LOCUS       NC_019250               4043 bp    DNA     circular CON 10-SEP-2023
DEFINITION  Shigella flexneri plasmid pSF301-3, complete sequence.
ACCESSION   NC_019250
VERSION     NC_019250.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226109
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Gao,X.P., Zhang,X.B., Yang,J., Liu,L.G., Zhao,L.N. and Jin,Q.
  TITLE     The complete sequence analysis and characterization of three
            plasmids from Shigella flexneri 2a 301 strain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Gao,X.P., Zhang,X.B., Yang,J., Liu,L.G., Zhao,L.N. and Jin,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-APR-2011) Institute of Pathogen Biology, State Key
            Laboratory for Molecular Virology and Genetics Engineering, Chinese
            Academy Medical Sciences & Peking Union Medical College, 6 Rong Jing
            East Street, BDA, Beijing 100176, China
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/10/2023 04:19:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 6
            CDSs (with protein)               :: 6
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JF813188.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /strain="2a 301"
                     /db_xref="taxon:623"
                     /plasmid="pSF301-3"
     gene            complement(315..506)
                     /locus_tag="HPE76_RS00005"
                     /old_locus_tag="D742_p3001"
     CDS             complement(315..506)
                     /locus_tag="HPE76_RS00005"
                     /old_locus_tag="D742_p3001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165994.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            571..894
                     /locus_tag="HPE76_RS00010"
     CDS             571..894
                     /locus_tag="HPE76_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956004.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1103..>1534
                     /locus_tag="HPE76_RS00035"
                     /pseudo=""
     CDS             1103..>1534
                     /locus_tag="HPE76_RS00035"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1572..2102
                     /locus_tag="HPE76_RS00020"
     CDS             1572..2102
                     /locus_tag="HPE76_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067502.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETNWTM
                     ENGKQNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            2109..2342
                     /locus_tag="HPE76_RS00025"
     CDS             2109..2342
                     /locus_tag="HPE76_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137443.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2888..3310)
                     /locus_tag="HPE76_RS00030"
     CDS             complement(2888..3310)
                     /locus_tag="HPE76_RS00030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_167709800.1"
                     /translation="MLPREQNCVAMALSACFRQQLNPVVNSLLKERIIHSPKELEHDNA
                     VIRVLQKLQIQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYK
                     NAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
                     /name="hypothetical protein"
     gene            complement(3597..3755)
                     /locus_tag="HPE76_RS00040"
     CDS             complement(3597..3755)
                     /locus_tag="HPE76_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005093880.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEIL
                     RVLEVVA"
                     /name="hypothetical protein"
     Typing          210..296
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          complement(2..199)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.99"
                     /coverage="100.0"
                     /evalue="7.09e-92"
     Typing          complement(3647..4043)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.98"
                     /coverage="92.0"
                     /evalue="0.0"
     Typing          885..2446
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.313"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttttgctca catgttcttt ccggcgttat cccctgattc tgtggataac agcatttcgt
       61 ttttgagtga gtagataccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagttga
      121 gcgaggaagc ggaaaagcgc ctggacgtgc attttctcct tacgcatctg tgcggcattt
      181 cacacccggt atggcgcact tttcatacaa tccgcactga tgccgcatgg ttaagccagt
      241 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgctaacacc
      301 cgctgacrmg cccgtcagag gttttcrccg tcatccccga agcgagcgag gcaactgcgg
