LOCUS       NC_019159              13636 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae plasmid ColEST258, complete sequence.
ACCESSION   NC_019159
VERSION     NC_019159.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226135
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Garcia-Fernandez,A., Villa,L., Carta,C., Venditti,C., Giordano,A.,
            Venditti,M., Mancini,C. and Carattoli,A.
  TITLE     Klebsiella pneumoniae ST258 producing KPC-3 identified in italy
            carries novel plasmids and OmpK36/OmpK35 porin variants
  JOURNAL   Antimicrob Agents Chemother 56 (4), 2143-2145 (2012)
   PUBMED   22252815
REFERENCE   2  (bases 1 to 13636)
  AUTHORS   Villa,L., Carta,C. and Carattoli,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2011) Infectious diseases, Istituto Superiore di
            Sanita, Viale Regina Elena 299, Rome 00161, Italy
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 05:14:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 17
            CDSs (total)                      :: 17
            Genes (coding)                    :: 13
            CDSs (with protein)               :: 13
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JN247853.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13636
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:573"
                     /plasmid="ColEST258"
     gene            <5..175
                     /locus_tag="HS783_RS00005"
                     /pseudo=""
     CDS             <5..175
                     /locus_tag="HS783_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /name="serine hydrolase"
     gene            <323..907
                     /locus_tag="HS783_RS00010"
                     /pseudo=""
     CDS             <323..907
                     /locus_tag="HS783_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            917..1174
                     /locus_tag="HS783_RS00015"
     CDS             917..1174
                     /locus_tag="HS783_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            1259..1408
                     /locus_tag="HS783_RS00020"
     CDS             1259..1408
                     /locus_tag="HS783_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            2889..4853
                     /locus_tag="HS783_RS00025"
                     /old_locus_tag="D647_p8001"
     CDS             2889..4853
                     /locus_tag="HS783_RS00025"
                     /old_locus_tag="D647_p8001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            4853..5584
                     /locus_tag="HS783_RS00030"
                     /old_locus_tag="D647_p8002"
     CDS             4853..5584
                     /locus_tag="HS783_RS00030"
                     /old_locus_tag="D647_p8002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            5591..6121
                     /locus_tag="HS783_RS00035"
                     /old_locus_tag="D647_p8004"
     CDS             5591..6121
                     /locus_tag="HS783_RS00035"
                     /old_locus_tag="D647_p8004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6149..6328)
                     /locus_tag="HS783_RS00040"
     CDS             complement(6149..6328)
                     /locus_tag="HS783_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6397..6774
                     /locus_tag="HS783_RS00045"
     CDS             6397..6774
