LOCUS       NC_019982               6292 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli plasmid pEC159, complete sequence.
ACCESSION   NC_019982
VERSION     NC_019982.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226115
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Kaczorowska,A.-K. and Kaczorowski,T.
  TITLE     A natural variant of Escherichia coli plasmid pLG13 carrying genes
            encoding the EcoRV restriction-modification system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Kaczorowska,A.-K. and Kaczorowski,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2011) Microbiology, University of Gdansk, Kladki
            24, Gdansk 80-822, Poland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009957985.1-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 03:14:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10
            CDSs (total)                      :: 10
            Genes (coding)                    :: 9
            CDSs (with protein)               :: 9
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JN692546.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /plasmid="pEC159"
     gene            complement(206..337)
                     /locus_tag="HS811_RS00050"
     CDS             complement(206..337)
                     /locus_tag="HS811_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071598030.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGRGCTRNC"
                     /name="replication initiation protein"
     gene            complement(1005..1196)
                     /locus_tag="HS811_RS00005"
                     /old_locus_tag="D616_p95001"
     CDS             complement(1005..1196)
                     /locus_tag="HS811_RS00005"
                     /old_locus_tag="D616_p95001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1261..1584
                     /locus_tag="HS811_RS00010"
                     /old_locus_tag="D616_p95002"
     CDS             1261..1584
                     /locus_tag="HS811_RS00010"
                     /old_locus_tag="D616_p95002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_069354221.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSCDRVQVVAALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1793..>2224
                     /locus_tag="HS811_RS00045"
                     /pseudo=""
     CDS             1793..>2224
                     /locus_tag="HS811_RS00045"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2262..2780
                     /locus_tag="HS811_RS00020"
                     /old_locus_tag="D616_p95004"
     CDS             2262..2780
                     /locus_tag="HS811_RS00020"
                     /old_locus_tag="D616_p95004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328227.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            2787..3020
                     /locus_tag="HS811_RS00025"
     CDS             2787..3020
                     /locus_tag="HS811_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_011117658.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSRTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3051..3212
                     /locus_tag="HS811_RS00055"
     CDS             3051..3212
                     /locus_tag="HS811_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_073481910.1"
                     /translation="MRYPWSVSASWKYRNAHWMVLRWDGETMKNGRLSGLRQANVVCTI
                     WLHRYSCG"
