LOCUS       NC_020262               4422 bp    DNA     circular CON 07-APR-2024
DEFINITION  Cronobacter sakazakii SP291 plasmid pSP291-3, complete sequence.
ACCESSION   NC_020262
VERSION     NC_020262.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02604116
            Assembly: GCF_000339015.1
KEYWORDS    RefSeq.
SOURCE      Cronobacter sakazakii SP291
  ORGANISM  Cronobacter sakazakii SP291
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Cronobacter.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Power,K.A., Yan,Q., Fox,E.M., Cooney,S. and Fanning,S.
  TITLE     Genome sequence of Cronobacter sakazakii SP291, a persistent
            thermotolerant isolate derived from a factory producing powdered
            infant formula
  JOURNAL   Genome Announc 1 (2), e0008213 (2013)
   PUBMED   23516209
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Power,K.A., Yan,Q., Fox,E., Cooney,S. and Fanning,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2013) School of Public Health, Physiotherapy &
            Population Science, UCD Centre for Food Safety, WHO-Collaborating
            Centre on Cronobacter, Science Centre South, University College
            Dublin, Belfield, Dublin 4, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.6
            Genome Coverage                   :: 16X
            Sequencing Technology             :: Roche 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000339015.1-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 02:43:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,327
            CDSs (total)                      :: 4,213
            Genes (coding)                    :: 4,129
            CDSs (with protein)               :: 4,129
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 14 of 84
            Pseudo Genes (multiple problems)  :: 15 of 84
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP004094.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Cronobacter sakazakii SP291"
                     /mol_type="genomic DNA"
                     /strain="Sp291"
                     /db_xref="taxon:956149"
                     /plasmid="pSP291-3"
     gene            join(4327..4422,1..249)
                     /locus_tag="CSSP291_RS21070"
                     /old_locus_tag="CSSP291_21363"
     CDS             join(4327..4422,1..249)
                     /locus_tag="CSSP291_RS21070"
                     /old_locus_tag="CSSP291_21363"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_015387218.1"
                     /translation="MSEKRSKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPAR
                     TGKLPSISPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGTDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            239..913
                     /locus_tag="CSSP291_RS22775"
                     /old_locus_tag="CSSP291_21368"
     CDS             239..913
                     /locus_tag="CSSP291_RS22775"
                     /old_locus_tag="CSSP291_21368"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_015387219.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQREGASVLQGKPEEVRELID
                     ASPYVKKYTSGVLSFAEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHEDKGRLE
                     LNFLIPNTELLTGRRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSGL
                     PKTKQEAAEAITRGLLTLASSGELKTRQDVTEALERSGFEVVRTTKSSISIADPDGGET
                     SD"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            926..1417
                     /locus_tag="CSSP291_RS22780"
                     /old_locus_tag="CSSP291_21373"
     CDS             926..1417
                     /locus_tag="CSSP291_RS22780"
