LOCUS       NC_017339              20730 bp    DNA     circular CON 07-SEP-2023
DEFINITION  Staphylococcus aureus subsp. aureus JKD6159 plasmid pSaa6159,
            complete sequence.
ACCESSION   NC_017339
VERSION     NC_017339.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02604217
            Assembly: GCF_000144955.2
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus subsp. aureus JKD6159
  ORGANISM  Staphylococcus aureus subsp. aureus JKD6159
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20730)
  AUTHORS   Chua,K., Seemann,T., Harrison,P.F., Davies,J.K., Coutts,S.J.,
            Chen,H., Haring,V., Moore,R., Howden,B.P. and Stinear,T.P.
  TITLE     Complete genome sequence of Staphylococcus aureus strain JKD6159, a
            unique Australian clone of ST93-IV community methicillin-resistant
            Staphylococcus aureus
  JOURNAL   J Bacteriol 192 (20), 5556-5557 (2010)
   PUBMED   20729356
REFERENCE   2  (bases 1 to 20730)
  AUTHORS   Wick,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2022) Department of Infections Diseases, Monash
            University, 85 Commercial Road, Melbourne, VIC 3004, Australia
  REMARK    Nucleotide sequence updated by submitter
REFERENCE   3  (bases 1 to 20730)
  AUTHORS   Chua,K., Seemann,T., Harrison,P.F., Davies,J.K., Coutts,S.J.,
            Chen,H., Haring,V., Moore,R., Howden,B.P. and Stinear,T.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2010) Microbiology and Immunology, University of
            Melbourne, Royal Parade, Parkville, Victoria 3010, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUL-2022
            Assembly Method :: Trycycler+Medaka+Polypolish v. JUL-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 645.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/05/2023 05:05:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,810
            CDSs (total)                      :: 2,728
            Genes (coding)                    :: 2,644
            CDSs (with protein)               :: 2,644
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 58 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 22 of 84
            Pseudo Genes (multiple problems)  :: 20 of 84
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP002115.1.
            Bacteria and source DNA are available from Dr Benjamin Howden
            (benjamin.howden@austin.org.au).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20730
                     /organism="Staphylococcus aureus subsp. aureus JKD6159"
                     /mol_type="genomic DNA"
                     /strain="JKD6159"
                     /sub_species="aureus"
                     /db_xref="taxon:869816"
                     /plasmid="pSaa6159"
     gene            106..782
                     /locus_tag="SAA6159_RS13920"
                     /old_locus_tag="SAA6159_03860"
                     /pseudo=""
     CDS             106..782
                     /locus_tag="SAA6159_RS13920"
                     /old_locus_tag="SAA6159_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /name="replication initiator protein A"
     gene            <793..1075
                     /locus_tag="SAA6159_RS13925"
                     /old_locus_tag="SAA6159_03865"
                     /pseudo=""
     CDS             <793..1075
                     /locus_tag="SAA6159_RS13925"
                     /old_locus_tag="SAA6159_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            2286..2903
                     /gene="cadD"
                     /locus_tag="SAA6159_RS13930"
                     /old_locus_tag="SAA6159_03870"
                     /old_locus_tag="SAA6159_p02"
     ARG             2287..2900
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="99.51"
                     /coverage="100.0"
     gene            2922..3269
                     /locus_tag="SAA6159_RS13935"
                     /old_locus_tag="SAA6159_03875"
                     /old_locus_tag="SAA6159_p03"
     CDS             2922..3269
                     /locus_tag="SAA6159_RS13935"
                     /old_locus_tag="SAA6159_03875"
                     /old_locus_tag="SAA6159_p03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119691.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNQEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            3438..3530
                     /locus_tag="SAA6159_RS13940"
                     /old_locus_tag="SAA6159_03880"
     CDS             3438..3530
                     /locus_tag="SAA6159_RS13940"
                     /old_locus_tag="SAA6159_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            3742..4716
                     /locus_tag="SAA6159_RS13945"
                     /old_locus_tag="SAA6159_03885"
                     /old_locus_tag="SAA6159_p04"
     CDS             3742..4716
                     /locus_tag="SAA6159_RS13945"
                     /old_locus_tag="SAA6159_03885"
                     /old_locus_tag="SAA6159_p04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="WP_000072391.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFNS
                     SSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFEMN
                     KASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLMQSVPSEIKENSGWI
                     RVQDCGKNVKKLKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIEDIEN
                     YLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYEEDLKL
                     LQKNYKTTLVDIKGLNKHFYKECDRCLKAYNIFIEGINKHLYE"
