LOCUS       NC_019199               8964 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Acinetobacter baumannii plasmid pMMCU3, complete sequence.
ACCESSION   NC_019199
VERSION     NC_019199.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225470
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Merino,M., Acosta,J., Poza,M., Sanz,F., Beceiro,A., Chaves,F. and
            Bou,G.
  TITLE     OXA-24 carbapenemase gene flanked by XerC/XerD-like recombination
            sites in different plasmids from different Acinetobacter species
            isolated during a nosocomial outbreak
  JOURNAL   Antimicrob Agents Chemother 54 (6), 2724-2727 (2010)
   PUBMED   20385865
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   Merino,M., Poza,M. and Bou,G.
  TITLE     Long persistence of a multidrug-resistant Acinetobacter baumannii
            clone harboring a plasmid expressing OXA-24 carbapenemase causing
            bacteremia in a tertiary hospital in Madrid
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8964)
  AUTHORS   Merino,M., Poza,M. and Bou,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2009) Microbiology, CH Universitario A
            Coruna-INIBIC, Xubias s/n, A Coruna, A Coruna 15006, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 11/28/2023 04:28:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 11
            CDSs (total)                 :: 11
            Genes (coding)               :: 11
            CDSs (with protein)          :: 11
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            GQ904227.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:470"
                     /plasmid="pMMCU3"
     gene            complement(223..684)
                     /locus_tag="HPE73_RS00005"
                     /old_locus_tag="D721_p01"
     CDS             complement(223..684)
                     /locus_tag="HPE73_RS00005"
                     /old_locus_tag="D721_p01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(989..3400)
                     /gene="znuD2"
                     /locus_tag="HPE73_RS00010"
                     /old_locus_tag="D721_p02"
     CDS             complement(989..3400)
                     /gene="znuD2"
                     /locus_tag="HPE73_RS00010"
                     /old_locus_tag="D721_p02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_012754356.1"
                     /translation="MLNKSKLFLALITLGASKIVLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            complement(3528..3842)
                     /locus_tag="HPE73_RS00015"
                     /old_locus_tag="D721_p03"
     CDS             complement(3528..3842)
                     /locus_tag="HPE73_RS00015"
                     /old_locus_tag="D721_p03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(3829..4116)
                     /locus_tag="HPE73_RS00020"
                     /old_locus_tag="D721_p04"
     CDS             complement(3829..4116)
                     /locus_tag="HPE73_RS00020"
                     /old_locus_tag="D721_p04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            4425..5252
                     /locus_tag="HPE73_RS00025"
                     /old_locus_tag="D721_p05"
     CDS             4425..5252
                     /locus_tag="HPE73_RS00025"
                     /old_locus_tag="D721_p05"
                     /EC_number="3.5.2.6"
                     /function="hydrolysis of beta-lactams"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-24"
                     /protein_id="WP_012754353.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MGVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-24"