                     /name="mobilization protein"
     gene            complement(4292..5029)
                     /locus_tag="HS811_RS00030"
                     /old_locus_tag="D616_p95005"
     CDS             complement(4292..5029)
                     /locus_tag="HS811_RS00030"
                     /old_locus_tag="D616_p95005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013934312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme EcoRV"
                     /protein_id="WP_011117659.1"
                     /translation="MSLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFE
                     LFSRPIINKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENEKIK
                     FTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVATRKSSLKTYNINELNEIPKPY
                     KGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKGIFDSEDEFLDYWRNYERT
                     SQLRNDKYNNISEYRNWIYRGRK"
                     /name="type-2 restriction enzyme EcoRV"
     gene            complement(5013..5312)
                     /locus_tag="HS811_RS00035"
                     /old_locus_tag="D616_p95006"
     CDS             complement(5013..5312)
                     /locus_tag="HS811_RS00035"
                     /old_locus_tag="D616_p95006"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_015328228.1"
                     /translation="MPKKPILGYPILGCIIYKKNDGKSMSVREIIKKNRVICKLSQGEV
                     AKALGKPQSYISKIEQGERRVDVDEFIAICFAIGIDPINTLKEVIMEKKHESSF"
                     /name="helix-turn-helix transcriptional regulator"
     gene            5336..6232
                     /locus_tag="HS811_RS00040"
                     /old_locus_tag="D616_p95007"
     CDS             5336..6232
                     /locus_tag="HS811_RS00040"
                     /old_locus_tag="D616_p95007"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="WP_001570854.1"
                     /translation="MKDKVFVPPIKSQGIKTKLVPWIKRIVPKNFNGVWVEPFMGTGVV
                     AFNVAPKDALLCDTNPHLISFYNALKNKDITGDLVKDFLYREGEKLLLSNGEYYYEVRE
                     RFNNYKEPLDFLFLNRSCFNGMIRFNSKGGFNVPFCKKPNRFAQAYITKISNQVDRISE
                     IISKGNYTFLCQSFEKTIGMVNRDDVVYCDPPYIGRHVDYFNSWGERDERLLFETLSSL
                     NATFITSTWHHNDYRENKYVRDLWSSFRILTKEHFYHVGASEKNRSPMVEALITNIAKD
                     IIDHIEKSSGDILVIEE"
                     /name="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
     Typing          834..921
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(266..810)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.264"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1575..3124
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.038"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agatctcaag atcctcttga gatccttttt tttgcgcgta atctgctgtc tgtagacgaa
       61 aaaaccaccc tggcaggtgg tttttcgaag gttaaataat cctggcagat tatttaaccg
      121 tggtaacagg gtgtacaaga ccgctgccac caaatctgtc ctttcagtgt agccgcagtt
      181 ggtccttcac ttcaagaact acgtatcagc aatttcttgt acatcctcta ccagtggctg
      241 ctgccagtgg cgttaaggcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      361 cctacaccga actgagatac caacagcgtg agctatgaga aagcgccacg cttcccgaag
      421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgcctcct
      601 gcgcgccctt tttctgggtc tttgtctttt gctcacatgt tctttcctgc gttatcccct
      661 gattctgtgg ataaccgttt accgcctttg agtgcgctga caccgctcgc cgcagtcgaa
      721 cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga gcgcctggac gtgcattttc
