LOCUS       NC_019056               9902 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli plasmid pAm08CD9902, complete sequence.
ACCESSION   NC_019056
VERSION     NC_019056.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225349
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9902)
  AUTHORS   Yu,C., Tan,X., Chen,Q., Luo,M. and Zhang,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2009) College of Life Sciences, Sichuan
            University, 29# Wangjiang Road, Chengdu, Sichuan 610064, China
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009953715.1-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 07:01:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 13
            CDSs (total)                      :: 13
            Genes (coding)                    :: 11
            CDSs (with protein)               :: 11
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            GQ149344.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolation_source="intestinal tract"
                     /host="giant panda"
                     /db_xref="taxon:562"
                     /plasmid="pAm08CD9902"
     gene            375..935
                     /locus_tag="HS606_RS00005"
                     /old_locus_tag="D616_p87001"
     CDS             375..935
                     /locus_tag="HS606_RS00005"
                     /old_locus_tag="D616_p87001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001161490.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQL
                     EALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATAGEP
                     KAQLAREFNISRETLYQYLRTDD"
                     /name="recombinase family protein"
     gene            939..>1310
                     /locus_tag="HS606_RS00010"
                     /pseudo=""
     CDS             939..>1310
                     /locus_tag="HS606_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     gene            1290..1535
                     /locus_tag="HS606_RS00015"
     CDS             1290..1535
                     /locus_tag="HS606_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_041937891.1"
                     /translation="MSDFRALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="hypothetical protein"
     gene            complement(1567..2217)
                     /gene="tetR(A)"
                     /locus_tag="HS606_RS00020"
                     /old_locus_tag="D616_p87003"
     CDS             complement(1567..2217)
                     /gene="tetR(A)"
                     /locus_tag="HS606_RS00020"
                     /old_locus_tag="D616_p87003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_038989485.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEARPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     ARG             2249..3522
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="99.28"
                     /coverage="100.0"
     ARG             2324..3519
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="99.75"
                     /coverage="100.0"
     gene            complement(3554..4438)
                     /locus_tag="HS606_RS00030"
                     /old_locus_tag="D616_p87005"
     CDS             complement(3554..4438)
                     /locus_tag="HS606_RS00030"
                     /old_locus_tag="D616_p87005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="WP_000058717.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPA
                     GLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVV
                     FISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLT
                     RKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYF
                     LWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSN
                     RTPRARIACRKSP"
                     /name="EamA family transporter"
