LOCUS       NC_018883               6880 bp    DNA     circular CON 14-DEC-2023
DEFINITION  Bacillus thuringiensis Bt407 plasmid BTB_6p, complete sequence.
ACCESSION   NC_018883
VERSION     NC_018883.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02603279
            Assembly: GCF_000306745.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis Bt407
  ORGANISM  Bacillus thuringiensis Bt407
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Sheppard,A.E., Poehlein,A., Rosenstiel,P., Liesegang,H. and
            Schulenburg,H.
  TITLE     Complete Genome Sequence of Bacillus thuringiensis Strain 407 Cry-
  JOURNAL   Genome Announc 1 (1) (2013)
   PUBMED   23405326
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Sheppard,A.E., Poehlein,A., Liesegang,H., Rosenstiel,P. and
            Schulenburg,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2012) Department of Genomic and Applied
            Microbiology, Goettingen Genomics Laboratory,
            Georg-August-University-Goettingen, Grisebachstr. 8, Goettingen,
            Lower Saxony 37079, Germany
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Newbler v. v2.6
            Sequencing Technology             :: Sanger dideoxy sequencing; 454
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/14/2023 02:22:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,598
            CDSs (total)                      :: 6,409
            Genes (coding)                    :: 6,240
            CDSs (with protein)               :: 6,240
            Genes (RNA)                       :: 189
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 142
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 95 of 169
            Pseudo Genes (incomplete)         :: 72 of 169
            Pseudo Genes (internal stop)      :: 33 of 169
            Pseudo Genes (multiple problems)  :: 26 of 169
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003895.1.
            Source DNA available from Hinrich Schulenburg, Evolutionary Ecology
            Genetics Zoological Institute, CAU Kiel, Am Botanischen Garten 1-9
            24118 Kiel, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Bacillus thuringiensis Bt407"
                     /mol_type="genomic DNA"
                     /strain="407"
                     /db_xref="taxon:527021"
                     /plasmid="BTB_6p"
     gene            101..1417
                     /gene="mobV"
                     /locus_tag="BTB_RS32165"
                     /old_locus_tag="BTB_6p00010"
     CDS             101..1417
                     /gene="mobV"
                     /locus_tag="BTB_RS32165"
                     /old_locus_tag="BTB_6p00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099255.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_001031673.1"
                     /translation="MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNY
                     DLLNQQKIRYESTIKQEISERVKRKPRANSVVLSEFVVTASPDYMHSLRLEEQKRYFES
                     SLVFIQKRYGKQNTLYAMVHMDEATPHMHIGVMPITEDNRLSAKDMFTRKELISLQQDF
                     PLEMREKGFDVERGEGSEKKHLSPQAFKEKQDLEIEVKQLSNVKTHLKTKVVETHNQLQ
                     QTTNYIEKQNETLQKIQQQFLSLDKKIKEKKQEFEMFRNQIPDKSVSMSYLREETKTEV
