LOCUS       NC_020250              11769 bp    DNA     circular CON 08-APR-2024
DEFINITION  Bacillus thuringiensis serovar kurstaki str. HD73 plasmid pHT11,
            complete sequence.
ACCESSION   NC_020250
VERSION     NC_020250.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02603412
            Assembly: GCF_000338755.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis serovar kurstaki str. HD73
  ORGANISM  Bacillus thuringiensis serovar kurstaki str. HD73
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 11769)
  AUTHORS   Liu,G., Song,L., Shu,C., Wang,P., Deng,C., Peng,Q., Lereclus,D.,
            Wang,X., Huang,D., Zhang,J. and Song,F.
  TITLE     Complete genome sequence of Bacillus thuringiensis subsp. kurstaki
            strain HD73
  JOURNAL   Genome Announc 1 (2), e0008013 (2013)
   PUBMED   23516207
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11769)
  AUTHORS   Liu,G., Song,L., Shu,C., Wang,P., Deng,C., Peng,Q., Lereclus,D.,
            Wang,X., Huang,D., Zhang,J. and Song,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2013) State Key Laboratory for Biology of Plant
            Diseases and Insect Pests, Institute of Plant Protection, Chinese
            Academy of Agricultural Sciences, Yuanmingyuan West Road Haidian
            District, Beijing, Beijing 100094, China
REFERENCE   3  (bases 1 to 11769)
  AUTHORS   Song,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2013) State Key Laboratory for Biology of Plant
            Diseases and Insect Pests, Institute of Plant Protection, Chinese
            Academy of Agricultural Sciences, Yuanmingyuan West Road Haidian
            District, Beijing, Beijing 100094, China
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: newbler v. 2.3
            Sequencing Technology :: Sanger dideoxy sequencing; 454; Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000338755.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 03:12:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,160
            CDSs (total)                      :: 6,015
            Genes (coding)                    :: 5,686
            CDSs (with protein)               :: 5,686
            Genes (RNA)                       :: 145
            rRNAs                             :: 12, 12, 12 (5S, 16S, 23S)
            complete rRNAs                    :: 12, 12, 12 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 329
            CDSs (without protein)            :: 329
            Pseudo Genes (ambiguous residues) :: 0 of 329
            Pseudo Genes (frameshifted)       :: 155 of 329
            Pseudo Genes (incomplete)         :: 186 of 329
            Pseudo Genes (internal stop)      :: 48 of 329
            Pseudo Genes (multiple problems)  :: 50 of 329
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP004073.1.
            The bacteria is available from Fuping Song (fpsong@ippcaas.cn).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11769
                     /organism="Bacillus thuringiensis serovar kurstaki str.
                     HD73"
                     /mol_type="genomic DNA"
                     /strain="HD73"
                     /serovar="kurstaki"
                     /db_xref="taxon:1279365"
                     /plasmid="pHT11"
     gene            593..745
                     /locus_tag="HD73_RS33070"
                     /old_locus_tag="HD73_8005"
     CDS             593..745
                     /locus_tag="HD73_RS33070"
                     /old_locus_tag="HD73_8005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001113828.1"
                     /translation="MPEFGFRPSMSQLFGNFHMNWSTMMPLLVILFGILFGFFVAHLVK
                     DKFDD"
                     /name="hypothetical protein"
     gene            766..924
                     /locus_tag="HD73_RS33075"