                     /name="DUF4062 domain-containing protein"
     gene            complement(4836..5651)
                     /locus_tag="SAA6159_RS13950"
                     /old_locus_tag="SAA6159_03890"
                     /old_locus_tag="SAA6159_p05"
     CDS             complement(4836..5651)
                     /locus_tag="SAA6159_RS13950"
                     /old_locus_tag="SAA6159_03890"
                     /old_locus_tag="SAA6159_p05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000164958.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSSR
                     YYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAIIDL
                     GQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMIFIG
                     MPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDYEAIT
                     TIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
                     /name="AAA family ATPase"
     gene            complement(5644..7087)
                     /locus_tag="SAA6159_RS13955"
                     /old_locus_tag="SAA6159_03895"
                     /pseudo=""
     CDS             complement(5644..7087)
                     /locus_tag="SAA6159_RS13955"
                     /old_locus_tag="SAA6159_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /name="DDE-type integrase/transposase/recombinase"
     gene            complement(7059..7652)
                     /locus_tag="SAA6159_RS13960"
                     /old_locus_tag="SAA6159_03900"
                     /old_locus_tag="SAA6159_p08"
     CDS             complement(7059..7652)
                     /locus_tag="SAA6159_RS13960"
                     /old_locus_tag="SAA6159_03900"
                     /old_locus_tag="SAA6159_p08"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690638.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNGTPI
                     KTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
                     /name="recombinase family protein"
     gene            complement(7916..8296)
                     /gene="blaI"
                     /locus_tag="SAA6159_RS13965"
                     /old_locus_tag="SAA6159_03905"
                     /old_locus_tag="SAA6159_p09"
     ARG             complement(7920..8296)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.21"
                     /coverage="100.0"
     gene            complement(8286..10043)
                     /gene="blaR1"
                     /locus_tag="SAA6159_RS13970"
                     /old_locus_tag="SAA6159_03910"
                     /old_locus_tag="SAA6159_p10"
     ARG             complement(8290..10043)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.56"
                     /coverage="100.0"
     gene            10150..10995
                     /gene="blaZ"
                     /locus_tag="SAA6159_RS13975"
                     /old_locus_tag="SAA6159_03915"
                     /old_locus_tag="SAA6159_p11"
     ARG             10151..10992
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            11364..11972
                     /locus_tag="SAA6159_RS13980"
                     /old_locus_tag="SAA6159_03920"
                     /old_locus_tag="SAA6159_p13"
     CDS             11364..11972
                     /locus_tag="SAA6159_RS13980"
                     /old_locus_tag="SAA6159_03920"
                     /old_locus_tag="SAA6159_p13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436987.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001566953.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRPV
                     LQEALNFLRMGDRFVVESIDCLGRNYDEVIHTVNYLKDKEVQLMITSLPMMNEVVGNPL
                     LDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRI
                     IYHRVVEMLEEGQSISKIAKEVNITRQTIYRIKNDNDLI"
                     /name="recombinase family protein"
     gene            12386..12679
                     /locus_tag="SAA6159_RS13985"
                     /old_locus_tag="SAA6159_03925"
                     /old_locus_tag="SAA6159_p14"
     CDS             12386..12679
                     /locus_tag="SAA6159_RS13985"
                     /old_locus_tag="SAA6159_03925"
                     /old_locus_tag="SAA6159_p14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000726502.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGKH
                     YVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
                     /name="lactococcin 972 family bacteriocin"
     gene            12754..14688
                     /locus_tag="SAA6159_RS13990"
                     /old_locus_tag="SAA6159_03930"
                     /old_locus_tag="SAA6159_p15"
     CDS             12754..14688
                     /locus_tag="SAA6159_RS13990"
                     /old_locus_tag="SAA6159_03930"
                     /old_locus_tag="SAA6159_p15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000713729.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNWD
                     KSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLTPS
                     DLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLVPFI
                     FLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFIANIL
                     IIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKGYKFSI
                     FMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNIRNSEEK
                     QILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNFYKNINHD
                     FKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSKNVSVFSFD
                     RTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLKDNHLEEEIS
                     GITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWNKKQLLLRKIH
                     GYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHLLQIAYIKYLEK
                     YFKDLMREI"
                     /name="bacteriocin-associated integral membrane family
                     protein"
     gene            14692..15003
                     /locus_tag="SAA6159_RS13995"
                     /old_locus_tag="SAA6159_03935"
                     /old_locus_tag="SAA6159_p16"
     CDS             14692..15003
                     /locus_tag="SAA6159_RS13995"
                     /old_locus_tag="SAA6159_03935"
                     /old_locus_tag="SAA6159_p16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="WP_000831610.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVPK
                     DTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFLK"
                     /name="YxeA family protein"
     gene            15015..15641
                     /locus_tag="SAA6159_RS14000"
                     /old_locus_tag="SAA6159_03940"
                     /old_locus_tag="SAA6159_p17"
     CDS             15015..15641
                     /locus_tag="SAA6159_RS14000"
                     /old_locus_tag="SAA6159_03940"
                     /old_locus_tag="SAA6159_p17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000593103.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLLN
                     IIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFKKN
                     KKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTGSLD
                     TKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
                     /name="ABC transporter ATP-binding protein"
     gene            15879..16508
                     /gene="mobV"
                     /locus_tag="SAA6159_RS14005"
                     /old_locus_tag="SAA6159_03945"
                     /pseudo=""
     CDS             15879..16508
                     /gene="mobV"
                     /locus_tag="SAA6159_RS14005"
                     /old_locus_tag="SAA6159_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013258.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            16602..17147
                     /locus_tag="SAA6159_RS14010"
                     /old_locus_tag="SAA6159_03950"
                     /old_locus_tag="SAA6159_p20"
     CDS             16602..17147
                     /locus_tag="SAA6159_RS14010"
                     /old_locus_tag="SAA6159_03950"
                     /old_locus_tag="SAA6159_p20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000273005.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTDFEKYTKQIKAAQMILD
                     DYECIKSGKSLKKAEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKDKE
                     LNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKKKYQ
                     AQEREIIFKEDREDGYTL"
                     /name="hypothetical protein"
     gene            17531..18391
                     /locus_tag="SAA6159_RS14015"
                     /old_locus_tag="SAA6159_03955"
                     /old_locus_tag="SAA6159_p22"
     CDS             17531..18391
                     /locus_tag="SAA6159_RS14015"
                     /old_locus_tag="SAA6159_03955"
                     /old_locus_tag="SAA6159_p22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_000145511.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLRL
                     SFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIHKR
                     EQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLKIHI
                     DDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEFTFDA
                     EKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKEREDFLKQL
                     ELNWE"
                     /name="replication initiation protein"
     gene            18478..18819
                     /locus_tag="SAA6159_RS14020"
                     /old_locus_tag="SAA6159_03960"
                     /old_locus_tag="SAA6159_p23"
     CDS             18478..18819
                     /locus_tag="SAA6159_RS14020"
                     /old_locus_tag="SAA6159_03960"
                     /old_locus_tag="SAA6159_p23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000761341.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSLD
                     ILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAGFL
                     VWAIHSFMG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(19058..19129)
                     /locus_tag="SAA6159_RS14025"
                     /old_locus_tag="SAA6159_03965"
     CDS             complement(19058..19129)
                     /locus_tag="SAA6159_RS14025"
                     /old_locus_tag="SAA6159_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106873653.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            19278..19463
                     /locus_tag="SAA6159_RS14030"
                     /old_locus_tag="SAA6159_03970"
                     /old_locus_tag="SAA6159_p24"
     CDS             19278..19463
                     /locus_tag="SAA6159_RS14030"
                     /old_locus_tag="SAA6159_03970"
                     /old_locus_tag="SAA6159_p24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000466619.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHII
                     RVKDGLLGWRKEIVTE"
                     /name="hypothetical protein"
     gene            complement(19682..20425)
                     /locus_tag="SAA6159_RS14035"
                     /old_locus_tag="SAA6159_03975"
                     /old_locus_tag="SAA6159_p25"
     CDS             complement(19682..20425)
                     /locus_tag="SAA6159_RS14035"
                     /old_locus_tag="SAA6159_03975"
                     /old_locus_tag="SAA6159_p25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843758.1"
                     /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNAR
                     QNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSANPND
                     NDSDIEEKSEETEPKKGFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     ARG             10151..10995
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     BGC             7386..17679
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1133..1280
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.333"
                     /coverage="100.0"
                     /evalue="2.96e-25"
     Typing          17532..18391
                     /name="rep_cluster_2214"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(19683..20425)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          15880..16154
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.565"
                     /coverage="100.0"
                     /evalue="4.71e-46"
ORIGIN
        1 atgaataata tcttagtgca agcacctaga ttattaaata aacacaaaaa atgtaagcgt
       61 tttattacct cttaccaaac taaaattcgg aaagaggttt ttattatgtc aattaaactt
      121 tacaacataa atgaggaata cagagaaaag ttttatcaaa taccgaaagt attttttaca
      181 aatcctaaat atatcaagtt atccaatgat gcaaaaatgg catggggtat tcttagagat
      241 cgtttagatt tatcaattaa aaatggttgg gttgattcta aaacaggtaa tatttatttt
      301 tattacaaaa atgaaaaccc acaaaatata ttaaattgta aaaaagataa agtaattaaa
      361 ataaagaaag aacttaattg aagccgaatt actactacaa gaaagacaag gtttaaataa
      421 gacaaataaa ctttatttag ttaaaccaga agtaacagaa gatgatattt ataaaattca
      481 aaagcaagaa aacactgttg agagcagcaa tggcaaggaa gtcggaaaaa ccgacttcaa
      541 gaagtcgata aaacagaatc taatgatact gaacttagct atactgaatc taacaatact
      601 gaaataaatg atatgaatga cttgaatgat atagagtccg aaaatgaaat tagtaatcat
      661 tcaagtcaat gcaaggttat ttaatgcaaa caattttaac tgaatttgaa gaaacacatg
      721 cactttatat gcgccgtaaa aacatgaaac aatataatat ctttaatcaa tcattcaaat
      781 gattttataa caatcaaaga tacatcaaaa tatttcgtta aatcatctga ctttttaact
      841 gatagcgatg aaaaaatcaa gaaattgtca gtgatctaga aaaagatttg tatcgaaaac
      901 gtatgttaag ttatggtgga ttgcttaaat aaaaacataa aattttaaac ttagacgatg
      961 ctgaagatgg caatttaatt aatacaagtg atgaagataa aatagcaaat gaagaagaaa
     1021 aggcacattc gattacggca attttgaaaa gcaaaattat tatttaaggc attagtgata
     1081 actgatgtct ttttttatta cttttttgta aaaaaagtgc tgtcttattt ttgtgacaaa
     1141 tgctgtatgt agtgtcacaa aagtgtgaca cttgagcttt ttatgacccc acacaaaaac
     1201 attttcgaag ccttgaaata tctggctttg ccagatcccc cattcattga tggggacaaa
     1261 tttcccttat gctcttacgg agattttaga gttaaattaa aatttctaag tttttgataa
     1321 gaaaagccct gcaggaattt agaagaacgt ggggaagttt taaagaattt tatgctaatt
     1381 tttaatttgc atgtaaatgg gcagtgtcta aaaaatagtt attgtttttg ctcaaatttt
     1441 tgtttttgaa aatagcatat atttatttgg ctcatatttg cgttttaaaa gcgtatataa
     1501 gttttagata taaaactata tgaatcaccc ctaaatcttt aaaatgccct cttaaaattc
     1561 aaaataaggg cataaaaaat aaaagccccc ccagataaag tgaggtagct ttttctttcg
     1621 acgccgtatc gtcgatgtaa ttcaaaaaag ttatgggcta taaatctaca tcactttttc
     1681 aagaatgtag tagcaatatt caacgcaaat taatttttat aactgctcgg aatatctcaa
     1741 gccgtttctt taattttgaa ataaaaaaat cgacgaaggt cgattacgtt tttgcacatg
     1801 tcgatttatc cgacgtataa gtgcgccctt acgggattta actagattat aacgacgaat
     1861 tttagacctg taaagcaata acaattaggt ctaccaggta gataaagtgt acaaagtaga
     1921 accgaaaaag ttatttattt tgaagatttt tcgtagatat aatttgctaa taaacttcct
     1981 aaaactgaat aaagaaaaat agttaaactc atgtaatcac tccataattc tgaagtgtct
     2041 ccatgtctac gcatagacac gcatttatgc cgagaaaatt tattgtgcgt tgagaagaac
     2101 ccttaactaa acttgcagac aaatgtcggc atagcgtgag ctattaagcc gaccattcga
     2161 caagttttgg gattgttaag ggttccgagg ctcaacgtca ataaagcaat tggaataaag
     2221 cacctatgat tatttaaaaa tataagttat aatacattca aaaatatttt tgaatgaggt
     2281 gtattatgat tcaaacggtt gttgctgctg ctgttcttta tattgctaca gcagttgatt
     2341 tattagtaat tttattaata ttttttgcta gagcaaagac tagaaaagaa tatagaaata
     2401 tttatgttgg tcaatattta gggtctatta ttttaatatt agttagtttg tttttagctt
     2461 ttgtattaaa ttatgttcca gaaaagtgga tattaggttt attgggttta ataccaattt
     2521 atcttggaat taaagtggct atttatgatg attgtgaagg agaaaagaga gctaaaaaag
     2581 aattgaatga aaaaggattg tctaaattag ttggtacggt tgcaattgtt acgatagcaa
     2641 gttgtggcgc cgataatatt ggtttatttg ttccgtattt tgtgacatta agtgttacta
     2701 atttattact tactctgttt gtctttttaa ttttaatttt cttcttggtg tttactgcac
     2761 aaaaattagc taatatccca ggaattggag aaattgttga gaaatttagt cgttggatta
     2821 tggctatcat ttatatagct ttaggtttat ttattattat tgaaaatgac actattcaaa
     2881 caattttagg atttatattt taatttaggg tgtgatttta tatgagttat gaaaatactt
     2941 gtgatgtgat ctgtgtacat gaggataaag ttaacaatgc tttaagtttt ttagaagatg
     3001 ataaatctaa gaaattactt aacattttag aaaaaatttg tgatgagaag aaattgaaaa
     3061 tcatattatc tttgattaaa gaagatgagt tgtgtgtttg tgatatttct ttgatattga
     3121 aaatgagtgt tgcttcaact tcacatcatt taaggctttt atataaaaat gaggtacttg
     3181 atttttataa agatggaaag atggcatatt attttattaa agacgatgaa ataagagagt
