LOCUS       NC_019110              10047 bp    DNA     circular CON 27-JAN-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Typhimurium plasmid
            pHLR25, complete sequence.
ACCESSION   NC_019110
VERSION     NC_019110.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14229364
KEYWORDS    RefSeq; complete replicon.
SOURCE      Salmonella enterica subsp. enterica serovar Typhimurium (Salmonella
            typhimurium)
  ORGANISM  Salmonella enterica subsp. enterica serovar Typhimurium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1
  AUTHORS   Campos,M.J., Palomo,G., Hormeno,L., Herrera-Leon,S., Dominguez,L.,
            Vadillo,S., Piriz,S. and Quesada,A.
  TITLE     Prevalence of quinolone resistance determinants in non-typhoidal
            Salmonella isolates from human origin in Extremadura, Spain
  JOURNAL   Diagn Microbiol Infect Dis 79 (1), 64-69 (2014)
   PUBMED   24581744
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Quesada,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2012) Quesada A., University of Extremadura,
            Biochemistry, Av. de la Universidad s/n, Caceres E-10071, SPAIN
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/27/2024 14:12:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 14
            CDSs (total)                      :: 14
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            HE652087.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Salmonella enterica subsp. enterica serovar
                     Typhimurium"
                     /mol_type="genomic DNA"
                     /serovar="Typhimurium"
                     /isolate="HLR25"
                     /isolation_source="human clinical isolate"
                     /sub_species="enterica"
                     /db_xref="taxon:90371"
                     /plasmid="pHLR25"
                     /geo_loc_name="Spain:Extremadura, Llerena"
     gene            join(9450..10047,1..>8)
                     /locus_tag="HXG25_RS00005"
                     /pseudo=""
     CDS             join(9450..10047,1..>8)
                     /locus_tag="HXG25_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /name="ISKra4-like element ISKpn19 family transposase"
     gene            complement(join(9983..10047,1..1312))
                     /locus_tag="HXG25_RS00010"
     CDS             complement(join(9983..10047,1..1312))
                     /locus_tag="HXG25_RS00010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0009975 - cyclase activity [Evidence IEA]"
                     /GO_process="GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="WP_080196049.1"
                     /translation="MVNVEFFNYIECIFMRIKKPHLRPIVITLSVGGILLTSIFLIVVI
                     MVFKRENIEKDLLDANSSYAMKMSDVMSNYIEIAQRELAYGAKKIESTNDIDSLKNEAD
                     RLRLQSGMFNSVLVVSDGAVVLATSPESLGLVGVHLNSSASKLAIEAKKPFISAPYKSA
                     SGNLIIFLSHPIYDKNSNYIGYVGGSIYLKEQSLFSDVLSRHFFKSDTEISIVSDDGNV
                     IFNNDNSAVGRPMVMDDNLKSKLTSSERGEGIFFSSGNKYLLGYAHMKNTAWSVLVYSH
                     ADSVTTILITYAKSVLVLLIVIVITFSLFSYFIASRISIPLEKLAISTNAKDIGGSLVD
                     IKGINAWYVEADRLKKALFTHIKIMIERVDSLNEVALKDPLTGVNNRLGFSNKIQNHKQ
                     GSGASVIAIDVDFFKKINDNFGHGVGDEVLVTLAQVINCGFPGLGRFPQSRGQPERKPE
                     "
                     /name="diguanylate cyclase"
     gene            complement(1618..1833)
                     /locus_tag="HXG25_RS00015"
     CDS             complement(1618..1833)
                     /locus_tag="HXG25_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137421.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1837..2322)
