LOCUS       NC_020182               9294 bp    DNA     circular CON 23-DEC-2023
DEFINITION  Klebsiella aerogenes EA1509E plasmid pEA1509_A, complete sequence.
ACCESSION   NC_020182
VERSION     NC_020182.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3138432
            Assembly: GCF_000334515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes EA1509E
  ORGANISM  Klebsiella aerogenes EA1509E
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Diene,S.M., Merhej,V., Henry,M., El Filali,A., Roux,V., Robert,C.,
            Azza,S., Gavory,F., Barbe,V., La Scola,B., Raoult,D. and Rolain,J.M.
  TITLE     The rhizome of the multidrug-resistant Enterobacter aerogenes genome
            reveals how new 'killer bugs' are created because of a sympatric
            lifestyle
  JOURNAL   Mol Biol Evol 30 (2), 369-383 (2013)
   PUBMED   23071100
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Genoscope -,C.E.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2012) Genoscope - Centre National de Sequencage :
            BP 191 91006 EVRY cedex - FRANCE (E-mail : seqref@genoscope.cns.fr -
            Web : www.genoscope.cns.fr)
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/23/2023 16:33:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,426
            CDSs (total)                      :: 5,302
            Genes (coding)                    :: 5,149
            CDSs (with protein)               :: 5,149
            Genes (RNA)                       :: 124
            rRNAs                             :: 10, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 84 of 153
            Pseudo Genes (incomplete)         :: 66 of 153
            Pseudo Genes (internal stop)      :: 14 of 153
            Pseudo Genes (multiple problems)  :: 10 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FO203353.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella aerogenes EA1509E"
                     /mol_type="genomic DNA"
                     /strain="EA1509E"
                     /db_xref="taxon:935296"
                     /plasmid="pEA1509_A"
     gene            151..414
                     /locus_tag="EAEROS_RS00005"
                     /old_locus_tag="ST548_p2001"
     CDS             151..414
                     /locus_tag="EAEROS_RS00005"
                     /old_locus_tag="ST548_p2001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            404..703
                     /locus_tag="EAEROS_RS00010"
                     /old_locus_tag="ST548_p2002"
     CDS             404..703
                     /locus_tag="EAEROS_RS00010"
                     /old_locus_tag="ST548_p2002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(765..1142)
                     /locus_tag="EAEROS_RS00015"
                     /old_locus_tag="ST548_p2003"
     CDS             complement(765..1142)
                     /locus_tag="EAEROS_RS00015"
                     /old_locus_tag="ST548_p2003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            1211..1390
                     /locus_tag="EAEROS_RS00020"
                     /old_locus_tag="ST548_p2004"
     CDS             1211..1390
                     /locus_tag="EAEROS_RS00020"
                     /old_locus_tag="ST548_p2004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1418..1948)
                     /locus_tag="EAEROS_RS00025"
                     /old_locus_tag="ST548_p2005"
     CDS             complement(1418..1948)
