LOCUS       NC_020390               6880 bp    DNA     circular CON 07-APR-2024
DEFINITION  Bacillus thuringiensis serovar thuringiensis str. IS5056 plasmid
            pIS56-6, complete sequence.
ACCESSION   NC_020390
VERSION     NC_020390.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02604143
            Assembly: GCF_000341665.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis serovar thuringiensis str. IS5056
  ORGANISM  Bacillus thuringiensis serovar thuringiensis str. IS5056
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Murawska,E., Fiedoruk,K., Bideshi,D.K. and Swiecicka,I.
  TITLE     Complete genome sequence of Bacillus thuringiensis subsp.
            thuringiensis strain IS5056, an isolate highly toxic to Trichoplusia
            ni
  JOURNAL   Genome Announc 1 (2), e0010813 (2013)
   PUBMED   23516221
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Murawska,E., Fiedoruk,K., Bideshi,D.K. and Swiecicka,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2013) Department of Microbiology, University of
            Bialystok, 20B Swierkowa Street, Bialystok PL 15-950, Poland
COMMENT     ##Assembly-Data-START##
            Assembly Method :: GS Data Analysis Software v. 2.8
            Sequencing Technology             :: 454; Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000341665.1-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 03:47:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,892
            CDSs (total)                      :: 6,762
            Genes (coding)                    :: 6,518
            CDSs (with protein)               :: 6,518
            Genes (RNA)                       :: 130
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 121 of 244
            Pseudo Genes (incomplete)         :: 144 of 244
            Pseudo Genes (internal stop)      :: 62 of 244
            Pseudo Genes (multiple problems)  :: 66 of 244
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP004124.1.
            Bacteria available from Izabela Swiecicka (izabelas@uwb.edu.pl).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Bacillus thuringiensis serovar thuringiensis
                     str. IS5056"
                     /mol_type="genomic DNA"
                     /strain="IS5056"
                     /serovar="thuringiensis"
                     /db_xref="taxon:1286404"
                     /plasmid="pIS56-6"
     gene            complement(join(6745..6880,1..1181))
                     /gene="mobV"
                     /locus_tag="H175_RS27885"
     CDS             complement(join(6745..6880,1..1181))
                     /gene="mobV"
                     /locus_tag="H175_RS27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099255.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_001031673.1"
                     /translation="MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNY
                     DLLNQQKIRYESTIKQEISERVKRKPRANSVVLSEFVVTASPDYMHSLRLEEQKRYFES
                     SLVFIQKRYGKQNTLYAMVHMDEATPHMHIGVMPITEDNRLSAKDMFTRKELISLQQDF
                     PLEMREKGFDVERGEGSEKKHLSPQAFKEKQDLEIEVKQLSNVKTHLKTKVVETHNQLQ
                     QTTNYIEKQNETLQKIQQQFLSLDKKIKEKKQEFEMFRNQIPDKSVSMSYLREETKTEV
                     TTKLFGKPEIIEKKTGNIVVTREQWRDMTEKVNAAVIIKSDYESLQKTDLVKENKQLHE
