LOCUS       NC_019085               2989 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli plasmid pPAB19-3, complete sequence.
ACCESSION   NC_019085
VERSION     NC_019085.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226150
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Tran,T., Andres,P., Petroni,A., Soler-Bistue,A., Albornoz,E.,
            Zorreguieta,A., Reyes-Lamothe,R., Sherratt,D.J., Corso,A. and
            Tolmasky,M.E.
  TITLE     Small plasmids harboring qnrB19: a model for plasmid evolution
            mediated by site-specific recombination at oriT and Xer sites
  JOURNAL   Antimicrob Agents Chemother 56 (4), 1821-1827 (2012)
   PUBMED   22290975
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Andres,P., Soler Bistue,A., Lucero,C., Tolmasky,M.E., Corso,A. and
            Petroni,A.
  TITLE     Epidemiology of plasmid mediated quinolone resistance mechanisms in
            Argentina: differential distribution in enterobacteria
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 2989)
  AUTHORS   Andres,P., Soler Bistue,A., Zorreguieta,A., Corso,A., Tolmasky,M.E.
            and Petroni,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2011) Servicio Antimicrobianos, Instituto Nacional
            de Enfermedades Infecciosas, ANLIS 'Dr. Carlos G. Malbran', Av.
            Velez Sarsfield 563, Buenos Aires C1282AFF, Argentina
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009958225.2-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 03:13:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3
            CDSs (total)                      :: 3
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JN985534.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="M9888"
                     /db_xref="taxon:562"
                     /plasmid="pPAB19-3"
                     /geo_loc_name="Argentina"
                     /note="clinical isolate; qnrB19-harboring small ColE1-type
                     plasmid"
     gene            886..1035
                     /locus_tag="HS838_RS00005"
     CDS             886..1035
                     /locus_tag="HS838_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162846302.1"
                     /translation="MPSEYDEQRGSTDTQLPGEYDLFDLMAKVTEEILHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(2044..2688)
                     /locus_tag="HS838_RS00010"
                     /old_locus_tag="D616_p74002"
     CDS             complement(2044..2688)
                     /locus_tag="HS838_RS00010"
                     /old_locus_tag="D616_p74002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(2782..>2937)
                     /locus_tag="HS838_RS00015"
                     /old_locus_tag="D616_p74001"
                     /pseudo=""
     CDS             complement(2782..>2937)
                     /locus_tag="HS838_RS00015"
                     /old_locus_tag="D616_p74001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     ARG             complement(2048..2688)
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          678..851
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(208..699)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 gatcttcttg agatcctttc tgtggcgtcg taatctcttg ctctgtaaac gaaaaaaccg
       61 ccctgcaagg cggtttttcg aaggttctca gagcagcaac tctttgaacc gtggtaactg
      121 gcttacgagg agcgcagtaa ccaaatctgt tctttcagtt tagccttaac aggcgcataa
      181 cttcaagact aactcctcta aatcagttac cagtggctgc tgccagtggc gataagtcgt