     3241 ttttctctaa aaatcaggag ggtttttgaa acgagtgaaa cgagtttctt cttgtcttga
     3301 tactataaaa aacaaagtta tataacataa gacgacttat cggaagttat ctatcatcgt
     3361 atattcggag atgatataat atacaggtcg cctatctctc aggcgtcaat tcagacgcag
     3421 agaggaggtg cattactttg ctaatcattt tcgttcacat catagcacca gtcatcagtg
     3481 gctgtgctgt tgcgtattat acttattggc ttagtaaacg caacaaataa ttaataggcg
     3541 actttataag ccagcatcga aaaaatcccc acactattgc agtagtgtgg ggattggtgc
     3601 attaattgtt aatcattttc gttatctaga ttataacact ggttagtaga ggaatgcaaa
     3661 aagataatat tttaagtagt ctttttggat attatcatat gatatattta tgtaaattga
     3721 ttttttatta aaggagcata tatgagtaat actaaaatta aatatcaaat ttttatttca
     3781 tcaacttatt tagatttaat agaagagcgt caagcagtag tagaagcagt tttaaacagt
     3841 aataatattc cagcaggtat ggaacttttt aactcaagta gtaaaagcca atgggaaata
     3901 ataacacaat ggatcaatga atcagatgca gtgatattat tgttaggttc tagttatgga
     3961 acaattgata gtgaagaaaa tattagctat acagaaatgg agtacaattt tgctaaatct
     4021 ttaggtaaac ctatatatgt ttttgagatg aataaagcat ctaccgaatc cgcggaaaag
     4081 tctaaaaaat tagatgagtt taagacaaaa gttaaagaaa atagactaat tagtccaaat
     4141 attaatgata tttcggaatt aaaaagtaaa gtatctcaga ctttaatgca aagtgtacct
     4201 agtgaaatta aagaaaatag tggatggata agagtacagg attgtggtaa aaatgtaaaa
     4261 aaattaaaaa aattggatgc tatacaaaga gatttagata catatgattt tttgaaagaa
     4321 gaactttata aaatattcat gtatgtaaaa actttttatt tccagcacac accatttgat
     4381 gatagttata ttgaagatat agaaaactac ctgctaaatt atgaaaataa tccacataaa
     4441 tcattcatga aaataaaagt ttatgattat aaaaactttt taaaacattt aaaaaacata
     4501 ttgattttaa ttaaacaagg aaaaaagcca ttagctcaag ccggtcattt taaattaagc
     4561 ttttacgaag aggatttaaa attgttacaa aaaaattata agactacgct agtcgatatt
     4621 aaaggattga ataaacattt ttataaggaa tgtgataggt gcttaaaagc atataatatt
     4681 tttattgagg gtataaataa acatttatat gaatgatata agtacaccct aatcatacat
     4741 atataattgt tgtcacttta ctcgatatat gagaatgact ttacctaata aatgagaaaa
     4801 gcaacgcact atattaaaat gcgttgcttt ttctattatc gaatacctat aactaaacta
     4861 tctctagctg tttctacaac ttctgtactt attttatcta aaccatttat atccataatt
     4921 ctgtctatct gcgcaaataa acgatgaatc agcctaaaat tcccttttgt aattttaata
     4981 atcgtcgtta ttgcctcata atcagtaaag tcttcgagtt ttaaatcaaa tcctaagtct
     5041 tgccacttat attctaatat atgatgcgtc tcatctttac ttagattatc aaattcatgc
     5101 gcaaaaccta tacgcgaata tagttgagga taacgagata gttttttctc tatacccggc
     5161 ataccaataa atatcattgc taaattatgt tcatcataaa tagctcttaa ttgctctaaa
     5221 tgttgcactt taaggcgatc aatctcatct actataatta aatcaatata tttaaacata
     5281 tcactataat gtttactatg ttcatgtcca tacttattta caacatgtaa tttttgaccc
     5341 aaatcaatga ttgcagtaat actatataaa tcattcctta atttagtttg tttttctgct
     5401 ggtgcattat aaaatatttt atccaccgat agtatgtcat cggtcgtttt actagctaaa
     5461 tcttcccaac ctctatggtt aacttgtttt tcaatagtat cccaatttgt gtaataccta
     5521 gacgataaag tttttccgac accaggttga ccataacata taccaatata ctgatattta
     5581 atacaagcat cacaaaattc agcaaagcgt ttatactctt tcgtttcaat gaaattttga
     5641 tttttagtca ttacgatatc tctttaattt tttgacgttt ttagtcgttt cttgaggtga
     5701 ataaccacat tttttttctt ccttaattaa ttccgtggta cttggactag caatattttg
     5761 ttttaagtgt tttcttctct gactacgagc gtgctgtatg tcttttatat ctatggagta
     5821 atcagctatt tctggacata tagcagtaca aagaaattca tctctataaa acacacgtat
     5881 ttcagccata tcatttgggt tataacgaat cagtacatat tcacctacat aagcttctag
     5941 attagtatta ctgtatcgaa aaccttgaaa atgaatgcca tctgaatgaa cttttctaga
     6001 tttaggtatt tccaacaata ataaatcaag ttgttctaag ctacttggca tattagggaa
     6061 aaagtgatta ctattccaat gactgatagg cgtggtatga atagtactat gttctttttg
     6121 attataatct tcaattaaaa agtaacgtaa tttttcttca aaattgtgaa aatttattag
     6181 attactagga gtatcattat tgtttatata tccaggcagt tgttctaaga aagtttgatt
     6241 aactgtctga aagaatcgtt ctatttttcc acgaccacga ggaacaccaa ctttcgaaaa
     6301 catcaagtta attttcaaat caatagcgac ttgttccata tgatgtgaag taaagtcact
     6361 tccatgatct gtgtagaatt tttcgggtat accacacact ggccaattgg tatcattttt
     6421 attccatatt gcttggtgta gtgttaatgc tgtgttttga gcattggggg gcatcaaaac
     6481 ttaagaaata gcctgcaatt gcacgactat agtcatccat aataatagtt agccatggtc
     6541 tattgatatt acctttttgg tctaatataa agatatctag caaagtatgg tctgcttgcc
     6601 atatttcatt gggtctcgag gattctctaa tttgtattaa atcgtactta ttttgatagt
     6661 atttttcacc ctgatgagag aaatcaataa cagacttagg catcgcttta ataacactat
     6721 aaatttgttt ataacttggt atatcaaaat tattttcttt acagtaatta gtgattttcc
     6781 tatgaattgt ggctatacta tttcttttat tcataagcgc aaggcgttca atttcatcaa
     6841 ttatctttgg ttctatttta cgtgttcctt tatcagctct tgtcgcatag attagtcctt
     6901 taagtccttg ttctttatat gacttattcc acctataaag ggtgcttaac gcaatccctt
     6961 tacttttaga aatacttgat aaagattgtt cgcctaggat aaaaggtttt ataatattgt
     7021 acttttgagt agctttttta cgtttggctt cagaaaaatt agttagatac ttctccttgt
     7081 ttttcatctt ccttgtcctc caatttattg aggtaacgat aaattgttgt acgtgaaaca
     7141 tgccactgtt ctgcaattgt cttaattggc gtgccattat cataaagggt cttaagtaag
     7201 tttaaatctt tttgatttaa tttttctggt ctgcctccat atcggcctct agcacgtgct
     7261 gcaattcggc cagctgaaga acgctctaaa attaaattac gttcaaattc tgcaaatgct
     7321 gcgaataaat gaaataataa ttgtccagtt gaacttgatt tatccattgt tatattttct
     7381 tctaaactat ggaaacttac acctcgttcg ttaagctcat taactaatgt gattaaatcc
     7441 tccatattac gtcccaatct atctaatctc caaacaacaa tcgtatctcc tgacctcgca
     7501 aattcaattg ctttatctaa accaggtctt ttactttttg aaccactcat atggtcatta
     7561 aatatctttt cacaaccata tgtatttaat cgatcctcct gtaaatttaa attttgtaat