                     /locus_tag="EAEROS_RS00025"
                     /old_locus_tag="ST548_p2005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1955..2686)
                     /locus_tag="EAEROS_RS00030"
                     /old_locus_tag="ST548_p2006"
     CDS             complement(1955..2686)
                     /locus_tag="EAEROS_RS00030"
                     /old_locus_tag="ST548_p2006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(2686..4650)
                     /locus_tag="EAEROS_RS00035"
                     /old_locus_tag="ST548_p2007"
     CDS             complement(2686..4650)
                     /locus_tag="EAEROS_RS00035"
                     /old_locus_tag="ST548_p2007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6131..6280)
                     /locus_tag="EAEROS_RS00040"
                     /old_locus_tag="ST548_p2013"
     CDS             complement(6131..6280)
                     /locus_tag="EAEROS_RS00040"
                     /old_locus_tag="ST548_p2013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6365..6622)
                     /locus_tag="EAEROS_RS00045"
                     /old_locus_tag="ST548_p2014"
     CDS             complement(6365..6622)
                     /locus_tag="EAEROS_RS00045"
                     /old_locus_tag="ST548_p2014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(6632..8317)
                     /locus_tag="EAEROS_RS00050"
                     /old_locus_tag="ST548_p2015"
     CDS             complement(6632..8317)
                     /locus_tag="EAEROS_RS00050"
                     /old_locus_tag="ST548_p2015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8720..9139
                     /locus_tag="EAEROS_RS00055"
                     /old_locus_tag="ST548_p2017"
     CDS             8720..9139
                     /locus_tag="EAEROS_RS00055"
                     /old_locus_tag="ST548_p2017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     BGC             1632..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5450..6028
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1959..2686)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 catggcatat gttttgacgt aaaatggatg ccgtaagccc ttttttaccg actttctgct
       61 tcatcagcct gactgacacg accatattct ttcagaccgc cccctgctaa acttccttca
      121 tattgccgca ttaacccggg aggctccgca atgagcaaca ctacacacta cgaaaacgcc
      181 aactttcttc gggaactggc tgaaagcctg ccccgcatac tgccggaagg tggcccggac
      241 aaagctgcgc tgttgcagcg actggctaac gaagaactgg cccaggcaga gtatgaagat
      301 caggttcgcg ccaaagtgac cgccgcccgt gctgacacgc gtccgggtat gaccacggaa
      361 caactccgcc agcgcctgca tggccgttat caggaactgc gcgatgccgt atgacgtgga
      421 atggaagcag ggagccatcg aggatgtaac gacgctgttt gactatatcg cggaaaattc
      481 atccctgtgg gatgcgcaga acgttaccga ccgggttctc gctgcggcgg acaaactggc
      541 cgacttcccg cgactctatg aagtggactc acgctatggc gaggacgttc gccggatcag
      601 tctgatggga cagcatgtcc tctatgatgt tgatgaccag accagaaaag tccgggttct
      661 ggccgtggtc ggccagcgcc agaatcccca tattgttctc taataaccgg tgacggggac
      721 gcaattcatt gcgtggccgt cgccggttgg acaagcacag cgcgtcagcg ttttccccac
      781 ggccaccacc ggcgcggtgt tccggccggg gtctctgctg tctccggttt gtgttccagc
      841 agtctcatgg actgcatttc ctgccgtagc ccggccacct cctccctgag tgcttttatc
      901 tccgcgatca acgcgtcgga ggccattttt tcagtctcac ccgacagtct caactcgtcc
      961 cgctcgtgag tctcatcctg tcttaactca ccgtacaccc ggataagctc gctggtatcc
     1021 accacccggc cgccatcagc ttccgtacgg tatgatacgc ggcctttcgc catatcccga
