LOCUS       NC_018884               5518 bp    DNA     circular CON 14-DEC-2023
DEFINITION  Bacillus thuringiensis Bt407 plasmid BTB_5p, complete sequence.
ACCESSION   NC_018884
VERSION     NC_018884.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02603279
            Assembly: GCF_000306745.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis Bt407
  ORGANISM  Bacillus thuringiensis Bt407
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Sheppard,A.E., Poehlein,A., Rosenstiel,P., Liesegang,H. and
            Schulenburg,H.
  TITLE     Complete Genome Sequence of Bacillus thuringiensis Strain 407 Cry-
  JOURNAL   Genome Announc 1 (1) (2013)
   PUBMED   23405326
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Sheppard,A.E., Poehlein,A., Liesegang,H., Rosenstiel,P. and
            Schulenburg,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2012) Department of Genomic and Applied
            Microbiology, Goettingen Genomics Laboratory,
            Georg-August-University-Goettingen, Grisebachstr. 8, Goettingen,
            Lower Saxony 37079, Germany
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Newbler v. v2.6
            Sequencing Technology             :: Sanger dideoxy sequencing; 454
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/14/2023 02:22:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,598
            CDSs (total)                      :: 6,409
            Genes (coding)                    :: 6,240
            CDSs (with protein)               :: 6,240
            Genes (RNA)                       :: 189
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 142
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 95 of 169
            Pseudo Genes (incomplete)         :: 72 of 169
            Pseudo Genes (internal stop)      :: 33 of 169
            Pseudo Genes (multiple problems)  :: 26 of 169
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003896.1.
            Source DNA available from Hinrich Schulenburg, Evolutionary Ecology
            Genetics Zoological Institute, CAU Kiel, Am Botanischen Garten 1-9
            24118 Kiel, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Bacillus thuringiensis Bt407"
                     /mol_type="genomic DNA"
                     /strain="407"
                     /db_xref="taxon:527021"
                     /plasmid="BTB_5p"
     gene            101..1438
                     /gene="mobV"
                     /locus_tag="BTB_RS32200"
                     /old_locus_tag="BTB_5p00010"
     CDS             101..1438
                     /gene="mobV"
                     /locus_tag="BTB_RS32200"
                     /old_locus_tag="BTB_5p00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031674.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_001031677.1"
                     /translation="MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNY
                     DLLNQQKIRYESTIKQEISERVKRKPRANSVVLSEFVVTASPDYMHSLSLEEQKRYFES
                     SLDFIQKRYGKQNTLYAMVHMDEATPHMHIGVMPITEDNRLSAKDMFTRKELISLQQDF
                     PLEMREKGFDVDRGEGSEKKHLSPQAFKEKQDLEVEVEQLSNVKTHLKTKVVETHNQLQ
