LOCUS       NC_017654              12068 bp    DNA     circular CON 27-MAR-2024
DEFINITION  Escherichia coli O55:H7 str. RM12579 plasmid p12579_3, complete
            sequence.
ACCESSION   NC_017654
VERSION     NC_017654.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02604278
            Assembly: GCF_000245515.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O55:H7 str. RM12579
  ORGANISM  Escherichia coli O55:H7 str. RM12579
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12068)
  AUTHORS   Kyle,J.L., Cummings,C.A., Parker,C.T., Quinones,B., Vatta,P.,
            Newton,E., Huynh,S., Swimley,M., Degoricija,L., Barker,M.,
            Fontanoz,S., Nguyen,K., Patel,R., Fang,R., Tebbs,R., Petrauskene,O.,
            Furtado,M. and Mandrell,R.E.
  TITLE     Escherichia coli serotype O55:H7 diversity supports parallel
            acquisition of bacteriophage at Shiga toxin phage insertion sites
            during evolution of the O157:H7 lineage
  JOURNAL   J Bacteriol 194 (8), 1885-1896 (2012)
   PUBMED   22328665
REFERENCE   2  (bases 1 to 12068)
  AUTHORS   Kyle,J.L., Cummings,C.A., Parker,C.T., Quinones,B., Vatta,P.,
            Newton,E., Huynh,S., Swimley,M.S., Degoricija,L., Barker,M.,
            Fontanoz,S., Nguyen,K., Patel,R., Fang,R., Tebbs,R., Petrauskene,O.,
            Furtado,M. and Mandrell,R.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2011) Western Regional Research Center / Produce
            Safety and Microbiology Research Unit, United States Department of
            Agriculture / Agricultural Research Service, 800 Buchanan Street,
            Albany, CA 94710, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000245515.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 17:42:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,362
            CDSs (total)                      :: 5,231
            Genes (coding)                    :: 4,984
            CDSs (with protein)               :: 4,984
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 102
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 95 of 247
            Pseudo Genes (incomplete)         :: 131 of 247
            Pseudo Genes (internal stop)      :: 73 of 247
            Pseudo Genes (multiple problems)  :: 45 of 247
            Pseudo Genes (short protein)      :: 4 of 247
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003112.1.
            Genome was manually curated based on annotation generated by the
            NCBI Prokaryotic Genomes Automatic Annotation Pipeline Group.
            Information about the Pipeline can be found here:
            http://www.ncbi.nlm.nih.gov/genomes/static/Pipeline.html.
            Bacteria available from Dr. Robert Mandrell
            (robert.mandrell@ars.usda.gov).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12068
                     /organism="Escherichia coli O55:H7 str. RM12579"
                     /mol_type="genomic DNA"
                     /strain="RM12579"
                     /serotype="O55:H7"
                     /isolation_source="urine from child < 5 years old"
                     /host="Homo sapiens"
                     /db_xref="taxon:1048689"
                     /plasmid="p12579_3"
                     /geo_loc_name="USA: California"
                     /collection_date="1974-12"
     gene            89..691
                     /locus_tag="ECO55CA74_RS27370"
     CDS             89..691
