LOCUS       NC_019080               9007 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli plasmid pT108, complete sequence.
ACCESSION   NC_019080
VERSION     NC_019080.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226127
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9007)
  AUTHORS   Yatsomboon,A., Jitwasinkul,T., Tribuddharat,C., Srifuengfung,S.,
            Vorachit,M. and Dhiraputra,C.
  TITLE     Two almost identical plasmids carrying Escherichia coli ampC
            beta-lactamase gene from Thailand and Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9007)
  AUTHORS   Yatsomboon,A., Jitwasinkul,T., Tribuddharat,C., Srifuengfung,S.,
            Vorachit,M. and Dhiraputra,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2011) Department of Microbiology, Faculty of
            Medicine Siriraj Hospital, Mahidol University, 2 Prannok Rd.,
            Bangkoknoi, Bangkok 10700, Thailand
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009957865.2-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 01:50:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 8
            CDSs (with protein)               :: 8
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JN412137.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="108"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pT108"
                     /geo_loc_name="Thailand"
                     /note="clinical isolate"
     gene            complement(join(7834..9007,1..320))
                     /locus_tag="HS830_RS00005"
     CDS             complement(join(7834..9007,1..320))
                     /locus_tag="HS830_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="WP_001120888.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMAE
                     QGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARRMA
                     ESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKKAGH
                     THQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPELTQL
                     THTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQAGRKV
                     FTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLT
                     RGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALV
                     TPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIED
                     PIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
                     /name="IS91-like element ISVsa3 family transposase"
     gene            complement(364..786)
                     /locus_tag="HS830_RS00010"
     CDS             complement(364..786)
                     /locus_tag="HS830_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            971..1411
                     /locus_tag="HS830_RS00015"
                     /old_locus_tag="D616_p70002"
     CDS             971..1411
                     /locus_tag="HS830_RS00015"
                     /old_locus_tag="D616_p70002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(2178..2327)
                     /locus_tag="HS830_RS00020"
     CDS             complement(2178..2327)
                     /locus_tag="HS830_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            2898..3056
                     /locus_tag="HS830_RS00055"
     CDS             2898..3056
                     /locus_tag="HS830_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            complement(<3359..4276)
                     /locus_tag="HS830_RS00025"
                     /pseudo=""
     CDS             complement(<3359..4276)
                     /locus_tag="HS830_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /name="IS4-like element ISVsa5 family transposase"
