LOCUS       NC_020241               8513 bp    DNA     circular CON 08-APR-2024
DEFINITION  Bacillus thuringiensis serovar kurstaki str. HD73 plasmid pHT8_1,
            complete sequence.
ACCESSION   NC_020241
VERSION     NC_020241.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02603412
            Assembly: GCF_000338755.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis serovar kurstaki str. HD73
  ORGANISM  Bacillus thuringiensis serovar kurstaki str. HD73
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Liu,G., Song,L., Shu,C., Wang,P., Deng,C., Peng,Q., Lereclus,D.,
            Wang,X., Huang,D., Zhang,J. and Song,F.
  TITLE     Complete genome sequence of Bacillus thuringiensis subsp. kurstaki
            strain HD73
  JOURNAL   Genome Announc 1 (2), e0008013 (2013)
   PUBMED   23516207
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8513)
  AUTHORS   Liu,G., Song,L., Shu,C., Wang,P., Deng,C., Peng,Q., Lereclus,D.,
            Wang,X., Huang,D., Zhang,J. and Song,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2013) State Key Laboratory for Biology of Plant
            Diseases and Insect Pests, Institute of Plant Protection, Chinese
            Academy of Agricultural Sciences, Yuanmingyuan West Road Haidian
            District, Beijing, Beijing 100094, China
REFERENCE   3  (bases 1 to 8513)
  AUTHORS   Song,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2013) State Key Laboratory for Biology of Plant
            Diseases and Insect Pests, Institute of Plant Protection, Chinese
            Academy of Agricultural Sciences, Yuanmingyuan West Road Haidian
            District, Beijing, Beijing 100094, China
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: newbler v. 2.3
            Sequencing Technology :: Sanger dideoxy sequencing; 454; Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000338755.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 03:12:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,160
            CDSs (total)                      :: 6,015
            Genes (coding)                    :: 5,686
            CDSs (with protein)               :: 5,686
            Genes (RNA)                       :: 145
            rRNAs                             :: 12, 12, 12 (5S, 16S, 23S)
            complete rRNAs                    :: 12, 12, 12 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 329
            CDSs (without protein)            :: 329
            Pseudo Genes (ambiguous residues) :: 0 of 329
            Pseudo Genes (frameshifted)       :: 155 of 329
            Pseudo Genes (incomplete)         :: 186 of 329
            Pseudo Genes (internal stop)      :: 48 of 329
            Pseudo Genes (multiple problems)  :: 50 of 329
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP004074.1.
            The bacteria is available from Fuping Song (fpsong@ippcaas.cn).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="Bacillus thuringiensis serovar kurstaki str.
                     HD73"
                     /mol_type="genomic DNA"
                     /strain="HD73"
                     /serovar="kurstaki"
                     /db_xref="taxon:1279365"
                     /plasmid="pHT8_1"
     gene            complement(330..593)
                     /locus_tag="HD73_RS30715"
                     /old_locus_tag="HD73_8502"