                     /locus_tag="ECO55CA74_RS27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071469.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="WP_000333347.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTELG
                     RNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQ
                     KRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGLTLSGTKTHVKGFWS"
                     /name="IS4 family transposase"
     gene            complement(786..1646)
                     /locus_tag="ECO55CA74_RS27375"
                     /old_locus_tag="ECO55CA74_26309"
     CDS             complement(786..1646)
                     /locus_tag="ECO55CA74_RS27375"
                     /old_locus_tag="ECO55CA74_26309"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(1829..2386)
                     /locus_tag="ECO55CA74_RS27380"
                     /old_locus_tag="ECO55CA74_26314"
     CDS             complement(1829..2386)
                     /locus_tag="ECO55CA74_RS27380"
                     /old_locus_tag="ECO55CA74_26314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            2550..5555
                     /locus_tag="ECO55CA74_RS27385"
                     /old_locus_tag="ECO55CA74_26319"
     CDS             2550..5555
                     /locus_tag="ECO55CA74_RS27385"
                     /old_locus_tag="ECO55CA74_26319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143760.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            <5590..6252
                     /locus_tag="ECO55CA74_RS27735"
                     /pseudo=""
     CDS             <5590..6252
                     /locus_tag="ECO55CA74_RS27735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /name="IS4-like element ISVsa5 family transposase"
     gene            6336..6686
                     /locus_tag="ECO55CA74_RS27395"
                     /old_locus_tag="ECO55CA74_26329"
     CDS             6336..6686
                     /locus_tag="ECO55CA74_RS27395"
                     /old_locus_tag="ECO55CA74_26329"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000348887.1"
                     /translation="MDGLSKGSPLSTTYLALWFRVSDEGLIEIRDKAALAFESGFASER
                     GVTTWAGRMKKLKELGFISCREGSTGEFHYVLIVHPLVAVKKLLDEGIIPKGKTYNILS
                     ERVIEVGASWEG"
                     /name="hypothetical protein"
     gene            6687..7181
                     /locus_tag="ECO55CA74_RS27400"
                     /old_locus_tag="ECO55CA74_26334"
     CDS             6687..7181
                     /locus_tag="ECO55CA74_RS27400"
                     /old_locus_tag="ECO55CA74_26334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000929317.1"
                     /translation="MAGTVWRDIRTGETVFPMGHLHPQRCCVDVNGTSVDIEISFGFHV
                     FTDEKQTGMLMKFKEEQRFFCRERYEGSKTIVHRILTAIENGEYITAFISKGQGQRYYH
                     LSHHDDFILMEIRKPQDRNNSLRIHVVTAYTLDEWGTVNKGRNLRFRYVLEQRLQGKKI
                     V"
                     /name="hypothetical protein"
     gene            complement(8728..8919)
                     /locus_tag="ECO55CA74_RS27410"
                     /old_locus_tag="ECO55CA74_26349"
     CDS             complement(8728..8919)
                     /locus_tag="ECO55CA74_RS27410"
                     /old_locus_tag="ECO55CA74_26349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8984..9307
                     /locus_tag="ECO55CA74_RS27415"
                     /old_locus_tag="ECO55CA74_26354"
     CDS             8984..9307
