LOCUS       NC_020379              15185 bp    DNA     circular CON 07-APR-2024
DEFINITION  Bacillus thuringiensis serovar thuringiensis str. IS5056 plasmid
            pIS56-15, complete sequence.
ACCESSION   NC_020379
VERSION     NC_020379.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02604143
            Assembly: GCF_000341665.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis serovar thuringiensis str. IS5056
  ORGANISM  Bacillus thuringiensis serovar thuringiensis str. IS5056
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 15185)
  AUTHORS   Murawska,E., Fiedoruk,K., Bideshi,D.K. and Swiecicka,I.
  TITLE     Complete genome sequence of Bacillus thuringiensis subsp.
            thuringiensis strain IS5056, an isolate highly toxic to Trichoplusia
            ni
  JOURNAL   Genome Announc 1 (2), e0010813 (2013)
   PUBMED   23516221
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15185)
  AUTHORS   Murawska,E., Fiedoruk,K., Bideshi,D.K. and Swiecicka,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2013) Department of Microbiology, University of
            Bialystok, 20B Swierkowa Street, Bialystok PL 15-950, Poland
COMMENT     ##Assembly-Data-START##
            Assembly Method :: GS Data Analysis Software v. 2.8
            Sequencing Technology             :: 454; Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000341665.1-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 03:47:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,892
            CDSs (total)                      :: 6,762
            Genes (coding)                    :: 6,518
            CDSs (with protein)               :: 6,518
            Genes (RNA)                       :: 130
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 121 of 244
            Pseudo Genes (incomplete)         :: 144 of 244
            Pseudo Genes (internal stop)      :: 62 of 244
            Pseudo Genes (multiple problems)  :: 66 of 244
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP004128.1.
            Bacteria available from Izabela Swiecicka (izabelas@uwb.edu.pl).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..15185
                     /organism="Bacillus thuringiensis serovar thuringiensis
                     str. IS5056"
                     /mol_type="genomic DNA"
                     /strain="IS5056"
                     /serovar="thuringiensis"
                     /db_xref="taxon:1286404"
                     /plasmid="pIS56-15"
     gene            join(14994..15185,1..1245)
                     /locus_tag="H175_RS28100"
                     /old_locus_tag="H175_15p01"
     CDS             join(14994..15185,1..1245)
                     /locus_tag="H175_RS28100"
                     /old_locus_tag="H175_15p01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053955.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS231A family transposase"
                     /protein_id="WP_001053955.1"
                     /translation="MNLSIQDELQLFSEELCRHLTPSFLEELAKKLGFVKRKRKFSGSE
                     LATICIWISQRTASDSLVRLCSQLHAATGTLMSPEGLNKRFDKKAVEFLKYIFSILWKG
                     KLCKTSAISSTALTHFQRIRILDATIFQIPKHLASIYPGSGGCAQTAGIKIQLEYDLHS
                     GQFLNFQVGPGKNNDKTFGTECLDTLRPGDLCIRDLGYFSLEDLDQMDQRGAYYISRLK
                     LNHTVYIKNPSPEYFRNGTVKKQSQYIQVDLEHIMNHLKPGQTYEIKEAYIGKNQKLFT
                     RVIIYRLTEKQIQERRKKQAYTESKKGITFSEKSKRLTGINIYVSNTPEGIVPMEQIHD
                     FYSLRWQIEIIFKTWKSLFQIHHWQNIKQERLECHVYGRLIAIFICSSTMFKIRKLLLQ
                     KNKRELSEYKAIGMIQDHVSLLYQAIQRNTQDLTKILIRLFDLLQKNGRKSHRYEKKTI
                     FDIMGVVYEYNGFGKQKKAA"
                     /name="IS4-like element IS231A family transposase"
     gene            complement(1439..4402)
                     /locus_tag="H175_RS28105"
                     /old_locus_tag="H175_15p02"
     CDS             complement(1439..4402)
                     /locus_tag="H175_RS28105"
                     /old_locus_tag="H175_15p02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538378.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_000538378.1"
                     /translation="MGVKQLLSEAQRNELMDLSRLTEWDLVTFHTFSKHDLHLILKHRR