                     /locus_tag="HS783_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(6836..7135)
                     /locus_tag="HS783_RS00050"
                     /old_locus_tag="D647_p8006"
     CDS             complement(6836..7135)
                     /locus_tag="HS783_RS00050"
                     /old_locus_tag="D647_p8006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7125..7388)
                     /locus_tag="HS783_RS00055"
     CDS             complement(7125..7388)
                     /locus_tag="HS783_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(7694..>7984)
                     /locus_tag="HS783_RS00060"
                     /pseudo=""
     CDS             complement(7694..>7984)
                     /locus_tag="HS783_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /name="Exc2 family lipoprotein"
     gene            complement(8019..11024)
                     /locus_tag="HS783_RS00065"
                     /old_locus_tag="D647_p8008"
     CDS             complement(8019..11024)
                     /locus_tag="HS783_RS00065"
                     /old_locus_tag="D647_p8008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143775.1"
                     /translation="MPVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            11186..11743
                     /locus_tag="HS783_RS00070"
                     /old_locus_tag="D647_p8010"
     CDS             11186..11743
                     /locus_tag="HS783_RS00070"
                     /old_locus_tag="D647_p8010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001217881.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            11977..12531
                     /gene="aac(6')-Ib"
                     /locus_tag="HS783_RS00075"
                     /old_locus_tag="D647_p8011"
     ARG             11978..12528
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            12601..>12816
                     /locus_tag="HS783_RS00080"
                     /pseudo=""
     CDS             12601..>12816
                     /locus_tag="HS783_RS00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing protein"
                     /name="nucleotidyltransferase domain-containing protein"
     gene            12882..13586
                     /locus_tag="HS783_RS00085"
                     /old_locus_tag="D647_p8012"
     CDS             12882..13586
                     /locus_tag="HS783_RS00085"
                     /old_locus_tag="D647_p8012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_004217321.1"
                     /translation="MNPFKGRHFQRDIIRWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     ARG             12614..12870
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.14"
                     /coverage="32.32"
     ARG             11927..12531
                     /name="AAC(6')-Ib10"
                     /gene="AAC(6')-Ib10"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.97"
                     /coverage="99.01"
     ARG             3..175
                     /name="TEM-232"
                     /gene="TEM-232"
                     /gene_name="TEM beta-lactamase"
                     /tool="rgi"
                     /drug_class="monobactam; cephalosporin; penam; penem"
                     /identity="100.0"
                     /coverage="19.93"
     Typing          complement(1512..2090)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4854..5581
