LOCUS       NC_019359              10879 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Acinetobacter baumannii plasmid pAB120, complete sequence.
ACCESSION   NC_019359
VERSION     NC_019359.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226317
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Povilonis,J., Seputiene,V., Krasauskas,R., Juskaite,R.,
            Miskinyte,M., Suziedelis,K. and Suziedeliene,E.
  TITLE     Spread of carbapenem-resistant Acinetobacter baumannii carrying a
            plasmid with two genes encoding OXA-72 carbapenemase in Lithuanian
            hospitals
  JOURNAL   J Antimicrob Chemother 68 (5), 1000-1006 (2013)
   PUBMED   23258313
REFERENCE   2  (bases 1 to 10879)
  AUTHORS   Povilonis,J., Seputiene,V., Jurenaite,M., Juskaite,R., Krasauskas,R.
            and Suziedeliene,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2012) Department of Biochemistry and Biophysics,
            Vilnius University, Faculty of Natural Sciences, M. K. Ciurlionio
            21, Vilnius LT-03101, Lithuania
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 11/28/2023 05:09:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 14
            CDSs (total)                 :: 14
            Genes (coding)               :: 14
            CDSs (with protein)          :: 14
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JX069966.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10879
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="K60"
                     /isolation_source="respiratory tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pAB120"
                     /geo_loc_name="Lithuania"
                     /collection_date="2010"
     gene            322..1272
                     /gene="repM"
                     /locus_tag="HPE87_RS00005"
                     /old_locus_tag="pAB120_01"
     CDS             322..1272
                     /gene="repM"
                     /locus_tag="HPE87_RS00005"
                     /old_locus_tag="pAB120_01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            1265..1840
                     /locus_tag="HPE87_RS00010"
                     /old_locus_tag="pAB120_02"
     CDS             1265..1840
                     /locus_tag="HPE87_RS00010"
                     /old_locus_tag="pAB120_02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            1860..2003
                     /locus_tag="HPE87_RS00015"
     CDS             1860..2003
                     /locus_tag="HPE87_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(2185..3012)
                     /locus_tag="HPE87_RS00020"
                     /old_locus_tag="pAB120_03"
     CDS             complement(2185..3012)
                     /locus_tag="HPE87_RS00020"
                     /old_locus_tag="pAB120_03"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            3621..3977
                     /locus_tag="HPE87_RS00025"
                     /old_locus_tag="pAB120_04"
     CDS             3621..3977
                     /locus_tag="HPE87_RS00025"
                     /old_locus_tag="pAB120_04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269902.1"
                     /translation="MWTVITTDLFNEWLAQQDESTQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKGGKKRFYKEMLDIADQQ
                     YELHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3970..4272
                     /locus_tag="HPE87_RS00030"
                     /old_locus_tag="pAB120_05"
