LOCUS       NC_017648               5163 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Escherichia coli O7:K1 str. CE10 plasmid pCE10B, complete sequence.
ACCESSION   NC_017648
VERSION     NC_017648.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02603406
            Assembly: GCF_000227625.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O7:K1 str. CE10
  ORGANISM  Escherichia coli O7:K1 str. CE10
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Lu,S., Zhang,X., Zhu,Y., Kim,K.S., Yang,J. and Jin,Q.
  TITLE     Complete genome sequence of the neonatal-meningitis-associated
            Escherichia coli strain CE10
  JOURNAL   J Bacteriol 193 (24), 7005 (2011)
   PUBMED   22123760
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Lu,S., Yang,J. and Jin,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2011) Microbial Genome Center of Chinese Ministry
            of Public Health, Institute of Pathogen Biology, CAMS&PUMC, 6
            Rongjing Eastern Street, BDA, Beijing 100176, P.R.China
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000227625.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 01:26:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,187
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 259
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 259
            Pseudo Genes (frameshifted)       :: 92 of 259
            Pseudo Genes (incomplete)         :: 152 of 259
            Pseudo Genes (internal stop)      :: 66 of 259
            Pseudo Genes (multiple problems)  :: 42 of 259
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003036.1.
            Bacteria/DNA are available from Dr. Kwang Sik Kim
            (kwangkim@jhmi.edu).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Escherichia coli O7:K1 str. CE10"
                     /mol_type="genomic DNA"
                     /strain="CE10"
                     /serotype="O7:K1:NM"
                     /isolation_source="cerebrospinal fluid of a neonate with
                     meningitis"
                     /host="Homo sapiens"
                     /db_xref="taxon:1072459"
                     /plasmid="pCE10B"
                     /geo_loc_name="USA"
                     /note="isolated in the 1970's"
     gene            1043..1957
                     /locus_tag="CE10_RS27170"
                     /old_locus_tag="CE10_B1"
     CDS             1043..1957
                     /locus_tag="CE10_RS27170"
                     /old_locus_tag="CE10_B1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271365.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(2161..2559)
                     /locus_tag="CE10_RS27175"
     CDS             complement(2161..2559)
                     /locus_tag="CE10_RS27175"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            2736..3662
                     /locus_tag="CE10_RS27180"
                     /old_locus_tag="CE10_B2"
     CDS             2736..3662
                     /locus_tag="CE10_RS27180"
                     /old_locus_tag="CE10_B2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            3665..4198
                     /locus_tag="CE10_RS27185"
                     /old_locus_tag="CE10_B3"
     CDS             3665..4198
                     /locus_tag="CE10_RS27185"
                     /old_locus_tag="CE10_B3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(4264..4473)
                     /locus_tag="CE10_RS27190"