     gene            complement(4576..4968)
                     /locus_tag="HS606_RS00035"
                     /old_locus_tag="D616_p87006"
     CDS             complement(4576..4968)
                     /locus_tag="HS606_RS00035"
                     /old_locus_tag="D616_p87006"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407380.1"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="WP_001351729.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIER
                     GDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRLVQ
                     DCCYDPDRDAHEALLRSGFGGRVQVV"
                     /name="isochorismatase family cysteine hydrolase"
     gene            <4972..5837
                     /locus_tag="HS606_RS00040"
                     /pseudo=""
     CDS             <4972..5837
                     /locus_tag="HS606_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            6768..6959
                     /locus_tag="HS606_RS00045"
                     /old_locus_tag="D616_p87008"
     CDS             6768..6959
                     /locus_tag="HS606_RS00045"
                     /old_locus_tag="D616_p87008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7181..7606
                     /locus_tag="HS606_RS00050"
                     /old_locus_tag="D616_p87009"
     CDS             7181..7606
                     /locus_tag="HS606_RS00050"
                     /old_locus_tag="D616_p87009"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181411501.1"
                     /translation="MILAKKRPFPGKGHARMTLWVQRLHINYKIASAFTPERGCTTELH
                     QKYIKITSLLRQIAGVQQNYIKITSLLRHFYIIFTSDLTSELFVHPPLFLGLLHVLKMV
                     FPAVPATPHGHAINCASVHRYRGGSWWLSGRSSDFSR"
                     /name="hypothetical protein"
     gene            complement(7760..7978)
                     /locus_tag="HS606_RS00055"
     CDS             complement(7760..7978)
                     /locus_tag="HS606_RS00055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181411502.1"
                     /translation="MLLTEMEISNIRNITLIARRLAWINAKRTHIDIPSFAELLFSGQK
                     ALFFEQYNYFHSRRYTTAGTSKPPDLP"
                     /name="hypothetical protein"
     gene            complement(8232..8929)
                     /locus_tag="HS606_RS00060"
                     /old_locus_tag="D616_p87010"
     CDS             complement(join(8232..8680,8680..8929))
                     /locus_tag="HS606_RS00060"
                     /old_locus_tag="D616_p87010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            9042..9902
                     /locus_tag="HS606_RS00065"
                     /old_locus_tag="D616_p87011"
     CDS             9042..9902
                     /locus_tag="HS606_RS00065"
                     /old_locus_tag="D616_p87011"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     ARG             9043..9899
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(6274..6893)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.243"
                     /coverage="99.0"
                     /evalue="1.1799999999999999e-174"
ORIGIN
        1 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
       61 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
      121 ttttcgttcc actgagcgtc agaccccgga aacgataggt gcaagcaagt taagggttgc
      181 atcgcgcatg tcaatctagg ctatacccta acttgatgtc aggcagggcc gcgccgcttc
      241 gtcagaatag agtctgcttt cccatttttt gacacatgcc cgcgaaggtt atagatttca
      301 gcctgacaga aatgggcttt gaggcacaac ggaacagaaa gtgcacttaa gccgccttca
      361 accaaggaga catcgtgcag gggcaccgca tcggctacgt ccgggtcagc agctttgacc
      421 agaacccgga acgccagctg gaacaaaccc aggtgagcaa ggtgttcacc gacaaggcat
      481 cgggcaagga cacccagcgc ccccagctcg aagcgctgct gagcttcgtc cgcgaaggcg
      541 atacagtggt ggtgcacagc atggatcggc tggcccgcaa cctcgatgac ctgcgtcgct
