LOCUS       NC_020378               9671 bp    DNA     circular CON 07-APR-2024
DEFINITION  Bacillus thuringiensis serovar thuringiensis str. IS5056 plasmid
            pIS56-9, complete sequence.
ACCESSION   NC_020378
VERSION     NC_020378.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02604143
            Assembly: GCF_000341665.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis serovar thuringiensis str. IS5056
  ORGANISM  Bacillus thuringiensis serovar thuringiensis str. IS5056
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 9671)
  AUTHORS   Murawska,E., Fiedoruk,K., Bideshi,D.K. and Swiecicka,I.
  TITLE     Complete genome sequence of Bacillus thuringiensis subsp.
            thuringiensis strain IS5056, an isolate highly toxic to Trichoplusia
            ni
  JOURNAL   Genome Announc 1 (2), e0010813 (2013)
   PUBMED   23516221
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9671)
  AUTHORS   Murawska,E., Fiedoruk,K., Bideshi,D.K. and Swiecicka,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2013) Department of Microbiology, University of
            Bialystok, 20B Swierkowa Street, Bialystok PL 15-950, Poland
COMMENT     ##Assembly-Data-START##
            Assembly Method :: GS Data Analysis Software v. 2.8
            Sequencing Technology             :: 454; Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000341665.1-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 03:47:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,892
            CDSs (total)                      :: 6,762
            Genes (coding)                    :: 6,518
            CDSs (with protein)               :: 6,518
            Genes (RNA)                       :: 130
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 121 of 244
            Pseudo Genes (incomplete)         :: 144 of 244
            Pseudo Genes (internal stop)      :: 62 of 244
            Pseudo Genes (multiple problems)  :: 66 of 244
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP004126.1.
            Bacteria available from Izabela Swiecicka (izabelas@uwb.edu.pl).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9671
                     /organism="Bacillus thuringiensis serovar thuringiensis
                     str. IS5056"
                     /mol_type="genomic DNA"
                     /strain="IS5056"
                     /serovar="thuringiensis"
                     /db_xref="taxon:1286404"
                     /plasmid="pIS56-9"
     gene            complement(join(8734..9671,1..1201))
                     /locus_tag="H175_RS27970"
     CDS             complement(join(8734..9671,1..1201))
                     /locus_tag="H175_RS27970"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_087965962.1"
                     /translation="MLVSFLLEITQDLIDYAIEIHDRLMMNLQTKGKKEQDEIQQANGK
                     KLNEKILQFITVCGTLIEAKETGKDAFAALDEVMSWNEMVESVEEAKQLSRPLNYDYLD
                     LLNTRYSYVRRYAPTLLRSLHFRATKSGEPVLQALDTIHELNETGKRKVPHGAPLHFVS
