LOCUS       NC_017649               4197 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Escherichia coli O7:K1 str. CE10 plasmid pCE10C, complete sequence.
ACCESSION   NC_017649
VERSION     NC_017649.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02603406
            Assembly: GCF_000227625.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O7:K1 str. CE10
  ORGANISM  Escherichia coli O7:K1 str. CE10
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Lu,S., Zhang,X., Zhu,Y., Kim,K.S., Yang,J. and Jin,Q.
  TITLE     Complete genome sequence of the neonatal-meningitis-associated
            Escherichia coli strain CE10
  JOURNAL   J Bacteriol 193 (24), 7005 (2011)
   PUBMED   22123760
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Lu,S., Yang,J. and Jin,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2011) Microbial Genome Center of Chinese Ministry
            of Public Health, Institute of Pathogen Biology, CAMS&PUMC, 6
            Rongjing Eastern Street, BDA, Beijing 100176, P.R.China
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000227625.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 01:26:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,187
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 259
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 259
            Pseudo Genes (frameshifted)       :: 92 of 259
            Pseudo Genes (incomplete)         :: 152 of 259
            Pseudo Genes (internal stop)      :: 66 of 259
            Pseudo Genes (multiple problems)  :: 42 of 259
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003037.1.
            Bacteria/DNA are available from Dr. Kwang Sik Kim
            (kwangkim@jhmi.edu).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Escherichia coli O7:K1 str. CE10"
                     /mol_type="genomic DNA"
                     /strain="CE10"
                     /serotype="O7:K1:NM"
                     /isolation_source="cerebrospinal fluid of a neonate with
                     meningitis"
                     /host="Homo sapiens"
                     /db_xref="taxon:1072459"
                     /plasmid="pCE10C"
                     /geo_loc_name="USA"
                     /note="isolated in the 1970's"
     gene            complement(192..353)
                     /locus_tag="CE10_RS31365"
     CDS             complement(192..353)
                     /locus_tag="CE10_RS31365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596883.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRARFI
                     QMLTEFLK"
                     /name="replication initiation protein"
     gene            complement(1019..1213)
                     /locus_tag="CE10_RS27195"
     CDS             complement(1019..1213)
                     /locus_tag="CE10_RS27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041635.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQATMCERLHELA
                     EKLQNSIQIRFEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2031..3170)
                     /gene="dcm"
                     /locus_tag="CE10_RS27200"
                     /old_locus_tag="CE10_C1"
     CDS             complement(2031..3170)
                     /gene="dcm"
                     /locus_tag="CE10_RS27200"
                     /old_locus_tag="CE10_C1"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131511.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="WP_000131511.1"