                     GYNRLGFALQLVLIRYPGWSLTEYKDIPQYVVAYVASQLQIPPEEFLVYAKRGNTLWEH
                     LGEIRTEYGYQNFSSEYKETLLQFLVQQAMDNNNTLYLIEITISTLRKMKVILPAMYVI
                     EDIVWEAKQQADQKVYSILHDGLVQEQKDQLDALLLPTINGKSPLAWLKDVPAQPSPES
                     FLKVIDRLQFVQKIGLTIDTTKINTNRLRQLARLGSKYEPYAFRRFNEVKRYSMLVSFL
                     LEITQDLIDYAIEIHDRLMMNLQTKGKKEQDEIQQANGKKLNEKILQFITVCGTLIEAK
                     ETGKDAFAALDEVMSWNEMVESVEEAKQLSRPLNYDYLDLLNTRYSYVRRYAPTLLRSL
                     HFRATKSGEPVLQALDTIHELNETGKRKVPHGAPLHFVSNRWQKHVYDDDGNINRHYYE
                     LAALTELRNHIRSGDIFVSGSRHHKAFDDYLIPYDEWNEVSNIPNGLTAPLKAEDYITD
                     RINRLNEHLEWLSKNSEKLEGVDISQGKLHVERLDRGTPEEAKAFSKLLHSMLPRIKLT
                     DLLIEVASWTGFHDQFIHASTNQSPDQEEQNIVLATLMAMGTNIGLTKMAEATPGISYR
                     QMANASQWRMYDDAMVRAQSILVNFQKEQKLSSYWGDGTTSSSDGMRLSIAVRSLHADS
                     NPHYGTGKGGTIYRFVSDQLSAYHVKVITTNARDALHVLDGLLHHETDLKIEEHYTDTA
                     GYTDQVFALTHLLGFRFAPRIRDLADTKLFSIPGGEEYENVQALLKGKINVNLIKENYE
                     DIRRLAYSVQTGKVSSALIMGKLGSYARQNKLATALGEMGRIEKTLFTLDYISNKAVRR
                     RVQKGLNKGEAINALARIIFFGQRGEFRERALQDQLQRASALNIIINAISVWNTVYMEK
                     AVEELKARGEFREDLMPYAWPLGWEHINFLGEYKFEGLHDTGQMNLRPLRIKEPFYS"
                     /name="Tn3 family transposase"
     gene            complement(4421..5275)
                     /gene="tnpI"
                     /locus_tag="H175_RS28110"
                     /old_locus_tag="H175_15p03"
     CDS             complement(4421..5275)
                     /gene="tnpI"
                     /locus_tag="H175_RS28110"
                     /old_locus_tag="H175_15p03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnP I resolvase"
                     /protein_id="WP_000382147.1"
                     /translation="MDVAKQFSSYLKQENKTENTVQGYTSGIRQYIKWFEGSYDRKLTK
                     LYRQNILEYISYLKNVKMLNAKSINHKISSLAKFNEFLIQKGSQQDQVILKTDMIKVQT
                     VYASPTQIVELDVKKFLQSVLEDNNKRNYAIATLLAYTGVRISEALSIKMNDFNLQTGE
                     CIIRSGKGGKQRIVLLNSKVLSAIKDYLIDRKTYSTAHESPYLFISKKREKLDRTVVNR
                     IFKSYSNVITPHQLRHFFCTNAIEKGFSIHEVANQAGHSNIHTTLLYTNPNQLQLKNKM
                     ELL"
                     /name="tnpI"
     gene            5727..6440
                     /locus_tag="H175_RS28115"
                     /old_locus_tag="H175_15p04"
     CDS             5727..6440
                     /locus_tag="H175_RS28115"
                     /old_locus_tag="H175_15p04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345487.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_000345487.1"
                     /translation="MDFGRFSGQVSKELDVNGNTLRVWCLELESAGYKFERNNRQQRIY
                     YEHDITILKEMKVLMADGTRTLQEAISLLLESSLSKDTDNALTHSINDSDGSEITPFQR
                     NNSAVGVAYAILEQTNEKVAELIKQQQTIALQNQEILNVIHKDNEEKQNVIQAVFEKQQ
                     QIIEQQQDIHLQNQDITKMYLDEREEKLKERQEKNELKEQLSDMQEKMNEMLEFVHQQN
                     EERSKSFFSKFFSKK"
                     /name="MerR family transcriptional regulator"
     gene            <6464..6610
                     /locus_tag="H175_RS35680"
                     /pseudo=""
     CDS             <6464..6610
                     /locus_tag="H175_RS35680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847620.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /name="DUF3967 domain-containing protein"
     gene            complement(6570..7334)
                     /locus_tag="H175_RS28120"
                     /old_locus_tag="H175_15p05"
     CDS             complement(6570..7334)
                     /locus_tag="H175_RS28120"
                     /old_locus_tag="H175_15p05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000900639.1"
                     /translation="MLGILFTAAPSTDVVKDLQDKVISLQDHEVAFLNTTISNMLTAVG
                     IGVAIITAVFTAAFAYVTYSNKRAKKNMDEASRKLEEAESKVSVLEEKSAQLERKILEA
                     EQLLADANSISNVANEKLIEVEEEQKKLRVETRRLNTSAQFDLALDNHKTEIERIKNNL
                     SSLQKFNYPFFSERRLELGTKCFDLESDFRRIKTLLTKEMLIGGDVAKYANEISTFAVE
                     ASSLFDEYKALEDDMAIAPVKSDEKEKEESSK"
