LOCUS       NC_019084               3082 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli plasmid pPAB19-2, complete sequence.
ACCESSION   NC_019084
VERSION     NC_019084.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226161
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Tran,T., Andres,P., Petroni,A., Soler-Bistue,A., Albornoz,E.,
            Zorreguieta,A., Reyes-Lamothe,R., Sherratt,D.J., Corso,A. and
            Tolmasky,M.E.
  TITLE     Small plasmids harboring qnrB19: a model for plasmid evolution
            mediated by site-specific recombination at oriT and Xer sites
  JOURNAL   Antimicrob Agents Chemother 56 (4), 1821-1827 (2012)
   PUBMED   22290975
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Andres,P., Soler Bistue,A., Lucero,C., Tolmasky,M.E., Corso,A. and
            Petroni,A.
  TITLE     Epidemiology of plasmid mediated quinolone resistance mechanisms in
            Argentina: differential distribution in enterobacteria
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3082)
  AUTHORS   Andres,P., Soler Bistue,A., Zorreguieta,A., Corso,A., Tolmasky,M.E.
            and Petroni,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2011) Servicio Antimicrobianos, Instituto Nacional
            de Enfermedades Infecciosas, ANLIS 'Dr. Carlos G. Malbran', Av.
            Velez Sarsfield 563, Buenos Aires C1282AFF, Argentina
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009958115.2-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 03:12:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3
            CDSs (total)                      :: 3
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JN979787.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="M9996"
                     /db_xref="taxon:562"
                     /plasmid="pPAB19-2"
                     /geo_loc_name="Argentina"
                     /note="clinical isolate; qnrB19-harboring small ColE1-type
                     plasmid"
     gene            886..1035
                     /locus_tag="HS868_RS00005"
     CDS             886..1035
                     /locus_tag="HS868_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162846302.1"
                     /translation="MPSEYDEQRGSTDTQLPGEYDLFDLMAKVTEEILHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(2137..2781)
                     /locus_tag="HS868_RS00010"
                     /old_locus_tag="D616_p73002"
     CDS             complement(2137..2781)
                     /locus_tag="HS868_RS00010"
                     /old_locus_tag="D616_p73002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(2875..>3030)
                     /locus_tag="HS868_RS00015"
                     /old_locus_tag="D616_p73001"
                     /pseudo=""
     CDS             complement(2875..>3030)
                     /locus_tag="HS868_RS00015"
                     /old_locus_tag="D616_p73001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     ARG             complement(2141..2781)
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          678..851
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(208..699)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 gatcttcttg agatcctttc tgtggcgtcg taatctcttg ctctgtaaac gaaaaaaccg
       61 ccctgcaagg cggtttttcg aaggttctca gagcagcaac tctttgaacc gtggtaactg
      121 gcttacgagg agcgcagtaa ccaaatctgt tctttcagtt tagccttaac aggcgcataa
      181 cttcaagact aactcctcta aatcagttac cagtggctgc tgccagtggc gataagtcgt
      241 gtctttccgg gttggactca agatgatagt taccggataa ggcgcagcag tcggactgaa