     CDS             3970..4272
                     /locus_tag="HPE87_RS00030"
                     /old_locus_tag="pAB120_05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001140622.1"
                     /translation="MAKTLQELLASRSPESQARIQQMADELLLETQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            4265..4474
                     /locus_tag="HPE87_RS00035"
                     /old_locus_tag="pAB120_06"
     CDS             4265..4474
                     /locus_tag="HPE87_RS00035"
                     /old_locus_tag="pAB120_06"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069473.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCDYFS
                     NCKRSSYYIDIFDDAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(4800..5261)
                     /locus_tag="HPE87_RS00040"
                     /old_locus_tag="pAB120_07"
     CDS             complement(4800..5261)
                     /locus_tag="HPE87_RS00040"
                     /old_locus_tag="pAB120_07"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(5567..7978)
                     /gene="znuD2"
                     /locus_tag="HPE87_RS00045"
                     /old_locus_tag="pAB120_08"
     CDS             complement(5567..7978)
                     /gene="znuD2"
                     /locus_tag="HPE87_RS00045"
                     /old_locus_tag="pAB120_08"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            complement(8106..8420)
                     /locus_tag="HPE87_RS00050"
                     /old_locus_tag="pAB120_09"
     CDS             complement(8106..8420)
                     /locus_tag="HPE87_RS00050"
                     /old_locus_tag="pAB120_09"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(8407..8694)
                     /locus_tag="HPE87_RS00055"
                     /old_locus_tag="pAB120_10"
     CDS             complement(8407..8694)
                     /locus_tag="HPE87_RS00055"
                     /old_locus_tag="pAB120_10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            9003..9830
                     /locus_tag="HPE87_RS00060"
                     /old_locus_tag="pAB120_11"
     CDS             9003..9830
                     /locus_tag="HPE87_RS00060"
                     /old_locus_tag="pAB120_11"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            complement(9874..10065)
                     /locus_tag="HPE87_RS00065"
                     /old_locus_tag="pAB120_12"
     CDS             complement(9874..10065)
                     /locus_tag="HPE87_RS00065"
                     /old_locus_tag="pAB120_12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000032259.1"
                     /translation="MSKELVNLANIVPFPKSCGFCESKENVQLFAGLMLCSSCQENIRT
                     TNPGMFTANKQIEKKAQD"
                     /name="hypothetical protein"
     gene            complement(10299..10517)
                     /locus_tag="HPE87_RS00070"
                     /old_locus_tag="pAB120_13"
     CDS             complement(10299..10517)
                     /locus_tag="HPE87_RS00070"
                     /old_locus_tag="pAB120_13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002031357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015062952.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLKMMTFKALFHKVFGCFYRF"
                     /name="hypothetical protein"
     ARG             complement(2189..3012)
