LOCUS       NC_019988               5225 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae plasmid pB1019, complete sequence.
ACCESSION   NC_019988
VERSION     NC_019988.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226175
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   San Millan,A., Santos-Lopez,A. and Gonzalez-Zorn,B.
  TITLE     ColE1 plasmids and antibiotic resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   San Millan,A., Santos-Lopez,A. and Gonzalez-Zorn,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2011) Sanidad Animal, Universidad Complutense de
            Madrid, Avda. Puerta de Hierro s/n, Madrid, Madrid 28040, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 01:44:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 6
            CDSs (with protein)               :: 6
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JQ319775.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BB1088"
                     /db_xref="taxon:573"
                     /plasmid="pB1019"
     gene            271..1521
                     /locus_tag="HS870_RS00005"
                     /old_locus_tag="D647_p17001"
     CDS             271..1521
                     /locus_tag="HS870_RS00005"
                     /old_locus_tag="D647_p17001"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160339.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001584442.1"
                     /translation="MTRPIAVDLFAGAGGLSLGFEQAGFDIMASVEIDPIHCSAHKYNF
                     PMCKTICRSVVDVTGEYVREVSGIGEKEIDVVIGGAPCQGFSLIGKRALDDDRNKLVYH
                     YVRLVLELKPKYFVFENVKGLTVGKHKQFLVEIMDAFKDGGYDVVSDYKVLNAADYGVP
                     QDRRRLFLMGGRKGIALPSYPEPLSIRTTVGDAIGDIPDAEKYPELWGRDWVYAKFGEQ
                     SLYSSYLHGVGKDPSDFSYPRIFNKDKLTCSLLTDHSDISRGRFVETLPDTVEPISRFK
                     KLSMNGLCNTLRAGTASDRGAFTSPRPIHPIYPRVITVREAARLHSYPDWFRFHVTKWH
                     GFRQVGNSVPPMLARSVGLQIMKVLGVTPKKPTKKIKLGDESLVAISMSDAASMFGVSS
                     NVIAKRIKNSNKEVVCE"
                     /name="DNA cytosine methyltransferase"
     gene            1514..2323
                     /locus_tag="HS870_RS00010"
                     /old_locus_tag="D647_p17002"
     CDS             1514..2323
                     /locus_tag="HS870_RS00010"
                     /old_locus_tag="D647_p17002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032446875.1"
                     /translation="MSKDKKERSKNRYHQLVEKIFFDKYVDGDLEVLFERLDINSAAKD
                     LGIELPSNVGDVIYSIRFRTPMPESILLTQPEGKEWIIELAGRSKYRFRLVTISRILPN
                     PNMVAIKIPDNTPEIITGYSLDDEQALLAKVRYNRLIDIFLGITTYSLQNHLRTTVKGI
                     GQIEIDELYVGIDKYGCHYIIPVQAKGGTDQISMVQTSQDTAWCEQKYPNVRCRAISAQ
                     FVSSDLIALFELKIDEGQVKLIEERHYKLVPVKELDSSDISNYRMSV"
                     /name="hypothetical protein"
     gene            complement(2543..2674)
                     /locus_tag="HS870_RS00035"
     CDS             complement(2543..2674)
                     /locus_tag="HS870_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072007956.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPKRHAKAPLAAATGNRIHGAE"
                     /name="replication initiation protein"
     gene            3306..3647
                     /locus_tag="HS870_RS00015"
                     /old_locus_tag="D647_p17003"
     CDS             3306..3647
                     /locus_tag="HS870_RS00015"
                     /old_locus_tag="D647_p17003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3856..>4284
                     /locus_tag="HS870_RS00040"
                     /pseudo=""
     CDS             3856..>4284
                     /locus_tag="HS870_RS00040"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4325..4822
                     /locus_tag="HS870_RS00025"