                     /old_locus_tag="HD73_8006"
     CDS             766..924
                     /locus_tag="HD73_RS33075"
                     /old_locus_tag="HD73_8006"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425806.1"
                     /translation="MEMPKITLTYNLADVANGLTNWLGAYWPIIAFSVAIPLTFLVAFN
                     TKDLFTR"
                     /name="hypothetical protein"
     gene            1031..2437
                     /locus_tag="HD73_RS30620"
                     /old_locus_tag="HD73_8007"
     CDS             1031..2437
                     /locus_tag="HD73_RS30620"
                     /old_locus_tag="HD73_8007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015386301.1"
                     /translation="MKKLAIIFLCAFCIVSHTIPVFADSPSFQLVGSKNVTIENNGIVN
                     GKYNLIIKVNNMKDVYSFQLSELMLVNDCSFHVGKSKWTRPEQEKNEFPINWNQNGTYA
                     FYIFHNGKVLGYARFKLKGFKENGGGGGETVYWGKGAKYYDLPEIEKPNPEDYADYDNK
                     NDGVCTNEKIPDDKPQGSGDIDESKEDNKPPVDGGGGSDKAVKEVLEKIVSAVNEISKN
                     TGETAKNTGETAKNTGEIKKNTDEIAKNTKEIADAVKDIRDNLKIPDDMDLSIKPVLPE
                     DIKPDKPKQPDKPFEDKQEHFKEGEQEKPPDKLPVAPEPAECWGDVCKDKDNQKEEDMK
                     KEKDMDKEEPMKPDKPMEKEDMKPDKPMTPDKPMEKEDMKPDKPMTPDKPMEKEDMKPD
                     KPMTPDKPMEKEDMKPDKPMTPDKPMDREGEMKKDNDMKQDKPMTPWEEMKPSDEGSEL
                     CWHVVNGKCG"
                     /name="hypothetical protein"
     gene            2404..3831
                     /locus_tag="HD73_RS30625"
                     /old_locus_tag="HD73_8008"
     CDS             2404..3831
                     /locus_tag="HD73_RS30625"
                     /old_locus_tag="HD73_8008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090439.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="WP_228176732.1"
                     /translation="MLACSEWKMRIKKFFIVVPVLLGILFVGDISAFAQGKDVLSGRVG
                     KAQYGSSYITSLTDGNLQTSDTLSNGEVIIDLASPVRVSGFRFTKVGGSSNRMDVYFYT
                     EGATLKREYRNTDSGYLQAIDIKGVKKIGLKNMSNGDYFTLAEVNLYEYESVPPSDITD
                     LKENIKHDSVEFTYKNPKSNFSHLRIYRDGQVLASDVKEEKFTDKGLTPETEHIYKFVS
                     VSPDGLESKGIEKKIKTAKEPLPDEIKDLQEDEITHTYVKFSYKFPKNNFDHVKIYRDD
                     KVIADNVKVEEFVDKGLKGSTVYTYKFVSVSSTGLESNGIVKKVQTDEEPNLNKPSKPS
                     NFDITPKDGSLVVNLLSQNLGVPIKGYHIFIDGVKVNDSLVTSRSYAVKGLQNGQTYGV
                     QIKAVSKWNIESDLSNVVNGIPKVPVIPEVTFSFGLGALVDGITNWFLGIWPIVAFSIA
                     IPLAFIVAFNTKKLFMR"
                     /name="fibronectin type III domain-containing protein"
     gene            3841..3996
                     /locus_tag="HD73_RS33080"
                     /old_locus_tag="HD73_8009"
     CDS             3841..3996
                     /locus_tag="HD73_RS33080"
                     /old_locus_tag="HD73_8009"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015386302.1"
                     /translation="MIQPVTTKDLIQFLQYAFHAGEDVLFLAFVLMITFSVAVSIRRIM
                     VGYKQS"
                     /name="hypothetical protein"
     gene            3993..4418
                     /locus_tag="HD73_RS30630"
                     /old_locus_tag="HD73_8010"
     CDS             3993..4418
                     /locus_tag="HD73_RS30630"
                     /old_locus_tag="HD73_8010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000970219.1"
                     /translation="MMEKFVKFLKGGFEAVIKSLKTFIDFLATPFQYLLDFLKGIFYFI
                     EKLFQVVVAIIKLFVALFQFLGAVIAGFFKTLFLWVQPNFDGGNVSFPSASMKGFKVAT
                     DILLPTGLLTVIPAVVTCFIWYLFVKKLMDLFRGGHH"
                     /name="hypothetical protein"
     gene            4418..4678
                     /locus_tag="HD73_RS30635"
                     /old_locus_tag="HD73_8011"
     CDS             4418..4678