                     QTTNYIEKQNETLQKIQQQFLSLDKKIKEKKQEFETFRNQIPDKPVSMSYLREETKTEV
                     TTKLFGKPEITEKKTGNIVVTREQWRDMTEKVNAAVIVKKDYERLQKTDLVKENQSLRE
                     DNKYLEETIKGNNLALKHSYKQNRELEEVNKELHTEIGTLKAHIRDLQMNIKVLYQQTK
                     KVFKEQFKAFRGLIKNELDMKGVDNQFEREHTREIRSRQKGYDMER"
                     /name="mobV"
     gene            2649..3263
                     /locus_tag="BTB_RS32205"
                     /old_locus_tag="BTB_5p00020"
     CDS             2649..3263
                     /locus_tag="BTB_RS32205"
                     /old_locus_tag="BTB_5p00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185569.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000185569.1"
                     /translation="MTNTIDFKHVEKNARIRDFENEKEKFKQDHNGINQEEVNQAMQVL
                     SKATGGKEIFIGTKRSPQSKVKFAQFIQDNWDYALENAFFTDEEMLFLLRIQRFLQFKS
                     NCIVNDIHSRNALPMSQKQIADRLKTDKSKISRIVNSLVQKGVIVKANGHKPEGVKART
                     YALFINPNIIYSGERDNVETTLKALFMNSKSLFKKFPIALF"
                     /name="helix-turn-helix domain-containing protein"
     gene            3827..4639
                     /locus_tag="BTB_RS32210"
                     /old_locus_tag="BTB_5p00030"
     CDS             3827..4639
                     /locus_tag="BTB_RS32210"
                     /old_locus_tag="BTB_5p00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000734781.1"
                     /translation="MKKLLISFIAILFFICGFNLKAFAAEEIIDYQSLYNQAIQEGVLD
                     QNSVSYNEWLKQNKEEFMPIYQDGLKQGVFLEPLSYNEWLKLNNYGQAPTGDIELFDDV
                     TPRGSWGGFTLKAGDIFITNATSSAGIVGHAAIANGDNYILHMPGAGQGNQQLSTSNWM
                     QKYTASGKWIKVYRLKDQTLARDVARYADRNFYSTTGSATKNVYLDYGIDTHLYQKNPT
                     YCSKLVFQALYFGSGSRNVMQAVSGIVTPYGLIDTFTSAYRPSLVKTY"
                     /name="hypothetical protein"
     gene            4686..5030
                     /locus_tag="BTB_RS32215"
                     /old_locus_tag="BTB_5p00040"
     CDS             4686..5030
                     /locus_tag="BTB_RS32215"
                     /old_locus_tag="BTB_5p00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="WP_000968001.1"
                     /translation="MMAIFISIVLVVSFVLFNRVQAKKSIYNYIARQGIQESQLKYIDF
                     HKDFKMGGYWLAVYVEGENPDIYYEYSYQDKKVNFQAYFNSEKAIKKKMWGGSGLTEIE
                     MKKLKYPPLQ"
                     /name="DUF3139 domain-containing protein"
     Typing          102..1435
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtataatgg gctataccaa aactttggat tcgctctctc gatatgttat gataagctca
       61 aaagtagtcc tcttacggaa gacaattgtg agggaaaaac atgaacaaat ttgcaatcat
      121 tcacatgcag aaatttcaaa tttctgatgt gcaagggatt caaaaacata atcaaagaca
      181 aggaaaaagt aaatcaaatt tagacatcga ttattcgaaa agtgaacaga attatgattt
      241 gttgaatcaa caaaaaatta gatatgaaag cacaattaaa caagagatca gtgagcgagt
      301 aaaacgaaag ccacgggcaa attctgttgt gctttcagaa tttgtagtaa cagcttcgcc
      361 tgattacatg cattcattaa gtttagaaga acagaaacgt tattttgaga gttcactaga
      421 ttttattcaa aagcgttatg gcaaacaaaa tacgttgtat gcgatggttc atatggatga
      481 agcgacacca catatgcata ttggtgtgat gccaattaca gaagacaatc gtttgtccgc