                     TTKLFGKPEIIEKKTGNIVVTREQWRDMTEKVNAAVIIKSDYESLQKTDLVKENKQLHE
                     AVDGICDSLQDSQKRNLKLQEENKQLRTEISSLKAHIRDLQINIKVLYQQTKKVFKEQF
                     KAFRGLIKNELDIKDVDNQFEREHAREVKSRQKGYDMER"
                     /name="mobV"
     gene            2328..3071
                     /locus_tag="BTB_RS32170"
                     /old_locus_tag="BTB_6p00020"
     CDS             2328..3071
                     /locus_tag="BTB_RS32170"
                     /old_locus_tag="BTB_6p00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003308357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000782444.1"
                     /translation="MKMKKLGAGLLSAALLFPVVAPVVSHAEENTTVSEKNQIQETTKK
                     YFKSVEGMGTQYTEKQSKEIIDSVAIVSKYIKPNEHNQLELDSKVKEQVSPFVYEHYQR
                     GIQAINQATEAGYATINVENNKLEATPLANSSMNNLVGFNAKDGIQYSSVDSYWWGVKW
                     YMSKSESQQWQNNFADGSFYWGSIAAITGLLTPFFPPAAIASAVSVIMSVGNYHCYREL
                     RDHTSSRGSVMTISWLDKEIYANKR"
                     /name="hypothetical protein"
     gene            3098..3364
                     /locus_tag="BTB_RS32175"
     CDS             3098..3364
                     /locus_tag="BTB_RS32175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000144827.1"
                     /translation="MTFVMIQIGIAIFLLFIIGLIYKVIGKKFNSKFSMYFLVIIVFFA
                     LLFLVISEINTNGFTDLKVLVFLPLIVLSLILLIINAIKSKKI"
                     /name="hypothetical protein"
     gene            complement(3429..3569)
                     /locus_tag="BTB_RS35220"
     CDS             complement(3429..3569)
                     /locus_tag="BTB_RS35220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000751237.1"
                     /translation="MKKTILTLMGIITVFTLTLSNINTPKENKDPSIQKIMLMSDGNTG
                     G"
                     /name="hypothetical protein"
     gene            complement(3566..4660)
                     /locus_tag="BTB_RS32180"
                     /old_locus_tag="BTB_6p00030"
     CDS             complement(3566..4660)
                     /locus_tag="BTB_RS32180"
                     /old_locus_tag="BTB_6p00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000383146.1"
                     /translation="MDVQTKSNEQITNMLNDWYIEIRARHLGNAHKLRLEIDKKIHNIE
                     EDQNLLLYYSLLDFRHQYLIDHLSIGKDSFDKIESFNTPTDNLLSYYYFFFKAIHATSI
                     GNYNLAREYYDKAEIKLKEIPDQLEHAEFYYKLSTFSCHNQQYVPAIKQASKAKEIFSK
                     YKGYELNIGYCNNLSGLSCTHLKEYELAEEYFISAIDIFQKEKEEQAILYVRHNLGFMY
                     AKQNLSELAIRYLSEVIQKSPNNYRAILIEACEHAKLNNKNKALELLEKGLKISNNLKI
                     EEYQHRFKILLAINNEIPGEKLEQIILAGMIYFEKENLYEYIAEYNETLAIKFYHEDNH
                     SKASKYFYLSSKARKKSHNKGALK"
                     /name="hypothetical protein"
     gene            5114..5728
                     /locus_tag="BTB_RS32185"
                     /old_locus_tag="BTB_6p00040"
     CDS             5114..5728
                     /locus_tag="BTB_RS32185"
                     /old_locus_tag="BTB_6p00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133991.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001133991.1"