                     /translation="MTDNIAATIKEKRERLHMTQKEFADALGLSKYGDRTIRRWERGET
                     KPTGAELKAVIDFPDTPPYPNNENGRYRMIDLFAGIGGTRLGFHQTNAVNVVFSSEWDK
                     FAQKTYHANYGDFPDGDITKIDEKDIPDHEILVGGFPCVAFSQAGLKKGFNDTRGTLFF
                     DIARIIKEKKPHAFLLENVKNLLGHDKGRTFSIIKNTLEELNYTVYYNIFAAKDFGVPQ
                     NRERIYIVGFNKEKVRNHEHFTFPTPLKTKTRVGDILEKSVDNKYTLSDALWNGHQRRK
                     LVNAAAGKGFGYGLFNENSPYTNTISARYYKDGSEILIEQKGSNPRKITPREASRLQGF
                     PSDFIIPVSDTQAYKQFGNSVAVPVINAIAEKIISTLDS"
                     /name="dcm"
     gene            3241..4197
                     /locus_tag="CE10_RS27205"
                     /old_locus_tag="CE10_C2"
     CDS             3241..4197
                     /locus_tag="CE10_RS27205"
                     /old_locus_tag="CE10_C2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189381.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="WP_014640333.1"
                     /translation="MKKLSSRYKRLFFMQRLSPGEFKTLISKERKSHFITPFALVYKTF
                     CDLGYDQKNSDYFLNNPSEYIIAMRKNCWKEFEPFEKEFTTRMLSYLIDEERIKDMSPY
                     DAIRDFTMEYPTHIYDLALSNTQSRRSRAGKEFESILELLMMGAGIPVDVQGAIGKSFF
                     QKNQIGKLVDLVMPGVVQYTSNKRNTMLISAKTTLRERWQEVPEEVNRTGIREMYLATL
                     DDSFSEETINILYEANVVVVTTIENKNFKYKNNNRVLTFEDMLQSAMELSRKWNNVSYT
                     DSEKEEIQRSILKQIEKYSDFPYVVNYYRNRLSALFD"
                     /name="type II restriction endonuclease"
     Typing          854..939
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          1250..1348
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          complement(282..832)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.213"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaactaaaa ggatctcttg agatcctttt tttctgcgcg taatcttgga cctgtaaacg
       61 aaaaaaccac cctggcaggt ggttttttcg aaggttagct aatcctggca gattatctaa
      121 ccgaggtaat ctggcttcag cagagcacag ataccaaata ctgtccttcc agtgtagccg
      181 tagttaggcc atcacttcaa gaactctgta agcatctgga taaatctcgc tctgctaatc
      241 cggttaccag tggctgctgc cagtggcgtt aaggcgtgtc ttaccgggtt ggactcaaga
      301 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      361 agcttggagc gaacgaccta caccgaactg agataccaac agcgtgagct atgagaaagc
      421 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      481 ggagagcgca cgagggagcg accaggggga aacgcctggg atctttatag tcctgtcggg
      541 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      601 tggaaaaacg ccagcggcgc ggcctttttc cttggttttg cctttttgtt cacatgttct
      661 ttccggcctt atcccctgat tctgtggata accgcattac cgtctttgcg tgagctgata
      721 ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaagagc
      781 gcctgatgcg gcattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgct
      841 cttttcttat aatctgcact gatgccgcat agttaagcca gtatacactc cgctatcgct
      901 acatgactgg gtcatggctg cgccccgaca cccgctaaaa ccccctgacg cgccctgacg
      961 ggcttgtctg ctgccgaaaa cgctcacggg aaagccgtga ccgcctccgt cagcttcgtt
     1021 atgttcctgt ttcactttcg gcctcaaagc gaatctggat gctgttctgg agtttttctg
     1081 cgagttcgtg cagtcgttcg cacatggtcg cctgttcgtc ggcatccaga gcatccagtt
     1141 tttcaagtag tgtcaggctc tggcttttga taaatcgcgc catattcagc gcagtttgtt
     1201 gttgtttatt caccttccgt tgttctccgt cagttccggg tgtcgggggg accctgagca
     1261 ggtggtaatt gtaagacgtt gcgggcgacg gtattacaat tgcacatcct gtcctgtttc
     1321 tttaggacgt atcatggcac agcatatcaa ctgataaata atccctgtgc agtgcagccg
     1381 ctgttgcata aaatttcgct ctgcatcgag tgtttcttaa tcaataattc cgctgatact