                     /name="hypothetical protein"
     gene            7768..8064
                     /locus_tag="H175_RS28125"
                     /old_locus_tag="H175_15p06"
     CDS             7768..8064
                     /locus_tag="H175_RS28125"
                     /old_locus_tag="H175_15p06"
                     /inference="COORDINATES: protein motif:HMM:NF037917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015406534.1"
                     /translation="MFPGFIKHVKKQHPGIYEKYSSHIKDIVEHPDYVGQNPKEPNSVE
                     LVKILNDHILIAIKLDPSGYLFLSTMFDLKNGPAKIQRRLNSGRLIAYKDLLS"
                     /name="hypothetical protein"
     gene            complement(8479..8655)
                     /locus_tag="H175_RS37410"
                     /old_locus_tag="H175_15p07"
     CDS             complement(8479..8655)
                     /locus_tag="H175_RS37410"
                     /old_locus_tag="H175_15p07"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003319293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_003319293.1"
                     /translation="MRKEMAREQRQRFIDLLLDFMVKNPHLFVNGTEDESNNVVTKCNS
                     DIKEVAESYLTLL"
                     /name="hypothetical protein"
     gene            complement(9984..11024)
                     /locus_tag="H175_RS28130"
                     /old_locus_tag="H175_15p09"
     CDS             complement(9984..11024)
                     /locus_tag="H175_RS28130"
                     /old_locus_tag="H175_15p09"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000931944.1"
                     /translation="MLNIEKTVHYYNYAQQYLMMPKAVEEYLTIHDVSLGACKLYMHLR
                     DRYRLSLKSVSEGNDKYEDELGVFCIFPIEDLVEIMGKNEKTVRRWYKELKECDLIQYR
                     QVKKESGQANRFYIKEEQLVDSGLIALYKSEEYKPVVHYPQIGAVKNVRSTSGQKCPPI
                     NNSYLINNKIYSEFLSNSHEFLTTEEAEKIVRKFKGDLDQEPEDNKHFLKFANACYERL
                     CNHDVDPHETWKEHNEKQGAGYVGNQDIPEDTYNQFVDALNDNEGKSITDSKKSHIEGN
                     KKPYLIVNNVPKLTGKQFDKLIVLVRGANSGDPVAWKKCESPEMQSYVEKTDNRFKIRN
                     AVIRGM"
                     /name="hypothetical protein"
     gene            complement(11502..11633)
                     /locus_tag="H175_RS38160"
                     /old_locus_tag="H175_15p10"
     CDS             complement(11502..11633)
                     /locus_tag="H175_RS38160"
                     /old_locus_tag="H175_15p10"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_265340257.1"
                     /translation="MNEMLEEANPNEGETATLDNVNIDHLLECVQLMHVVTKQLQNR"
                     /name="hypothetical protein"
     gene            complement(11772..11978)
                     /locus_tag="H175_RS28135"
                     /old_locus_tag="H175_15p11"
     CDS             complement(11772..11978)
                     /locus_tag="H175_RS28135"
                     /old_locus_tag="H175_15p11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015406536.1"
                     /translation="MSEKKSKFETFYTNKMDYYGVDNIKDAEKAYKMERMIAKNIVEFG
                     IDKVEEWIELSKITGVKDRIKEN"
                     /name="hypothetical protein"
     gene            complement(12621..12989)
                     /locus_tag="H175_RS28140"
                     /old_locus_tag="H175_15p12"
     CDS             complement(12621..12989)
                     /locus_tag="H175_RS28140"
                     /old_locus_tag="H175_15p12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000179528.1"
                     /translation="MATAKKKETTKEPINIDIKIATPKQALATRKLIMDMICRREIEQS
                     DSRQLIYALQGASVEHRSMQEGEKLKIEREKVAIQERAVKVSEEMKLKLEKDNEQLEEE
                     VSELREILKERDERGQRY"
                     /name="hypothetical protein"
     gene            complement(13307..13780)
                     /locus_tag="H175_RS28150"
                     /old_locus_tag="H175_15p14"
     CDS             complement(13307..13780)
                     /locus_tag="H175_RS28150"
                     /old_locus_tag="H175_15p14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000030292.1"
                     /translation="MSKAKRLLREVRRMKASMVPNTAVFIVSDSEQIKDIDPNKRAKEN
