LOCUS       NC_017650               1549 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Escherichia coli O7:K1 str. CE10 plasmid pCE10D, complete sequence.
ACCESSION   NC_017650
VERSION     NC_017650.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02603406
            Assembly: GCF_000227625.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O7:K1 str. CE10
  ORGANISM  Escherichia coli O7:K1 str. CE10
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Lu,S., Zhang,X., Zhu,Y., Kim,K.S., Yang,J. and Jin,Q.
  TITLE     Complete genome sequence of the neonatal-meningitis-associated
            Escherichia coli strain CE10
  JOURNAL   J Bacteriol 193 (24), 7005 (2011)
   PUBMED   22123760
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Lu,S., Yang,J. and Jin,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2011) Microbial Genome Center of Chinese Ministry
            of Public Health, Institute of Pathogen Biology, CAMS&PUMC, 6
            Rongjing Eastern Street, BDA, Beijing 100176, P.R.China
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000227625.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 01:26:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,187
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 259
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 259
            Pseudo Genes (frameshifted)       :: 92 of 259
            Pseudo Genes (incomplete)         :: 152 of 259
            Pseudo Genes (internal stop)      :: 66 of 259
            Pseudo Genes (multiple problems)  :: 42 of 259
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003038.1.
            Bacteria/DNA are available from Dr. Kwang Sik Kim
            (kwangkim@jhmi.edu).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli O7:K1 str. CE10"
                     /mol_type="genomic DNA"
                     /strain="CE10"
                     /serotype="O7:K1:NM"
                     /isolation_source="cerebrospinal fluid of a neonate with
                     meningitis"
                     /host="Homo sapiens"
                     /db_xref="taxon:1072459"
                     /plasmid="pCE10D"
                     /geo_loc_name="USA"
                     /note="isolated in the 1970's"
     gene            914..1390
                     /locus_tag="CE10_RS27215"
                     /old_locus_tag="CE10_D1"
     CDS             914..1390
                     /locus_tag="CE10_RS27215"
                     /old_locus_tag="CE10_D1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          433..531
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          complement(727..835)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.182"
                     /coverage="100.0"
                     /evalue="4.23e-47"
     Typing          complement(73..333)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.985"
                     /coverage="100.0"
                     /evalue="1.85e-99"
ORIGIN
        1 ctaccggaaa ccgtctgggg agaaccccag acccccatcg ctgcaacacc gacggtctac
       61 gaccacctac caaccgcatg gctgcctgtt ccgtgcaaat gggctttgca gggggctggc
      121 gcggttcctg gcgacctgaa agcccctggt taggggtatc ggcgcgcttg acctcctgag
      181 agcctctgta agcgtttttc gcgccttcca tgccctggcg ggcatctaaa acccgtttcg
      241 tgcgtctgag agcttttgag cgcgtttgag gggctatctg gcgactcatc cccaaaagaa
      301 caacaccggg atcaccgaca acatgcgaga ggtgatcccg ctcgccgcaa gtcgatgacc
      361 gagcgtagcg agcgaatcga cgaggaagcg gaagagaacc ggaagccaca ttgagcactt
      421 acgcactgat gcggggtgtc ggggtgaagc cctgaccaag tggtaatcgt atcggcgtgc
      481 atgcgcggtt atacgattac acatcctgtc ccgatttctg aggcgtttta acggtgaacg
      541 gacggcaaaa acacgatgga gctgttgaac gtgaaaacca cgaaatctgg caaaaatcac
      601 ggctggcgag gcttgcatag gagtaagcca gtatacactc cgctatcgct acgtgactgg
      661 gtcagggctt cgccccgaca cccccaaagg gcgttgcgtt gcacgcaaca cccttgccta
      721 gaatagatct tttacgccga tttgtaagtg gttgtttttt tagtgtgttt tttatttacc
      781 tgtcgcatcc aggatgacac aacgttgtcg catccaggat gcgacaacac gcaacaaggt
      841 gttgcacaga tgtgtcatga tggcttagat ttgaaccaac gttagtgcat gggattttca
      901 gagggaaaaa atcatgttta ttgattcaga aaaacgactg aaacaacttt cagatgaggc
      961 aaagaaaaac accgaggatc tcgaagaagc aaagaaaaat tcaaggttta cacaggtatc
     1021 cccaaaaggt tgggaacgtg ttcgagagct gctgaaggat agccaaggca tatcagcact
     1081 gaagctgtac tcatttttag cggagcatat cgatcctacg tgtggcgctg tcgttgcgga
     1141 tcagcaattc ctagctgaaa aacttggagt tagcagaagc acaattattc ggtggctcaa
     1201 ttacttagaa tcaaaaaatg cattagttag aatccccgtt gctggtaagg tttgtgcgta
     1261 tgccctcgat ccacatgaag tctggaaggg atacaacact acgaaaaacc atgcagcgtt
     1321 tgtcactaaa acactggtca acaaagacgg tgatattcag cgccgaatca tggccatgtt
     1381 ttcaaattga gctagcggca ggcggacaat caggggctac gtgttaacgt tctgaccatg
     1441 attgtctatc ctgcattgct cttttgccgc ctcaaaatcc tttgcgtgtt tttgctcccc
     1501 gttctccaga aaaaaccgag ccgccacggt tccggcagcg ccttgagcg
//