      781 tccttacgca tctgtgcggc atttcacacc cggcatggcg tacttttcat acaatccgca
      841 ctgatgccgc atggttaagc cagtatacac tccgctatcg ctacgtgact gggtcagggc
      901 tgcgccccga cacccgctaa aacctgctga cgcgccctga cgggcttgtc tgctcccgga
      961 gacgctcaca gacaagctgt gagcgtctcc gggagttgca tgtgtcagag gttttcaccg
     1021 tcatccccga aacgtgcgag gcagctgcgg taaagctcat cggcgtggtc gtgaagcgat
     1081 tcacagatgt ctgcctgttc atctgcgtcc agttcgttga gcttctccag cagcgttaat
     1141 gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt agtcactgat
     1201 gcctccgtgt aaggggggat ttctgttcat ggggtaatga taccgatgaa acgcgagagg
     1261 atgctcacga tacgggttac tgatgatgaa catgcacggt tactggaacg ctgtgagggt
     1321 aaacagctgg cggtatggat gcggcgggtc tgcctgggtg agccggttgc ccgttccggg
     1381 aagctgccga cactggcacc gccgttactg cgccagctgg ctgccatcgg gaataacctg
     1441 aatcagacgg cgaggaaggt gaacagcggg cagtggtctt cctgtgaccg ggttcaggtg
     1501 gtggccgcac tgatggccat cgagagagag ttgcgcagct tacggcaggt cgtcagggaa
     1561 cagggggcgc gggatgatag ttaaatttca tgccagggga aaaggtggtg gcagtggtcc
     1621 ggttgattac ctgctgggga gggagcgtaa ccgggaaggg gcaacggtgc ttcagggtaa
     1681 tccggaagaa gtccgggaac tcatcgatgc cacgccattt gcgaagaaat acacgtccgg
     1741 tgttctgtcg ttcgcggaga aggaactgcc gccgggagga cgtgaaaaag tgatggcgag
     1801 ctttgagcgc gtactgatgc caggtctcga aaagaatcag tacagcatcc tgtgggtgga
     1861 gcaccaggac aagggacggc ttgagctgaa ttttatcatt ccgaacatgg agctacagac
     1921 cggaaaacgt ctccagccgt actacgaccg ggcagacagg ccgagaattg atgcctggca
     1981 gacgctggtg aatcaccatt acgggctgca tgaccccaac gccccggaga accgccggat
     2041 actgactctc tctgataacc tgccggaaac gaaacaggcg cttgctgaga gcgtcacacg
     2101 cgggatagac gctctttatc atgtagggga gataaaaggc cgtcaggatg tgattcaggc
     2161 gctcacagag gcggggcttg aagtggtcag ggtgacgcga agcagcatca gcattgcaga
     2221 cccgaacggc gggaagaata tcaggctgaa aggagcgttt tatgagcaat cttttacaga
     2281 cgggcgcgga gttcgagaaa aagctgaaag agagagccga atctacagag aaaatgctga
     2341 acgacgagtt caggaagctc ggcgagtctg tcagcgaggc tgtgacctca aacgagacga
     2401 aaatcagaga cgctatagcc ctgttcacag cctcgacaga gaaatcgctg gaaaaacaca
     2461 gggaaggggt gaaagaggcg atgatgcagc acaggaagga cgtgttaaag ctggcaggga
     2521 atacgggcat gatgttactg gggatagtct ttctcctgtt taccgcgagt ggcgggacgc
     2581 tctggtatct tggcgggagg atacaggcga acctggaaga aatcaggaag caggaagaga
     2641 cattgcagaa actgaacgcg aagacatggg gcgtggagtt tgtgcaggac gggaacagga
     2701 aatttctggt ccttccgtac gggaaatcag cagaggtgat tccctttcag gggaaagagt
     2761 gggtacatct gaaggagtga cacagaatga cagagctgga aacacatttg ctgaacgcct
     2821 tagagcagct gcaacaggac tatatgcagc ggctgagcga atgggagagc gccttcgtgg
     2881 aattgcagaa gatgttttcg cgtacgcaac gggacaacgc gatgctgaac gagcgggtca
     2941 tgcagttgag tcagcaggtg cagcacttga gcgagcagac agaacgcttg agccagttat
     3001 acagcgagaa ctggagataa gagaggaacg actgatacag gagcgcgaac atgcgttatc
     3061 cctggagcgt gagcgccagc tggaaataca ggaacgcaca ctggatggtc cttcgctggg
     3121 atggtgaaac tatgaaaaat ggcaggttaa gtggattaag acaggctaat gtggtctgta
     3181 ccatctggtt gcataggtat tcatgcggtt aaaatttatc aggcgcgatt gcggcagttt
     3241 ttcgggtggt ttgttgccat ttttacctgt ctgctgctgt gatcgcgctg aacgcgtttt
     3301 ggcggtgcgt acaatttgta attatgttaa atataataga taagctcagg gtgggggcct
     3361 atggcttata tttgactata aagctattta tcatttattt tccatctcga tgcattatta
     3421 ataaatttct tttattacaa taaaagacga ggatatgatt tacttttaga gtatgtgatt
     3481 tgataggcaa tgctgttgat atatagcatt taatgcttat cctgtattgg ggttttaaat