     gene            4408..5550
                     /gene="ampC"
                     /locus_tag="HS830_RS00030"
                     /old_locus_tag="D616_p70003"
     CDS             4408..5550
                     /gene="ampC"
                     /locus_tag="HS830_RS00030"
                     /old_locus_tag="D616_p70003"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMY2/MIR/ACT/EC family class C beta-lactamase"
                     /protein_id="WP_011666807.1"
                     /translation="MLKTTLCALLITASCSTLAASFSEQQINDIVHRTITPLVEQQKIP
                     GIAVAVIYQGKPYYFTWGYADVTQKRPVTQQTLFELGSVSKTFTGVLSGDAIARGEINL
                     SDHVTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSATDLLRFYQNWQPEWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPPAEEKNYAWGY
                     REGKAVHVSPGMLDAEAYGVKSTIEDMARWVRSNINPRDINDKTLQQGIQLAQSRYWQT
                     GDMYQGLGWEMLDWPVDPDSIINGSDSKIALAARPVKAMMPPTPAVRSSWVHKTGATGG
                     FGSYIAFIPEKELGIVMLANKNYPNPARVTAAWQILNALQ"
                     /name="ampC"
     gene            5639..6172
                     /gene="blc"
                     /locus_tag="HS830_RS00035"
                     /old_locus_tag="D616_p70004"
     CDS             5639..6172
                     /gene="blc"
                     /locus_tag="HS830_RS00035"
                     /old_locus_tag="D616_p70004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="WP_011666806.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWYE
                     IARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRAAL
                     KVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVATREG
                     FDISKLIWVQQPGS"
                     /name="blc"
     gene            complement(6169..>6291)
                     /gene="sugE"
                     /locus_tag="HS830_RS00040"
                     /pseudo=""
     CDS             complement(6169..>6291)
                     /gene="sugE"
                     /locus_tag="HS830_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /name="sugE"
     gene            6454..7269
                     /gene="sul2"
                     /locus_tag="HS830_RS00045"
                     /old_locus_tag="D616_p70005"
     ARG             6455..7266
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7356..>7598
                     /gene="glmM"
                     /locus_tag="HS830_RS00050"
                     /pseudo=""
     CDS             7356..>7598
                     /gene="glmM"
                     /locus_tag="HS830_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891390.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /name="glmM"
     ARG             4409..5550
                     /name="Escherichia coli ampC beta-lactamase"
                     /gene="Escherichia coli ampC beta-lactamase"
                     /gene_name="ampC-type beta-lactamase"
                     /tool="rgi"
                     /drug_class="cephalosporin; penam"
                     /identity="91.32"
                     /coverage="100.8"
     Typing          complement(2363..2536)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          2460..3065
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcactttcgg ccatccgccg cgccccacag ctcgggcaga aaccgcgacg cttacagctg
       61 aaagcgacca ggtgctcggc gtggcaagac tcgcagcgaa cccgtagaaa gccatgctcc
      121 agccgcccgc attggagaaa ttcttcaaat tcccgttgca catagcccgg caattccttt
      181 ccctgctctg ccataagcgc agcgaatgcc gggtaatact cgtcaacgat ctgatagaga
      241 agggtttgct cgggtcggtg gctctggtaa cgaccagtat cccgatcccg gctggccgtc