                     /old_locus_tag="CSSP291_21373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015060400.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1424..1639
                     /locus_tag="CSSP291_RS22785"
                     /old_locus_tag="CSSP291_21378"
     CDS             1424..1639
                     /locus_tag="CSSP291_RS22785"
                     /old_locus_tag="CSSP291_21378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2326..2541)
                     /locus_tag="CSSP291_RS21080"
                     /old_locus_tag="CSSP291_21383"
     CDS             complement(2326..2541)
                     /locus_tag="CSSP291_RS21080"
                     /old_locus_tag="CSSP291_21383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015387220.1"
                     /translation="MGFWETAGKIAKGAANALDEKAHELQATKMRLESKSSEELKRLIK
                     SDGFFGSASNTEKSMAMKILRERGDV"
                     /name="hypothetical protein"
     gene            2875..3336
                     /locus_tag="CSSP291_RS22790"
     CDS             2875..3336
                     /locus_tag="CSSP291_RS22790"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128348982.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FEMPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMVIRHYYVDCHPYYDRLPPEK
                     SDDFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     Typing          4062..4235
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.714"
                     /coverage="100.0"
                     /evalue="1.2e-79"
     Typing          complement(3535..4138)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.033"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2..243
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.593"
                     /coverage="99.0"
                     /evalue="2.21e-43"
     Typing          875..1731
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.552"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggatgcggc agacctgcct ggacgaaaag cctgcgcgca ccggaaagct cccctcaatc
       61 tcgccggcgc tgctgcgcca gcttgccggc atgggcaata acctgaacca gatagcccgc
      121 cgtgttaacg ccggcggcgg caccggccat gacagggtac agattgtggc ggcgctgatg
      181 gccatcgatg ccgggcttga gcggctgcgg cacgccgttc ttgagaaagg aacggacgat
      241 gatcgttaaa tttcatccac gcggtcgcgg cggtggcgcc gggccggtgg actatcttct
      301 gggtaaagac cggcagcgtg aaggtgccag cgtcctgcag gggaagccgg aagaagtccg
      361 ggagctgatt gacgcgtcgc cttacgtgaa gaaatacacc tccggcgtcc tgtcgtttgc
      421 ggaggccgat ctgcctcccg gccagcggga aaaactgatg gccagcttcg agcgggttct
      481 gatgcccgga cttgataaag atcagtacag cattctgtgg gtggagcacg aggacaaggg
      541 gcgtctggag ctgaactttc tgatccccaa taccgagctg ctgaccggca ggcggctcca
      601 gccgtactac gaccgcgccg accggccgcg tatcgatgcc tggcagacca tcgtgaacgg
      661 cagactgggg ctgcacgacc cgaacgcacc ggaaaaccgc cgggcgctgg tcacgccgtc
      721 cggactgccg aaaacgaagc aggaggccgc agaagccatc acgcgcggac tgctgaccct
      781 tgcttcgtcc ggggagctga aaacccgtca ggacgtcact gaggcgctgg aacggtcagg
      841 ttttgaggtg gtgcgcacca cgaaaagcag tatcagcatt gccgacccgg acgggggcga
      901 aacatccgac tgaagggagc catctatgag cagtctttta acgctggcga aggacttaga
      961 gcagcaatcg aaagcgcaga agcagagtac cggcgagatg ctgaaagccg cattcagcga
     1021 gcacgagcag tctgtcagag cggaactgag cgcaagcgcg aggagaatca gcgacgccat
     1081 cctcgcacac gagcagagta tgagcgaggc gatggagaaa aaccggcgga gcgtactgcg
     1141 gactgccggg cggacatggc tcaccatcct gatggtgtca gcgctgctga tcggcacgag
     1201 cgggagcata ctgtggtggc aggggcagca gataaccgac aattacacgc atcttcgtca
     1261 gcaggaagat accctggcga aaatgaccgc ccggacgtgg ggagtgcgct atcaggagag
     1321 cagcgacggc cggaggtttc ttatcctgcc gccggggatg caaaccgaag ccatccctta
     1381 cgacgggacg acatggatcc gcctgaaaca ggagtagcgg aaaatgacag agctggaaaa