      601 tggtacagaa gctgactcag cgcggcgtgc gcatcgagtt cctgaaggag ggcctggtgt
      661 tcactggcga ggactcgccg atggccaacc tgatgctgtc ggtgatgggg gccttcgctg
      721 agttcgagcg cgccctgatc cgcgagcggc agcgtgaggg catcgccttg gccaagcagc
      781 gtggcgcgta ccggggccgc aagaaagccc tgtccgatga gcaggctgct accctgcggc
      841 agcgagcgac ggccggcgag cccaaggcgc agcttgcccg cgagttcaac atcagccggg
      901 aaaccctcta ccagtacctc cgcacggacg actgacacat gccgcgtcgc ttgatcctct
      961 cggccacgga gcgggacacc ctgcttgcgc tgccggaaag ccaggatgac ctgatccgct
     1021 actacacctt caacgactcc gacctgtcgc tgatccgcca gcgacgcggc gacgccaacc
     1081 gcctcggctt cgccgtgcag ctctgcctgc tgcgctaccc cggttacgcg ctgggaaccg
     1141 acagcgagct gcccgagccg gtcatcctgt gggtggcgaa gcaagtccag gccgagccgg
     1201 cgagctgggc aaagtacggc gagcgcgacg tgacccgtcg cgagcatgcc caggaactgc
     1261 gcacctacct gcaactggcc ccgttcggcc tgtccgactt ccgcgccctg gacctcgccc
     1321 gcgagcgcca ggcgcacgag gccggcgcgc ggacccgcgc cacggcccac gagcggacgc
     1381 cgcagcagga gcgccagaag gccgccagag aggccgagcg cggccgtgag gcttggacgc
     1441 tagggcaggg catgaaaaag cccgtagcgg gctgctacgg gcgtctgacg cggtggaaag
     1501 ggggagggga tgttgtctac atggctctgc tgtagtgagt gggttgcgct ccggcagcgg
     1561 tcctgatcaa tcgtcaccct ttctcggtcc ttcaacgttc ctgacaacga gcctcctttt
     1621 cgccaatcca tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg gacgggcttc
     1681 gtcgaaggcg tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt caacggtgcc
     1741 gccgcgctcg ccggcatcgc tgtcgccggc ctgctcctca agcacggccc caacagtgaa
     1801 gtagctgatt gtcatcagcg cattgacggc gtccccggcc gaaaaacccg cctcgcagag
     1861 gaagcgaagc tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg tgccggcatg
     1921 gatgcgcgcg ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg cattcccgat
     1981 cagaaatgag cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat tctccgccag
     2041 catggcttcg gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc agtaaagcgc
     2101 cggctgctga acccccaacc gttccgccag tttgcgtgtc gtcagaccgt ctacgccgac
     2161 ctcgttcaac aggtccaggg cggcacggat cactgtattc ggctgcaact ttgtcatgct
     2221 tgacacttta tcactgataa acataatatg tccaccaact tatcagtgat aaagaatccg
     2281 cgcgttcaat cggaccagcg gaggctggtc cggaggccag acgtgaaacc caacagaccc
     2341 ctgatcgtaa ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct gattatgccg
     2401 gtgctgccgg gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc ccactatggc
     2461 attctgctgg cgctgtatgc gttgatgcaa tttgcctgcg cacctgtgct gggcgcgctg
     2521 tcggatcgtt tcgggcggcg gccggtcttg ctcgtctcgc tggccggcgc tgctgtcgac
     2581 tacgccatca tggcgacggc gcctttcctt tgggttctct atatcgggcg gatcgtggcc
     2641 ggcatcaccg gggcgactgg ggcggtagcc ggcgcttata ttgccgatat cactgatggc
     2701 gatgagcgcg cgcggcactt cggcttcatg agcgcctgtt tcgggttcgg gatggtcgcg
     2761 ggacctgtgc tcggtgggct gatgggcggt ttctcccccc acgctccgtt cttcgccgcg
     2821 gcagccttga acggcctcaa tttcctgacg ggctgtttcc ttttgccgga gtcgcacaaa
     2881 ggcgaacgcc ggccgttacg ccgggaggct ctcaacccgc tcgcttcgtt ccggtgggcc
     2941 cggggcatga ccgtcgtcgc cgccctgatg gcggtcttct tcatcatgca acttgtcgga
     3001 caggtgccgg ccgcgctttg ggtcattttc ggcgaggatc gctttcactg ggacgcgacc
     3061 acgatcggca tttcgcttgc cgcatttggc attctgcatt cactcgccca ggcaatgatc
     3121 accggccctg tagccgcccg gctcggcgaa aggcgggcac tcatgctcgg aatgattgcc
     3181 gacggcacag gctacatcct gcttgccttc gcgacacggg gatggatggc gttcccgatc
     3241 atggtcctgc ttgcttcggg tggcatcgga atgccggcgc tgcaagcaat gttgtccagg
     3301 caggtggatg aggaacgtca ggggcagctg caaggctcac tggcggcgct caccagcctg
     3361 acctcgatcg tcggacccct cctcttcacg gcgatctatg cggcttctat aacaacgtgg
     3421 aacgggtggg catggattgc aggcgctgcc ctctacttgc tctgcctgcc ggcgctgcgt
     3481 cgcgggcttt ggagcggcgc atggcaacga gccgatcgct gatcgtggaa acgataggcc
     3541 tatgccatgc gggtcaaggc gacttccggc aagctatacg cgccctagga gtgcggttgg