      361 taaagctcat cggcgtggtc gtaaagcgat tcacagatgt ctgcctgttc atccgcgtcc
      421 agttcgttga gcttctccag cagcgttaat gtctggcttc tgataaagcg ggccatgtta
      481 agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg
      541 ggggtaatga taccgatgaa acgcgagagg atgctcacga tacgggttac tgatgatgaa
      601 catgcccggt tactggaacg ctgtgagggt aaacagctgg cggtatggat gcggcgggtc
      661 tgcctgggtg agccggttgc ccgttccggg aggctgccga cactggcacc gccgttactg
      721 cgtcagctgg ccgccatcgg gaataacctg aatcaggcag cgaggaaggt gaacagcggg
      781 cagtggtctt ccggtgaccg ggttcaggtg gtggccgcac tgatggccat cggggatgag
      841 ctgcgccggc tgcgtctggc cgtcagggaa caggggacgc gggatgatag ttaagtttca
      901 tgccagggga aaaggcggtg gcagtggtcc ggttgattac ctgctgggaa gggagcgtaa
      961 ccgggaaggg gcaacggtgc ttcagggtaa tccggaagaa gtccgggaac tcatcgatgc
     1021 cacgccattt gcgaagaaat acacgtccgg tgttctctcg ttcgcggaga aggagctgcc
     1081 gccgggaggg cgtgaaaaag tgatggcgag ctttgagcgt gtactgatgc ccggtctcga
     1141 aaaaaatcag tacagcattc tgtgggtgga gcaccaggac aaggggcggc ttgagctgaa
     1201 ttttgtcatt ccgaacatgg agctacagac cggaaaacgc ctccagccgt actacgaccg
     1261 cgcagacagg ccgagaattg atgcctggca gacgctggtg aatcaccatt acgggctgca
     1321 tgacccgaac gccccggaga accgccggac gctgactctc cctgataacc tgccggaaac
     1381 gaaacaggcg cttgctgaga gcattacacg gtggatagac gctctttatc atgtcgggga
     1441 gataaaaggc cgtcaggatg tgattcaggc gctcacagag gcggggcttg aagtggtcag
     1501 ggtgacgcga agcagtatca gcattgcaga cccgaacggc gggaagaata tcagactgaa
     1561 aggagcattc tatgagcaat cttttacaga cgggcgcgga gttcgagaaa aagctgaaag
     1621 agagagccga atctacagag aaaatgctga gcgacgagtt caggaagctc gaagaatctg
     1681 taagcaaggc tgtgacatca aacgagacga aaatcaaaga cgctatagcc ctgttcacag
     1741 cttcgacaga ggaatcactg aaaaaacatc gggaaggggt gaaagaggcg atgatgcagc
     1801 acaggaagga cgtgttaaag ctggcaggga atacgggcat gatgttactg gggatggtca
     1861 ttttcctgtt taccgtgagt ggcgggacgc tctggtatct tggcgggatg atacaggcga
     1921 acctggaaga aatcaggaag cagaacgaga gtctggagaa gctgaacgcg aagacatggg
     1981 gcgtgcgtta tcacgagggg agcaacggac gttttctggt gttgcccgag gggatgaaag
     2041 ccgaaacgaa ctggacgatg gaaaacggga aacagaatgc cgtaaggctg gtacaggagt
     2101 gacagagcat gacggagctg gaaagccatt tgctgaacgc attagagcag ctgcaacagg
     2161 actatatgca gcggctgaac gaatgggaga gcgccttcgt ggaattgcag aaaatgtttt
     2221 cgcttacgca acgggacaac gcgatgctga acgagcgggt catgcagttg agtcagcagg
     2281 tgctgcactt gagcgagcag acagaacgct tgagccagtt atacagcgag aactggagat
     2341 aagagaggaa cggttgatac aggagcgcga acatgcgtta ttcctggaac gggaacgcca
     2401 gccggaaata caggaacgga cactggatgg cccttcgctg ggatggtaat cagagacagg
     2461 ctaaaatgtt gtcagcgcgt ttccggtgca ttttttggga taatggttgg ttcgggggca
     2521 ccgggaacgc gcttcagtca gaccttrctg aaaacacagg aacgagttaa aacgcatacg
     2581 ggcgcgattc tggtgtgtct tttgtcgctg tgttttgcct ctattgccag atcgcgctca
     2641 ttcccctgaa cttctgtttt cccgctcagg tccccgtctt cttcgcttcc gaaagcatga
     2701 ataaaatccg tttcagctgt ctctggctgg caattcctgc gtgattcaca tggctgcata
     2761 gctatgcatg tggttaaaat ttaccgggtg cgatcgcggc agtttttcgg gtggtttgtt
     2821 gccattttta cctgtctgct gccgtgatcg cgccgaacgc gtttcagcgg tgcgtacaat
     2881 taagatatta cgttaaattg tgagggaatg gtccccatat actgacttta tcatagggat
     2941 agggttggta gtgggtcgat tgggtttcgg cattgtttcc gttaatgcca attgcatttt
     3001 tgtatttgat gcaacagaag gcgtgagatt ctcctgggcc tggaaaggct aagtgtttag
     3061 aattgatggc aaaatatctt ccgtccgatt tttgaagtag ttgttgtttg gctgtctccc
     3121 aaagggtgct gttacaaact tcttgaattt gtagtttttg caggactctt attactgcgt
     3181 tgtcatgttc caattcttta ggggagtgta ttattctctc ttttaataag ctgtttacca
     3241 cagggtttag ttgttgtcta aagcacgcag ataatgccat tgctacacaa ttttgttccc
     3301 gaggaagcat gtttggcggg gtatgccgtg tttgatgtga tatgtctatt ctcatagaag
     3361 gtctctggct tatagcatca acaaatacgg ctataaaaca aaattttcat agagtgattc
     3421 aagatcttct tgagatcctt ttttgcggcg cgtaatctgc tgcckgcaaa caaaaaaacc
     3481 accgctacca acggtggttt gtttgccgga atctagagct accaactctt tcttccgaag
     3541 gtaactggct tggtggagca cagataccaa atgctgtcct tctagtgtag ccgcagttag
     3601 gccaccactt caagaactct taatatctca atccaccttg tccagttacc aatggctgct
     3661 gccagtggcg ttaagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3721 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3781 tasaccgaac tgagatacca acagcgtgag ctatgagaaa gcgccrcgct tcccgaaggg
     3841 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3901 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3961 gagcgtcggt ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccatcggc
     4021 gcggccttct tcctgttcct ggt
//
