LOCUS       NC_019113              10047 bp    DNA     circular CON 30-JAN-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Typhimurium plasmid
            pQnrS1-cp17s, complete sequence.
ACCESSION   NC_019113
VERSION     NC_019113.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226128
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Typhimurium (Salmonella
            typhimurium)
  ORGANISM  Salmonella enterica subsp. enterica serovar Typhimurium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Akiyama,T. and Khan,A.A.
  TITLE     Isolation and characterization of small qnrS1-carrying plasmids from
            imported seafood isolates of Salmonella enterica that are highly
            similar to plasmids of clinical isolates
  JOURNAL   FEMS Immunol Med Microbiol 64 (3), 429-432 (2012)
   PUBMED   22151215
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Akiyama,T. and Khan,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2011) Division of Microbiology, The National
            Center for Toxicological Research, 3900 NCTR Rd, Jefferson, AR
            72079, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 05:08:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 14
            CDSs (total)                      :: 14
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JN393220.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Salmonella enterica subsp. enterica serovar
                     Typhimurium"
                     /mol_type="genomic DNA"
                     /strain="484"
                     /serovar="Typhimurium"
                     /isolation_source="Frozen yellow eel chunk"
                     /sub_species="enterica"
                     /db_xref="taxon:90371"
                     /plasmid="pQnrS1-cp17s"
                     /geo_loc_name="Viet Nam"
                     /PCR_primers="fwd_name: qnrs-a2, fwd_seq:
                     agtgatctcaccttcaccgc, rev_name: qnrs-b2, rev_seq:
                     caggctgcaattttgatacc"
                     /note="B"
     gene            complement(join(8680..10047,1..9))
                     /locus_tag="HS824_RS00005"
                     /old_locus_tag="D645_p7011"
     CDS             complement(join(8680..10047,1..9))
                     /locus_tag="HS824_RS00005"
                     /old_locus_tag="D645_p7011"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900944.1"
                     /GO_function="GO:0009975 - cyclase activity [Evidence IEA]"
                     /GO_process="GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="WP_080196049.1"
                     /translation="MVNVEFFNYIECIFMRIKKPHLRPIVITLSVGGILLTSIFLIVVI
                     MVFKRENIEKDLLDANSSYAMKMSDVMSNYIEIAQRELAYGAKKIESTNDIDSLKNEAD
                     RLRLQSGMFNSVLVVSDGAVVLATSPESLGLVGVHLNSSASKLAIEAKKPFISAPYKSA
                     SGNLIIFLSHPIYDKNSNYIGYVGGSIYLKEQSLFSDVLSRHFFKSDTEISIVSDDGNV
                     IFNNDNSAVGRPMVMDDNLKSKLTSSERGEGIFFSSGNKYLLGYAHMKNTAWSVLVYSH
                     ADSVTTILITYAKSVLVLLIVIVITFSLFSYFIASRISIPLEKLAISTNAKDIGGSLVD
                     IKGINAWYVEADRLKKALFTHIKIMIERVDSLNEVALKDPLTGVNNRLGFSNKIQNHKQ
                     GSGASVIAIDVDFFKKINDNFGHGVGDEVLVTLAQVINCGFPGLGRFPQSRGQPERKPE
                     "