     3601 aacgttggcc cagccagata ctcccgatca cgagcaggac gccgatgatt tgaagcgcac
     3661 tcagcgtctg atccaagaac aaccatccta gcaacacggc ggtccccggg ctgagaaagc
     3721 ccagtaagga aacaactgta ggttcgagtc gcgagatccc ccggaaccaa aggaagtagg
     3781 ttaaacccgc tccgatcagg ccgagccacg ccaggccgag aacattggtt cctgtaggca
     3841 tcgggattgg cggatcaaac actaaagcta ctggaacgag cagaagtcct ccggccgcca
     3901 gttgccaggc ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg gtcagcacgg
     3961 ttccgaacgc catggaaacc gcccccgcca ggcccgctgc gacgccgaca ggatctagcg
     4021 ctgcgtttgg tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata gcccccagga
     4081 ccgccatcaa tcgtatcggg ctacctagca gagcggcaga gatgaacacg accatcagcg
     4141 gctgcacagc gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc gaaataaaca
     4201 acaagctcca gaatagcgaa atattaagtg cgccgaggat gaagatgcgc atccaccaga
     4261 ttcccgttgg aatctgtcgg acgatcatca cgagcaataa acccgccggc aacgcccgca
     4321 gcatcgcgac cgtcatcggt gagaagttcg gcaggtattg ggtggtgaca atgtaggtgc
     4381 tgccccaaat ggcaggtgct atcgctgtga acaataaatc gggcgtgcgt aatgacatgc
     4441 gattacaaga cctccgctac ggcgatgggt ttttgagagt tgcaggcgcc gcgatgaccg
     4501 caccctgact tggactggcc ctgcctcgca ccaatccacc tgcggctatg gccgcgatca
     4561 tgcgaggccc gaaaattaca cgacctgtac acgtccgccg aacccggaac gcaacagagc
     4621 ttcgtgggca tcccgatccg ggtcgtagca gcagtcctgg accaaacgca cgtcgtagtc
     4681 cgcatcacta gcccaggcga cgcttgaaag aacaacgccg gtggtgctta tcccggccat
     4741 gacaagcgtg cttacgccgc gcgtccgaag gtcggcgtcg agcgccgtgc cataaaagac
     4801 gctggctcgc ggacatgcat agaaaaggtc gccccgttcg atcgcaagcc cttcgacggg
     4861 cagaccggtg cgaaatcgtc cgctcgggag atacggtgag atttggcgat tcgtcgccgg
     4921 cggcgcatgt tcatactctt cgccgagcga aaagttgggg aacagcacgg gccggccgcg
     4981 caaggcgtgc tggcagccgt gcagaccctg cgtgagatga acgccgacaa cctgcgcaag
     5041 gtgccggccg atgcacccac ggccttcatc aagccgcgct ggaagccgct ggtgatcacc
     5101 ccggaaggcc tcgaccggaa attctacgaa atctgcgccc tgtccgagct gaagaacgcc
     5161 ctgcgctccg gcgacatctg ggtcaagggc tcgcggcagt tccgcgactt cgacgactac
     5221 ctgctgccgg ccgagaagtt cgccgcactc aagcgcgagc aggccctgcc cctggcgatc
     5281 aacccgaaca gcgaccagta cctggaagag cgtttgcagc tgctggacga gcagttggcc
     5341 accgtcaccc gcctggccaa ggacaacgag ctgcccgatg ccatcctcac cgagtcaggg
     5401 ctgaaaaatc accccgctgg atgcggcggt gccggatcga gcgcacgctg ttcatcctgg
     5461 actggctgca aagtgttgaa ctgcgccgcc gcgtgcatgc cggcctgaac aaaggtgagg
     5521 cgcgcaactc gctggccagg gcggtgttct tcaaccgcct tggggaaatc agggatcgga
     5581 gcttcgagca gcagcgctac cgggccagcg gcctcaacct ggtgacggcg gctatcgtgc
     5641 tgtggaacac ggtgtacctg gaacgcgcca cccaggggtt ggtcgaggcc ggcaagccgg
     5701 tggacggcga gctgctgcaa ttcctgtcgc cgctgggctg ggagcacatc aacctaaccg
     5761 gcgattacgt ctggcggcag agccgcagac tggaagacgg gaagtttcgg cccttacgga
     5821 tgcccggaaa accttagcgt acgatttttt ccgaattctg cgggctcccc tatgtgggcg
     5881 tttatgtaac atatgtcgct gggccaactc ttctggtttc tgggttttta tcgtggatta
     5941 caaggccgta atctgataat gcccctaatg ggtcagatct ctaggatagt agcaatacag
     6001 caaggaactt cttgagatcc tttctgttgc gtcgtaatct catgtcctgc aaacaaaaaa
     6061 accaccgcta ccaacggtgg ttcgtttgcc ggaatcaaga gctaccaact cttttttccg
     6121 aaggtaactg gctttcacgg agcgcagata ccaaaactgt ccttctagtg tagccgcagt
     6181 caggccacca cttcaagaac tctgtagaag cactctacta ctctgctccg tgaatcctgt
     6241 taccagtggc tgctgccagt ggcgcatttg catgtctttc cgggttggac tcaagacgat
     6301 agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct
     6361 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat
     6421 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcca
     6481 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag
     6541 cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgc cccgctgggg
     6601 cgtggccttt tcgcccttgt ccttagcttt cgttatgctt accgttacgt tatctgctta