                     /locus_tag="HD73_RS30635"
                     /old_locus_tag="HD73_8011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000992628.1"
                     /translation="MNALIDTIFNPVLGWLAQMAKYLSDLSVPASRPLRISDYLGVFAY
                     LYGWTTVITTLFALVFVYFVCYIIMTYSSLFIRFKDTVKWW"
                     /name="hypothetical protein"
     gene            5099..5461
                     /locus_tag="HD73_RS30640"
                     /old_locus_tag="HD73_8013"
     CDS             5099..5461
                     /locus_tag="HD73_RS30640"
                     /old_locus_tag="HD73_8013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000880242.1"
                     /translation="MLAKTKRKVQPQFEGHRALIIKEEGTCVVEQVISFDSEILETSTA
                     MLPRENELRLLDDNHGYVYHIYNLEKPARIEAENLKMLRRSSALKGIFDFDTGKKPFDI
                     IGFMPWVIIVLLAIFK"
                     /name="hypothetical protein"
     gene            5475..5837
                     /locus_tag="HD73_RS30645"
                     /old_locus_tag="HD73_8014"
     CDS             5475..5837
                     /locus_tag="HD73_RS30645"
                     /old_locus_tag="HD73_8014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000116007.1"
                     /translation="MSVQEMLTKDILEEEGSNETIETVQLFQFMKKHAVPLTKKQQMAM
                     LLLNENGLSDISLYIAGMKEEVLPLSEYHKTLEKLTLSDRIKGNAKLSHLLKANANPSL
                     SLSANSAKVVKGNELA"
                     /name="hypothetical protein"
     gene            5834..6436
                     /locus_tag="HD73_RS30650"
                     /old_locus_tag="HD73_8015"
     CDS             5834..6436
                     /locus_tag="HD73_RS30650"
                     /old_locus_tag="HD73_8015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024420.1"
                     /translation="MNIMVFEGFLGSGKTFGMSLFAKHYEEKSGCVLYSNYGLIGSKPF
                     VTLDTFHDIAKEKSSILNLDEAHIDLDARSFSSNSVKFFSQLSYYLRKLRCTLFITSPS
                     FDDLDSRIRGITNVLVRVSNDKNYFYYKMYDVQSKRYLKTMRIQKKKAFAIGSKVYDTT
                     AMVSPVQVPDKRQDFMEFLEALKSTAEEYGRQYKHSA"
                     /name="hypothetical protein"
     gene            6507..6698
                     /locus_tag="HD73_RS30655"
                     /old_locus_tag="HD73_8016"
     CDS             6507..6698
                     /locus_tag="HD73_RS30655"
                     /old_locus_tag="HD73_8016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000511841.1"
                     /translation="MGILKKKKFREEVKRINKAHGEMREFLDLLMDRYGLDEEEVKNCE
                     VIKHHFDNLDLMFSQMAK"
                     /name="hypothetical protein"
     gene            7316..7522
                     /locus_tag="HD73_RS30660"
                     /old_locus_tag="HD73_8017"
     CDS             7316..7522
                     /locus_tag="HD73_RS30660"
                     /old_locus_tag="HD73_8017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000365510.1"
                     /translation="MDLPVCNEKEVTVLSKTLKYLRSDQSNQVKVHKEYLEDRGWIFDD
                     LTYIDSNIYVVYKKEVVKRNKAF"
                     /name="hypothetical protein"
     gene            7534..7953
                     /locus_tag="HD73_RS30665"
                     /old_locus_tag="HD73_8018"
     CDS             7534..7953
                     /locus_tag="HD73_RS30665"
                     /old_locus_tag="HD73_8018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000473093.1"
                     /translation="MFKKFYREYIHVLFNIVAMFFYFSWCLVGAEFNLLTLIAIGIALF
                     IFVKYVSMDKKFVNENIDKLKDKKMIFRKYAISGEDIGYAVFTNFLLFILYLGISVYFK
                     PLSVMALLFAFIVNVSFLMLTLRIMSRLSLKNSKV"
                     /name="hypothetical protein"
     gene            7965..8300
                     /locus_tag="HD73_RS30670"