                     /name="diguanylate cyclase"
     gene            complement(315..530)
                     /locus_tag="HS824_RS00010"
                     /old_locus_tag="D645_p7002"
     CDS             complement(315..530)
                     /locus_tag="HS824_RS00010"
                     /old_locus_tag="D645_p7002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137421.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(534..1019)
                     /locus_tag="HS824_RS00015"
                     /old_locus_tag="D645_p7003"
     CDS             complement(534..1019)
                     /locus_tag="HS824_RS00015"
                     /old_locus_tag="D645_p7003"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012482081.1"
                     /translation="MSSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEQSVRAELNESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSVLLIASSAGILWWQSQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRSCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1054..1758)
                     /locus_tag="HS824_RS00080"
                     /pseudo=""
     CDS             complement(<1054..1758)
                     /locus_tag="HS824_RS00080"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1697..2020)
                     /locus_tag="HS824_RS00025"
                     /old_locus_tag="D645_p7004"
     CDS             complement(1697..2020)
                     /locus_tag="HS824_RS00025"
                     /old_locus_tag="D645_p7004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_015059464.1"
                     /translation="MLTIWVTEDEHRRLLDRCDGKQLAAWMRQTCLDEKPARAAKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2670..2828
                     /locus_tag="HS824_RS00030"
                     /old_locus_tag="D645_p7005"
     CDS             2670..2828
                     /locus_tag="HS824_RS00030"
                     /old_locus_tag="D645_p7005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001658417.1"
                     /translation="MHEPPVQSDCCASSGNFQLDSNPERHDKAPLAAAIGNWELVDLDI
                     ESLEVVA"
                     /name="hypothetical protein"
     gene            complement(3071..3376)
                     /locus_tag="HS824_RS00035"
     CDS             complement(3071..3376)
                     /locus_tag="HS824_RS00035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA];
                     GO:0046872 - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="WP_223229636.1"
                     /translation="MPKPDNSCCRSEDIVCRFGGEEFVVFLPNTSVVTAECVAERIRSV
                     IESTVFPNNLRVTVSAGVATQEDPLGGIDFLLKNADDALYQAKGEGRNKVIVYSAI"
                     /name="GGDEF domain-containing protein"
     gene            complement(<3362..4392)
                     /locus_tag="HS824_RS00040"
                     /pseudo=""
     CDS             complement(<3362..4392)
                     /locus_tag="HS824_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612174.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            4787..5443
                     /locus_tag="HS824_RS00045"
                     /old_locus_tag="D645_p7007"
     CDS             4787..5443
                     /locus_tag="HS824_RS00045"
