LOCUS       NC_018652               1549 bp    DNA     circular CON 07-MAY-2024
DEFINITION  Escherichia coli O104:H4 str. 2009EL-2050 plasmid pG-09EL50,
            complete sequence.
ACCESSION   NC_018652
VERSION     NC_018652.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN01831189
            Assembly: GCF_000299255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O104:H4 str. 2009EL-2050
  ORGANISM  Escherichia coli O104:H4 str. 2009EL-2050
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ahmed,S.A., Awosika,J., Baldwin,C., Bishop-Lilly,K.A., Biswas,B.,
            Broomall,S., Chain,P.S., Chertkov,O., Chokoshvili,O., Coyne,S.,
            Davenport,K., Detter,J.C., Dorman,W., Erkkila,T.H., Folster,J.P.,
            Frey,K.G., George,M., Gleasner,C., Henry,M., Hill,K.K., Hubbard,K.,
            Insalaco,J., Johnson,S., Kitzmiller,A., Krepps,M., Lo,C.C., Luu,T.,
            McNew,L.A., Minogue,T., Munk,C.A., Osborne,B., Patel,M.,
            Reitenga,K.G., Rosenzweig,C.N., Shea,A., Shen,X., Strockbine,N.,
            Tarr,C., Teshima,H., van Gieson,E., Verratti,K., Wolcott,M., Xie,G.,
            Sozhamannan,S. and Gibbons,H.S.
  CONSRTM   Threat Characterization Consortium
  TITLE     Genomic comparison of Escherichia coli O104:H4 isolates from 2009
            and 2011 reveals plasmid, and prophage heterogeneity, including
            shiga toxin encoding phage stx2
  JOURNAL   PLoS One 7 (11), e48228 (2012)
   PUBMED   23133618
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Johnson,S.L., Teshima,H., Chertov,O., Gibbons,H.S.,
            Bishop-Lily,K.A., Strockbine,N., Minogue,T., Rosenzweig,N.,
            Sozhamannan,S. and Detter,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2012) Genome Science B6, Los Alamos National
            Laboratory, PO Box 1663 M888, Los Alamos, NM 87545, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000299255.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 02:05:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,395
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 4,972
            CDSs (with protein)               :: 4,972
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 117 of 295
            Pseudo Genes (incomplete)         :: 190 of 295
            Pseudo Genes (internal stop)      :: 55 of 295
            Pseudo Genes (multiple problems)  :: 61 of 295
            Pseudo Genes (short protein)      :: 2 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003300.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli O104:H4 str. 2009EL-2050"
                     /mol_type="genomic DNA"
                     /strain="2009EL-2050"
                     /host="Homo sapiens"
                     /db_xref="taxon:1134782"
                     /plasmid="pG-09EL50"
                     /geo_loc_name="Georgia"
                     /collection_date="2009"
     gene            1..126
                     /locus_tag="O3M_RS27635"
     CDS             1..126
                     /locus_tag="O3M_RS27635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001431667.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
                     /name="hypothetical protein"
     gene            complement(571..1047)
                     /locus_tag="O3M_RS27645"
                     /old_locus_tag="O3M_26622"
     CDS             complement(571..1047)
                     /locus_tag="O3M_RS27645"
                     /old_locus_tag="O3M_26622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          1127..1235
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.091"
                     /coverage="100.0"
                     /evalue="9.09e-49"
     Typing          complement(1431..1529)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          80..340
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.985"
                     /coverage="100.0"
                     /evalue="1.85e-99"
ORIGIN
        1 atgtggcttc cagttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga
       61 cttgcggcga gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg
      121 ggatgagtcg ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg
      181 ttttagatgc ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg
      241 tcaagcgcgc cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc
      301 ctgcaaagcc catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt
      361 cggtgttgca gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg
      421 cgcggccgga accgtggcgg ctcggttttt tttggagaac ggggagcaaa aacacgcaaa
      481 ggattttgag gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac
      541 gtagcccctg attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg
      601 ctgaatatca ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt
      661 agtgttgtat cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc
      721 aacggggatt ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt
      781 gcttctgcta actccaagtt tttcagctag aaattgctga tccgcaacga cagcgccaca
      841 cgtaggatcg atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct
      901 atccttcagc agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga
      961 atttttcttt gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt
     1021 cagtcgtttt tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac
     1081 gttggttcaa atctaagcca tcatgacaca tctatgcaac accttgttgc gtgttgtcgc
     1141 atccaggatg cgacaacgtt gtgtcatcct ggatgcgaca ggtaaataaa aaacacacta
     1201 aaaaaacaac cacttacaaa tcggcgtaaa agatctattc taggcaaggg tgttgcgtgc
     1261 aacgcaacgc cctttggggg tgtcggggcg aagccctgac ccagtcacgt agcgatagcg
     1321 gagtgtatac tggcttactc ctatgcaagc ctcgccagcc gtgatttttg ccagatttcg
     1381 tggttttcac gttcaacagc tccatcgttt ttttgccgtc cgttcaccgt taaaacgcct
     1441 cagaaatcgg gacaggatgt gtaatcgtat aaccgcgcat gcacgccgat acgattacca
     1501 cttggtcagg gcttcacccc gacaccccgc atcagtgcgt aagtgctca
//