                     /old_locus_tag="HD73_8019"
     CDS             7965..8300
                     /locus_tag="HD73_RS30670"
                     /old_locus_tag="HD73_8019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000457469.1"
                     /translation="MFAKYYSQDDEFMSPLQFIALGGIMSFLIKFGCISPSVNMLTLFV
                     TFVTCIGLCYFFLLLITKYFYRFLAKHRDNLHVYLCNKYNEKIANRIGLCIGLVYIVGV
                     LVICLLI"
                     /name="hypothetical protein"
     gene            8599..8811
                     /locus_tag="HD73_RS30680"
                     /old_locus_tag="HD73_8021"
     CDS             8599..8811
                     /locus_tag="HD73_RS30680"
                     /old_locus_tag="HD73_8021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000216293.1"
                     /translation="MTVKEKKKTWAFTFTESEIQIIDEIVDIENDRRHSIAREHNLPFK
                     KYNRSTFVLAMIEEKKRKYQEQGEI"
                     /name="hypothetical protein"
     gene            9014..9235
                     /locus_tag="HD73_RS30685"
                     /old_locus_tag="HD73_8022"
     CDS             9014..9235
                     /locus_tag="HD73_RS30685"
                     /old_locus_tag="HD73_8022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000391857.1"
                     /translation="MEELLHKIIDKLDKIDKDIQEIKTDIKAHRIETDDNFIKLYHLVE
                     EKDIEIDVLNKRLFKNEATVERIKREKH"
                     /name="hypothetical protein"
     gene            9249..9527
                     /locus_tag="HD73_RS30690"
                     /old_locus_tag="HD73_8023"
     CDS             9249..9527
                     /locus_tag="HD73_RS30690"
                     /old_locus_tag="HD73_8023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000331888.1"
                     /translation="MAYSYCVDCERVYNCEDEKCCCGSKKRSVDWEEFAFIMGYPLYPV
                     KKKYGVFHLDLSKRLDADALCDLKADNPELFYYEFKGWKERELKKRN"
                     /name="hypothetical protein"
     gene            9854..10045
                     /locus_tag="HD73_RS30695"
                     /old_locus_tag="HD73_8024"
     CDS             9854..10045
                     /locus_tag="HD73_RS30695"
                     /old_locus_tag="HD73_8024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000048809.1"
                     /translation="MSKVKGIRLNLRVSEDEYNKLQYVKECLLMEGLDLKDSAVIRYAL
                     LQTYKDYKEMNDLLKSRN"
                     /name="hypothetical protein"
     gene            10232..11302
                     /locus_tag="HD73_RS30700"
                     /old_locus_tag="HD73_8025"
     CDS             10232..11302
                     /locus_tag="HD73_RS30700"
                     /old_locus_tag="HD73_8025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410863.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000410863.1"
                     /translation="MEKHIEKLSLSVCVDWLEFTFVYGEEFESICSFLGLDPTVFSKEI
                     DGFHKSYGYLSRYSFEEIHILMHGADNRSRIIMSGSGCRWFETLSSVGWYGLFDRIAFA
                     DEHGFSWIKVDRLDIAIDDFVGYFSVKKLKSKIKRRECLSRWKTSFVVETFDLEQGRLQ
                     GSTIYFGSASSKLRWVVYDKLEEQKKKNKDSDSIQGLEFWTRHELRLKKERADRAVKQL
                     VVNDFSIEEMYFGIINNYISFRVVDKEDSNRNRWNIAPFWRQYIGQASKLRLAEALPVA
                     NVERTFKWLSHSVSKKLYLLSEIFSQDDDTKKEFASYLENKGKQKITKGDLDLIENFPI
                     RDFKEFLKIYNEKCLK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            11463..11768
                     /locus_tag="HD73_RS30705"
                     /old_locus_tag="HD73_8026"
     CDS             11463..11768
                     /locus_tag="HD73_RS30705"
                     /old_locus_tag="HD73_8026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001176491.1"
                     /translation="MQLMKVMLTGCKKVDFTNDNNERIEGVNIHFLTTTDNSEQSGFGY