                     /old_locus_tag="D645_p7007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="WP_001516695.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFVN
                     CKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRTNF
                     AHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLSRGV
                     FSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrS1"
     gene            complement(5762..6376)
                     /locus_tag="HS824_RS00050"
                     /old_locus_tag="D645_p7008"
     CDS             complement(5762..6376)
                     /locus_tag="HS824_RS00050"
                     /old_locus_tag="D645_p7008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412822.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_014839983.1"
                     /translation="MFIGYIRMSKADGSQTNDLQRDALIAAGVDAAHFYEDRASGKREE
                     RPGLTACLKALRPGDTLVVWKLDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATIDTT
                     TAAGKLVFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMASMG
                     QSETKVSTLCQELGITRQTLYRHISPVGQLRADGIKLFNRG"
                     /name="recombinase family protein"
     gene            6538..>6720
                     /locus_tag="HS824_RS00055"
                     /pseudo=""
     CDS             6538..>6720
                     /locus_tag="HS824_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     gene            6856..7401
                     /locus_tag="HS824_RS00060"
                     /old_locus_tag="D645_p7009"
     CDS             6856..7401
                     /locus_tag="HS824_RS00060"
                     /old_locus_tag="D645_p7009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="WP_001493763.1"
                     /translation="MVWRRLRIAADTSLAALHFIFQIVQGWGDDHLHQFHIYGKDYGIS
                     YEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIRVEAIYENSTLKAPFCIS
                     GHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADDLDAVRFNRHKINRQLSRL
                     DLASPVLEPEVIWLGRRR"
                     /name="plasmid pRiA4b ORF-3 family protein"
     gene            7538..8110
                     /locus_tag="HS824_RS00065"
                     /old_locus_tag="D645_p7010"
     CDS             7538..8110
                     /locus_tag="HS824_RS00065"
                     /old_locus_tag="D645_p7010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001493762.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNRP
                     GLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGTNN
                     PMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALRSRG
                     VRLRIACDHAGLGVSTYYKLRHQMNNQ"
                     /name="recombinase family protein"
     gene            8147..>8752
                     /locus_tag="HS824_RS00070"
                     /pseudo=""
     CDS             8147..>8752
                     /locus_tag="HS824_RS00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /name="ISKra4-like element ISKpn19 family transposase"
     ARG             4788..5440
                     /name="qnrS1"