      541 aaaagatatg tttacgagaa aagaattgat atctttgcaa caggacttcc cgttggaaat
      601 gcgggaaaaa ggttttgatg tagatcgggg agaaggttct gagaaaaaac atttatcgcc
      661 tcaagccttt aaggaaaaac aagatttaga agtagaagtg gaacagttat ccaatgtaaa
      721 aacgcattta aagacaaaag tagtggaaac gcataatcaa ttacaacaaa ccacaaacta
      781 catcgaaaaa cagaatgaaa cgttacaaaa aattcagcaa caatttttga gtttagataa
      841 aaaaataaaa gaaaagaaac aggaatttga aacgtttcga aatcaaattc cggataagcc
      901 agtgtcaatg tcttatttgc gagaagaaac aaaaacagaa gtaacaacga aactgtttgg
      961 aaagccagaa attactgaga agaaaacagg aaacattgtg gttacacgtg agcaatggag
     1021 agatatgaca gaaaaagtga atgcagccgt tattgttaaa aaagattatg aacgtttaca
     1081 gaagacggat cttgtaaaag agaatcaaag tttacgagaa gataataaat atttagaaga
     1141 aacaataaag gggaataatc ttgctttaaa acattcttat aaacaaaatc gggaattaga
     1201 agaagtaaat aaagaattgc atactgaaat aggcacttta aaggctcata taagagattt
     1261 acagatgaat ataaaggttt tatatcaaca gacgaaaaaa gtctttaaag agcaatttaa
     1321 ggcatttaga ggacttatta aaaacgaact ggatatgaag ggtgtagata atcaatttga
     1381 acgtgaacat acaagagaaa taagaagtag acagaaagga tatgacatgg aacgttagaa
     1441 acgttttgtg atatactata attattcatc actaactaat ttatgggttt catccattta
     1501 aaaatctctt tatatgtgtg catataaaga gattttttgc gtttatgtaa ttaaaattat
     1561 gttaagattg ataagactac atttgaaaat gtgggttcaa gttgctgttt gactgaattg
     1621 cacaaaaaac caattctatt acacgagaat tggttttttt gcgtataacg aatagagata
     1681 tatctaggcg tttaaggagt tgtatcttat ataatttagt ctaacattaa taaaaagaaa
     1741 attcaaaaat attcactagg tttgttgttt ataaggaatt ataaaacatg aaattttgca
     1801 tattttctaa ttaattcccg gaaataggaa ttaatgattt ataataaata gatgaaatac
     1861 cctttcagtt tccgatgaac tgtactttag tctgttgtta gtacgaagtc ttttaaaaga
     1921 cttcgtactt tttttattgt cgaccaagac tatgttgtaa atgctcatag cattgtatgt
     1981 tggatttcaa ttgtaagttc caaagtaaac ttttgaatat ttgtttactt tcaaataatt
     2041 ggttttatgg tgttttcaga ggattttaga aaaatagaga ttcccgtaaa gaaagtatac
     2101 atttagaatg cgaattttgt acggatgatg gttaaagata ctcaataaaa atttcatttt
     2161 gtagaaaatc atcaaaataa aacgagcact tatggcaagg cgctcgttta aaaaacattc
     2221 tgtgtattag ggttgtgttt ataagttatg aaccgatgaa tatttgttaa agtaaggttt
     2281 aagatagttt attcatttta aagtaaatgg gaacctgtac ggcaaaaaaa gaggaccttc
     2341 gaacagtgct ctttctctga gtctgtagtg catgatactt tatgaagttt ttttagaaat
     2401 aaaatgaatg agctcacgac taaaacgaac ttgctataca ataacatata aaaagtcaga
     2461 caagatacaa caaaaaaagc cattcatgct ataattgagt tgtcgaatct acaactatag
     2521 gagaagggct ttttttactg ttatacaatt aattagagtg ttcagactca aattaattta
     2581 tctaacttca taataacaaa ataaaagtct cttcgctagt tgtaaatact agaaacgagg
     2641 agaaaagcat gacaaatacc attgatttta aacatgttga gaaaaatgca agaatacgtg
     2701 attttgagaa tgaaaaagaa aagtttaaac aagatcataa cggaattaat caagaagaag
     2761 taaatcaagc aatgcaagta ttaagtaaag ctactggcgg aaaagaaata tttataggta
     2821 ctaaacgttc cccgcaatca aaagtaaagt ttgcccaatt tattcaagat aattgggatt
     2881 atgctttaga aaatgcattt tttactgacg aagaaatgct ctttttactt cgtattcaaa
     2941 ggtttttaca atttaaaagt aattgtattg taaatgatat tcattctaga aatgcacttc