                     IFIVSNVPQECINCTKHFSECKCDDSSYRPPTENEYAYWKEKSPRHYERFMRLRDQDEQ
                     HEKTYGLDKWIEDNVLYVRKKKVNGATDKIDQIPLSQMSDEMREVLCKDVKRI"
                     /name="hypothetical protein"
     gene            complement(13788..14039)
                     /locus_tag="H175_RS28155"
                     /old_locus_tag="H175_15p15"
     CDS             complement(13788..14039)
                     /locus_tag="H175_RS28155"
                     /old_locus_tag="H175_15p15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000221334.1"
                     /translation="MTYKIQTDNGYIQLQVTGELTGEAYKPFFDTMADNIEDACKRRIQ
                     EGERVSIRSYKMKKRVSAKGTNAGNNRKAWMKEKLKGE"
                     /name="hypothetical protein"
     gene            complement(14106..14774)
                     /locus_tag="H175_RS28160"
                     /old_locus_tag="H175_15p16"
     CDS             complement(14106..14774)
                     /locus_tag="H175_RS28160"
                     /old_locus_tag="H175_15p16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000172519.1"
                     /translation="MTLIDQMKKEIAELKGSIQRDKLQAINDKIAKYEAKEKGDDLAKR
                     EREKKAQAEAMARAFDGGLNYEDVATGNIYTKVTETVSPELASLYKERDELTALLNEST
                     ADVLGDLNEHEQATHDIANKQVERTAVANGSRTVDLTLVQAPPVDYMALSMDMEIIPGA
                     NPRDLFEKQAGMSFERYTQIATPEEVQKANVFVRPNATKQAQHEQNLQSGAYNNGRIEF
                     "
                     /name="hypothetical protein"
     Typing          complement(9985..11024)
                     /name="rep_cluster_899"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttatgtagcc aattacacgc agctacaggt actcttatga gtccagaagg acttaataaa
       61 cgctttgata aaaaagctgt tgaatttctg aaatatattt tttctatatt atggaaaggt
      121 aaactttgta aaacatcggc tatttcaagc acggcgctta cccactttca acgtattcgt
      181 attttagatg cgacgatttt tcaaatccca aaacatttag ctagtatata tcctggatcc
      241 ggtggttgcg cacaaacagc gggtatcaag attcaattag agtatgattt acatagcggt
      301 caatttttaa actttcaggt aggtccaggg aaaaataatg ataagacctt tggaacggaa
      361 tgtttagata ccttacgtcc aggtgacttg tgcattcgtg atttaggtta tttttcatta
      421 gaagacttag atcaaatgga tcaacgaggt gcctactata tatccagatt aaaattaaac
      481 catactgttt atataaaaaa tccgtctcca gaatactttc gaaacggaac cgtaaaaaag
      541 caatcacagt atattcaagt tgatttagaa catatcatga accatttaaa acccggtcaa
      601 acatatgaaa taaaagaagc ttacattgga aagaatcaaa aactattcac gcgtgtaatt
      661 atctatcgtt taacagaaaa acagatacag gaacgtagaa aaaaacaagc ctatacggaa
      721 agtaaaaagg gcattacgtt ttcagaaaag agtaaacgat taacgggtat caacatatat
      781 gtctcaaata cgcctgaagg gattgttccg atggaacaaa tacatgactt ttactccctt
      841 cgctggcaaa tagaaatcat ttttaaaact tggaaatcct tatttcaaat tcatcattgg
      901 caaaatatca aacaagagcg attagaatgt catgtgtatg gaaggcttat tgccattttt
      961 atctgttctt ctacgatgtt taagatacgt aaacttctgt tgcaaaagaa caaaagagaa
     1021 ttaagtgaat ataaagctat tgggatgatt caagatcatg tatcgctgtt atatcaagcg
     1081 atacagagaa acacccaaga tctaacaaag attttaatcc gtttgttcga cctcctacag
     1141 aagaatggac ggaaatctca tcgatatgag aagaaaacta tctttgatat tatgggtgtt
     1201 gtctatgagt ataatgggtt tggaaaacaa aagaaagctg cataattttt aaaatgaaac
     1261 tcgttagagt ttatttggtg tgtgtacttt tcaataccaa aagtacatta cgagtttttt
     1321 agttgctttg aacgaaagtt cattttattt ttgttcttaa gttgatgggc atggggtacc
     1381 gccagcattt cggaaaaaaa acacgctaag gattttttct ataaaaagag ccgttatatt
     1441 aagaataaaa cggctctttt atacgtaaag gacgtaaatt catttgccca gtgtcatgta
     1501 atccttcaaa tttgtattct ccaagaaaat tgatatgttc ccatcctaac ggccacgcat
     1561 atggcattaa atcttctcta aattctcctc ttgcttttaa ttcttctacg gctttttcca
     1621 tatatacagt gttccacaca cttatagcgt taataattat gtttagtgca ctagctcttt
     1681 gtaactggtc ttggagagca cgttctctaa attctccacg ttgtccaaaa aatataattc
     1741 tagctaatgc attgattgct tctcctttat ttaaaccttt ttgaacccgt ctccttacgg
     1801 ctttattaga tatgtaatcc agcgtaaaga gggttttctc gattcgtccc atttctccaa
     1861 gtgctgttgc gagtttattt tgtcttgcat atgatccgag cttccccatg ataagagcgc
     1921 tagatacttt tcctgtttga accgagtatg ccaaccttct aatatcttca tagttttctt