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             9004..9827
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          323..1272
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtcacgccag tattaaccaa aaaattgccc caacgaactg agcgaaagcg aagttcaata
       61 gagtttgagc gaagcgaaaa ccaagggcaa tttttcattc cctgggcttt taattatttt
      121 aaagttttta aatactttta aatggcttga aagccttatg aataaagggt tttagctctt
      181 atatgtccac gtttaccttg caatatgtcc acgtttacct tgcaatatgt ccacgtttac
      241 cttgcaatat gtccacgttt accttgcatt agtacacaaa taatattaac gtgtacttat
      301 acacaataaa aaatagtggc tatgagagat ttagttgtaa aggacaatgc cttaatcaac
      361 gcaagctata acttagactt agtagaacaa cgtttaattt tattggctat tgttgaagca
      421 agggaaagtg ggaaagggat taatgcaaat gatccattag aagttcatgc agatagttat
      481 atcaatcaat ttggtgtaca ccgtaataca gcttatcaag ccttaaaaga tgcttgtaag
      541 gatttattcg cgcgtcaatt tagctatcaa gagaaaaaag ctaatgggaa tatccgaaat
      601 gttatgagtc gttgggtatc tcaaattgct tataacgaca atgaagcaac tgtagactta
      661 atatttgcac ctgctgttgt tcccttcata acccgactgg aagaacaatt tactaaatat
      721 gaattacagc aagttagtag tcttagtagc gcttatgcca ttcgcttata tgagcttcta
      781 attcagtggc gaagcactgg taaaacccca accatagaac tacaagaatt tagaaagaag
      841 ttaggcgttc ttgataatga atatttacgg atggctcatt taaaagagcg tgttttagag
      901 ctttcaatta aacaaataaa tgagcatacg gatataactg taaaatatga acagcataaa
      961 agaggacgtt ctatttcagg attttctttt acctttaaac agaagaagaa ggatagccca
     1021 tcaatagaaa gagatccgaa cactttggag cttttttcaa agatgaccga tgctcaacgg
     1081 catatgtttg caaataaact ttcagaactc cctgaaatgg gtcgctattc acaaggaaca
     1141 gaaagctacc aacagtttgc tgtacgtatt gctgagatgc tacaagatcc cgctcaattc
     1201 aaagagctat acccatacct aaaaaaagtg ggatacatgc catcaaataa aaaggacacc
     1261 gtaaatggct aagttatcac taagtgaagt atctaaaaaa tttcatgtgg atagatcaac
     1321 catttacaga gctgtacgta atggacgttt atcacgctcc agtgatggac aattcgatct
     1381 agcagaggtc atacgatgct ttggagaacc tgagcaaaca tctcaaaaaa ttgaatcatc
     1441 taagcaagaa ggtgatgaat ctacaaaaaa acttattgcc catttagaaa atgaagtcaa
     1501 aaaataccaa gaacgtgaag aacggttaat gcaacaaatt gaccgtatgc aaacactcat
     1561 tgagctgaaa agtgttgcac ctgccacagc agcaccacac caagatgcta cggcatgcga
     1621 caccaagatg ccacagcatg cgacaacaca acaagacact gataacaaaa agaataatga
     1681 attaaatatt gcagaaaatg tagcagtgcc acagcaagaa actacggcat accacaccca
     1741 aacgctacag catgccacgt tgcaaagtgt ggcagtgcca caacacaaaa aacgtggttt
     1801 atttggccgt gtgctgaatg ccgtctttga taatgactga gcaataaggg tgaaagatca
     1861 tgccgaaact gaaagacatt gccctgggaa ttattgtagc tccgctgctg atcccgatca
     1921 tgctgattgc atcgtaccag gataaaaagg cgctgaagaa agagctggat gaatgccaaa
     1981 aagaaaaaga ccaggcatcc tgattattca attgagctaa tcaaaagtgt ggaacattga
     2041 tgaaataata gcgtggaacg tgaaaagtca taaaaaaagc ccgaacaggg atgctcggct
     2101 ataaactaga aactacagcg ttgaattata aggataatta tacagcattt cgtataaggt
     2161 gtattatgtt aattttagaa attattaaat gattccaaga ttttctagcg acttataagt
     2221 aatttcatta cgaatagaac cagacattcc ttctttcatt tctaagttga gcgaaaaggg
     2281 gatttttttt ccattagctt gctccaccca accagtcaac caacctacct gtggagtaac
     2341 atccattccc catccacttt ttgcataaat cttactacca tttacttctt taattagaag
     2401 cattttttta acttcttctt gagtttctaa tttaaaaggt aatcggttat gtgcaaggtc
     2461 atcggcaaaa ttaacttctt gtactggtgt aattttaagg gggccaacta accaaaaatt
     2521 atcgacctgt gttccaatat ttgtatttcc aaaattaacc cgctttactt ctttctgcat
     2581 tagctctagg ccagtccgtc ttgcaagctc ttgatatact ggaactgctg acaatgccat