     7621 ccagttgaaa ctcttgcata acctattttc aaaatacaaa cctcctatat acatcttcaa
     7681 tgtaccataa cttaattaca aggtctttta tgaacataga ttttttgttg ggtttttgaa
     7741 cacataatcc tatgtaattc ttacttattt agacataaaa gaagagtgta cagaaaatgg
     7801 ttattttctg tacactctca tctttaataa tatattttaa atgtcattaa caaaactaat
     7861 ttaataagag tcaagcatag tttacatatg tattatgttt gaaacatacc tctaattact
     7921 ttttactaat atcatttaaa atatctcgca attcttcaat ttctttgtta tttaattctt
     7981 catttttcgc aaaattcaac actaaacttt tcatatcccc tccatacagt ttattaagaa
     8041 aggttttagc agttttcatt ttaatatcgt cttctttaat atttgatgag taaaaataaa
     8101 tattttctga tttgtatcgt tttataatct cttttttata tagtcttgtg attaatgttc
     8161 taatcgtttt atcactaact tctttatatt tttgaatttc aactacaatt tcattagctg
     8221 atactgattt tttatcccat attatattca taacatccca ttcagccata gatatttcaa
     8281 cttgcttatt ggccatttaa aacacccatt ccttttaata ttttttcact tattaattca
     8341 gcatttttcc cagacgggtt tccatctgat aaatgtgtag caaaataata cttatcatga
     8401 tttgtaatta cgtaacctac aaaccaccca ttattatact tcccgtttac tatacctgta
     8461 cctgttttcc catacagttc atacttttca tttttcttaa tcaataatga agaagataat
     8521 tgattctttg cttttttact aaaatggtta ttttgttcca tcatattttt aaaaactatt
     8581 acttgttcaa gattagatat tttcaaacta tcttccatcc aatagctttt ataacttccc
     8641 aaattttcat taccataatt taattgcttg agttgagccg cagtatagtt ctttggtatt
     8701 tgattactaa tacgttcgaa gtaccaatta actgaatttt gcattgctgt atttaaatct
     8761 tgttccttat tccaagactc gaaaagataa tgtttatgat tccatgacat acgtgaattt
     8821 ttatcactta taatatgacg gtcaagtcca aacagtgcta aataaatttt gtaagttgaa
     8881 tcaggagaat atctttttct actttctttt tcattataaa tatagtattt gtctttcttc
     8941 atgctgtaca taacgaaaga tccactattt gaaccaaaat ttttactttc atctaaaatt
     9001 tggtaatcgc tttgtaaggg ctttttataa ttataatcag ttaaggattg ccccattaaa
     9061 aactggcttt gaattatcat gataaaaaaa gtaaaaatac aaataaaaat tagaattaat
     9121 tttgactgct ttttcagatc ggcttctttt atattaatta atctattttt gagtaatgat
     9181 tttttaccat taaatgaatg gcttaatata tttttgttga aaaatgaggt ttttaatacg
     9241 gagtccataa tagattccgc ataagtttta aattcatttt tgttaatatt attaaggaca
     9301 aatctatcgg cttctacttc attgtcatga attatctttc tttttacaat atgtactaat
     9361 ggattgtaac tcataataat agagaagata ttaaagataa ttaaatgtaa agtatctcta
     9421 tttttagcat gagcatattc atgcagtatt atatatttta gtcttttatc aattacactt
     9481 ttaaaatatg aactaggaat caaaataata tatttcccat accaaaaagt tattggagat
     9541 tgaatagcct ctgcttttcg aatcacaata ttttttttat attgatggtt gaaaagtatc
     9601 gtatctattt tatttttttc attttcgttt agataaagtg actgtttctt taaatattta
     9661 agatataata aggctttcaa aaatttaaaa cttaaaataa taactaaaac tatccaaata
     9721 actgtgcaga tattatcaat tgaatcccaa ttaaacttat ggatatctgt tgcgaactct
     9781 tgaacaggtt tggtggtaat tatgttatgg ttcaagtcgt gtgacttact ttcaactgtg
     9841 ggcgcttgat tattcacatt attaaatttt ataaaagaga atttaattgg gataaaagga
     9901 attaatccag caagcacagt tagataccaa actttataat ttaaactata attaaaatag
     9961 cgttttaata tgtacctaaa aaatactaac aatagaaata taaaacaaaa gcttactaca
    10021 ctcattatta gtaatttagc cattttgaca ccttctttca aatatttata ataaacaatt
    10081 gacatcaata ttacaattgt aatattattg ttttgtaaaa attacaactg taatatcgga
    10141 gggtttattt tgaaaaagtt aatattttta attgtaatgg ctttagtttt aagtgcatgt
    10201 aattcaaaca gttcacatgc caaagagtta aatgatttag aaaaaaaata taatgctcat
    10261 attggtgttt atgctttaga tactaaaagt ggtaaggaag taaaatttaa ttcagataag
    10321 agatttgcct atgcttcgac ttcaaaagcg ataaatagtg ctattttgtt agaacaagta
    10381 ccttataata agttaaataa aaaagtacat attaacaaag atgatatagt tgcttattct
    10441 cctattttag aaaaatatgt aggaaaagac atcactttaa aagaacttat tgaggcttca
    10501 atgacatata gtgataatac agcaaacaat aaaattataa aagaaatcgg tggaatcaaa
    10561 aaagttaaac aacgtctaaa agaactagga gataaagtaa caaatccagt tagatatgag
    10621 atagaattaa attactattc gccaaagagc aaaaaagata cttcaacgcc tgctgctttc
    10681 ggcaagactt taaataaact tatcgcaaat ggaaaattaa gcaaaaaaaa taaaaatttc
    10741 ttacttgatt taatgtttaa taataaaaac ggagacactt taattaaaga tggtgttcca
    10801 aaagactata aggttgctga taaaagtggt caagcaataa catatgcttc tagaaatgat
    10861 gttgcttttg tttatcctaa gggccaatct gaacctattg ttttagtcat ttttacgaat
    10921 aaagacaata aaagtgataa gccaaatgat aagttgataa gtgaaaccgc caagagtgta
    10981 atgaaggaat tttaatattc taaatgcata ataaatactg ataacatctt atagtttgta
    11041 ttatattttc tattatcgtt gacatgtata attttgatat caaaacctga ttttcccttt
    11101 attattttcg agatttattt tcttaattct ctttaacaaa ctagaaatat tgtatataca
    11161 aaaaatcata aataatagat gaatagttta attataggtg ttcatcaatc gaaaaagcaa
    11221 cgtatcttat ttaaagtgcg ttgctttttt ctcatttata aggttaaata attctcatat
    11281 atcaagcaaa gtgacataat tgttgaattt atagtaactg aaacgtatga ttagggtgta
    11341 tattaattat gagatggaaa tcaatgattg ttggctatgc gagagtatca tcgaacgatc
    11401 aaaatttaga aagacaactt gaaaatttaa agacgttttg tgctgaaaaa atatttacag
    11461 aaaaacagtc tgggaaatct attgaaaata gaccagtact tcaagaagca cttaactttt
    11521 tgaggatggg agatcggttt gtagtagaat ccattgattg tttaggtcgt aattatgatg
    11581 aagtgattca taccgttaat tatttaaaag acaaagaagt acaattaatg attactagct
    11641 tacctatgat gaatgaagtg gttggcaatc cattactgga taagtttatg aaagatttaa
    11701 ttattcaaat attagcaatg gtttcagaac aagagagaaa tgaaagtaaa cgtcgacaag
    11761 cgcaaggaat tcaagttgcg aaggaaaaag gcgtatataa agggcgcccc ttactttatt
    11821 caccaaatgc taaagatcca cagaaacgta ttatttatca tcgcgttgta gaaatgttag
    11881 aagaaggcca atcaattagt aaaattgcga aagaagtaaa tataacaagg cagacgattt
    11941 acagaattaa aaacgataat gatttgattt aataaacttt gtttcaatgc aactgagagc