     1981 ttattaaatt tacattaatt tttcctttta aaagtgcttg aacattttca tattcttctc
     2041 caccaggtat agaaaaaagc ttagtatctg ccaaatcacg aatacgagga gcaaatcgaa
     2101 atcccaacaa gtgtgttaaa gcgaaaactt gatctgtata tccagctgta tctgtgtaat
     2161 gttcttcaat ttttaaatct gtttcatgat gaagaagccc atctaataca tgaagagcat
     2221 ctcgtgcatt ggtagtaatg actttcacat gataggcaga tagctgatca cttacaaatc
     2281 gataaatggt tccacctttt cccgttccat agtgtggatt ggaatctgca tgtagagaac
     2341 gtacagcaat ggataaacgc ataccatcag aagaagaagt tgtcccatct ccccaatagg
     2401 atgataactt ttgttccttt tggaaattca cgagaataga ttgagcgcga accattgcat
     2461 catcatacat tctccattgt gaagcatttg ccatttgtct ataggagatt ccaggggtag
     2521 cttccgccat ttttgtaaga cctatatttg tccccattgc catcaaagta gctaatacaa
     2581 tattctgttc ctcttgatca ggagattgat tggtagaggc gtggataaat tgatcatgaa
     2641 atccagtcca actggccact tctattagta aatctgtcag ttttattctg ggtaacatac
     2701 tatgaagaag tttactaaat gcttttgctt cttctggagt tcctctatcc aatcgctcaa
     2761 catgtagttt tccttgacta atatctactc cttctaattt ctcgctattt tttgataacc
     2821 attctaaatg ttcatttaat cggtttattc ggtcagttat ataatcttca gcttttaaag
     2881 gggctgtaag gccatttgga atgtttgaaa cttcgttcca ttcatcatac ggtataagat
     2941 aatcatcaaa tgccttatga tgtctactgc ctgatacaaa aatatctcct gaacgaatat
     3001 gatttcttaa ctcggttaaa gctgccaatt cataatagtg ccgattaatg tttccgtcat
     3061 catcatatac atgtttttgc caacggttag atacaaagtg taagggagcg ccatgaggca
     3121 cttttcgttt acctgtttca ttcaattcat gaatcgtatc aagtgcttgt aaaacgggtt
     3181 ctccagattt cgtcgctcta aaatgaagac tgcgtaataa agtaggtgca tatcgtctaa
     3241 cataggaata acgagtattt aacaaatcca aatagtcata atttaaaggc cgtgatagtt
     3301 gttttgcttc ttcaacagat tcgaccatct cattccatga cattacttca tctagggctg
     3361 caaaagcatc tttacccgtt tcttttgcct caattagtgt cccgcataca gtaataaatt
     3421 gtaaaatctt ctcgtttaat tttttcccat ttgcttgctg tatttcgtct tgttcttttt
     3481 tcccttttgt ttgaagattc atcataagac gatcatgaat ttcaattgcg taatctataa
     3541 ggtcttgtgt aatctccaat aaaaatgaga ctaacatact atatcttttt acttcattga
     3601 atcgtctaaa agcatatggc tcatattttg atcctaatct agcgagctgc cgaagacgat
     3661 ttgtattaat ttttgtggta tcaatagtaa gaccaatttt ttgaacaaat tgtaatcggt
     3721 ctattacttt taaaaatgat tctggtgaag gctgggccgg tacatctttt agccatgcta
     3781 atggagactt accatttata gttggtaaaa gtaacgcatc taattgatct ttttgttctt
     3841 ggaccaaacc gtcatgtagt atactataca ctttctgatc tgcttgttgc tttgcttccc
     3901 agacgatatc ttcaatcaca tacatagctg gaagaatgac tttcattttt cgcaatgtag
     3961 atattgttat ttcaattaaa tagagggtat tattgttatc cattgcttgt tgcactaaaa
     4021 attgtaataa agtttcttta tattcagaag aaaaattctg atatccatat tctgttcgaa
     4081 tttccccaag atgctcccat aaagtattcc cacgtttggc atatactaaa aattcttcag
     4141 gtggaatctg taactgactc gcaacgtaag ccactacata ttgtggaatg tctttatatt
     4201 ctgttaagga ccagccagga taacgaatta atacaagctg aagagcgaac cctaaacgat
     4261 tgtatcctct acggtgtttt aatattaaat gtaagtcatg tttagaaaaa gtatgaaatg
     4321 ttaccaaatc ccattctgtt aaacgtgata aatccattaa ctcatttcgt tgtgcctctg
     4381 acagtaattg tttaactccc attgtttccc ctttactcct ttataagagc tccattttat
     4441 tttttagctg cagttggttt ggatttgtgt aaagtagtgt cgtatggatg ttagagtgcc
     4501 cagcttgatt tgcaacttca tgaatgctaa atcctttttc aattgcattc gtacagaaga
     4561 agtgtcgtaa ttggtgtgga gtaataacat tgctgtatga tttaaagata cgattgacga
     4621 ccgtacggtc gagcttttct cgctttttac taataaaaag atacggagat tcatgtgctg
     4681 tactgtatgt ttttcgatcg atgagataat ctttgatagc actaagtacc ttactattta
     4741 gtaatacaat tcgttgttta cctccttttc cacttcgaat aatacattcc ccagtctgta
     4801 aattgaagtc attcattttg atagataatg cctctgaaat acgtactcct gtatatgcta
     4861 ggagagtggc aattgcataa ttacgtttgt tattatcctc taacacactt tgtaaaaact
     4921 tttttacatc taattcaaca atttgggttg gagaagcata gacagtttga acctttatca
     4981 tgtctgtttt taaaattact tgatcttgtt gacttccttt ctgtattaga aattcattaa
     5041 atttagcaag gctactaatc ttgtggttaa tggacttggc gttcaacatt ttgacattct
     5101 ttaaataact aatgtactct aagatatttt gtcggtacaa ttttgttaat tttctgtcat
     5161 aggaaccttc aaaccatttt atgtactgtc taatacctga tgtgtatccc tgaacagtgt
     5221 tctcagtttt attctcttgt ttaagataag aagaaaactg ttttgcaaca tccatgtgat