      301 ctggccgcca catgaggcat gttccgcgtc cttgcaatac tgtgtttaca tacagtctat
      361 cgcttagcgg aaagttcttt taccctcagc cgaaatgcct gccgttgcta gacattgcca
      421 gccagtgccc gtcactccca ctcttacatt gttcgcagta tctaggtaca tgttattgcg
      481 tgcaccttca tgatgtataa tgttattaat gacagcagaa agatcgacag aattaatatt
      541 cttcgtacct gggacagtta tcgggtgtcg gccagtgatg agaccaaagg atgcagtata
      601 agatgaacca ctactattat catggccacc attaccacca ccgttaaaac tacctcccga
      661 acttccagag ccgctatgac catcattgta ggggtcattt ggtcccatgt gaccaaaacc
      721 accgacagta ccgctattta actctgtact cataaaatta tcctctttga cttttaaaaa
      781 cagcaaaaac tgtatttata acaatgtgtt tatatacagt attcgaaata ctacgcccgt
      841 caaaaccaca ccgtcaacag gttttttgtg tatttttcac gcctcacggg cagcctcaga
      901 ccttttgtgt taaaacagta atatgttctg ctgttacaat acggtacaca caggagacac
      961 tgtcactttt atggcatggc acacagttca agagacactg aaattaacag gaaagtcgcg
     1021 ctcgcaatta tatcgtgaca tggctaaagg ccttgtcagc tatcgcacag gaaaaaatga
     1081 tcgccgggag ttcgagacca gtgaactgat tcgggcctat ggcgagctgc gcacttctga
     1141 gacaccaaaa aggcacaagg agggacactc caaatctgtc gttagccagg cggaaaatgc
     1201 tcagctagac gtcattcagc agaaactgga cgctctgcag cagactgtta ccctaatgct
     1261 tgaagacaag atggcgcggg aagcagaaaa ccgcctgcga aatgaggaga gggaacaact
     1321 acaggccgaa attagccgtc tggctgacgc gctggcacag gaaaaaaagc ggggattctg
     1381 gtcgcggctg tttcgctccg gttcagagta acagtgcagg tgactctgat ttaccataac
     1441 atctgttatc agcaccgcct gagaggccct cagggcgttt ctgacgggaa accggtaaaa
     1501 caggcaacaa accacccgaa aacccgccag aaacgcgcag gctctgtttt tacggcatgc
     1561 atgagtatgc gcacaagtgc atagcgcgga aattcacacc attcagccgg ggtaagtcct
     1621 gtttccggcg ttcttcccat ccgaaaacgg tgaacgggga cgcaatttat tgcgtggccg
     1681 tgaagcgttg ccggcgcagc cggaaaaccc gctgtcagca cgttcaggaa gggggtgatg
     1741 tacaaatggc acagaggatg aaagtgacgt atgagctgaa ggaatgatgt aaaagtgatg
     1801 taaaagtgat gtataagtga tgtaaaagtg atgtaaaagt gatgtatgaa ctgaaggagc
     1861 gatgtaattc tgtagtcagg aagaataacc cgttcatgca ggcatgaacg atacagtgag
     1921 gaacgctttt tttgtcacga tctcccctta acctgcgtaa atcacccgcc atggttgatt
     1981 atttttcaac ctcaaaatgg tcatacacct aacgcagccg cgattttcat cgcaactgca
     2041 tgaattaaaa gaaaaaaaag gaatatttcg ttttcacgag taatacccgg gtattaaaaa
     2101 tccactgtaa ttacatgaaa ataaagaaat aaaaggtaat accggagtat taccacgtac
     2161 gcgggcaagc gaaaaagcta taacgcttcc cggccgacag gcgacccaaa atccacaggg
     2221 gcatgcaggt tctcttcagt aacctgcgcc attaaatcaa aaaggtcata ttcgccggga
     2281 agctgcgtat cagtcgaatc cagttgttca tcatattcag atggcatggg gtgatatcca
     2341 gtagctgaga ggttgttagc gggtttcggg gcgcagccct gaaccagtca tgtagcgcta
     2401 gcggagtgta tactggctta ctatgttgcc acgattgcga aaatcagtga agtgcttcat
     2461 gtggcaggag aaaaaaggca gcaccggcgc gtcagcagaa tatgcgatac aggagatatt
     2521 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg
     2581 cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag
     2641 ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa
     2701 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc
     2761 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     2821 cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc
     2881 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg
     2941 gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca
     3001 ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc
     3061 ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa
     3121 gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt
     3181 acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact
     3241 atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta
     3301 acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatagc
     3361 ctagtccgta ggcaggactt ttcaagtctc ggaaggtttc ttcaatctgc attcgcttcg
     3421 aatagatatt aacaagttgt ttgggtgttc gaatttcaac aggtaagtta gttgctagaa
     3481 cccatggctc ctttgccgac gctgagtaga ttttaggtga cgggtggtga caatgagtcc
     3541 gtgtcgagcg ctgatttttt cggcctttag agcgagattt atacaataga atttggcatg
     3601 agattggatt gcttttagtc agcctcttat agcctaaagt ctttgagtga ctagatgaca
     3661 tatcatgtaa gttgctgata ggtttccagt tttccgctcc taggtctgca tattgtactt
     3721 ttcctcttac tcgacttaac cagtaccaac ccagcttctc aacggattta taccatggca
     3781 ctttaaagcc agcatcactg acaatgagcg gtgtggtgtt actcggtaga atgctcgcaa
     3841 ggtcggctag aaattggtca tgagctttct ttgaacattg ctctgaaagc gggaacgctt
     3901 tctcataaag agtaacagaa cgaccgtgta gtgcgactga agctcgcaat accataagtc
     3961 gtttttgctc acgaatatca gaccagtcaa caagtacaat gggcatcgta ttgcccgaac
     4021 agataaagct agcatgccaa cggtatacag cgagtcgctc tttgtggagg tgacgattac
     4081 ctaacaatcg gtcgattcgt ttgatgttat gttttgttct cgctttggtt ggcaggttac
     4141 ggccaagttc ggtaagagtg agagttttac agtcaagtaa tgcgtggcaa gccaacgtta
     4201 agctgttgag tcgttttaag tgtaattcgg ggcagaattg gtaaagagag tcgtgtaaaa
     4261 tatcgagttc gcacatcttg ttgtctgatt attgattttt cgcgaaacca tttgatcata
     4321 tgacaagatg tgtatccacc ttaacttaat gatttttacc aaaatcatta ggggattcat
     4381 cagcgttttg tatggaaatc agaccttatg ctcaaaacga cgctctgcgc cttactgatt
     4441 actgcctctt gctcaacact tgccgcctcc ttcagtgaac aacaaatcaa cgatattgtg
     4501 catcgcacga ttaccccact tgttgagcaa caaaaaatcc ccggcatagc cgttgctgtg
     4561 atttatcagg gcaagcctta ttactttacc tggggctatg cggacgtgac acaaaagcgg
     4621 cccgtcacgc agcaaacgtt gtttgagtta ggttcggtca gtaaaacatt taccggcgtg
     4681 cttagtggcg acgccattgc acgaggggaa atcaacctaa gcgatcacgt cacaaaatac
     4741 tggcctgaac ttaccgctaa acagtggaat gggatcacgc tattacatct cgcaacctac
     4801 accgctggcg gtctgccatt gcaggtgccg gatgaagtga agtcagcgac tgacctgcta
     4861 cgtttctatc aaaactggca gcctgaatgg gctccaggaa cacaacgttt atacgcgaac
     4921 tccagtattg gtttgtttgg cgcactggct gtgaagccgt ctggtttgag ctttgaacag
     4981 gcgatgcaaa ctcgtgtttt ccagccactc aaactcaccc atacgtggat taacgtgccg
     5041 cccgcagaag aaaagaatta cgcctgggga tatcgcgaag gtaaggcagt acatgtttcg
     5101 ccagggatgt tagatgctga agcttacggt gtgaaatcaa caattgaaga tatggctcgc
     5161 tgggtgcgga gcaatataaa tccgcgtgat atcaacgaca aaacacttca acaagggata
     5221 caactggcgc aatctcgcta ctggcagacc ggcgatatgt atcagggcct gggctgggaa
     5281 atgctggact ggccggtaga tcctgacagc atcattaacg gcagcgacag taaaattgcg
     5341 ctggcggcac gaccagtaaa agcgatgatg ccaccaactc ctgctgtgcg gtcatcatgg
     5401 gtgcataaaa caggcgcgac cggcggattt ggcagctaca tcgcatttat cccagaaaaa
     5461 gagctgggca ttgtgatgct ggctaacaaa aactatccca atccagcgag agttaccgcc
     5521 gcctggcaga ttctcaacgc tctacagtaa aatttcatcg ggtccgaatt ttcggacctt
     5581 ttctccgctt ttccttgctg tcatctacac ttagaaaaaa accagtaagg aaacaattat
     5641 gcgcctgctc cctctcgttg ccgcagcaac agctgcattt ctggtcgttg cctgcagttc
     5701 tcccacgccg ccgcgtggtg tgaccgtagt gaataatttc gatgctaaac gttatctcgg
     5761 tacctggtat gagattgccc gttttgatca ccgctttgaa cgtggactgg agaaagtcac
     5821 cgcaacatac agcctacggg atgatggcgg cctgaatgtc atcaataaag gctataaccc
     5881 tgatagagaa atgtggcagc agagtgaagg gaaagcgtac tttaccggcg cgccaactcg
     5941 cgctgcgctg aaagtatcat tctttggtcc tttctatggc ggttataacg tcattgcact
     6001 cgatcgggag tatcgccatg cgctggtttg cggcccggac cgcgactacc tgtggatact
     6061 ctcccgcacg ccaactattt ctgacgaagt aaaacaggag atgctggcag tcgcgacccg