     gene            complement(5435..5578)
                     /locus_tag="HPE73_RS00030"
                     /old_locus_tag="D721_p06"
     CDS             complement(5435..5578)
                     /locus_tag="HPE73_RS00030"
                     /old_locus_tag="D721_p06"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(5590..6165)
                     /locus_tag="HPE73_RS00035"
                     /old_locus_tag="D721_p07"
     CDS             complement(5590..6165)
                     /locus_tag="HPE73_RS00035"
                     /old_locus_tag="D721_p07"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_012754352.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTPQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATASPQQDATACDTDMQQHATPQQDNDNKKNHELNIAENVAMPQQENTAYHTQTLQH
                     ATLQNVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(6158..7108)
                     /gene="repM"
                     /locus_tag="HPE73_RS00040"
                     /old_locus_tag="D721_p08"
     CDS             complement(6158..7108)
                     /gene="repM"
                     /locus_tag="HPE73_RS00040"
                     /old_locus_tag="D721_p08"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_015060152.1"
                     /translation="MRELVVKDNALINASYNLDLVEQRLILLAIVEARESGKGRNANDP
                     LEVHAEGYINQFGVHRNTAYQALKDACNDLFARQFSYQKINERGNIENYRSRWVSEIGY
                     VDNEAVVKLIFAPAIVPLITRLEEHFTKYELQQVSNLSSAYAVRLYELLIAWRSTGSTP
                     IIEVSDFRQRIGVLDTEYKRMERFKTSVLELAIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDNPPIERDPNTLDLFTKMTDAQRHLFANKLSELPEMGRYSQGTESYPQFAI
                     RIAEMLQDPDRIKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            7794..8048
                     /locus_tag="HPE73_RS00045"
                     /old_locus_tag="D721_p09"
     CDS             7794..8048
                     /locus_tag="HPE73_RS00045"
                     /old_locus_tag="D721_p09"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014301.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMKPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            8319..8690
                     /locus_tag="HPE73_RS00050"
                     /old_locus_tag="D721_p10"
     CDS             8319..8690
                     /locus_tag="HPE73_RS00050"
                     /old_locus_tag="D721_p10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504219.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWTLMKNGASKDEV
                     RMMQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            8690..8863
                     /locus_tag="HPE73_RS00055"
                     /old_locus_tag="D721_p11"
     CDS             8690..8863
                     /locus_tag="HPE73_RS00055"
                     /old_locus_tag="D721_p11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282482.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQD"
                     /name="hypothetical protein"
     ARG             4426..5249
                     /name="blaOXA-24"
                     /gene="blaOXA-24"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-24"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(6159..7108)
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.054"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgaggtgaac atactgtcag tacatggtag ttataagtaa cgtgtaactt tgtttgcaca
       61 aagatttgag ttatgccaag tgatggattg atttgtactt aaaattttaa gcttatccac
      121 ttgctttgca agccactaca ctttatttac aatcctatgt tgtggaagta agggatcttg
      181 tactcgccca gtatgttcaa cttgattata tctaaaggac tattaggctt caatcaaatc