                     /old_locus_tag="D647_p17005"
     CDS             4325..4822
                     /locus_tag="HS870_RS00025"
                     /old_locus_tag="D647_p17005"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            4829..5044
                     /locus_tag="HS870_RS00030"
                     /old_locus_tag="D647_p17006"
     CDS             4829..5044
                     /locus_tag="HS870_RS00030"
                     /old_locus_tag="D647_p17006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          3047..3222
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.449"
                     /coverage="81.0"
                     /evalue="5.74e-59"
     Typing          complement(2566..3064)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.04"
                     /coverage="92.0"
                     /evalue="5.84e-177"
     Typing          3638..5160
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.803"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cacacagaga gtctttttta accgaaatca ccggctatgc agtgatgtga ataagtgatg
       61 catatgcgta aaataaatca ggtgcgtttc tggtggtttt cggtgtactt gccaccacct
      121 tttgcccgtc agccatcaga aacgccctga gtgcctccgg cgcggtgcgg gtaagagatg
      181 ttatgttcaa tcgaaatcag aaaaacgatc ggtaaagcga tcgtttttct gatagcatca
      241 tgttgatttt atataacgtg ggtgtaaaga atgactagac cgattgctgt tgatttattt
      301 gctggagcgg gtgggctgag tctcggtttc gagcaagctg gctttgatat aatggcatca
      361 gttgaaatag atccaataca ttgctctgct cacaaatata actttcctat gtgcaaaacg
      421 atatgtagaa gtgttgttga tgttaccgga gaatacgtta gggaagtttc aggtattggt
      481 gagaaagaga ttgatgtagt gatcggaggc gctccctgcc aaggcttttc attaatagga
      541 aaacgcgcgc ttgatgatga tagaaacaaa ctggtctatc actatgtacg gctggttctt
      601 gagctaaagc ctaaatactt tgtttttgaa aatgtaaaag ggttgacggt tggtaagcat
      661 aagcagtttt tagtagagat aatggatgca tttaaagatg gcgggtacga cgttgtaagc
      721 gattataaag tgctaaacgc agctgattat ggagttcctc aagatcgccg tcgtcttttc
      781 ttaatggggg gaaggaaagg aattgctctg ccatcatacc ctgagccatt atccattaga
      841 acaacggttg gggacgctat tggggatatt cctgatgcgg aaaaatatcc tgagctatgg
      901 ggtagagatt gggtttatgc taaatttggt gagcaatcac tatactcatc ctatttgcat
      961 ggcgtaggaa aagatcctag tgattttagc tacccaagga tttttaataa agacaaatta
     1021 acgtgttctt tactgacaga tcactctgac atttctagag ggcggtttgt tgaaactttg
     1081 cctgacacgg tagaaccaat cagcaggttt aaaaagctct cgatgaatgg gctatgtaat
     1141 actttaaggg caggaacagc aagtgacaga ggtgctttta catctcctcg tcctatacat
     1201 ccgatctatc ctagggtcat cacggtcaga gaggcagcaa gactgcattc atatcctgat
     1261 tggttcagat ttcatgtgac taagtggcat ggatttagac aggtcggtaa tagtgttcct
     1321 cctatgctag ctagaagtgt tggcttgcag attatgaagg ttcttggtgt tacgccaaaa
     1381 aaaccaacga agaaaatcaa actcggtgat gaaagtttgg ttgctatttc tatgtccgat
     1441 gctgcatcta tgtttggtgt gtcatcaaat gtaattgcta agagaataaa aaatagtaac
     1501 aaggaggttg tttgtgagta aggataaaaa agaaaggtcc aaaaatagat accatcagtt
     1561 ggtagaaaag atatttttcg ataaatatgt tgatggtgac ttagaggttt tgtttgaaag
     1621 gttagatata aacagtgctg caaaagattt aggtatagaa ctcccatcta atgttggtga
     1681 tgttatatat tcaatcagat ttagaacgcc tatgccagaa agtattctgt tgactcagcc
     1741 agaaggtaag gagtggatta ttgagctggc tggtaggtca aagtatcgat ttagattagt
     1801 aactattagt aggattttgc caaatcctaa tatggtggca ataaaaattc cagacaatac
     1861 cccggagata attacaggtt attctttaga cgacgagcag gcattgctgg cgaaggtgag
     1921 atataaccgg ctaatagata tatttctagg tataaccaca tattctttac agaatcattt
     1981 aagaactacg gtgaagggaa ttggtcaaat agagatcgat gaactttatg taggtataga
     2041 taaatacggt tgccattata taataccagt tcaagcaaaa ggtgggacag atcaaatatc
     2101 aatggtgcaa acatcgcaag atacagcgtg gtgtgaacaa aaatacccaa atgttagatg
     2161 tagagctatt tccgctcagt ttgtttcgag cgatttgatt gcactgtttg agctaaagat