                     /translation="MPNTIEFKQVEKNARIRDLEIENEKFKQDHHEVSKDELEKAMETM
                     AKATGKELYIGTKKSPHSRVRFAQSLQENLGFLNKNGYLTNKEKVFLSDITPYIAFSSN
                     CIVHDIKAKNPVPANVSEIAKLIGISRQNTSLAINSLVKKGLLFKGDSGVEGNNAKAYA
                     VFVNPHIIYAGDKDSVNEALQVMFYKAMKMKILKDLPDKLF"
                     /name="helix-turn-helix domain-containing protein"
     gene            6272..6568
                     /locus_tag="BTB_RS32190"
     CDS             6272..6568
                     /locus_tag="BTB_RS32190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000043575.1"
                     /translation="MSKQQVKFYVMEKEMERLKLLSEMRYMTVPNYAKLTALGVQIRQP
                     KEIVVGNVELQEEDKAILKEILERRRGISYIAYDTGFNQRLEQLAKRLLGEEG"
                     /name="hypothetical protein"
     Typing          102..1414
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.112"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtatagtgg gctataccaa aactttggat tcgctctctc aatatgttat gatagtctca
       61 aaagtagtcc tcttacggaa gacaattgtg agggaaaaac atgaacaaat tcgcaatcat
      121 tcacatgcag aaatttcaaa tttctgatgt gcaaggaatt caaaaacata atcagagaca
      181 aggaaaaagt aaatccaatt tggatattga ttattcgaaa agtgaacaaa attatgattt
      241 gttgaatcag caaaaaatta gatatgaaag cacaattaaa caagagatca gtgaacgagt
      301 aaaacgaaag ccacgagcca attcggttgt actttcagaa tttgtagtaa cagcttcgcc
      361 tgattacatg cattcattac gtttagagga acagaaacgt tattttgaga gttcactagt
      421 gtttattcaa aaacgttatg ggaaacaaaa tacgttgtat gcgatggttc atatggatga
      481 agcgacacca catatgcata ttggtgtcat gccaattaca gaagacaatc gtttgtccgc
      541 aaaagatatg tttacgagaa aagagctcat atctttacaa caggacttcc cgttggaaat
      601 gcgggaaaaa ggttttgatg tagagcgggg agaaggttca gagaaaaaac atttatcgcc
      661 tcaagccttt aaggaaaaac aagatttaga aatagaagtg aaacaattat ccaatgtaaa
      721 aacgcattta aagacaaaag tagtggaaac gcataatcaa ttacaacaaa ccacaaacta
      781 catcgaaaaa cagaatgaaa cgttacaaaa aattcagcaa caatttttga gtttagataa
      841 aaaaataaaa gaaaagaaac aggaatttga aatgtttcga aaccaaattc cggataagtc
      901 agtgtcaatg tcttatttgc gagaagaaac aaaaacagaa gtaacaacaa aactgtttgg
      961 aaaaccagaa atcatagaga aaaaaacagg aaacatcgtg gttacacgtg agcaatggag
     1021 agatatgaca gaaaaagtga atgcagcggt tattataaag agcgattatg aaagtttgca
     1081 aaaaacggat cttgtaaaag aaaacaaaca attacatgag gcagtcgatg gaatatgcga
     1141 ttcacttcaa gattctcaaa agagaaattt aaagttacaa gaggaaaaca aacaattaag
     1201 aacagaaata agctcgttaa aggctcatat aagagacttg cagattaata taaaggtttt
     1261 atatcaacag acgaaaaaag tctttaaaga gcaatttaag gcgtttagag gacttattaa
     1321 gaacgaattg gatataaagg atgtagataa tcaatttgaa cgtgaacatg caagagaagt
     1381 aaaaagtaga cagaaaggat atgacatgga acgttagaaa aaggtatgtt gtaaaaaaga
     1441 gcataaggaa accgaatccc actttgtata aaaagtggtg aaataggctg tgcctattgg
     1501 gtttctaaag atttctagct ggtacgcttt ttctatgatc tgtggtcgtc tagtggtcgt
     1561 ttatagctgt tctgtggcct tccagtggcc tttggtggtc atttagtggc tgtttaaaat
     1621 gtactaaaaa tttaaatatg ctacaataaa tatccatcac aaataacttt ttaaaggtta
     1681 catctaaaaa atctctttgt atatttatat ataaagagat tttttacgtt tatgtaattg
     1741 aaattatgtt aaagttaaca agactacatt tgagaatgtg ggttcaagtt gctgtttgac