                     /gene="qnrS1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrS1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2291..2837
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.652"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(212..1707)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.583"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atttaccata acatcccttg cccgcaccgc cccggacggg ttcggcgcga atgcggcgtc
       61 agcccggtaa aaccggcagt aaactccccg ctaaaccgcg ctttttgagc cagataaatt
      121 tccccaagat gcatagccat gcagccgggg ggatggcacc gcctttcggc ggggatctgc
      181 gccgaaagac ccgttttatc cggtttgtta cagcgacgga ccgttgtacg tccgctggcg
      241 ctgcgatcgt tccagctcca gcgctttccg gtgctgcgcc gcccgttcct gctcccggtg
      301 cgcctcgata ccattcagcc ttgcgacaac cggcgcagct gctcgctcaa actctgcacc
      361 tgcctgctca agcccgtgac gcgctcgctc agcgccgcgt tctcccgttg cataagacca
      421 gacatcgtcc gccattccgc gaaggcgttc tcccactcgt ccagcctttt cgagtagtcc
      481 tgctgtagct gctctaatgc gctcagcaac tgtttttcca gctccgtcat gtgctatttc
      541 cccgccagta tcatccagct cgaatcctct ccgaaccgtc ccgcttccgg gttcaccctc
      601 acgcagctgc gtctctgctc gccgcaggtc gagagctgca cgccgctgtt ccgctcggac
      661 agcatcgcct gcgtgctctt ctgctcccgg atggtcgtgt aattgtcgag tatctgctga
      721 ctctgccacc acagaatgcc cgcgctcgat gcgatcagca ggacggaaac caggacgatg
      781 gtcagccacg tctggctgac catacgcacc atgcctttcg tgcgctggct catggctgag
      841 gacagcttcc ggtcgtggtc gaggatggcg gcgctgattc tcttctcgct ttcgttcagt
      901 tccgctctga cagactgctc gtgctcgctg aatgcggctt tcagcatctc gccggtactc
      961 tgctgctgcg ctttcgattt ctgctctaag tccttcgcca gcgttaaaag actgctcata
     1021 gatggctccc tttagtcgga tgtttcgccc cccgtccggg tcggcaatgc tgatactgct
     1081 tttcgtggtg cgtaccacct caaaacccgc gttttccagc gcctcagtaa cgtcctgacg
     1141 ggttttcagc tccccggacg aggctaaggc cagcaaaccc cgggtgatcg cctgagctgc
     1201 ctcctgcttc gtttccggca gcgcggacgg cgtgaccagc gcccgccggt tttccggcgc
     1261 attcgggtcg tgcagcccca gcctgccgtt cacgatggtc tgccaggcat cgatgcgcgg
     1321 acggtcagcg cggtcgtaat acggctggag ccttttgccg gtcagcagct ccgtgttcgg
     1381 gatcaggaag ttcagctcca gccgcccctt gtcctggtgc tccacccaca gcacgctgta
     1441 ctggtcttta tcgagtccgg gcatcagaac acgctcgaag ctcgccatca gtttttcacg
     1501 ctgaccgggc ggtaagtcct gttcggcaaa cgacagcacc ccggaggtgt actttttggc
     1561 gtagggcgag gcgtcgatta gctcccggac ttcctccggt ttaccctgca gaacgctggc
     1621 gccgtcgcgc ttgcggtcat tacccagcag ataatccacc ggaccggcac cgccgccgcg
     1681 ccctcgtggg tgaaatttaa cgatcatcgt cagccccctt ttccagcaca gcgtgccgca
     1741 ggcgctcaag tccggcatca atggccatca gcgcagcgac aatctgtacg cgatcatgtc
     1801 cggtaccacc accggcgtta acctgccggg caatctgatt gaggttgttt cccataccgg
     1861 caagttgacg aagcaacgcc ggcgagatcg acggaagttt ggcggcacga gcaggtttct
     1921 cgtcgagaca cgtctgccgc atccaggccg cgagctgctt accgtcgcag cgatccagca
     1981 gacgccggtg ctcgtcttca gttacccata tcgtgagcat tttgctgcgt ttgtctgcca
     2041 atccggcctc ctgataacgc cgggacggac ggggaattat ttcccggtcg gcgcggtgtt
     2101 ggacaagccg caggcgcgtc agtgcctttt agcgggtttc ggggcgcagc cctgaaccag
     2161 tcacgtagcg ctagcggagt gtatactggc ttaaaatgta acatcgcaga aacatcaagc
     2221 atagctgcca cataaagcag gtgaaaaatg agattcgtat gcgtcagcag aagatgcact
     2281 gccggagaaa tgccgcttcc tcgctcactg actcgctccg ctcggtcgtt cggctgcggc