     CDS             complement(330..593)
                     /locus_tag="HD73_RS30715"
                     /old_locus_tag="HD73_8502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874967.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPNELIKIGISINDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            867..1094
                     /locus_tag="HD73_RS30720"
                     /old_locus_tag="HD73_8503"
     CDS             867..1094
                     /locus_tag="HD73_RS30720"
                     /old_locus_tag="HD73_8503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869869.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000869869.1"
                     /translation="MKVVSNIRMIMAKKNIDNISDLVRITGVSRNSINKLWHNENVSSL
                     KLDTLITICEKLDVELLDLIEYIRDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            1566..2645
                     /locus_tag="HD73_RS30725"
                     /old_locus_tag="HD73_8505"
     CDS             1566..2645
                     /locus_tag="HD73_RS30725"
                     /old_locus_tag="HD73_8505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RapH N-terminal domain-containing protein"
                     /protein_id="WP_001102658.1"
                     /translation="MNVQLGNEQITKLLNDWYQEIRAQHIIKAKQLKQKIEKEINNIEE
                     DQNLLLYYSLIDLRYKILTNDYADREQSLEKIEQLKEHTNNFLDYYYHFFKGMHAMKTG
                     NYSETQKYYDIAEKLLEEIPDEVEKAEFNYAVATFYYHTHQALLATQYANKAKSFFSGK
                     LGYEIKTGACENTLGMACITLREFSVAEEYLLSAINKFEKHSENKLALIVRYNLGLLYA
                     DQNLSELAIRYLLESFENQEDDYKTMFLLAQEYYKLKQTNTVNTYIEKGLNVCNQEYKI
                     HFLFLQALNNKLSTEQLEKVMLDAIPYFEKQNLWKYIQDYTEELAIRFYEEKNKDKSNE
                     YFYKSYKAKRNLLERESLK"
                     /name="RapH N-terminal domain-containing protein"
     gene            2642..2788
                     /locus_tag="HD73_RS33570"
                     /old_locus_tag="HD73_8506"
     CDS             2642..2788
                     /locus_tag="HD73_RS33570"
                     /old_locus_tag="HD73_8506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000976019.1"
                     /translation="MMKKFSLILIGVACTTGIFFSQFNNSIQTHDAKEKNDIIQQYAHG
                     KDI"
                     /name="hypothetical protein"
     gene            2908..3489
                     /locus_tag="HD73_RS30730"
                     /old_locus_tag="HD73_8507"
     CDS             2908..3489
                     /locus_tag="HD73_RS30730"
                     /old_locus_tag="HD73_8507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000723966.1"
                     /translation="MKKISIERSNWRKTYKKMIPVLLVGGILAGNVAFVTNNVFAATTN
                     NNNIIQTKQSLVSGQDEKSINELAEAFQFMHEEAAIYNNQGEIVDFDFNKIEAKYGNDV
                     GLDALKQQIKKDKSDIAGLSFTSCMKDALYDFFGVNAVQAAFNGGLAYYLEKKLWKEAA
                     KIGVKFFIGSSVAGLAATLAYYGGKCAIYG"
                     /name="hypothetical protein"
     gene            3482..3679
                     /locus_tag="HD73_RS30735"
                     /old_locus_tag="HD73_8508"
     CDS             3482..3679
                     /locus_tag="HD73_RS30735"
                     /old_locus_tag="HD73_8508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000361625.1"
                     /translation="MDKSSILKQMYFILFPVTLFLSLNIYQWIVKSDYSFTGYIIQNLI