     3541 tatacattat ttaaatacta aactctctaa gagtcggagg agaagtgatg ttgtaaataa
     3601 gaatacatga ggactgatgc cagtgattct aatattaaac tatatcaaga aatttcctca
     3661 actgatgtat tgattttttg taagggcgcg tttatgtaaa gaggtgtatg aataacaaga
     3721 agtaaataaa cgtcattata ttacatataa tggttttcta ttttatttta ttattgtgtt
     3781 cgttgcaata aaagaagctg tttatataaa aatatgcgtg tggtttatga taagctaaaa
     3841 atactttttg taattaacaa aaatgtgttg atttttatta agaatcaagt tacaacggac
     3901 atgggtgcgt ttctagtcgt ttatcattgt aaccggtctc aatgtgagaa gcgcacccaa
     3961 ttcttatttt ccgcaatgta tagacataca gaaaaaattc tctctcattg gtgttgaatt
     4021 aaacatcatt caagtgatat gtatatttat tgtgcgaaat gcagagggaa aatatagaga
     4081 ggcatatgtt tttatgtctt tatgttgttg tggtgagagt gtaagggtgc atcatgtcat
     4141 ctttgaatgt taagtgtgat gtgccggagg agggaatagt ctgatgctaa attttttctt
     4201 ttagtatccc tccctccccc tctttctctc atgagtcaga gaggttttat tctctgttag
     4261 agggggggga tataaacata taagctattg cttattttct tcctcggtat atccagtttc
     4321 tgtattcgct tatattatta tacttgtcat ttcttaattg actggttctt tcataatttc
     4381 tccaatagtc taaaaactca tcctctgagt caaatattcc ttttccttct acaaagtctt
     4441 tatagtgggc atgaatgcta cctatatttg ttgtgtttcc agatcctgcc aaatctccag
     4501 caataaccca tttatcttgt aagaaaacct ttacgccttt gtatggttta gggatttcat
     4561 tgagttcatt tatattatat gtttttaaag atgattttct tgtagcaact cttgtatata
     4621 catatccgat tatccaatgg gcgatatatt ggtcaaatgg ataaacaata ttttttgtgt
     4681 tgtttcgtat aaagctggta tacccaccaa gagtgaactt gattttttcg ttttctttgt
     4741 ttgtatatgt tgtttttata tctattgcaa tttttttatt tggttcgctt ggtttgtaaa
     4801 gagtaaagtc aggataatga ttttgttgtt taggttcttc tacaatatac ccatgttttt
     4861 ctgctatttt atttattatt ggtcttgaga ataactcaaa tattgtgctt agaacttttg
     4921 tgtcacttcc caatgggtat atttttcctt ctgcagatat tattccgcat acatcatatt
     4981 tttgattttc atcatatagt gcattaatta aatcagaacg aagactcatg ttttttttcc
     5041 attattactt ctttaagtgt atttattggg tcaattccta ttgcaaaaca gatggctata
     5101 aattcgtcaa cgtcaacgcg tctttctcct tgttctattt tagatatgta ggattgtggc
     5161 tttccaagtg ccttggcaac ctctccttga gagagtttac aaattactct attttttttt
     5221 atgatttctc ttacagacat agatttacca tcattttttt tatatattat gcaccccaaa
     5281 atgggataac ccaaaatggg tttctttggc atatttttta caaatggttc ttattatgaa
     5341 agataaagtt tttgttccgc ctataaaatc gcaagggatt aagactaaac tggttccgtg
     5401 gataaagaga attgttccta aaaactttaa tggggtatgg gtcgagccgt ttatggggac
     5461 tggagttgtt gcctttaacg ttgctcctaa agatgcatta ttatgtgata caaaccctca
     5521 tttgatttct ttttataatg cattgaaaaa taaagatata acaggggatc ttgttaagga
     5581 ttttttatat cgtgaaggtg aaaaactctt attaagtaat ggtgagtatt attatgaagt
     5641 cagggaaagg tttaataatt acaaggaacc gttagatttt ttattcttaa ataggtcttg
     5701 ttttaatggt atgattaggt ttaatagtaa aggagggttt aatgttcctt tttgtaaaaa
     5761 accaaacaga tttgcacaag catatattac caagatttcg aatcaagttg ataggatttc
     5821 tgaaataata tcaaaaggga actatacatt cttgtgtcag tcatttgaaa aaactattgg
     5881 catggtaaat agggatgatg ttgtttattg tgatcctcca tatattggta ggcatgttga
     5941 ttattttaat tcatggggag agcgtgacga gcgtttatta tttgaaacgt tatcatcttt
     6001 gaatgctaca tttataacgt ctacgtggca tcataatgat tatagggaaa ataagtacgt
     6061 tcgtgattta tggtcatctt ttagaatatt gacaaaagaa catttttatc atgttggagc
     6121 gtccgaaaaa aacagatccc cgatggtaga ggcgctaatt acaaatatag ccaaggatat
     6181 tattgatcac attgaaaaaa gtagtgggga tattttggta attgaagagt gatggagatg
     6241 aggacataaa gtgaccactc tgtacgttta tcgtgagagg ggatatgtaa aa
//