     2341 gagcggtacc ggcttacggg cggggcggaa atttcctgga agatgctagg aagaaactta
     2401 acagggaagc gataaggcca cggcagagcc gtttttccat aggctccgcc cccctgacaa
     2461 gcatcacaga atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagatc
     2521 ccaggcgttt ccccctggta gctccctcgt gcgctctcct gttcctgcct ttcggtttac
     2581 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg
     2641 ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgactgct
     2701 gcgcctcctc cggtaacttt caacttgatt ccaacccgga aaggcacgac aaagcgccac
     2761 tggcagcagc cattggtaac tgggaattag tggatttaga tatcgagagt cttgaagtgg
     2821 tggcctgact acggctacac tgaaaggaca gtatttggta tctgcgctcc actaaagcca
     2881 gttaccttcg gaagaaagtg ttggtagctc ttaattccgg caaacgaacc accgttggta
     2941 gcggtggttt ttttgtttac agggcatgag cttacgacga catcgaaggg ataacaagag
     3001 gatccgcttc ttgaaccctt ttccccacaa aaaaacgcca cactagcgcg taaagcgtca
     3061 tcaaaaaaac tcaaatagcg ctatatacaa tcactttatt acgcccctct cccttggctt
     3121 gataaagagc atcatcagca tttttcagca aaaaatcgat cccaccaaga ggatcctctt
     3181 gtgttgccac accagcagag acggtgaccc gcaagttatt agggaaaaca gtactttcaa
     3241 taacactcct aatacgctcc gcaacacact ccgctgttac cacagatgta ttcggcagaa
     3301 aaacaacaaa ctcttctcca ccaaaccgac aaacaatatc ttcactacgg cagcaagaat
     3361 tatccggttt cggcatgatg ctgatgtagt cccgttttat cgtcatcacg aagctttctg
     3421 ctatgccatt gctttccggg cttcgcactg ctgtcgtaca aggctccagt cccagcaacc
     3481 gggcgaacgc cggcactgtt ggcaactgcc ttccgaagcg tttttccacc gcacccagca
     3541 tgacatcctg caccgtttct ttgtcatagc ctccggtgct cgctgcccaa tcaatgatct
     3601 ccctgtcgca gcagtcctgc gcgaaggtca cccgcagctt ttcgccatta tcacagcgga
     3661 actcaaagcc atctgagcac cagcgccggt tactttccgc tactgctacg cgccccttat
     3721 gggcccgctt acggcaagga tcagcgggtt tacgttcaag aagcagatta tgcgttctca
     3781 taatgcgata cacccgcttt gcattaacca caggcaggcc gtcccgctcc gactcccgtc
     3841 gcagcagcgc ccacacacgg cgataaccat acgtcggcag gtcagccacc gccatgttta
     3901 tccgggacaa tacggtggta tcgtcagaac ggggctgccg tctgcgatcc tgtcagtcag
     3961 atggccggtg aacccgaatg ctcaactgcg cacgcgacac gccaagactt ctgcaaacgt
     4021 cgcttattcg tcgttcccag gcaacgaggg cgcatgcgca atccattttt ttgcccggcc
     4081 gaactccacg gcctctttga gtatctcggc ttccatcgtc tttttgccca gaaggcgctg
     4141 gagttcgcga atttgcttat tggcggcagc gagttcggag gccggcacca cttcctcgcc
     4201 cgatgcaaca gccgtcagtg aaccgtctta atattgcttg cgccatttga atatctggtt
     4261 tgcattgatg ccatgcaggc gcgcgacatg agacacggtc ataccgggct ccatagtctg
     4321 ctgaataatg gcaatttttt cctgcggtgt acgacgccga cgccgctcag gtcctgataa
     4381 cacttcaacc atcttatcgt tctgactggt attaaacata gttccaagac tacctcttat
     4441 tttaagagag tcgaagtgtc tggtgatcta tggggccagt ctagccctct tccaagtgta
     4501 gggagatata gtcaaagttt gcttctccgc cgatgagtcc gccctgcgtc ggatagtccg
     4561 attttttacc ggcgaagcac ggctctccta accctatcta aagcgaataa cttttttcaa
     4621 cagggccgcg tgccaatgca agagcgatag gcgctctacg cgccaatttg accacttaaa
     4681 acaggtaaat tgaggggttg taatgtgttg atgtaacagg cttttatttt aatgtcttgg
     4741 catatgtata atggtagtct agccctcctt tcaacaagga gtactcatgg aaacctacaa
     4801 tcatacatat cggcaccaca acttttcaca taaagactta agtgatctca ccttcaccgc
     4861 ttgcacattc attcgcagcg actttcgacg tgctaacttg cgtgatacga cattcgtcaa