                     IVALALVAAIIAYLITKKNI"
                     /name="hypothetical protein"
     gene            5052..6257
                     /gene="mobV"
                     /locus_tag="HD73_RS30745"
                     /old_locus_tag="HD73_8513"
     CDS             5052..6257
                     /gene="mobV"
                     /locus_tag="HD73_RS30745"
                     /old_locus_tag="HD73_8513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000033305.1"
                     /translation="MSKFVICHMQKFKMRDVKGLQIHNQREKESHSNSDIIQARTEQNY
                     DLIHDKEKVDYKKLIQNRIDNGVVSNRAIRKDAVVCCSFMISASPDYMNSLSQVEQRRF
                     FEESLRFLKGRYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSEFHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVTLQQEIEELEQEKKGIDSRLHDLKM
                     SLDQAKSVDEIPVKEKGGFIRSKTVEITSEDFESIKVLARSSEALREDVRRLKNESGVI
                     TREKDGLYREKCILEGQITELKRENRGLKEENDFFKKTLERVKELYKEKLPELAGVIGY
                     VKGSILDRMNCKFLKRYFTSDDEVRGAQKFLNHKQEHEEQQKRLKQVRSSQQKNRDQGL
                     ER"
                     /name="mobV"
     gene            6727..7773
                     /locus_tag="HD73_RS30750"
                     /old_locus_tag="HD73_8515"
     CDS             6727..7773
                     /locus_tag="HD73_RS30750"
                     /old_locus_tag="HD73_8515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085408.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001085408.1"
                     /translation="MAKKNQDEITGKLQPKKKQNIKIYEFIKKKMSESGKELFKSCSIF
                     NEFLATKDKKKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADMVKSEIDRFNKAFKKLFDRRNIQRSIKGYIRKLEMTYDKERFITEEMYNN
                     KKRKAYYDSRGLKVDDHNPNYDTYNPHFHVLLCVDKNYFKRKELYIKQEEWLEMWREVT
                     DMPEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQEVFDVFYLALKGRQLIVYGG
                     LLKDYAKKYEDGELDKYKSTDKNEYYYRLIAMWNKDLMKFEQEYQELSESEKQEYNGHL
                     INEVDVEA"
                     /name="protein rep"
     gene            7777..7974
                     /locus_tag="HD73_RS30755"
                     /old_locus_tag="HD73_8516"
     CDS             7777..7974
                     /locus_tag="HD73_RS30755"
                     /old_locus_tag="HD73_8516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000861196.1"
                     /translation="MKVAGDLYYYCLGCKKFHEYEKIDHKGVNRKLCFYCFKIQSKKTK
                     IIGDAEGRMQICETCHKELF"
                     /name="hypothetical protein"
     Typing          6703..7755
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.934"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          5053..6254
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctcgcatat ttaattcata gatatttcat atcgttaaaa agttgcaccg tttcaaacga
       61 gttgtgtgat aaaaagatgt ttagattatg tttgaaattg ttgtttctgt gaatttttta
      121 tttcgcgatt tctgtatatt tttaatcctt tttactctta aaatcttttt gcttttgtgc
      181 gcaaatgaaa aaatcaaaaa gtcacgcgta acttttatta aatatataaa tatattatta
      241 aaatacctaa cccttttatg atgaaaaaat ataaatagtt aaagaaaaaa gactgataaa
      301 aaatatcagt cttttttctt tagttttatt tataaagagt atctctttat ggtgttttct
      361 actttttctc tccagttctc tatatcgtta attgatattc cgatctttat taattcgttt
      421 ggaatgaaac tgttatctaa aatccataaa gtgagccttt ctaatgtgat tctttcgttt
      481 ccaattgaat aaatacgttc actttgtttg caagtatatg gttttttacc aacatatttt