     2221 agacgaagga caagtgaaat tgattgagga aaggcattac aaactggttc cggtaaaaga
     2281 gttagatagc tctgacatct caaactaccg catgtctgta taatttctga gaggatcttc
     2341 ttgagatcct ttcgttctag ccgtaatctc ctaagccgta aacaaaaaaa ccaccgctac
     2401 caacggtggt tcgtttgccg gaatttagag ctaccaactc tttcttccga aggtaactgg
     2461 ctttcacgga gcgcagatac caaaactgtc ctttcagtgt agccgtagtc aggccaccac
     2521 ttcaagaact ctgtagataa ctctactccg ctccgtgaat ccgattacca gtggctgctg
     2581 ccagtggcgc ttttgcatgt cttttcgggt tggactcaag acgatagtta ccggacaagg
     2641 cgcagcagtc ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct
     2701 acccggaact gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca
     2761 ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agctaccagg gggaaacgcc
     2821 tgggatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca gattttgtga
     2881 tgcttgtcag gggggcggag cctatggaaa aacggcaacg ccgcatcctt tttcctgact
     2941 ttcctgactt tcctgacttt cctgacttga gcgcgatctc gttatctcgt ccttcgtgag
     3001 ctggtaacgc ccgccgcagt ctcacggcag gagcgcagcg accgagtgag cgaggaggcg
     3061 tcatttcatc gttacctgca ttttgcactt acgccccgca gccgtatttt cccccttgcc
     3121 acatgaagca cttccccgaa tctccatctc gccgtaacat actaagccag tatacactcc
     3181 gctagcgcta cgtgactggt tcagggctgc gccccgaaac cccactgacg cgcctgcggc
     3241 ttgtccaacc ccgcgccgac cgggaaaggc tttcccgtcc gtcacggcgt tatcaggagg
     3301 cgattctggc agataaacgc aacaagatgc tcacgatgtg ggtgacggaa gacgaacacc
     3361 ggcggttgct tgagcggtgc gacggaaaac aactggcggc gtggatgcgc cagacctgcc
     3421 ttgatgagaa accgacacgc gccggcaggc ttccctcgat ttcgcccgcg ctgctgcgtc
     3481 agcttgccgg catgggcaat aacctgaacc agatcgcccg gaaagtgaac gccggtggtg
     3541 ccggtcatga ccgggtgcag atcgtggcag cgctgatggc gatcgatgcc ggtctggaac
     3601 ggttgcggca tgccgtactg gaaaaagggc cggacgatga ttgttaaatt ccatccccgg
     3661 ggtcgcggcg ggggcgccgg tcctgtggat tatcttctgg gtaaagaccg gcagcgtgaa
     3721 ggtgccagcg tcctgcaggg gaagccggaa gaagtccggg agctgattga tgcgtcgccg
     3781 tatgtgaaaa aatatacctc cggggtgctg tcattcgcgg aggcggacct gccgccgggt
     3841 cagcgtgaaa aactgatggc cagctttgag cgggttctga tgcccggact cgataaagac
     3901 cagtacagca ttctgtgggt ggaacacgca gacaagggtc gtcttgagct gaattttctg
     3961 atccccaata ccgaattact gacggggaaa cgcctccagc cgtattacga tcgtgccgac
     4021 cggccgcgca tcgatgcgtg gcagacagtg gtcaacggca ggctggggct acatgatccg
     4081 aacgacccgg agaaccggcg ggcgctggtc acgccttcag gactgccaga aacgaagcag
     4141 gaagccgcag agacgattac acggggtttg ctgatgttag cctcatcagg agagctgaaa
     4201 acgcgtgatg acgtcacagg ggcgctcaca gcggccggtt ttgaggtggt cagaaccaca
     4261 aaaaacagta tcagcattgc cgacccggac ggggggcgaa atatccgact gaggggagcc
     4321 atttatgagc agtcttttaa cggcctcgag ggacttcgag gagaaatcga aagcacagca
     4381 gcagcttacc gaagagaggc tgaaagccgc attcagcaag cacgagaacg ttgtcaaatc
     4441 ggaactgaac gcaagcgcga agagaatcaa cgacgccatc agcgcccacg aacaggggat
     4501 gaacgcggcg atgcagtcga accggctgag cgtgctgcga atggtcgggc ggacgtggct
     4561 gaccatcacc atggtgtcag ggctgctgtt cgccagcctg agcggggtgc tgtggtacca
     4621 ggggaaccgg atagcggcga acctggcgga aatcaggcag cagagagata cgctgtcgaa
     4681 actgcacatg cagacgtggg gcgtgaccta tctggagaac cgcaacggac ggtttctggt
     4741 gctgccggag gggatgaaag ccgaaacggg ccggacggtg gacaacaaga cgcggaacgc
     4801 ggtgaagctg gtacgggagt agagcagaat gacggaactg gaaaaacaat tgttgagcgc
     4861 gttagagcag ctacagcagg actactcgag aaggctggac gagtgggaga gcgccttcga
     4921 ggaatggcgg aagatgtctg gtcttatgca acgggagaac gcggcgctga gcgagcgcgt
     4981 gtcgggcttg agcaggcagg tgcagagttt gagcgagcag ctgcgccggt tatcgacagg
     5041 ctgaatgaga ttgagcaaca gcgatacggg caaacaacgg aaaaggaacc agagaagaca
     5101 gcagatctgg taccggaacg gcaacgcaca ttatctcatg gtgacaggga gtatggagac
     5161 tgataaacgc ccgtctcagg tggcctgagc gtgacgggga tcatggccat tttctgcgat
     5221 ttcag
//