                     AVDGICDSLQDSQKRNLKLQEENKQLRTEISSLKAHIRDLQINIKVLYQQTKKVFKEQF
                     KAFRGLIKNELDIKDVDNQFEREHAREVKSRQKGYDMER"
                     /name="mobV"
     gene            complement(1594..1890)
                     /locus_tag="H175_RS27895"
                     /old_locus_tag="H175_6p01"
     CDS             complement(1594..1890)
                     /locus_tag="H175_RS27895"
                     /old_locus_tag="H175_6p01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000043575.1"
                     /translation="MSKQQVKFYVMEKEMERLKLLSEMRYMTVPNYAKLTALGVQIRQP
                     KEIVVGNVELQEEDKAILKEILERRRGISYIAYDTGFNQRLEQLAKRLLGEEG"
                     /name="hypothetical protein"
     gene            complement(2434..3048)
                     /locus_tag="H175_RS27900"
                     /old_locus_tag="H175_6p02"
     CDS             complement(2434..3048)
                     /locus_tag="H175_RS27900"
                     /old_locus_tag="H175_6p02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133991.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001133991.1"
                     /translation="MPNTIEFKQVEKNARIRDLEIENEKFKQDHHEVSKDELEKAMETM
                     AKATGKELYIGTKKSPHSRVRFAQSLQENLGFLNKNGYLTNKEKVFLSDITPYIAFSSN
                     CIVHDIKAKNPVPANVSEIAKLIGISRQNTSLAINSLVKKGLLFKGDSGVEGNNAKAYA
                     VFVNPHIIYAGDKDSVNEALQVMFYKAMKMKILKDLPDKLF"
                     /name="helix-turn-helix domain-containing protein"
     gene            3502..4596
                     /locus_tag="H175_RS27905"
                     /old_locus_tag="H175_6p03"
     CDS             3502..4596
                     /locus_tag="H175_RS27905"
                     /old_locus_tag="H175_6p03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000383146.1"
                     /translation="MDVQTKSNEQITNMLNDWYIEIRARHLGNAHKLRLEIDKKIHNIE
                     EDQNLLLYYSLLDFRHQYLIDHLSIGKDSFDKIESFNTPTDNLLSYYYFFFKAIHATSI
                     GNYNLAREYYDKAEIKLKEIPDQLEHAEFYYKLSTFSCHNQQYVPAIKQASKAKEIFSK
                     YKGYELNIGYCNNLSGLSCTHLKEYELAEEYFISAIDIFQKEKEEQAILYVRHNLGFMY
                     AKQNLSELAIRYLSEVIQKSPNNYRAILIEACEHAKLNNKNKALELLEKGLKISNNLKI
                     EEYQHRFKILLAINNEIPGEKLEQIILAGMIYFEKENLYEYIAEYNETLAIKFYHEDNH
                     SKASKYFYLSSKARKKSHNKGALK"
                     /name="hypothetical protein"
     gene            4593..4733
                     /locus_tag="H175_RS37390"
                     /old_locus_tag="H175_6p04"
     CDS             4593..4733
                     /locus_tag="H175_RS37390"
                     /old_locus_tag="H175_6p04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000751237.1"
                     /translation="MKKTILTLMGIITVFTLTLSNINTPKENKDPSIQKIMLMSDGNTG
                     G"
                     /name="hypothetical protein"
     gene            complement(4798..5064)
                     /locus_tag="H175_RS27910"
                     /old_locus_tag="H175_6p05"
     CDS             complement(4798..5064)
                     /locus_tag="H175_RS27910"
                     /old_locus_tag="H175_6p05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000144827.1"
                     /translation="MTFVMIQIGIAIFLLFIIGLIYKVIGKKFNSKFSMYFLVIIVFFA
