LOCUS       NC_017655               5954 bp    DNA     circular CON 27-MAR-2024
DEFINITION  Escherichia coli O55:H7 str. RM12579 plasmid p12579_5, complete
            sequence.
ACCESSION   NC_017655
VERSION     NC_017655.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02604278
            Assembly: GCF_000245515.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O55:H7 str. RM12579
  ORGANISM  Escherichia coli O55:H7 str. RM12579
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Kyle,J.L., Cummings,C.A., Parker,C.T., Quinones,B., Vatta,P.,
            Newton,E., Huynh,S., Swimley,M., Degoricija,L., Barker,M.,
            Fontanoz,S., Nguyen,K., Patel,R., Fang,R., Tebbs,R., Petrauskene,O.,
            Furtado,M. and Mandrell,R.E.
  TITLE     Escherichia coli serotype O55:H7 diversity supports parallel
            acquisition of bacteriophage at Shiga toxin phage insertion sites
            during evolution of the O157:H7 lineage
  JOURNAL   J Bacteriol 194 (8), 1885-1896 (2012)
   PUBMED   22328665
REFERENCE   2  (bases 1 to 5954)
  AUTHORS   Kyle,J.L., Cummings,C.A., Parker,C.T., Quinones,B., Vatta,P.,
            Newton,E., Huynh,S., Swimley,M.S., Degoricija,L., Barker,M.,
            Fontanoz,S., Nguyen,K., Patel,R., Fang,R., Tebbs,R., Petrauskene,O.,
            Furtado,M. and Mandrell,R.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2011) Western Regional Research Center / Produce
            Safety and Microbiology Research Unit, United States Department of
            Agriculture / Agricultural Research Service, 800 Buchanan Street,
            Albany, CA 94710, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000245515.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 17:42:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,362
            CDSs (total)                      :: 5,231
            Genes (coding)                    :: 4,984
            CDSs (with protein)               :: 4,984
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 102
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 95 of 247
            Pseudo Genes (incomplete)         :: 131 of 247
            Pseudo Genes (internal stop)      :: 73 of 247
            Pseudo Genes (multiple problems)  :: 45 of 247
            Pseudo Genes (short protein)      :: 4 of 247
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003114.1.
            Genome was manually curated based on annotation generated by the
            NCBI Prokaryotic Genomes Automatic Annotation Pipeline Group.
            Information about the Pipeline can be found here:
            http://www.ncbi.nlm.nih.gov/genomes/static/Pipeline.html.
            Bacteria available from Dr. Robert Mandrell
            (robert.mandrell@ars.usda.gov).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Escherichia coli O55:H7 str. RM12579"
                     /mol_type="genomic DNA"
                     /strain="RM12579"
                     /serotype="O55:H7"
                     /isolation_source="urine from child < 5 years old"
                     /host="Homo sapiens"
                     /db_xref="taxon:1048689"
                     /plasmid="p12579_5"
                     /geo_loc_name="USA: California"
                     /collection_date="1974-12"
     gene            join(5847..5954,1..129)
                     /locus_tag="ECO55CA74_RS31400"
     CDS             join(5847..5954,1..129)
                     /locus_tag="ECO55CA74_RS31400"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_072037177.1"
                     /translation="MENLNKTVAFIIKKYRHEASFSQEELSDLANIHRTYLSQIERGLK
                     CPTLHVLNDLSKALRIPLSVIIREMELYELQNK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            110..775
                     /locus_tag="ECO55CA74_RS31890"
                     /old_locus_tag="ECO55CA74_26419"
     CDS             110..775
                     /locus_tag="ECO55CA74_RS31890"