                     /locus_tag="ECO55CA74_RS27415"
                     /old_locus_tag="ECO55CA74_26354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_000955995.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHGAR
                     DDS"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            9516..>9947
                     /locus_tag="ECO55CA74_RS32560"
                     /pseudo=""
     CDS             9516..>9947
                     /locus_tag="ECO55CA74_RS32560"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            9985..10503
                     /locus_tag="ECO55CA74_RS31795"
                     /old_locus_tag="ECO55CA74_26364"
     CDS             9985..10503
                     /locus_tag="ECO55CA74_RS31795"
                     /old_locus_tag="ECO55CA74_26364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067503.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     EKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            10510..10743
                     /locus_tag="ECO55CA74_RS31800"
                     /old_locus_tag="ECO55CA74_26369"
     CDS             10510..10743
                     /locus_tag="ECO55CA74_RS31800"
                     /old_locus_tag="ECO55CA74_26369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137441.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            11414..11578
                     /locus_tag="ECO55CA74_RS31940"
                     /old_locus_tag="ECO55CA74_26379"
     CDS             11414..11578
                     /locus_tag="ECO55CA74_RS31940"
                     /old_locus_tag="ECO55CA74_26379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000968835.1"
                     /translation="MMDKDTTTLKRTLAHNRAFSDNINRSGIAWCYNTEIVLAACEAIE
                     AELQRRGCL"
                     /name="hypothetical protein"
     ARG             complement(790..1646)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          8553..8636
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.07e-36"
     Typing          7890..8111
                     /name="rep_cluster_2392"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.825"
                     /coverage="87.0"
                     /evalue="1.42e-85"
     Typing          9298..10847
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.794"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttttggtaaa aatcattaag ttaaggtgga tacacatctt gtcatatgat caaatggttt
       61 cgcgaaaaat caataatcag acaacaagat gtgcgaactc gatattttac acgactctct
      121 ttaccaattc tgccccgaat tacacttaaa acgactcaac agcttaacgt tggcttgcca
      181 cgcattactt gactgtaaaa ctctcactct taccgaactt ggccgtaacc tgccaaccaa
      241 agcgagaaca aaacataaca tcaaacgaat cgaccgattg ttaggtaatc gtcacctcca
      301 caaagagcga ctcgctgtat accgttggca tgctagcttt atctgttcgg gcaatacgat
      361 gcccattgta cttgttgact ggtctgatat tcgtgagcaa aaacgactta tggtattgcg
      421 agcttcagtc gcactacacg gtcgttctgt tactctttat gagaaagcgt tcccgctttc
      481 agagcaatgt tcaaagaaag ctcatgacca atttctagcc gaccttgcga gcattctacc
      541 gagtaacacc acaccgctca ttgtcagtga tgctggcttt aaagtgccat ggtataaatc
      601 cgttgagaag ctgggttggt actggttaag tcgagtaagg ggtctgacgc tcagtggaac
      661 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
      721 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
      781 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
      841 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