                     LLFLVISEINTNGFTDLKVLVFLPLIVLSLILLIINAIKSKKI"
                     /name="hypothetical protein"
     gene            complement(5091..5834)
                     /locus_tag="H175_RS27915"
                     /old_locus_tag="H175_6p06"
     CDS             complement(5091..5834)
                     /locus_tag="H175_RS27915"
                     /old_locus_tag="H175_6p06"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003308357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000782444.1"
                     /translation="MKMKKLGAGLLSAALLFPVVAPVVSHAEENTTVSEKNQIQETTKK
                     YFKSVEGMGTQYTEKQSKEIIDSVAIVSKYIKPNEHNQLELDSKVKEQVSPFVYEHYQR
                     GIQAINQATEAGYATINVENNKLEATPLANSSMNNLVGFNAKDGIQYSSVDSYWWGVKW
                     YMSKSESQQWQNNFADGSFYWGSIAAITGLLTPFFPPAAIASAVSVIMSVGNYHCYREL
                     RDHTSSRGSVMTISWLDKEIYANKR"
                     /name="hypothetical protein"
     Typing          complement(7..1181)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.898"
                     /coverage="89.0"
                     /evalue="0.0"
ORIGIN
        1 acttttttcg tctgttgata taaaaccttt atattaatct gcaagtctct tatatgagcc
       61 tttaacgagc ttatttctgt tcttaattgt ttgttttcct cttgtaactt taaatttctc
      121 ttttgagaat cttgaagtga atcgcatatt ccatcgactg cctcatgtaa ttgtttgttt
      181 tcttttacaa gatccgtttt ttgcaaactt tcataatcgc tctttataat aaccgctgca
      241 ttcacttttt ctgtcatatc tctccattgc tcacgtgtaa ccacgatgtt tcctgttttt
      301 ttctctatga tttctggttt tccaaacagt tttgttgtta cttctgtttt tgtttcttct
      361 cgcaaataag acattgacac tgacttatcc ggaatttggt ttcgaaacat ttcaaattcc
      421 tgtttctttt cttttatttt tttatctaaa ctcaaaaatt gttgctgaat tttttgtaac
      481 gtttcattct gtttttcgat gtagtttgtg gtttgttgta attgattatg cgtttccact
      541 acttttgtct ttaaatgcgt ttttacattg gataattgtt tcacttctat ttctaaatct
      601 tgtttttcct taaaggcttg aggcgataaa tgttttttct ctgaaccttc tccccgctct
      661 acatcaaaac ctttttcccg catttccaac gggaagtcct gttgtaaaga tatgagctct
      721 tttctcgtaa acatatcttt tgcggacaaa cgattgtctt ctgtaattgg catgacacca
      781 atatgcatat gtggtgtcgc ttcatccata tgaaccatcg catacaacgt attttgtttc
      841 ccataacgtt tttgaataaa cactagtgaa ctctcaaaat aacgtttctg ttcctctaaa
      901 cgtaatgaat gcatgtaatc aggcgaagct gttactacaa attctgaaag tacaaccgaa
      961 ttggctcgtg gctttcgttt tactcgttca ctgatctctt gtttaattgt gctttcatat
     1021 ctaatttttt gctgattcaa caaatcataa ttttgttcac ttttcgaata atcaatatcc
     1081 aaattggatt tactttttcc ttgtctctga ttatgttttt gaattccttg cacatcagaa
     1141 atttgaaatt tctgcatgtg aatgattgcg aatttgttca tgtttttccc tcacaattgt
     1201 cttccgtaag aggactactt ttgagactat cataacatat tgagagagcg aatccaaagt
     1261 tttggtatag cccactatac caaattccat ttggtgtggg ctctgcgaga ccttgttggc
     1321 ttcgccaaat cacgccacag cgtgataaac accaggggct ttgccccaaa ccccacaagg
     1381 ggaactcttc cccttgaccc caccaatcga actccccaac ccctcaatcc ttgaggggct
     1441 ccctcgccgg ttggcgtccc caaaacgatt cgttttgagg aactgccaag aggatggaga
     1501 atcggctgca cgtgtccatt ccttaatgct cctttcaggg atcagcataa accgctgatc
     1561 ccttccagtc cgcttaccat tgacacttac cgcttatcct tcttctccca atagtcgttt
     1621 tgcgagttgt tccaatcgct ggttaaatcc tgtgtcataa gctatataac taattcctcg