     1801 tgaattgcac aaaaaaacca attccattac acgggaattg gtttttttgg tgtctttggg
     1861 ttgtagattt attgacagcc taaaggagat tagtttcttt atagtctagt ataactaaaa
     1921 tagaaagaaa attcaaaaat aaaaggatga atgtttacat attttcaaat cagaacacaa
     1981 aaatgtaagg atttgggcta taatgagtag tgaaacactc tcctatgttt ctagtgattg
     2041 tatctgtttt tggtgcgaaa tctgtagata gattttgcac ttttttattt tttgttgaat
     2101 aggatcagtg tcatttaaat aagattgatt tcaatctatt aattttattg gattgtaaga
     2161 gtattttgag gaaatgtcga aatatattaa ttcgtattat tcttaaattt gtttattttc
     2221 tgaaaagtat aagttaatat aatgatgtac cagataatgg gataatttac aaattcagat
     2281 atgtaaatgt taattatttg gaaattaaat atgaagggat gaatattatg aaaatgaaaa
     2341 aattaggagc aggattactt tcagcggctt tattatttcc agtggtagca ccggttgttt
     2401 ctcatgcaga agaaaataca actgtatctg agaaaaatca aattcaagag acaacaaaaa
     2461 aatattttaa atctgtagaa gggatgggaa ctcaatatac tgaaaaacaa tctaaagaaa
     2521 ttattgattc tgtagctatt gttagtaaat atattaaacc aaatgaacat aatcaattag
     2581 aattagattc aaaagttaaa gaacaggtta gtccttttgt ttatgagcat tatcaaagag
     2641 gtattcaagc tattaatcag gcaactgaag caggatatgc aacgataaat gtagaaaata
     2701 ataaactgga agctactcca ttagcaaata gttctatgaa taatttagtg gggtttaatg
     2761 ctaaagatgg aattcaatat tcatctgtag attcttattg gtggggcgtt aaatggtata
     2821 tgtcaaagag tgaatcccaa caatggcaaa ataattttgc tgatgggtca ttttattggg
     2881 gaagtatagc cgctattact ggattattaa ctccattttt cccaccagca gccatagctt
     2941 cagcagtttc agtaattatg tctgttggaa attatcattg ttatagagaa ttacgcgatc
     3001 acacaagttc acgtggttca gtaatgacga ttagttggtt ggataaagaa atttacgcta
     3061 ataaaagata agtatttttt aagtaggagt tgagataatg actttcgtaa tgatacagat
     3121 tggtatagct atattcctac tttttataat tggattgatt tataaagtta tcgggaaaaa
     3181 atttaacagt aaatttagta tgtatttttt agttatcatt gtgttttttg ctttattatt
     3241 tttggtgata agtgagatta atacaaatgg atttacagat ttaaaggttt tagtgttttt
     3301 acctttaatt gtattgagtc ttattctcct tataataaat gcaataaaat caaaaaaaat
     3361 atagaacttt caacttaaat aaaagaaata aaagccactt taaagtggct tttatttctt
     3421 ttatttggtt aaccgcctgt gtttccatca gacattagca ttattttttg aatagatggg
     3481 tctttgtttt cttttggagt attgatattg cttagagtta aagtaaagac agtaataatt
     3541 cccattaatg ttaggattgt ttttttcatt ttaaagcccc cttattatga gatttttttc
     3601 ttgcttttga acttaaatag aaatatttac ttgcttttga gtgattatct tcatgataaa
     3661 attttattgc taatgtttcg ttatattcgg caatgtactc atatagattt tctttttcaa
     3721 aatagatcat tcctgctaat attatttgtt ctaatttttc tccaggtatt tcattgttta
     3781 ttgctagtaa aatcttaaat cgatgttgat attcttcaat tttgaggtta ttactaattt
     3841 ttagaccttt ttcgagaagt tctaatgctt tgtttttgtt atttagtttt gcatgttcgc
     3901 atgcttcaat taagattgct ctgtagttat ttggtgattt ttgaataact tcggataaat
     3961 agcgaattgc taattctgat aaattttgtt ttgcatacat aaatcctaga ttatgacgaa
     4021 catataaaat agcttgttct tctttttctt tttggaatat atctattgct gatataaagt
     4081 attcttcagc taattcgtat tcttttagat gtgtgcatga taatccagaa aggttattac
     4141 aatagcctat gtttaattca tatcctttgt atttagaaaa gatttctttt gctttagatg
     4201 cttgtttaat agccggaaca tattgttgat tgtggcagct aaatgtggaa agtttataat
     4261 aaaattcagc gtgttccaat tggtctggta tttcttttaa ttttatttca gctttgtcat