     4921 ctgcaagttc attgaacagg gtgatatcga aggctgccac tttgatgtcg cagatcttcg
     4981 tgatgcaagt ttccaacaat gccaacttgc gatggcaaac ttcagtaatg ccaattgcta
     5041 cggtatagag ttccgtgcgt gtgatttaaa aggtgccaac ttttcccgaa caaactttgc
     5101 ccatcaagtg agtaatcgta tgtacttttg ctcagcattt atttctggat gtaatctttc
     5161 ctatgccaat atggagaggg tttgtttaga aaaatgtgag ttgtttgaaa atcgctggat
     5221 aggaacgaac ctagcgggtg catcactgaa agagtcagac ttaagtcgag gtgttttttc
     5281 cgaagatgtc tgggggcaat ttagcctaca gggtgccaat ttatgccacg ccgaactcga
     5341 cggtttagat ccccgcaaag tcgatacatc aggtatcaaa attgcagcct ggcagcaaga
     5401 actgattctc gaagcactgg gtattgttgt ttatcctgac taattgcttt gatgtgtgat
     5461 tttaaacgct caaatttata aaacgaataa tattgtcaac cttgcaataa tagacatagg
     5521 gacaaccatg tataaatttt taccctcagg cgtcttcaca atagagccat atagcagtgg
     5581 gtcacttagt gggcttaatt tttccattaa agataatatt aatattgctc agtataaaac
     5641 atcatatggt agcccatctt ggcaagtaaa cataaggctg ccatttataa ggcactgttg
     5701 caaaaatgtg gaggtgatag gggtactacg ccggaacccc agatttttcc gtccgttcaa
     5761 attatccacg gttgaacagc ttgatcccgt ccgcccgcag ttgaccaaca ggggaaatat
     5821 gcctgtacaa agtttgccgg gttatcccaa gttcctggca tagcgtactg acctttgttt
     5881 ctgattgtcc cattgatgcc attgccagtc gcagtttgac tggcgtcatt ttataaggtc
     5941 ggccgccgtt ccgcccacgg gctcttgctg atgccagacc tgcagtcgtt ctttcagcaa
     6001 tcagttcgcg ctcaaactcc gccagtgcgg caaagatacc gaagacaagc ttgccagcgg
     6061 ccgttgttgt gtcgatagtc gccccgtgac cggtcagcac tttcagaccg gtccccctcg
     6121 cggtcaggtc gtgcactgta ttaatgagat gacgaagatc acgaccgagg cggtccagct
     6181 tccatacgac cagcgtgtct ccgggtcgta gtgctttcag acaggctgta agtccaggac
     6241 gctcttctcg tttgcccgat gcccggtcct cataaaaatg tgcggcatca acacctgcgg
     6301 cgatcagtgc atcacgttgt aaatcattcg tctgcgaacc atcagctttt gacatccgaa
     6361 tatatccgat gaacatattt cctctgtcac atatacgttc gattatgtga cattctgatt
     6421 tgggtaccat aatctgtcaa cggtagtcac attacccgta atttaaatta tgacacgtaa
     6481 aggttctatg ttaatgataa tgtgacaagc gcgtttaaat aggcataagg atcagagatg
     6541 cctactaaaa ataagctcct cagtattctt tcggacgccg agcaggaagc gctctatggt
     6601 ctacctgatt tcgatgatgc acagcggctg gagtttctgg cgctgaatgg atatgaactg
     6661 gcgctggcct gcagtcgccg ggggcttcac gctcaaattt attgcatcat ccagatcgga
     6721 gagcatgaaa aacctcgggg cagccatcga atgatgccat ctacaggggt taaaataacc
     6781 accatcattt cataacaata cctgctgact atgaagatct acgtcatcaa aatagcagtt
     6841 catggagtca gtccgatggt ctggcgtcgg ctgagaattg ctgccgacac gtcactggcc
     6901 gcgcttcact tcattttcca gatagtgcag ggctgggggg acgaccatct ccatcaattt
     6961 cacatttatg gcaaagatta cggtatctcc tacgaaggag gcatcggttt tgtcgataat
     7021 ccgttccggg tcgtgattga tgattttgct tttgatgctg gcgatcgctt tacctatgaa
     7081 tacaatttct ttgagcactg gcttcatgac attcgtgttg aggccattta tgaaaactct
     7141 acgctgaaag cgccgttttg tataagcggc catggtatgc ccggagccac agctgcggat
     7201 gagttcgata aaaccctggc gtttcttgaa gctatcgtta atgcggatga tgaaacaacg
     7261 gtcggcgaga tccgcccttt tgctgacgac ctggatgctg tcaggttcaa ccgccacaaa
     7321 atcaaccggc aactgagcag gctcgacctt gcatctccgg tactggagcc tgaagttatc
     7381 tggctgggcc gtcgtcgctg actgatgtct ataaaggtat acgttttcag catgcctctc
     7441 agatccccga aaaaatgtct ataaacctcc ctaaatcagt gtttatggat tattctttaa