     1681 tcttctctcc aaaatttctt ttaatatcgc tttatcttct tcttgtaatt ctacattgcc
     1741 tacaactatc tcttttggct gtctaatttg cacacctaat gctgttagct ttgcataatt
     1801 aggaactgtc atatatcgca tttcagacaa aagttttaaa cgctccattt ccttttccat
     1861 aacatagaat ttaacctgtt gtttagacat tcacacgacc tccaataaac taaatagtac
     1921 tattttaata gttccattat agtccttttt agtactaaat cggaacgaga aattatcttc
     1981 tcccctgttt ttgggtgtga gtgctggctc gacgtgtggg ggcgaaaccc cacatagcgt
     2041 tgatctgtat ggaaaaagaa tcatgctttg aacgtaagaa ttggcggttc ggctggtaca
     2101 attgctagtc caagtcgagc aacttaaccc cgtttttggt ctgttcggct tggcgaaagc
     2161 taaaaaccag ccgaaaaaag ttccgcaagg aagaaacgta gtgaaaaagg tggagatttt
     2221 tgagtgcttt ttactcaaaa atacaacctg ttccttgcgg aacttttagc cctctatata
     2281 tagagtgaga agagagagag ttttaaaagt aaaaaaaacc ttgatatatc agtatttaca
     2341 aagcttttcg tgtgtcacga aatcatgaca catgtgtcat gaaatcatga cacacgaaaa
     2401 gctaaaaaaa ataggattga cgaattgtca atcctaaaat aatttatctg gcaaatcttt
     2461 taatattttc attttcatag ccttataaaa cattacttga agtgcttcat tcacactatc
     2521 tttatctcca gcgtaaataa tgtgtggatt tacaaagacc gcatatgctt tagcattatt
     2581 tccctcaaca cctgaatccc ctttgaataa aagacccttt ttaactaatg aatttattgc
     2641 taagcttgta ttttgtcttg aaatgccaat taattttgca atctctgaaa cattagcagg
     2701 aacaggattt ttagctttaa tatcatgaac aatacaattg ctcgagaaag ctatataagg
     2761 tgttatatca cttaaaaata ctttttcttt attagttaaa tatccatttt tatttaagaa
     2821 acctagattt tcttgcaatg attgagcaaa cctcactctc gaatgtgggc tttttttagt
     2881 acctatgtaa agctctttac cagttgcttt tgccatagtt tccattgctt tttctaattc
     2941 atctttagaa acttcatgat gatcttgctt aaatttttca ttttctattt ctaaatcacg
     3001 tattcttgca tttttctcaa cctgtttaaa ttcaatagtg tttggcatac ttttctcctc
     3061 gtttctagta tttacaacta gcgaagagac ttctattttg ttattatgta gttagataaa
     3121 ttaatttgag tctgaacact ctaattaatt gtataacagt aaaaaaagcc cttcttctat
     3181 agttgtttct gaacaactca attatagcat gaatggcttt ttttgttgta tcttgtctga
     3241 ctttttatat tttcaaaatt aaatatatac atttttttct aagtttgata tcctaaaaat
     3301 gtcaataaga cgaatacctt attacaacca ttagcaaaaa atccttattt tataaggctt
     3361 ttttgctttt taacatcttt ttcaacttga tatttcagaa tattctaaaa tatatttttc
     3421 aaatttaata aattagatat atgttaagac atgataaaat aagtggaaat aatatacatt
     3481 cgtaaaaaag ggggctattt tgtggacgtt caaacaaaaa gtaatgaaca aatcacaaac
     3541 atgttaaatg actggtatat cgaaattcgt gctagacatt taggtaatgc gcataaatta
     3601 agattagaaa ttgataaaaa aatccataac atagaagaag accaaaatct acttttatac
     3661 tattcactat tagattttcg ccaccaatat ctaatcgatc atttaagtat tggtaaggat
     3721 agcttcgaca aaattgaatc ttttaacaca cctacagata atcttctctc atactattat
     3781 ttctttttta aagccataca cgcaactagc atcggaaact acaacttagc aagagaatac
     3841 tatgacaaag ctgaaataaa attaaaagaa ataccagacc aattggaaca cgctgaattt
     3901 tattataaac tttccacatt tagctgccac aatcaacaat atgttccggc tattaaacaa
     3961 gcatctaaag caaaagaaat cttttctaaa tacaaaggat atgaattaaa cataggctat
     4021 tgtaataacc tttctggatt atcatgcaca catctaaaag aatacgaatt agctgaagaa
     4081 tactttatat cagcaataga tatattccaa aaagaaaaag aagaacaagc tattttatat
     4141 gttcgtcata atctaggatt tatgtatgca aaacaaaatt tatcagaatt agcaattcgc
     4201 tatttatccg aagttattca aaaatcacca aataactaca gagcaatctt aattgaagca