     1441 gcagttgctg agcgcattag agcagctaca gcaggactac tcgcaaaggc tggacgaatg
     1501 ggagagcgcc ttcgcggaat ggcggagcat gtgtgggctt atgcaacggg agaacgcggt
     1561 gctgaacgag cgcgtgtcgg acttgagcac gcaggtgctg agtttaagcg agcagctgcg
     1621 ccgcttgtcc gggaactgaa tgccatcgag ctccggcaga gggagaatga gtatcagaaa
     1681 acactggagc tggagcgcca gccgcagaaa acctaccgcg ggccgacact ttaaaccatc
     1741 caggcagctg acaacccgca cgaagagggg ctcttacttc tcccgctaaa taggttcgtg
     1801 atcaaaaagg tcacgactta ttcaggagcg ggaaatgaag ctgttaaatc ttattgaatc
     1861 attgcttaac tgcctgtatc tggcggcgga gctgtgggac tggctggaga agcatttgtc
     1921 ggtttttaag gcgttcgcgc tgattttagt gtgactttac gcgccatgca tctggctgca
     1981 tgactatgca tcagtgtaat atccagcggc tgcgtttctg gtgttttttc gtcatgtctg
     2041 tcgccacttt ctcgcgtctg gcaccggaaa cgcagccaac cccgtttcag cggtgcgggc
     2101 aatgggacgt tatggtaaat aagtgggcgt aaaaagcccg ggttacgggc ttctggacgc
     2161 tatgacttac cacgacattc tctgcaccaa ctttgattcg ttgcaccaga aggaatggtt
     2221 ctgaatccga atagagtttg caactcacct gcagtatgag cggtggttcg cttgcagtta
     2281 gggcatgtat gaggaagatt caccgtgcct accatttttc tatccttaaa cgtctccgcg
     2341 ttctctgaga attttcattg ccatgctttt ttctgtgtta ctggctgagc cgaaaaaacc
     2401 atcagattta atgaggcgct ttagttcttc actgcttttg ctttctaagc gcattttggt
     2461 tgcctggagt tcatgagctt tttcatccaa agcgttagct gcgccttttg caattttacc
     2521 ggccgtttcc caaaatccca tttaaccact ccttaatgct tatccaattg gacttgttgt
     2581 gatccgacga ctccgacatt tagggcaata atcaggcgtg ccataaccag tacgactggt
     2641 ccactcatgc ttacaatcca aacacttagc ccggtagatt gtcggtgcca ttgaatactc
     2701 cattgatttt aaaaatattt gtatagtgca ctaaaattat cggcagtttt tctcggagct
     2761 tgagaggtag ataaccctaa gctgatatgt tgcctttatg atggttttcg tgtgccaagg
     2821 ttagctaggt aagggattta ctgtgggaag tgctggttcg cgaagtgaag cgttatggta
     2881 aatacatatt atggttattt attaggagta actctgggag agtgggctat gggatcagat
     2941 gttcaatggc agatagataa tggttatttg gcgcaagtta tgggggattt ttcgacagag
     3001 tcgcctagct ttgaaatgcc aatgtttggg cgatattttg atacctttca agctgctata
     3061 catgttgcaa aagctttccc agggagtacg ttgcagccat cagagtacga agataaaact
     3121 gggatggtaa tacgtcatta ttatgtggat tgtcatccgt attatgatcg acttcctcct
     3181 gaaaaaagcg atgattttca gtatgatgaa tacccttctg ctattcatgt ggctgcgtgt
     3241 attgatcaag ccagcaaaga actcgacgaa ctattgtttg gtggtggata tggggaaaat
     3301 ggtgaggggc atgcaagccc gataatttta tattagtgtg agggggcgga gggatattat
     3361 ccaatttgta aaatgatctt cttgagatcg ttttgctctg cgcgtaatct cttgctctga
     3421 aaacgaaaaa ccacctgggg aggtggtttt tcgaaggttc agtaagttgg ggaacttctg
     3481 aaccgtggta acagtcttgt gcgagacgtg tcaccaaatc tgtccttcca gtgtagccgt
     3541 agtccggcca ccacttcaag aactctcgat acatctctcg cacatcctgc ttaccagtgg
     3601 ctaccgccaa tggcgatttt gtcgtgtctt tccgggttgg actcaagttg atagttaccg
     3661 gaatgggcgt ggtagtcgga ctgaacgggg gttcgtgcat acagtccagc ttggagcgaa
     3721 ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga
     3781 gtgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga
     3841 aacgcctggt atctttaagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt
     3901 cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg cttcgccgcg gccttctcgt
     3961 ttccctgtta agtctcttcc tcgcatctac caggaaattt ccgccccgct cgtaagccat
     4021 ttccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaata
     4081 tatcctgtac cacatattct gctgacgcac cggtgccgcc ttttttctcc tgccacatga
     4141 agcacttcac tgacaccctc atccgtgcca acatagtaag ccagtataca ctccgctagc
     4201 gcttcgtgac tggttcaggg ctgcgccccg aaacccgcca aaagcctgac gcgcctgcgg
     4261 cttgtccaac cccgcgccga ccgggaaaga ggttcccgcc cgtcccggcg ttatcaggag
     4321 accggtttgt cagagaaacg cagcaaaatg ctcacgatgt gggtgaccga agatgagcac
     4381 cgccgtctgc tggaacgctg cgacggtaaa cagctcgcgg cc
//