     1081 tacagggaac ttcgggcttt acccgtcagt tccgtcgcct gtctcaccgt cacccacgcc
     1141 attgtctcac cccgctattt ttgagacagt atacctgaca ggcgcacacc atgcccggaa
     1201 ggaaagaatc atgacaaaac aggaaaaaac agccctcaat atggctaaat ttattaagga
     1261 gcagtcgctg ctattgctcg ataaactgaa tgagctggat atggataccc cggccgacat
     1321 gtgcgagcgg ctacacgagg atgcggaaac actctggcag gtgatgcggg ataagctcgg
     1381 ggaggaataa cagcaccggc atggccggtg ctggcattta ttttttgccg aacggccacc
     1441 accggcgggc aggtttgtcc tccggcagtt caggtaccgg cacgttaaaa tagcggctgt
     1501 tatattcccg catcagctcc gctttcagct tctcgttcac cgcgctttcc ccgtgccgct
     1561 cactcaccac gtcaatcatc gcgttaatct gctccggtga gcacttcccg gcatcaacca
     1621 cctgcacacc gttctcgatg gcgaaccgct ccaccttgcg atagaggtaa tcacacagct
     1681 tcattcctgt gggtttgttc cgttcccatt gcggattttt accagtgaaa aagtacgtgg
     1741 ctttttcgtc gctgttgagc cgctcgtcat acatcccgat ggatttcatc atcgccagat
     1801 gctgggcccg gtcgacaaaa cagaaaacat acgccccggt ctgccaggcg agcaccacca
     1861 tatcttccag atgctcgctg gtcggcatga tgcagcgctc gccgttccgg tcctccgcgt
     1921 cttcaataaa taccatgtac agcgccataa tccctcactc cgttgtcata aatttaaagc
     1981 acgcaaacaa ccgttcacga tccagctctg ccgttttgcg ggcctcgccg gtctgtgggt
     2041 tcacgatttc atccaccgac aggattgccc gctgcaccct gacctgcgtc gcccggtccg
     2101 ggcagatgta ccagatttca tcgatgccgt tagctttgcg gttgaacaga tacccggaca
     2161 ccacttcctg ataccggcga cggctcttga cggtgcgctc cacctcaaac gccaccgttt
     2221 taccgtccag ctccgccagg gcgtccggca ctttcatgcc tttaagactc agacggtgca
     2281 gaatgcgcca gccggtggcc cctctggctt ccatatcaag gcgcagggat tgcacggcca
     2341 gccggtgcgg cagatgtgct gccgacaccc ttgacggctg aaacggaata acatccgcca
     2401 gcggttcgtc ctcgtcgaac gagaacgcca cgccatgcgg cgtcagcccc cacacgctga
     2461 cctgacggcc gtccaccgga tatttggtcc ggaccagcag ccctttcgcc accaggctgt
     2521 cgaggaagcg gtagaccggc tgcaccgccg caatccccag ccgccgcgca accagctccg
     2581 ccgtggaaca cagctcttcc cgcaaccagc gcagggtcag ccgggttttc tccgcgttcc
     2641 gcgcctgtcg tttcgcgtgg ctgacgttgt acagctccag cgccatcagt aaatccccgc
     2701 aaaatcatca tccgctttcg gcccgccgcc gtcggccggg ggccggaact ccgccagggg
     2761 atcggttttc gccagcaccg ggaaacgctg caacgggatt tcccggcgct caacccgcag
     2821 cggactgctg aacgccatcc gggccacacc cagcccggtc atgaccgcga acccgtccgg
     2881 caggtgttgc agggtgttca cgtcaaacag cggccgcgcc tccttctgga tgaagctgtc
     2941 cccgctgata tgctccacgt tccccgcctc ccggctggca cggcggcgct ccacgtccac
     3001 cctgatttcc ccggtctgcc ccgccgccca ctgcgcggac tccaggctgg ccgcccggta
     3061 aaaccagcgg atcggcgtgt tatccagtac cgtggttttc acaaattcag cgggcagatc
     3121 ctgcccgcac tgcccgaaat cgcccagcga ctggtgcgcg agcaacagat tgcagtcccg
     3181 gctgcgcagc gtccccagtg cgttcagtac atacttcgac agcagatatt taatttcatc
     3241 gagcatcacg ctgacgtgtg gccactcccg gaactcgtcc cgcgcaatga tgatttgcgc
     3301 gcagcgggca aacagcattt tttgcacacg gatcaccgcc tcgtcgtcca tcgagccgat
     3361 aacgtacagg cagccgccgc cgttgataat ccccgccaca tcgatgccac tgtccgtctg
     3421 caggacgctc aggtcagcga cgttttccag cctagtgatc aaccccttca cgtccttttt
     3481 cagggcctcc ggcagcgcgt acgccgccgc cagcacctgc tgaatgtttg ccccctgcgg
     3541 aaactgctcc gcaatcagct tcgccgcttt ctgctcgctg atgcggtagt ggtccgccac
     3601 gtcgccggtc tcgcccaggt taagcccggc aatcagcagg tttttcagcg cgtactggtc
     3661 gagatcgcgg aacagattca gctgcgccgg tttccccggc cgcagatcca gcagggtgaa
     3721 gtccggacag tgcgcccgga agacgtgcgg cgcccatgcg tcccctttgg ggtcgaagta
