LOCUS       NC_019986               3510 bp    DNA     circular CON 25-SEP-2023
DEFINITION  Enterobacter cloacae plasmid pB1023, complete sequence.
ACCESSION   NC_019986
VERSION     NC_019986.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226180
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   San Millan,A., Santos-Lopez,A. and Gonzalez-Zorn,B.
  TITLE     ColE1 plasmids and antibiotic resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   San Millan,A., Santos-Lopez,A. and Gonzalez-Zorn,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-DEC-2011) Sanidad Animal, Universidad Complutense de
            Madrid, Avda. Puerta de Hierro s/n, Madrid, Madrid 28040, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/25/2023 00:40:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5
            CDSs (total)                      :: 5
            Genes (coding)                    :: 4
            CDSs (with protein)               :: 4
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JQ319770.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="BB1092"
                     /db_xref="taxon:550"
                     /plasmid="pB1023"
     gene            497..838
                     /locus_tag="HS874_RS00005"
                     /old_locus_tag="D727_p2001"
     CDS             497..838
                     /locus_tag="HS874_RS00005"
                     /old_locus_tag="D727_p2001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1047..>1475
                     /locus_tag="HS874_RS00030"
                     /pseudo=""
     CDS             1047..>1475
                     /locus_tag="HS874_RS00030"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1516..2013
                     /locus_tag="HS874_RS00015"
                     /old_locus_tag="D727_p2003"
     CDS             1516..2013
                     /locus_tag="HS874_RS00015"
                     /old_locus_tag="D727_p2003"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            2020..2235
                     /locus_tag="HS874_RS00020"
                     /old_locus_tag="D727_p2004"
     CDS             2020..2235
                     /locus_tag="HS874_RS00020"
                     /old_locus_tag="D727_p2004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2606..2812
                     /locus_tag="HS874_RS00025"
     CDS             2606..2812
                     /locus_tag="HS874_RS00025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064408406.1"
                     /translation="MLITGVRVMLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYM
                     ENPTSANYKIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     Typing          239..412
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(2..130)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.366"
                     /coverage="85.0"
                     /evalue="3.47e-44"
     Typing          complement(3056..3510)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.474"
                     /coverage="100.0"
                     /evalue="2.95e-130"
     Typing          829..2351
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gggagccgcc aggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg
       61 atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt
      121 cgccgcggcc ctatcgcttc cctgttaagt atcttcctgg catcttccag gaaatctccg
      181 ccccgttcgt aagccatttc cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg
      241 agcgaggaag cggaatatat cctgtatcac atattctgct gacgcgccgg tgccgccttt
      301 tttctcctgc cacatgaagc acttcactga catcctcatc cgtgccaaca tagtaagcca
      361 gtatacactc cgctagcgct acgtgactgg ttcagggctg cgccccgaaa ccccactgac
      421 gcgcctgcgg cttgtccaac cccgcgccga ccgggaaagg ctttcccgtc cgtcacggcg