     4321 agtattctct tgctaagttg tagtttccga tgctagttgc gtgtatggct ttaaaaaaga
     4381 aataatagta tgagagaaga ttatctgtag gtgtgttaaa agattcaatt ttgtcgaagc
     4441 tatccttacc aatacttaaa tgatcgatta gatattggtg gcgaaaatct aatagtgaat
     4501 agtataaaag tagattttgg tcttcttcta tgttatggat ttttttatca atttctaatc
     4561 ttaatttatg cgcattacct aaatgtctag cacgaatttc gatataccag tcatttaaca
     4621 tgtttgtgat ttgttcatta ctttttgttt gaacgtccac aaaatagccc ccttttttac
     4681 gaatgtatat tatttccact tattttatca tgtcttaaca tatatctaat ttattaaatt
     4741 tgaaaaatat attttagaat attctgaaat atcaagttga aaaagatgtt aaaaagcaaa
     4801 aaagccttat aaaataagga ttttttgcta atggttgtaa taaggtattc gtcttattga
     4861 catttttagg atatcaaact tagaaaaaaa tgtatatatt taattttgaa aatataaaaa
     4921 gtcagacaag atacaacaaa aaaagccatt catgctataa ttgagttgtt cagaaacaac
     4981 tatagaagaa gggctttttt tactgttata caattaatta gagtgttcag actcaaatta
     5041 atttatctaa ctacataata acaaaataga agtctcttcg ctagttgtaa atactagaaa
     5101 cgaggagaaa agtatgccaa acactattga atttaaacag gttgagaaaa atgcaagaat
     5161 acgtgattta gaaatagaaa atgaaaaatt taagcaagat catcatgaag tttctaaaga
     5221 tgaattagaa aaagcaatgg aaactatggc aaaagcaact ggtaaagagc tttacatagg
     5281 tactaaaaaa agcccacatt cgagagtgag gtttgctcaa tcattgcaag aaaatctagg
     5341 tttcttaaat aaaaatggat atttaactaa taaagaaaaa gtatttttaa gtgatataac
     5401 accttatata gctttctcga gcaattgtat tgttcatgat attaaagcta aaaatcctgt
     5461 tcctgctaat gtttcagaga ttgcaaaatt aattggcatt tcaagacaaa atacaagctt
     5521 agcaataaat tcattagtta aaaagggtct tttattcaaa ggggattcag gtgttgaggg
     5581 aaataatgct aaagcatatg cggtctttgt aaatccacac attatttacg ctggagataa
     5641 agatagtgtg aatgaagcac ttcaagtaat gttttataag gctatgaaaa tgaaaatatt
     5701 aaaagatttg ccagataaat tattttagga ttgacaattc gtcaatccta ttttttttag
     5761 cttttcgtgt gtcatgattt catgacacat gtgtcatgat ttcgtgacac acgaaaagct
     5821 ttgtaaatac tgatatatca aggttttttt tacttttaaa actctctctc ttctcactct
     5881 atatatagag ggctaaaagt tccgcaagga acaggttgta tttttgagta aaaagcactc
     5941 aaaaatctcc acctttttca ctacgtttct tccttgcgga acttttttcg gctggttttt
     6001 agctttcgcc aagccgaaca gaccaaaaac ggggttaagt tgctcgactt ggactagcaa
     6061 ttgtaccagc cgaaccgcca attcttacgt tcaaagcatg attctttttc catacagatc
     6121 aacgctatgt ggggtttcgc ccccacacgt cgagccagca ctcacaccca aaaacagggg
     6181 agaagataat ttctcgttcc gatttagtac taaaaaggac tataatggaa ctattaaaat
     6241 agtactattt agtttattgg aggtcgtgtg aatgtctaaa caacaggtta aattctatgt
     6301 tatggaaaag gaaatggagc gtttaaaact tttgtctgaa atgcgatata tgacagttcc
     6361 taattatgca aagctaacag cattaggtgt gcaaattaga cagccaaaag agatagttgt
     6421 aggcaatgta gaattacaag aagaagataa agcgatatta aaagaaattt tggagagaag
     6481 acgaggaatt agttatatag cttatgacac aggatttaac cagcgattgg aacaactcgc
     6541 aaaacgacta ttgggagaag aaggataagc ggtaagtgtc aatggtaagc ggactggaag
     6601 ggatcagcgg tttatgctga tccctgaaag gagcattaag gaatggacac gtgcagccga
     6661 ttctccatcc tcttggcagt tcctcaaaac gaatcgtttt ggggacgcca accggcgagg
     6721 gagcccctca aggattgagg ggttggggag ttcgattggt ggggtcaagg ggaagagttc
     6781 cccttgtggg gtttggggca aagcccctgg tgtttatcac gctgtggcgt gatttggcga
     6841 agccaacaag gtctcgcaga gcccacacca aatggaattt
//