      901 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
      961 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1021 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1081 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1141 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1201 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc agcagtgtta
     1261 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1321 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1381 agttgctctt gcccggcgtc aacacgggat aataccgcac cacatagcag aactttaaaa
     1441 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     1501 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     1561 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     1621 gcgacacgaa aatgttgaat actcatactc ttcctttttc aatattattg aagcatttac
     1681 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     1741 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     1801 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattttat
     1861 aaaccgtgga gcgggcaata ctgagctgat gagcaatttc cgttgcacca gtgcccttct
     1921 gatgaagcgt cagcacgacg ttcctgtcca cggtacgcct gcggccaaat ttgattcctt
     1981 tcagctttgc ttcctgtcgg ccctcattcg tgcgttctag gatcctccgg cgttcagcct
     2041 gtgccacagc cgacaggatg gtgaccacca tttgccccat atcaccgtcg gtactgatcc
     2101 cgtcatcaat gaaccggact gccacgccct gagcgtcaaa ttcctttatc agttggatca
     2161 tatcggcagt gtcgcggcca agacggtcga gcttcttaac cagaatgaca tcaccttcct
     2221 ccaccttcat cctcagcaaa tccagccctt cccggtctgt tgaactgccg gatgccttat
     2281 cggtaaatat acggtttgct ttcacacctg cgtctttgag tgctctgacc tgaagatcaa
     2341 gagactgctg actggttgag acccgagcgt aaccaaaaag tcgcataaaa atgtacctta
     2401 aatcgaatat cggacaactc atgtctatta ttacaaattt acgatttaat agacatatta
     2461 atgtaacagt tttacgatgt ccgataattt ataacatttc gtacggttgg aaaaatgtta
     2521 ctaaatgccc gtcaggcagg gaggccgata tgcccgttga ctttctgacc actgagcaga
     2581 ctgaaagcta tggcagattc accggtgaac cggatgagct tcagctggca cgatattttc
     2641 accttgatga agcagacaag gaatttatcg gaaaaagcag aggtgatcac aaccgtctgg
     2701 gcattgccct gcaaattgga tgtgtccgtt ttctgggcac cttcctcacc gatatgaatc
     2761 atattccttc cggcgtccgg cattttaccg ccagacagct cgggattcgt gatatcaccg
     2821 ttcttgcaga atacggtcag agggaaaata cccgccgtga gcatgcagcg ctgatacgtc
     2881 agcactatca gtatcgtgaa tttgcctggc cctggacatt tcgccttacc cgtcttttat
     2941 atacccggag ctggataagc aacgaacgtc ctggcctgct tttcgatctg gcgacagggt
     3001 ggcttatgca acatcgtatt attctccccg gagccactac gctgacccgg ttgatttcag
     3061 aggtaaggga aaaggcgacg ttgcgcctgt ggaacaaact ggcactgata ccgtcagccg
     3121 aacagcgttc acagctggag atgctgctgg ggccaactga ttgcagccgc ctgtctttac
     3181 tggaatcact gaaaaagggc cctgtgacca tcagtggtcc ggcgtttaat gaagcaattg
     3241 aacgctggaa aactctgaac gattttggcc tgcatgctga aaacctgagt acactcccgg
     3301 ctgtgcgcct gaaaaatctc gcacgttatg ctggtatgac ttcggtgttc aatattgcca
     3361 ggatgtcacc gcagaaaagg atggcggttc tggttgcctt tgtccttgca tgggaaacgc
     3421 tggcgctgga tgatgcattg gacgttctgg acgccatgct ggccgttatc atccgtgacg
     3481 ccagaaagat tgggcagaaa aaacggctcc gctcgctgaa ggatctggat aaatctgcat
     3541 tggcgctcgc cagcgcatgt tcgtacctgc tgaaagaaga aacaccggac gaatcgattc
     3601 gtgctgaggt gttcagctac atcccaaggc aaaagctggc tgaaatcatc acgcttgtcc
     3661 gtgaaattgc ccggccctca gacgataatt ttcatgaaga aatggtggag cagtacgggc
     3721 gcgttcgtcg tttcctgccc catctgctga ataccgttaa attttcatcc gcacctgccg
