgtaacaa gtattaataa
     7861 aattgcttgt cctacaatat tcaatatcat taccaccatt tcactgtatc tttaaatcga
     7921 ataaataaac tactgtaagt catgatgata taacaaacaa aatatacgaa aactaaagca
     7981 aataacgtcg ttataacggt tgtccaaccg tataaataag caaacactcc aaggtaatca
     8041 ctaattcgca aaggacgact tgctggtacg cttaaatcat ttaaatactt tgccatttgc
     8101 gcaagccaac caagaacagg attaaatatt gtatcaatta acgcattcat taatgatgac
     8161 cacctctgaa tagatccatg agctttttaa caaacaagta ccaaataaaa catgtgacaa
     8221 ccatcggaac tactgttaac aaaccagtag gaagtaatat atctgtcgct gtcttaaatc
     8281 ccttcatcga agcactagga aaaaatacat tcccaccatc aaaattaggt tgtacccata
     8341 gaaataaagt tttaaagaat cccgctatta cagcacctaa aaactgaaac aaggcaacga
     8401 ataattttat aattgctact acaacttgaa acagcttttc tatgaaataa aagattcctt
     8461 ttaaaaaatc cagtaaatat tgaaacggtg tagctaaaaa atcaataaac gtttttaacg
     8521 actttataac cgattcaaat ccacctttta agaacttaac aaacttctcc atcatgattg
     8581 cttataaccc accataatcc ttctaatgct tacagcaaca gagaatgtaa tcattaatac
     8641 aaacgctaga aataatacgt tctctccagc atgaaatgca tactggagaa attgtattaa
     8701 gtccttagat gtaactggtt gtatcaaact aagcacctac cttaaaaata acttcttcgt
     8761 gttaaacgca acaataaacg ctaatggaat agcaatacta aacgcaacaa taggccaaat
     8821 tccaccgaac caatttttaa tagatactat taaatcagtg agaccaaaat taaatgcaat
     8881 atcaggtatt acttgcactt gtggaatacc tggaacagaa ttactcaaat cactttctac
     8941 attccaagcc gatacagatt taatctgaac ttgatacgat tgtccattct ttaacccctt
     9001 aatagcataa gaacgccctg taacaagcga atcattcact tgtttaccat ctacaataat
     9061 gtgataacct ttaattttta cacctgcatt ataattcgtt aaattaacaa ttaaagaacc
     9121 atcttttggt gtaactattg gactagatgg tttagctggc tttgttaaat caggagcctg
     9181 ttcagtttta actttatact caatgccttt tgattcttga ttaccactag gcgagacaga
     9241 aacaaactta taagtatatt cagtttcagg gcttaaaccc ttatcagtaa aatattcagt
     9301 tttaacatca ttggcaatta ctttcccatc cctaaatact tttacattac taaaattttc
     9361 agttggaaat ttataagtta gctttacaga atcatgtgta acattttcta tttttatatc
     9421 cgaaacatca tttggtggtg gctgtcgata ttcaaagaaa ttaagataat tcaaagtcaa
     9481 accatcataa ctgtatgctt gaaattcaac tttagaaact ccttttaaaa caccttccac
     9541 aaaactctta gatgtttctc cttgtttaaa aaggaaatcc ttcgaataaa tattcccttg
     9601 ataataaacc cttactaaaa aactagattt catattagaa atagaataag aaaaccccgc
     9661 aatatcaact ggctttgaaa attctaatct tccatttcgt ccttcattca ccaaacgaag
     9721 agggaatcca gtgggatcat tacgataata tactttcccg acaatcttat cccctacata
     9781 ctctggtaat tcctcagcaa atacacttgt attacttaca aataaaatcc caaataatac
     9841 aggcactatt aataaaaatt tctttatccg cattttccat ttactacatg ccaacaaagt
     9901 tcactcccct cgtcacttgg tttcatctct tcccaaggag ccattggctt atcttgtttc
     9961 atatcattat cttttttcat ttctccctcc cgatccatag gcttatcagg agtcattggt
    10021 ttatcaggct tcatgtcttc cttttccatt ggcttatcag gagtcattgg tttatcaggt
    10081 ttcatgtctt ccttttccat cggcttatca ggagtcattg gtttatcagg tttcatgtct
    10141 tccttttcca tcggcttatc aggagtcatt ggtttatcag gtttcatgtc ttccttttcc
    10201 attggtttat caggcttcat tggttcttct ttatccatat ctttttcttt cttcatatct
    10261 tcttctttct gattatcttt atctttacaa acatcccccc aacattcggc gggctcagga
    10321 gcaacaggaa gtttgtcagg tggtttctct tgttcacctt ctttaaaatg ctcttgtttg
    10381 tcttcaaaag gcttatcagg ttgtttaggt ttatcaggct ttatgtcctc aggaagaaca
    10441 ggtttaatcg acaaatccat atcgtcagga attttcaaat tgtccctaat atccttaaca
    10501 gcatccgcga tttcttttgt gttctttgct atttcatcgg tgttcttttt aatctcacct
    10561 gtatttttag ctgtttcacc cgtattcttt gaaatttcat ttactgcact tgtaatttta
    10621 tctaaagctt ctttaatagc tttatcagaa ccaccacctt caactggtgg tttattatcc
    10681 tcttttggtt cttctatatc tcctgaacca cctggtttat catcaggaat tttttcattc
    10741 acacaaattc cattattttc tccatctttg tctcgaaaat cctcaggatt tacatgttct
    10801 tcaggaagtt cataatactt tgcaccttgc ccccaatatt gagttactcc agtaccttca
    10861 gcatttttag gaaatccttt aattttaaaa cgaacatatc ctttaacttt cccattcaca
    10921 aacacgtaaa aagcatatgt tccattttct ttgactttaa tatcaaactc atttttctct
    10981 tgtgacggcc tagtccattt cactagtcca aaattaaaac tacaatcatt aacaagcatc
    11041 aactcactca attgaaacgt cactacgtct ttagcatcac taatctttaa cgaaagactg
    11101 taataaccgc caacaagccc tttattttct atggaaaccc ctttacttcc taccatttga
    11161 aatgatggcg tatcagcaaa gacaggaact gtacggaaaa taatacaaaa tgtacataaa
    11221 aaaattatgg ctgctttttt catatcaatc cttcctaaca aaaaatagct aggcgatcca
    11281 cccagctatt tgattgtttt attaaattta tagttaactt cttgctattt a
//
