LOCUS       NC_019070               6790 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli plasmid pCERC1, complete sequence.
ACCESSION   NC_019070
VERSION     NC_019070.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226099
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Anantham,S. and Hall,R.M.
  TITLE     pCERC1, a small, globally disseminated plasmid carrying the dfrA14
            cassette in the strA gene of the sul2-strA-strB gene cluster
  JOURNAL   Microb Drug Resist 18 (4), 364-371 (2012)
   PUBMED   22416992
REFERENCE   2  (bases 1 to 6790)
  AUTHORS   Anantham,S. and Hall,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2011) School of Molecular Bioscience, The
            University of Sydney, Building G08, Camperdown, NSW 2006, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009957545.2-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 03:09:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10
            CDSs (total)                      :: 10
            Genes (coding)                    :: 7
            CDSs (with protein)               :: 7
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JN012467.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S1.2.T2R"
                     /isolation_source="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pCERC1"
                     /note="human commensal Escherichia coli"
     gene            97..912
                     /gene="sul2"
                     /locus_tag="HS799_RS00005"
                     /old_locus_tag="D616_p61006"
     ARG             98..909
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            973..>1500
                     /locus_tag="HS799_RS00010"
                     /old_locus_tag="D616_p61005"
                     /pseudo=""
     CDS             973..>1500
                     /locus_tag="HS799_RS00010"
                     /old_locus_tag="D616_p61005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /name="phosphotransferase"
     gene            1521..1994
                     /gene="dfrA14"
                     /locus_tag="HS799_RS00015"
                     /old_locus_tag="D616_p61003"
     ARG             1522..1991
                     /name="dfrA14"
                     /gene="dfrA14"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            <2069..2344
                     /locus_tag="HS799_RS00020"
                     /old_locus_tag="D616_p61004"
                     /pseudo=""
     CDS             <2069..2344
                     /locus_tag="HS799_RS00020"
                     /old_locus_tag="D616_p61004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
                     /name="aminoglycoside phosphotransferase APH(3')"
     gene            2344..3180
                     /locus_tag="HS799_RS00025"
                     /old_locus_tag="D616_p61002"
     CDS             2344..3180
                     /locus_tag="HS799_RS00025"
                     /old_locus_tag="D616_p61002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(<3287..3691)
                     /locus_tag="HS799_RS00030"
                     /old_locus_tag="D616_p61001"
                     /pseudo=""
     CDS             complement(<3287..3691)
                     /locus_tag="HS799_RS00030"
                     /old_locus_tag="D616_p61001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(3735..4157)
