LOCUS       NC_019253               6037 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Enterococcus faecium plasmid pHY, complete sequence.
ACCESSION   NC_019253
VERSION     NC_019253.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Yamashita,H., Tomita,H., Inoue,T. and Ike,Y.
  TITLE     Genetic organization and mode of action of a novel bacteriocin,
            bacteriocin 51: determinant of VanA-type vancomycin-resistant
            Enterococcus faecium
  JOURNAL   Antimicrob Agents Chemother 55 (9), 4352-4360 (2011)
   PUBMED   21709077
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Yamashita,H. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2010) Contact:Hitoshi Yamashita Gunma University
            Graduate School of Medicine, Department of Bacteriology; 3-39-22,
            Showa-Machi, Maebashi, Gunma 371-8511, Japan
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009940945.2-RS_2024_05_27
            Annotation Date              :: 05/27/2024 02:32:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 7
            CDSs (total)                 :: 7
            Genes (coding)               :: 7
            CDSs (with protein)          :: 7
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AB570326.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VRE38"
                     /db_xref="taxon:1352"
                     /plasmid="pHY"
                     /note="VanA type vancomycin resistant"
     gene            199..633
                     /gene="bacA"
                     /locus_tag="HXC20_RS00005"
                     /old_locus_tag="D687_p7"
     CDS             199..633
                     /gene="bacA"
                     /locus_tag="HXC20_RS00005"
                     /old_locus_tag="D687_p7"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="WP_002351079.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="bacA"
     gene            1094..1474
                     /locus_tag="HXC20_RS00010"
                     /old_locus_tag="D687_p1"
     CDS             1094..1474
                     /locus_tag="HXC20_RS00010"
                     /old_locus_tag="D687_p1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002292464.1"
                     /translation="MSEQNQNLASDPSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1456..2370
                     /locus_tag="HXC20_RS00015"
                     /old_locus_tag="D687_p5"
     CDS             1456..2370
                     /locus_tag="HXC20_RS00015"
                     /old_locus_tag="D687_p5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002303564.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEYQIAIYTHTDKEHIHNHI
                     VINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMAEIKIKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYDQFCDSLKTFGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2330..2608
                     /locus_tag="HXC20_RS00040"
     CDS             2330..2608
                     /locus_tag="HXC20_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033627308.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLAPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKAFIQRSMDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            2610..3299
                     /locus_tag="HXC20_RS00020"
                     /old_locus_tag="D687_p8"
     CDS             2610..3299
                     /locus_tag="HXC20_RS00020"
                     /old_locus_tag="D687_p8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303565.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFLLG
                     CIFSLIIVSSGLLGVLEGNKRVSRSILLGNVTQKYESARNKKKKEQALAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEPQKEYQEKYEQVKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNIQSPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            4143..5087
                     /locus_tag="HXC20_RS00030"
                     /old_locus_tag="D687_p3"
     CDS             4143..5087
                     /locus_tag="HXC20_RS00030"
                     /old_locus_tag="D687_p3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            5108..5644
