LOCUS       NC_018996               5153 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli plasmid pHUSEC41-4, complete sequence.
ACCESSION   NC_018996
VERSION     NC_018996.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14229380
KEYWORDS    RefSeq; complete replicon.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Kunne,C., Billion,A., Mshana,S.E., Schmiedel,J., Domann,E.,
            Hossain,H., Hain,T., Imirzalioglu,C. and Chakraborty,T.
  TITLE     Complete sequences of plasmids from the hemolytic-uremic
            syndrome-associated Escherichia coli strain HUSEC41
  JOURNAL   J Bacteriol 194 (2), 532-533 (2012)
   PUBMED   22207742
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Kuenne,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2011) to the INSDC. Kuenne C., Medical
            Microbiology, Jusuts-Liebig University, Frankfurterstr. 107, Hessen,
            D-35392, GERMANY
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009955615.2-RS_2024_06_24
            Annotation Date              :: 06/24/2024 03:07:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 7
            CDSs (total)                 :: 7
            Genes (coding)               :: 7
            CDSs (with protein)          :: 7
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            HE603113.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="HUSEC41"
                     /serovar="O104:H4"
                     /db_xref="taxon:562"
                     /plasmid="pHUSEC41-4"
     gene            1..1566
                     /locus_tag="HUS41_RS00005"
                     /old_locus_tag="HUS41_pIV0001"
     CDS             1..1566
                     /locus_tag="HUS41_RS00005"
                     /old_locus_tag="HUS41_pIV0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000447003.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            1952..2089
                     /locus_tag="HUS41_RS00015"
                     /old_locus_tag="HUS41_pIV0003"
     CDS             1952..2089
                     /locus_tag="HUS41_RS00015"
                     /old_locus_tag="HUS41_pIV0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001329514.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            2648..2803
                     /locus_tag="HUS41_RS00020"
     CDS             2648..2803
                     /locus_tag="HUS41_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001490772.1"
                     /translation="MSVDFCDARQGGGAYGKTPAALASSGALLFAHMFFPALSPDSVDN
                     RITAFE"
                     /name="hypothetical protein"
     gene            complement(2931..3125)
                     /locus_tag="HUS41_RS00025"
                     /old_locus_tag="HUS41_pIV0005"
     CDS             complement(2931..3125)
                     /locus_tag="HUS41_RS00025"
                     /old_locus_tag="HUS41_pIV0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041621.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3409..3732
                     /gene="mobA"
                     /locus_tag="HUS41_RS00030"
                     /old_locus_tag="HUS41_pIV0006"
     CDS             3409..3732
                     /gene="mobA"
                     /locus_tag="HUS41_RS00030"
                     /old_locus_tag="HUS41_pIV0006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(3957..4376)
                     /locus_tag="HUS41_RS00035"
                     /old_locus_tag="HUS41_pIV0008"
     CDS             complement(3957..4376)
                     /locus_tag="HUS41_RS00035"