     3781 gggttaccac tctgaatgcc tgtgactacc tcagccggga gttcagctca cggcggcagt
     3841 tttttgacga cgcaccaacg gaaattatca gtcggtcatg gaaacggctg gtgattaaca
     3901 aggaaaaaca tatcacccgc aggggataca cgctctgctt tctcagtaaa ctgcaggata
     3961 gtctgaggcg gagggatgtc tacgttaccg gcagtaaccg gtggggagat cctcgtgcaa
     4021 gattactaca gggtgctgac tggcaggcaa accggattaa ggtttatcgt tctttggggc
     4081 acccgacaga cccgcaggaa gcaataaaat ctctgggtca tcagcttgat agtcgttaca
     4141 gacaggttgc tgcacgtctt tgcgaaaatg aggctgtcga actcgatgtt tctggcccga
     4201 agccccggtt gacaatttct cccctcgcca gtcttgatga gccggacagt ctgaaacgac
     4261 tgagcaaaat gatcagtgat ctactccctc cggtggattt aacggagttg ctgctcgaaa
     4321 ttaacgccca taccggattt gctgatgagt ttttccatgc tagtgaagcc agtgccagag
     4381 ttgatgatct gcccgtcagc atcagcgccg tgctgatggc tgaagcctgc aatatcggtc
     4441 tggaaccact gatcagatca aatgttcctg cactgacccg acaccggctg aactggacaa
     4501 aagcgaacta tctgcgggct gaaactatca ccagcgctaa tgccagactg gttgattttc
     4561 aggcaacgct gccactggca cagatatggg gtggaggaga agtggcatct gcagatggaa
     4621 tgcgctttgt tacgccagtc agaacaatca atgccggacc gaaccgcaaa tactttggta
     4681 ataacagagg gatcacctgg tacaactttg tgtccgatca gtattccggc tttcatggca
     4741 tcgttatacc ggggacgctg agggactcta tctttgtgct ggaaggtctt ctggaacagg
     4801 agaccgggct gaatccaacc gaaattatga ccgatacagc aggtgccagc gaacttgtct
     4861 ttggcctttt ctggctgctg ggataccagt tttctccacg cctggctgat gccggtgctt
     4921 cggttttctg gcgaatggac catgatgccg actatggcgt gctgaatgat attgccagag
     4981 ggcaatcaga tccccgaaaa atagtccttc agtgggacga aatgatccgg accgctggct
     5041 ccctgaagct gggcaaagta caggtttcag tgctggtccg ttcattgctg aaaagtgaac
     5101 gtccttccgg actgactcag gcaatcattg aagtggggcg catcaacaaa acgctgtatc
     5161 tgcttaatta tattgatgat gaagattacc gccggcgcat tctgacccag cttaatcggg
     5221 gagaaagtcg ccatgccgtt gccagagcca tctgtcacgg tcaaaaaggt gagataagaa
     5281 aacgatatac cgacggtcag gaagatcaac tgggcacact ggggctggtc actaacgccg
     5341 tcgtgttatg gaacactatt tatatgcagg cagccctgga tcatctccgg gcgcagggtg
     5401 aaacactgaa tgatgaagat atcgcacgcc tctccccgct ttgccacgga catatcaata
     5461 tgctcggcca ttattccttc acgctggcag aactggtgac caaaggacat ctgagaccat
     5521 taaaagaggc gtcagaggca gaaaacgttg cttaacgtga gttttcgttc cactgagcgt
     5581 cagaccccag taagaggaaa agtacaatat gcagacctag gagcggaaaa ctggaaacct
     5641 atcagcaact tacatgatat gtcatctagt cactcaaaga ctttaggcta taagaggctg
     5701 actaaaagca atccaatctc atgccaaatt ctattgtata aatctcgctc taaaggccga
     5761 aaaaatcagc gctcgacacg gactcattgt caccacccgt cacctaaaat ctactcagcg
     5821 tcggcaaagg agccatgggt tctagcaact aacttacctg ttgaaattcg aacacccaaa
     5881 caacttgtta atatctattc gaagcgaatg cagattgaag aaaccttccg agacttgaaa
     5941 agtcctgcct acggactagg cctacgccat agccgaacga gcagctcaga gcgttttgat
     6001 atcatgctgc taatcgccct gatgcttcaa ctaacatgtt ggcttgcggg cgttcatgct
     6061 cagaaacaag gttgggacaa gcacttccag gctaacacag tcagaaatcg aaacgtactc
     6121 tcaacagttc gcttaggcat ggaagttttg cggcattctg gctacacaat aacaagggaa
     6181 gacttactcg tggctgcaac cctactagct caaaatttat tcacacatgg ttacgctttg
     6241 gggaaattat gaggggatct ctcagtactt ggcatcgact tgaaaatgat gggtactcaa
     6301 cagttccacg ttatttgcct ttgataggcg atctcatgga tggtctgtca aaaggatcac
     6361 cattatcaac aacctacttg gctttatggt tcagggtttc tgatgagggg ttgattgaaa
     6421 taagagataa agcagcactg gcttttgaat cagggttcgc tagtgaaaga ggcgtaacta
     6481 catgggcagg gagaatgaaa aagctaaaag aactggggtt catatcctgt cgtgaaggca