                     NRWQKHVYDDDGNINRHYYELAALTELRNHIRSGDIFVSGSRHHKAFDDYLIPYDEWNE
                     VSNIPNGLTAPLKAEDYITDRINRLNEHLEWLSKNSEKLEGVDISQGKLHVERLDRGTP
                     EEAKAFSKLLHSMLPRIKLTDLLIEVASWTGFHDQFIHASTNQSPDQEEQNIVLATLMA
                     MGTNIGLTKMAEATPGISYRQMANASQWRMYDDAMVRAQSILVNFQKEQKLSSYWGDGT
                     TSSSDGMRLSIAVRSLHADSNPHYGTGKGGTIYRFVSDQLSAYHVKVITTNARDALHVL
                     DGLLHHETDLKIEEHYTDTAGYTDQVFALTHLLGFRFAPRIRDLADTKLFSIPGGEEYE
                     NVQALLKGKINVNLIKENYEDIRRLAYSVQTGKVSSALIMGKLGSYARQNKLATALGEM
                     GRIEKTLFTLDYISNKAVRRRVQKGLNKGEAINALARIIFFGQRGEFRERALQDQLQRA
                     SALNIIINAISVWNTVYMEKAVEELKARGEFREDLMPYAWPLGWEHINFLGEYKFEGLH
                     DTGQMNLRPLRIKEPFYS"
                     /name="Tn3 family transposase"
     gene            complement(1305..2024)
                     /locus_tag="H175_RS37865"
                     /old_locus_tag="H175_9p01"
     CDS             complement(1305..2024)
                     /locus_tag="H175_RS37865"
                     /old_locus_tag="H175_9p01"
                     /inference="COORDINATES: protein motif:HMM:NF025079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="WP_015406530.1"
                     /translation="MGVKQLLSEAQRNELMDLSRLTEWDLVTFHTFSKHDLHLILKHRR
                     GYNRLGFALQLVLIRYPGWSLTEYKDIPQYVVAYVASQLQIPPEEFLVYAKRGNTLWEH
                     LGEIRTEYGYQNFSSEYKETLLQFLVQQAMDNNNTLYLIEITISTLRKMKVILPAMYVI
                     EDIVWEAKQQADQKVYSILHDGLVQEQKDQLDALLLPTINGKSPLAWLKDVPAQPSPES
                     FLKDRPITICSKNWSYY"
                     /name="DUF4158 domain-containing protein"
     gene            complement(2043..2897)
                     /gene="tnpI"
                     /locus_tag="H175_RS27975"
                     /old_locus_tag="H175_9p02"
     CDS             complement(2043..2897)
                     /gene="tnpI"
                     /locus_tag="H175_RS27975"
                     /old_locus_tag="H175_9p02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnP I resolvase"
                     /protein_id="WP_000382147.1"
                     /translation="MDVAKQFSSYLKQENKTENTVQGYTSGIRQYIKWFEGSYDRKLTK
                     LYRQNILEYISYLKNVKMLNAKSINHKISSLAKFNEFLIQKGSQQDQVILKTDMIKVQT
                     VYASPTQIVELDVKKFLQSVLEDNNKRNYAIATLLAYTGVRISEALSIKMNDFNLQTGE
                     CIIRSGKGGKQRIVLLNSKVLSAIKDYLIDRKTYSTAHESPYLFISKKREKLDRTVVNR
                     IFKSYSNVITPHQLRHFFCTNAIEKGFSIHEVANQAGHSNIHTTLLYTNPNQLQLKNKM
                     ELL"
                     /name="tnpI"
     gene            complement(3280..3564)
                     /locus_tag="H175_RS27980"
                     /old_locus_tag="H175_9p03"
     CDS             complement(3280..3564)
                     /locus_tag="H175_RS27980"
                     /old_locus_tag="H175_9p03"
                     /inference="COORDINATES: protein motif:HMM:NF022781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="WP_236635925.1"
                     /translation="MMAIFISIVLVVSFVLFNRVQAKKSIYNYIARQGIQESQLKYIDF