     3781 caccaccgcg tccccgaaat cgcgtacgca gcgaatgagc gcgttggtgg ccatcacgct
     3841 cttgccgctc ccgggcaggc cgaggatctg aatgttggtc ttgcgccagg tatcacgcgg
     3901 aaccagcacc ggcttgccct gctcatcctt acccagaaac accgcctgct cctgccgggc
     3961 ctgctccgag gaaaaatcgg ccagcgcatt aaccggtacc ggctccccga aatgcaggtg
     4021 gcgcacgtcg gtcatctctc ctgtccgctg gctgctgcgc cggctctcct gattcagttt
     4081 cgcgttcacc gcctcgtcag cagccgcaaa atgaccgtca agcagcagga acgccagcag
     4141 ccgcgccaac acaatcccga cagccagccc gcccgcgcac caggcaatcc cgaacaggtt
     4201 agccggctgc agcaggtaat aatctgcgtt tgcgtagagg tccggacgca ggacggtttc
     4261 tccccagctc tcgataaaca cggcgacaac cagccccccc agaagggcca gaccggccgc
     4321 gccggtcagc cagtacgcgt agttccagac atgtcgacgt gccctgacca tccgcccggg
     4381 cgaatccagc gaggccagca ccgaaaatgg cgctggccgt ttattatcgc gcttatagcg
     4441 atcggcatac tcccgtcgct ccttatgact cagccccaca aaacgcatcc gtccggccca
     4501 aagttcagcc agaccgaaaa acaggccgat aactaggaaa gccccggcca gaccgtaata
     4561 ctcctggtac agccagctga cccacggata aatctgccgg gccagctccg gaatgtgcca
     4621 cttccaggca agcgaatccg tattaaacat gttctctctc cttcagacga tggtttgccg
     4681 gcgtgatgtg cagcccccgc tgcacaagcc gcggacatgc cccaaggcat ggcggcggcg
     4741 gtcacgtccg tggtgattgc gttcagcttt caccacggag gccagcctct ttccgcccca
     4801 tgcggaaaag gcgcagcccc ggaggaacaa cgagcacgcc ccagcgtgtg agaggaaaag
     4861 gcggcacccc agtgcggtct tttccggtgt tccggccccc gtcagagttc gccatagcga
     4921 gcggctgacc acccgcaaaa tcaggagcag ctttgctgct tcgccaggtg cgtccagcac
     4981 cgtggatttt tgtgggtggt caggcgtgag cctgacgggg gccgggtgaa tttctggcgt
     5041 ggccggcgtc cagccggacg gagccagaaa gagggggaga gaccgtgcgt cggtggcgtc
     5101 cagccagcgt aagcagggga tatccccctg aggtcagcca cgccctcgcg gggatgacct
     5161 gtatttctcc gtaaggagaa aaaagcaaca gagataggtt aacgtaaaat gttcagcgaa
     5221 aaaccgatat gattcaccta tcctggtaat ttattatcag ggaatataaa gacttaatca
     5281 ggggacaata tgccacatca gcgcgtccgg tgtccgtctt gtaggtgccg gggaaattgc
     5341 gcctcctcgc tcactcggtc gctacgctcc ggccgtgaga ctgcggcggg cgctgcggac
     5401 acatgcaaag ttacccacag attccgtgaa taagcagggg actaacatgt gaggcaaaac
     5461 ggcaaggcca cgcaggtggc gtttttccat aggctccgcc ctcctgactg agttcacata
     5521 aacagacgct ttttcggtgc atctgtggga gccggtggct caaccatgaa tcagtcagga
     5581 gtggcgaaac ccgacaggac ttaaagatca tcaccgtttc cgatgaatcg ctccctcttg
     5641 cgctctcctg ttccaaccct gccgtttccc ggatacctgt tccgcctttc tcccttgcgg
     5701 gaagtgtggc gctttctcat agctcacact ctggtatctc ggctcggtgt aggtcgttcg
     5761 ctccaagctg ggctgtaagc aagaaccccc cgttcagccc gactgctgcg ccttatccgg
     5821 taactgttca cttgagtcca acccggaaaa gcacgcaaaa cgccactggc agcagccatt
     5881 ggtaactggg agttcgcaga ggatttgttt agctaaacac gcggttgctc ttgaagtgtg
     5941 cgccaaagtc cggctacact ggaaggacag atttggttgc tgtgctctgc gaaagccagt
     6001 taccacggtt aagcagttcc ccaactgact taaccttcga tcaaaccacc tccccaggtg
     6061 gttttttcgt ttacagggca aaagattacg cgcagaaaaa aaggatctca agaagatcct
     6121 ttgatctttt ctactgaacc gcgatccccg tcagttcaga ggaggacgat ggtgccaccg
     6181 ttccaccctg aacatcccgg atatagtttg cctgacatgc gaccagaaga aagcctgaaa
     6241 aaatcaatat aaaaagaaaa acaaccttta cctttttcat ataccttccc tgtatttaaa
     6301 aaccccggta ttcatccctg aacaccggaa gtctgtattt caaatcatcc ttgataactt
     6361 ttgcttacca gttaccatcc cggtaaacaa aggatacaaa gtaatcaaat ttactaatat
     6421 cgattacatt tttaaagtgt ggctgtaata tcgaaaccca tgatttttca acatcaaacc
     6481 aaccattatt aatattatcc tttaaaggca tccccagact ttcaatgaca gaaccatcat