     2641 tgcctcacct aaagtcatat ctttctccca cataggataa gttctttttt taccatccca
     2701 tttgaaaatc tcatttgttg ttgctttatg attttctagc ccgattaaag catttagcat
     2761 cttaaatgtt gatgcaggga catattcttt atttgctcgt gcaagagcat taccataggt
     2821 gctaagattt ttaccctctt taataataat tacaccctgt gtttgagctt catcaaaata
     2881 gcttttaata gctttttcat gttgctgaga agaaatatga aaattatctt cagatttagt
     2941 tttaatagat gaacatgcac tgagagaaac tagaatagaa atgctgaata taggaagtat
     3001 aaattttttc attacaaatt catgttaggg gaaattttgg ggcttagagc ataatatttt
     3061 tattaaaaaa gttaaaactc ttttaattta acataatggg cgttatgcga aatcaattta
     3121 aattatttta attaaatcaa atatttatgt attctacata acgccaaaaa ataccagaat
     3181 gccgactcgg aaacaagttg ggtatttttt aagcttcttt gtcactttta gccaataaaa
     3241 tagattaaaa aatattcaaa aattcagttt tctttgattt ctcaacagtc agcattttga
     3301 aataatccac aaaatttgcg gataaatctt gggataaata tacgccaatt atgtacgcct
     3361 aagcgcacac cgattgaggg tattaaccaa tagttagact cagcttaatt ttctatcatg
     3421 gacacataga gagcaggatg ctccagcaca caaaacaact atcataagtg agcgcactag
     3481 gatcgtgaag caaagtagaa gttacacttg agatgcaaaa acgaaaaagc cagacaggat
     3541 gtcgggcttt tttattgtca tttgaataat aattcaatta agcataaaat atagactgta
     3601 atctatatta tggttgtaat atgtggacag tcattacaac ggatctgttt aacgaatggc
     3661 ttgcacagca agatgaatct acacaagaga aagtactcgc agcgttggtc gttctacagc
     3721 aacagggacc aagtttaggt cgtcctttag tagataccgt ctatgactcc aaatttacca
     3781 atatgaaaga gctacgtgtt cagcatcgag gtaaaccact gagagccttt ttcgcatttg
     3841 atcctttacg acaagctatt gtgctgtgta tcggtgataa aggcggtaaa aagcgttttt
     3901 ataaagagat gttagatatt gctgatcagc aatacgaact tcatctctca actttaggag
     3961 atcaatctaa tggctaagac cctacaagaa ttattggcta gtcgctctcc agagagtcag
     4021 gctcgtattc aacaaatggc tgatgaatta ctgttggaaa ctcagcttca ccttattcgt
     4081 gaagagttag agatttctca aaaagaactg gctgcaaccc ttggaatcaa acagccgtca
     4141 ttgtcagcaa tcgaaaatcg tggtcatgat cttaaaattt caaccatgaa aaagtatgtt
     4201 gaagcaatgg gcggaaaact tcgtattgat gtagagcttc caacaggaaa acacatagga
     4261 tttaatgtct aataataaac ctttgaaaaa tagtactaaa ttacttgtta acttggacaa
     4321 gttcatattt ttagtcaatg ctgccgatag cctagaagaa attgaaatta ttagagattt
     4381 atgctgtgac tatttttcaa attgtaagag gtcaagctat tacattgata tttttgacga
     4441 tgcttattgg ataaaatatt acgaaatagc ttaatccttc cagattcgca taagagattt
     4501 tatgttaaat gccgaactga attaaaaata gaaatgtagt tgagctgatg cttgttcatt
     4561 tctttatttt tagtaattga ggtgaacata ctgtcagtac atggtagtta taagtaacgt
     4621 gtaactttgt ttgcacaaag atttgagtta tgccaagtga tggattgatt tgtacttaaa
     4681 attttaagct tatccacttg ctttgcaagc cactacactt tatttacaat cctatgttgt
     4741 ggaagtaagg gatcttgtac tcgcccagta tgttcaactt gattatatct aaaggactat
     4801 taggcttcaa tcaaatcaat tttccaatta gtcgactgaa ttaacacgtt taaatcacgt
     4861 aatttaagac taatatcatc agcttgtttt tgtaatgatg aaactggaat aactttatgc
     4921 cactttattt cccgcgtact ataacgatca gattcacgag atgtattact tattgctgca
     4981 gttagggttt tgtgctgttc tacaaattca tcacgcaaag tcaataaagc aagtagagaa
     5041 cggccatcat ttaattttgt taaagcatta gtttcatgaa ttgcataact taatttttga
     5101 aattcttgaa ttaaagaaaa tacttgtttt agtagttcat ttggatcttc agatggttga
     5161 tcaccatctt gtaccaatac atttgcattg atacgttgtt ttagtgaaat aatttttttt
     5221 tgttgatcac ttcgaagtaa gagagcttct gctaatttca tttttcacct attaaccaat
     5281 tagaattaag ttttaaacag cttatatcct cattctttaa agatcaaaaa aattgctttt
     5341 tatctagcta aaaggtgtct acaaaatcgg tggctattca gtgctgaaaa aggctgccga