                     HKDFKMGGYWLAVYVEGENPDIYYEYSYQDKKVNFQAYFNSEKAIKKKM"
                     /name="DUF3139 domain-containing protein"
     gene            complement(3610..4422)
                     /locus_tag="H175_RS27985"
                     /old_locus_tag="H175_9p04"
     CDS             complement(3610..4422)
                     /locus_tag="H175_RS27985"
                     /old_locus_tag="H175_9p04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000734781.1"
                     /translation="MKKLLISFIAILFFICGFNLKAFAAEEIIDYQSLYNQAIQEGVLD
                     QNSVSYNEWLKQNKEEFMPIYQDGLKQGVFLEPLSYNEWLKLNNYGQAPTGDIELFDDV
                     TPRGSWGGFTLKAGDIFITNATSSAGIVGHAAIANGDNYILHMPGAGQGNQQLSTSNWM
                     QKYTASGKWIKVYRLKDQTLARDVARYADRNFYSTTGSATKNVYLDYGIDTHLYQKNPT
                     YCSKLVFQALYFGSGSRNVMQAVSGIVTPYGLIDTFTSAYRPSLVKTY"
                     /name="hypothetical protein"
     gene            complement(4985..5599)
                     /locus_tag="H175_RS27990"
                     /old_locus_tag="H175_9p05"
     CDS             complement(4985..5599)
                     /locus_tag="H175_RS27990"
                     /old_locus_tag="H175_9p05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185569.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000185569.1"
                     /translation="MTNTIDFKHVEKNARIRDFENEKEKFKQDHNGINQEEVNQAMQVL
                     SKATGGKEIFIGTKRSPQSKVKFAQFIQDNWDYALENAFFTDEEMLFLLRIQRFLQFKS
                     NCIVNDIHSRNALPMSQKQIADRLKTDKSKISRIVNSLVQKGVIVKANGHKPEGVKART
                     YALFINPNIIYSGERDNVETTLKALFMNSKSLFKKFPIALF"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(6811..8148)
                     /gene="mobV"
                     /locus_tag="H175_RS27995"
                     /old_locus_tag="H175_9p06"
     CDS             complement(6811..8148)
                     /gene="mobV"
                     /locus_tag="H175_RS27995"
                     /old_locus_tag="H175_9p06"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031674.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_001031677.1"
                     /translation="MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNY
                     DLLNQQKIRYESTIKQEISERVKRKPRANSVVLSEFVVTASPDYMHSLSLEEQKRYFES
                     SLDFIQKRYGKQNTLYAMVHMDEATPHMHIGVMPITEDNRLSAKDMFTRKELISLQQDF
                     PLEMREKGFDVDRGEGSEKKHLSPQAFKEKQDLEVEVEQLSNVKTHLKTKVVETHNQLQ
                     QTTNYIEKQNETLQKIQQQFLSLDKKIKEKKQEFETFRNQIPDKPVSMSYLREETKTEV
                     TTKLFGKPEITEKKTGNIVVTREQWRDMTEKVNAAVIVKKDYERLQKTDLVKENQSLRE
                     DNKYLEETIKGNNLALKHSYKQNRELEEVNKELHTEIGTLKAHIRDLQMNIKVLYQQTK
                     KVFKEQFKAFRGLIKNELDMKGVDNQFEREHTREIRSRQKGYDMER"
                     /name="mobV"
     Typing          complement(6815..8148)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagttgtccc atctccccaa taggatgata acttttgttc cttttggaaa ttcacgagaa
       61 tagattgagc gcgaaccatt gcatcatcat acattctcca ttgtgaagca tttgccattt