     3001 ctatgagtca aaaacaaata gctgatagat taaaaactga taaatctaaa attagtcgca
     3061 ttgtaaatag tctggtacaa aaaggtgtta ttgtaaaagc aaatggacat aaaccagaag
     3121 gggttaaagc tcgtacatac gcattattta ttaatcctaa tattatttat agcggtgaac
     3181 gtgataatgt tgaaacaact ttaaaagctt tatttatgaa ttcgaaatca ctatttaaaa
     3241 aatttccaat agcccttttt taagggctat ttttttaatt ttaatagttg tctaatttga
     3301 caactaaagt tgtctaatct gacaactatt aaaaccacta taaacccttg atataataag
     3361 ctttttaact agtttagtag gtattttact ctcactctct atataagggc taaaagttcc
     3421 gcaaggaaca ggtcgtattt ttgaggaaaa agcactcaaa aatctccacc tttttcacta
     3481 cgtttcatcc ttgccaaact tttttcggct ggtttttagc tttcgccaag ccgaacagac
     3541 caaaaacggg gttaagttgc tcgacttggg ctagcaattg taccagccga accgccaatt
     3601 cttccgttca aagcatgatt ctttttccat atgtatcaac gctatgtggg gtttagcccc
     3661 cacacgacta gccagcactc acacccaaaa acaagggaga attacattat ttagctaata
     3721 aatatttgta tgttttttgt cacaagtatt tacattttga atttgaaaat cgtgttacct
     3781 ttataaaagg ttaccgaaat ataataatta tttagggaga caatttatga aaaaattatt
     3841 aattagtttt attgctattt tattttttat ttgtggtttt aacttgaaag cgtttgctgc
     3901 tgaggaaatt attgattatc aatctcttta taatcaagca attcaagaag gtgttttaga
     3961 tcaaaatagt gtatcttata atgaatggtt aaaacaaaat aaagaagaat ttatgcctat
     4021 atatcaagat ggtttaaaac aaggtgtttt tctagaaccg ttatcttata atgaatggtt
     4081 aaaattaaat aattatggac aagcaccaac gggagatatt gaactttttg atgacgtgac
     4141 tccaagagga agttggggtg gatttacttt aaaagctgga gatatattta taacaaacgc
     4201 aactagttca gcaggtatag taggacatgc agctattgct aatggtgata attatatttt
     4261 acatatgcct ggtgctggcc aaggaaatca acaattatct acatctaatt ggatgcaaaa
     4321 atatactgcg tcaggaaaat ggattaaggt ttatcgatta aaagatcaaa cacttgctag
     4381 agatgtagct agatatgctg atagaaattt ttattccact actggaagtg caactaaaaa
     4441 tgtttattta gattatggta tcgatacaca tctgtatcaa aaaaatccaa cttattgttc
     4501 taaattagta tttcaagcac tctattttgg ttctggttca agaaatgtta tgcaagcggt
     4561 tagtggaatc gtaactcctt atggtttaat tgatactttt acttcagcat atagaccgtc
     4621 attagtaaaa acatattaat aaataattta ggaggattga atttgaaaaa aaaaagatgt
     4681 taataatgat ggctatcttt atatcaatag ttttagtggt tagttttgtt ttatttaata
     4741 gagtacaggc gaaaaaatct atatataatt acattgctag acaaggaata caagaaagtc
     4801 agttgaaata tattgatttt cataaagact ttaaaatggg cggatattgg cttgctgttt
     4861 atgtggaagg agaaaatcct gatatttatt atgaatattc ttatcaagat aaaaaagtta
     4921 attttcaagc ttactttaat tctgaaaaag cgataaagaa aaaaatgtgg ggtggaagtg
     4981 gacttactga aatagagatg aaaaaattaa agtatccacc tttgcaataa taaacagtat
     5041 aaaaaagaac ttaaatacag gataaaacaa agactaatta gtactaaata gataagtact
     5101 aattagtctt tttatttttt aattaattta gaattaagag tttgataaag gatttaatga
     5161 atgattgaaa taactcgcaa aacgactatt gggagaagaa aaataagcgg taagtgtcaa
     5221 tggtaagcgg actggaatgg ttcagcggtt tatgctgatc catgaaagga gcattaagga
     5281 atggacacgt gcaaccgatt ttccatcctc ttggtagttc ctcaaaacga accgttttgg
     5341 ggacgccaac cggcgaggaa gcccctcaag gattgagggg ttggggagtt ggatcgatgg
     5401 ggtcaagggg aagagttccc cttgtggggt gtggggcaaa gcccctggtc tttatcacgc
     5461 tgtggcgtga tttggcgaag ccaacgaggt ctcgcagagc ccacaccaaa tgtaattt
//