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.34e-150"
ORIGIN
        1 ccctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
       61 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
      121 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
      181 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
      241 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
      301 gttccactga gcgtcagacc ccgaagccga taaaaaggta aaagaggcag aggcaaataa
      361 agatgatttt gtgacttata acccgcctca tgaatatggc tccgggtggc aggatcaggt
      421 tcgctatctt gataaggata ttcagaatca gaatgagaaa ttaaaagctg cccagacatc
      481 tttaaacgaa atgaatgaat ccttatccag ggataaggct gcgctttccg gggcgatgga
      541 gagccggaaa caaaaggaga aaaaagcgaa ggatgcagaa aataaattaa atgaggaaaa
      601 gaaaaaacct cgtaagggaa ctaaagatta cggccatgat tattttcctg atcccaagac
      661 tgaagatatt aaggggttgg gagagttgaa agagggtaaa cctaaaaccc ctaaacaagg
      721 tggtggtggt aagcgggccc gatggtatgg tgataaaaag cgtaaaattt atgaatggga
      781 ttcccagcac ggtgagcttg aagggtaccg cgccagtgat ggcgaacacc tcggggcatt
      841 cgatccaaaa acgggtaagc aggttaaagg gccggatcca aaacgaaaca ttaaaaaata
      901 tctttaagag gtaaatatgg ggcttaaatt acatattcat tggtttgata agaaaaccga
      961 agagtttaaa ggtggtgaat actcaaaaga cttcggtgat gatggttctg tcattgaaag
     1021 tctggggatg cctttaaagg ataatattaa taatggttgg tttgatgttg aaaaatcatg
     1081 ggtttcgata ttacagccac actttaaaaa tgtaatcgat attagtaaat ttgattactt
     1141 tgtatccttt gtttaccggg atggtaactg gtaagcaaaa gttatcaagg atgatttgaa
     1201 atacagactt ccggtgttca gggatgaata ccggggtttt taaatacagg gaaggtatat
     1261 gaaaaaggta aaggttgttt ttctttttat attgattttt tcaggctttc ttctggtcgc
     1321 atgtcaggca aactatatcc gggatgttca gggtggaacg gtggcaccat cgtcctcctc
     1381 tgaactgacg gggatcgcgg ttcagtagaa aagatcaaag gatcttcttg agatcctttt
     1441 tttctgcgcg taatcttttg ccctgtaaac gaaaaaacca cctggggagg tggtttgatc
     1501 gaaggttaag tcagttgggg aactgcttaa ccgtggtaac tggctttcgc agagcacagc
     1561 aaccaaatct gtccttccag tgtagccgga ctttggcgca cacttcaaga gcaaccgcgt
     1621 gtttagctaa acaaatcctc tgcgaactcc cagttaccaa tggctgctgc cagtggcgtt
     1681 ttgcgtgctt ttccgggttg gactcaagtg aacagttacc ggataaggcg cagcagtcgg
     1741 gctgaacggg gggttcttgc ttacagccca gcttggagcg aacgacctac accgagccga
     1801 gataccagag tgtgagctat gagaaagcgc cacacttccc gcaagggaga aaggcggaac
     1861 aggtatccgg gaaacggcag ggttggaaca ggagagcgca agagggagcg attcatcgga
     1921 aacggtgatg atctttaagt cctgtcgggt ttcgccactc ctgactgatt catggttgag
     1981 ccaccggctc ccacagatgc accgaaaaag cgtctgttta tgtgaactca gtcaggaggg
     2041 cggagcctat ggaaaaacgc cacctgcgtg gccttgccgt tttgcctcac atgttagtcc
     2101 cctgcttatt cacggaatct gtgggtaact ttgcatgtgt ccgcagcgcc cgccgcagtc
     2161 tcacggccgg agcgtagcga ccgagtgagc gaggaggcgc aatttccccg gcacctacaa
     2221 gacggacacc ggacgcgctg atgtggcata ttgtcccctg attaagtctt tatattccct
     2281 gataataaat taccaggata ggtgaatcat atcggttttt cgctgaacat tttacgttaa
     2341 cctatctctg ttgctttttt ctccttacgg agaaatacag gtcatccccg cgagggcgtg
     2401 gctgacctca gggggatatc ccctgcttac gctggctgga cgccaccgac gcacggtctc
     2461 tccccctctt tctggctccg tccggctgga cgccggccac gccagaaatt cacccggccc
     2521 ccgtcaggct cacgcctgac cacccacaaa aatccacggt gctggacgca cctggcgaag
     2581 cagcaaagct gctcctgatt ttgcgggtgg tcagccgctc gctatggcga actctgacgg
     2641 gggccggaac accggaaaag accgcactgg ggtgccgcct tttcctctca cacgctgggg