      121 gtctatagga gattccaggg gtagcttccg ccatttttgt aagacctata tttgtcccca
      181 ttgccatcaa agtagctaat acaatattct gttcctcttg atcaggagat tgattggtag
      241 aggcgtggat aaattgatca tgaaatccag tccaactggc cacttctatt agtaaatctg
      301 tcagttttat tctgggtaac atactatgaa gaagtttact aaatgctttt gcttcttctg
      361 gagttcctct atccaatcgc tcaacatgta gttttccttg actaatatct actccttcta
      421 atttctcgct attttttgat aaccattcta aatgttcatt taatcggttt attcggtcag
      481 ttatataatc ttcagctttt aaaggggctg taaggccatt tggaatgttt gaaacttcgt
      541 tccattcatc atacggtata agataatcat caaatgcctt atgatgtcta ctgcctgata
      601 caaaaatatc tcctgaacga atatgatttc ttaactcggt taaagctgcc aattcataat
      661 agtgccgatt aatgtttccg tcatcatcat atacatgttt ttgccaacgg ttagatacaa
      721 agtgtaaggg agcgccatga ggcacttttc gtttacctgt ttcattcaat tcatgaatcg
      781 tatcaagtgc ttgtaaaacg ggttctccag atttcgtcgc tctaaaatga agactgcgta
      841 ataaagtagg tgcatatcgt ctaacatagg aataacgagt atttaacaaa tccaaatagt
      901 cataatttaa aggccgtgat agttgttttg cttcttcaac agattcgacc atctcattcc
      961 atgacattac ttcatctagg gctgcaaaag catctttacc cgtttctttt gcctcaatta
     1021 gtgtcccgca tacagtaata aattgtaaaa tcttctcgtt taattttttc ccatttgctt
     1081 gctgtatttc gtcttgttct tttttccctt ttgtttgaag attcatcata agacgatcat
     1141 gaatttcaat tgcgtaatct ataaggtctt gtgtaatctc caataaaaat gagactaaca
     1201 tactatatct ttttacttca ttgaatcgtc taaaagcata tggctcatat tttgatccta
     1261 atctagcgag ctgccgaaga cgatttgtat taatttttgt ggtatcaata gtaagaccaa
     1321 ttttttgaac aaattgtaat cggtctatct tttaaaaatg attctggtga aggctgggcc
     1381 ggtacatctt ttagccatgc taatggagac ttaccattta tagttggtaa aagtaacgca
     1441 tctaattgat ctttttgttc ttggaccaaa ccgtcatgta gtatactata cactttctga
     1501 tctgcttgtt gctttgcttc ccagacgata tcttcaatca catacatagc tggaagaatg
     1561 actttcattt ttcgcaatgt agatattgtt atttcaatta aatagagggt attattgtta
     1621 tccattgctt gttgcactaa aaattgtaat aaagtttctt tatattcaga agaaaaattc
     1681 tgatatccat attctgttcg aatttcccca agatgctccc ataaagtatt cccacgtttg
     1741 gcatatacta aaaattcttc aggtggaatc tgtaactgac tcgcaacgta agccactaca
     1801 tattgtggaa tgtctttata ttctgttaag gaccagccag gataacgaat taatacaagc
     1861 tgaagagcga accctaaacg attgtatcct ctacggtgtt ttaatattaa atgtaagtca
     1921 tgtttagaaa aagtatgaaa tgttaccaaa tcccattctg ttaaacgtga taaatccatt
     1981 aactcatttc gttgtgcctc tgacagtaat tgtttaactc ccattgtttc ccctttactc
     2041 ctttataaga gctccatttt attttttagc tgcagttggt ttggatttgt gtaaagtagt
     2101 gtcgtatgga tgttagagtg cccagcttga tttgcaactt catgaatgct aaatcctttt
     2161 tcaattgcat tcgtacagaa gaagtgtcgt aattggtgtg gagtaataac attgctgtat
     2221 gatttaaaga tacgattgac gaccgtacgg tcgagctttt ctcgcttttt actaataaaa
     2281 agatacggag attcatgtgc tgtactgtat gtttttcgat cgatgagata atctttgata
     2341 gcactaagta ccttactatt tagtaataca attcgttgtt tacctccttt tccacttcga
     2401 ataatacatt ccccagtctg taaattgaag tcattcattt tgatagataa tgcctctgaa