     5281 ttcctccgcg tctataaatt gtgttgcgtc taaaagtatt tatttatcga taaattgtgt
     5341 tgcgttaaac ccctagattt atatttatta taacacctaa tacaacacaa ttaatattgt
     5401 gttgtattat tttttgtata aaaaaagaca ctgtatgtgt cttggaataa tgatatattt
     5461 tctgattttt taactctgat tttcttagcg tggttttttt ccgaaatgct ggcggtaccc
     5521 cttatagtag taaccctttg tccggatggg aattcgtttc tcattttccg actgaatgtg
     5581 aatcttcacc aatgaggtgt cctcagagtt aaggagactt aatagaagta gcaaacggca
     5641 ttagtgactt cgcattaacg gtgttataat ggtgctacaa tgtattaata atgtcattat
     5701 gatgcaagag aataggaggg attccaatgg acttcgggag attttcggga caagttagca
     5761 aagaattaga tgtgaacgga aatacattac gtgtttggtg tttggagtta gaaagcgctg
     5821 gatataaatt tgaacggaat aatcgtcaac aacgcatcta ttacgaacat gacattacta
     5881 tccttaaaga gatgaaggtt ttaatggcgg acggaactag aaccttacaa gaagctatat
     5941 ctctattgct agaatcatct ctttctaaag ataccgacaa tgcactaacg catagcatta
     6001 acgatagtga tggtagtgaa ataacaccgt ttcaacgcaa taacagtgct gtcggagttg
     6061 cttatgctat attggaacaa accaatgaaa aagtggctga attgataaaa cagcaacaaa
     6121 ctatcgccct acaaaaccaa gagattttaa atgttatcca taaagacaac gaggaaaagc
     6181 aaaacgttat tcaagctgtc tttgaaaagc aacaacaaat catcgaacag caacaagata
     6241 tacatttaca gaatcaggac ataacaaaaa tgtatttaga tgaacgtgaa gagaaattga
     6301 aggaaagaca agaaaaaaac gaactgaaag aacagctctc tgacatgcaa gaaaaaatga
     6361 atgaaatgtt agagttcgtt catcaacaaa atgaagagag aagtaaatca ttcttcagta
     6421 agttcttctc taaaaaataa ctataaagcg cctcctgaat catgaaatgc ttatgcaaac
     6481 gatccgtgag gttcaagagg ttaaaaagat gttggctgca agtaaagata gaaagtggta
     6541 cgaattttgg aaaaagagaa agaggagagt cattttgaac tctcctcttt ctctttttca
     6601 tcacttttaa caggtgctat agccatatca tcttctaagg ccttatactc atcaaataag
     6661 ctacttgcct caaccgcaaa tgttgatatt tcattagcgt attttgctac atctccacct
     6721 attaacattt cttttgtaag aagcgttttt attctacgaa aatctgattc taaatcaaaa
     6781 cattttgtac ccaattccaa cctacgttcc gagaaaaatg gataattgaa tttctgtaaa
     6841 ctagaaagat tgttttttat tctttctatc tcagttttat ggttatcaag agctaaatca
     6901 aattgagcac tagtatttaa acgtcttgtt tcaactctta atttcttttg ttcctcttct
     6961 acctctatta acttctcatt ggccacatta gaaatagaat tggcatcagc taagagttgt
     7021 tcagcttcaa gaatcttcct ctccaattga gcgcttttct cctctagcac agaaacttta
     7081 ctttctgctt cttctaattt tctactagcc tcgtccatat tctttttagc acgcttatta
     7141 gaatatgtaa cataagcaaa cgctgctgta aaaaccgccg ttataattgc cactccaata
     7201 cctactgctg ttaacatatt agatatcgtg gtgttcaaaa acgctacctc atgatcctgc
     7261 aatgatataa ctttatcttg caaatccttt acgacatcag tagatggcgc cgctgtaaat
     7321 agtatcccca gcatcaatag ttccccctga ttatataaat tcagacgaat actattatcc
     7381 attatttttt attaaggtaa attaatattt tacctattaa aggagggacg atttaagtat
     7441 ggacgtgatg tttggtcaag gtgggaacat aaattttaat tacattggaa catattgtgg
     7501 acaagcatct aattcataat ccccactttc caagcatgaa agtcccttat accatcaatt
     7561 attcaataaa atatccctat cataaacctg tcccgcgcga cggttcaaat actcctttat
     7621 gataaaataa agatataaag agacgagatg agggagaaat ttgacggcta ataaaaaaag
     7681 attgtaaata aaccaactgt tgtggcaaaa cttccacaaa tcattataga tacatataac
     7741 ttgacttgca cttctaacga tgtaaaaatg tttcccggat ttataaagca cgtaaagaaa
     7801 caacatcctg gtatttatga aaaatattca tcgcacataa aagatatcgt tgaacacccc
     7861 gactatgtcg ggcaaaatcc taaagaacct aatagcgttg aattggtaaa gattttaaat
     7921 gaccatatac taattgctat aaagttggac ccaagcggtt atttgttctt atcaactatg
     7981 ttcgatctaa aaaacggtcc tgcgaaaatc caaagacgat tgaatagtgg acgtttaatt
     8041 gcttacaaag acctgttaag ctaacgggga gtttactaaa atataagaac atggtatact
     8101 acctgtagaa agtaacataa taatgatata ggatctttga ggtgggaaag gttccccacg
     8161 cgcttatcta accgccgttc tcaagcattt gattggctag gtaagttacc agagatgtag
     8221 gatacgccgc cctaccaacg atcctatgta cggtaagcca tccgtttttt acggatggct
     8281 ttttatattg actttttcat cgctaatggt tacttttcta tcaaacgaca ccgaaaggcg
     8341 atgattattg tgttaaaaaa cccaatatta taacatccac taatggataa tcttactttt
     8401 agtaagtagg ttgcctagtg aaatacaata attccaacat tgggccttaa tacatattaa
     8461 ttagttttaa ccctctcact ataaaagagt taaatatgac tccgcaacct ctttaatatc
     8521 actattacat tttgtaacaa cattattact ttcatcctct gtaccattaa caaataaatg
