LOCUS       NC_019249               3178 bp    DNA     circular CON 10-SEP-2023
DEFINITION  Shigella flexneri plasmid pSF301-2, complete sequence.
ACCESSION   NC_019249
VERSION     NC_019249.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226057
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Gao,X.P., Zhang,X.B., Yang,J., Liu,L.G., Zhao,L.N. and Jin,Q.
  TITLE     The complete sequence analysis and characterization of three
            plasmids from Shigella flexneri 2a 301 strain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Gao,X.P., Zhang,X.B., Yang,J., Liu,L.G., Zhao,L.N. and Jin,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2011) Institute of Pathogen Biology, State Key
            Laboratory for Molecular Virology and Genetics Engineering, Chinese
            Academy Medical Sciences & Peking Union Medical College, 6 Rong Jing
            East Street, BDA, Beijing 100176, China
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 09/10/2023 04:18:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JF813187.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /strain="2a 301"
                     /db_xref="taxon:623"
                     /plasmid="pSF301-2"
     gene            join(2111..3178,1..42)
                     /gene="wzz(fepE)"
                     /locus_tag="HPE71_RS00005"
                     /old_locus_tag="D742_p1"
     CDS             join(2111..3178,1..42)
                     /gene="wzz(fepE)"
                     /locus_tag="HPE71_RS00005"
                     /old_locus_tag="D742_p1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="WP_015060455.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVIL
                     FFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFALF
                     IKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKNDKS
                     SLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEILALD
                     EIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIATKLNI
                     KKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGXSNAIIVI
                     LSSVIGGMLACGYILLRHAMEARKMSVIE"
                     /name="wzz(fepE)"
     gene            complement(287..421)
                     /locus_tag="HPE71_RS00015"
     CDS             complement(287..421)
                     /locus_tag="HPE71_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071606675.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY"
                     /name="replication initiation protein"
     gene            complement(1065..1229)
                     /locus_tag="HPE71_RS00010"
                     /old_locus_tag="D742_p2"
     CDS             complement(1065..1229)
                     /locus_tag="HPE71_RS00010"
                     /old_locus_tag="D742_p2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_015060456.1"
                     /translation="MARCIKSQSLTLLEKLDALDADEQAXMCKRLHELAEELQNSIQIR
                     FEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          899..985
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          1300..1398
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          1523..1780
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.385"
                     /coverage="89.0"
                     /evalue="3.4e-112"
     Typing          complement(350..874)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.591"
                     /coverage="98.0"
                     /evalue="4.9000000000000006e-173"
ORIGIN
        1 cgtcatgcca tggaggccag aaagatgagt gtgattgagt aaaagttgtg ctgatgcatc
       61 attttataag caacttttat aaaaagttca aaggatcttc ttgagatcat tcccttacag
      121 ccgtagtctt ctactctaga aaacgaaaaa accacctggg gaggtggttt ttcgaaggtt