                     LPSKVFFRKEFIEKFSSLNYPIVAEAIIAFNMSPRGIQTKISDFNILEEFPFTALK"
                     /name="hypothetical protein"
     Typing          10233..11302
                     /name="rep_cluster_814"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          10233..11299
                     /name="MOBT"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttatgatag ataaaatcga tttaaatgaa ttgtacaaaa ctatttcgga aattatgcct
       61 aattacttag tgcagactaa gaatggtagt tttgcagttt tccagacact tacctatggt
      121 gatatgttaa ttagtttctt gttattgcta atcttgttgg tgttgacatt caactggata
      181 tggagtgctt tacgatgatc gagtttaatt tagaaaactt gttgaatcaa tattccttgt
      241 ttggattacc atttttatta attatcgtgt tcacattgct gaaagattgg cggtttgata
      301 gataatgaat gatttagcgt ttctagatat gttgtattgg ttcttcatga acaattcagt
      361 tgttttatac tcgttaggtt ttataacctt tctagtattc gtaataatga tgttcaccac
      421 cgtcaaagct tggggtgatg gctattagtt ttagcatgga cgtgggaaga atcttacagt
      481 atgcctttaa catgtttagt tcggtgttac cagtgattta tttattcatt ggtggggcgt
      541 ttgctatcta ctgtatcggt aaagtgtaca acacctttag gagttgattt caatgcctga
      601 atttggattc agaccatcga tgtctcagtt attcggtaac tttcatatga actggtcaac
      661 aatgatgcca ttgttagtca ttttgtttgg gatactgttt ggatttttcg tagcgcattt
      721 agtaaaagac aaatttgatg attgattaat taggaggaat ttattttgga aatgccaaaa
      781 attacattga cttataatct tgcagacgtt gctaacggtt tgactaactg gttaggagca
      841 tattggccta ttatcgcatt ctcggttgcg attccattaa cgttcttagt ggcgtttaac
      901 actaaagacc tatttactcg ttaatcatca aataatcact ccttaaatag caagaagtta
      961 actataaatt taataaaaca attaaatagc tgggctaata acctagctat ttttgttagg
     1021 aaggattgat atgaaaaagc tagccataat ttttttatgt gcattttgta ttgttagcca
     1081 tacgatccct gtgtttgctg attcacctag ttttcaattg gtaggtagta aaaatgtaac
     1141 aatcgaaaat aatgggatag ttaatggtaa atacaattta attattaaag ttaacaatat
     1201 gaaagatgta tatagttttc agttatcgga attgatgctt gttaatgatt gctcgttcca
     1261 tgttggtaaa tcaaagtgga ctcgtccaga acaagaaaaa aacgagttcc ctattaactg
     1321 gaatcagaat ggtacatacg cattttacat ttttcataat ggtaaagttt taggctatgc
     1381 acgttttaag cttaaaggat ttaaagaaaa cggtggcggt ggaggagaaa ctgtatattg
     1441 gggcaaaggt gctaaatact acgatttacc tgaaattgaa aagcctaatc ctgaggacta
     1501 tgcggattat gataataaga atgacggagt ttgtacaaat gaaaaaattc cagatgataa
     1561 accacaaggc agcggtgata tagatgaatc aaaagaagat aataaaccac ctgtagatgg
     1621 tggcggtggt tctgataaag ctgttaaaga agttttagaa aagattgtta gtgcagtaaa
     1681 tgaaatttca aagaatacag gtgaaacggc taaaaataca ggtgagacag caaagaatac
     1741 aggtgagatt aaaaagaaca ccgatgaaat agcaaagaac acaaaagaaa ttgcggatgc
     1801 tgttaaggat attagggata atttgaagat tcctgacgat atggatttgt cgattaaacc
     1861 tgttctacct gaggacatta agcctgataa acctaaacaa cctgataagc cttttgaaga
     1921 caaacaagag cactttaaag agggtgaaca agagaaacca cctgacaaac ttcctgttgc
     1981 tcctgagccc gctgaatgtt ggggggatgt ttgtaaagat aaagataatc agaaagaaga
     2041 agatatgaag aaagaaaaag atatggataa agaagaacca atgaagcctg ataaaccaat
     2101 ggaaaaggaa gacatgaaac cagataagcc aatgactcct gataagccaa tggaaaagga
     2161 agacatgaag cctgataagc caatgactcc tgataagcca atggaaaagg aagacatgaa
     2221 gcctgataaa ccaatgactc ctgataaacc aatggaaaag gaagacatga aacctgataa
     2281 accaatgact cctgataagc ctatggatcg ggagggagaa atgaaaaaag ataatgatat
     2341 gaaacaagat aagccaatga ctccttggga agaaatgaaa ccaagtgacg aggggagtga
     2401 actttgttgg catgtagtga atggaaaatg cggataaaga aattttttat agtagtacct
     2461 gtattacttg ggattttgtt tgtaggtgat ataagtgcat ttgctcaagg taaagatgta
     2521 ttaagtggaa gagtaggtaa agctcaatat ggtagttcat atataacttc actaactgat
     2581 ggaaatttac aaactagtga tactctttct aatggtgagg tcataattga tttagcttct
     2641 cctgttagag tttcagggtt taggtttact aaagttggtg gctctagtaa taggatggat