     6121 ggaagggttt gatatcagta agttgatttg ggtacaacag cccggtagtt agtgagtgct
     6181 gagtttcaga ccaataatcc ccagtacgat taacgccaga ctcgccaggc gcatcgggtt
     6241 agcggactcg ccgagcagca caatgccggt gatggccgcg ccgactgcgc catatatcta
     6301 cttattgaga gatgatatat ttatttccaa aatgctacag atacatcatc atgaaacgga
     6361 tcaccgcaaa caggttactc gcctggggat tccctttcga cccgagcatc cgtatgagac
     6421 tcatgctcga ttattattat tatagaagcc cccatgaata aatcgctcat cattttcggc
     6481 atcgtcaaca taacctcgga cagtttctcc gatggaggcc ggtatctggc gccagacgca
     6541 gccattgcgc aggcgcgtaa gctgatggcc gagggggcag atgtgatcga cctcggtccg
     6601 gcatccagca atcccgacgc cgcgcctgtt tcgtccgaca cagaaatcgc gcgtatcgcg
     6661 ccggtgctgg acgcgctcaa ggcagatggc attcccgtct cgctcgacag ttatcaaccc
     6721 gcgacgcaag cctatgcctt gtcgcgtggt gtggcctatc tcaatgatat tcgcggtttt
     6781 ccagacgctg cgttctatcc gcaattggcg aaatcatctg ccaaactcgt cgttatgcat
     6841 tcggtgcaag acgggcaggc agatcggcgc gaggcacccg ctggcgacat catggatcac
     6901 attgcggcgt tctttgacgc gcgcatcgcg gcgctgacgg gtgccggtat caaacgcaac
     6961 cgccttgtcc ttgatcccgg catggggttt tttctggggg ctgctcccga aacctcgctc
     7021 tcggtgctgg cgcggttcga tgaattgcgg ctgcgcttcg atttgccggt gcttctgtct
     7081 gtttcgcgca aatcctttct gcgcgcgctc acaggccgtg gtccggggga tgtcggggcc
     7141 gcgacactcg ctgcagagct tgccgccgcc gcaggtggag ctgacttcat ccgcacacac
     7201 gagccgcgcc ccttgcgcga cgggctggcg gtattggcgg cgctgaaaga aaccgcaaga
     7261 attcgttaac tgcacattcg ggatatttct ctatattcgc ggttcagcag gcatgtcccc
     7321 tttgagggcg acccgacgac aggataatcg accttatggt gcgcaaatat ttcggcacag
     7381 acggtattcg tggcaaagcc aacgaaggcg cgatgacggc ggaaaccgcc ttgcgcgtcg
     7441 gcatggcggc tggccgtgtc tttcgtcgcg gtgaccaccg ccatcgtgtc gtgatcggca
     7501 aggatacgcg cctgtcgggc tatatgcttg aacccgcgct cacagccggt ttcacctcga
     7561 tgggcatgga cgtattcctt tttggcccgc tgccgacaac gtataggaag aataaacgcc
     7621 cttttcaccc aagtccaaca gctttggacc acagttgact ctttcgacac ccctgcgatg
     7681 caacccaatc cggctgacgg ggagccagca acgctgaaaa tttaccctcc tctttcccac
     7741 tagcggctcc ttttccgaca accagcacgg cggatccctg ccgcggcgct gtgaacgcag
     7801 cattttgatt ggtatcgttg gccttcaggc tcgtcagtca aacagaccca ggagcagctc
     7861 agccggtggc gcccggcttt cgggtaacgc cctggtcccg ctggtttcgg ctttgtgctt
     7921 caggtgatca aggatctgct tgatcactat agggtcttca atgcaggcga tgactttcat
     7981 ggcgccgccg cagccgctgc aggtctcgat gtcgatattg aaaacacgct tgagccgttg
     8041 cgcccatgtc atcgacgctc gccgttgtgc tggtgttgcc ggttcatcag ccaccctgac
     8101 cttgttgccc ctgccccgtt ttgccggcgt gaccaacgcc cggtgccgac tgttgggtgc
     8161 gaacaccccg tggaagcggg ttaggttgac tctgggcttc ggtaccaggg cggccagcct
     8221 tgcaatgaaa tccaatggtt cgaaaatgac gtgcgtggtg ccgtcccggt acggcgtctt
     8281 gagctggtag cgcacgttgc cgcctcgtgt taacgacagc cgcttctcgg ataccgccgg
     8341 gcggctgatg taccggcaca gccgttcgag cttcttgcgt tcatcggccc tggccgccac
     8401 gccggcgtgc aggctggacc cggctacctt gccaatcccg tcaccgaacg gatcaccact
     8461 ggtcggcaga gtttgcaaag tgaacacctt tcgccccgcc tgtgaaccga cagcgatacg
     8521 gtaagtgatc gagtgcccca gcaggggtgt catcgggtcg tcatccaccg catccgaggc
     8581 cagatagctg ttttcgacat cccgttccag caggccttgc cgttccagat agcgacccac
     8641 ccggtgggcg atggtgtgcg tcagctgggt gagctctggg ctggtcggcg ccttgaccca
     8701 gcggaaacgc gctgagccgt gggattgctc gacatacaca ccgtcgagaa acagcatgtg
     8761 gaagtgaaca ttcagattga gcgccgatcc aaaacgctgg atcagggtga ccgcgcccgt
     8821 cttggccact tggtgggtat ggcccgcttt cttgaccagg tgcgtggcaa tgacgcggta
     8881 aacgatgccc agcacccacc ccatgatctc gggccggctg gcaaacagga aacgcagctg
     8941 aaacgggaag ctcaacaccc actgacgcat gggttgttca ggcagtactt catcaaccag
     9001 caaggcg
//