                     /locus_tag="HXG25_RS00020"
                     /old_locus_tag="D645_p6002"
     CDS             complement(1837..2322)
                     /locus_tag="HXG25_RS00020"
                     /old_locus_tag="D645_p6002"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012482081.1"
                     /translation="MSSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEQSVRAELNESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSVLLIASSAGILWWQSQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRSCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2357..3061)
                     /locus_tag="HXG25_RS00080"
                     /pseudo=""
     CDS             complement(<2357..3061)
                     /locus_tag="HXG25_RS00080"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3000..3323)
                     /locus_tag="HXG25_RS00030"
     CDS             complement(3000..3323)
                     /locus_tag="HXG25_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_015059464.1"
                     /translation="MLTIWVTEDEHRRLLDRCDGKQLAAWMRQTCLDEKPARAAKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3973..4131
                     /locus_tag="HXG25_RS00035"
     CDS             3973..4131
                     /locus_tag="HXG25_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001658417.1"
                     /translation="MHEPPVQSDCCASSGNFQLDSNPERHDKAPLAAAIGNWELVDLDI
                     ESLEVVA"
                     /name="hypothetical protein"
     gene            complement(4374..4679)
                     /locus_tag="HXG25_RS00040"
     CDS             complement(4374..4679)
                     /locus_tag="HXG25_RS00040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA];
                     GO:0046872 - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="WP_223229636.1"
                     /translation="MPKPDNSCCRSEDIVCRFGGEEFVVFLPNTSVVTAECVAERIRSV
                     IESTVFPNNLRVTVSAGVATQEDPLGGIDFLLKNADDALYQAKGEGRNKVIVYSAI"
                     /name="GGDEF domain-containing protein"
     gene            complement(<4665..5695)
                     /locus_tag="HXG25_RS00045"
                     /pseudo=""
     CDS             complement(<4665..5695)
                     /locus_tag="HXG25_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612174.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            6090..6746
                     /locus_tag="HXG25_RS00050"
                     /old_locus_tag="D645_p6003"
     CDS             6090..6746
                     /locus_tag="HXG25_RS00050"
                     /old_locus_tag="D645_p6003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="WP_001516695.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFVN
                     CKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRTNF
                     AHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLSRGV
                     FSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrS1"
     gene            complement(7065..7679)
                     /locus_tag="HXG25_RS00055"
     CDS             complement(7065..7679)
                     /locus_tag="HXG25_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412822.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_014839983.1"
                     /translation="MFIGYIRMSKADGSQTNDLQRDALIAAGVDAAHFYEDRASGKREE
                     RPGLTACLKALRPGDTLVVWKLDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATIDTT
                     TAAGKLVFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMASMG
                     QSETKVSTLCQELGITRQTLYRHISPVGQLRADGIKLFNRG"