      541 tctacataaa agtatctttt tccgtttgct ttgtaatttg ttaggaatgc caaaatgata
      601 atctcctctt agttttttta gagttaaaaa tacattttaa aagattaaat tttaagttat
      661 atatttaatt ttaactctaa ggggttaaaa tttcaattaa aacaaataaa attaaataat
      721 aaaacaatat attttatttt taaggttaat attaaatgtg aatatataaa aaataacctt
      781 aatattattt aatttatgtt ttatttgtat aaaagtatcc ttgtaagtaa aatggtatgt
      841 acattgaatg aaaaggagta tgaaaaatga aggtagtaag taatattcgt atgattatgg
      901 cgaaaaagaa tatagataat atttcggatt tggtgagaat aacgggagta agtcgtaatt
      961 ctattaataa gttgtggcac aatgagaatg tgtcttcttt aaaattagat acattgataa
     1021 cgatctgtga gaagttggat gttgagttat tggatttaat tgaatatatt cgagatgata
     1081 gtgagacaaa ataatttagg ggctatttta gctttagata gcttgtgtat gatgaaagca
     1141 tatttttgct aatcgacaat gagttatatt cacatatgtt ttttttgtag ggctatatgt
     1201 gtatttaaag ttttgatttc tatttgttat tgtatgaata tggtttgaat gtggtaaatg
     1261 aaattatagt cttgctaggt gcgtcaacac cttggaagat gagcggtact ggcatacttc
     1321 gtgatttcat tgatagtagt gcagtaaagt gatggaattg atgagtattg aaaaagagtt
     1381 cctgtttggg ggctcttttt ttatttgtta ctaagtattt ttaaatagtg aaggtgctac
     1441 actatttaaa aatacagaat attaaaaact ttatatatca actgttgaaa agttagtctt
     1501 atacaatagc atgataacat gatagaaaat aaaatataga taattttgga aaaggagaag
     1561 attctatgaa tgttcaacta ggaaacgaac aaattacaaa attattaaat gattggtatc
     1621 aagaaattcg agcgcaacac attattaaag caaaacaatt aaaacaaaag attgaaaaag
     1681 aaattaacaa catagaagaa gaccaaaatt tacttttata ttattcactt attgatttga
     1741 gatacaagat tcttacaaac gactatgcag atagagaaca atctttagaa aaaatcgaac
     1801 aattaaaaga acacacaaat aattttttag attactatta tcatttcttt aaaggcatgc
     1861 atgcaatgaa aacaggaaat tatagtgaaa ctcaaaaata ttatgatata gcagaaaaat
     1921 tactagaaga aattcctgat gaagtagaaa aagctgaatt taattatgct gttgcaactt
     1981 tctattatca tactcatcaa gccttattag caactcaata tgctaacaaa gccaaaagtt
     2041 tttttagtgg gaaactagga tacgagatta aaaccggagc atgtgaaaac acacttggta
     2101 tggcttgtat tacattaagg gaattttctg ttgcagaaga atacttgctt tccgcaatta
     2161 ataaatttga aaaacacagt gaaaacaaat tagctttaat cgtacgttat aacttaggtc
     2221 ttttatatgc agatcaaaat ctttctgaac ttgcaatccg ttatcttctt gaatcctttg
     2281 aaaatcaaga agacgattac aaaacaatgt ttttattagc acaagaatat tacaagttaa
     2341 aacaaacaaa tacagtaaac acatatattg aaaaagggct aaatgtttgt aaccaagaat
     2401 ataaaattca tttcttattt ttacaagcat taaacaataa attatctact gaacaattag
     2461 aaaaagtcat gttagacgcg attccatatt ttgaaaaaca aaatctatgg aaatacatac
     2521 aagattatac tgaagaattg gctataagat tttatgaaga aaagaataag gataaatcta
     2581 atgaatattt ttataaaagt tacaaagcta aaagaaactt attagaaagg gagtcattga
     2641 aatgatgaaa aaatttagtc tgatactaat aggggtagca tgtacaacag gtattttctt
     2701 ttcccaattc aataattcca ttcaaacaca tgatgctaaa gaaaaaaatg atattattca
     2761 acaatatgca catggaaaag atatttaaaa gcaaattcca acaattagca atcgacctaa
     2821 atattaggat tattaacaaa atattgaatt atatgtaaat taagtttatt atctattttg
     2881 aataataaac ttaaggggat gaaatttatg aaaaaaatta gtattgagag aagtaattgg
     2941 agaaaaacat ataaaaaaat gattccagta ttgttagtcg gaggaatatt ggcaggaaat
     3001 gtagcatttg tgacaaataa tgtttttgcg gcaactacaa ataacaacaa tataatacaa
     3061 acgaaacagt ctctcgtttc tggtcaagat gaaaagagca ttaatgaatt agcagaagcg
     3121 tttcaattta tgcatgaaga agccgctata tataataatc aaggtgaaat tgttgatttt