     6541 gcaccggtga atttcattac gtgttaattg ttcacccatt agttgcagtc aaaaagctgc
     6601 ttgatgaagg gattatccct aaaggaaaga cttataacat tttgtcggag cgtgtaattg
     6661 aggtgggagc ctcttgggag ggttaaatgg cgggcacagt atggcgtgac ataagaacgg
     6721 gagagactgt ttttcctatg gggcacctgc accctcagag gtgctgtgtt gatgtaaacg
     6781 gtacttctgt agatatagaa attagttttg gttttcatgt ttttactgat gaaaaacaaa
     6841 ccggaatgct gatgaaattt aaggaggaac agaggttttt ttgtagggaa cgctatgagg
     6901 gcagcaaaac tatcgttcac agaatactta cagcgataga aaacggtgaa tacattacag
     6961 catttatttc aaaggggcag gggcagcggt attaccatct tagccatcat gatgacttca
     7021 ttttgatgga aataagaaag cctcaagata gaaataattc tttgcgtatt catgttgtta
     7081 cagcttacac gcttgatgaa tgggggactg taaataaagg acgtaacctg cggtttagat
     7141 atgtgctgga acagcgactg cagggaaaga agattgtgta aaaaacaaaa agcgcctaag
     7201 gcgcctttgt tttttgcaga atttcggtct ggcctttcgt tatcccgctc acaggctggg
     7261 agggtgtgac ccccggcatc agcacccctc tctcgaggtt ccggtttgtg ctgacaacat
     7321 tagtatccta ttccacatgt tcgttgtcaa actagcgtac tttttggtgt tcattggtgt
     7381 ttataggttg tcatttaccc tctttgtggt tataagtgtt tataggtttt cattgttttt
     7441 tataggtgtt catatgaagt tgcttgatag taatgaagtg tgtgagctct taggcatcag
     7501 taagtccact ttgtatcgtt ggtgcggggt gtctgaggat ttttctggct tgggtctaag
     7561 tagcggtctt cctcaggcta acgctactca gaactcttta ggcagggaaa ataggctcgt
     7621 taggagggaa ttacagcaaa acgctctcgg tgtcgaagag acgccaagcg acttccctcg
     7681 tcctttcaag atcggtcgtt cgtttaaatg gaaagacgcc gaaattatgg cttggcttga
     7741 aacgcgacgc gtttgattga tagatcttct tgagatcatt tggatctcgt cgtagtcttt
     7801 tgtcctgtaa acgaaaaaac caccatggca ggtggttttt cgaaggttat ttaatcctgg
     7861 cagattgaat aaccgtggta acagccttgt gcgagacgtg tcaccaaatc tgtcctttca
     7921 gtgtagcctc actaaggcca ccacttcaag aactttgaat acatctctcg cacatcctgt
     7981 ttaccaatgg ctaccgccac tggcgatttt tcgtgtctgt tccgggttgg actcaagatg
     8041 atagttaccg ggacaacgtg gtagtcgggc tgaacggggg gttcttgcat acagcccagc
     8101 ttggagcgaa ctgtctaaac ggaacgggac gtggtgattt aggtaaagct tccaccacaa
     8161 cacggatgcc gcaggacggg aacaggagag cgcaagaggg agccgccagg gggaaacgcc
     8221 tggtatcttt aagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8281 gctcgtcagg ggggcggagc ctatggaaaa acgccagcgg cgtggccttc tctgggttct
     8341 tgttttttgc tcacatgttt cttttggtgt tatcccctga ttctgtggat aaccgcatta
     8401 ccgtctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgt agcgagtcag
     8461 tgagcgagga agcggaagag cgtcggaagt tgcattttct ccttacacat ctgtgcggca
     8521 tttcacaccc ggcatggcgc acttttcata caatccgcac tgatgccgca tggttaagcc
     8581 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgcaagg
     8641 tcaacggcct gacgcgccct gacgggcttg tctgctcccg gcatccgctc acagacaagc
     8701 tgtgaccgtc tccgggagct gtatgtgtca gaggttttca ccgtcatccc cgaagcgtgc
     8761 gaggcagctg cggtaaagct catcggcgtg gtcgtgaagc gattcacaga tgtctgcctg
     8821 ttcatccgcg tccagttcgt tgagcttttc cagcagcgtt aatgtctggc ttctgataaa
     8881 gcgggccatg ttaagggcgg ttttttcctg tttagtcact gatgcctccg tgtaaggggg
     8941 gatttctgtt catggggtaa tgataccgat gaaacgcgag aggatgctca cgatacgggt
     9001 tactgatgat gaacatgcac ggttactgga acgctgtgag ggtaaacagc tggcggtatg
     9061 gatgcggcgg gtctgtctgg gtgagccggt tgcccgttcc gggaagctgc cgacactggc
     9121 accgccgtta ctgcgccagc tggctgccat cgggaataat ctgaatcaga cggcgaggaa
     9181 ggtgaacagc gggcagtggt cttccggtga ccgggttcag gtggtggccg cactgatggc
     9241 catcgagaga gagttgcgca gcttacggca ggtcgtcagg gaacatggag cgcgggatga