                     /old_locus_tag="HUS41_pIV0008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4457..4894)
                     /locus_tag="HUS41_RS00040"
                     /old_locus_tag="HUS41_pIV0009"
     CDS             complement(4457..4894)
                     /locus_tag="HUS41_RS00040"
                     /old_locus_tag="HUS41_pIV0009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001318234.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     Typing          3161..3400
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          complement(2373..2902)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.59"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          3410..3729
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.11e-62"
ORIGIN
        1 atggaaacag ctgtagcgta ctataaagat ggtgttcctt atgatgataa ggggcaggta
       61 atcattactc ttttgaatgg taatccagac gggagtggct ctggcggtgg tggtggaact
      121 ggaggtagca aaagtgaaag ttctgcagcc attcatgcca cagctaaatg gtctactgct
      181 caattgaaga aaacgcaggc agaacaggct gcccgagcaa aagctgccgc agaagcacag
      241 gctaaagcaa aagcaaaccg ggatgcgctg actcaacatc tgaaggatat tgtgaatgag
      301 gcgcttcgcc ataattccac tcatccggag gttattgacc tggctcatgc caataatgca
      361 gcgatgcagg cagaagcaga gcggttgcgc cttgcaaaag cagaagaaaa agcccgtaaa
      421 gaagcggaag ctgcggaaaa ggcttttcag gaagcagaac aacgacgtaa agagatagag
      481 aaggagcagg ctgaaacaga acgccaattg aaactggctg aggatgaaga gaaacgcctg
      541 gcagcattga gtgaagaggc ccgggctgtg gaggtggcac aaaaaaatct tgctgctgca
      601 caatctgagc tggcgaaagt ggatgaagag attaatacgc tcaatacccg tttaagctcc
      661 agtattcatg cccgtgatgc agaaacgaat acgctgtccg gaaaacgaaa tgagttggat
      721 caggcatctg ctaaatataa agaactggat gaaagggtta aacttttatc tcctagggcg
      781 aatgatccgc ttcagagtcg tccttttttt gaggccacca gactacgggc gagagctggt
      841 gatgagatgg aggaaaaaca aaagcaggta acagcatcag aaacgcgtct taaccagatt
      901 agctctgaga taaatggaat ccaagaggct atttctcagg ctaataataa gcgaagtaca
      961 gcagtttcac gtattcatga tgctgaagat aatttgaaaa cagcacagac taatctcctg
     1021 aactcgcaga ttaaggatgc tgtggatgca acagttagct tttatcaaac gctatctgaa
     1081 aaatatggtg aaaaatattc aaaaatggca caggaacttg ctgataaatc taaaggtaag
     1141 aaaatcagca atgtgaatga agctctagct gcttttgaga aatacaagga tgtattaaat
     1201 aagaaattca gcaaagcaga ccgtgatgca attttcaatg cactggaggc ggttaagtat
     1261 gaagactggg ctaagcattt agatcagttt gccaagtact tgaagattac gggacatgtt
     1321 tcttttggat atgatgtggt atctgatatc ctaaaaatta aggatacagg tgactggaag
     1381 ccgctatttc ttacattaga gaagaaagct gtagatgctg gagttagtta tgttgttgtt
     1441 ttacttttta gtgtgcttgc tggaactaca ttaggtatct gggggattgc tattgttaca
     1501 ggcattctat gtgcctttat tgataagaat aaacttaata ctataaatga ggtgttgggt
     1561 atttaattat tctttattga tataaacagt cctagcaatc caaatgggat tgctaggacc
     1621 aaacaaagta gattgtatag cataaatagg tttaattttg ccacgggggc attatttagg
     1681 ttttttcttt ttctgaaaaa atctttcttt atgaacttaa aaaatatgtg ttcaatagca
     1741 tattttgaat atgggcatag aatagtgctt atcactattg cgtatagcat cttatctgac
     1801 gctaggaaat aatacccttc gttgtttttt gttataaggt atatatatat aagtgcgcag
     1861 tataggccaa acaaaatatt ttttatgtag tatcttaaac tcataaatta aacctcgtta
     1921 tatattcttt tcattttata aggatcgagt tatgaggaaa agattttttg tgggaatatt
     1981 cgctataaac ctccttgttg gatgtcaggc taactatata cgtgatgttc agggagggac
     2041 tgtggctcca tcatcttctt ctaaattgac ggggatctct gttcagtaga aaagatcaaa
     2101 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcctgtaaac gaaaaaaccg
     2161 cctggggagg cggtttgatc gaaggttaag tcagttgggg aactgcttaa ccgaggtaac
     2221 tggcttcagc agagcgcaga taccaaatac tgtccttcca gtgtagccgt agtcgggcca