                     /old_locus_tag="ECO55CA74_26419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015168488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001106952.1"
                     /translation="MNYKINNRFSIDVGFLVNYEHIRKSIIQTNDILSNLPSTLFNSID
                     YKTTSAMIGSIYCDTLALLTNSIVNPIEKGHPDILPKSALNATEERLRNYPQGLEIKCT
                     VGNITTGANLRAGQNRIDYINGITWQAHHQEVSTLMGLVWDFVDCGKSFFYPTITAVFY
                     CNNLTPEDWGAVSGTTGRNTKVSGMLTSGKRKMASGWIAVIDNPKYINTYGKVLNFNL"
                     /name="hypothetical protein"
     gene            complement(765..2024)
                     /locus_tag="ECO55CA74_RS27565"
                     /old_locus_tag="ECO55CA74_26424"
     CDS             complement(765..2024)
                     /locus_tag="ECO55CA74_RS27565"
                     /old_locus_tag="ECO55CA74_26424"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_000622333.1"
                     /translation="MISASLFSGAGFGDMGFRAMGFFPALLCEIDPKRTAFTKINFPQS
                     EVLTCDISENVENIIDRINKILEQKKEDKLFLLYATPPCQGMSKNGIGSILKAMADNKR
                     PQIDKRNHLYLPVIDIVRSVKPKWIFFENVCRLFNFNDIDKNGKVEKITKIIENEFREI
                     GYAGKFEQVQMADYGLPQTRLRSVGIFRLNEDSFFKKDESFIPKPTIKNPKNRITLRSV
                     ISKNEELIAIDPKKRMSSLNPLHRVPKWRDDLDFWMRNTPEGCSAFDNNTCLSCSFVNK
                     KIDVRCTSCGSLLPKPVIVKNGEVKLIKGFVSAYKRMYWDKPASTITTRSAYACSDHKV
                     HPSENRVLSIYEIAQLQGIDIDEVIWKDSTGKQFSDTLLRELIGESVPPLFTKIIAKKI
                     VEIEKSRSTIINNKECFFTD"
                     /name="DNA cytosine methyltransferase"
     gene            complement(2358..2513)
                     /locus_tag="ECO55CA74_RS32565"
     CDS             complement(2358..2513)
                     /locus_tag="ECO55CA74_RS32565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_226224659.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAFDKSRCAREKF
                     REYLKW"
                     /name="replication initiation protein"
     gene            complement(3182..3373)
                     /locus_tag="ECO55CA74_RS27465"
                     /old_locus_tag="ECO55CA74_26429"
     CDS             complement(3182..3373)
                     /locus_tag="ECO55CA74_RS27465"
                     /old_locus_tag="ECO55CA74_26429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165976.1"
                     /translation="MTKQEKTALNMARFIRNQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3437..3760
                     /locus_tag="ECO55CA74_RS27470"
                     /old_locus_tag="ECO55CA74_26434"
     CDS             3437..3760
                     /locus_tag="ECO55CA74_RS27470"
                     /old_locus_tag="ECO55CA74_26434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956006.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGESVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQMVREQRVQ
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3969..>4400
                     /locus_tag="ECO55CA74_RS32570"
                     /pseudo=""
     CDS             3969..>4400
                     /locus_tag="ECO55CA74_RS32570"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4438..4956
                     /locus_tag="ECO55CA74_RS31810"
                     /old_locus_tag="ECO55CA74_26444"
     CDS             4438..4956
                     /locus_tag="ECO55CA74_RS31810"
                     /old_locus_tag="ECO55CA74_26444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_014640501.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGQIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            4963..5196
                     /locus_tag="ECO55CA74_RS31815"
                     /old_locus_tag="ECO55CA74_26449"
     CDS             4963..5196
                     /locus_tag="ECO55CA74_RS31815"
                     /old_locus_tag="ECO55CA74_26449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137438.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          3012..3097