     2701 gtttattttt atacagaggg tgctacttta aagcgggaat atagaaatac tgatagtggt
     2761 tatctacaag ccattgatat taaaggtgtt aaaaaaattg gattgaaaaa tatgagcaat
     2821 ggagattatt ttacacttgc tgaagttaat ttatatgagt atgaatctgt tcctccatct
     2881 gatattacgg atttaaaaga aaatattaaa catgattcag ttgaatttac ttataaaaat
     2941 ccgaagtcga attttagtca tttaagaatt tatcgtgatg gacaagtgct tgctagtgat
     3001 gtgaaagaag aaaaattcac tgataaaggt ttaactcctg aaacggagca tatttataaa
     3061 tttgtttcag tttctcctga cggtcttgaa tcaaaaggta tagagaaaaa gataaagaca
     3121 gcgaaagagc ctttaccaga tgaaataaaa gatttacagg aagatgaaat aactcatact
     3181 tatgtgaaat ttagttataa gtttcctaaa aataattttg atcatgtgaa aatttatcgt
     3241 gatgataagg tgattgcgga taatgtgaag gttgaggaat ttgttgataa aggtctaaaa
     3301 ggaagtactg tatatactta taaatttgtt tcagtttcct ctactggtct tgaatcaaat
     3361 ggaattgtga agaaagtaca gactgatgaa gaacctaatc tcaataagcc ttctaaacct
     3421 tcgaattttg atattacgcc aaaagatggc tcgttagttg taaatctatt aagtcaaaat
     3481 ttaggcgttc caattaaggg ttatcacatt tttattgatg gtgtaaaagt taatgattca
     3541 cttgttacaa gtcgttctta tgctgttaaa ggtttacaga atggtcaaac ttacggtgta
     3601 cagattaagg cggtttctaa gtggaatatt gaaagtgatt taagcaatgt tgttaatgga
     3661 attcctaaag taccagttat tcctgaggtt acctttagtt ttggtttagg tgctttagtt
     3721 gatgggataa caaattggtt cctggggatc tggccgattg tggcatttag tattgctatt
     3781 ccattagcgt ttattgttgc gtttaatacg aaaaagttgt ttatgaggta ggtgcttagt
     3841 ttgatacaac cagttacaac taaggactta atacaatttc tccagtatgc gttccatgct
     3901 ggggaagacg tattatttct agcgtttgta ttaatgatta cattctctgt tgctgtaagc
     3961 attagaagga ttatggtggg ttataagcaa tcatgatgga gaagtttgtt aagttcttaa
     4021 aaggtggatt tgaagcggtt ataaagtcgt taaaaacgtt tattgatttt ttagctacac
     4081 catttcaata tctactggat tttttgaaag gaatctttta tttcatagaa aagctgtttc
     4141 aggttgtagt agcaattata aaattattcg ttgccttgtt tcagttttta ggtgctgtaa
     4201 tagcgggatt ctttaaaact ttatttctat gggtacaacc taattttgat ggtgggaatg
     4261 tatcgtttcc tagtgcatca atgaaaggtt ttaaagtagc tactgatata ttacttccca
     4321 ctggattact tacagttatt ccggctgtgg tcacatgttt tatttggtac ttgtttgtta
     4381 aaaagctcat ggatctattc agaggtggtc atcattaatg aatgcgttaa ttgatacaat
     4441 atttaatcct gttcttggtt ggcttgcaca aatggcaaag tatttaagtg atttaagcgt
     4501 accggcaagc cgtcctttgc gaattagtga ttaccttgga gtatttgctt atttatacgg
     4561 ttggacaacc gttataacga cgttattcgc tttagttttc gtatattttg tttgttacat
     4621 catcatgact tacagtagtt tatttattcg atttaaagat acagtgaaat ggtggtaatg
     4681 atattgaata ttgtaggaca agcaatttta ttaatatttt ttacgacagt tgtttcttgg
     4741 atggtaatag atacattgaa tttctttaga ggtatagaag aatgatttat gcaacgttgg
     4801 ggtttattag ttggattttg attttgattt gtgaagtaac atcgagaaaa ggagatttaa
     4861 aataatgcag gcttttaatg cgatgtttga gttatctagt gcggtttgtt tatctgtatt
     4921 tctttttata gttttaccat atatgttttt caaaaataaa atgtcaaagt gataaaaacg
     4981 aatgttaaat atcaattagt ttatatagtt cgttaaaaag attcaaaatg tcatggcagc
     5041 gggactagat ccaagagatt ttgaaatgaa aaagcgaacg ttttggaggg ttttataaat
     5101 gttagctaaa acaaaacgga aagtacagcc acaatttgaa gggcatagag cattgattat
     5161 taaagaagaa ggaacatgtg tagttgaaca ggttatatcg tttgattcgg agattttaga
     5221 aacgagtaca gccatgttac cacgtgagaa tgaattgaga ttacttgatg ataatcatgg
     5281 ttatgtgtat cacatttaca acttagaaaa gccagcaaga attgaagcgg aaaatttgaa
     5341 aatgttacgt cgttctagtg cattaaaggg aatttttgat tttgatacag ggaagaaacc
     5401 ttttgacatt attggtttta tgccttgggt aattattgta ttgctagcta tttttaaata
     5461 atggggtgga gtaaatgagt gttcaggaaa tgttaacgaa agatatttta gaagaagaag
     5521 gttcaaacga aacgattgag actgttcaac tgtttcaatt catgaaaaaa catgctgtgc
     5581 cattaacaaa gaaacaacaa atggctatgt tattgcttaa tgaaaatgga ttatcggaca
     5641 tttctttata tattgctgga atgaaagaag aagtattgcc attgagtgaa taccacaaga
     5701 cacttgaaaa attaacgctt tctgaccgaa ttaaaggtaa tgctaaatta agtcatttac