     2281 ctacttcaag aactctgttg caccatttgc gccatcatcg ctctgctaac caagttacca
     2341 acggctgctg ccagtggcgc taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2401 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2461 cgaacgacct acaccgagcc gagataccaa cagcgtgagc tatgagaaag cgccacgctt
     2521 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2581 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2641 ctctggcttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2701 gcctgcggcg ctggcttctt ccggtgcttt gctttttgct cacatgttct ttccggcttt
     2761 atcccctgat tctgtggata accgtattac cgcttttgag tgagctgaca ccgctcgccg
     2821 cagtcgaacg tccgagcgta gcgagtcagt gagcgaggaa gcggaagagc gccttatgtg
     2881 acattttctc cttacgctct gttgtgccgt tcggcatcct gtcctgagcg ttatctctct
     2941 gtgctatttt ctacttcaaa gcgtgtctgg atgctgttct ggagttcttc tgcgagttcg
     3001 tgcagcttct cacacatggt agcctgttcg tcagcatcga gtgcgtccag cttttcgagc
     3061 agcgtcaggc tttggctttt tatgaatcct gccatgttga gtacggcttg ctgctgcttg
     3121 ttcatctttt cgttttctcc gttctgtctg tcatctgcgt tgtgtgatta tatcgcgtac
     3181 cacttttcga ctgttttacc gccggtattc tgccgtctga cgctttgacg ggtatttctg
     3241 cctgacaata ctgtcactgc caaaaaactg ccgtgccttt gtcggtaatt cgaacttgct
     3301 gacaggacag gatgtgtaat tgttataccg cgcatacatg cacgctatta caattaccct
     3361 ggtcagggct ttgccccgac acccatgtca gatacggagc catgttttat gacaaaacga
     3421 agtggaagta atacgcgcag gcgggctatc agtcgccctg ttcgtctgac ggcagaagaa
     3481 gaccaggaaa tcagaaaaag ggctgctgaa tgcggcaaga ccgtttccgg ttttttacgg
     3541 gcggcagctc tcggtaagaa agttaactca ctgactgatg atcgggtact gaaagaagtt
     3601 atgagactgg gggcgttaca gaaaaaactc tttatcgacg gcaagcgtgt cggggacagg
     3661 gagtatgcgg aggtgctgat cgctattacg gagtatcacc gtgccctgtt atccaggctt
     3721 atggcagatt agcttcccgg agagaaaact gtcgaaaact gacggtatga acaccgtaag
     3781 ctcccaaagt gatcaccatt cgctttcatg catagctatg cagagagctg aaaacgatcc
     3841 tgacgcatcc ttcctgtttt tccggggtaa aacatctctt tttgcggtgt ctgcgtcaga
     3901 atcgcgttca gcgcgtttca gtggtgcgta caattaaggg attatggtaa atccacttac
     3961 tgtctgccct cgtagccatc gagataaacc gcagaaatcg tgtcagccag cagcctggat
     4021 tttttcgggg taaggtcttt gctgcggtaa tcagtacccg caaattgagt tgtgccccgg
     4081 agtgagttta aaaattcctg gctgtgaaac tgttgaatgc gttgttgcac atcactccgt
     4141 gattttcctg tcgctttatc tttagctccc atatcccaga actgccgaaa aaaaggcacc
     4201 atcattcgtg ttgtgtcggc tttttgggtg gaaaagttgg gatcaaaacc gtcatcagaa
     4261 gcatatacgt aatgccgggt atgttgttcc acgctgccct ttggggtaca ggctgtgaga
     4321 gctactgttg ctgttatcat ggcgataaga caaagtttgt ttccggtacg tctcatgagt
     4381 gtctccctac cttaaagtat ttattctcgg tgagttatta tccgaagatt atcttatcat
     4441 cagtttctga gcgcgttcag tgaccgaaca gcctggacca gaatccccgt ttcttttcct
     4501 gctccagagc ctgcctgagc tgggctatct cattctgtag ctgtttccgt tctgcttcct
     4561 ggcgccggcg ctccgtatcc tgggcttgtt tatcctcaag catcagcgtc aggcattgtt
     4621 tcagctcatt cagttcccgg agaatgcgtt ctgtctgctg atcatgaggt ttttctgagt
     4681 gtccttgagt gtgcctttct ggtgtctcat tccgctttaa ttcgccgtat gcccggatca
     4741 gttcactggt ttcaaactcc cgtcgtccgt cctggctggt acggtaactg acaagacctg
     4801 agcgcatatc acggtaaaac tgtgaacgcg atcttccagt cattttcagt gcgtcccgga
     4861 gtgtgtgcca tgccataaag ttacagtgtc ccataggtgt ctcatctcat ggtttcagta
     4921 aaacataatg aggtctgaga acggtaatgt ttgtgctggt ttttgtggca tcgggtggaa
     4981 atagcacgtg gtgtgggtgg ctgttttttt gatcgttttc acaaaaatga aaatccacag
     5041 ggttgacagg gaaaatgcag cggcgtaatt ttaaactgta tataaaacca gtgattatat
     5101 atacagtata ttgcttttaa ctatttgttt ttaatgtcaa agaggatttt ata
//