     2461 atacgtactc ctgtatatgc taggagagtg gcaattgcat aattacgttt gttattatcc
     2521 tctaacacac tttgtaaaaa cttttttaca tctaattcaa caatttgggt tggagaagca
     2581 tagacagttt gaacctttat catgtctgtt tttaaaatta cttgatcttg ttgacttcct
     2641 ttctgtatta gaaattcatt aaatttagca aggctactaa tcttgtggtt aatggacttg
     2701 gcgttcaaca ttttgacatt ctttaaataa ctaatgtact ctaagatatt ttgtcggtac
     2761 aattttgtta attttctgtc ataggaacct tcaaaccatt ttatgtactg tctaatacct
     2821 gatgtgtatc cctgaacagt gttctcagtt ttattctctt gtttaagata agaagaaaac
     2881 tgttttgcaa catccatgtg atttcctccg cgtctataaa ttgtgttgcg tctaaaagta
     2941 tttatttatc gataaattgt gttgcgttaa acccctagat ttatatttat tataacacct
     3001 aatacaacac aattaatatt gtgttgtatt attttttgta taaaaaaaga cactgtatgt
     3061 gtcttggaat aatgatatat tttctgattt ttttaactct gattttctta gcgtggtttt
     3121 tttccgaaat gctggcggta ccccaattag tacttatcta tttagtacta attagtcttt
     3181 gttttatcct gtatttaagt tcttttttat actgtttatt attgcaaagg tggatacttt
     3241 aattttttca tctctatttc agtaagtcca cttccacccc tacatttttt tctttatcgc
     3301 tttttcagaa ttaaagtaag cttgaaaatt aactttttta tcttgataag aatattcata
     3361 ataaatatca ggattttctc cttccacata aacagcaagc caatatccgc ccattttaaa
     3421 gtctttatga aaatcaatat atttcaactg actttcttgt attccttgtc tagcaatgta
     3481 attatatata gattttttcg cctgtactct attaaataaa acaaaactaa ccactaaaac
     3541 tattgatata aagatagcca tcattattaa catctttttt tttcaaattc aatcctccta
     3601 aattatttat taatatgttt ttactaatga cggtctatat gctgaagtaa aagtatcaat
     3661 taaaccataa ggagttacga ttccactaac cgcttgcata acatttcttg aaccagaacc
     3721 aaaatagagt gcttgaaata ctaatttaga acaataagtt ggattttttt gatacagatg
     3781 tgtatcgata ccataatcta aataaacatt tttagttgca cttccagtag tggaataaaa
     3841 atttctatca gcatatctag ctacatctct agcaagtgtt tgatctttta atcgataaac
     3901 cttaatccat tttcctgacg cagtatattt ttgcatccaa ttagatgtag ataattgttg
     3961 atttccttgg ccagcaccag gcatatgtaa aatataatta tcaccattag caatagctgc
     4021 atgtcctact atacctgctg aactagttgc gtttgttata aatatatctc cagcttttaa
     4081 agtaaatcca ccccaacttc ctcttggagt cacgtcatca aaaagttcaa tatctcccgt
     4141 tggtgcttgt ccataattat ttaattttaa ccattcatta taagataacg gttctagaaa
     4201 aacaccttgt tttaaaccat cttgatatat aggcataaat tcttctttat tttgttttaa
     4261 ccattcatta taagatacac tattttgatc taaaacacct tcttgaattg cttgattata
     4321 aagagattga taatcaataa tttcctcagc agcaaacgct ttcaagttaa aaccacaaat
     4381 aaaaaataaa atagcaataa aactaattaa taattttttc ataaattgtc tccctaaata
     4441 attattatat ttcggtaacc ttttataaag gtaacacgat tttcaaattc aaaatgtaaa
     4501 tacttgtgac aaaaaacata caaatattta ttagctaaat aatgtaattc tcccttgttt
     4561 ttgggtgtga gtgctggcta gtcgtgtggg ggctaaaccc cacatagcgt tgatacatat
     4621 ggaaaaagaa tcatgctttg aacggaagaa ttggcggttc ggctggtaca attgctagcc
     4681 caagtcgagc aacttaaccc cgtttttggt ctgttcggct tggcgaaagc taaaaaccag
     4741 ccgaaaaaag tttggcaagg atgaaacgta gtgaaaaagg tggagatttt tgagtgcttt
     4801 ttcctcaaaa atacgacctg ttccttgcgg aacttttagc ccttatatag agagtgagag