     2701 cgtgctcgtt gttcctccgg ggctgcgcct tttccgcatg gggcggaaag aggctggcct
     2761 ccgtggtgaa agctgaacgc aatcaccacg gacgtgaccg ccgccgccat gccttggggc
     2821 atgtccgcgg cttgtgcagc gggggctgca catcacgccg gcaaaccatc gtctgaagga
     2881 gagagaacat gtttaatacg gattcgcttg cctggaagtg gcacattccg gagctggccc
     2941 ggcagattta tccgtgggtc agctggctgt accaggagta ttacggtctg gccggggctt
     3001 tcctagttat cggcctgttt ttcggtctgg ctgaactttg ggccggacgg atgcgttttg
     3061 tggggctgag tcataaggag cgacgggagt atgccgatcg ctataagcgc gataataaac
     3121 ggccagcgcc attttcggtg ctggcctcgc tggattcgcc cgggcggatg gtcagggcac
     3181 gtcgacatgt ctggaactac gcgtactggc tgaccggcgc ggccggtctg gcccttctgg
     3241 gggggctggt tgtcgccgtg tttatcgaga gctggggaga aaccgtcctg cgtccggacc
     3301 tctacgcaaa cgcagattat tacctgctgc agccggctaa cctgttcggg attgcctggt
     3361 gcgcgggcgg gctggctgtc gggattgtgt tggcgcggct gctggcgttc ctgctgcttg
     3421 acggtcattt tgcggctgct gacgaggcgg tgaacgcgaa actgaatcag gagagccggc
     3481 gcagcagcca gcggacagga gagatgaccg acgtgcgcca cctgcatttc ggggagccgg
     3541 taccggttaa tgcgctggcc gatttttcct cggagcaggc ccggcaggag caggcggtgt
     3601 ttctgggtaa ggatgagcag ggcaagccgg tgctggttcc gcgtgatacc tggcgcaaga
     3661 ccaacattca gatcctcggc ctgcccggga gcggcaagag cgtgatggcc accaacgcgc
     3721 tcattcgctg cgtacgcgat ttcggggacg cggtggtgta cttcgacccc aaaggggacg
     3781 catgggcgcc gcacgtcttc cgggcgcact gtccggactt caccctgctg gatctgcggc
     3841 cggggaaacc ggcgcagctg aatctgttcc gcgatctcga ccagtacgcg ctgaaaaacc
     3901 tgctgattgc cgggcttaac ctgggcgaga ccggcgacgt ggcggaccac taccgcatca
     3961 gcgagcagaa agcggcgaag ctgattgcgg agcagtttcc gcagggggca aacattcagc
     4021 aggtgctggc ggcggcgtac gcgctgccgg aggccctgaa aaaggacgtg aaggggttga
     4081 tcactaggct ggaaaacgtc gctgacctga gcgtcctgca gacggacagt ggcatcgatg
     4141 tggcggggat tatcaacggc ggcggctgcc tgtacgttat cggctcgatg gacgacgagg
     4201 cggtgatccg tgtgcaaaaa atgctgtttg cccgctgcgc gcaaatcatc attgcgcggg
     4261 acgagttccg ggagtggcca cacgtcagcg tgatgctcga tgaaattaaa tatctgctgt
     4321 cgaagtatgt actgaacgca ctggggacgc tgcgcagccg ggactgcaat ctgttgctcg
     4381 cgcaccagtc gctgggcgat ttcgggcagt gcgggcagga tctgcccgct gaatttgtga
     4441 aaaccacggt actggataac acgccgatcc gctggtttta ccgggcggcc agcctggagt
     4501 ccgcgcagtg ggcggcgggg cagaccgggg aaatcagggt ggacgtggag cgccgccgtg
     4561 ccagccggga ggcggggaac gtggagcata tcagcgggga cagcttcatc cagaaggagg
     4621 cgcggccgct gtttgacgtg aacaccctgc aacacctgcc ggacgggttc gcggtcatga
     4681 ccgggctggg tgtggcccgg atggcgttca gcagtccgct gcgggttgag cgccgggaaa
     4741 tcccgttgca gcgtttcccg gtgctggcga aaaccgatcc cctggcggag ttccggcccc
     4801 cggccgacgg cggcgggccg aaagcggatg atgattttgc ggggatttac tgatggcgct
     4861 ggagctgtac aacgtcagcc acgcgaaacg acaggcgcgg aacgcggaga aaacccggct
     4921 gaccctgcgc tggttgcggg aagagctgtg ttccacggcg gagctggttg cgcggcggct
     4981 ggggattgcg gcggtgcagc cggtctaccg cttcctcgac agcctggtgg cgaaagggct
     5041 gctggtccgg accaaatatc cggtggacgg ccgtcaggtc agcgtgtggg ggctgacgcc
     5101 gcatggcgtg gcgttctcgt tcgacgagga cgaaccgctg gcggatgtta ttccgtttca
     5161 gccgtcaagg gtgtcggcag cacatctgcc gcaccggctg gccgtgcaat ccctgcgcct
     5221 tgatatggaa gccagagggg ccaccggctg gcgcattctg caccgtctga gtcttaaagg
     5281 catgaaagtg ccggacgccc tggcggagct ggacggtaaa acggtggcgt ttgaggtgga
     5341 gcgcaccgtc aagagccgtc gccggtatca ggaagtggtg tccgggtatc tgttcaaccg