     CDS             complement(4264..4473)
                     /locus_tag="CE10_RS27190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          1044..1957
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.017"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2737..3659
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gagtggaggt tgttacagtc gcttttataa aacagcaacc agtttttttc aatagtctga
       61 tgatggttgt gacagttttg tgatattgtt caaaatgttt cttgttgaat gtttgttttt
      121 tggtctattg gtgtctggtt ttattttttc tgctattaca tatcaagaaa tgtgattggt
      181 gtagcaagag tggaatgcga tgtggataaa ctaattggtt gtcgaatcta atttacttag
      241 aaaactacac ttgtttttgt gtatgaattt agtgaatttg tctgtctatg tgttttcgtg
      301 catttataaa tccttcgtca ggatcttaaa tttagtgttg aatatgtagg gcctgtacgg
      361 taattattat ttagcgtagc tacttcatcg ttgttcgaac acaaccaaca acctgtaatt
      421 gttgtttctc ttggttcccc acttttgtgg gtttttttat ctcttccgtg catcgtaaag
      481 cgcggactcc ttctggaaag gttgttttag ggcatcactg aggcattttt tgcgtttgtg
      541 aatattgagc atccgataac taaaatattt cccatagact ctgattcttg ccaccgaccc
      601 caggtcggtt tttttttgtc ctggcgagat gtgaagtttg gaaacaggaa accccgtcag
      661 agcgccacgc accaggggag aaaacttgtc gttttgagcc gttcggtgtt cagaacgcac
      721 gaaaccgatc gcacatcgct ttcgtgcata gttatgcaac gccctgaaaa cgattctgac
      781 gcgtttttct ggtttggcct ggtgttttct tgtctttttg cgttttttgc gccagaaagc
      841 gattgagggc gttttacatg gtacgtacaa cgggagttat ggtaaatgga tcggatttgc
      901 gggaaggctc gacaggtttt gtcgttgggg gtagtgtaag cgactgaaaa acaaacgccc
      961 ctgaaattcg tgtctatccg ccaagacttt cacgaaaata caggggcgat gtcggtcgat
     1021 aacctggaag gaatcgtaac acatgagcgc cgcgcttcaa tacttcgaag aaaatttacc
     1081 ccaccgtccc tatcacacgg atgatctcgc tttcggcctt cgtatctccg gcaaagggcg
     1141 cgcgcttctt gcacggtata tccagcagaa ccagcctcat gcacagttct ggctggtttt
     1201 tgacgttgac cgcgaggggg ctgcgattga ctggagcgac cggaacgctc ccgcgccgaa
     1261 catcaccgtt aaaaatcctg tgaacggaca cgctcacctg ctctacgcac tcaacatcgc
     1321 cgtgagaacc gcgcctgatg cctcggttaa ggcgctgaaa tatgccgcag cggttgaacg
     1381 ttcgctgtgt gagaaacttt gtgcggatgt gaactacagc ggcctgattt gcaaaaatcc
     1441 gttccacctg gaatggcagg tgatggagtg gcgcgaggac gcctataccc tcgatgaact
     1501 ggcagattat ctcgatttga gcgcctcaga gcgtcgtagc atcgataaac attacgggat
     1561 ggggcgaaac tgccacctgt tcgaaatgac gcgcaaatgg gcttacaggg cgattcgtca
     1621 gggctggcca gcgttctcac agtggcttga agcggtgatc cagcgtgtcg aaatgtacaa
     1681 tgcatcgctt cccgttccgc tttcactggc tgaatgtcgg gctattggca agagcattgc
     1741 gaaatacacg cacaggaact tcacgccgga aactttcgca cagtatgtgg ctgatacgca
     1801 cacgccagaa attcaggctg cacgcggtcg caagggcggt tctaagtcta agcgcagcac
     1861 agtagctaca tctgcacgca cgctgaaacc gtgggaggca ttagggatca gtcgcgcctg
     1921 gtattaccaa ctgaaaaaac gaggtctcgt agagtagacc aaataagcct atatcagata
     1981 acagcgcctt tctggcgtct ttttgagcag caggtttttt gccgtttttc cccgttcagc
     2041 cctgcaatgg cgcggctttc cgcgtgattg tggtggtagt gctcgccgca gtctcatgac
     2101 cgagcgtagc gagcgaatga gcgaggaagc accagggaat cggaacacgg atggaggcac
     2161 ttacgcgtct gggtttgggg atgtctccag atcgtactta agttccttaa gtgcttgtaa
     2221 tatcctgtct tttttatagc atagctcgcc tgtttttgtg tctaccagtc ctgcttcgat
     2281 cagtagccca gccgcgagaa agagccggtg attgcgttcc cgtcgttgtt tttttccctc
     2341 gattcgtttt tggaccgcct gttttcgttc gctctcctgt gcctgtaagt cgtttttttc
     2401 cgctgttgcc agtacgtgga gaagtttgcg ttcctgttct gtgagtccgg cttcgttgtc