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.851"
                     /coverage="100.0"
                     /evalue="3.99e-36"
     Typing          complement(2442..2990)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.404"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3751..5300
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.363"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttaagtcaaa tagagcgggg tttaaagtgc ccaacgctcc atgttttgaa cgatttatct
       61 aaagcgctac gcataccact ttcggtaatt attagagaaa tggaattata tgaactacaa
      121 aataaataac agatttagta tcgatgtcgg tttcttagtg aattatgagc atattcggaa
      181 gtctataatt caaacaaatg atatattaag taatttacca tcaaccttgt ttaacagtat
      241 tgattataaa acaacaagtg caatgattgg gtctatctat tgtgatactt tggcattatt
      301 aactaactct attgttaacc caatagaaaa agggcaccct gatattttac ctaaaagcgc
      361 cttaaacgct acagaagagc ggctgagaaa ctatccacaa gggcttgaga taaagtgtac
      421 tgttggtaac ataacaactg gagcgaattt acgcgcagga caaaacagaa tcgattacat
      481 taacggtatt acgtggcaag ctcatcatca agaagtatct acattaatgg gattggtttg
      541 ggatttcgtt gattgtggga agtcattttt ttatcctact attaccgcag tcttttactg
      601 taataattta acccccgaag actggggggc tgtcagcggc acaacgggaa gaaatactaa
      661 agtttctgga atgttaacta gtgggaaaag aaaaatggca agtggttgga ttgccgttat
      721 agacaatcca aagtatatca atacatatgg aaaagtattg aactttaatc tgtaaagaaa
      781 cactctttat tattgataat agttgatctt gatttttcaa tttcaactat tttttttgca
      841 atgattttgg taaacagtgg tggaacgctt tcacctatca attcacgaag aagcgtgtca
      901 gaaaactgtt ttcctgttga gtctttccag attacctcat ctatatcaat gccttgtagt
      961 tgtgctattt catatataga taaaactcta ttctcacttg ggtgaacctt atgatctgag
     1021 catgcataag cagatctggt cgttatagtc gatgcaggtt tatcccaata cattctttta
     1081 tatgcggaaa caaaaccttt tattaattta acttctccat tttttactat cactggtttt
     1141 ggtaataagg aaccacaaga tgtacatctt acatcaatct ttttattcac aaaagagcat
     1201 gataagcaag tgttattatc aaaggcagaa cacccttcag gggtgtttcg catccaaaag
     1261 tctagatcat ccctccattt cggtactcta tgcaatgggt ttaaagatga cattctcttt
     1321 ttaggatcaa ttgctatcaa ttcttcattt tttgatatca cacttcttaa cgttattcta
     1381 tttttcggat ttttaatcgt aggttttggt atgaaacttt catccttttt aaaaaaactg
     1441 tcttcgttta acctaaaaat acctacgctt cttaagcgtg tttgaggtaa accgtagtca
     1501 gccatttgaa cttgttcaaa tttaccagca taaccaattt ctctaaattc attttctatt
     1561 atttttgtta ttttttctac ttttccattt ttatcaatgt cgttaaagtt aaatagtcgg
     1621 cagacatttt caaaaaaaat ccattttggt tttacagatc ttacaatatc tataactggc
     1681 aaatatagat gatttctttt gtcgatctgt ggccttttat tatctgccat agctttaagg
     1741 atagatccga ttccattttt agacatgcct tgacatggtg gtgtggcata tagaaggaaa
     1801 agtttatctt cttttttttg ttctagtatt ttattaattc tatcgattat gttttccaca
     1861 ttttccgata tgtcacaagt taatacttcc gattgtggga aatttatttt tgtaaatgca
     1921 gttctttttg gatctatttc acataacagc gccgggaaaa atcccatcgc tctaaaaccc
     1981 atatcgccaa agccagcgcc agaaaacaaa cttgctgata tcatataaaa ctccgtactt
     2041 cagtattgaa tactatagta ttctattgat gtttcgaaaa caagtactaa cgcacatagt
     2101 actattatgt atattcaatc cgggtggctg attttgtatg ttgcgtgatg tgatggtggg
     2161 ctgttttcta gggatccaag taagatcctc ttgagatcga tggctgctgc gcgtaatctc
     2221 ttgccttgta aacgaaaaaa ccgccttgca gggcggtttg atcgaaggtt aagccagctt
     2281 gcagactgtt taaccaggta actgtcttgt gcgagacgag tcaccaaaac tgtcctttca
     2341 gtgtagccgg actccggtca ccacttcaag tactctctaa atttctctct cgcacaccga
     2401 cttttatcaa aggctgctgc cagtggcgtt aagtcgtgtc ttaccgggtt ggactcaaga
     2461 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2521 agcttggagc gaacgaccta caccgaactg agataccaac agcgtgagct atgagaaagc
     2581 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2641 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2701 tttcgccacc tctgacttga gcgtcggttt ttgtgatgct cgtcaggggg gcggagccta
     2761 tggaaaaacg ccagcggcgt ggccttttcc gggttcttgt tttttgctca catgtttctt
     2821 ttgcggttat cccctgattc tgtggataac cgcattaccg tctttgagtg agctgatacc