     7501 atctctatga tgtttaaaga ccttccggag gttttgcgtg caatatgcct acatcagggt
     7561 aagctcggcc gatcagaata cggcccgtca ggaagaggca ttgtcaaagg cgggttttca
     7621 gcctgataaa atttgtgttg agcatgccag cgcgaaggac acaaaccgcc cggggttaca
     7681 ggaactcctg gggcaattac gccctggtga tacgcttctg gttcattcca tcgatcgcct
     7741 ttgccggaat atgtcggata tgtgcgctgt caccacccgg cttcgtgacc agggcgttac
     7801 ccttattttt ctgaaagagc agctgacgtt cagcgccggc acaaacaatc cgatgcagga
     7861 actgcagctg cacatgatgt cggcattcag ccagtttgaa cgagccttac tgaaggaaag
     7921 gcaggccgac ggcatcgccg cgaaaaaagc acggggtgaa aaaacagggc gccccggcgc
     7981 cgatatcaaa aagatccagg aaattgacgc actcagaagc agaggggtca ggctcaggat
     8041 cgcctgcgac catgcgggcc ttggcgtttc cacatattat aagcttcgtc accagatgaa
     8101 caaccagtag ccgggagata gtcatccacc acagcaggag gcaatgatgc aactgacgct
     8161 ccagatagtc atcaccgatg aatcaggctc cagtcgaacg gaggaactga tgacaatcca
     8221 gaaatcaggg gagacccgga acgacatcgg attatcggtg tcagaatcca agctgttgct
     8281 gaatacggtc cagcagtcgg tggtccagct gcaggcagac gaatatactc aacaccatat
     8341 ccggtgccct cactgccttg ctgcgcgcag aatcaaaggc aaacagaaaa tacggtaccg
     8401 gacgctgttt ggcgttattc cggtatccgg gcttcgggtg taccggtgcc gttgtgaaga
     8461 gagtgataca aagacagtca gtttgctcag cgactgggcc ggcgattatt ctcacccggc
     8521 actgaaatat attgaaaccc gctgggcctc gatgatctcc tatgaaatga cgactcgtct
     8581 gctgaaagat attctgccgg tgggccacag cctgaatgcc tcaacggtga ggaatcattt
     8641 atgccaggtg gcgcagcgtc ttgacgctga ggctgaagct cattcaggtt ttctttccgg
     8701 ctgcccccgc gactggggga acctgcccag gccaggaaaa ccgcaattaa taacctgagc
     8761 caaagtaaca agaacctcat ctccgacccc atgcccgaag ttatcattta tttttttaaa
     8821 aaaatcaaca tcaatagcaa taactgaagc ccctgaaccc tgcttgtgat tttgtatttt
     8881 atttgaaaac cccagtctgt tgttcacgcc ggttagggga tctttaagcg ccacctcatt
     8941 tagagaatca actctttcta tcattatctt aatatgcgta aaaagtgcct tttttagtcg
     9001 gtcggcttcg acataccaag cattgatacc tttaatatca actagcgaac cccctatatc
     9061 tttagcattg gtagatatag caagcttttc taaaggaatt gaaatacggg aagcaatgaa
     9121 atatgaaaaa agtgaaaatg taataactat tacaattagc agaacaagta cactcttagc
     9181 ataagtaata agaatagtag tcacgctgtc agcgtgacta taaaccaaca cactccaagc
     9241 agtatttttc atatgagcat aaccaagaag atatttattc cccgaagaaa aaaagatacc
     9301 ctcacctctc tcagacgaag ttaacttcga tttcaaatta tcatccataa ccattggtcg
     9361 accaacagca ctgttatcgt tattaaagat cacatttcca tcatcactaa caatgctaat
     9421 ttcagtgtcg cttttgaaaa aatgcctgct caaaacgtcg ctaaataaac tctgttcctt
     9481 tagatatata gacccgccga cataccctat ataattacta ttcttatcat aaataggatg
     9541 tgacaaaaaa ataatcaaat tcccagaagc tgatttgtaa ggtgccgata taaatggttt
     9601 tttagcctca atcgccaact tactggcact tgaattcaag tgaactccaa ctaaaccaag
     9661 tgattctggc gaagtcgcca gaacaacagc accatcacta acaacaagaa ctgaattaaa
     9721 cataccagac tgcaatctta acctatccgc ctcattttta agtgagtcaa tatcattagt
     9781 tgactctatt ttttttgcgc catacgccaa ttcacgttga gcaatttcaa tataattact
     9841 cattacatct gacattttca tagcgtacga tgaattagca tcaagcaagt ctttttctat
     9901 attttcgcgt ttaaaaacca taataacgac aatcagaaaa atagaagtca acaaaatacc
     9961 accaacggac aaggttatga ctatcggacg aaggtgaggc tttttaattc tcataaaaat
    10021 acactctata taattaaaaa attccac
//