      241 aattttccaa ttagtcgact gaattaacac gtttaaatca cgtaatttaa gactaatatc
      301 atcagcttgt ttttgtaatg atgaaactgg aataacttta tgccacttta tttcccgcgt
      361 actataacga tcagattcac gagatgtatt acttattgct gcagttaggg ttttgtgctg
      421 ttctacaaat tcatcacgca aagtcaataa agcaagtaga gaacggccat catttaattt
      481 tgttaaagca ttagtttcat gaattgcata acttaatttt tgaaattctt gaattaaaga
      541 aaatacttgt tttagtagtt catttggatc ttcagatggt tgatcaccat cttgtaccaa
      601 tacatttgca ttgatacgtt gttttagtga aataattttt ttttgttgat cacttcgaag
      661 taagagagct tctgctaatt tcatttttca cctattaacc aattagaatt aagttttaaa
      721 cagcttatat cctcattctt taaagatcaa aaaaattgct ttttatctag ctaaaaggtg
      781 tctacaaaat cggtggctat tcagtgctga aaaggctgcc gacacataaa gtgacccaga
      841 ttgaagagtt actgccacac tgctggaaac ctaaatcgaa ttaaaaaata ggtatgggat
      901 tcagcggacg cttaccttaa ttattaatct aatcatttta taagaaaaat cttctaagaa
      961 attttcttag aagattacag tatcttatct agaaattaag atttaagcct aaactaaaat
     1021 ttcttccaat ttggggaata tatggcaaaa atgtttcatg ggcatagact ttctgatcaa
     1081 gtaaattatt cgctttaaag aataaatcat attctgtgtg agatagctta tttttatatg
     1141 ctaaagtcat attaagcata ttataacctg atgtaacctg ctcaaattta gaaatattat
     1201 cttgtttaaa gactcgataa tattctattt cacctttaag cgattcatca aaatcagctt
     1261 ttagtcgtgt acccaaacga gcaggaggta atctaggagt gtaacgatcc ttttggggag
     1321 ctaaagtcgg ttctctattc cagatatcgt atgcaattac agcatcaggt aaatttgtta
     1381 aacggccttt cacataatct ccaaaaagag agccgtgata aacagaatta aattgatacc
     1441 ctatgttgcc ctctaatcca taaaaattag cagcagactg gctataatga ttaatacgta
     1501 gtgtatgctg atctctaact tttgtagttc ctaaaacttc attgagtgtc tgtaaatata
     1561 tatagttatc aaaatcataa tagtaagtac tgatttgata atcaagtaaa tcatccttat
     1621 aggccagact gatttctaaa ttattggatt tctcttttct tagaaagcgg ttacctattt
     1681 caaaagaatt agtagcgaga tgcataccat gagtatacat ttcttgtgca ttaggtaatc
     1741 tttcttgatg tgtacctgtt aatgataaga tataatttgg agcaaatctc cagtggactg
     1801 tacctgcata agaaaaagct gtttctttgt taggttgaac agcttctaaa atagatttta
     1861 aattttgagc cctgatttta ttattttttt ctacataagg actgttatat ttattaggcc
     1921 aaggtttcat tgaatcttta attttttcta tatcataatc catactaact ttctgctttt
     1981 ctatacgagc acctagttcg aaagtaaagt cgttccagtt atatttttct aggccaaata
     2041 cactaaagtt tttagtgaca tttcgattta atagctgctg attatctaga taggtggtat
     2101 ggccttcttg agaatggact ggtgataaag cactattatc ctgttccaaa tattgtagcc
     2161 ccaagatacc ggttaattcc cctaatggct gatgtgataa ctctatgcgg cctacttttc
     2221 ctgtattttt aaaaaagtta gtaatcttat ctccctcgag ttcattgtga aaataatcta
     2281 tataactcaa tgaggtacga attttgtcaa taccggtaaa tggttgagtg aattctccac
     2341 gcacatcata acgtcgagta tttaaatcaa tataaggagc attatgagag aaattagttt
     2401 catgaatatg attatgctga tgacaatcag gacgtggatt tatgtaatta atatcttgct
     2461 catccatcaa ttgaggataa tataaaagat aattttttaa tccactaatt cgtgaatcga
     2521 taccgattgc agaggcacca caaccttcat ataaatggct atgggctggt aaaccatatt
     2581 catcatgacg atgtgtataa gaaactccta aataagtatt atcgtctatc caggataatc
     2641 caagcgtacc tactctcgac tctgcccagc tttctggtaa atattttaaa tttttataaa
     2701 tactgttttg gttttcaaga tcttctaaag aattataaga gctagactgg taatgaggag
     2761 ttttatagtt accagcatta cgatataacc cttctgcccg taaagctaca tgtgggctta
     2821 agccaactgt tacacctgct gtggtcaact tctcattact gccagtatta aaacgtaatg
     2881 tagtattacc ctctaatccg tcttgaggca tttgggttgg aattttatag tcaatgacat
     2941 tgactacccc tgccgcacta tttgaactat acaaaagggt acttgcacca cgtataattt
     3001 caatagactt agctaaactt ggttcaactg tgacagcgtg atctggggac atattagaca
     3061 tatccaacac gtcagcatta ttctgtaaaa ctttaatacg ttttccttcc tgcccacgaa
     3121 taataggagt agatgctcca cctccatatt gattagaatg aattcctaat tccgtaccca