     8581 aggattcttt accataaagt ctaacaatag atcaataaac cgctgccttt gttctctagc
     8641 catttccttt ctcatagaga accactcccc tatctttcac tattctttta atttcatctt
     8701 gtctttcact tgatttaaac agctctggat tcttcctcaa tcccccacta tcttagttaa
     8761 tggtagttgc ttcagtactc tacatttttt gctaatcgag gttaaaatcc ttcaaatccc
     8821 ttgactttaa ggtcttaagg ttttcatact ggcgagtttg ttaggaaatc tgaatgtagt
     8881 tttgggcata gtaacccctg ttgaaaattt acgaggttaa aacgcccact cattcgtact
     8941 ggcttagatg ccgaggtctc gaattgagat tggttgcatt gacgacaaac ggttatagct
     9001 tacaaaacta ctataccgtc cgtatatagc ttattggcat tgtattgcgc cgtacggtgc
     9061 gtttctcacg cccaacaccg ttccctttcg ggtgattcgc tatataaccc gcatatcctt
     9121 gtaataaagc ttgtacaaga atagccgtga atcagtgtca tgatcgccgc ttacgagtaa
     9181 ctgtttaact ccagtcgcac aatcatgtct tattttgaac ccccaacact ttttgccgct
     9241 aacgtcttgg ttatcatcat tattaggatt tacatcccgt aggcttgttt tatgcaaggc
     9301 ttgtgtcctt tacctcgctt acgtcagaca gttgaatgca acgacaactg atatccgagt
     9361 gtactctcct gcattggcta cactagacca tctttttaaa gtggtagctt ccactggaac
     9421 ggaacacaaa aagggcgttc tctatatcta aacgccctgt acatttacaa gacttctagg
     9481 tatagaaaac gcccttttat atccgtttta ttatgtatat gacaatgcta gtaattacta
     9541 gttgaaatat tcgtagagta acggtacaat aggtgtatct aataagcctt gttcgcgaaa
     9601 acaaggcaaa taacttattt gaggttggcg cctcttataa gtcgaagtcc tgtatggctg
     9661 tacaggatta agtcattctc gctaaaggtt ggtagccaat agcatatgag agtggctttt
     9721 ttcatttccg ttctattaag ttactgctaa ttttaccgct atgtttctat taaatcaacg
     9781 aaaatttgat aaggttcacc agaaacatta atatttgcga cataaaactc actctccaac
     9841 tttggaggat ggccaggtat gtgaactttt tcgcacccca tatatagagg gtacgcttaa
     9901 aattaagaac accctcttaa acctctcatt tccatttcta acgctttcta tatcataaaa
     9961 atgtatttat attactttaa cagttacatg cccctaataa ccgcgtttct aatcttaaaa
    10021 cggttgtctg tcttttctac ataactttgc atttctggtg attcacattt tttccacgct
    10081 acaggatcgc cgctattcgc ccctctcact aaaactatta atttatcaaa ttgtttacct
    10141 gttaatttag ggacgttatt gactattaaa taaggcttct tatttccctc tatatgcgat
    10201 ttcttactat cagtaatact tttcccctca ttatcattta aagcatccac aaattgattg
    10261 taagtatcct caggtatatc ctggttacca acgtaccctg caccttgttt ttcattatgt
    10321 tctttccacg tttcgtgtgg atcaacgtca tgattacaca atctttcata acacgcatta
    10381 gcgaatttta agaagtgctt attgtcttca ggttcttggt caagatcccc tttaaacttt
    10441 ctgactatct tttcagcttc ttctgtagtc aaaaactcat gcgagtttga aagaaactca
    10501 gaatatattt tgttattaat taaataactg ttattaatag gtggacattt ttgaccactc
    10561 gtggaccgga catttttgac cgctcctatt tgaggataat gtacgactgg tttgtattct
    10621 tcacttttat ataatgcgat caacccagaa tcgactaatt gttcttcttt aatatagaaa
    10681 cggttagctt gtccactttc ctttttgact tgtctgtatt gaataagatc acattctttt
    10741 aactctttat accatcgtct aacggttttt tcgttcttgc ccatgatctc aactaaatcc
    10801 tctataggaa aaatacagaa tacacctaac tcgtcctcgt atttatcgtt accttcacta
    10861 acactcttta atgatagtct gtaacgatcc ctgaggtgca tatacaactt gcaagcccct
    10921 aaactaacat cgtgaatagt aagatactcc tcaaccgctt tcggcatcat aaggtactgt
    10981 tgagcgtagt tatagtagtg tactgtcttt tcgatattta acataaaaac ctccgattat
    11041 tgtcatttaa gtacgacaac taaacagaga tttcacttgt tcctatacta tgaaattgct
    11101 ataatataca tagtgagtaa ttactgcaat taatcagtga gtaagggaga aatccctggt
    11161 atgcatcaac ccgttgtatc ctaactttgg tcggtgaagg atatagcggg tttttagctg
    11221 tctataccct attatactat gaatctacta aacaaaaaaa ggttctcaga ggaactcttt
    11281 ttgttgatac cgcacttttt cgtataccct taatatttaa ttgaacaagg taaagagacg
    11341 agctcaaaat tgagcccatc tctcactgag gaaacctcaa ttttgaggtt tcctctgcca
    11401 atatacttaa tactggactt gcgaatgcta acatgaacca cattgctgtt gtgaatgaca
    11461 tttgcaaact cctttctagc tcactatgcc agtagtgggc tttatctatt ttgtaattgt
    11521 ttggttacaa cgtgcatcaa ttgaacacac tctaaaaggt gatcgatgtt cacgttatcc
    11581 aacgtcgcgg tttcaccttc atttggatta gcttcttcca gcatttcatt caatccgttt
    11641 accaaccaaa aaacttgctc tctagtaatt gttttttgtc attattcatc ttgaatacct
    11701 cctaattttg gtttcgaaac cattttaata aaaaagagat gggccattgc cctttagttt
    11761 acttctcttt tttagttttc tttaatccga tctttaactc cagtgatttt ggatagttca
    11821 atccactctt ctactttatc aattccaaat tctacaatgt ttttcgcaat catacgctcc