                     /name="recombinase family protein"
     gene            7841..>8023
                     /locus_tag="HXG25_RS00060"
                     /pseudo=""
     CDS             7841..>8023
                     /locus_tag="HXG25_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     gene            8159..8704
                     /locus_tag="HXG25_RS00065"
     CDS             8159..8704
                     /locus_tag="HXG25_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="WP_001493763.1"
                     /translation="MVWRRLRIAADTSLAALHFIFQIVQGWGDDHLHQFHIYGKDYGIS
                     YEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIRVEAIYENSTLKAPFCIS
                     GHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADDLDAVRFNRHKINRQLSRL
                     DLASPVLEPEVIWLGRRR"
                     /name="plasmid pRiA4b ORF-3 family protein"
     gene            8841..9413
                     /locus_tag="HXG25_RS00070"
     CDS             8841..9413
                     /locus_tag="HXG25_RS00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001493762.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNRP
                     GLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGTNN
                     PMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALRSRG
                     VRLRIACDHAGLGVSTYYKLRHQMNNQ"
                     /name="recombinase family protein"
     ARG             6091..6743
                     /name="qnrS1"
                     /gene="qnrS1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrS1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          3594..4140
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.652"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(1515..3010)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.583"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aattaataac ctgagccaaa gtaacaagaa cctcatctcc gaccccatgc ccgaagttat
       61 catttatttt tttaaaaaaa tcaacatcaa tagcaataac tgaagcccct gaaccctgct
      121 tgtgattttg tattttattt gaaaacccca gtctgttgtt cacgccggtt aggggatctt
      181 taagcgccac ctcatttaga gaatcaactc tttctatcat tatcttaata tgcgtaaaaa
      241 gtgccttttt tagtcggtcg gcttcgacat accaagcatt gataccttta atatcaacta
      301 gcgaaccccc tatatcttta gcattggtag atatagcaag cttttctaaa ggaattgaaa
      361 tacgggaagc aatgaaatat gaaaaaagtg aaaatgtaat aactattaca attagcagaa
      421 caagtacact cttagcataa gtaataagaa tagtagtcac gctgtcagcg tgactataaa
      481 ccaacacact ccaagcagta tttttcatat gagcataacc aagaagatat ttattccccg
      541 aagaaaaaaa gataccctca cctctctcag acgaagttaa cttcgatttc aaattatcat
      601 ccataaccat tggtcgacca acagcactgt tatcgttatt aaagatcaca tttccatcat
      661 cactaacaat gctaatttca gtgtcgcttt tgaaaaaatg cctgctcaaa acgtcgctaa
      721 ataaactctg ttcctttaga tatatagacc cgccgacata ccctatataa ttactattct
      781 tatcataaat aggatgtgac aaaaaaataa tcaaattccc agaagctgat ttgtaaggtg
      841 ccgatataaa tggtttttta gcctcaatcg ccaacttact ggcacttgaa ttcaagtgaa
      901 ctccaactaa accaagtgat tctggcgaag tcgccagaac aacagcacca tcactaacaa
      961 caagaactga attaaacata ccagactgca atcttaacct atccgcctca tttttaagtg
     1021 agtcaatatc attagttgac tctatttttt ttgcgccata cgccaattca cgttgagcaa
     1081 tttcaatata attactcatt acatctgaca ttttcatagc gtacgatgaa ttagcatcaa
     1141 gcaagtcttt ttctatattt tcgcgtttaa aaaccataat aacgacaatc agaaaaatag
     1201 aagtcaacaa aataccacca acggacaagg ttatgactat cggacgaagg tgaggctttt
     1261 taattctcat aaaaatacac tctatataat taaaaaattc cacatttacc ataacatccc
     1321 ttgcccgcac cgccccggac gggttcggcg cgaatgcggc gtcagcccgg taaaaccggc