     4261 tgcgaacatg caaaactaaa taacaaaaac aaagcattag aacttctcga aaaaggtcta
     4321 aaaattagta ataacctcaa aattgaagaa tatcaacatc gatttaagat tttactagca
     4381 ataaacaatg aaatacctgg agaaaaatta gaacaaataa tattagcagg aatgatctat
     4441 tttgaaaaag aaaatctata tgagtacatt gccgaatata acgaaacatt agcaataaaa
     4501 ttttatcatg aagataatca ctcaaaagca agtaaatatt tctatttaag ttcaaaagca
     4561 agaaaaaaat ctcataataa gggggcttta aaatgaaaaa aacaatccta acattaatgg
     4621 gaattattac tgtctttact ttaactctaa gcaatatcaa tactccaaaa gaaaacaaag
     4681 acccatctat tcaaaaaata atgctaatgt ctgatggaaa cacaggcggt taaccaaata
     4741 aaagaaataa aagccacttt aaagtggctt ttatttcttt tatttaagtt gaaagttcta
     4801 tatttttttt gattttattg catttattat aaggagaata agactcaata caattaaagg
     4861 taaaaacact aaaaccttta aatctgtaaa tccatttgta ttaatctcac ttatcaccaa
     4921 aaataataaa gcaaaaaaca caatgataac taaaaaatac atactaaatt tactgttaaa
     4981 ttttttcccg ataactttat aaatcaatcc aattataaaa agtaggaata tagctatacc
     5041 aatctgtatc attacgaaag tcattatctc aactcctact taaaaaatac ttatctttta
     5101 ttagcgtaaa tttctttatc caaccaacta atcgtcatta ctgaaccacg tgaacttgtg
     5161 tgatcgcgta attctctata acaatgataa tttccaacag acataattac tgaaactgct
     5221 gaagctatgg ctgctggtgg gaaaaatgga gttaataatc cagtaatagc ggctatactt
     5281 ccccaataaa atgacccatc agcaaaatta ttttgccatt gttgggattc actctttgac
     5341 atataccatt taacgcccca ccaataagaa tctacagatg aatattgaat tccatcttta
     5401 gcattaaacc ccactaaatt attcatagaa ctatttgcta atggagtagc ttccagttta
     5461 ttattttcta catttatcgt tgcatatcct gcttcagttg cctgattaat agcttgaata
     5521 cctctttgat aatgctcata aacaaaagga ctaacctgtt ctttaacttt tgaatctaat
     5581 tctaattgat tatgttcatt tggtttaata tatttactaa caatagctac agaatcaata
     5641 atttctttag attgtttttc agtatattga gttcccatcc cttctacaga tttaaaatat
     5701 ttttttgttg tctcttgaat ttgatttttc tcagatacag ttgtattttc ttctgcatga
     5761 gaaacaaccg gtgctaccac tggaaataat aaagccgctg aaagtaatcc tgctcctaat
     5821 tttttcattt tcataatatt catcccttca tatttaattt ccaaataatt aacatttaca
     5881 tatctgaatt tgtaaattat cccattatct ggtacatcat tatattaact tatacttttc
     5941 agaaaataaa caaatttaag aataatacga attaatatat ttcgacattt cctcaaaata
     6001 ctcttacaat ccaataaaat taatagattg aaatcaatct tatttaaatg acactgatcc
     6061 tattcaacaa aaaataaaaa agtgcaaaat ctatctacag atttcgcacc aaaaacagat
     6121 acaatcacta gaaacatagg agagtgtttc actactcatt atagcccaaa tccttacatt
     6181 tttgtgttct gatttgaaaa tatgtaaaca ttcatccttt tatttttgaa ttttctttct
     6241 attttagtta tactagacta taaagaaact aatctccttt aggctgtcaa taaatctaca
     6301 acccaaagac accaaaaaaa ccaattcccg tgtaatggaa ttggtttttt tgtgcaattc
     6361 agtcaaacag caacttgaac ccacattctc aaatgtagtc ttgttaactt taacataatt
     6421 tcaattacat aaacgtaaaa aatctcttta tatataaata tacaaagaga ttttttagat
     6481 gtaaccttta aaaagttatt tgtgatggat atttattgta gcatatttaa atttttagta
     6541 cattttaaac agccactaaa tgaccaccaa aggccactgg aaggccacag aacagctata
     6601 aacgaccact agacgaccac agatcataga aaaagcgtac cagctagaaa tctttagaaa
     6661 cccaataggc acagcctatt tcaccacttt ttatacaaag tgggattcgg tttccttatg
     6721 ctctttttta caacatacct ttttctaacg ttccatgtca tatcctttct gtctactttt
     6781 tacttctctt gcatgttcac gttcaaattg attatctaca tcctttatat ccaattcgtt
     6841 cttaataagt cctctaaacg ccttaaattg ctctttaaag
//