     5401 cacataaagt gacccagatt gaagagttac tgccacactg ctggaaacct aaatcgaatt
     5461 aaaaaatagg tatgggattc agcggacgct taccttaatt attaatctaa tcattttata
     5521 agaaaaatct tctaagaaat tttcttagaa gattacagta tcttatctag aaattaagat
     5581 ttaagcctaa actaaaattt cttccaattt ggggaatata tggcaaaaat gtttcatggg
     5641 catagacttt ctgatcaagt aaattattcg ctttaaagaa taaatcatat tctgtgtgag
     5701 atagcttatt tttatatgct aaagtcatat taagcatatt ataacctgat gtaacctgct
     5761 caaatttaga aatattatct tgtttaaaga ctcgataata ttctatttca cctttaagcg
     5821 attcatcaaa atcagctttt agtcgtgtac ccaaacgagc aggaggtaat ctaggagtgt
     5881 aacgatcctt ttggggagct aaagtcggtt ctctattcca gatatcgtat gcaattacag
     5941 catcaggtaa atttgttaaa cggcctttca cataatctcc aaaaagagag ccgtgataaa
     6001 cagaattaaa ttgataccct atgttgccct ctaatccata aaaattagca gcagactggc
     6061 tataatgatt aatacgtagt gtatgctgat ctctaacttt tgtagttcct aaaacttcat
     6121 tgagtgtctg taaatatata tagttatcaa aatcataata gtaagtactg atttgataat
     6181 caagtaaatc atccttatag gccagactga tttctaaatt attggatttc tcttttctta
     6241 gaaagcggtt acctatttca aaagaattag tagcgagatg cataccatga gtatacattt
     6301 cttgtgcatt aggtaatctt tcttgatgtg tacctgttaa tgataagata taatttggag
     6361 caaatctcca gtggactgta cctgcataag aaaaagctgt ttctttgtta ggttgaacag
     6421 cttctaaaat agattttaaa ttttgagccc tgattttatt atttttttct acataaggac
     6481 tgttatattt attaggccaa ggtttcattg aatctttaat tttttctata tcataatcca
     6541 tactaacttt ctgcttttct atacgagcac ctagttcgaa agtaaagtcg ttccagttat
     6601 atttttctag gccaaataca ctaaagtttt tagtgacatt tcgatttaat agctgctgat
     6661 tatctagata ggtggtatgg ccttcttgag aatggactgg tgataaagca ctattatcct
     6721 gttccaaata ttgtagcccc aagataccgg ttaattcccc taatggctga tgtgataact
     6781 ctatgcggcc tacttttcct gtatttttaa aaaagttagt aatcttatct ccctcgagtt
     6841 cattgtgaaa ataatctata taactcaatg aggtacgaat tttgtcaata ccggtaaatg
     6901 gttgagtgaa ttctccacgc acatcataac gtcgagtatt taaatcaata taaggagcat
     6961 tatgagagaa attagtttca tgaatatgat tatgctgatg acaatcagga cgtggattta
     7021 tgtaattaat atcttgctca tccatcaatt gaggataata taaaagataa ttttttaatc
     7081 cactaattcg tgaatcgata ccgattgcag aggcaccaca accttcatat aaatggctat
     7141 gggctggtaa accatattca tcatgacgat gtgtataaga aactcctaaa taagtattat
     7201 cgtctatcca ggataatcca agcgtaccta ctctcgactc tgcccagctt tctggtaaat
     7261 attttaaatt tttataaata ctgttttggt tttcaagatc ttctaaagaa ttataagagc
     7321 tagactggta atgaggagtt ttatagttac cagcattacg atataaccct tctgcccgta
     7381 aagctacatg tgggcttaag ccaactgtta cacctgctgt ggtcaacttc tcattactgc
     7441 cagtattaaa acgtaatgta gtattaccct ctaatccgtc ttgaggcatt tgggttggaa
     7501 ttttatagtc aatgacattg actacccctg ccgcactatt tgaactatac aaaagggtac
     7561 ttgcaccacg tataatttca atagacttag ctaaacttgg ttcaactgtg acagcgtgat
     7621 ctggggacat attagacata tccaacacgt cagcattatt ctgtaaaact ttaatacgtt
     7681 ttccttcctg cccacgaata ataggagtag atgctccacc tccatattga ttagaatgaa
     7741 ttcctaattc cgtacccaag gcatccccta gagttgtcga acgctgttta agtttactag
     7801 tatcaataaa ctgattacta acatttagac ttttgcctaa cagctcagac cctaactcat
     7861 cagattgtgc agttaatact attgtattta aggtagtaac aggcccttca gctgccaata
     7921 aaattttcga ggcccctaaa gtaattaaag ctaaaaatag tttactttta tttagcataa
     7981 aacaaccaaa tttaatgtta tattataata taacacttaa aaatttataa aatctactat
     8041 ttaaattttt gaatggtaat cccccctaaa aatgaagtca aaaatgggag cctaagctcc
     8101 cattcttatt gatgattttt taattctttt agcatagcat ctcgtaaaat ttcattcata