     1381 agtaaactcc ccgctaaacc gcgctttttg agccagataa atttccccaa gatgcatagc
     1441 catgcagccg gggggatggc accgcctttc ggcggggatc tgcgccgaaa gacccgtttt
     1501 atccggtttg ttacagcgac ggaccgttgt acgtccgctg gcgctgcgat cgttccagct
     1561 ccagcgcttt ccggtgctgc gccgcccgtt cctgctcccg gtgcgcctcg ataccattca
     1621 gccttgcgac aaccggcgca gctgctcgct caaactctgc acctgcctgc tcaagcccgt
     1681 gacgcgctcg ctcagcgccg cgttctcccg ttgcataaga ccagacatcg tccgccattc
     1741 cgcgaaggcg ttctcccact cgtccagcct tttcgagtag tcctgctgta gctgctctaa
     1801 tgcgctcagc aactgttttt ccagctccgt catgtgctat ttccccgcca gtatcatcca
     1861 gctcgaatcc tctccgaacc gtcccgcttc cgggttcacc ctcacgcagc tgcgtctctg
     1921 ctcgccgcag gtcgagagct gcacgccgct gttccgctcg gacagcatcg cctgcgtgct
     1981 cttctgctcc cggatggtcg tgtaattgtc gagtatctgc tgactctgcc accacagaat
     2041 gcccgcgctc gatgcgatca gcaggacgga aaccaggacg atggtcagcc acgtctggct
     2101 gaccatacgc accatgcctt tcgtgcgctg gctcatggct gaggacagct tccggtcgtg
     2161 gtcgaggatg gcggcgctga ttctcttctc gctttcgttc agttccgctc tgacagactg
     2221 ctcgtgctcg ctgaatgcgg ctttcagcat ctcgccggta ctctgctgct gcgctttcga
     2281 tttctgctct aagtccttcg ccagcgttaa aagactgctc atagatggct ccctttagtc
     2341 ggatgtttcg ccccccgtcc gggtcggcaa tgctgatact gcttttcgtg gtgcgtacca
     2401 cctcaaaacc cgcgttttcc agcgcctcag taacgtcctg acgggttttc agctccccgg
     2461 acgaggctaa ggccagcaaa ccccgggtga tcgcctgagc tgcctcctgc ttcgtttccg
     2521 gcagcgcgga cggcgtgacc agcgcccgcc ggttttccgg cgcattcggg tcgtgcagcc
     2581 ccagcctgcc gttcacgatg gtctgccagg catcgatgcg cggacggtca gcgcggtcgt
     2641 aatacggctg gagccttttg ccggtcagca gctccgtgtt cgggatcagg aagttcagct
     2701 ccagccgccc cttgtcctgg tgctccaccc acagcacgct gtactggtct ttatcgagtc
     2761 cgggcatcag aacacgctcg aagctcgcca tcagtttttc acgctgaccg ggcggtaagt
     2821 cctgttcggc aaacgacagc accccggagg tgtacttttt ggcgtagggc gaggcgtcga
     2881 ttagctcccg gacttcctcc ggtttaccct gcagaacgct ggcgccgtcg cgcttgcggt
     2941 cattacccag cagataatcc accggaccgg caccgccgcc gcgccctcgt gggtgaaatt
     3001 taacgatcat cgtcagcccc cttttccagc acagcgtgcc gcaggcgctc aagtccggca
     3061 tcaatggcca tcagcgcagc gacaatctgt acgcgatcat gtccggtacc accaccggcg
     3121 ttaacctgcc gggcaatctg attgaggttg tttcccatac cggcaagttg acgaagcaac
     3181 gccggcgaga tcgacggaag tttggcggca cgagcaggtt tctcgtcgag acacgtctgc
     3241 cgcatccagg ccgcgagctg cttaccgtcg cagcgatcca gcagacgccg gtgctcgtct
     3301 tcagttaccc atatcgtgag cattttgctg cgtttgtctg ccaatccggc ctcctgataa
     3361 cgccgggacg gacggggaat tatttcccgg tcggcgcggt gttggacaag ccgcaggcgc
     3421 gtcagtgcct tttagcgggt ttcggggcgc agccctgaac cagtcacgta gcgctagcgg
     3481 agtgtatact ggcttaaaat gtaacatcgc agaaacatca agcatagctg ccacataaag
     3541 caggtgaaaa atgagattcg tatgcgtcag cagaagatgc actgccggag aaatgccgct
     3601 tcctcgctca ctgactcgct ccgctcggtc gttcggctgc ggcgagcggt accggcttac
     3661 gggcggggcg gaaatttcct ggaagatgct aggaagaaac ttaacaggga agcgataagg
     3721 ccacggcaga gccgtttttc cataggctcc gcccccctga caagcatcac agaatctgac
     3781 gctcaaatca gtggtggcga aacccgacag gactataaag atcccaggcg tttccccctg
     3841 gtagctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     3901 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     3961 aagctggact gtatgcacga accccccgtt cagtccgact gctgcgcctc ctccggtaac
     4021 tttcaacttg attccaaccc ggaaaggcac gacaaagcgc cactggcagc agccattggt
     4081 aactgggaat tagtggattt agatatcgag agtcttgaag tggtggcctg actacggcta
     4141 cactgaaagg acagtatttg gtatctgcgc tccactaaag ccagttacct tcggaagaaa
     4201 gtgttggtag ctcttaattc cggcaaacga accaccgttg gtagcggtgg tttttttgtt