     6661 tcttccctcc gctcgccgca gactcatgac cgaacggagt gagcgaatga gcgaggaagc
     6721 ggaaaaccat cagaacgtac agtaagcact ttcgcgccgg ggccttcatg aacaaacaac
     6781 aaaagacagc cgttaacatg gctaaattca tccaggatca gtccctgctt cttctggaca
     6841 ggcttaatga gctggatctg gatcatgagg ctgatttctg cgagaaactc catgaggatg
     6901 cggaacgcct ttaccgaagc ctgtcagcgc gcctgaatga gcagcaggac ggtgcgtaat
     6961 ggctccgttg tgggtattac cggagtaata ccggcatcgc cttaattttc attgtcttgg
     7021 tgcttaatat tattaccccg gtaataccca gaatcccttt attttttctt ttttatttat
     7081 ttttcatgaa gttgcgatga aaatcgcagc tgcgttaggt gtatgtcatt ttttagtata
     7141 taaaacgacc actcccgagt gctggcgccg caggttaagg atgattctgg ctaaaaagcg
     7201 tccttttccg ggaaagggtc atgctcgtat gaccctatgg gttcagcgac tacacataaa
     7261 ctacaaaatt gcatctgctt ttaccccaga acgcgggtgt acaacagaat tacatcaaaa
     7321 gtacatcaaa attacatcac ttttacgtca gatcgcgggt gtacaacaga attacatcaa
     7381 aattacatca cttttacgtc acttttacat catttttacg tcagatctga cctctgaact
     7441 attcgtacat ccgcccctct tcctgggcct cctgcacgtg ctgaaaatgg tttttccggc
     7501 tgtgccggca acgcctcacg gccacgcaat aaattgcgca tccgttcacc gttatcgggg
     7561 tggttcatgg tggctttcag ggcgttcatc ggattttagc cgctaaaatc ccggccaggt
     7621 atgtccgtgc atagccatgc agtcgcgcca gaatcatcag gggcgttttt tctcgatatg
     7681 ggcacagaaa ggtgtgtgtt tcaggccgga ggcctcagaa acgccctgag ggcctctgag
     7741 gcggtgcggg caacggatgt tatggtaaat ccggtggctt tgacgtgcct gccgttgtgt
     7801 accgtcgtga gtgaaaataa ttatattgtt cgaagaacag cgctttttgg ccggagaaaa
     7861 gcagttctgc aaacgatggt atatcgatat gagttctttt ggcattaatc caggcaagac
     7921 gccgggcaat aagcgtaata ttccggatgt tacttatttc catttctgtc aacagcatcc
     7981 ccccccacgc cggagaataa aaaagccgcc tgttccatcg gcaagcccta catcaaggga
     8041 aactacgtct tacgattcgt tttgaaccgg gacgctttgt tgctgcgaat cactaaccag
     8101 ataatccacg ttttgtgagt gggtttaggt taaggcttca atagaacaat aatagcgtta
     8161 agtgattagg gcaacctgtg attgctaaaa tgatgaagtc ttcacatcat ttcggtggta
     8221 atgactccaa cttactgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc
     8281 agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc
     8341 agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt
     8401 cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag
     8461 ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc
     8521 gcaacaaccg tcttccggag cctgtcatac gcgtaaaaca gccagcgctg gcgcgattta
     8581 gccccgacgt atccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc
     8641 tgtatgcgcg aggttaccga ctgcggcctg agttttttaa atggcggaaa atcgtgttga
     8701 ggccaacgcc cataatgcgg gcggttgccc ggcatccaac gccattcatg gccatatcaa
     8761 tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt
     8821 tacggcagtg agagcagaga tagcgctgat gtccggcagt gcttttgccg ttacgcacca
     8881 ccccgtcagt agctgaacag gagggacagc tgatagaaac agaagccact ggagcacctc
     8941 aaaaacacca tcatacacta aatcagtaag ttggcatcat caccccgctc atgagacaat
     9001 aaccctggta aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacattttc
     9061 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     9121 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     9181 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9241 tgagcacttt taaagttctg ctatgtggtg cggtattatc ccgtgttgac gccgggcaag
     9301 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9361 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9421 tgagtgataa cactgctgcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9481 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9541 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     9601 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9661 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9721 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     9781 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9841 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9901 aa
//