     4861 taaaatacct actaaactag ttaaaaagct tattatatca agggtttata gtggttttaa
     4921 tagttgtcag attagacaac tttagttgtc aaattagaca actattaaaa ttaaaaaata
     4981 gcccttaaaa aagggctatt ggaaattttt taaatagtga tttcgaattc ataaataaag
     5041 cttttaaagt tgtttcaaca ttatcacgtt caccgctata aataatatta ggattaataa
     5101 ataatgcgta tgtacgagct ttaacccctt ctggtttatg tccatttgct tttacaataa
     5161 cacctttttg taccagacta tttacaatgc gactaatttt agatttatca gtttttaatc
     5221 tatcagctat ttgtttttga ctcataggaa gtgcatttct agaatgaata tcatttacaa
     5281 tacaattact tttaaattgt aaaaaccttt gaatacgaag taaaaagagc atttcttcgt
     5341 cagtaaaaaa tgcattttct aaagcataat cccaattatc ttgaataaat tgggcaaact
     5401 ttacttttga ttgcggggaa cgtttagtac ctataaatat ttcttttccg ccagtagctt
     5461 tacttaatac ttgcattgct tgatttactt cttcttgatt aattccgtta tgatcttgtt
     5521 taaacttttc tttttcattc tcaaaatcac gtattcttgc atttttctca acatgtttaa
     5581 aatcaatggt atttgtcatg cttttctcct cgtttctagt atttacaact agcgaagaga
     5641 cttttatttt gttattatga agttagataa attaatttga gtctgaacac tctaattaat
     5701 tgtataacag taaaaaaagc ccttctccta tagttgtaga ttcgacaact caattatagc
     5761 atgaatggct ttttttgttg tatcttgtct gactttttat atgttattgt atagcaagtt
     5821 cgttttagtc gtgagctcat tcattttatt tctaaaaaaa cttcataaag tatcatgcac
     5881 tacagactca gagaaagagc actgttcgaa ggtcctcttt ttttgccgta caggttccca
     5941 tttactttaa aatgaataaa ctatcttaaa ccttacttta acaaatattc atcggttcat
     6001 aacttataaa cacaacccta atacacagaa tgttttttaa acgagcgcct tgccataagt
     6061 gctcgtttta ttttgatgat tttctacaaa atgaaatttt tattgagtat ctttaaccat
     6121 catccgtaca aaattcgcat tctaaatgta tactttcttt acgggaatct ctatttttct
     6181 aaaatcctct gaaaacacca taaaaccaat tatttgaaag taaacaaata ttcaaaagtt
     6241 tactttggaa cttacaattg aaatccaaca tacaatgcta tgagcattta caacatagtc
     6301 ttggtcgaca ataaaaaaag tacgaagtct tttaaaagac ttcgtactaa caacagacta
     6361 aagtacagtt catcggaaac tgaaagggta tttcatctat ttattataaa tcattaattc
     6421 ctatttccgg gaattaatta gaaaatatgc aaaatttcat gttttataat tccttataaa
     6481 caacaaacct agtgaatatt tttgtaattt tctttttatt aatgttagac taaattatat
     6541 aagatacaac tccttaaacg cctagatata tctctattcg ttatacgcaa aaaaaccaat
     6601 tctcgtgtaa tagaattggt tttttgtgca attcagtcaa acagcaactt gaacccacat
     6661 tttcaaatgt agtcttatca atcttaacat aattttaatt acataaacgc aaaaaatctc
     6721 tttatatgca cacatataaa gagattttta aatggatgaa acccataaat tagttagtga
     6781 tgaataatta tagtatatca caaaacgttt ctaacgttcc atgtcatatc ctttctgtct
     6841 acttcttatt tctcttgtat gttcacgttc aaattgatta tctacaccct tcatatccag
     6901 ttcgttttta ataagtcctc taaatgcctt aaattgctct ttaaagactt ttttcgtctg
     6961 ttgatataaa acctttatat tcatctgtaa atctcttata tgagccttta aagtgcctat
     7021 ttcagtatgc aattctttat ttacttcttc taattcccga ttttgtttat aagaatgttt
     7081 taaagcaaga ttattcccct ttattgtttc ttctaaatat ttattatctt ctcgtaaact
     7141 ttgattctct tttacaagat ccgtcttctg taaacgttca taatcttttt taacaataac
     7201 ggctgcattc actttttctg tcatatctct ccattgctca cgtgtaacca caatgtttcc