      301 cggggggttc gtgcatacag tccatccagg agcgaactgc cttcgcggaa ctgagtgtca
      361 ggcgtggaat gagataaacg cggccataac agcggaatga caccggtaaa ccgaaaggca
      421 ggaatgcggg ggagcacgag ggagccacca gggggaaacg cctggtatct ttaagccgca
      481 tcgggtttcg ccaccactga tttgagcgtc agatttcatg atgtttgtca ggggggcgga
      541 gcctatggaa aaacggcttc gccgcggcct tatctcttcc ctgttaagct ccttcctggt
      601 atctcccagg aaatttccgc cccgcctgta agccggcacc gctcgccgca gccgaacgac
      661 cgagcgcagc gagtcagtga gcgaggaagc ggaatatatc ctgtactaca tatcctgctg
      721 acgcgctggt gccgcctttt ttctcctgct acatgaagca cttcactgat ttccacatcc
      781 gtgccaacat agtcagccag tatacactcc gctagcgcta cgtgactggt tcagggctgc
      841 gccccgaaac ccgctaacaa cctctctgct actggatatc accccatgcc atctgaatat
      901 gatgaacaac ggggttcgac tgatacgcag cttcccggcg aatatgacct ttttgattta
      961 atggcgaagg ttactgaaga gatcctgcat gcccctgtgg attctgggcc gcctgtcggc
     1021 cgggaagcgt tatagctttt tcgcttgccc gcgtacgtgg taatacgccg gtattacctt
     1081 ttatttcttt attttcatgt aattacagtg ggtttttaat acctgggtat tactcgtgaa
     1141 aacgaaacat tacttttttt tcttttaatt catgcagttg cgatgaaaat cgcggctgcg
     1201 ttaggtgtat gaccattttg aggttgaaaa ataatcaacc atggcgggtg atttacgcag
     1261 gttaagggga gatcgtgaca aaaaaaagcg ttcctcactg tatcgttcat gcctgcatga
     1321 acgggttatt cttcctgact acagaattac atcgctcctt cagttcatac atcactttca
     1381 caacactttt acaacactcc ttcggcctgt acatcacttt tacaacactt ttgcatcact
     1441 tttacatcac ttttacatca tttatacatc acttttacat cattccttca gctcatacgt
     1501 cactttcatc ctctgtgcca tttgtacatc acccccttcc tgaacgtgct gacagcgggt
     1561 tttccggctg cgccggcaac gcttcacggc cacgcaataa attgcgtccc cgttcaccgt
     1621 tttcggatgg gaagaacgcc ggaaacagga cttcccctgg ctgaatggtg tgaatttccg
     1681 cgctatgcac ttgcgcgcat actcatgcat gccgtaaaaa cagagcctgc gcgtttctgg
     1741 cgggttttcg ggtggtttgt tgcctgtttt accggtttcc cgtcagaaac gccctcagag
     1801 cctctcaggc ggtgcacgca agggatgtta tgtttaatcg gtctgaccgt caggtacaaa
     1861 aaaaagccct tagggggctt tttttttgct atgagttagc catattttcg ttaagacatg
     1921 gcttcatgat tcgggagtgg ttattcgttg ttctcgcgaa aatgaattgg ttagatattc
     1981 atcaaattcc tgtccagaat gcagcattag atggcatgtc gttgatgcgg taggtcagga
     2041 tattcgtcca ctggagaaaa cgtttcacaa tgcttgccag gagtcgtact ggttaaaagt
     2101 aaatcgtcaa acgcatctcc cggtgtaaac gctgaactaa ccaatcacag cgatgccaag
     2161 ccgctccatg agcaacgatg cctggtagct gtctaactta acgccttgta aatcaacacc
     2221 ccgaatatct aagtcaccta actccgaatt ggtcagatcg caatgtgtga agtttgctgc
     2281 tcgccagtcg aaagtcgaaa actcgccgcc ggagagatct gaaccactga acgtcgcacc
     2341 cagtacctga gtccccatcc agcggttttc ccacagctca cacttttcca acacgacttt
     2401 cgaaaaattg gcgtagctta gattagtatt agtgatatat gcgctgcaaa accaggtgcg
     2461 cgtggtgatc atattcataa agcttgcacc gcggaaatct gcgccttgtg cgcggcagtg
     2521 gcgaatttca atgcccaagg cactgacgtt gcggaaatct gccattgata aatcacagct
     2581 tttgaaaatg gcatctttca gcattgcgcg actaaaattg caccctttct gactttcgcg
     2641 atcatagaac tggcagccga taaattcagt gccgctcagg tcggcacctg aaaaatcgca
     2701 gttaaaaaat gtactatttt caactttctc accggtgaag cgatttctgt caattttttc
     2761 gccaactaat gccagagtca tatctatgcc tgtgttttta tacagtaatg acgccatggt
     2821 aaaccttatg acgttatgcg tcaaacccgc cagtatgaca cctgcaaatg cgtattagat
     2881 ctggtgtttc ttcagcaagg cacgtagctg atgataagtc agacccagta attcagcggc
     2941 ttttttctgg ttaaatttag cctgctgtaa gctggtttgt agaaagtctt tctcttgctg
     3001 ctgctggaat tcacgcagat ccagcgttat aacgatgtgt atcctatgtg ctcggattgg
     3061 atagatttcc attacggtga ag
//