     4261 tacagggcat gagcttacga cgacatcgaa gggataacaa gaggatccgc ttcttgaacc
     4321 cttttcccca caaaaaaacg ccacactagc gcgtaaagcg tcatcaaaaa aactcaaata
     4381 gcgctatata caatcacttt attacgcccc tctcccttgg cttgataaag agcatcatca
     4441 gcatttttca gcaaaaaatc gatcccacca agaggatcct cttgtgttgc cacaccagca
     4501 gagacggtga cccgcaagtt attagggaaa acagtacttt caataacact cctaatacgc
     4561 tccgcaacac actccgctgt taccacagat gtattcggca gaaaaacaac aaactcttct
     4621 ccaccaaacc gacaaacaat atcttcacta cggcagcaag aattatccgg tttcggcatg
     4681 atgctgatgt agtcccgttt tatcgtcatc acgaagcttt ctgctatgcc attgctttcc
     4741 gggcttcgca ctgctgtcgt acaaggctcc agtcccagca accgggcgaa cgccggcact
     4801 gttggcaact gccttccgaa gcgtttttcc accgcaccca gcatgacatc ctgcaccgtt
     4861 tctttgtcat agcctccggt gctcgctgcc caatcaatga tctccctgtc gcagcagtcc
     4921 tgcgcgaagg tcacccgcag cttttcgcca ttatcacagc ggaactcaaa gccatctgag
     4981 caccagcgcc ggttactttc cgctactgct acgcgcccct tatgggcccg cttacggcaa
     5041 ggatcagcgg gtttacgttc aagaagcaga ttatgcgttc tcataatgcg atacacccgc
     5101 tttgcattaa ccacaggcag gccgtcccgc tccgactccc gtcgcagcag cgcccacaca
     5161 cggcgataac catacgtcgg caggtcagcc accgccatgt ttatccggga caatacggtg
     5221 gtatcgtcag aacggggctg ccgtctgcga tcctgtcagt cagatggccg gtgaacccga
     5281 atgctcaact gcgcacgcga cacgccaaga cttctgcaaa cgtcgcttat tcgtcgttcc
     5341 caggcaacga gggcgcatgc gcaatccatt tttttgcccg gccgaactcc acggcctctt
     5401 tgagtatctc ggcttccatc gtctttttgc ccagaaggcg ctggagttcg cgaatttgct
     5461 tattggcggc agcgagttcg gaggccggca ccacttcctc gcccgatgca acagccgtca
     5521 gtgaaccgtc ttaatattgc ttgcgccatt tgaatatctg gtttgcattg atgccatgca
     5581 ggcgcgcgac atgagacacg gtcataccgg gctccatagt ctgctgaata atggcaattt
     5641 tttcctgcgg tgtacgacgc cgacgccgct caggtcctga taacacttca accatcttat
     5701 cgttctgact ggtattaaac atagttccaa gactacctct tattttaaga gagtcgaagt
     5761 gtctggtgat ctatggggcc agtctagccc tcttccaagt gtagggagat atagtcaaag
     5821 tttgcttctc cgccgatgag tccgccctgc gtcggatagt ccgatttttt accggcgaag
     5881 cacggctctc ctaaccctat ctaaagcgaa taactttttt caacagggcc gcgtgccaat
     5941 gcaagagcga taggcgctct acgcgccaat ttgaccactt aaaacaggta aattgagggg
     6001 ttgtaatgtg ttgatgtaac aggcttttat tttaatgtct tggcatatgt ataatggtag
     6061 tctagccctc ctttcaacaa ggagtactca tggaaaccta caatcataca tatcggcacc
     6121 acaacttttc acataaagac ttaagtgatc tcaccttcac cgcttgcaca ttcattcgca
     6181 gcgactttcg acgtgctaac ttgcgtgata cgacattcgt caactgcaag ttcattgaac
     6241 agggtgatat cgaaggctgc cactttgatg tcgcagatct tcgtgatgca agtttccaac
     6301 aatgccaact tgcgatggca aacttcagta atgccaattg ctacggtata gagttccgtg
     6361 cgtgtgattt aaaaggtgcc aacttttccc gaacaaactt tgcccatcaa gtgagtaatc
     6421 gtatgtactt ttgctcagca tttatttctg gatgtaatct ttcctatgcc aatatggaga
     6481 gggtttgttt agaaaaatgt gagttgtttg aaaatcgctg gataggaacg aacctagcgg
     6541 gtgcatcact gaaagagtca gacttaagtc gaggtgtttt ttccgaagat gtctgggggc
     6601 aatttagcct acagggtgcc aatttatgcc acgccgaact cgacggttta gatccccgca
     6661 aagtcgatac atcaggtatc aaaattgcag cctggcagca agaactgatt ctcgaagcac
     6721 tgggtattgt tgtttatcct gactaattgc tttgatgtgt gattttaaac gctcaaattt
     6781 ataaaacgaa taatattgtc aaccttgcaa taatagacat agggacaacc atgtataaat
     6841 ttttaccctc aggcgtcttc acaatagagc catatagcag tgggtcactt agtgggctta
     6901 atttttccat taaagataat attaatattg ctcagtataa aacatcatat ggtagcccat
     6961 cttggcaagt aaacataagg ctgccattta taaggcactg ttgcaaaaat gtggaggtga
     7021 taggggtact acgccggaac cccagatttt tccgtccgtt caaattatcc acggttgaac
     7081 agcttgatcc cgtccgcccg cagttgacca acaggggaaa tatgcctgta caaagtttgc