     5401 caaagctaac ggcatcgatg aaatctggta catctgcccg gaccgggcga cgcaggtcag
     5461 ggtgcagcgg gcaatcctgt cggtggatga aatcgtgaac ccacagaccg gcgaggcccg
     5521 caaaacggca gagctggatc gtgaacggtt gtttgcgtgc tttaaattta tgacaacgga
     5581 gtgagggatt atggcgctgt acatggtatt tattgaagac gcggaggacc ggaacggcga
     5641 gcgctgcatc atgccgacca gcgagcatct ggaagatatg gtggtgctcg cctggcagac
     5701 cggggcgtat gttttctgtt ttgtcgaccg ggcccagcat ctggcgatga tgaaatccat
     5761 cgggatgtat gacgagcggc tcaacagcga cgaaaaagcc acgtactttt tcactggtaa
     5821 aaatccgcaa tgggaacgga acaaacccac aggaatgaag ctgtgtgatt acctctatcg
     5881 caaggtggag cggttcgcca tcgagaacgg tgtgcaggtg gttgatgccg ggaagtgctc
     5941 accggagcag attaacgcga tgattgacgt ggtgagtgag cggcacgggg aaagcgcggt
     6001 gaacgagaag ctgaaagcgg agctgatgcg ggaatataac agccgctatt ttaacgtgcc
     6061 ggtacctgaa ctgccggagg acaaacctgc ccgccggtgg tggccgttcg gcaaaaaata
     6121 aatgccagca ccggccatgc cggtgctgtt attcctcccc gagcttatcc cgcatcacct
     6181 gccagagtgt ttccgcatcc tcgtgtagcc gctcgcacat gtcggccggg gtatccatat
     6241 ccagctcatt cagtttatcg agcaatagca gcgactgctc cttaataaat ttagccatat
     6301 tgagggctgt tttttcctgt tttgtcatga ttctttcctt ccgggcatgg tgtgcgcctg
     6361 tcaggtatac tgtctcaaaa atagcggggt gagacaatgg cgtgggtgac ggtgagacag
     6421 gcgacggaac tgacgggtaa agcccgaagt tccctgtatc gggatatggc gaaaggccgc
     6481 gtatcatacc gtacggaagc tgatggcggc cgggtggtgg ataccagcga gcttatccgg
     6541 gtgtacggtg agttaagaca ggatgagact cacgagcggg acgagttgag actgtcgggt
     6601 gagactgaaa aaatggcctc cgacgcgttg atcgcggaga taaaagcact cagggaggag
     6661 gtggccgggc tacggcagga aatgcagtcc atgagactgc tggaacacaa accggagaca
     6721 gcagagaccc cggccggaac accgcgccgg tggtggccgt ggggaaaacg ctgacgcgct
     6781 gtgcttgtcc aaccggcgac ggccacgcaa tgaattgcgt ccccgtcacc ggttattaga
     6841 gaacaatatg gggattctgg cgctggccga ccacggccag aacccggact tttctggtct
     6901 ggtcatcaac atcatagagg acatgctgtc ccatcagact gatccggcga acgtcctcgc
     6961 catagcgtga gtccacttca tagagtcgcg ggaagtcggc cagtttgtcc gccgcagcga
     7021 gaacccggtc ggtaacgttc tgcgcatccc acagggatga attttccgcg atatagtcaa
     7081 acagcgtcgt tacatcctcg atggctccct gcttccattc cacgtcatac ggcatcgcgc
     7141 agttcctgat aacggccatg caggcgctgg cggagttgtt ccgtggtcat acccggacgc
     7201 gtgtcagcac gggcggcggt cactttggcg cgaacctgat cttcatactc tgcctgggcc
     7261 agttcttcgt tagccagtcg ctgcaacagc gcagctttgt ccgggccacc ttccggcagt
     7321 atgcggggca ggctttcagc cagttcccga agaaagttgg cgttttcgta gtgtgtagtg
     7381 ttgctcattg cggagcctcc cgggttaatg cggcaatatg aaggaagttt agcagggggc
     7441 ggtctgaaag aatatggtcg tgtcagtcag gctgatgaag cagaaagtcg gtaaaaaagg
     7501 gcttacggca tccattttac gtcaaaacat atgccatgca gttgcgtgca taggcatgca
     7561 ttaggataaa atttatcggg cgcgtttccg gcagttttcc gggggggttg ttgcctgttt
     7621 tatcccgtag ccgccggaaa cgccctgagc ccgtctgagc ggtgcgcgta atgacgcgtt
     7681 atggtaaatc cacttactgt ctgccttcat agccatcgag ataaacagct gaaatggtgt
     7741 cagccagtag ccttgatttt ttcggtgtga ggtctttgct gcggtaatcg gtacccgcaa
     7801 attgtgttgt gccttggagt gagtttaaaa attcctgact gtgaaactgc tgaatgcgtt
     7861 gctgcacatc actccgtgat tttccggtcg ctttatcctt tgcgcccata tcccagaact
     7921 gccggaaaaa aggcaccatc attcgtgttg tgtcagcttt ctgggtggaa aaattgggat
     7981 caaaaggggt ctgacgctca gtggaacgaa aactcacgtt aagcaacgtt ttctgcctct
     8041 gacgcctctt ttaatggtct cagatgacct ttggtcacca gttctgccag cgtgaaggaa
     8101 taatggccga gcatattgat atgtccgtgg caaagcgggg agaggcgtgc gatatcttca