     5761 tgaaagcaaa tgcgaatcct tcattatcat tgtcagcaaa tagcgcaaaa gttgttaaag
     5821 ggaatgagtt agcatgaaca taatggtttt tgagggcttt ctcggtagcg gtaaaacgtt
     5881 tggtatgagt ttgtttgcaa aacactatga agaaaagagt ggctgtgtat tgtacagtaa
     5941 ttacggttta attgggtcta aaccatttgt aactttagat acgtttcatg atattgcaaa
     6001 agagaaatct agtatcttaa atctcgatga agcgcatatt gatcttgatg ctagaagttt
     6061 ttcaagtaac tcggtaaagt tcttttctca attgtcttat taccttagaa aactacgttg
     6121 tacgttgttt attacctcac catcatttga tgatttagat tcacgtataa gaggtattac
     6181 aaacgttctc gttagggtat cgaatgataa aaactatttt tattacaaaa tgtatgatgt
     6241 tcaatctaaa cgatatttaa aaacaatgcg tattcaaaag aagaaagctt ttgctattgg
     6301 ttcaaaagtt tatgatacga cagcaatggt ttctccagta caagttccag ataaacgaca
     6361 agacttcatg gaatttttag aagcgttgaa gtcgaccgcc gaagagtacg gacgccagta
     6421 caagcattca gcgtaagctg tatgcgcggt gcaggcggga ggacttcata agattatata
     6481 aattaaataa ggaaggtacg ttatagatgg gaattttaaa gaagaaaaaa tttagagaag
     6541 aagtgaaaag aataaataaa gcacatggag aaatgagaga atttctagat ttgctcatgg
     6601 atcgctacgg attagatgaa gaagaagtaa aaaattgtga agtaattaaa catcattttg
     6661 ataatttaga tttgatgttt agtcaaatgg caaaataatg ttcgtgattt aggtgtgaaa
     6721 tgtaacggca gccgtgcata ttttccaggg gcttttaatg aaaatacgaa cattaagtat
     6781 gaggtgaaga gatttgatac atgtagattg gttgaaaact atggcgagag acggattatt
     6841 ttatggttca ttcattgctg taattgtttt cttgctaaat tggtttacgg gttaggtttt
     6901 aaaggtcttc cttcgctttt gaggttggag attggctggg gtgtggggct agccccgcat
     6961 agcgtaatgg gtaaatagaa taaaagaaaa tataaaggtc ggtagtacaa gcgtttaggc
     7021 tcgtataatg gataggcaag ttgatagtta aatatattca cttttgagtt tatcgacttg
     7081 gcgatgaaga caaacgagcc taaaaaagtt atgcaagaat gtagcgtagc ggaaaaggtg
     7141 gagattttat gaagcagagc gtaagaaaat actacctgtt tcttgtttaa ccttttagca
     7201 aatacagata gagggttgtt ttgatggaga atgttattgt tacgttgttt ggtactctag
     7261 ggatttgttt cttgtttaaa ggcatgctag atactttaaa agaagggtga tatttgtgga
     7321 tttgcctgtt tgtaatgaaa aagaggtaac tgttttatct aagacattga aatatttgag
     7381 aagtgatcaa tctaatcaag ttaaggtaca taaagagtat ttagaggaca gaggttggat
     7441 ttttgatgat ttaacttata tagatagcaa tatctatgta gtttataaaa aagaggttgt
     7501 aaagcgaaat aaggcgtttt aaggggtgta tatatgttta aaaagtttta tcgtgaatat
     7561 atccatgtat tgtttaatat tgttgcgatg tttttttatt ttagttggtg tttagttggt
     7621 gctgaattta atttattaac tctaatagca attggaattg ccctttttat ttttgttaag
     7681 tatgtatcta tggataagaa atttgttaat gaaaatatag ataaattaaa agacaaaaag
     7741 atgattttta ggaaatatgc tataagtggt gaagatattg gatatgcagt ttttacaaat
     7801 tttttattgt ttattttgta tttaggaata tccgtatatt ttaagccttt aagtgtaatg
     7861 gctttattat ttgcttttat tgttaatgtt agttttctta tgttaactct tagaataatg
     7921 agtagattaa gtttaaagaa tagtaaagtt taagaggtgt atatatgttt gctaagtatt
     7981 atagccaaga tgatgaattt atgtcgccac ttcaatttat agctttgggt ggtattatga
     8041 gttttcttat taaatttggt tgtatttcgc ctagtgtgaa tatgttaacg ttatttgtta
     8101 ctttcgttac ttgtattgga ttatgttatt tttttctact tttaataaca aagtattttt
     8161 atagatttct tgctaaacat cgtgataatt tacatgttta tttatgtaat aagtacaatg
     8221 aaaaaatagc aaatagaatc ggtttatgta ttggtttagt ttatattgta ggggtgttag
     8281 ttatatgttt gctgatttaa agttgaattt taaattttgg ttaactgtag tttttgatat
     8341 atgtatgctt gcaatgatct gtgttgttac aacaaagtat gtatctgata tatatagtga
     8401 tttgtttatt ttcacgacag gatttttaat actattaatt tggtattctg ttagagtgta
     8461 tttgatagat ttaatcagaa aaaaacctat caataattaa aatttaatat ttttttattg
     8521 aaaaaccata tgtttttatt tacaaaccat atggtttttg ttatattaaa tttagttaat
     8581 tgataggagg ttgtttttat gacagttaaa gaaaagaaaa aaacttgggc atttacattt
     8641 actgaaagtg aaattcagat aatagatgaa attgtagata tagagaatga tagaagacat
     8701 tcaattgcta gagaacataa tttaccgttt aaaaaatata atcgttctac atttgtgctt