    12001 ttctcacagc gtctcaaaac agatgattat aatctattat gagtttttaa taataagaca
    12061 agaaaagaga gcgtaaatat gctctctctt tttctcatta acgaatgttt ataacgcaat
    12121 catgatgaga gtattaatac agttaattat ttgaaatact ttagtttata taatgttgaa
    12181 ttataggtag tgaaattgta tttattatac attgtcatat cgttgtttaa tgatagttaa
    12241 tttaaggata atagaattaa tattatatat agatgaacaa aacaagcaac agagcagaaa
    12301 aaacactttt agattatttt tactcattct ctattttatt tatttttgct ataataaata
    12361 aagaatatta aatggaggtg tttttatgaa aaagaaattt gtttctagtt gtattgctag
    12421 tacaatctta ttcggcactt tattaggtgt aacttataaa gctgaagctg caacagtaca
    12481 tgtagctggt ggtgtttgga gccatggtat tggaaaacat tacgtatggt cttattatag
    12541 tcacaataaa agaaatcatg gttcaacagc agtagggaaa tattcatctt ttagtggtgt
    12601 tgctcgacct ggtgttcaat ctaaggcatc tgctccaaag gcttggggtg gaaacaaaac
    12661 attttatagt cttcactaat tacatatatt aattaaggag aatagacaga attaagtaaa
    12721 cttaattctg tctattctct tgaaagaggc aatatgaaaa aattcaaatt atttttagat
    12781 tttagtactt tattattaat ttcagggcta ttatttttat ttttcttcaa agaaaatgaa
    12841 gaaattatac ctgaaagttc caatatttta acaatctcta attgggataa atctaacagt
    12901 aaatcaaaag tgttagatgt cattgaatca ggcgccaaaa atcaaaatat acaaataatt
    12961 aaatccgtta aagattttga taataaaaaa gaattttttg ttttcaactc aaaacgaaat
    13021 aattctgact ttatacgtaa taaaacatca cttttaactc catcagattt attaaacaga
    13081 gagataaaag gaaagtatta tattattggt gaacatttca atgtagaaga gcttacgagt
    13141 gaattaaaaa aagctggttt aacatctgaa gttgaataca taaatagaaa catattattt
    13201 tttgaattag tcatagataa tgatttatta gtgcctttca tattcttagg cgtattatat
    13261 cttttgtact tcttatatga cagaggatat aatttgaaat tttatgctat tcaaaagcta
    13321 catggctttt caactacacg cattattttt cataatattc atataaaaat catttattgg
    13381 ctagtactta ccgctatttt ctttattgct aatatattaa tcattcatat agccaattta
    13441 gaattggatt tcttaatgtt tattcgtaga tttatcgtgt tgctttttgt ttttgcttgt
    13501 attactacat tgctgtggct gatttcgtat agtcttcttt tgaagttaag tattccatta
    13561 atcttaaaag ggaaaaaagg atataagttt tcaatattta tgggtacctt caccaaatgt
    13621 atcatggtgc taatactatc ttttttactg gcacaaaatt taaatgctta tacgaagtta
    13681 aagaatatat atgactctga aaaaatctgg caaaagttag ataattacta tacattagag
    13741 atttctccta atattagaaa ttcagaagaa aaacaaattt tagaaactaa tatatacaat
    13801 ttaattaaga aaagcgaaca acttggtgga ttgcttttaa agaataataa tatagccaat
    13861 cccgataaaa acaattacat tcctgaaaat ggcaatgtct tttttatcaa taacaatttt
    13921 ctgaatttct ataaaaatat aaatcatgat tttaaaatga ttgacaatca ttcggataaa
    13981 ataaacgtgt ttattcctcc aaggttacaa tatcaaaaat ctgaaattca aaaaaatcac
    14041 caaggatgga ttgactttca aaagcaacaa aacaaaaaag tagatgtgca agtactgtca
    14101 aaaaacgtat ctgttttttc ttttgatcgc acaactaatt tgaaatttgg gtatctagat
    14161 tctccaatag taatgttgtt gacacttgac gatttaacag gagattttta tttagctgct
    14221 gtttctcaag gtggatatct atttaaagat ttagatacac ttaaacagtt gcttaaagat
    14281 aatcacctag aagaagaaat tagcggtata actaattaca aggattctgt gttaaatgaa
    14341 ttaaatgaaa cgaaaacaaa aatgataata acaatagtta ctattattat taacgttttc
    14401 attcttttga tagctactgt atttgaaaca attcaatatt ttagctggaa taaaaagcaa
    14461 ctattattaa gaaaaattca tggttattca ttattcagca gtaatgtacg ttatttaaca
    14521 atttctatct tactttctat tatgttagct tacactacgc atattttatt tggttcaaag
    14581 atattactat tcataataat gtctattgct attattcaac atttacttca aattgcttat
    14641 attaaatatc ttgaaaaata ctttaaagat ttaatgaggg agatttaaac aatgaaaaga
    14701 actttaatta ttgtaacaat tgtactatta atcatcatac ttagtctgtt attggttaga
    14761 aatgagcatt tagatcgttt taacccactg ttaaaagaaa aagtatctta tgccaaagtt
    14821 ccaaaagata cccaggacta tagaaatata actgtatact ctaaaaatgg agaaaaaaag
    14881 agttataaat tggatttttt aggttatgat cctacaaaag aatatgtgaa agtaaatcat
    14941 aaagggaaat atgtgcgttc gatagaatac attacaaaag atattcctag ctttttaaaa
    15001 tgaggtgacc gatagtgata aaacttgaaa atgtttttgt aaaaaaaggt aataaaaaca
    15061 ttttagacgg atgtaacttt aattttgaaa aaggaaaatc atatgcttta gttggagaaa
    15121 gtggcgctgg aaaatctact ttacttaaca taatcgctgg gttcgaagat gtatcacaag
    15181 gaagcatata tattgaagat aagttactaa aaaagaaagt cgatttttat agatatacgt
    15241 tagggtattt gtttcaaaat tttgctttac ttgaaaatca aacaatcagt caaaatttag
    15301 atttagccct aaagttcaaa aagaataaaa aagataatat gaacttaaag aaagaagtat
    15361 taaaaaaggt cggcctagat ttagatatca agagaattgt tagttctcta agcggtggtg
    15421 aacaacaaag agtagcattg gcaagattaa tattaaaaga tccgaaaatc attttagctg
    15481 atgaacctac tgggtcacta gatacgaaaa atggaaaaat agttattgat ttattattga
    15541 agctacttga tgaaaataaa actatgattg tcgtaacgca tgatttagag cttgcgaaac
    15601 gatttgatgt catagtcaat atttctgagc ttagaagtta agaaatattg ataggtatag
    15661 ccatacatag agaaaaaaca atatgaaata tgattagtca ttacttttga gttttttaat
    15721 ttaaggactg gattttccag tcctttttta tgatgacaaa aaacaagcct cgcagagcac
    15781 acgtttttaa tgacttattt ggtttttaat aagtctagta tgttaaaact tttctgtgtt
    15841 taggtgttat aatcatatgc agtggaagtt gatttattat gtcttattca attattcgag
    15901 ttgttaaagt gaaatcaaaa acaaatacaa gaggtattca aagacatatt caaagagaaa
    15961 ataaaaatta tgaaaatata gatattgatt taagtaagtc atatttaaac tatgatttag
    16021 tcaatgatac taagtttgat tttaataaaa aaattgatga gaaaatcgaa aaaaattata
    16081 aagggaaacg caaaattaga acagatgcca ttaaacatat tgatggatta attacatcag
    16141 ataatgtgtt ttttaatcaa ttatctgaag aagagacaaa atagtttttt aagcatgcta
    16201 aagcgttctt ggaagaagaa tacggtaaag aaaatctttt atatgcgaca gtacatatgg
    16261 atgagataac accacatatg cattatggcg tagttcctat tactaaagat ggacgtttaa
    16321 gtgcgaaaga agttgtaggt aataaaaaag cattaaccga attccaagat agattcaata