     3181 aggcatcccc tagagttgtc gaacgctgtt taagtttact agtatcaata aactgattac
     3241 taacatttag acttttgcct aacagctcag accctaactc atcagattgt gcagttaata
     3301 ctattgtatt taaggtagta acaggccctt cagctgccaa tacaattttc gaggccccta
     3361 aagtaattaa agctaaaaat agtttacttt tatttagcat aaaacaacca aatttaatgt
     3421 tatattataa tataacactt aaaaatttat aaaatctact atttaaattt ttgaatggta
     3481 atccccccta aaaatgaagt caaaaatggg agcctaagct cccattctta ttgatgattt
     3541 tttaattctt ttagcatagc atctcgtaaa atttcattca tacgtgtttg ataacctttg
     3601 ccttgtgctt tgaaccaagc aagtacatca gcatctaacc gaattgaagt ttgttgcttt
     3661 actgggcggt aaaattgatt ttggcgtaca gcattgctcc agtctgtaat ttcaggaata
     3721 tctgatagat ctagctgatc atcaggaacc gtgcctttag caagcaagcg ttgaatttca
     3781 gcatcttgct tctcaccaaa tttctcattc agctctttgt gtgagtatct aaccatgctc
     3841 atatttgttt cgctccgctt tagtgacttg ccttgcacta atgattcgga tgatttcaca
     3901 gtcatcttca tcaaaaatgg tgtgagccac caataacatt agtacgcctt taactcgtcc
     3961 aatggtttgc caacgttctt caccattggt atgtctgtct tggattgaga tccgtaatgg
     4021 atcttcaaaa acaaggcttg cagtttcgaa agagatatca tgctttttct gattctttcg
     4081 attcttagcc tcatcccatt caaaatactg ttccataaaa aacattcaaa tttgtatata
     4141 caataatgta tcacatattt gtatcactca agctgaaaag cgtaattttt agtcaaattt
     4201 attggcaaga agtatcaaaa ctgctcataa aaaagccgac ttgtttcaaa gtcggctttt
     4261 tgggcatttt tggatttttc tatttttatt aagtagttga tatttaaatt aatatacaaa
     4321 tgtacttcgt ataatcgcca ttatgttaaa ttaaaagagt tttaactttt ttaataaaaa
     4381 tattatactc taagccccaa aatttcccct aacatgaatt tgtaatgaaa aaatttatac
     4441 ttcctatatt cagcatttct attctagttt ctctcagtgc atgttcatct attaaaacta
     4501 aatctgaaga taattttcat atttcttctc agcaacatga aaaagctatt aaaagctatt
     4561 ttgatgaagc tcaaacacag ggtgtaatta ttattaaaga gggtaaaaat cttagcacct
     4621 atggtaatgc tcttgcacga gcaaataaag aatatgtccc tgcatcaaca tttaagatgc
     4681 taaatgcttt aatcgggcta gaaaatcata aagcaacaac aaatgagatt ttcaaatggg
     4741 atggtaaaaa aagaacttat cctatgtggg agaaagatat gactttaggt gaggcaatgg
     4801 cattgtcagc agttccagta tatcaagagc ttgcaagacg gactggccta gagctaatgc
     4861 agaaagaagt aaagcgggtt aattttggaa atacaaatat tggaacacag gtcgataatt
     4921 tttggttagt tggccccctt aaaattacac cagtacaaga agttaatttt gccgatgacc
     4981 ttgcacataa ccgattacct tttaaattag aaactcaaga agaagttaaa aaaatgcttc
     5041 taattaaaga agtaaatggt agtaagattt atgcaaaaag tggatgggga atgggtgtta
     5101 ctccacaggt aggttggttg actggttggg tggagcaagc taatggaaaa aaaatcccct
     5161 tttcgctcaa cttagaaatg aaagaaggaa tgtctggttc tattcgtaat gaaattactt
     5221 ataagtcgct agaaaatctt ggaatcattt aataatttct aaaattaaca taatacacgt
     5281 tatacgaaat gctgtataat tatccttata attcaacgct gtagtttcta gtttatagcc
     5341 cgagcatccc tgttcgggct ttttttatga cttttcacgt tccacgctat tatttcatca
     5401 atgttccaca cttttgatta gctcaattga ataatcagga tgcctggtct ttttcttttt
     5461 ggcattcatc cagctctttc ttcagcgcct ttttatcctg gtacgatgca atcagcatga
     5521 tcgggatcag cagcggagct acaataattc ccagggcaat atctttcagt ttcggcatga
     5581 tcccctccct tagtcattat caaaaacagc attcagcaca cggccaaata agccacgttt
     5641 tttgtgttgt ggcactgcca cattttgcaa cgtggcatgc tgtagcgttt gggtgtggta
     5701 tgccgtattt tcttgctgtg gcattgccac attttctgca atatttaatt catgattctt
     5761 tttattatca ttgtcttgtt gtggtgtcgc atgctgttgc atgtcagtgt cgcatgccgt
     5821 agcatcttgt tgtggtgatg ctgtggcagg tgcaacactt ttcagctcaa tgagtgtttg
     5881 catacggtca atttgttgca ttaaccgttc ttcacgttct tggtattttt tgacttcatt
     5941 ttctaaatgg gcaataagtt tttttgtaga ttcatcacct tcttgcttag atgattcaat
     6001 tttttggggt gtttgctcag gttctccaaa gcatcggatg acctctgcta gatcgaattg