      481 ttatcaggag gcgattctgg cagataaacg caacaagatg ctcacgatgt gggtgacgga
      541 agacgaacac cggcggttgc ttgagcggtg cgacggaaaa caactggcgg cgtggatgcg
      601 ccagacctgc cttgatgaga aaccgacacg cgccggcagg cttccctcga tttcgcccgc
      661 gctgctgcgt cagcttgccg gcatgggcaa taacctgaac cagatcgccc ggaaagtgaa
      721 cgccggtggt gccggtcatg accgggtgca gatcgtggca gcgctgatgg cgatcgatgc
      781 cggtctggaa cggttgcggc atgccgtact ggaaaaaggg ccggacgatg attgttaaat
      841 tccatccccg gggtcgcggc gggggcgccg gtcctgtgga ttatcttctg ggtaaagacc
      901 ggcagcgtga aggtgccagc gtcctgcagg ggaagccgga agaagtccgg gagctgattg
      961 atgcgtcgcc gtatgtgaaa aaatatacct ccggggtgct gtcattcgcg gaggcggacc
     1021 tgccgccggg tcagcgtgaa aaactgatgg ccagctttga gcgggttctg atgcccggac
     1081 tcgataaaga ccagtacagc attctgtggg tggaacacgc agacaagggt cgtcttgagc
     1141 tgaattttct gatccccaat accgaattac tgacggggaa acgcctccag ccgtattacg
     1201 atcgtgccga ccggccgcgc atcgatgcgt ggcagacagt ggtcaacggc aggctggggc
     1261 tacatgatcc gaacgacccg gagaaccggc gggcgctggt cacgccttca ggactgccag
     1321 aaacgaagca ggaagccgca gagacgatta cacggggttt gctgatgtta gcctcatcag
     1381 gagagctgaa aacgcgtgat gacgtcacag gggcgctcac agcggccggt tttgaggtgg
     1441 tcagaaccac aaaaaacagt atcagcattg ccgacccgga cggggggcga aatatccgac
     1501 tgaggggagc catttatgag cagtctttta acggcctcga gggacttcga ggagaaatcg
     1561 aaagcacagc agcagcttac cgaagagagg ctgaaagccg cattcagcaa gcacgagaac
     1621 gttgtcaaat cggaactgaa cgcaagcgcg aagagaatca acgacgccat cagcgcccac
     1681 gaacagggga tgaacgcggc gatgcagtcg aaccggctga gcgtgctgcg aatggtcggg
     1741 cggacgtggc tgaccatcac catggtgtca gggctgctgt tcgccagcct gagcggggtg
     1801 ctgtggtacc aggggaaccg gatagcggcg aacctggcgg aaatcaggca gcagagagat
     1861 acgctgtcga aactgcacat gcagacgtgg ggcgtgacct atctggagaa ccgcaacgga
     1921 cggtttctgg tgctgccgga ggggatgaaa gccgaaacgg gccggacggt ggacaacaag
     1981 acgcggaacg cggtgaagct ggtacgggag tagagcagaa tgacggaact ggaaaaacaa
     2041 ttgctgagcg cgttagagca gctacagcag gactactcga gaaggctgga cgagtgggag
     2101 agcgccttcg aggaatggcg gaagatgtct ggtcttatgc aacgggagaa cgcggcgctg
     2161 agcgagcgcg tgtcgggctt gagcaggcag gtgcagagtt tgagcgagca gctgcgccgg
     2221 ttatcgacag gctgaatgag attgagcaac agcgatacgg gcaaacaacg gaaaaggaac
     2281 cagagaagac agcagatctg gtaccggaac ggcaacgcac attatctcat ggtgacaggg
     2341 agtatggaga ctgataaacg cccgtctcag gtggcctgag cgtgacgggg atcatggcca
     2401 ttttctgcga tttcagcaca cagagagtct tttttaaccg aaatcaccgg ctatgcagtg
     2461 atgtgaataa gtgatgcata tgcgtaaaat aaatcaggtg cgtttctggt ggttttcggt
     2521 gtacttgcca ccaccttttg cccgtcagcc atcagaaacg ccctgagtgc ctccggcgcg
     2581 gtgcgggtaa gagatgttat gtttaatgtt gataacagga gtaagagtga tgcttacatt
     2641 tattggtgta gtggtagtcg ccgttattgc atttaaatta tttatgcttt tttttagttc
     2701 atccgaatct gccgtcgaac gtgctacccg taaatatatg gagaacccta cgagtgctaa
     2761 ctataaaata atggtggcta cacgcatgag aaatgagaag aaaagagggt gatatatgtt
     2821 tgagtttatt ggttttttat ttttactttt ggtgtgttat gtctttatac aaatggttgc
     2881 tgtcagggtg tttcctgaat atggagtacg gaaagcggag gaacgatacc gtcgaaatcc
     2941 atcagcagaa aatgaggatc tgttatggag agctagaagt cgtataacta aacgcaagta
     3001 atgtttccta agtgtgttga taggcaaaca tggtgggagt tactactctc ggagttaatg
     3061 atcttcttgt gatcctgtcg tttcagtcgt aatctcttgc tctgaaaacg aaaaaaccac
     3121 ctggggaggt ggtttgatcg aaggttaagt cagttgggga actgcttaac caggtaactg
     3181 ggtatacgag accgcagata ccaaaactgt tctttcagtg tagccgcagt caggcccata
     3241 cttcaagacc tctgaatctc tctctcgtat atcccagtta ccagtggctg ctgccagtgg
     3301 cgctttgtcg tgctttctcg ggttggactc aagatgatag ttaccgggta aggcgcagca
     3361 gtcggactga acggggggtt tgtgcataca gtccagcttg gagcgaactg cctacccgga
     3421 actgagtgtc aggcgtggaa tgagataaac gcggccataa cagcggaatg acaccggtaa
     3481 accgaaaggc aggaacagga gagcgcacga
//