     9301 tagttaagtt tcatgccagg ggaaaaggcg gtggcagtgg tccggttgat tacctgctgg
     9361 gaagggagcg taaccgggaa ggggcaacgg tgcttcaggg taatccggaa gaagtccggg
     9421 aactcatcga tgccacgcca tttgcgaaga aatacacgtc aggtgttctg tcgttcgcgg
     9481 agaaggagct gccgccggga ggacgtgaaa aggtgatggc gagctttgag cgtgtactga
     9541 tgcccggtct cgaaaaaaat cagtacagca tcctgtgggt ggagcaccag gacaagggac
     9601 ggcttgagct gaattttgtc attccgaaca tggagctaca gagcggaaaa cgcctccagc
     9661 cgtactacga ccgggcagac aggccgagaa ttgatgcctg gcagacgctg gttaatcacc
     9721 attacgggct gcatgacccc aacgccccgg agaaccgccg gatactgact ctccctgata
     9781 acctgccgga aacgaaacag gcgcttgcag agagcgtcac acgcgggata gacgctcttt
     9841 atcatgtcgg ggagataaaa ggccgtcagg atgtgattca ggcgctcaca gaggcagggc
     9901 ttgaagtggt cagggtgacg cgaagcagta tcagcattgc agacccgaac ggcgggaaga
     9961 atatcagact gaaaggagca ttctatgagc aatcttttac agacgggcgc ggagttcgag
    10021 aaaaagctga aagagagagc cgaatctaca gagacaatgc tgaacgacga gttcagcaag
    10081 ctcgaaaaat ctgtaagcaa ggctgtgaca tcaaacgaga cgaaaatcaa agacgctata
    10141 gccctgttca cagcttcgac agaggaatca ctgaaaaaac accgggaagg ggtgaaagag
    10201 gcgatgatgc agcacaggaa ggacgtgtta aagctggcag ggaatacggg catgatgtta
    10261 ctggggatgg tccttttcct gtttaccgtg agtggcggga cgctctggta tcttggcggg
    10321 cggatacagg cgaacctgga agaaatcagg atacaggaag aaacattgca gaaactgaac
    10381 gcgaagacat ggggcgtgga gtttgtgcag gacgggagaa ggaaatttct ggtccttccg
    10441 tacgggaaat cggcaacggt gattccctat caggagaagg actgggtaca actgacggag
    10501 tgacacagag tgacggagct ggaaagacat ttgctgaacg ccttagagca actgcaacag
    10561 gactatatgc agcggctgag cgaatgggag agcgccttcg tggaattgca gaaaatgttt
    10621 tcgcttacgc aacgggacaa cgcgatgctg aacgagcagg tcatgcagtt gagtcagcag
    10681 gtgcagcact tgagcgagcg gacagaacgc ttgagccagt tatacagcga gaacaggaga
    10741 taagggacga acggctgata caggagcgcg aacatgagtt atccctggag cgtgagcgcc
    10801 agctggaaat acaggaacgg acactggatg gcccttcgct gggatggtaa tcagagacag
    10861 gctaaaatgt tgtcagcgcg tttccggtgc attttttggg ataatggttg gttcgggggc
    10921 accgggaacg cgcttcagtc agaccttgct gaaaacacag gaacgcgtta aaacgcatac
    10981 gggcgcgatt ctggtgtgtc ttttgttgct gtgttttgcc tctattgcca gatcgcgctc
    11041 attcccctga acttctgttt tcccgctccg gtccccgtct tcttcgcttc cgaaagcatg
    11101 aaaaaaatcc gtttcagctg tctctggctg gcaattcctg cgtgattcac atggctgcat
    11161 agctatgcat gtggttaaaa tttaccgggt gcgatcgcgg cagtttttcg ggtggtttgt
    11221 tgccattttt acctgtctgc tgccgtgatc gcaccgaacg cgtttctgcg gtgcgggcaa
    11281 cggatgttat ggtaaatatt ggtgctctgg gatgaagcga aaataggctt tttttcgcta
    11341 ttttagcctg ttcaggaacg gcaagagtaa atatctggga gcatgaagct cgttaacctg
    11401 aatggagatc ctgatgatgg acaaagatac caccacccta aaacgcactt tggctcataa
    11461 tcgagccttt agcgataata ttaaccgcag tggcatagcc tggtgctata acactgagat
    11521 tgtactcgct gcttgtgagg cgattgaagc cgaactacaa cggcgaggat gtttgtaaca
    11581 ataagaaaac ccgcatcagc gggtttttta gtctctgaga cgcgctaccg ctaaattatt
    11641 tttctctcta tccgccctgg cttgccccct acaaccccat ggagcacaag gagcacaggg
    11701 agaaccatcg agcacaacga gcacaatgag caccgccttc ctcttataaa tcattattaa
    11761 tcatataatt aaacaacttc attgtaatat atattacttt ttatgttttt aaatgtaatg
    11821 tttattgcga tttagcttgt ttttaactct attttttcag atgagaacaa gctgccagca
    11881 atgaataact atttgatttt attggttttt tcaataaatt agtgtccata gacaaacggg
    11941 agacgactat gtccgcaaga gcaagaaaag caggtaagag aaatcagtca gaacgacgtc
    12001 aggatttgat tgaaaaattg tgggaagacg acgaaatcga tacttggcac tgatgaatcc
    12061 cctaatga
//