                     /locus_tag="HXC20_RS00035"
                     /old_locus_tag="D687_p2"
     CDS             5108..5644
                     /locus_tag="HXC20_RS00035"
                     /old_locus_tag="D687_p2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002303560.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDRLHEYEKDNDKNIYISSKNVVVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLVYTEETPEDESTTILSEETESI
                     EKHTKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     Typing          4144..5087
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1457..2367
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.684"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 accaattaaa aatcacaaag tgagcccctg tatcggttga tacaagggct acttggtata
       61 ctacacatta tgtctactaa cctttaaaat aacacgtttt ataaaataaa ccctcttgac
      121 aatatttaat tttctgatta ttctaaatat atacagaaag cgctttccta ggattttata
      181 tctaaggagg aagtaacaat gaaaaataaa attattaaaa ttattacttg tctatttttg
      241 ttcggtatga ttgttggagc aagtactccg ttaaatctgt ttccttttag tgctagaggt
      301 ataaaagtgg aagctgcaag ttcacgatat aatcataatc atcgtggatt tacttgctat
      361 aaaatgaact attatgttac taataaaatg tgtagagatt tgaaaaaaaa ttataataag
      421 ctaaagactc ctgctcaaat tgcatctttt attcctattg gtggagttgg aacatgggtt
      481 attacaaatg cttttagtag tgctgttgat gcaatgaatg tctttgtaag agctgcaaat
      541 caaggaaaag gtgtacaatt aacatataat gctcacttta gcaatacaac atcatatcaa
      601 tacaatgatt acgctcgtta tgtaataaaa taatcagaaa gtgaaactat ctcatgaata
      661 aaaagatttt agctgtaatt atttcaataa tagttttaat cactatgttc ttcatattta
      721 ggctaatttt caatataagc ttacaaaaaa gtattcttta tctaattcct atagcattta
      781 tcttagcaat ttttagaagt atttacggaa ataaaaactc taaatagcta aaaataaatc
      841 atactaaatt ttacaaaaag tggctagcat tttctagcca ctttttgtat tgtggagcgt
      901 gggttttgag ggggattcaa gggggagctt gcgccccttt gacaagcggt tatttgcacc
      961 aaatacggag gtatttggct agcaaatata gagcttgcca aaccctttta gtgagcttta
     1021 ctcattgtac ctttttaggt ataatggtat tgcgaacgat acgtccgtag gacgtactga
     1081 aagggtggga cttatgagcg aacaaaatca aaatttggct agcgatcctt ctaaaaaata
     1141 tacctatcga tccgagccaa aacaaatcag ttttcgagtg agcgaatccg aatttgcaaa
     1201 gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg gctttcgtga aagccaaggc
     1261 acaaaacgcg cgtctggtga caccgaaagt ggcgccagac attgcccagg caatcgcgcg
     1321 cgatttagcc aaagctggag ggaatatcaa tcaaattgct aagtggtgca atacacacca
     1381 acatgacgtt gctcctggtg acgctcaacg tttatccgaa aacctaaaaa tcatgcaaaa
     1441 ggagctacaa aagatatggc aacagttaaa gtaagtcgaa ccacttcatg tagccgagca
     1501 atcaattacg cagaaccacg agcaaccgtc aaaacaggaa taaactgtga cattgattat
     1561 gccaaaagtg aaatgaaaca aatacgcatg ctttatggaa aagacgatcg tgtacaagca
     1621 catttattga ttcagtcctt tcgaccagga gaaattaccg ctgaaaaagc caaccaacta
     1681 gggaaagaat acgctgaaaa aatcgcgcca gaataccaaa tcgcgattta cacgcacaca
     1741 gataaagagc atatccacaa tcacattgtc attaattcgg tcaatttaga aacaggaaaa
     1801 aagtttcaag cacatggtca agcattccta gataagtgtt acgatatcaa cgacgaaatt
     1861 tgtttaactc atggtttaag tatcactgaa cgaggaaaaa aagaagaaaa acgaaccatg
     1921 gctgaaatca aaataaaaga aaaaaatgaa cctgtttgga aagatgaaat tcggtttgcg
     1981 attgatcaaa ccatgaaaaa caagaaaacc agaacctatg accaattttg tgattccctc
     2041 aaaacattcg gcattcattg cttcaatcgc gggaaaaatt tcacttacga attgatccac
     2101 aaaaagaaaa aagtacgatc caacagacta ggaaaggact acgaaaagga gacgatttta
     2161 catgagctgg acagacgaga acgaacctat aaccaacagc gaaccaacga gctacgaaga
     2221 gctcgagcta gcattcgcga aactgcagaa gaagtacgaa gagaacaacg aactcttacg
     2281 cctgaacaac cggaaattag accggctcga agaaaccatg aacaacacgt tgaaagagga
     2341 acagaccacc aacaagaatt atcgctctaa aacgttaaaa gacatagaaa acgaaataaa
     2401 gaaacaacgt gcttttcttg ctccctacac acaagaaagg atacaaacta cgattgattt
     2461 acaagttaaa aaactccatc aagagctatt agatcagcga aataaagaac aaaaaaaggc
     2521 gttcattcaa cgatcaatgg attttcttgc ccccttatgt tgcttagtcc tgacactgtt
     2581 cgcattttgg ttagtatggg gaggctaaaa tggcatttta tttaccctat ctactcattt
     2641 ttgttagtat ttcaggaagt atttggctca tctataaaat tttccaaaca agatattcat
     2701 taaaaggctc taaaattcga tttaagcgat ttttcttgct tggctgtata ttttccttga