     8161 cgtgtttgat aacctttgcc ttgtgctttg aaccaagcaa gtacatcagc atctaaccga
     8221 attgaagttt gttgctttac tgggcggtaa aattgatttt ggcgtacagc attgctccag
     8281 tctgtaattt caggaatatc tgatagatct agctgatcat caggaaccgt gcctttagca
     8341 agcaagcgtt gaatttcagc atcttgcttc tcaccaaatt tctcattcag ctctttgtgt
     8401 gagtatctaa ccatgctcat atttgtttcg ctccgcttta gtgacttgcc ttgcactaat
     8461 gattcggatg atttcacagt catcttcatc aaaaatggtg tgagccacca ataacattag
     8521 tacgccttta actcgtccaa tggtttgcca acgttcttca ccattggtat gtctgtcttg
     8581 gattgagatc cgtaatggat cttcaaaaac aaggcttgca gtttcgaaag agatatcatg
     8641 ctttttctga ttctttcgat tcttagcctc atcccattca aaatactgtt ccataaaaaa
     8701 cattcaaatt tgtatataca ataatgtatc acaaaattgt atcactcaac cagattcgtg
     8761 taatttttag tcaaaattat tggcaagaag tatcaaaact gctcataaaa aagccgactt
     8821 gtttcaaagt cggctttttg ggcattttgg gatttttcta tttttattaa gtagttgata
     8881 tttatattaa tatacaagtg tacttcgtat aatcgccatt atgttaaatt aaaagagttt
     8941 taactttttt aataaaaata ttatgctcta agccccaaaa tttcccctaa catgaatttg
     9001 taatgaaaaa atttatactt cctatattca gcatttctat tctagtttct ctcagtgcat
     9061 gttcatctat taaaactaaa tctgaagata attttcatat ttcttctcag caacatgaaa
     9121 aagctattaa aagctatttt gatgaagctc aaacacaggg tgtaattatt attaaagagg
     9181 gtaaaaatct tagcacctat ggtaatgctc ttgcacgagc aaataaagaa tatgtccctg
     9241 catcaacatt taagatgcta aatgctttaa tcgggctaga aaatcataaa gcaacaacaa
     9301 atgagatttt caaatgggat ggtaaaaaaa gaacttatcc tatgtgggag aaagatatga
     9361 ctttaggtga ggcaatggca ttgtcagcag ttccagtata tcaagagctt gcaagacgga
     9421 ctggcctaga gctaatgcag aaagaagtaa agcgggttaa ttttggaaat acaaatattg
     9481 gaacacaggt cgataatttt tggttagttg gcccccttaa aattacacca gtacaagaag
     9541 ttaattttgc cgatgacctt gcacataacc gattaccttt taaattagaa actcaagaag
     9601 aagttaaaaa aatgcttcta attaaagaag taaatggtag taagatttat gcaaaaagtg
     9661 gatggggaat ggatgttact ccacaggtag gttggttgac tggttgggtg gagcaagcta
     9721 atggaaaaaa aatccccttt tcgctcaact tagaaatgaa agaaggaatg tctggttcta
     9781 ttcgtaatga aattacttat aagtcgctag aaaatcttgg aatcatttaa taatttctaa
     9841 aattaacata atacacctta tgcgaagccc agattaatcc tgggcttttt tttcaatttg
     9901 cttattagct gtaaacattc caggattagt ggttcggatg ttttcctgac agctactaca
     9961 aagcattaac cctgcaaaaa gttgtacatt ttcctttgat tcacagaatc cacatgattt
    10021 aggaaaagga acgatatttg ctaagttgac taattctttt gacataatgt ttatcctcaa
    10081 cttgtacata ttaatttcaa tatttttcag tatgttatac ctaatttcac gttaaacttt
    10141 gtacattctt gtataatcca tgtgtacaaa ctctttttta taaatttcat atatttaagc
    10201 ctaaaaaacc attaatttat gtacaacact atgtacatac taaaaactat ataaatcaaa
    10261 aaattagtat taaaaagatc aattattttg tacacaattc agaacctata aaaacaacca
    10321 aaaaccttat gaaataaagc tttgaaggtc atcatcttta gatgcgtggt gtgtgacaaa
    10381 aaaatcccgg catgtgccgg atcttggatt ggaaaattgg ctaaagtgac gtagggccgg
    10441 tgcttggttt tgcatggaaa aaagtattta tttccttgtt tagaaaaacg taaaaaatca
    10501 attttttgtc cattcatcca ggttaaaaat ttcaacccaa aactttaatt atgaaaagct
    10561 tcatagaacg cgttcaaatg cgatttaaga gcctttacct agaaaacata gatctcatag
    10621 tgaaaaacac aaaacagctc aaaaaacgca aatgagagcg aaataaagag gtattttgac
    10681 tttagataga tgcatagagc gagtgtctac gagcgaacta tcaaaatttg cgcctagacc
    10741 ctctgaaaaa catttttttg tcctctttag cctaagaaag cgtaagtttc gtgcagaaat
    10801 ttgctcctgg atcgagcgta gcgagtaaaa aagttttatg agcgaagcga attccaagtt
    10861 tcttttgctt ttttttaaa
//