     6061 tccatcactg gagcgtgata aacgtccatt acgtacagct ctgtaaatgg ttgatctatc
     6121 cacatggaat tttttagata cttcacttag tgataactta gccatttacg gtgtcctttt
     6181 tatttgatgg catatatccc acttttttta ggtatgggta tagttctttt attcggtcag
     6241 ggtcttgcag catctcagca atacgaatag caaattgcgg atagctttca gttccctggg
     6301 aataacgacc catttcagga agttcagaaa gtttatttgc aaacagatgg cgttgtgcat
     6361 cagtcatctt tgtaaagaga tctaacgtat tcggatctct ttctatcggt gggttgtcct
     6421 tctttttctg tttaaaagta aaagagaatc ctgaaattga tcgacctctt ttgtgttgct
     6481 catacttcac agtgatatct gtatgttcgt taatttgttt aatagcaagc tcaagtacac
     6541 tagttttaaa gcgttccata cgcttgtact ctgtatcgag tacgccaatt ctttgacgga
     6601 aatcacttac ctctataata ggagtagagc cagtacttct ccaagcaatt aataattcat
     6661 ataagcgaac agcataagca ctgctgagat tactaacttg ctgcaattcg tatttagtga
     6721 aatgctcttc taagcgtgta attaagggaa ctatggctgg ggcaaagata agtttaacca
     6781 ctgcttcatt atctacatat ccaatttcac taacccaacg ggatctatag ttctcaatat
     6841 tccctcgttc atttattttt tgatagctaa attgtcttgc aaataaatca ttacaggcat
     6901 cttttaatgc ttgataagcc gtattgcgat gtacgccaaa ttgattgata tagccttctg
     6961 catgtacttc aaggggatca ttagcattcc tacctttccc gctttctctt gcctcaacaa
     7021 tggccaataa aattaaacgt tgttctacta aatctaagtt atagcttgca ttaattaagg
     7081 cattgtcttt tacaactaat tctctcatga agcaccagta ttatttatta tgctatgaca
     7141 caataattaa ttttagtgct ataagcaagg taaacggtgt catattgcaa ggtaaacggt
     7201 gtcatattgc aaggtaaacg gtgtcatatt gcaaggtaaa tggtgtcata tagcctttga
     7261 aagtgttgta aattaaggct ttcagcatgt ctaaaagcat ttaaaagatt aaaaataatt
     7321 aaaagcccag ggaatgaaaa attgcccttg gttttcgctt cgctcaaact ctattgaact
     7381 tcgctttcgc tcagttcgtt ggggcaattt tttggttaat acttgcgtga ctttaagaaa
     7441 aagcaaaagc aactcggaat tcgcttcgct cataaaactt ttttactcgc tacgctcgat
     7501 ccaggagcaa atttctgtat gaaacttacg ctttcttagg ctaaagagga caaaaaaatg
     7561 tttttcagag ggtctaggcg caaattttga tagttcgctc gtagacactc gctctatgca
     7621 tctatctaaa gttaaaatct ctctttattt cgttctcatt tgcgtttttt gggctgtttt
     7681 ctgtttttca ctatgagatc tatgtttttt aagtaaaggc tcttaaatcg catttgaacg
     7741 ctttctgtga agcttttcat aattaaagtt ttgggttgaa atttttaacc tggatgaatg
     7801 gacaaaaaat tgatttttta cgtttttcta aacaaggaaa taaatacttt tttccatgca
     7861 aaaccaagca ccggccctac gtcactttag ccaattttcc aatccaagat ccggcacatg
     7921 ccgggatttt tttgtcacac accacgcatc taacgatgaa acccctcaaa gccttacagg
     7981 ataaggattt cagcgatttt ttagggtctc agaaacgtgt acagaaaaat ggtctttttt
     8041 tagaataagt agttgaatta tatgtttttt atgctgtgca catgcttgta catgaaaacc
     8101 atactttttt tagataaagt attgataaat ttaataaaag tcatgtactc atagttgtac
     8161 ataaaaaaag cattatattt aaataagttt ttgttttaga atagattaat tttgtacaaa
     8221 tagatttgta cgtaatttac ttattatttt aatttaaatt attgaattat cttgatttaa
     8281 tcttgtacaa actctaattt ttataagaga tgtaatttat gggcgaaaaa gtcaaaactt
     8341 atgcagtcag actagatcca caagtagcag aattctacga tcaattggct aattcagaag
     8401 gaatcagaac cagtaaattg ttcagtaaaa ttttgactaa tgattttcaa tctattgctg
     8461 ttaagcagca ggcagatcgt attgaagatc tggtgaatcg attagaaaag cgaatagatg
     8521 attttgagga agataataat ttttctgaaa aatactatga agatttttct ggactctatt
     8581 tcatgatgtt atggacatta atgaaaaatg gggcttctaa agatgaggtt cgaatgatgc
     8641 aagctaaagg ttattcatat gcagatgata actataagag ggtaaagtag tgtcagataa
     8701 tattattcag ctttcaaagc catgtacgct ttgtgaaaat agacaaaatg tacaactttt
     8761 tgcaggattg atgctgtgtg gaagttgtca ggaaaatatc aggcttacga atccaagtat
     8821 gttctcggcc aatgatgaga ttgaacaaaa agcccaggat taacctgggc ttcgcataag
     8881 agattttatg ttaaatgccg aactgaatta aaaatagaaa tgtagttgag ctgatgcttg
     8941 ctcatttctt tatttttagt aatt
//