                     /locus_tag="HS799_RS00035"
                     /old_locus_tag="D616_p61010"
     CDS             complement(3735..4157)
                     /locus_tag="HS799_RS00035"
                     /old_locus_tag="D616_p61010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            4342..4782
                     /locus_tag="HS799_RS00040"
                     /old_locus_tag="D616_p61009"
     CDS             4342..4782
                     /locus_tag="HS799_RS00040"
                     /old_locus_tag="D616_p61009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(5549..5698)
                     /locus_tag="HS799_RS00045"
                     /old_locus_tag="D616_p61007"
     CDS             complement(5549..5698)
                     /locus_tag="HS799_RS00045"
                     /old_locus_tag="D616_p61007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            6269..6427
                     /locus_tag="HS799_RS00050"
     CDS             6269..6427
                     /locus_tag="HS799_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     ARG             2345..3177
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             2100..2344
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="30.34"
     ARG             974..1515
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.43"
                     /coverage="67.42"
     Typing          complement(5734..5907)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          5831..6436
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggatcaccgc aaacaggtta ctcgcctggg gattcccttt cgacccgagc atccgtatga
       61 gactcatgct cgattattat tattatagaa gcccccatga ataaatcgct catcattttc
      121 ggcatcgtca acataacctc ggacagtttc tccgatggag gccggtatct ggcgccagac
      181 gcagccattg cgcaggcgcg taagctgatg gccgaggggg cagatgtgat cgacctcggt
      241 ccggcatcca gcaatcccga cgccgcgcct gtttcgtccg acacagaaat cgcgcgtatc
      301 gcgccggtgc tggacgcgct caaggcagat ggcattcccg tctcgctcga cagttatcaa
      361 cccgcgacgc aagcctatgc cttgtcgcgt ggtgtggcct atctcaatga tattcgcggt
      421 tttccagacg ctgcgttcta tccgcaattg gcgaaatcat ctgccaaact cgtcgttatg
      481 cattcggtgc aagacgggca ggcagatcgg cgcgaggcac ccgctggcga catcatggat
      541 cacattgcgg cgttctttga cgcgcgcatc gcggcgctga cgggtgccgg tatcaaacgc
      601 aaccgccttg tccttgatcc cggcatgggg ttttttctgg gggctgctcc cgaaacctcg
      661 ctctcggtgc tggcgcggtt cgatgaattg cggctgcgct tcgatttgcc ggtgcttctg
      721 tctgtttcgc gcaaatcctt tctgcgcgcg ctcacaggcc gtggtccggg ggatgtcggg
      781 gccgcgacac tcgctgcaga gcttgccgcc gccgcaggtg gagctgactt catccgcaca
      841 cacgagccgc gccccttgcg cgacgggctg gcggtattgg cggcgctgaa agaaaccgca
      901 agaattcgtt aactgcacat tcgggatatt tctctatatt cgcgcttcat cagaaaactg
      961 aaggaacctc cattgaatcg aactaatatt ttttttggtg aatcgcattc tgactggttg
     1021 cctgtcagag gcggagaatc tggtgatttt gtttttcgac gtggtgacgg gcatgccttc
     1081 gcgaaaatcg cacctgcttc ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt
     1141 tggctcaaag gtcgaggtgt ggcttgcccc gaggtcatca actggcagga ggaacaggag
     1201 ggtgcatgct tggtgataac ggcaattccg ggagtaccgg cggctgatct gtctggagcg
     1261 gatttgctca aagcgtggcc gtcaatgggg cagcaacttg gcgctgttca cagcctatcg
     1321 gttgatcaat gtccgtttga gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg
     1381 gtgtcccgca atgccgtcaa tcccgacttc ttaccggacg aggacaagag tacgccgcag