     2881 gctcgccgca gccgaacggc cgagcgtagc gagtcagtga gcgaggaagc ggaagagcgc
     2941 ctgatgcggc attttctcct tacgcatctg tgcggtattt cacaccgcat atggtactct
     3001 cttcttataa tctgcactga tgccacatag ttaagccagt atacactccg ctatcgctac
     3061 gtgactgggt catggctgcg ccccgacacc cgctaaaacc cgctgacgcg ccctgacggg
     3121 cttgtctgct cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgtatgt
     3181 gtcagaggtt ttcaccgtca tccccgaagc gtgcgaggca gctgcggtaa agctcatcgg
     3241 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagt tcgttgagct
     3301 tttccagcag cgttaatgtc tggtttctga taaagcgcgc catgttaagg gctgtttttt
     3361 cctgtttagt cactgatgcc tccgtgtaag ggggatttct gttcatgggg taatgatacc
     3421 gatgaaacgc gagaggatgc tcacgatacg ggttactgat gatgaacatg cacggttact
     3481 tgaacgctgt gagggtaaac aactggcggt atggatgcgg cgggtctgcc tgggggagtc
     3541 ggttgcccgt tccgggaagc tgccgacact ggcaccgccg ttactgcgtc agctggccgc
     3601 catcgggaat aacctgaatc agacggcgag gaaggtgaac agcgggcagt ggtcttccgg
     3661 tgaccgggtt caggtggtgg ccgcactgat ggccatcgag agagagttgc gcagcttacg
     3721 gcagatggtc agggaacaga gagtgcagga tgatagttaa gtttcatgcc aggggaaaag
     3781 gtggtggcag tggtccggtt gattacctgc tgggaaggga gcgaaaccgg gaaggggcaa
     3841 cggtgcttca gggtaatccg gaagaagtcc gggaactcat cgatgccacg ccatttgcga
     3901 agaaatacac gtcaggtgtt ctgtcgttcg cggagaagga gctgccgccg ggaggacgtg
     3961 aaaaggtgat ggcgagcttt gagcgtgtac tgatgcccgg tctcgaaaaa aatcagtaca
     4021 gcatcctgtg ggtagagcac caggacaagg gacggcttga gcttaatttt gtcattccga
     4081 acatggagct acagagcgga aaacgcctcc agccgtacta cgaccgggca gacaggccga
     4141 gaattgatgc ctggcagacg ctggtgaatc accattacgg gctgcatgac cccaacgccc
     4201 cggagaaccg ccggatactg actctccctg ataacctgcc ggaaacgaaa caggcgcttg
     4261 cagagagcgt cacacgtggg atagacgctc tttatcatgt cggggagata aaaggccgtc
     4321 aggatgtgat tcaggcgctc acagaggcag ggcttgaagt ggtcagggtg acgcgaagca
     4381 gtatcagcat tgcagacccg aacggcggga agaatatcag gctgaaagga gcattctatg
     4441 agcaatcttt tacagacggg cgcggagttc gagaaaaagc tgaaagagag agccgaatct
     4501 acagagacaa tgctgaacga cgagttcagc aagctcgaaa aatctgtaag caaggctgtg
     4561 acatcaaacg agacgaaaat caaagacgct atagccctgt tcacagcttc gacagaggaa
     4621 tcactgaaaa aacaccggga aggggtgaaa gaggcgatga tgcagcacag gaaggacgtg
     4681 ttaaagctgg cagggaatac gggcatgatg ttactgggga tggtcatttt cctgtttacc
     4741 gtgagtggcg ggacgctctg gtatcttggc gggcagatac aggcgaacct ggaagaaatc
     4801 aggatacagg aagaaacatt gcagaaactg aacgcgaaga catggggcgt ggagtttgtg
     4861 caggacggga gaaggaaatt tctggtcatc ccgcagggga aatcggcaac ggtgattccc
     4921 tatcagggga aggactgggt acaactgacg gagtgacaca gagtgacgga gctggaaaga
     4981 catttgctga acgccttaga gcaactgcaa caggactata tgcagcggct gaacgaatgg
     5041 gagagcgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga caacgcgatg
     5101 ctgaacgagc aggtcatgca gttgagtcag caggtgcagc acttgagcga gcggacagaa
     5161 cgcttgagcc agttatacag cgagaacagg agataaggga cgaacggctg atacaggagc
     5221 gcgaacatgc gttatccctg gagcgtgagc gccagccgga aatacaggag cgcacactgg
     5281 atggcccttc gctgggatgg taatcagaga caggctaaaa tgttgtcagc gcgtttctgg
     5341 tgcatttttt gggataatgg ttggttcggg ggcaccggga acgcgcttca gtcagacctt
     5401 gctgaaaaaa caggaacgcg ttaaaacgca tacgggcgcg attctggtgt atcttttgtt
     5461 gctgtgtttt gcctctattg ccagatcgcg ctcattcccc tgaacttctg ttttcccgct
     5521 ccggttcccg tcttcttcgc ttccgaaagc atgaaaaaaa tccgtttcag cagtctctgg
     5581 ctggaaattc ctgcgtgatt cacatggctg catagctatg catgtggtta aaatttaccg
     5641 ggtgcgatcg cggcagtttt tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg
     5701 atcgcaccga acgcgtttca gtggtgcgta caattgaaat gttatggtaa atagctttta
     5761 taacatcctg attaacttaa atcgttttca aaatgaatac tattgtctgt agagcaatgg
     5821 tattcacatg gtggattatt gtagttatgg aaaatttaaa taaaaccgta gcttttatta
     5881 taaaaaaata tcgacatgaa gcttcctttt ctcaggagga gttatctgat ttagcgaata
     5941 tacatagaac ttat
//