     3181 gattttaata aaatagaagc gaaatatggg aatgatgttg gtttagatgc acttaaacaa
     3241 caaattaaaa aagataaaag tgatattgct ggtttatctt ttacaagttg catgaaagat
     3301 gctttatatg acttttttgg agtaaatgct gtacaagccg catttaatgg tggattggca
     3361 tattatcttg agaaaaaatt atggaaagaa gcggctaaaa taggcgttaa attctttata
     3421 ggaagtagcg tagcaggttt agcggctact ttagcttatt atggaggtaa gtgtgcaatt
     3481 tatggataaa agttcaattt taaaacaaat gtattttatt ttatttccgg taacactttt
     3541 tctatctcta aatatttatc aatggattgt taaaagtgat tactcattta caggatatat
     3601 aatccaaaat ttaattattg tagcattagc gttagtagca gctatcatag cttatttaat
     3661 tactaaaaaa aatatttaac acataatgtt agtctaaata acagaaaatt ctaaccctta
     3721 ttcttatttt gtattatact ggttatgtac aggtaaaatg gtttacaagt taatttttcc
     3781 tcgtttcaaa aaaagtccat tcttttcaaa agaatggact tttttcttgt tatatttgct
     3841 gtaactatta aaagggcatc aaaacgatct acaaaagcct caagacgacc cacaaaccat
     3901 ctttaaacgg tctcaaaacg aactacaacc cgaaaaaacg catcccagtt tttttaggtt
     3961 taattttaag cgcataccaa gcgcatatag gcgcatacgg gacacataca aagtgccgaa
     4021 aaacgcatat aggtcgcata caaacggtag aaatacatcc ctaaaaatag agtgatagaa
     4081 acccaatagg cacagcctat ttcaccactt tttttgcaaa gtgggattcg gtttccttat
     4141 gctctttttt tatttttgct ttttaagggt cttataatcg tttttaagta ttttaggatg
     4201 ttgggtttct taagggaact agaagttcct agaatggatt ctgagcgttc tgaggtgtat
     4261 ttttctatgg attcgatatt ggaattgctc taggaatagt tgtcgtttta ttctgtggct
     4321 gttccgacag tggttctcat aaagaatatg attgtatcaa tagatttgat gatgtttgaa
     4381 aaatagagaa tagttgaagc tcttcctgta aaacaaaaaa aattatgttt gtagaggaag
     4441 tagtcatttc actacaagaa aattcccata gatcggtggc gagttgaaaa atgttgaagg
     4501 ttggcgagga cattttacga ggacgcggga atggctttgt cattgttggg gttaatagtt
     4561 tttaagaaaa gttggttagt agctgaaaaa aatagggaag attggcgagg atgtattgca
     4621 tggcagcggg acatgttatg tagtcgtggt gaatataaaa agagactttt taatacatgg
     4681 atgtcaactg taaaaagtta gaggtagttg tatgatgttt gtccaacggt aagcggaacg
     4741 aagtgagcat taaggagtgg acgaacccac tgtgcccaga accctcttgg aactccaaaa
     4801 cgttagccgt tttggggatg ccaaccggcg agggagtccc tcaaggattg aggggttggg
     4861 gagttcgatt gaaaggggtc aaggggaaga gtttcccttg tgggtttggg caaagcccat
     4921 gatttttttt gccatgcttg ttatggctcg gcggtagccg aaatgtcccg aagggcacac
     4981 gtgtttacgc agtaaactgt agtgtgctac tgtttacatg gaactacccc cgaaaaaaag
     5041 gagcgaggcc catgtcgaag ttcgtgattt gccatatgca aaaatttaag atgagagatg
     5101 tgaaaggatt acaaatacat aatcaacgtg aaaaagaaag tcattccaat tcagatatta
     5161 ttcaagcacg aacggaacag aattatgatc tgattcatga caaagaaaaa gtggattata
     5221 agaaattaat tcaaaatcga attgataatg gtgttgtatc taatcgtgca attcggaaag
     5281 acgctgttgt ttgttgttcg ttcatgattt cggctagtcc ggattatatg aattctttat
     5341 ctcaagtgga acagagacga ttctttgaag agtctcttcg ttttttgaag gggagatatg
     5401 gagaagagaa ctttgtgtat gcttccgtac atgttgatga aaaaacacct catatgcatg
     5461 tgggtatggt tccagttaat gaaaaacaaa aactatcagc gtattcattt tttaaaaaca
     5521 aatcagagtt tcatgattta caagataaaa tctatgaaca tgtgaaagaa aagggatttg
     5581 atatagagcg tggtgtttct agtgatagaa aacatttatc gacgcagcgg tttaaagcgg
     5641 tgactttaca acaagaaata gaagaactag agcaagaaaa gaagggaata gatagtcgtc
     5701 ttcatgattt aaaaatgtct ttagatcaag caaaaagtgt ggatgagatc ccagttaaag
     5761 aaaaaggtgg gtttatacgc tctaaaacgg tcgagattac ctcggaagac ttcgaaagta