     1441 ctcgatcttt tggctcgtgt cgaacgagag ctaccggtgc ggctcgacca agagcgcacc
     1501 gttaacccag gatgagaacc ttgaaagtat cattgatagc tgcgaaagcg aaaaacggcg
     1561 tgattggttg cggtccagac ataccctggt ccgcgaaagg ggagcagcta ctttttaaag
     1621 cattgaccta caatcagtgg cttctggtgg gtcgcaagac gtttgaatct atgggcgcac
     1681 tccccaatag gaaatacgcg gtcgttaccc gctcaggttg gacatcaaat gatgacaatg
     1741 tagttgtatt tcagtcaatc gaagaggcca tggacaggct agctgaattc accggtcacg
     1801 ttatagtgtc tggtggcgga gaaatttacc gagaaacatt acccatggcc tctacgctcc
     1861 acttatcgac gatcgacatc gagccagagg gggatgtttt cttcccgagt attccaaata
     1921 ccttcgaagt tgtttttgag caacacttta cttcaaacat taactattgc tatcaaattt
     1981 ggaaaaaggg ttaacaaagc tatgcaatcg acggcaaaaa gcttcgttcg cttcgcgcac
     2041 tacgcctttt tccgcgattg atagcgacga tatggttgtt tgccatggtg atccctgcat
     2101 gccgaacttc atggtggacc ctaaaactct tcaatgcacg ggtctgatcg accttgggcg
     2161 gctcggaaca gcagatcgct atgccgattt ggcactcatg attgctaacg ccgaagagaa
     2221 ctgggcagcg ccagatgaag cagagcgcgc cttcgctgtc ctattcaatg tattggggat
     2281 cgaagccccc gaccgcgaac gccttgcctt ctatctgcga ttggaccctc tgacttgggg
     2341 ttgatgttca tgccgcctgt ttttcctgct cattggcacg tttcgcaacc tgttctcatt
     2401 gcggacacct tttccagcct cgtttggaaa gtttcattgc cagacgggac tcctgcaatc
     2461 gtcaagggat tgaaacctat agaagacatt gctgatgaac tgcgcggggc cgactatctg
     2521 gtatggcgca atgggagggg agcagtccgg ttgctcggtc gtgagaacaa tctgatgttg
     2581 ctcgaatatg ccggggagcg aatgctctct cacatcgttg ccgagcacgg cgactaccag
     2641 gcgaccgaaa ttgcagcgga actaatggcg aagctgtatg ccgcatctga ggaacccctg
     2701 ccttctgccc ttctcccgat ccgggatcgc tttgcagctt tgtttcagcg ggcgcgcgat
     2761 gatcaaaacg caggttgtca aactgactac gtccacgcgg cgattatagc cgatcaaatg
     2821 atgagcaatg cctcggaact gcgtgggcta catggcgatc tgcatcatga aaacatcatg
     2881 ttctccagtc gcggctggct ggtgatagat cccgtcggtc tggtcggtga agtgggcttt
     2941 ggcgccgcca atatgttcta cgatccggct gacagagacg acctttgtct cgatcctaga
     3001 cgcattgcac agatggcgga cgcattctct cgtgcgctgg acgtcgatcc gcgtcgcctg
     3061 ctcgaccagg cgtacgctta tgggtgcctt tccgcagctt ggaacgcgga tggagaagag
     3121 gagcaacgcg atctagctat cgcggccgcg atcaagcagg tgcgacagac gtcatactag
     3181 atatcaagcg acttctccta tcccctggga acacatcaat ctcaccggag aatatcgctg
     3241 gccaaagcct tagcgtagga ttccgcccct tcccgcaaac gaccccaaac aggaaacgca
     3301 gctgaaacgg gaagctcaac acccactgac gcatgggttg ttcaggcagt acttcatcaa
     3361 ccagcaaggc ggcactttcg gccatccgcc gcgccccaca gctcgggcag aaaccgcgac
     3421 gcttacagct gaaagcgacc aggtgctcgg cgtggcaaga ctcgcagcga acccgtagaa
     3481 agccatgctc cagccgcccg cattggagaa attcttcaaa ttcccgttgc acatagcccg
     3541 gcaattcctt tccctgctct gccataagcg cagcgaatgc cgggtaatac tcgtcaacga
     3601 tctgatagag aagggtttgc tcgggtcggt ggctctggta acgaccagta tcccgatccc
     3661 ggctggccgt cctggccgcc acatgaggca tgttccgcgt ccttgcaata ctgtgtttac
     3721 atacagtcta tcgcttagcg gaaagttctt ttaccctcag ccgaaatgcc tgccgttgct
     3781 agacattgcc agccagtgcc cgtcactccc actcttacat tgttcgcagt atctaggtac
     3841 atgttattgc gtgcaccttc atgatgtata atgttattaa tgacagcaga aagatcgaca
     3901 gaattaatat tcttcgtacc tgggacagtt atcgggtgtc ggccagtgat gagaccaaag
     3961 gatgcagtat aagatgaacc actactatta tcatggccac cattaccacc accgttaaaa
     4021 ctacctcccg aacttccaga gccgctatga ccatcattgt aggggtcatt tggtcccatg
     4081 tgaccaaaac caccgacagt accgctattt aactctgtac tcataaaatt atcctctttg