    11881 atcttatatg ctttttcagc atctttaatg ttgtctacac cgtaatagtc catcttattt
    11941 gtatagaatg tctcgaactt actcttcttt tcagacactt gttcatctct cctttacagt
    12001 gaatagtata taacggtttc gaaaccttgt aaaggtagtt tcgaaacttt tttgatgttg
    12061 tttaatagtt tcactttttt cgattgaaaa ggattgataa gaaataagtc tgttgatcag
    12121 aatcagctat ccaattacct tttaatccaa aaagaaaagg catacccaat taggataagc
    12181 cctttcaact gactttgtag aaaacgatac aaaatatatg atccatcgtg acgaacgata
    12241 acacattata acgcttctaa cgttcgggaa taacgatcat tttttccagt tatcttacaa
    12301 taatcactat atcgccatac aatgaccatc taaaaatgac tgtaaaaacg ttcgtgtttt
    12361 ctcgaataga cataaacctt gatataacag cattcttgct actaatatag tttacataat
    12421 acttattata accctaactt ttagtaagtt aataatatac ttatagttag agtgttcaat
    12481 ataagtatta tagcacctgg gccgtgatct gttataacat attttatata cttatatagg
    12541 gtaggggggg ttgcgtgtca tgtgtgcgcg aatcttctcg cgttcagaat taaaaaccct
    12601 ggataaacac ccgataaaac ttagtacctt tgccctcttt catctcgttc ttttagaatc
    12661 tctcttaact cggatacttc ttcttctaat tgctcattat ctttttctaa ttttagcttc
    12721 atctcttctg atactttaac ggccctttcc tggatcgcta ccttttcacg ttcaatcttc
    12781 agtttttcac cttcttgcat gcttctatgc tccactgatg caccttgtag agcatatatt
    12841 aactgtctag agtcactctg ttctatctct cttctacata tcatatccat tattaatttt
    12901 cgtgttgcta gggcttgttt tggtgtagcg attttaatat caatattgat cggttctttt
    12961 gttgtttctt ttttctttgc tgttgccatg ttccatctat cctctctata ataaatttct
    13021 tattcgtcgt gctctttctc tcatttgatt gtcgtacaac tcacttttat gcacgatctg
    13081 gcattcactg tttaatacgc aatcatacaa cgagggatca taaaccttct ttctagtaaa
    13141 cagcacattc atatcatttt catgtagctt accagccatg tataacgcct ttatatccgt
    13201 ccacctagtt ttatatagtt gccctggcgt cattcctagt tcgcgctcca gggccgttat
    13261 atcttccttt gatcgtacat aatcattact tgtcatatat gttggtttaa atgcgtttga
    13321 catctttaca taacacctct ctcatttcat cactcatttg gcttaaaggt atctggtcaa
    13381 tcttatctgt agccccgttt actttctttt tccttacata taaaacgtta tcttcaatcc
    13441 atttatctaa accatatgtt ttttcgtgtt gctcgtcttg atctcgtaac ctcataaacc
    13501 tttcataatg gcgcggactt ttctctttcc aatatgcata ttcgttctct gttggtggtc
    13561 tatagcttga gtcgtcacat ttgcactcac taaagtgttt agtacaatta atacactcct
    13621 gaggtacgtt actaacaata aagatattct cttttgctct tttattagga tcgatatctt
    13681 taatctgttc actatcactt actataaata ctgctgtgtt aggaaccatg ctggctttca
    13741 ttcttcttac ttctcttaat aatcgtttag cctttgacac gttcccttca ctctcctttt
    13801 aatttctctt tcatccacgc tttacggtta ttacctgcat tcgttccctt tgcacttact
    13861 ctctttttca tcttgtatga tcgaatagaa acgcgttcgc cctcctgtat acgcctctta
    13921 catgcatctt ctatgttatc agccattgta tcaaaaaagg gcttgtatgc ctctcctgtt
    13981 aactctccag taacctgtaa ttgtatatac ccgttatctg tttggatctt atatgtcaca
    14041 aggattcctc ctttttatta ataaaagggt aagactgaaa tccctaccct tagttgtaac
    14101 gattcttaaa attcgatacg gccattgtta tatgcaccag actgtaaatt ctgttcatgc
    14161 tgggcctgtt tagttgcgtt tggtctcaca aatacatttg ctttctgtac ttcttctggt
    14221 gttgcaattt gtgtgtatcg ttcaaatgac atgcccgctt gtttttcaaa taagtctcgt
    14281 gggtttgctc ctgggataat ctccatatcc attgataggg ccatataatc aactggtggc
    14341 gcctgtacta gtgtaagatc tactgtacgt gatccatttg caaccgctgt gcgctccact
    14401 tgtttgttag caatatcatg tgttgcctgt tcatgttcgt ttagatcgcc tagtacatcg
    14461 gctgttgatt catttaatag cgctgttaac tcgtcacgtt ctttgtatag gcttgctaac
    14521 tctgggctca ctgtttctgt tacctttgta taaatgttcc ctgtcgctac gtcttcatag
    14581 tttaaaccgc cgtcaaatgc gcgagccatt gcttcagcct gggccttttt ttctcgttcg
    14641 cgtttagcca ggtcatcgcc cttctctttt gcttcatact ttgcaatctt gtcattaatg
    14701 gcctgcaatt tatctcgctg aatgctacct tttaactctg caatctcttt tttcatttga
    14761 tcaataagtg tcatatgtat aaccttcttt cattaattgt tgtattaaat tgtctaacaa
    14821 cttgttacag caataacata acacctttta gtgttatttt cactctttac tttatgtcgt
    14881 aactcttata ggggtaccgc cacatgccca tcaacttaag aatctagact acccccaagt
    14941 gtaaaaaaac gttattcttt ctaaaaagct aactagaaag gatgacgttt tttatgaatc
    15001 tttcgattca agatgaatta caactatttt ctgaagagct gtgccgtcat ttaacccctt
    15061 cttttttaga agaactcgca aaaaaattag gttttgtaaa aagaaaacga aaattttcag
    15121 gaagtgaatt agctaccatt tgtatttgga taagtcaaag gacagcgagt gattccctcg
    15181 ttcga
//