     1441 ccggaaaaac gcgagtctgt tctcataccc gaaatgccca caccccacgc aaaaccaact
     1501 ttttgctgat ttgcttttta aatcatgttg ttatggtttt aaaaatatat tttcttattc
     1561 agatttatac aaaagcacgt cgtcgacgac cctatggcga cgctatggcg accctactta
     1621 gggtcagtac tgactatctg tagaaaaaac agttaattaa tgtaggtgcg cccctagggg
     1681 cgcgtcgcct ttttttacat aacgcccctg ggagcgctgc tagaggtgtc tgttcagata
     1741 tgtagcaatg agtcagggat tcacgaactg aaaatgtccg aaaatgtgtc tcatgggcac
     1801 catatgcgcc caaaccgatc gccattcact ttcatgcata gctatgcagt gagctgaaag
     1861 cgatcctgac gcgtttttcc ggtttacccc gggaaaaaac atctcttttt gcggtgttcg
     1921 cggcagaatc gcgttcagcg cgttttagcg gtgcgcgtaa tgcgacgtta tggtaaatcc
     1981 atatgggggg taattggatt ctgggccgca ttgtgtcctc atgacttagt ttatgaatct
     2041 aaggtcgata tgattttttc tgctattgca ttgataacag ggactgcgac tgaattccca
     2101 aattgcttat aagcctgtgt atccgataca gggataataa aatcactagg aaacccttga
     2161 agtcttgacg cttcccgcgg agttattttt cttgggttac ttcctttttg ctctattaat
     2221 atttcgctac catctttata gtacctcgca cttatggtat tagtatatgg gctattttca
     2281 ttaaataaac catacccaaa gcctttaccg gctgctgcat ttacaagttt cctgcgttgg
     2341 tgtccattcc agagcgcatc tgatagcgta tatttattgt ctacgctctt ttcaagaata
     2401 tctccaactc ttgtttttgt ttttaatggg gttggaaaag taaaatgttc atgatttctt
     2461 actttttctt tattaaagcc tacaatgtat atgcgctctc tattttgtgg aacaccaaag
     2521 tcttttgcag cgaagatatt gtaatacaca gtgtaattca attcttcaag tgtgtttttt
     2581 attattgaaa aagttctacc tttatcatgt cctagcaagt tttttacatt ttcaagtaaa
     2641 aaagcatgag gttttttttc ctttattatc cttgctatat caaaaaacaa agttcctctt
     2701 gtatcattaa atcctttttt gaggcctgct tgagagaatg ctacacaagg aaacccaccg
     2761 acaagaattt catgatcagg aatatctttt tcatcgattt ttgttatgtc cccatcaggg
     2821 aaatcaccat aatttgcatg atatgtcttt tgagcaaact tatcccattc gctggaaaaa
     2881 acaacattta cagcgttagt ttgatgaaat ccaagtctgg ttccaccgat tccagcaaat
     2941 aaatcgatca tgcgatatct gccattctcg ttgtttgggt aaggtggtgt gtctggaaag
     3001 tctataactg cttttagttc cgccccagta ggttttgttt ccccgcgctc ccatcttctt
     3061 atggttctgt ctccatattt tgaaagacca agggcgtctg caaactcttt ttgagtcata
     3121 tgaagtcttt ctcttttttc tttgattgtt gctgcgatat tatcagtcat gcatgtctac
     3181 cagaattaag tgttggtttt aggacaattt gtcctgtttt gattccaatt atgaacgtgc
     3241 atgaaaaaac tttcaagtag atataaaagg ttatttttta tgcaaagatt aagtcctggt
     3301 gaatttaaaa ctctaatttc taaagagaga aaatcgcatt ttattacgcc atttgctttg
     3361 gtttataaaa cattttgtga tttagggtat gatcaaaaaa atagtgatta ttttttgaat
     3421 aatccaagtg aatatataat tgcaatgaga aaaaactgtt ggaaagaatt tgaaccattt
     3481 gaaaaagaat tcacaacaag aatgttatca tatcttattg atgaagaaag aataaaagat
     3541 atgagtcctt atgatgccat tcgtgatttt acgatggaat accctacaca catatatgat
     3601 ttggcactgt ctaacactca atccagacgt agcagggcag gaaaagagtt tgagtctatt
     3661 cttgagctat taatgatggg ggcaggaatt cctgttgatg tgcagggggc tattgggaag
     3721 tcattttttc aaaaaaacca aataggtaaa cttgtagatc ttgttatgcc aggtgtagtc
     3781 cagtacacat ctaacaaaag aaatacaatg ttgatttccg ctaaaacaac gctgagggag
     3841 cgatggcaag aagtacctga agaggttaat agaacaggaa ttagagaaat gtatctagct
     3901 acactagatg attcttttag cgaggagaca ataaatatat tgtacgaggc taatgtggtc
     3961 gttgtaacga cgatagaaaa taaaaatttc aaatataaaa ataataatag ggtgctaacc
     4021 tttgaagata tgcttcagtc agcaatggaa ttatcaagaa agtggaataa tgtaagttac
     4081 acagactctg aaaaagagga aattcaacgg tcaatattaa aacaaataga aaaatatagt
     4141 gatttccctt atgttgttaa ttattataga aataggctta gtgctctttt tgactaa
//