     2461 gatcaggtca ataatctgtc tctgtcgtgc ggtctgttct ccgagcttcc tgatgaatcc
     2521 gatccgtttc ttcagccatt cgatgttgct gatgcgcatg ttcgtgatgc tccctggtgt
     2581 ttttttgcgg tgtgttgtgg tcgtgcagta tatcgcttgc ggtcggcggg tgcagggggt
     2641 atcttccgtg ctaattcgta aaattagaag gaagcgcact tatatgtttc tctccttcgt
     2701 cgagaaacag tgcgctcctg cagagggctg aaaccgtggc aacctatcac ctcagcgtta
     2761 aatttggtgg caaggggcag gctgcaaacc acgccgatta catcgagcgc aaagagaaat
     2821 accgcgaccg tcaggatttg gaatattccg cacacggaaa catgccggaa tgggccaggg
     2881 ataatccttc ccatttctgg caggcggctg accagtttga acgtgcgaac ggttcgacct
     2941 atagggaact tgaaattgca ttaccccgtg aactgacacc ggaacagcgc cttgaactgg
     3001 tgcaggattt tgtacgtcag gaagctggcg aacggcacgc ctggagtttt gccattcaca
     3061 atccgaaagc gagcattgac ggcggcgaac agcctcatgc gcatatcatg atgagtcagc
     3121 gtgtgaatga tggcattgaa cgtacaccgg aacagtattt ccggcgctat aacgccaggt
     3181 atcccgagcg gggaggtgcg aaaaaggatt ccggaagcct gactctcact cagcaaaaag
     3241 agcagttgcg ggaattgcga aaacgctggg aggtgaagca caacgaacat atgagaaagc
     3301 atggctttga aaggggcttc attgactgcc ggacgctgaa agaacagggc attgaacgac
     3361 gtcccgaagt gcatctgggt tttgaagctg ccggacggct tgatgatgcc cagcgtcatg
     3421 acatcatgca aaaacggtct gaaccggact attcccgcca tcttgaacag gcgcgaacgg
     3481 tcgggcatga catcagggaa tggctggaca ctccggttaa gcaacatgaa ctggtcagaa
     3541 ggctgcaaca tgatgttgcg cgggagcatg gctttgacat gagagacaga gtgagcagac
     3601 agaaccgaac tgcacctgaa cgggttaaat caccgtcacg tggattttct atgggattct
     3661 gaacatggcc agcacagaag aaaaattact cacgctcctg ttatcggtgg agcatctgca
     3721 acataccgca atggagcaac agaaagctgt tgctgattct ctggctgtgg caagggaaac
     3781 tcttcgcgga tatcgggata tttctgacgg tgtggcaaaa caggtcaggg atgaagtcag
     3841 aaatgaggtg cagaagctgg acgtggcagg actgataggt gagcgcatca gccaggattt
     3901 tcagcggctg gaagagtcag tcgcaaacat gaaggcaaac gcggatgccc tgaacaggga
     3961 tattgcgaca attcacagca atatcgtgca ggaatacaac acccttaaag ggtatagctg
     4021 gaaatggctg ggtggggtgt cagtggcttt tctggttatg ttctgtgtgc tcggatgggg
     4081 aatgaaaaca atactcgaca ggaactacaa cgccacagcg gcggtgtatc agaaagttgt
     4141 gacactggaa gataaactgg tggagaaacc tgtcacgggg aagagccgga aaaactgacg
     4201 cgcttcgctt gtccaacgcc tcacgacccg cgaaaggatt tcgcgaaccg ttcaccgttt
     4261 ttttcaatat gattctgtct gctttggtcg ataaagcaga cgatagtatt ccagccttcg
     4321 tatatataac ggtacgtgct catcagtaag agctggcggt attgtgttat cagggggaag
     4381 ttttttcaga tactcacgaa atgcctggct tgatgccata aaatctacag tagcatcgat
     4441 aatatgtggg gtgttttcac ctaattttgc catgatgata atccttatat tatcacggtt
     4501 tgtgaatgat agggtgtcac tattatttat gcgttagtga accagaaaaa atcccccgtt
     4561 ggcatagggg gatatgtaag gagaaataac ttcgcaggtg ttcttgtttg tataacgtga
     4621 aaagactgca cgcaacttaa taagtctgcc gtacttactt tcagttttca acataaggtt
     4681 tataacaatg tatggaatgg aagtgagctt taaggtttca gctctaaaaa ataaaatatt
     4741 tattagttgg ctggttgttg ttcttgttct gtatgtatat aaacactatc gacatattat
     4801 gaccgagaac attccttacc ccctctgcgc tcgcagaggt tttttttgtc attaattaat
     4861 aatgaactga aatgcagttc cgatacctcc atttgcacat tatccctgac atatggtacc
     4921 ggaaccgcat taattttatg ttataagagt ttatttttaa tgtccagatg gtttaatagt
     4981 atttatatgt aattagctgt atggatagta ctggttttat tgtataactt gtcgtttttt
     5041 ttattgtttt tgtgtggtgt gctaatcttt aagttataaa aaaccccttg gaatacaggg
     5101 gcaaaagtaa ggaaaacaaa tggatgctca gtttgtgtga cactttaaac taatggaact
     5161 ata
//