      241 gtctttccgg gttggactca agatgatagt taccggataa ggcgcagcag tcggactgaa
      301 cggggggttc gtgcatacag tccatccagg agcgaactgc cttcgcggaa ctgagtgtca
      361 ggcgtggaat gagataaacg cggccataac agcggaatga caccggtaaa ccgaaaggca
      421 ggaatgcggg ggagcacgag ggagccacca gggggaaacg cctggtatct ttaagccgca
      481 tcgggtttcg ccaccactga tttgagcgtc agatttcatg atgtttgtca ggggggcgga
      541 gcctatggaa aaacggcttc gccgcggcct tatctcttcc ctgttaagct ccttcctggt
      601 atctcccagg aaatttccgc cccgcctgta agccggcacc gctcgccgca gccgaacgac
      661 cgagcgcagc gagtcagtga gcgaggaagc ggaatatatc ctgtactaca tatcctgctg
      721 acgcgctggt gccgcctttt ttctcctgct acatgaagca cttcactgat ttccacatcc
      781 gtgccaacat agtcagccag tatacactcc gctagcgcta cgtgactggt tcagggctgc
      841 gccccgaaac ccgctaacaa cctctctgct actggatatc accccatgcc atctgaatat
      901 gatgaacaac ggggttcgac tgatacgcag cttcccggcg aatatgacct ttttgattta
      961 atggcgaagg ttactgaaga gatcctgcat gcccctgtgg attctgggcc gcctgtcggc
     1021 cgggaagcgt tatagctttt tcgcttgccc gcgtacgtgg taatacgccg gtattacctt
     1081 ttatttcttt attttcatgt aattacagtg ggtttttaat acctgggtat tactcgtgaa
     1141 aacgaaacat tacttttttt tcttttaatt catgcagttg cgatgaaaat cgcggctgcg
     1201 ttaggtgtat gaccattttg aggttgaaaa ataatcaacc atggcgggtg atttacgcag
     1261 gttaagggga gatcgtgaca aaaaaaagcg ttcctcactg tatcgttcat gcctgcatga
     1321 acgggttatt cttcctgact acagaattac atcgctcctt cagttcatac atcactttta
     1381 caacactttt acaacactcc ttcggcctgt acatcacttt tacaacactt ttgcatcact
     1441 tttacatcat ttatacatca cttttacatc attccttcag ctcatacgtc actttcatcc
     1501 tctgtgccat ttgtacatca cccccttcct gaacgtgctg acagcgggtt ttccggctgc
     1561 gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc gttcaccgtt ttcggatggg
     1621 aagaacgccg gaaacaggac ttcccctggc tgaatggtgt gaatttccgc gctatgcact
     1681 tgcgcgcata ctcatgcatg ccgtaaaaac agagcctgcg cgtttctggc gggttttcgg
     1741 gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg ccctcagagc ctctcaggcg
     1801 gtgcacgcaa gggatgttat gtttaatcgg tctgaccgtc aggtacaaaa aaaagccctt
     1861 agggggcttt ttttttgcta tgagttagcc atattttcgt gcgaattttt catcggaatc
     1921 gcatagataa ataataaact caataaacgc gatgatcgcg ggtatgaatg tccagcaaaa
     1981 aattagatac aggaacccct taaaagtaaa tcgtcaaacg catctcccgg tgtaaacgct
     2041 gaactaacca atcacagcga tgccaagccg ctccatgagc aacgatgcct ggtagctgtc
     2101 taacttaacg ccttgtaaat caacaccccg aatatctaag tcacctaact ccgaattggt
     2161 cagatcgcaa tgtgtgaagt ttgctgctcg ccagtcgaaa gtcgaaaact cgccgccgga
     2221 gagatctgaa ccactgaacg tcgcacccag tacctgagtc cccatccagc ggttttccca
     2281 cagctcacac ttttccaaca cgactttcga aaaattggcg tagcttagat tagtattagt
     2341 gatatatgcg ctgcaaaacc aggtgcgcgt ggtgatcata ttcataaagc ttgcaccgcg
     2401 gaaatctgcg ccttgtgcgc ggcagtggcg aatttcaatg cccaaggcac tgacgttgcg
     2461 gaaatctgcc attgataaat cacagctttt gaaaatggca tctttcagca ttgcgcgact
     2521 aaaattgcac cctttctgac tttcgcgatc atagaactgg cagccgataa attcagtgcc
     2581 gctcaggtcg gcacctgaaa aatcgcagtt aaaaaatgta ctattttcaa ctttctcacc
     2641 ggtgaagcga tttctgtcaa ttttttcgcc aactaatgcc agagtcatat ctatgcctgt
     2701 gtttttatac agtaatgacg ccatggtaaa ccttatgacg ttatgcgtca aacccgccag
     2761 tatgacacct gcaaatgcgt attagatctg gtgtttcttc agcaaggcac gtagctgatg
     2821 ataagtcaga cccagtaatt cagcggcttt tttctggtta aatttagcct gctgtaagct
     2881 ggtttgtaga aagtctttct cttgctgctg ctggaattca cgcagatcca gcgttataac
     2941 gatgtgtatc ctatgtgctc ggattggata gatttccatt acggtgaag
//