     6541 caccgaagtc ttttgagtat tcaccacctt taaactcttc ggttttctta tcaaaccaat
     6601 gaatatgtaa tttaagcccc atatttacct cttaaagata ttttttaatg tttcgttttg
     6661 gatccggccc tttaacctgc ttacccgttt ttggatcgaa tgccccgagg tgttcgccat
     6721 cactggcgcg gtacccttca agctcaccgt gctgggaatc ccattcataa attttacgct
     6781 ttttatcacc ataccatcgg gcccgcttac caccaccacc ttgtttaggg gttttaggtt
     6841 taccctcttt caactctccc aaccccttaa tatcttcagt cttgggatca ggaaaataat
     6901 catggccgta atctttagtt cccttacgag gttttttctt ttcctcattt aatttatttt
     6961 ctgcatcctt cgcttttttc tccttttgtt tccggctctc catcgccccg gaaagcgcag
     7021 ccttatccct ggataaggat tcattcattt cgtttaaaga tgtctgggca gcttttaatt
     7081 tctcattctg attctgaata tccttatcaa gatagcgaac ctgatcctgc cacccggagc
     7141 catattcatg aggcgggtta taagtcacaa aatcatcttt atttgcctct gcctctttta
     7201 cctttttatc ggcttcctga acagcagccc ttgcaccggg gatcgccttc tctgtcgatg
     7261 caatgcgact gtttaaggtc gcaatatttt tatcttcagc atccagctca gctttcgctt
     7321 tatcatactc tcttttcagc ccctcttccg ggtgagcagc gtcccatgcc tgctggcgac
     7381 gcttttcttc ctccagacgc tgttttacct gtgccggagt aagaacatcc gtcaccgaca
     7441 catagaccgg tttctgtccg ctctctttcg ggaaacgaat aacggcttca cgggagttac
     7501 caccggccgt aaaaccagcc ggacgtgaat cacctttttc agcaataatg ccttttggcg
     7561 gggctttcgc ggccggaaca cctttaggta cgctcacctg cagagacggg agacccggaa
     7621 tcgacacact gaataccccc ggacgttttg tcggtttcgc atccaccaca ggaacactca
     7681 ttggccggcc ggtgacaacc gcaatatgct gccgctcgtc cttcacaata tccgccacac
     7741 gtttagtgac gctgaccgtc gcctggtcca gcggcaggga gctgaccggc gtctccgtta
     7801 ccgtgtctgc cggcaatgac gtcacgattt ttgacatcat tttcggatca tcttttgcta
     7861 tctcagaagg cagcactccg taaatcgcaa tcccccacag accaaactta aacggacctt
     7921 tcagggcagc cagcacatca gcaacagcgg ccgacaacgc atcgccggaa acggataaag
     7981 ccagtccttc agcccccgga gtggccaagg caggcagacc gaaggccatc gcggtggccg
     8041 aagactgtcc gccaccagag ctcccgctat gattgccgct gttaccgcca ttaccaccgg
     8101 gtttactccc gccgttacca aactcccccg ggttatagtt atgaatatcc ccgttaggtg
     8161 tatgcgtcgt accccagccc ggcccgctgt ttggatccgt accaccacct gatgaagggc
     8221 cacctttacc tgatgtaccg ttcacattcc cactggcctg accaccattg tgtcccagac
     8281 ctgaattacc cggacctcgt ccgtctccgc cactcatata cttttcctct ttgactttta
     8341 aaacaataaa ctaaaataaa atactgtaca tataaccact ggttttatat acagtataaa
     8401 aattacgctg ccttgttttt catgtcaagt ctgtaaattt gcgtttttgt gaaaatgatc
     8461 aaaaaaacag ccactcacac catgcgcaat cccctccaga tgcaacaaaa accagcacaa
     8521 acggcgccgt tctccggcct cagtatgttt tactgaaatc attatatgag ataccagaaa
     8581 gccccctccg ggacaggaaa agaaacgggg ggtctggttc gggttgttcg gtcactgaac
     8641 acggtcagaa actgatgata agatagcctg cggatatata actcaatgag aaaaaagaat
     8701 taaaggtaag gagacgctca tgagacgttc cggcagcaaa ctgtgtctta tcgccatgat
     8761 aacagcgaca gcaactctca ctgcctgtgc tccaaagggc agcgtggaac agcatacccg
     8821 gcattacgta tatgcttcag atgacggttt tgatcccaat ttttccaccc agaaagctga
     8881 cacaacacga atgatggtgc cttttttccg gcagttctgg gatatgggcg caaaggataa
     8941 agcgaccgga aaatcacgga gtgatgtgca gcaacgcatt cagcagtttc acagtcagga
     9001 atttttaaac tcactccaag gcacaacaca atttgcgggt accgattacc gcagcaaaga
     9061 cctcacaccg aaaaaatcaa ggctactggc tgacaccatt tcagctgttt atctcgatgg
     9121 ctatgaaggc agacagtaag tggatttacc ataacgcgtc attacgcgca ccgctcagac
     9181 gggctcaggg cgtttccggc ggctacggga taaaacaggc aacaaccccc ccggaaaact
     9241 gccggaaacg cgcccgataa attttatcct aatgcatgcc tatgcacgca actg
//