     8761 gcaatgattg aagaaaagaa acgtaagtat caagagcaag gagaaattta atggaaattg
     8821 ttttaggtgt tagtgcgttt ggtttgtata tttcgttttt aatattttta tattggatgg
     8881 cattagattt tttagcttgg ttatataaaa agatttaata aaatcgttat tttattggtt
     8941 ttctcttaac ttgttaatat taatagggga tagagtctaa cttttttaaa attatatttt
     9001 ggaggtcgta actatggaag aattgttaca taagattatt gataaattag acaagattga
     9061 taaggatatt caggagatta aaactgatat taaggctcac cgaattgaaa ctgatgataa
     9121 ttttattaag ctatatcatt tagttgaaga aaaagatatt gagattgatg tgttgaataa
     9181 gcgtttgttt aagaacgagg cgacagttga acgtattaag agggaaaagc attaggagga
     9241 aattgtcgat ggcatatagt tattgtgtgg attgtgagcg tgtgtataac tgtgaagatg
     9301 aaaagtgttg ttgtggaagt aaaaaacgtt ctgttgattg ggaagagttt gcttttatta
     9361 tgggttatcc gttatatcct gttaagaaaa aatacggtgt ttttcattta gatttatcta
     9421 aacgtttaga tgctgatgct ctatgtgatc ttaaagcaga taatccagaa cttttttatt
     9481 atgagtttaa aggttggaaa gaaagagaat tgaaaaagag aaattaataa taaatatttt
     9541 ggagttagtg ggtgttttgt ttgtctaatt cgaaggaaaa tttccagttg cgaaatgagg
     9601 aacccccgtc cagtaacacg ggggttcccg ggccatctga tatttacaca atcccttccc
     9661 tggactgggt tcaagtcact ttcaaatctg tacagaattc acaaaaaaat gcacggaaaa
     9721 ttattagtaa aatttccagt ttttaatgaa gcttaaaaga ttcttatatt ggtataatat
     9781 ttacaaggag gaaaatggaa actctttaca tatggtgtat gacattgtat gcttcaattg
     9841 aggaggttat ataatgtcaa aagttaaagg tattcgtttg aatttaagag tttctgaaga
     9901 tgagtataac aagttgcagt atgttaaaga atgtttactt atggaaggtc ttgatcttaa
     9961 agattcagct gttattcgtt atgctttatt acagacttat aaggattata aagaaatgaa
    10021 tgatttgttg aaatcaagga attgaatagc tgatttcatg tttctactgg gggctagtaa
    10081 caccccagta gaaaatacag gtcaatcacc tccgaacgct tgtggctcta gggttcgtcc
    10141 ttctttgttt ttttcacggt tcttttaaaa aataggcaaa aaattcgcgg tttttatttt
    10201 gcttttattg tattgtttta gaggtgtttg tatggaaaaa catattgaaa aattgtctct
    10261 ttcggtttgt gtggactggt tggagtttac ttttgtttat ggtgaagagt ttgaatctat
    10321 ttgtagtttt ttaggtctag atcctactgt tttttctaag gaaatagatg gttttcacaa
    10381 gtcttatggt tatttgtcta gatattcttt tgaagaaata cacattctta tgcatggtgc
    10441 tgataatcgt tctagaatta ttatgtctgg ttctggttgc agatggtttg aaacattatc
    10501 ctctgttggc tggtatggtt tgtttgatag gattgcattt gcagacgaac atggtttttc
    10561 ttggattaaa gttgaccgtt tagatattgc tattgatgat tttgtaggtt atttttctgt
    10621 taaaaaatta aagtctaaaa taaaaagaag agaatgtttg agtcgttgga aaacaagttt
    10681 tgttgttgaa acttttgatt tagaacaggg acgattgcag ggttctacga tttattttgg
    10741 aagtgcttct agtaaattga gatgggttgt ttatgataag ttagaagagc agaaaaaaaa
    10801 gaataaagat agtgattcga ttcaaggttt agaattttgg actaggcatg agttgcgttt
    10861 gaaaaaggag cgtgctgatc gtgctgttaa acaacttgta gttaatgatt ttagtattga
    10921 agaaatgtat tttggtatta taaataatta tatttcgttt cgtgttgtcg ataaggaaga
    10981 ttctaataga aataggtgga atattgcacc tttttggagg caatatatag gacaagcaag
    11041 taaattaaga ttagcagagg ctttacctgt tgcaaatgtt gagcgtacgt ttaaatggtt
    11101 gtctcatagt gtttctaaaa aattatattt gttgtctgaa attttttcgc aagatgatga
    11161 tacaaagaaa gagtttgcta gttatttaga aaataagggc aaacaaaaaa tcacgaaagg
    11221 ggatttagac ttaatagaaa atttcccaat tcgtgacttc aaagaatttt taaagattta
    11281 taatgaaaaa tgtctaaaat aattttactc tattttataa tggaagtcta atacattaaa
    11341 aaatccatat atttttaaaa aaagactaga atttttttct agtttttctt ataattaaat
    11401 gtatgacaaa ggtgtacata gaacgacatt gtatgactaa aaagattcta aggagtgtaa
    11461 atatgcaatt aatgaaagta atgttaacag gttgtaagaa agttgatttc acaaatgata
    11521 ataatgaaag aatcgaaggt gttaatattc actttttaac tacaactgat aatagtgagc
    11581 aatctggttt tggatatctt ccttctaagg tcttttttag aaaagagttt atagaaaaat
    11641 ttagttcttt gaattatcca attgttgctg aagctattat tgcttttaat atgtctcctc
    11701 gtggtattca aactaaaatt tctgatttta atattttaga ggaatttcct tttacagctt
    11761 taaagtgat
//