     5821 taaaagtatt agctaggtcg tcggaagccc ttagagaaga tgttagacgc cttaaaaacg
     5881 aatcaggtgt aattacacgt gaaaaagatg ggctgtatag agagaaatgt attttagagg
     5941 ggcaaataac agaattgaaa agagagaatc gaggattgaa ggaagagaat gatttcttta
     6001 agaaaacgtt ggaacgagtg aaagagttgt ataaagaaaa gcttcctgaa ttggctgggg
     6061 tgattggtta cgttaagggg agtatattgg acaggatgaa ttgcaaattt ttaaaacgat
     6121 attttactag tgatgatgaa gtgagaggcg ctcaaaagtt tttgaatcat aaacaagagc
     6181 atgaagaaca acaaaagaga ttaaaacaag tgcgaagtag ccaacagaaa aatcgggacc
     6241 agggattgga gagatagtgt ggttctgttt gagttttttg ttttttagat ttatagcgtt
     6301 agcggaattt gttttttata acttgcttaa gatgagtatg tttctcagat gttttatccg
     6361 ttaaaaaaaa tggttttgaa cgaaaaaaaa tgcccctgtc cacggagtgg atttaggggc
     6421 atttttttat gatttttcgt gttcgaaaaa tcgagttttt tctttcttta atacataggt
     6481 aactatttcg gcaacgaaat tgatgcctat gtattctttt agcccttatg tatcaagggt
     6541 ttgtaaaaaa aaagacttgc tttttcaggc ggatagattt atcattttta gtgtcgaatt
     6601 aaaaatcata aataccacga gaggagcaag tccaaaaaaa ggattcgtac accctttttt
     6661 atatcgaata tgaaattcag aaattagaaa ttctgatgtt tttcagatag gactaatata
     6721 tcaaatatgg caaagaaaaa tcaagatgaa atcactggta aattacaacc taaaaagaaa
     6781 cagaatatca agatttacga atttataaag aagaaaatga gtgagagcgg gaaagaattg
     6841 tttaaatcgt gcagtatctt taatgaattt ttggctacaa aggataaaaa gaagaagaaa
     6901 cgagtaaagg ggaatgattg taaaaatcga ttttgtccga tttgtgcttg gaggaaagca
     6961 gggaaggacg cggtgaaaat cgcaacgatg atggaagcaa ttaagattga ggaaaagaaa
     7021 gagtttcttt ttttaacgtt gacgactcca aatattaaag ctgatatggt gaaaagtgag
     7081 attgatagat ttaataaagc ttttaaaaag ttatttgata gacgtaatat tcaacgttct
     7141 attaagggtt atattagaaa gctagaaatg acgtatgata aagaacgatt catcactgaa
     7201 gaaatgtata acaacaaaaa gagaaaagca tattatgatt ctcgtggatt gaaagtagac
     7261 gatcataacc cgaattatga tacatataat cctcattttc atgtattgct ttgtgttgat
     7321 aaaaattatt ttaaacgtaa agagctttat attaagcaag aagaatggtt agaaatgtgg
     7381 cgagaagtaa cagatatgcc agagattacg caagtacata ttcaaaaagt agagttaata
     7441 cgagaaggaa atgcagttgc ggaggttgcg aagtattcag cgaaagacta tgaaatgtcg
     7501 gttagtcaag aagtgtttga cgtgttctat ttagcattaa aaggtcgtca attaattgtt
     7561 tacggtggat tattaaaaga ttatgcgaaa aaatatgaag atggcgaatt ggacaagtat
     7621 aagagtacag acaagaatga atattattat agattaatcg caatgtggaa taaggattta
     7681 atgaaatttg aacaagagta tcaagagtta tctgagagtg agaagcaaga gtataacggt
     7741 catttaatta atgaagtaga tgtggaggcg tagtgtatga aagtagcggg agatttatat
     7801 tattattgtc ttggttgtaa aaagtttcat gaatatgaaa aaattgatca taaaggtgta
     7861 aatagaaaat tatgttttta ttgtttcaag attcaatcta aaaaaacgaa aattattggt
     7921 gatgctgaag gtagaatgca aatttgtgag acttgtcata aagaattgtt ttagttggtt
     7981 gatttgtgaa aaaagcaaag tcaaatgtta gaaattttta ataagggaac tgactgttat
     8041 atcaagtcag ttcccttatt ttattgagtc tatttttgag tgcagcaagt agcattcaaa
     8101 agttttttta tgatttaaca aaaaggagtt tttcttaaat ttaaatttcg tgaatttgta
     8161 aatttatttt taaagtttat ttcgagatat attttgttct actaattttg ttttactaac
     8221 gtacgaggtg aaaacttaaa gtgtgcgcat gaattttttt agtaatatat attacgataa
     8281 aataccgcac gttttttgat gatttttttg aaaatatagt tttgagaaat tctaaatttt
     8341 cggggctgat tttggatttt ttatgtggga tttgattttg gaatttgtta aattggaaat
     8401 ctcgattttt cgattttcgg aatttttgtt ttctcttttc tttgttattc tgtatttcga
     8461 aattaaggag tttatataat accttcatga cgaactgaaa acgaaaaaac ttt
//