    16381 cgtatattaa taagcaaggc tatgatttaa aacgaggaat ttctagacaa cttacaaaag
    16441 aaaaacatga tcaagttagc cgttataaac aaaaaacaga atatcataaa caaatgcata
    16501 tgcgctagaa gcaaatagag gattatttaa agcaagaaaa tgataaattg atacaaaact
    16561 accaaaaatc attagatacg cttaaaaagc ttctgagtgt catgtatgaa attaaaacta
    16621 aaaatgtagg tggatggttt cataaagaga agcaagaaac aggcaatatt gtaattacta
    16681 aaactgattt tgaaaaatac actaaacaaa ttaaagcagc acagatgata ttagatgatt
    16741 atgaatgtat taaaagtggg aagtcattaa aaaaagcaga gaaacaaaat gaaagtttag
    16801 ttaatgaatt gactagtgtc catatggaaa atgaaaagct ggttgaagaa tttaatgatt
    16861 tagcacaacg ttataattat ttgttgtcag agaatgaaaa gaaagataaa gaactaaatt
    16921 atacgcttaa gttattcaat caagttttta aaatcattaa aagtatgatg aaagaagagc
    16981 gttatcatac actaataaat catattgata atcatttaga caattctaaa atacgtgaag
    17041 taatgacgat agataacaat gatgaacagt tctttaagaa gaaatatcaa gcacaagaac
    17101 gagaaatcat ttttaaagaa gatagagaag atggttatac attataaaaa tagatattta
    17161 aatgcatttg tttgaggtta attatttttt aggatgaaaa ataggtagcc cccctcgaac
    17221 tttttaatat atttttataa atctttttaa aaaccttttt aggggcttgt tcagccctag
    17281 agcaacaagg gatttcaatg cctaaaggag tgtttttgcc acataaaagg agtgtttttg
    17341 ccacataaaa ggagtgtttt tgccacataa aaggagtgtt tttgccacat aaaaggagtg
    17401 ttttaaaaag ttgtactcct tttatggttt gacattgata ataaagtagt ttattgtgaa
    17461 taaaagtttt caaatgggag tgtatattca aacgatactc ctttaggttc gtaataaagg
    17521 gggctgtatc atgacaggag agacagttgt atataaaaat gaaatgaatt tagttccatt
    17581 aaggcgattt acagctactg aaattaattt attttttgcg atgtgtaata aattaaaaga
    17641 gcaagatact aatacattac gtttgtcttt tgatgaatta aaaaaattaa gtaattatag
    17701 tccagaaaca cgtaatatta atagatttgc taatgattta gataatgtgt ataaaaagat
    17761 gttgaattta accattagat atgaagatga tgatgtaata gaaagattcg ttttattcaa
    17821 tcattataga atccataagc gagaacaata tctagaaata tcaacttctt caaatttaaa
    17881 acacatattg aattcaatta ctaataattt tacaaaattt gaattaaaag aaatgactag
    17941 gcttaaatct acatattcta aaaatatgtt tagattactt aaacaataca aacatacagg
    18001 ctatttaaaa attcacatcg atgattttaa aaatcgttta gatataccta aatcttatcg
    18061 tatgactgat attaacaaaa atgtttttaa acctattatt attgaattag gttctatatt
    18121 taataatctt actatcaata aaatcaaagc gaaaaaaggg cgtaaaattg aatggataga
    18181 gttcacattt gacgctgaga aacgcattca taacaaacga caacctcaaa tgtccaagat
    18241 agataaatca agacaatatg tgaggcgtga aaagacgcct aaatggttag aagaacggtc
    18301 atatgaaaag caacctcaaa aagattacga cccacaatta gagaaagaac gagaggattt
    18361 tttaaaacaa cttgaactga attgggaata acgcaacttt tcgttgcgag ttggttaact
    18421 aaaagtatcg tgacttttca attattttaa ttatagtttg agttaaggag tgattttatg
    18481 aaattagctg aagctataaa agaacagaga gaattaaaag gatggtctca agaggaatta
    18541 gccaatatat taaaggtttc cagacaaagt gtttctaaat gggaaagtgc caaaaactat
    18601 ccttccttag atattttgat tgctatgagc gatttattcg gaatctcttt agagcattta
    18661 attaaaggag atgcacgttt taaaaaagtt attttagagg gaaattatag agagagtaac
    18721 agggtacctt ccatagttga ttttctttat gatttttggt ggcttttctt tcctgttgcc
    18781 ggatttttag tttgggccat tcattcgttc atgggttgat tttaaaatca aaatttaatg
    18841 cacatcaggc aatatgaaaa aggaaagtat ttttacgaca ccttgaaatg tattgataag
    18901 aatagaagag tttacaaaag gcaatatgaa atatatacta aaagtaacaa gaatttcgtt
    18961 tgtaaacgca caaaaccccg aactattcgc cgtagttcgg ggttttttgg tgaattggct
    19021 ttaatcgtct atattatttt ttatcacgtt tatggagcta ataaccaaat aacgtaacga
    19081 gacaaccact gattgccgtt gtcatgatgt gaacaagaat ttcattcata aatcgcacct
    19141 cctctctacg ccgaattgac atctgagaga tagacgacct tcacattata acaaatctct
    19201 ccttccatga aataacatga atattaataa atgttgttat atattaaaga taaataaata
    19261 taagggtggg attgataatg tttggcgatc aaagacaaga agctacaaaa tatgtcatta
    19321 aagaaggtta tcaagatatt tatttcctca acaaaaacgg ggaatggtat tatttcgaag
    19381 taagaagtgt ctggcgtggc aaacatatta ttagagttaa agatggtttg ttaggttgga
    19441 gaaaagaaat agtaactgaa taaagataca aaatacttgc caatgcaaaa gaatgaaaac
    19501 gcgtcaaatc aacgatgtat aaaagatgca taaaagataa tgaaagtaaa aagccataaa
    19561 tgccgtcata taggcattta tggctttttg tttggttaac ataatcgatg attatcagaa
    19621 gttcaattca cataaaaaag cacggctaat ttatgaactt gtcgtacctt tttaattaaa
    19681 attagttacc aaatagacga ctccaaaacc cctttttagg ttccgtttct tcactctttt
    19741 cttctatatc actatcattg tcatttggat tagctgaaac gtcttctctt tgtgcttctt
    19801 caccttgtgt actttcgtcg ctttgacttc cagatatatc cttatattga atctctgttt
    19861 ctttagtttc ttcctgatct cggttaatat ttacaggact agtagtatat gttttttctt
    19921 gtgcatcaac gttttgtcta gcgttagtag atgtatcaaa gggataatta aggtgttttt
    19981 ctttttctaa ttgattttct aatttttgaa tctttttatt actctctaat gccagaactt
    20041 gctgattctc aagcaatttt gatctttctt gcaactcttt tctgtaaaat tcaattgttg
    20101 tttcatgacg actttcttgt ttttcaattt gattatttaa agtattaatc tgatttttaa
    20161 gtatttctac tatttgagca tcgcttttag attcaaaatt aatattatcc gactcttttt
    20221 tttgtttgtc ttcagtagca ctttcaaaac catatttctt tttattttca tttactcttt
    20281 gaatgatttt ttctatatcg gtatcttctt taataaacga agtgttttcc tgttttattg
    20341 tctctatatt caatctctta atattattaa aaatcgtttg cttactaacc cctaattctt
    20401 cactcaacct ttttacactt ttcatatcat caacccttta taaaatgttt ataaaccctt
    20461 agtaaaatat tataagctta taacgattaa taataaagta atcgacaaat aaaaatataa
    20521 attcgccaac ctttttatca agggtttgta aagggtttat ggtgtttatc aaaggtttat
    20581 aaacctatat tttccctttt ttaagtttat tcaaagtgct ataataacct taatataaca
    20641 aaaaccccaa ctactggtaa tagtcggggt ttggtaataa aacgtttaga aactgtttat
    20701 ttatataatt ttcgatactt agattatata
//