     4141 acttttaaaa acagcaaaaa ctgtatttat aacaatgtgt ttatatacag tattcgaaat
     4201 actacgcccg tcaaaaccac accgtcaaca ggttttttgt gtatttttca cgcctcacgg
     4261 gcagcctcag accttttgtg ttaaaacagt aatatgttct gctgttacaa tacggtacac
     4321 acaggagaca ctgtcacttt tatggcatgg cacacagttc aagagacact gaaattaaca
     4381 ggaaagtcgc gctcgcaatt atatcgtgac atggctaaag gccttgtcag ctatcgcaca
     4441 ggaaaaaatg atcgccggga gttcgagacc agtgaactga ttcgggccta tggcgagctg
     4501 cgcacttctg agacaccaaa aaggcacaag gagggacact ccaaatctgt cgttagccag
     4561 gcggaaaatg ctcagctaga cgtcattcag cagaaactgg acgctctgca gcagactgtt
     4621 accctaatgc ttgaagacaa gatggcgcgg gaagcagaaa accgcctgcg aaatgaggag
     4681 agggaacaac tacaggccga aattagccgt ctggctgacg cgctggcaca ggaaaaaaag
     4741 cggggattct ggtcgcggct gtttcgctcc ggttcagagt aacagtgcag gtgactctga
     4801 tttaccataa catctgttat cagcaccgcc tgagaggccc tcagggcgtt tctgacggga
     4861 aaccggtaaa acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt
     4921 ttacggcatg catgagtatg cgcacaagtg catagcgcgg aaattcacac cattcagccg
     4981 gggtaagtcc tgtttccggc gttcttccca tccgaaaacg gtgaacgggg acgcaattta
     5041 ttgcgtggcc gtgaagcgtt gccggcgcag ccggaaaacc cgctgtcagc acgttcagga
     5101 agggggtgat gtacaaatgg cacagaggat gaaagtgacg tatgagctga aggaatgatg
     5161 taaaagtgat gtataagtga tgtaaaagtg atgtataagt gatgtaaaag tgatgtatga
     5221 actgaaggag cgatgtaatt ctgtagtcag gaagaataac ccgttcatgc aggcatgaac
     5281 gatacagtga ggaacgcttt ttttgtcacg atctcccctt aacctgcgta aatcacccgc
     5341 catggttgat tatttttcaa cctcaaaatg gtcatacacc taacgcagcc gcgattttca
     5401 tcgcaactgc atgaattaaa agaaaaaaaa ggaatatttc gttttcacga gtaatacccg
     5461 ggtattaaaa atccactgta attacatgaa aataaagaaa taaaaggtaa taccggagta
     5521 ttaccacgta cgcgggcaag cgaaaaagct ataacgcttc ccggccgaca ggcgacccaa
     5581 aatccacagg ggcatgcagg ttctcttcag taacctgcgc cattaaatca aaaaggtcat
     5641 attcgccggg aagctgcgta tcagtcgaat ccagttgttc atcatattca gatggcatgg
     5701 ggtgatatcc agtagctgag aggttgttag cgggtttcgg ggcgcagccc tgaaccagtc
     5761 atgtagcgct agcggagtgt atactggctt actatgttgc cacgattgcg aaaatcagtg
     5821 aagtgcttca tgtggcagga gaaaaaaggc agcaccggcg cgtcagcaga atatgcgata
     5881 caggagatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc ggctgcggcg
     5941 agcggtaccg gcttacggac ggggacggaa atttcctgga agataccagg aagacactta
     6001 gcaaagaaga gataaggcca cggcgaagcc gtttttccat aggctccgcc cccctgacaa
     6061 gcatcgcaaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagatc
     6121 ccaggcgttt ccccctggta gctccctcgt gcgctctcct gttcctgcct ttcggtttac
     6181 cggtgtcatt ccgctgttat ggccgcggtt tctcattcca cgcctgacac tcagttccgt
     6241 gaaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg
     6301 cgccttgtcc ggtaactatc aacttgagtc caacccggaa agacacgaca aaacgccact
     6361 ggcagcagcc attagtaaca ggagtgcacg agaagataga ttaagaggtc ttgaagtaga
     6421 ggcctgactg cggctacact gaaagaacag ttttggtaat ggcggtctcg tgaaccctgt
     6481 taccgggtta agcagttccc caactgactt aaccttcgaa caaaccgcct ccccaggcgg
     6541 ttttttcgtt tacagagcaa gagattacga ttcattgaaa aggatctcaa gaagatcctt
     6601 tgtagattac tatcaattaa tcttccgaac atgacaccag tcactgttga ttacggttaa
     6661 accgctccgt aacaggtttc cctcgtattg gtgacttgca gcaccaaaga agcaaaaaag
     6721 ataaatatat ctacttattg agagatgata tatttatttc caaaatgcta cagatacatc
     6781 atcatgaaac
//