     7261 tgttttcttc tcagtaattt ctggctttcc aaacagtttc gttgttactt ctgtttttgt
     7321 ttcttctcgc aaataagaca ttgacactgg cttatccgga atttgatttc gaaacgtttc
     7381 aaattcctgt ttcttttctt ttattttttt atctaaactc aaaaattgtt gctgaatttt
     7441 ttgtaacgtt tcattctgtt tttcgatgta gtttgtggtt tgttgtaatt gattatgcgt
     7501 ttccactact tttgtcttta aatgcgtttt tacattggat aactgttcca cttctacttc
     7561 taaatcttgt ttttccttaa aggcttgagg cgataaatgt tttttctcag aaccttctcc
     7621 ccgatctaca tcaaaacctt tttcccgcat ttccaacggg aagtcctgtt gcaaagatat
     7681 caattctttt ctcgtaaaca tatcttttgc ggacaaacga ttgtcttctg taattggcat
     7741 cacaccaata tgcatatgtg gtgtcgcttc atccatatga accatcgcat acaacgtatt
     7801 ttgtttgcca taacgctttt gaataaaatc tagtgaactc tcaaaataac gtttctgttc
     7861 ttctaaactt aatgaatgca tgtaatcagg cgaagctgtt actacaaatt ctgaaagcac
     7921 aacagaattt gcccgtggct ttcgttttac tcgctcactg atctcttgtt taattgtgct
     7981 ttcatatcta attttttgtt gattcaacaa atcataattc tgttcacttt tcgaataatc
     8041 gatgtctaaa tttgatttac tttttccttg tctttgatta tgtttttgaa tcccttgcac
     8101 atcagaaatt tgaaatttct gcatgtgaat gattgcaaat ttgttcatgt ttttccctca
     8161 caattgtctt ccgtaagagg actacttttg agcttatcat aacatatcga gagagcgaat
     8221 ccaaagtttt ggtatagccc attataccaa attacatttg gtgtgggctc tgcgagacct
     8281 cgttggcttc gccaaatcac gccacagcgt gataaagacc aggggctttg ccccacaccc
     8341 cacaagggga actcttcccc ttgaccccat cgatccaact ccccaacccc tcaatccttg
     8401 aggggcttcc tcgccggttg gcgtccccaa aacggttcgt tttgaggaac taccaagagg
     8461 atggaaaatc ggttgcacgt gtccattcct taatgctcct ttcatggatc agcataaacc
     8521 gctgaaccat tccagtccgc ttaccattga cacttaccgc ttatttttct tctcccaata
     8581 gtcgttttgc gagttatttc aatcattcat taaatccttt atcaaactct taattctaaa
     8641 ttaattaaaa aataaaaaga ctaattaggg gtaccgccag catttcggaa aaaaaccacg
     8701 ctaaggattt tttctataaa aagagccgtt atattaagaa taaaacggct cttttatacg
     8761 taaaggacgt aaattcattt gcccagtgtc atgtaatcct tcaaatttgt attctccaag
     8821 aaaattgata tgttcccatc ctaacggcca cgcatatggc attaaatctt ctctaaattc
     8881 tcctcttgct tttaattctt ctacggcttt ttccatatat acagtgttcc acacacttat
     8941 agcgttaata attatgttta gtgcactagc tctttgtaac tggtcttgga gagcacgttc
     9001 tctaaattct ccacgttgtc caaaaaatat aattctagct aatgcattga ttgcttctcc
     9061 tttatttaaa cctttttgaa cccgtctcct tacggcttta ttagatatgt aatccagcgt
     9121 aaagagggtt ttctcgattc gtcccatttc tccaagtgct gttgcgagtt tattttgtct
     9181 tgcatatgat ccgagcttcc ccatgataag agcgctagat acttttcctg tttgaaccga
     9241 gtatgccaac cttctaatat cttcatagtt ttcttttatt aaatttacat taatttttcc
     9301 ttttaaaagt gcttgaacat tttcatattc ttctccacca ggtatagaaa aaagcttagt
     9361 atctgccaaa tcacgaatac gaggagcaaa tcgaaatccc aacaagtgtg ttaaagcgaa
     9421 aacttgatct gtatatccag ctgtatctgt gtaatgttct tcaattttta aatctgtttc
     9481 atgatgaaga agcccatcta atacatgaag agcatctcgt gcattggtag taatgacttt
     9541 cacatgatag gcagatagct gatcacttac aaatcgataa atggttccac cttttcccgt
     9601 tccatagtgt ggattggaat ctgcatgtag agaacgtaca gcaatggata aacgcatacc
     9661 atcagaagaa g
//