     2761 taatcgtttc aagcggctta ttaggcgttt tagagggcaa taagcgcgtt tctagaagta
     2821 ttttattagg aaatgtcact caaaagtatg aatcagcacg taataaaaag aaaaaagagc
     2881 aagccttagc acaaaaaata gaaaaattta ccgcttgtta tgaggacatg aatgatattt
     2941 ttgtgaagca agaaaagcgt ttgacggata aaaatatgga aacactcact cgactttatc
     3001 ggaatttgcc agaagaaccg caaaaagaat accaagaaaa atatgaacaa gtaaaaaagg
     3061 acgtgcaata tgtaaaagat acaaagatag aagaagcctg tagtgatcta tttggagaca
     3121 caaacccttg gtttgccagt gaagaagaaa aaaaagaaaa acaacaatct gtcacatatg
     3181 aacgctacga aaacctgttt caacaagcaa ctaacataca aagtccaacg aaaaaagaaa
     3241 cggcattaaa ttatctagaa agtgtcaagg aatggttaga tcagcaacag caaaattgaa
     3301 tttgatcggc aatcatgtta aactaaacat gcaaatgatc aaacattaga tacacgaaag
     3361 ccccagagag gtcggattct ctggggcttt ttttcaggtt gatgtcgcct ttattgattt
     3421 tacttcttgt ttcggttatt caaccaataa ctgaataaag agacacaaac ttctataaga
     3481 agggggcaaa tgaacaattc aaacattaga tacacctcct tccagtcgca agactgtcgg
     3541 ataggcgaca agttattata tcttaaaaaa ttaaacttgt gtggtgtttt taagtatcgc
     3601 ttattttagg gttgttaggg cttgccctga ccgcctgtaa ggcgctttaa agctagataa
     3661 ggatttttat ccaagactgt cttaaaacgg cttagataag cattcacaac cgctcaaagg
     3721 cctctttacc aacatgtttt tagcaaagga gcctttaggc gactgcgcag tacaatgtga
     3781 gagcgtagcg atcacacctt tttggcgacc gtaggaagca aggttttgga ttggcagttt
     3841 tgaaaaatgc gcgaacctgt ttaaagaaaa aagaagtggt ttttaggcgg gaaaattagc
     3901 ccccctcttt ttatatctct ttttaaacct ctttttaaac ctctttaccg taagctgtgt
     3961 ccttactcta ccaatggata cagaatgtta tgaaggacat tttgtctgtt tatgaaggac
     4021 attttgtctg tttatgaagg acattttgtc tgtttatgaa ggacattttg tctgtttgaa
     4081 cgtcaaatat gaatgtcttg acatttaaaa aatgtcattc ataattggat tgaggtgatt
     4141 atatggctaa tgagattgta aaataccacc atgaattaaa tacaattcct ttaagaaaat
     4201 ttacaccggt tgaaatgaat ttgttttttt ccatcgtgtc tagaatgcgt gaaaaagggg
     4261 ataagacagt tcgttttacg tttgaccaac taaaagattt aagtaactac aaagcaactg
     4321 caaatgtacg atttgtagaa gatttaaaag caacttatga aaaaatctta tctttacgct
     4381 ttggtcgtag aagtgagtct gggctttctt acgatatgtt tgtaatgttt acagaattta
     4441 aaatcaacgg ggacgtagaa aatccttacg tagatatacg aatttacgaa aaagcactgc
     4501 ctttgttaaa taatttagac gagtgggtac gctattcttt acaacaattc aacgagctac
     4561 aaagtggcta tgcaaagact atgtttcgtc tgttaaagca atacagaacc aaaggtttcg
     4621 catatttctc caaagaagac tttcttgaat tattagatat tcctaaaagt tataagcaac
     4681 ctgatataga taaacgagtt ataaaaccta tacgtcaaga actaactgca atcttcaaag
     4741 gactaacaat caaaaagaaa tacggaaaag gacggggaaa acctgttatt ggctatcaat
     4801 ttacgttcaa acctgagatc aaaaaagcag acgactttaa caaacatgca ccaaccaaag
     4861 aagaccaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa gaacccgcta
     4921 caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc caacaaatca
     4981 aacaactaga aaaagagaaa aaagctcttg aaaaacaaac agagtccgca ctttcggaaa
     5041 aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa cctaggaggc
     5101 aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagatc tttttaagat
     5161 gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga tagctaaaaa
     5221 tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgcta gcaacttagg
     5281 taaccaaagg ttactagata ggttgcatga gtatgaaaaa gacaacgata aaaacattta
     5341 tatatcaagc aaaaacgtgg ttgtaaacga gttgctagat aagttgcgtg aagaaaatga
     5401 atttttacga gaacaacttt cccttaaaga gcaaaataat tctaatatgc aaaaattact
     5461 cgaccagcaa caacagctca ccctacaggc aaatcaacag atcgaaaaac tgcaagaaca
     5521 gttacaatta gtttatacgg aagaaactcc agaagatgaa tcaactacta ttctttctga
     5581 agaaacagaa tcgatagaaa aacacacaaa gaagaagcaa aaatggtggc aactttggaa
     5641 ataactggag ggaatattca tgacaagaaa aaatcttcta ttcggttcta ttttactagg
     5701 aattctaggc ttcagtccga tgttattgtc agtgatcaat aaagggaata aacatggtga
     5761 attgaaattg aatccaaatt attacaacag tcctaacgaa gaattgtttg taaaagaaac
     5821 ggataaataa taaaaaagac tgctctttcg agcagccagt taaaaaaata atgagagttt
     5881 attcttgaaa aagcaacgcg cttataaagc gctaccaaaa ttttatcata ttttataaaa
     5941 aataacaatc cagtaaaaaa tgactggtaa aaattcacaa agaaacggaa agaagcaact
     6001 gcagaaaatc aagccttaaa aagagctttt gacctag
//