     7141 cgggttatcc caagttcctg gcatagcgta ctgacctttg tttctgattg tcccattgat
     7201 gccattgcca gtcgcagttt gactggcgtc attttataag gtcggccgcc gttccgccca
     7261 cgggctcttg ctgatgccag acctgcagtc gttctttcag caatcagttc gcgctcaaac
     7321 tccgccagtg cggcaaagat accgaagaca agcttgccag cggccgttgt tgtgtcgata
     7381 gtcgccccgt gaccggtcag cactttcaga ccggtccccc tcgcggtcag gtcgtgcact
     7441 gtattaatga gatgacgaag atcacgaccg aggcggtcca gcttccatac gaccagcgtg
     7501 tctccgggtc gtagtgcttt cagacaggct gtaagtccag gacgctcttc tcgtttgccc
     7561 gatgcccggt cctcataaaa atgtgcggca tcaacacctg cggcgatcag tgcatcacgt
     7621 tgtaaatcat tcgtctgcga accatcagct tttgacatcc gaatatatcc gatgaacata
     7681 tttcctctgt cacatatacg ttcgattatg tgacattctg atttgggtac cataatctgt
     7741 caacggtagt cacattaccc gtaatttaaa ttatgacacg taaaggttct atgttaatga
     7801 taatgtgaca agcgcgttta aataggcata aggatcagag atgcctacta aaaataagct
     7861 cctcagtatt ctttcggacg ccgagcagga agcgctctat ggtctacctg atttcgatga
     7921 tgcacagcgg ctggagtttc tggcgctgaa tggatatgaa ctggcgctgg cctgcagtcg
     7981 ccgggggctt cacgctcaaa tttattgcat catccagatc ggagagcatg aaaaacctcg
     8041 gggcagccat cgaatgatgc catctacagg ggttaaaata accaccatca tttcataaca
     8101 atacctgctg actatgaaga tctacgtcat caaaatagca gttcatggag tcagtccgat
     8161 ggtctggcgt cggctgagaa ttgctgccga cacgtcactg gccgcgcttc acttcatttt
     8221 ccagatagtg cagggctggg gggacgacca tctccatcaa tttcacattt atggcaaaga
     8281 ttacggtatc tcctacgaag gaggcatcgg ttttgtcgat aatccgttcc gggtcgtgat
     8341 tgatgatttt gcttttgatg ctggcgatcg ctttacctat gaatacaatt tctttgagca
     8401 ctggcttcat gacattcgtg ttgaggccat ttatgaaaac tctacgctga aagcgccgtt
     8461 ttgtataagc ggccatggta tgcccggagc cacagctgcg gatgagttcg ataaaaccct
     8521 ggcgtttctt gaagctatcg ttaatgcgga tgatgaaaca acggtcggcg agatccgccc
     8581 ttttgctgac gacctggatg ctgtcaggtt caaccgccac aaaatcaacc ggcaactgag
     8641 caggctcgac cttgcatctc cggtactgga gcctgaagtt atctggctgg gccgtcgtcg
     8701 ctgactgatg tctataaagg tatacgtttt cagcatgcct ctcagatccc cgaaaaaatg
     8761 tctataaacc tccctaaatc agtgtttatg gattattctt taaatctcta tgatgtttaa
     8821 agaccttccg gaggttttgc gtgcaatatg cctacatcag ggtaagctcg gccgatcaga
     8881 atacggcccg tcaggaagag gcattgtcaa aggcgggttt tcagcctgat aaaatttgtg
     8941 ttgagcatgc cagcgcgaag gacacaaacc gcccggggtt acaggaactc ctggggcaat
     9001 tacgccctgg tgatacgctt ctggttcatt ccatcgatcg cctttgccgg aatatgtcgg
     9061 atatgtgcgc tgtcaccacc cggcttcgtg accagggcgt tacccttatt tttctgaaag
     9121 agcagctgac gttcagcgcc ggcacaaaca atccgatgca ggaactgcag ctgcacatga
     9181 tgtcggcatt cagccagttt gaacgagcct tactgaagga aaggcaggcc gacggcatcg
     9241 ccgcgaaaaa agcacggggt gaaaaaacag ggcgccccgg cgccgatatc aaaaagatcc
     9301 aggaaattga cgcactcaga agcagagggg tcaggctcag gatcgcctgc gaccatgcgg
     9361 gccttggcgt ttccacatat tataagcttc gtcaccagat gaacaaccag tagccgggag
     9421 atagtcatcc accacagcag gaggcaatga tgcaactgac gctccagata gtcatcaccg
     9481 atgaatcagg ctccagtcga acggaggaac tgatgacaat ccagaaatca ggggagaccc
     9541 ggaacgacat cggattatcg gtgtcagaat ccaagctgtt gctgaatacg gtccagcagt
     9601 cggtggtcca gctgcaggca gacgaatata ctcaacacca tatccggtgc cctcactgcc
     9661 ttgctgcgcg cagaatcaaa ggcaaacaga aaatacggta ccggacgctg tttggcgtta
     9721 ttccggtatc cgggcttcgg gtgtaccggt gccgttgtga agagagtgat acaaagacag
     9781 tcagtttgct cagcgactgg gccggcgatt attctcaccc ggcactgaaa tatattgaaa
     9841 cccgctgggc ctcgatgatc tcctatgaaa tgacgactcg tctgctgaaa gatattctgc
     9901 cggtgggcca cagcctgaat gcctcaacgg tgaggaatca tttatgccag gtggcgcagc
     9961 gtcttgacgc tgaggctgaa gctcattcag gttttctttc cggctgcccc cgcgactggg
    10021 ggaacctgcc caggccagga aaaccgc
//