     8161 tcattcagtg tttcaccctg cgcccggaga tgatccagag ctgcctgcat ataaatagtg
     8221 ttccataaca cgacggcgtt agtgaccagc cccagagctc ccaactgatc ttcctgaccg
     8281 tcggtatatc gttttcttat ctcacctttt tgaccgtgac agatggctct ggcaactgca
     8341 tgacggcttt ctccccgatt aagctgggtc agaatgcgcc ggcggtaatc ttcatcatca
     8401 atataattaa gcagatacag cgttttgttg atgcgcccca cttcaatgat tgcctgagtc
     8461 agtccggagg gacgttcact tttcagcaat gaacggacca gcactgaggc ctgtactttg
     8521 cccagcttca gggagcctgc ggtccggatc atttcgtccc actgaaggac tatttttcgg
     8581 ggatctgatt gccctctggc aatatcattc agcacgccat agtcggcatc atggtccatt
     8641 cgccagaaaa ccgaagcacc ggcatcagcc aggcgtggag aaaactggta tcccagcagc
     8701 cagaaaaggc caaagacaag atcgctggca cctgccgtat cggtcataat ttcggttgga
     8761 ttcagcccgg tctcctgttc cagaaggcct tccagcacaa agatagagtc cctcagcgtc
     8821 cccggtataa cgatgccatg aaagccggaa tactgatcgg acacaaagtt gtaccaggtg
     8881 atccctctgt tattaccaaa gtatttgcgg ttcggtccgg cattgattgt tctgactggc
     8941 gtaacaaagc gcattccatc tgcagatgcc acttctcctc caccccatat ctgtgccagt
     9001 ggcagcgttg cctgaaaatc aaccagtctg gcattagcgc tggtgatagt ttcagcccgc
     9061 agatagttcg cttttgtcca gttcagccgg tgtcgggtca gtgcaggaac atttgatctg
     9121 atcagtggtt ccagaccgat attgcaggct tcagccatca gcacggcgct gatgctgacg
     9181 ggcagatcat caactctggc actggcttca ctggcatgga aaaactcatc agcaaatccg
     9241 gtatgggcgt taatttcgag cagcaactcc gttaaatcca ccggagggag cagatcactg
     9301 atcattttgc tcagtcgttt cagactgtcc ggctcatcaa gactggcgag gggagaaatt
     9361 gtcaaccggg gcttcgggcc agaaacatcg agttcgacag cctcattttc gccaagacgt
     9421 gcagcaacct gtctgtaacg actatcaagc tgatggccca gagattttat tgcttcctgc
     9481 gggtctgtcg ggtgccccaa agaacgataa accttaatcc gatttgcctg ccagtcagca
     9541 ccctgtagta atcttgcacg aggatctccc caccggttac tgccggtaac gtagacatcc
     9601 ctccgtctca gactatcctg cagtttactg agaaagcaga gcgtgtatcc ccttcgggtg
     9661 atatgttttt ccttgttaat caccagccgt ttccatgact gactgatgat ttccgttggt
     9721 gcgtcgtcaa aaaactgccg ccgtgagctg aactcccggc tgaggtagtc acaggcattc
     9781 agagtggtaa ccccggcagg tgcggatgaa aatttaacgg tattcagcag atggggcagg
     9841 aaacgacgaa cgcgcccgta ctgctccacc atttcgtcat gaaaattatc gtctgagggc
     9901 cgggcaattt cacggacaag cgtgatgatt tcagccagct tttgcctagg gatgtagctg
     9961 aacacctcag cacgaatcga ttcgtccggt gtttcttctt tcagcaagta cgaacatgcg
    10021 ctggcgagcg ccaatgcaga tttatccaga tccttcagcg agcggagccg ttttttctgc
    10081 ccaatctttc tggcgtcacg gatgataacg gccagcatgg cgtccagaac gtccagtgca
    10141 tcatccagcg ccagcgtttc ccatgcaagg acaaaggcaa ccagaaccgc catccttttc
    10201 tgcggtgaca tcctggcaat attgaacacc gaagtcatac cagcataacg tgcgagattt
    10261 ttcaggcgca cagccgggag tgtactcagg ttttcagcat gcaggccaaa atcgttcaga
    10321 gttttccagc gttcaattgc ttcattaaac gccggaccac tgatggtcac agggcctttt
    10381 ttcagtgatt ccagtaaaga caggcggctg caatcagttg gccccagcag catctccagc
    10441 tgtgaacgct gttcggctga cggtatcagt gccagtttgt tccacaggcg caacgtcgcc
    10501 ttttccctta cctctgaaat caaccgggtc agcgtggtgg ctccggggag aataatacga
    10561 tgttgcataa gccaccctgt cgccaggtcg aaaagcaggc caggacgttc gttgcttatc
    10621 cagctccggg tatataaaag acgggtaagg cgaaatgtcc agggccaggc aaattcacga
    10681 tactgatagt gctgacgtat cagcgctgca tgctcacggc gggtattttc cctctgaccg
    10741 tattctgcaa gaacggtgat atcacgaatc ccgagctgtc tggcggtaaa atgccggacg
    10801 ccggaaggaa tatgattcat gtcagtaaga aaagtgccca gaaaacgcac acacccgatt