      181 cagtaagttg gggaacttct gaaccgtggt aacagtcttg tgcgagacat gtcaccaaat
      241 ctgtccttct agtgtagcct cagtaaggct gccacttcaa gaactctcaa tacatctctc
      301 gcacatcctg tttaccagtg gccgctgcca atggcgttaa gtcgtgtctt accgggttgg
      361 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
      421 cacagcccag cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagac
      481 gaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc
      541 acgagggagc caccaggggg aaacgcctgg tatctttaag tcctgtcggg tttcgccacc
      601 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
      661 ccaccggcgc ggcctttttt cggggcctct tttttgctca catgtttttt ctggtgttat
      721 cccctgattc tgtggataac cgtatatccg tctttgcgtg agcagacacc gctcgccgca
      781 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaragcgcc ggaagctgca
      841 tttctcctta cgtatctgtg cagcatttca cacctcatat gtcgctgttc tctcaccatc
      901 tgctctgccg cctcataatt aagccagtat acactccgtt atcgctacgt gactgggtca
      961 tggctgcgcc ccgacacccg ctaaaacccc ctgacgcgcc ctgacgggct tgtctgctga
     1021 cgaaaacgcc cacgggaaag ccgtgaccgc ctccgtcagc ttcgttatgt tcctgtttca
     1081 ctttcggcct caaagcgaat ctggatgctg ttctggagtt cttctgcgag ttcgtgcagt
     1141 cgtttgcaca tgkccgcctg ttcgtcggca tccagagcat ccagtttttc aagcagtgtc
     1201 aggctctggc ttttgataca tcgcgccata ttcagcgcag tttgttgtta tttgttcacc
     1261 ttccgttgtt ctccgtcagt tccgggtgtc gggggggggg accctgagca ggtggtaatt
     1321 gtaagacgtt gcgggcgacg gtattacaat tgcacatcct gtcctgtttc tttaggacgt
     1381 atcatggcac agcatatcaa ctgataaata atccctgtgc agtgcagccg ctgttgcata
     1441 aaatttcgct ctgcgtcgag tgtttcttaa tcaataattc cgctgatact ccggaaaaac
     1501 gcgagtctgt tctcatgcct gaaatgccca caccccacgc aaaaacaagt ttttgctgat
     1561 ttttctttat aaatagagag ttatgacaaa ttagttcttc ttgctctctt tgtgatattt
     1621 aaaaaagcgg tgtcggcgcg gtgttgtagc tgcgccaaca ccgcttttta ggggtggtac
     1681 tgactatttt cataaaaaaa acattatctt atattagggg tgctgctagc ggcgcggtgt
     1741 gtttttttac aggacgcccc tgggggcgct gctaggggtg tctgttcaga tatgtagcaa
     1801 tgagtcaggg attcacgaac tgaaaatgtc cgaaaatgtg tctcatgggc actgtatgcg
     1861 cccaaaccga tcgtcattca ctttcatgca tagctatgca gtgagctgaa agcgatcctg
     1921 acgcattttt ccggtttacc ccggggaaaa catctctttt tgcggtgttt gcggcagaat
     1981 cgcgttcagc gcgttttagc ggtgcgcgca ataaaatgtt atgttaaatg tctgctgaat
     2041 ggtttttagg ttttttccta acatatgata gaataaaaaa accatatgtt aatatattgt
     2101 gtttttttca atgaaaatct caaaggtctc tgacgaacat ttttctaatt acaacatgtt
     2161 gccgaatggt aatgaagaaa ttgacctttt tggcttgatt agcgttttat gggcagcaaa
     2221 aataaaaatt gttggggtga ttctattttt tgcttgtatt ggctttcttg tttcttttgt
     2281 tttgccacaa aaatggacta gttctgctgt ggttactcct gctgagtctg ttcaatggag
     2341 tgaattaaat aaggagcttt ccaagcttca tgttctggga gtggattttg atatcaatcg
     2401 tgatagcgca tttgctctgt ttattaagaa atttcagtcc gttaattcgt tgaatgagta
     2461 tatgagatca tctccttatg ttatggagat tattaaaaag aataacatta gcgctctcga
     2521 actgcatcgt gcaattgttg agttgagtaa aaatatgaag tcgattgatg ataatgccag
     2581 taagaaaaac gacaagtcat cattgtatgt atcatggact ctgagtttta ctgctccaac
     2641 aagtaaagaa gctcacgatg tgttgtctgg gtatattaat tatgtttctt cccttgttgt
     2701 aagggatttg atggaagata taagaaataa actagaagtt aaaactaatg ttgaaaaaga
     2761 aattcttgca ctggatgaga taaaaattag aaaccagctg aatgcagata ttcgacgcct
     2821 caattattca ctggaggttg ctaatgcggc tggaataaaa aaacctgtat acagcaatgg
     2881 tcagattatg aaggatgacc cagattttcc tgtggctctc ggttctgatg gtatagcaac
     2941 taaattgaac atcaaaaaat caatcaagga tgtttcggaa ttgagtgggg agttgcgaaa
     3001 tcgtcaatat gttgtgaatc aattggttgt ggcgaaagtg ggggatgttg attttatgcc
     3061 atttcagtat caattgagcc caacgttacc ggtcagaaag gatgggmcaa gtaatgccat
     3121 aattgtaatt ctttcttctg tcattggagg gatgcttgca tgtggctata tactttta
//