    10861 tgcagggcaa taccaagtcg attgtgatca ccccggcttt tcccgataaa ttctttatcc
    10921 gcttcatcaa gatgaaaata acgcgccagc tgaagttcat cgggttcgcc agtgaacctg
    10981 ccataactct caacctgctc agtggtcaaa aaatcaacgg gcatatcggc ctccctgcct
    11041 gacggctttt ttaacacaac tgcaaccgtt cgaaatatta taaattatca gacatagtaa
    11101 aacggcttcg tttgagtgtc cattaaatcg tcattttggc ataatagaca catcgtgtct
    11161 gatattcgat ttaaggtaca tttttatgcg aatttttggt tatgcgcggg tctcaaccag
    11221 ccagcagtcc ctcgatattc agatcagagc gctcaaagat gcaggggtaa aagctaaccg
    11281 catctttacc gacaaggcat ccggcagttc aacagatcgg gaagggctgg atttgctgag
    11341 gatgaaggtg gaggaaggtg atgtcattct ggtgaagaag ctcgaccgtc ttggccgcga
    11401 caccgccgac atgatccaac tgataaaaga gtttgatgct cagggtgtag cggttcggtt
    11461 tattgacgac gggatcagta ccgacggtga tatggggcaa atggtggtca ccatcctgtc
    11521 ggctgtggca caggctgaac gccggaggat cctagagcgc acgaatgagg gccgacagga
    11581 agcaaagctg aaaggaatca aatttggccg caggcgtacc gtggacagga acgtcgtgct
    11641 gacgcttcat cagaagggca ctggtgcaac ggaaattgct catcagctca gtattgcccg
    11701 ctccacggtt tataaaattc ttgaagacga aagggcctcg tgatacgcct atttttatag
    11761 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaaagtg
    11821 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
    11881 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
    11941 ttccaaacaa agttaggcat cacaaagtac agcatcgtga ccaacagcaa cgattccgtc
    12001 acactgcgcc tcatgactga gcatgacctt gcgatgctct atgagtggct aaatcgatct
    12061 catatcgtcg agtggtgggg cggagaagaa gcacgcccga cacttgctga cgtacaggaa
    12121 cagtacttgc caagcgtttt agcgcaagag tccgtcactc catacattgc aatgctgaat
    12181 ggagagccga ttgggtatgc ccagtcgtac gttgctcttg gaagcgggga cggatggtgg
    12241 gaagaagaaa ccgatccagg agtacgcgga atagaccagt tactggcgaa tgcatcacaa
    12301 ctgggcaaag gcttgggaac caagctggtt cgagctctgg ttgagttgct gttcaatgat
    12361 cccgaggtca ccaagatcca aacggacccg tcgccgagca acttgcgagc gatccgatgc
    12421 tacgagaaag cggggtttga gaggcaaggt accgtaacca ccccagatgg tccagccgtg
    12481 tacatggttc aaacacgcca ggcattcgag cgaacacgca gtgttgccta acccttccat
    12541 cgagggggac gtccaagggc tggcgccctt ggccgcccct catgtcaaac gttaaacatc
    12601 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
    12661 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
    12721 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
    12781 acaacgcggc gagctttgat caacgacctt ttggaaacgg cactgttgca aatagtcggt
    12841 ggtgataaac ttatcatccc cttttgctga tggagctgca catgaaccca ttcaaaggcc
    12901 ggcattttca gcgtgacatc attcggtggg ccgtacgctg gtactgcaaa tacggcatca
    12961 gttaccgtga gctgcaggag atgctggctg aacgcggagt gaatgtcgat cactccacga
    13021 tttaccgctg ggttcagcgt tatgcgcctg aaatggaaaa acggctgcgc tggtactggc
    13081 gtaacccttc cgatctttgc ccgtggcaca tggatgaaac ctacgtgaag gtcaatggcc
    13141 gctgggcgta tctgtaccgg gccgtcgaca gccggggccg cactgtcgat ttttatctct
    13201 cctcccgtcg taacagcaaa gctgcatacc ggtttctggg taaaatcctc aacaacgtga
    13261 agaagtggca gatcccgcga ttcatcaaca cggataaagc gcccgcctat ggtcgcgcgc
    13321 ttgctctgct caaacgcgaa ggccggtgcc cgtctgacgt tgaacaccga cagattaagt
    13381 accggaacaa cgtgattgaa tgcgatcatg gcaaactgaa acggataatc ggcgccacgc
    13441 tgggatttaa atccatgaag acggcttacg ccaccatcaa aggtattgag gtgatgcgtg
    13501 cactacgcaa aggccaggcc tcagcatttt attatggtga tcccctgggc gaaatgcgcc
    13561 tggtaagcag agtttttgaa atgtaaggcc tttgaataag acaaaaggct gcctcatcgc
    13621 taactttgca acagtg
//
